MD06G1035900.v1.1

Transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0015345
Physical Location & Seq
Reverse (-)
4642129 .. 4644365
2237 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1035900.v1.1.491

Sequence Viewer

Length: 966 bp
ATGCCTTTATTAGAAGATCCTCCTTTCAACTTTATCGTCTATGATACCATCTCTGCAACCCCCTACACCAGCCAAGCTCAAGACTACTCTTCAGAAACCACCAATAATTTCATACCTCCTAAAAAATTCTACTACTGCAATAACGCTGCCGCCAAAAGTAGTAATACTAGTGTGATATTGGAAGCAGCAGGTGGCGGTGGTGGTAAGAAGACGAAGCAGATGAGCAGCAATCAGCAGCCGCAAGGGCAAGGGGAAGGGCAAGGGAGGAAGAAGAGAAGGAGAAAGGCAAGGGTTTGCAAGAACAAGGAAGAAGCTGAGACGCAGAGAATGACTCACATTGCTGTCGAAAGAAACCGCAGAAAACAAATGAACGACCATCTTGCCATTCTCCGCTCTCTCATGCCCGACTCTTACGCCCAAAGGGGTGACCAGGCCTCTATAGTAGGTGGTGCCATAGAATTCGTGAAGGAACTTGAGCATCTCCTACTGTCCCTTGAAGCTCAAAAGCTCCAACTTTTCCAACATCAACAACAGCATAATCCAGAAGATATTGTCGCCACCCCCTCCAAAATCGTGCCATCATCCCCTTTTACACAATTACTGGAATACCCTCAGAAGCACTCGTGGTCTCACAGTCATTGCCCTAACAATAAGTACACTTCAAAAACCAAGGGGGCTACCGCTGACATTGAGGTCACTTTAATTGAGACCCATGCAAACCTTAAAATTCTCTCACGCAAAAATATTGCAGCAAGATCACAGCTTTCCAAGTTGATCGCTGGAATTCATTCACTTCACCTCACAATCCTTCATCTCACTGTTTCAACCGTGGATCCATTTGTTCTCTACTCTATTAGTCTCAAGATTGAAGAAGGGTGCTTACTAAGTTCGGCAGATGACATAGCAGCATCACTTCACCACATGCTTAGAGTGATAGAGGAAGCAGCTGCCAGATTATTGTGTTAA

Protein Analysis

322

Amino Acids

35.74

Weight (kDa)

9.06

Isoelectric Point (pI)

58.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 108 - 159 4.5e-13 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015345)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G46690 AT5G46690
fragaria_vesca FvH4_5g22360
malus_domestica MD06G1035900.v1.1
prunus_persica Prupe.5G033300_v2.0.a1
pyrus_communis pycom06g02970
rosa_chinensis RchiOBHm_Chr7g0210101
rosa_laevigata RLG00000003065
rosa_multiflora Rmu_sc0001656.1_g000007
rosa_roxburghii Rroxscaffold_3G00248620
rosa_rugosa Rorug07G0118500
rosa_samantha Rh7AG252000 Rh7BG246800 Rh7CG269000 Rh7DG259300
rosa_wichuraiana Rw7G021410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 179
AasI GACNNNNNNGTC 1 cut(s) 692
Acc36I ACCTGC 1 cut(s) 179
AccB1I GGYRCC 1 cut(s) 449
AccBSI CCGCTC 1 cut(s) 393
AciI CCGC 6 cut(s) 150, 195, 239, 355, 391, 681
AclWI GGATC 3 cut(s) 11, 827, 840
AcsI RAATTY 4 cut(s) 125, 458, 726, 783
AcuI CTGAAG 1 cut(s) 75
AfaI GTAC 1 cut(s) 656
AgsI TTSAA 5 cut(s) 28, 497, 663, 825, 869
AhlI ACTAGT 1 cut(s) 167
AjnI CCWGG 1 cut(s) 429
AluBI AGCT 6 cut(s) 77, 314, 500, 508, 763, 947
AluI AGCT 6 cut(s) 77, 314, 500, 508, 763, 947
Alw26I GTCTC 4 cut(s) 311, 633, 701, 863
AlwI GGATC 3 cut(s) 11, 827, 840
AoxI GGCC 1 cut(s) 432
ApeKI GCWGC 8 cut(s) 146, 185, 225, 235, 749, 905, 944, 947
ApoI RAATTY 4 cut(s) 125, 458, 726, 783
Asp700I GAANNNNTTC 1 cut(s) 787
AsuHPI GGTGA 3 cut(s) 437, 788, 908
BamHI GGATCC 1 cut(s) 832
BanI GGYRCC 1 cut(s) 449
BarI GAAGNNNNNNTAC 2 cut(s) 864, 896
BauI CACGAG 1 cut(s) 622
BbsI GAAGAC 1 cut(s) 215
BbvI GCAGC 8 cut(s) 133, 197, 237, 247, 761, 917, 934, 956
BccI CCATC 3 cut(s) 56, 384, 586
BcgI CGANNNNNNTGC 2 cut(s) 362, 396
BciT130I CCWGG 1 cut(s) 431
BcoDI GTCTC 4 cut(s) 311, 633, 701, 863
BcuI ACTAGT 1 cut(s) 167
BfaI CTAG 1 cut(s) 168
BfmI CTRYAG 1 cut(s) 438
BfuAI ACCTGC 1 cut(s) 179
BglI GCCNNNNNGGC 1 cut(s) 244
Bme1390I CCNGG 1 cut(s) 431
BmiI GGNNCC 2 cut(s) 451, 834
BmrFI CCNGG 1 cut(s) 431
BmsI GCATC 2 cut(s) 487, 917
BpiI GAAGAC 1 cut(s) 215
BplI GAGNNNNNCTC 2 cut(s) 316, 348
BpuEI CTTGAG 3 cut(s) 63, 494, 845
BsaI GGTCTC 2 cut(s) 633, 701
BsaJI CCNNGG 2 cut(s) 669, 828
Bse1I ACTGG 1 cut(s) 606
Bse3DI GCAATG 2 cut(s) 336, 637
BseBI CCWGG 1 cut(s) 431
BseDI CCNNGG 2 cut(s) 669, 828
BseGI GGATG 1 cut(s) 581
BseMI GCAATG 2 cut(s) 336, 637
BseMII CTCAG 2 cut(s) 306, 626
BseNI ACTGG 1 cut(s) 606
BseXI GCAGC 8 cut(s) 133, 197, 237, 247, 761, 917, 934, 956
BshFI GGCC 1 cut(s) 434
BshNI GGYRCC 1 cut(s) 449
BslFI GGGAC 1 cut(s) 475
BsmAI GTCTC 4 cut(s) 311, 633, 701, 863
BsmBI CGTCTC 1 cut(s) 311
BsmFI GGGAC 1 cut(s) 475
BsnI GGCC 1 cut(s) 434
Bso31I GGTCTC 2 cut(s) 633, 701
Bsp143I GATC 4 cut(s) 16, 755, 774, 832
BspACI CCGC 6 cut(s) 150, 195, 239, 355, 391, 681
BspANI GGCC 1 cut(s) 434
BspCNI CTCAG 2 cut(s) 307, 625
BspLI GGNNCC 2 cut(s) 451, 834
BspMI ACCTGC 1 cut(s) 179
BspPI GGATC 3 cut(s) 11, 827, 840
BspT107I GGYRCC 1 cut(s) 449
BspTNI GGTCTC 2 cut(s) 633, 701
BsrBI CCGCTC 1 cut(s) 393
BsrDI GCAATG 2 cut(s) 336, 637
BsrI ACTGG 1 cut(s) 606
BssECI CCNNGG 2 cut(s) 669, 828
BssMI GATC 4 cut(s) 16, 755, 774, 832
BssSI CACGAG 1 cut(s) 622
BssT1I CCWWGG 1 cut(s) 669
Bst2BI CACGAG 1 cut(s) 622
Bst2UI CCWGG 1 cut(s) 431
Bst4CI ACNGT 4 cut(s) 489, 635, 820, 829
Bst6I CTCTTC 2 cut(s) 94, 266
BstDEI CTNAG 4 cut(s) 315, 612, 884, 926
BstDSI CCRYGG 1 cut(s) 828
BstEII GGTNACC 1 cut(s) 425
BstF5I GGATG 1 cut(s) 581
BstKTI GATC 4 cut(s) 19, 758, 777, 835
BstMAI GTCTC 4 cut(s) 311, 633, 701, 863
BstMBI GATC 4 cut(s) 16, 755, 774, 832
BstMWI GCNNNNNNNGC 1 cut(s) 244
BstNI CCWGG 1 cut(s) 431
BstNSI RCATGY 1 cut(s) 925
BstPI GGTNACC 1 cut(s) 425
BstSCI CCNGG 1 cut(s) 429
BstSFI CTRYAG 1 cut(s) 438
BstV1I GCAGC 8 cut(s) 133, 197, 237, 247, 761, 917, 934, 956
BstV2I GAAGAC 1 cut(s) 215
BstX2I RGATCY 2 cut(s) 16, 832
BstYI RGATCY 2 cut(s) 16, 832
BsuRI GGCC 1 cut(s) 434
BtgI CCRYGG 1 cut(s) 828
BtsCI GGATG 1 cut(s) 581
BtsIMutI CAGTG 1 cut(s) 816
BveI ACCTGC 1 cut(s) 179
CseI GACGC 1 cut(s) 328
Csp6I GTAC 1 cut(s) 655
CviAII CATG 3 cut(s) 400, 713, 922
CviQI GTAC 1 cut(s) 655
DdeI CTNAG 4 cut(s) 315, 612, 884, 926
DpnI GATC 4 cut(s) 18, 757, 776, 834
DpnII GATC 4 cut(s) 16, 755, 774, 832
DrdI GACNNNNNNGTC 1 cut(s) 692
DseDI GACNNNNNNGTC 1 cut(s) 692
Eam1104I CTCTTC 2 cut(s) 94, 266
EarI CTCTTC 2 cut(s) 94, 266
Eco130I CCWWGG 1 cut(s) 669
Eco147I AGGCCT 1 cut(s) 434
Eco31I GGTCTC 2 cut(s) 633, 701
Eco57I CTGAAG 1 cut(s) 75
Eco91I GGTNACC 1 cut(s) 425
EcoO65I GGTNACC 1 cut(s) 425
EcoRI GAATTC 2 cut(s) 458, 783
EcoRII CCWGG 1 cut(s) 429
EcoT14I CCWWGG 1 cut(s) 669
ErhI CCWWGG 1 cut(s) 669
Esp3I CGTCTC 1 cut(s) 311
FaeI CATG 3 cut(s) 403, 716, 925
FaiI YATR 9 cut(s) 42, 113, 401, 440, 455, 537, 714, 902, 923
FaqI GGGAC 1 cut(s) 475
FatI CATG 3 cut(s) 399, 712, 921
FokI GGATG 1 cut(s) 568
FspBI CTAG 1 cut(s) 168
HaeIII GGCC 1 cut(s) 434
HgaI GACGC 1 cut(s) 328
Hin1II CATG 3 cut(s) 403, 716, 925
HinfI GANTC 2 cut(s) 331, 407
HphI GGTGA 3 cut(s) 437, 788, 908
Hpy166II GTNNAC 1 cut(s) 657
Hpy188I TCNGA 2 cut(s) 94, 615
Hpy188III TCNNGA 4 cut(s) 80, 463, 542, 862
Hpy8I GTNNAC 1 cut(s) 657
HpyAV CCTTC 5 cut(s) 248, 270, 460, 818, 866
HpyCH4III ACNGT 4 cut(s) 489, 635, 820, 829
HpyCH4V TGCA 5 cut(s) 56, 138, 297, 716, 749
HpyF10VI GCNNNNNNNGC 1 cut(s) 244
HpyF3I CTNAG 4 cut(s) 315, 612, 884, 926
Hsp92II CATG 3 cut(s) 403, 716, 925
Kzo9I GATC 4 cut(s) 16, 755, 774, 832
LmnI GCTCC 1 cut(s) 513
LpnPI CCDG 7 cut(s) 82, 174, 416, 443, 555, 587, 765
Lsp1109I GCAGC 8 cut(s) 133, 197, 237, 247, 761, 917, 934, 956
LweI GCATC 2 cut(s) 487, 917
MaeI CTAG 1 cut(s) 168
MaeIII GTNAC 2 cut(s) 425, 694
MalI GATC 4 cut(s) 18, 757, 776, 834
MbiI CCGCTC 1 cut(s) 393
MboI GATC 4 cut(s) 16, 755, 774, 832
MboII GAAGA 8 cut(s) 26, 81, 220, 280, 283, 320, 557, 881
MflI RGATCY 2 cut(s) 16, 832
MluCI AATT 7 cut(s) 106, 125, 458, 596, 702, 726, 783
MlyI GAGTC 2 cut(s) 325, 401
MmeI TCCRAC 2 cut(s) 535, 544
MnlI CCTC 9 cut(s) 30, 126, 258, 445, 574, 621, 685, 809, 931
MroXI GAANNNNTTC 1 cut(s) 787
MseI TTAA 3 cut(s) 701, 723, 964
MspA1I CMGCKG 2 cut(s) 683, 947
MspR9I CCNGG 1 cut(s) 431
MvaI CCWGG 1 cut(s) 431
MwoI GCNNNNNNNGC 1 cut(s) 244
NdeII GATC 4 cut(s) 16, 755, 774, 832
NlaIII CATG 3 cut(s) 403, 716, 925
NlaIV GGNNCC 2 cut(s) 451, 834
NmuCI GTSAC 2 cut(s) 425, 694
NspI RCATGY 1 cut(s) 925
PaqCI CACCTGC 1 cut(s) 179
PceI AGGCCT 1 cut(s) 434
PdmI GAANNNNTTC 1 cut(s) 787
PleI GAGTC 2 cut(s) 325, 401
PpsI GAGTC 2 cut(s) 325, 401
Psp6I CCWGG 1 cut(s) 429
PspEI GGTNACC 1 cut(s) 425
PspGI CCWGG 1 cut(s) 429
PspN4I GGNNCC 2 cut(s) 451, 834
PsuI RGATCY 2 cut(s) 16, 832
PvuII CAGCTG 1 cut(s) 947
RsaI GTAC 1 cut(s) 656
RsaNI GTAC 1 cut(s) 655
SaqAI TTAA 3 cut(s) 701, 723, 964
Sau3AI GATC 4 cut(s) 16, 755, 774, 832
SchI GAGTC 2 cut(s) 325, 401
ScrFI CCNGG 1 cut(s) 431
SfaNI GCATC 2 cut(s) 487, 917
SfcI CTRYAG 1 cut(s) 438
SmlI CTYRAG 3 cut(s) 78, 473, 860
SmoI CTYRAG 3 cut(s) 78, 473, 860
SpeI ACTAGT 1 cut(s) 167
Sse9I AATT 7 cut(s) 106, 125, 458, 596, 702, 726, 783
SseBI AGGCCT 1 cut(s) 434
SsiI CCGC 6 cut(s) 150, 195, 239, 355, 391, 681
SspI AATATT 1 cut(s) 745
SspMI CTAG 1 cut(s) 168
StuI AGGCCT 1 cut(s) 434
StyD4I CCNGG 1 cut(s) 429
StyI CCWWGG 1 cut(s) 669
TaaI ACNGT 4 cut(s) 489, 635, 820, 829
TaqI TCGA 1 cut(s) 345
TasI AATT 7 cut(s) 106, 125, 458, 596, 702, 726, 783
TatI WGTACW 1 cut(s) 654
TauI GCSGC 2 cut(s) 152, 241
Tru1I TTAA 3 cut(s) 701, 723, 964
Tru9I TTAA 3 cut(s) 701, 723, 964
TscAI CASTG 1 cut(s) 823
TseFI GTSAC 2 cut(s) 425, 694
TseI GCWGC 8 cut(s) 146, 185, 225, 235, 749, 905, 944, 947
Tsp45I GTSAC 2 cut(s) 425, 694
TspDTI ATGAA 4 cut(s) 100, 383, 776, 800
TspRI CASTG 1 cut(s) 823
XapI RAATTY 4 cut(s) 125, 458, 726, 783
XceI RCATGY 1 cut(s) 925
XmnI GAANNNNTTC 1 cut(s) 787
XspI CTAG 1 cut(s) 168
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.