Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0014005

Physical Location & Seq

Genomic Coordinates Forward (+)

6321710 .. 6327268

Gene Structure 5559 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1046600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3174 bp

ATGGTCCAATTCCACGAGCACATCATAACCGAACTCCTCGACGATCCTCAAAACGGCAGCGGCCTCGTCGTTCTCTCCTCCGGCCTCTCCCTCCCCAGACTCATCGCCGCCCTCCTCCTCCTCCACACTCCTTCTCAGGGCACCCTCCTCATCCTTTCCCCCCACCAACCTTTCTTCAAATCCCAACTCCTCCACCACTTCCACCCCAACCCCATCCCCGAAATCTCCGCCGACCTCCCCTCCCACCACCGCCACTCCCTATACACCTCCTCCAATGTCTTCTTCATCACTCCCCGCATCCTCATCGTCGACCTCCTCACCAGCAAATTACCCACCTCCCAAATCGCTGGAATCATCATCCCCACCGTTCATTCCCTCACCGAAACCTCCACGGAAGCATTTATCGTCCGGATTTTCCGCTCCCTCAACAAAACGGCGTTTGTTCGCGCGTTTTCTGACAAACCCCATTCCATGGTTTCTGGGTTTGCCAAGGCGGAGAGGATTATGAAGTGCTTGTACCTCCGGAAGATGCACCTCTGGCCGAGGTTCCATGTGGATGTGTCGCAGGAGCTGGAAAGGCAGCCGCCGGAGGTGGTGGATATACGGGTGCCAATGACCAAACACATGGTGGGAATTCAGAAGGCGATAATTGAGGTGATGGATGCTTGCTTGAAGGAGATGAGGAAGACCAATAAGGTTGATGTGGAGGATTTGACGGTGGAGAACGGGTTGTTTAAGTCGTTTGATGAGATTGTGAGGAGGCAATTGGACCCCATTTGGCATACCTTGGGGAAGAAGACTAAGCAGCTCGTCTCCGATTTAAAGACTCTTAGAAAGCTCTTGGATTATCTTGTCAGATATGATGCGGTAACTTATTTGAAATACTTGGATACACTACGCGTGTCAGAGAATTTTCGATCAGTCTGGATATTTGCAGAGTCGAGCTATAAGATATTTGAGTATTCAAAGAAAAGGGTGTATCGGTTTGTGACATCGGATGGTGTGAAATTAAGTGATAAGAGTAAGAGTTCGAAAGGCAAGAAAAGAAAACTGAAAGGGGATGATGATAATGAGCAAGTTGGAGGCACGTCGTCGACCAGTACAAGTAGTAGGATAGTTTTAGATGAAGTTTTAGAGGAGGCACCAAAGTGGAAGGTTTTACGTGAGGTTCTTGAAGAGATAGAAGAGGAAAGGCGACAGGCTTCGTCAAGAGAGGAACTTTTGGTTGAAGGTGATGACGATTATAGTGGTATTGTTCTAGTGGCATGCAAAGATGAACGTTCATGCATGCAGCTTGAAGATTTCATCACCAATGGACCACAGAAGGTCATGCGGGAGGAATGGGAAAACTACTTGCTAGGAAAGGGAGAGCTCCGTGACTTGCATACACGGTATAAAAAGAAATCAAAGAATCCCAAAGGTTTTGGGGTTCTAGATGGAGTTGTTCACGTAGCACCCCCACAGAATGCAGAAGCTAGCAGTGTAAACAAGCAGGAACACGATGCCCTTTTGGCTGCAGCTGCTGAAATTAGAAAGCGAGCTAAAAAAGATGATGCTGTTGGAGATGATCCTCAGCTTCTTGTTAGTGGTAGAGGACATGGAAAGGGAAGGGGAAAGGGAAAAGTAGTTCCTTCAAAATCCCCACAGAACGCAGAAGCTGGCCGCATAAACAACCAGGAACATGATGTGCTTTTGGCTTCATCGTCAGAAATTCAAAGGAAAGCCGAAAAAGATGATGATGTTGGAGCTGATCCTCCTCTTCTTGTTAGTGGCAAAGGACGTGGAAAGGGTAGGGGAAAAGTGAGGACCAAAAACAGCCTTGCCAAGGTTAAATGTTTGGGGACTAAAGCCAGTACTAATGATAAAAAAGAAGTCATACCTGATGATAAAGCCAGTACTAATGATAAAAAAGAAGTCATACCTGATGATCTTGTTATACCGGATTCGGAGGATGAAGGACAAGAATATGAACTAAATCAGGTGGTGACTGCTGATTCTACTGAACCTACATGCCAGATTGACCCTGTAGATGAAGGGGTGTTGAGGAAGCATACTGGAGTAGCGGATTCAACTGGAACAATAAATGCTAAGCCACTACCTCCAGTTCACTTTTATGCTCTAGAAAGTGGTCAGCCTATACTGGACATTTTGAGACCCTCTATTATCATTGTTTACCACCCAGATATGACTTTTGTCAGAGAAATTGAAGTTCATAAAGCTGAGAACCCCTCGAAGTTGAAAGTTTATTTTATTTTCTATGAAGATTCTTCTGAGGTGCAAAAGTTTGAGGCAAGTATAAGAAGGGAAAATGGAGCGTTTGAATCTTTGATTAGGCAGAAGTCACTAATGATGATACCAGTTGATCAGGATGGGAACTGCTTGGGATTGAATTCTTCCCAAGAACCACAAGCTTTGTCATCCCAAAATTCTTTAACCAGAAAAGCGGGGGGAAGAAAGGTTGCTGAGAAGGAAATGCAGGTCATAGTGGATATGAGGGAGTTCATGAGCAGCCTTCCAAATGTTCTTCACCAGAAAGGCATGCGCATAATACCCGTAACTTTGGAAGTTGGGGATTATATCCTTTCTCCACTGATATGTGTGGAGAGAAAGAGTATCCAAGATCTCTTTATGAGTTTCACATCAGGTCGTCTTTATCACCAAGTGGAAACAATGGTTCGATACTATAGAATACCTGTTCTCCTGATTGAATTTTCACAGGATAAAAGTTTCTCCTTTCAGTCCGCAAGTGATATTGGTGATGATGTCACACCAAATAGTATTATATCTAAGCTGTCGTTGCTTGTTCTACACTTCCCCCGGTTGCGAATCATTTGGTCCCGCAGTCTGCATGCTACTGCTGACATATTTGCTACTCTTAAGGCAAATCAGGATGAACCTGACGAGAGCAAAGCAACTAGAGTTGGTGTACCCTCTGAAGATGGAATTGTGGAGGATGATGTGAGGGCTGAAAACTACAATACATCTGCGGTGGAGTTTTTGAGACGACTCCCAGGTGTTACAGATTCAAATTACAGGGCCATAATGGATGGGTGTAAGAGCTTGGCAGAACTCGCTCTTATGCCGGTGGAGAAGCTAGCGGAACTAATGGGCGGTCAGAAAGCTGCTCGCATTCTCAGAGACTTTCTTGATGCCAAGTATCCAACTCTGTTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000014 GO:0000109 GO:0000110 GO:0000280 GO:0000712 GO:0000724 GO:0000725 GO:0003674 GO:0003676 GO:0003677 GO:0003684 GO:0003697 GO:0003824 GO:0004518 GO:0004519 GO:0004520 GO:0004536 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006281 GO:0006289 GO:0006296 GO:0006302 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006979 GO:0006996 GO:0007049 GO:0007059 GO:0007127 GO:0007131 GO:0008150 GO:0008152 GO:0009314 GO:0009628 GO:0009987 GO:0016043 GO:0016787 GO:0016788 GO:0022402 GO:0022414 GO:0032991 GO:0033554 GO:0033683 GO:0034641 GO:0035825 GO:0036297 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045132 GO:0046483 GO:0048285 GO:0050896 GO:0051304 GO:0051307 GO:0051321 GO:0051716 GO:0061982 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:0097159 GO:0098813 GO:0140013 GO:0140097 GO:1901255 GO:1901360 GO:1901363 GO:1903046 GO:1990391

KEGG Pathways

Metabolic & Signaling

ko03420 ko03460 map03420 map03460

Pfam Domains

Protein Families

ERCC4 Pro_isomerase

Protein Analysis

1058

Amino Acids

118.72

Weight (kDa)

6.68

Isoelectric Point (pI)

37.25

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ERCC4	PF02732	829 - 958	1.3e-28	ERCC4 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0014005)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G41150 AT5G41150
fragaria_vesca	FvH4_5g20470
malus_domestica	MD06G1046600.v1.1
prunus_persica	Prupe.5G054900_v2.0.a1 Prupe.5G054900_v2.0.a1
pyrus_communis	pycom06g03930
rosa_chinensis	RchiOBHm_Chr7g0205791
rosa_laevigata	RLG00000003354
rosa_multiflora	Rmu_sc0001336.1_g000030
rosa_roxburghii	Rroxscaffold_3G00251980
rosa_rugosa	Rorug07G0093100
rosa_samantha	Rh7AG224600 Rh7BG219800 Rh7CG238300 Rh7DG231300
rosa_wichuraiana	Rw7G019370

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	2543
Acc36I	ACCTGC	1 cut(s)	2467
AccB1I	GGYRCC	3 cut(s)	140, 607, 1141
AccB7I	CCANNNNNTGG	1 cut(s)	472
AccBSI	CCGCTC	1 cut(s)	420
AccI	GTMKAC	2 cut(s)	309, 1094
AccII	CGCG	3 cut(s)	447, 449, 900
AccIII	TCCGGA	2 cut(s)	408, 522
AclI	AACGTT	1 cut(s)	1279
AclWI	GGATC	3 cut(s)	38, 1562, 1745
AcoI	YGGCCR	2 cut(s)	539, 1660
AcsI	RAATTY	6 cut(s)	633, 910, 1710, 2389, 2425, 2708
AcuI	CTGAAG	1 cut(s)	2955
AdeI	CACNNNGTG	2 cut(s)	628, 2662
AfaI	GTAC	5 cut(s)	518, 1102, 1855, 1897, 2928
AfiI	CCNNNNNNNGG	4 cut(s)	53, 137, 472, 1825
AflII	CTTAAG	1 cut(s)	2876
AflIII	ACRYGT	1 cut(s)	898
AjiI	CACGTC	2 cut(s)	1089, 1781
AjnI	CCWGG	2 cut(s)	1674, 3010
AjuI	GAANNNNNNNTTGG	2 cut(s)	266, 298
AleI	CACNNNNGTG	1 cut(s)	1147
AloI	GAACNNNNNNTCC	2 cut(s)	1611, 1643
Alw21I	GWGCWC	2 cut(s)	21, 1374
Alw26I	GTCTC	4 cut(s)	817, 2146, 2995, 3132
AlwI	GGATC	3 cut(s)	38, 1562, 1745
AlwNI	CAGNNNCTG	3 cut(s)	571, 1523, 1658
Aor13HI	TCCGGA	2 cut(s)	408, 522
AoxI	GGCC	5 cut(s)	61, 82, 539, 1660, 3036
ApeKI	GCWGC	9 cut(s)	57, 580, 805, 1291, 1514, 1517, 1520, 2508, 3122
ApoI	RAATTY	6 cut(s)	633, 910, 1710, 2389, 2425, 2708
AspLEI	GCGC	2 cut(s)	449, 2544
AspS9I	GGNCC	6 cut(s)	4, 769, 1316, 1806, 2835, 3036
AsuC2I	CCSGG	1 cut(s)	2818
AsuHPI	GGTGA	9 cut(s)	310, 370, 667, 1244, 1300, 1996, 2519, 2648, 2768
AsuII	TTCGAA	1 cut(s)	1031
AsuNHI	GCTAGC	2 cut(s)	1475, 3094
AvaII	GGWCC	5 cut(s)	4, 769, 1316, 1806, 2835
BaeGI	GKGCMC	1 cut(s)	143
BanI	GGYRCC	3 cut(s)	140, 607, 1141
BanII	GRGCYC	1 cut(s)	1374
BarI	GAAGNNNNNNTAC	2 cut(s)	500, 532
BauI	CACGAG	1 cut(s)	14
BbsI	GAAGAC	3 cut(s)	271, 692, 803
Bbv12I	GWGCWC	2 cut(s)	21, 1374
BbvCI	CCTCAGC	1 cut(s)	1572
BbvI	GCAGC	9 cut(s)	69, 592, 817, 1303, 1501, 1507, 1529, 2520, 3109
BccI	CCATC	7 cut(s)	221, 652, 992, 1430, 2363, 2933, 3041
BceAI	ACGGC	2 cut(s)	70, 450
BcgI	CGANNNNNNTGC	4 cut(s)	46, 80, 286, 320
BciT130I	CCWGG	2 cut(s)	1676, 3012
BciVI	GTATCC	3 cut(s)	883, 2624, 3168
BclI	TGATCA	1 cut(s)	2362
BcnI	CCSGG	1 cut(s)	2818
BcoDI	GTCTC	4 cut(s)	817, 2146, 2995, 3132
BfaI	CTAG	7 cut(s)	1259, 1358, 1433, 1476, 2120, 2916, 3095
BfmI	CTRYAG	3 cut(s)	1515, 2025, 2683
BfrI	CTTAAG	1 cut(s)	2876
BfuAI	ACCTGC	1 cut(s)	2467
BfuI	GTATCC	3 cut(s)	883, 2624, 3168
BglI	GCCNNNNNGGC	1 cut(s)	1511
BglII	AGATCT	1 cut(s)	2620
BlpI	GCTNAGC	1 cut(s)	2088
BmcAI	AGTACT	2 cut(s)	1855, 1897
Bme1390I	CCNGG	3 cut(s)	1676, 2818, 3012
Bme18I	GGWCC	5 cut(s)	4, 769, 1316, 1806, 2835
BmgBI	CACGTC	2 cut(s)	1089, 1781
BmgT120I	GGNCC	6 cut(s)	4, 769, 1316, 1806, 2835, 3036
BmiI	GGNNCC	6 cut(s)	142, 548, 609, 771, 1143, 2837
BmrFI	CCNGG	3 cut(s)	1676, 2818, 3012
BmsI	GCATC	7 cut(s)	306, 519, 652, 853, 1492, 1543, 3139
BmtI	GCTAGC	2 cut(s)	1479, 3098
BoxI	GACNNNNGTC	1 cut(s)	2192
BpiI	GAAGAC	3 cut(s)	271, 692, 803
BplI	GAGNNNNNCTC	2 cut(s)	2213, 2245
BpmI	CTGGAG	2 cut(s)	2076, 2085
Bpu10I	CCTNAGC	1 cut(s)	1572
Bpu1102I	GCTNAGC	1 cut(s)	2088
Bpu14I	TTCGAA	1 cut(s)	1031
BpuMI	CCSGG	1 cut(s)	2818
BsaAI	YACGTR	2 cut(s)	1163, 1450
BsaI	GGTCTC	1 cut(s)	2146
BsaJI	CCNNGG	8 cut(s)	390, 471, 489, 542, 786, 1824, 2816, 3010
BsaWI	WCCGGW	3 cut(s)	408, 522, 1939
Bsc4I	CCNNNNNNNGG	4 cut(s)	53, 137, 472, 1825
Bse118I	RCCGGY	1 cut(s)	3082
Bse1I	ACTGG	8 cut(s)	1098, 1851, 1893, 2059, 2077, 2102, 2145, 2357
BseAI	TCCGGA	2 cut(s)	408, 522
BseBI	CCWGG	2 cut(s)	1676, 3012
BseDI	CCNNGG	8 cut(s)	390, 471, 489, 542, 786, 1824, 2816, 3010
BseLI	CCNNNNNNNGG	4 cut(s)	53, 137, 472, 1825
BseMII	CTCAG	6 cut(s)	149, 1586, 2211, 2262, 2454, 3148
BseNI	ACTGG	8 cut(s)	1098, 1851, 1893, 2059, 2077, 2102, 2145, 2357
BseSI	GKGCMC	1 cut(s)	143
BseXI	GCAGC	9 cut(s)	69, 592, 817, 1303, 1501, 1507, 1529, 2520, 3109
Bsh1236I	CGCG	3 cut(s)	447, 449, 900
BshFI	GGCC	5 cut(s)	63, 84, 541, 1662, 3038
BshNI	GGYRCC	3 cut(s)	140, 607, 1141
BsiHKAI	GWGCWC	2 cut(s)	21, 1374
BsiSI	CCGG	7 cut(s)	81, 409, 523, 587, 1940, 2818, 3083
BslFI	GGGAC	2 cut(s)	1855, 2821
BslI	CCNNNNNNNGG	4 cut(s)	53, 137, 472, 1825
BsmAI	GTCTC	4 cut(s)	817, 2146, 2995, 3132
BsmBI	CGTCTC	2 cut(s)	817, 2995
BsmFI	GGGAC	2 cut(s)	1855, 2821
BsmI	GAATGC	2 cut(s)	1471, 3129
BsnI	GGCC	5 cut(s)	63, 84, 541, 1662, 3038
Bso31I	GGTCTC	1 cut(s)	2146
Bsp119I	TTCGAA	1 cut(s)	1031
Bsp1286I	GDGCHC	3 cut(s)	21, 143, 1374
Bsp13I	TCCGGA	2 cut(s)	408, 522
Bsp143I	GATC	7 cut(s)	43, 917, 1567, 1750, 1927, 2362, 2620
Bsp1720I	GCTNAGC	1 cut(s)	2088
Bsp19I	CCATGG	1 cut(s)	471
BspANI	GGCC	5 cut(s)	63, 84, 541, 1662, 3038
BspCNI	CTCAG	6 cut(s)	148, 1585, 2212, 2263, 2455, 3147
BspEI	TCCGGA	2 cut(s)	408, 522
BspFNI	CGCG	3 cut(s)	447, 449, 900
BspHI	TCATGA	1 cut(s)	2502
BspLI	GGNNCC	6 cut(s)	142, 548, 609, 771, 1143, 2837
BspMAI	CTGCAG	1 cut(s)	1519
BspMI	ACCTGC	1 cut(s)	2467
BspOI	GCTAGC	2 cut(s)	1479, 3098
BspPI	GGATC	3 cut(s)	38, 1562, 1745
BspT104I	TTCGAA	1 cut(s)	1031
BspT107I	GGYRCC	3 cut(s)	140, 607, 1141
BspTI	CTTAAG	1 cut(s)	2876
BspTNI	GGTCTC	1 cut(s)	2146
BsrBI	CCGCTC	1 cut(s)	420
BsrFI	RCCGGY	1 cut(s)	3082
BsrI	ACTGG	8 cut(s)	1098, 1851, 1893, 2059, 2077, 2102, 2145, 2357
BssAI	RCCGGY	1 cut(s)	3082
BssECI	CCNNGG	8 cut(s)	390, 471, 489, 542, 786, 1824, 2816, 3010
BssMI	GATC	7 cut(s)	43, 917, 1567, 1750, 1927, 2362, 2620
BssSI	CACGAG	1 cut(s)	14
BssT1I	CCWWGG	4 cut(s)	471, 489, 786, 1824
Bst2BI	CACGAG	1 cut(s)	14
Bst2UI	CCWGG	2 cut(s)	1676, 3012
Bst4CI	ACNGT	3 cut(s)	367, 718, 1392
Bst6I	CTCTTC	3 cut(s)	1170, 1179, 1764
BstAFI	CTTAAG	1 cut(s)	2876
BstBAI	YACGTR	2 cut(s)	1163, 1450
BstBI	TTCGAA	1 cut(s)	1031
BstDSI	CCRYGG	2 cut(s)	390, 471
BstENI	CCTNNNNNAGG	1 cut(s)	1823
BstFNI	CGCG	3 cut(s)	447, 449, 900
BstHHI	GCGC	2 cut(s)	449, 2544
BstKTI	GATC	7 cut(s)	46, 920, 1570, 1753, 1930, 2365, 2623
BstMAI	GTCTC	4 cut(s)	817, 2146, 2995, 3132
BstMBI	GATC	7 cut(s)	43, 917, 1567, 1750, 1927, 2362, 2620
BstMWI	GCNNNNNNNGC	6 cut(s)	538, 577, 1511, 1520, 2797, 3071
BstNI	CCWGG	2 cut(s)	1676, 3012
BstNSI	RCATGY	5 cut(s)	1269, 1291, 2013, 2542, 2852
BstPAI	GACNNNNGTC	1 cut(s)	2192
BstSCI	CCNGG	3 cut(s)	1674, 2816, 3010
BstSFI	CTRYAG	3 cut(s)	1515, 2025, 2683
BstSLI	GKGCMC	1 cut(s)	143
BstUI	CGCG	3 cut(s)	447, 449, 900
BstV1I	GCAGC	9 cut(s)	69, 592, 817, 1303, 1501, 1507, 1529, 2520, 3109
BstV2I	GAAGAC	3 cut(s)	271, 692, 803
BstX2I	RGATCY	1 cut(s)	2620
BstXI	CCANNNNNNTGG	2 cut(s)	347, 625
BstYI	RGATCY	1 cut(s)	2620
BsuI	GTATCC	3 cut(s)	883, 2624, 3168
BsuRI	GGCC	5 cut(s)	63, 84, 541, 1662, 3038
BtgI	CCRYGG	2 cut(s)	390, 471
BtgZI	GCGATG	1 cut(s)	88
BtrI	CACGTC	2 cut(s)	1089, 1781
BtsI	GCAGTG	1 cut(s)	1486
BtsIMutI	CAGTG	2 cut(s)	1486, 2588
BveI	ACCTGC	1 cut(s)	2467
CaiI	CAGNNNCTG	3 cut(s)	571, 1523, 1658
CciI	TCATGA	1 cut(s)	2502
CfoI	GCGC	2 cut(s)	449, 2544
Cfr10I	RCCGGY	1 cut(s)	3082
Cfr13I	GGNCC	6 cut(s)	4, 769, 1316, 1806, 2835, 3036
Csp6I	GTAC	5 cut(s)	517, 1101, 1854, 1896, 2927
CspCI	CAANNNNNGTGG	4 cut(s)	1762, 1797, 2394, 2429
CviQI	GTAC	5 cut(s)	517, 1101, 1854, 1896, 2927
DpnI	GATC	7 cut(s)	45, 919, 1569, 1752, 1929, 2364, 2622
DpnII	GATC	7 cut(s)	43, 917, 1567, 1750, 1927, 2362, 2620
DraI	TTTAAA	1 cut(s)	822
DraIII	CACNNNGTG	2 cut(s)	628, 2662
EaeI	YGGCCR	2 cut(s)	539, 1660
Eam1104I	CTCTTC	3 cut(s)	1170, 1179, 1764
EarI	CTCTTC	3 cut(s)	1170, 1179, 1764
EciI	GGCGGA	2 cut(s)	217, 509
Ecl136II	GAGCTC	1 cut(s)	1372
Eco130I	CCWWGG	4 cut(s)	471, 489, 786, 1824
Eco24I	GRGCYC	1 cut(s)	1374
Eco31I	GGTCTC	1 cut(s)	2146
Eco47I	GGWCC	5 cut(s)	4, 769, 1316, 1806, 2835
Eco53kI	GAGCTC	1 cut(s)	1372
Eco57I	CTGAAG	1 cut(s)	2955
EcoICRI	GAGCTC	1 cut(s)	1372
EcoNI	CCTNNNNNAGG	1 cut(s)	1823
EcoRI	GAATTC	2 cut(s)	633, 2389
EcoRII	CCWGG	2 cut(s)	1674, 3010
EcoT14I	CCWWGG	4 cut(s)	471, 489, 786, 1824
EcoT22I	ATGCAT	1 cut(s)	1289
EcoT38I	GRGCYC	1 cut(s)	1374
ErhI	CCWWGG	4 cut(s)	471, 489, 786, 1824
Esp3I	CGTCTC	2 cut(s)	817, 2995
FaqI	GGGAC	2 cut(s)	1855, 2821
FauI	CCCGC	4 cut(s)	302, 1326, 2437, 2846
FbaI	TGATCA	1 cut(s)	2362
FblI	GTMKAC	2 cut(s)	309, 1094
FriOI	GRGCYC	1 cut(s)	1374
FspAI	RTGCGCAY	1 cut(s)	2543
FspBI	CTAG	7 cut(s)	1259, 1358, 1433, 1476, 2120, 2916, 3095
FspI	TGCGCA	1 cut(s)	2543
GlaI	GCGC	2 cut(s)	448, 2543
GsuI	CTGGAG	2 cut(s)	2076, 2085
HaeIII	GGCC	5 cut(s)	63, 84, 541, 1662, 3038
HapII	CCGG	7 cut(s)	81, 409, 523, 587, 1940, 2818, 3083
HhaI	GCGC	2 cut(s)	449, 2544
Hin6I	GCGC	2 cut(s)	447, 2542
HinP1I	GCGC	2 cut(s)	447, 2542
HincII	GTYRAC	2 cut(s)	310, 1095
HindII	GTYRAC	2 cut(s)	310, 1095
HindIII	AAGCTT	1 cut(s)	2409
HpaII	CCGG	7 cut(s)	81, 409, 523, 587, 1940, 2818, 3083
HphI	GGTGA	9 cut(s)	310, 370, 667, 1244, 1300, 1996, 2519, 2648, 2768
Hpy166II	GTNNAC	7 cut(s)	310, 1095, 1447, 1486, 2107, 2173, 2927
Hpy8I	GTNNAC	7 cut(s)	310, 1095, 1447, 1486, 2107, 2173, 2927
Hpy99I	CGWCG	5 cut(s)	44, 71, 311, 1093, 1096
HpyCH4III	ACNGT	3 cut(s)	367, 718, 1392
HpyCH4IV	ACGT	5 cut(s)	1088, 1162, 1279, 1449, 1780
HpyF10VI	GCNNNNNNNGC	6 cut(s)	538, 577, 1511, 1520, 2797, 3071
HpySE526I	ACGT	5 cut(s)	1088, 1162, 1279, 1449, 1780
HspAI	GCGC	2 cut(s)	447, 2542
Kpn2I	TCCGGA	2 cut(s)	408, 522
Ksp22I	TGATCA	1 cut(s)	2362
Kzo9I	GATC	7 cut(s)	43, 917, 1567, 1750, 1927, 2362, 2620
LmnI	GCTCC	5 cut(s)	425, 568, 1377, 1745, 2312
Lsp1109I	GCAGC	9 cut(s)	69, 592, 817, 1303, 1501, 1507, 1529, 2520, 3109
LweI	GCATC	7 cut(s)	306, 519, 652, 853, 1492, 1543, 3139
MaeI	CTAG	7 cut(s)	1259, 1358, 1433, 1476, 2120, 2916, 3095
MaeII	ACGT	5 cut(s)	1088, 1162, 1279, 1449, 1780
MaeIII	GTNAC	8 cut(s)	868, 988, 1376, 1984, 2340, 2554, 2764, 3016
MalI	GATC	7 cut(s)	45, 919, 1569, 1752, 1929, 2364, 2622
MbiI	CCGCTC	1 cut(s)	420
MboI	GATC	7 cut(s)	43, 917, 1567, 1750, 1927, 2362, 2620
MfeI	CAATTG	1 cut(s)	764
MflI	RGATCY	1 cut(s)	2620
MhlI	GDGCHC	3 cut(s)	21, 143, 1374
MluI	ACGCGT	1 cut(s)	898
MlyI	GAGTC	4 cut(s)	93, 820, 947, 3000
MmeI	TCCRAC	3 cut(s)	1060, 1540, 1723
Mph1103I	ATGCAT	1 cut(s)	1289
MroI	TCCGGA	2 cut(s)	408, 522
MseI	TTAA	6 cut(s)	735, 821, 1010, 1830, 2432, 2877
MslI	CAYNNNNRTG	4 cut(s)	555, 1147, 2112, 2593
MspA1I	CMGCKG	2 cut(s)	60, 1520
MspCI	CTTAAG	1 cut(s)	2876
MspI	CCGG	7 cut(s)	81, 409, 523, 587, 1940, 2818, 3083
MspR9I	CCNGG	3 cut(s)	1676, 2818, 3012
MunI	CAATTG	1 cut(s)	764
Mva1269I	GAATGC	2 cut(s)	1471, 3129
MvaI	CCWGG	2 cut(s)	1676, 3012
MvnI	CGCG	3 cut(s)	447, 449, 900
MwoI	GCNNNNNNNGC	6 cut(s)	538, 577, 1511, 1520, 2797, 3071
NciI	CCSGG	1 cut(s)	2818
NcoI	CCATGG	1 cut(s)	471
NdeII	GATC	7 cut(s)	43, 917, 1567, 1750, 1927, 2362, 2620
NheI	GCTAGC	2 cut(s)	1475, 3094
NlaIV	GGNNCC	6 cut(s)	142, 548, 609, 771, 1143, 2837
NmeAIII	GCCGAG	1 cut(s)	567
NmuCI	GTSAC	5 cut(s)	988, 1376, 1984, 2340, 2764
NsbI	TGCGCA	1 cut(s)	2543
NsiI	ATGCAT	1 cut(s)	1289
NspI	RCATGY	5 cut(s)	1269, 1291, 2013, 2542, 2852
NspV	TTCGAA	1 cut(s)	1031
OliI	CACNNNNGTG	1 cut(s)	1147
PaeI	GCATGC	4 cut(s)	1269, 1291, 2542, 2852
PagI	TCATGA	1 cut(s)	2502
PctI	GAATGC	2 cut(s)	1471, 3129
PfeI	GAWTC	9 cut(s)	351, 1411, 1943, 1994, 2066, 2264, 2321, 2826, 3023
PflMI	CCANNNNNTGG	1 cut(s)	472
PleI	GAGTC	4 cut(s)	93, 820, 946, 3000
PpsI	GAGTC	4 cut(s)	93, 820, 946, 3000
Ppu21I	YACGTR	2 cut(s)	1163, 1450
PshAI	GACNNNNGTC	1 cut(s)	2192
Psp124BI	GAGCTC	1 cut(s)	1374
Psp1406I	AACGTT	1 cut(s)	1279
Psp6I	CCWGG	2 cut(s)	1674, 3010
PspGI	CCWGG	2 cut(s)	1674, 3010
PspN4I	GGNNCC	6 cut(s)	142, 548, 609, 771, 1143, 2837
PspPI	GGNCC	6 cut(s)	4, 769, 1316, 1806, 2835, 3036
PstI	CTGCAG	1 cut(s)	1519
PstNI	CAGNNNCTG	3 cut(s)	571, 1523, 1658
PsuI	RGATCY	1 cut(s)	2620
PvuII	CAGCTG	1 cut(s)	1520
RsaI	GTAC	5 cut(s)	518, 1102, 1855, 1897, 2928
RsaNI	GTAC	5 cut(s)	517, 1101, 1854, 1896, 2927
RseI	CAYNNNNRTG	4 cut(s)	555, 1147, 2112, 2593
SacI	GAGCTC	1 cut(s)	1374
SalI	GTCGAC	2 cut(s)	308, 1093
SaqAI	TTAA	6 cut(s)	735, 821, 1010, 1830, 2432, 2877
Sau3AI	GATC	7 cut(s)	43, 917, 1567, 1750, 1927, 2362, 2620
Sau96I	GGNCC	6 cut(s)	4, 769, 1316, 1806, 2835, 3036
ScaI	AGTACT	2 cut(s)	1855, 1897
SchI	GAGTC	4 cut(s)	93, 820, 947, 3000
ScrFI	CCNGG	3 cut(s)	1676, 2818, 3012
SduI	GDGCHC	3 cut(s)	21, 143, 1374
SfaNI	GCATC	7 cut(s)	306, 519, 652, 853, 1492, 1543, 3139
SfcI	CTRYAG	3 cut(s)	1515, 2025, 2683
SfuI	TTCGAA	1 cut(s)	1031
SinI	GGWCC	5 cut(s)	4, 769, 1316, 1806, 2835
SmiMI	CAYNNNNRTG	4 cut(s)	555, 1147, 2112, 2593
SmlI	CTYRAG	1 cut(s)	2876
SmoI	CTYRAG	1 cut(s)	2876
SphI	GCATGC	4 cut(s)	1269, 1291, 2542, 2852
SspMI	CTAG	7 cut(s)	1259, 1358, 1433, 1476, 2120, 2916, 3095
SstI	GAGCTC	1 cut(s)	1374
StyD4I	CCNGG	3 cut(s)	1674, 2816, 3010
StyI	CCWWGG	4 cut(s)	471, 489, 786, 1824
TaaI	ACNGT	3 cut(s)	367, 718, 1392
TaiI	ACGT	5 cut(s)	1091, 1165, 1282, 1452, 1783
TaqI	TCGA	8 cut(s)	39, 309, 916, 941, 1031, 1094, 2231, 2677
TatI	WGTACW	3 cut(s)	1100, 1853, 1895
TauI	GCSGC	4 cut(s)	63, 110, 586, 1665
TfiI	GAWTC	9 cut(s)	351, 1411, 1943, 1994, 2066, 2264, 2321, 2826, 3023
Tru1I	TTAA	6 cut(s)	735, 821, 1010, 1830, 2432, 2877
Tru9I	TTAA	6 cut(s)	735, 821, 1010, 1830, 2432, 2877
TscAI	CASTG	2 cut(s)	1486, 2595
TseFI	GTSAC	5 cut(s)	988, 1376, 1984, 2340, 2764
TseI	GCWGC	9 cut(s)	57, 580, 805, 1291, 1514, 1517, 1520, 2508, 3122
Tsp45I	GTSAC	5 cut(s)	988, 1376, 1984, 2340, 2764
TspGWI	ACGGA	2 cut(s)	407, 1364
TspRI	CASTG	2 cut(s)	1486, 2595
Van91I	CCANNNNNTGG	1 cut(s)	472
Vha464I	CTTAAG	1 cut(s)	2876
VpaK11BI	GGWCC	5 cut(s)	4, 769, 1316, 1806, 2835
XagI	CCTNNNNNAGG	1 cut(s)	1823
XapI	RAATTY	6 cut(s)	633, 910, 1710, 2389, 2425, 2708
XbaI	TCTAGA	2 cut(s)	1432, 2119
XceI	RCATGY	5 cut(s)	1269, 1291, 2013, 2542, 2852
XcmI	CCANNNNNNNNNTGG	1 cut(s)	625
XmiI	GTMKAC	2 cut(s)	309, 1094
XspI	CTAG	7 cut(s)	1259, 1358, 1433, 1476, 2120, 2916, 3095
ZrmI	AGTACT	2 cut(s)	1855, 1897
Zsp2I	ATGCAT	1 cut(s)	1289

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.