MD06G1100400.v1.1

ubiquitin-conjugating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0014896
Physical Location & Seq
Forward (+)
23670618 .. 23681581
10964 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1100400.v1.1.491

Sequence Viewer

Length: 447 bp
ATGGCGTCGAAACGGATCCTGAAGGAGCTGAAGGACCTCCAGAAAGATCCTCCTACTTCGTGCAGCGCTGGCCCAGTTGCAGAAGACATGTTCCACTGGCAGGCAACAATAATGGGTCCTGCTGACAGTCCTTATGCAGGTGGAGTGTTTCTTGTCACTATCCATTTTCCTCCAGACTATCCTTTTAAGCCACCAAAGGTTGCATTCAGGACAAAGGTGTTTCACCCTAATATCAACAGCAACGGTAGCATTTGCCTTGACATATTGAAAGAACAGTGGAGCCCTGCCCTGACCATATCCAAGGTGTTGCTCTCGATCTGTTCACTGTTGACAGACCCAAACCCTGATGACCCTTTGGTCCCGGAGATCGCCCATATGTACAAGACTGACAAGAACAAGTATGAGACCACCGCAAGGAGCTGGACTCAGAAGTATGCTATGGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04013 ko04120 ko04141 ko04624 map04013 map04120 map04141 map04624
Pfam Domains
Protein Families
UQ_con

Protein Analysis

149

Amino Acids

16.52

Weight (kDa)

7.71

Isoelectric Point (pI)

47.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 5 - 141 6.5e-55 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 128
AasI GACNNNNNNGTC 1 cut(s) 356
Acc36I ACCTGC 1 cut(s) 128
AciI CCGC 1 cut(s) 411
AclWI GGATC 3 cut(s) 10, 23, 41
AcuI CTGAAG 2 cut(s) 41, 50
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 1 cut(s) 380
AfeI AGCGCT 1 cut(s) 67
AfiI CCNNNNNNNGG 3 cut(s) 100, 137, 414
AflIII ACRYGT 1 cut(s) 87
AgsI TTSAA 1 cut(s) 268
AluBI AGCT 2 cut(s) 28, 420
AluI AGCT 2 cut(s) 28, 420
Alw26I GTCTC 1 cut(s) 398
AlwI GGATC 3 cut(s) 10, 23, 41
Aor51HI AGCGCT 1 cut(s) 67
AoxI GGCC 1 cut(s) 70
ApeKI GCWGC 1 cut(s) 63
AspLEI GCGC 1 cut(s) 68
AspS9I GGNCC 4 cut(s) 34, 71, 116, 358
AsuC2I CCSGG 1 cut(s) 362
AsuHPI GGTGA 1 cut(s) 215
AvaII GGWCC 3 cut(s) 34, 116, 358
BamHI GGATCC 1 cut(s) 15
BanII GRGCYC 1 cut(s) 284
BbsI GAAGAC 1 cut(s) 90
BbvI GCAGC 1 cut(s) 75
BcnI CCSGG 1 cut(s) 362
BcoDI GTCTC 1 cut(s) 398
BfoI RGCGCY 1 cut(s) 69
BfuAI ACCTGC 1 cut(s) 128
BisI GCNGC 1 cut(s) 64
BlsI GCNGC 1 cut(s) 65
Bme1390I CCNGG 1 cut(s) 362
Bme18I GGWCC 3 cut(s) 34, 116, 358
BmgT120I GGNCC 4 cut(s) 34, 71, 116, 358
BmiI GGNNCC 4 cut(s) 17, 117, 281, 360
BmrFI CCNGG 1 cut(s) 362
BmrI ACTGGG 1 cut(s) 68
BmuI ACTGGG 1 cut(s) 68
BpiI GAAGAC 1 cut(s) 90
BplI GAGNNNNNCTC 2 cut(s) 409, 441
BpmI CTGGAG 2 cut(s) 23, 156
BpuMI CCSGG 1 cut(s) 362
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 398
BsaJI CCNNGG 1 cut(s) 300
Bsc4I CCNNNNNNNGG 3 cut(s) 100, 137, 414
Bse1I ACTGG 2 cut(s) 74, 101
BseDI CCNNGG 1 cut(s) 300
BseLI CCNNNNNNNGG 3 cut(s) 100, 137, 414
BseMII CTCAG 1 cut(s) 440
BseNI ACTGG 2 cut(s) 74, 101
BseXI GCAGC 1 cut(s) 75
BsgI GTGCAG 1 cut(s) 82
BshFI GGCC 1 cut(s) 72
BsiSI CCGG 1 cut(s) 362
BslFI GGGAC 1 cut(s) 344
BslI CCNNNNNNNGG 3 cut(s) 100, 137, 414
BsmAI GTCTC 1 cut(s) 398
BsmFI GGGAC 1 cut(s) 344
BsmI GAATGC 1 cut(s) 203
BsnI GGCC 1 cut(s) 72
Bso31I GGTCTC 1 cut(s) 398
Bsp1286I GDGCHC 1 cut(s) 284
Bsp1407I TGTACA 1 cut(s) 378
Bsp143I GATC 4 cut(s) 15, 46, 315, 366
BspACI CCGC 1 cut(s) 411
BspANI GGCC 1 cut(s) 72
BspCNI CTCAG 1 cut(s) 439
BspLI GGNNCC 4 cut(s) 17, 117, 281, 360
BspMI ACCTGC 1 cut(s) 128
BspPI GGATC 3 cut(s) 10, 23, 41
BspTNI GGTCTC 1 cut(s) 398
BsrGI TGTACA 1 cut(s) 378
BsrI ACTGG 2 cut(s) 74, 101
BssECI CCNNGG 1 cut(s) 300
BssMI GATC 4 cut(s) 15, 46, 315, 366
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 300
Bst4CI ACNGT 4 cut(s) 128, 245, 276, 327
BstACI GRCGYC 1 cut(s) 5
BstAUI TGTACA 1 cut(s) 378
BstC8I GCNNGC 2 cut(s) 70, 102
BstDEI CTNAG 1 cut(s) 426
BstENI CCTNNNNNAGG 1 cut(s) 135
BstH2I RGCGCY 1 cut(s) 69
BstHHI GCGC 1 cut(s) 68
BstKTI GATC 4 cut(s) 18, 49, 318, 369
BstMAI GTCTC 1 cut(s) 398
BstMBI GATC 4 cut(s) 15, 46, 315, 366
BstMWI GCNNNNNNNGC 2 cut(s) 69, 246
BstNSI RCATGY 1 cut(s) 91
BstSCI CCNGG 1 cut(s) 360
BstV1I GCAGC 1 cut(s) 75
BstV2I GAAGAC 1 cut(s) 90
BstX2I RGATCY 2 cut(s) 15, 46
BstYI RGATCY 2 cut(s) 15, 46
BsuRI GGCC 1 cut(s) 72
BtsIMutI CAGTG 3 cut(s) 94, 281, 323
BveI ACCTGC 1 cut(s) 128
Cac8I GCNNGC 2 cut(s) 70, 102
CfoI GCGC 1 cut(s) 68
Cfr13I GGNCC 4 cut(s) 34, 71, 116, 358
Csp6I GTAC 1 cut(s) 379
CviAII CATG 1 cut(s) 88
CviJI RGCY 5 cut(s) 28, 72, 190, 282, 420
CviKI_1 RGCY 5 cut(s) 28, 72, 190, 282, 420
CviQI GTAC 1 cut(s) 379
DdeI CTNAG 1 cut(s) 426
DpnI GATC 4 cut(s) 17, 48, 317, 368
DpnII GATC 4 cut(s) 15, 46, 315, 366
DrdI GACNNNNNNGTC 1 cut(s) 356
DseDI GACNNNNNNGTC 1 cut(s) 356
Eco130I CCWWGG 1 cut(s) 300
Eco24I GRGCYC 1 cut(s) 284
Eco31I GGTCTC 1 cut(s) 398
Eco47I GGWCC 3 cut(s) 34, 116, 358
Eco47III AGCGCT 1 cut(s) 67
Eco57I CTGAAG 2 cut(s) 41, 50
EcoNI CCTNNNNNAGG 1 cut(s) 135
EcoO109I RGGNCCY 2 cut(s) 34, 116
EcoT14I CCWWGG 1 cut(s) 300
EcoT38I GRGCYC 1 cut(s) 284
ErhI CCWWGG 1 cut(s) 300
FaeI CATG 1 cut(s) 91
FaiI YATR 9 cut(s) 89, 135, 263, 296, 375, 377, 402, 435, 440
FaqI GGGAC 1 cut(s) 344
FatI CATG 1 cut(s) 87
FauNDI CATATG 1 cut(s) 375
Fnu4HI GCNGC 1 cut(s) 64
FriOI GRGCYC 1 cut(s) 284
Fsp4HI GCNGC 1 cut(s) 64
GlaI GCGC 1 cut(s) 67
GluI GCNGC 1 cut(s) 64
GsuI CTGGAG 2 cut(s) 23, 156
HaeII RGCGCY 1 cut(s) 69
HaeIII GGCC 1 cut(s) 72
HapII CCGG 1 cut(s) 362
HhaI GCGC 1 cut(s) 68
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 1 cut(s) 91
Hin6I GCGC 1 cut(s) 66
HinP1I GCGC 1 cut(s) 66
HincII GTYRAC 1 cut(s) 330
HindII GTYRAC 1 cut(s) 330
HinfI GANTC 1 cut(s) 424
HpaII CCGG 1 cut(s) 362
HphI GGTGA 1 cut(s) 215
Hpy166II GTNNAC 2 cut(s) 323, 330
Hpy188I TCNGA 1 cut(s) 429
Hpy188III TCNNGA 5 cut(s) 19, 40, 173, 208, 313
Hpy8I GTNNAC 2 cut(s) 323, 330
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 2 cut(s) 16, 25
HpyCH4III ACNGT 4 cut(s) 128, 245, 276, 327
HpyCH4V TGCA 4 cut(s) 63, 80, 137, 203
HpyF10VI GCNNNNNNNGC 2 cut(s) 69, 246
HpyF3I CTNAG 1 cut(s) 426
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 1 cut(s) 91
HspAI GCGC 1 cut(s) 66
Kzo9I GATC 4 cut(s) 15, 46, 315, 366
LmnI GCTCC 3 cut(s) 25, 279, 417
Lsp1109I GCAGC 1 cut(s) 75
MaeIII GTNAC 1 cut(s) 154
MalI GATC 4 cut(s) 17, 48, 317, 368
MboI GATC 4 cut(s) 15, 46, 315, 366
MboII GAAGA 1 cut(s) 95
MflI RGATCY 2 cut(s) 15, 46
MhlI GDGCHC 1 cut(s) 284
MlyI GAGTC 1 cut(s) 418
MnlI CCTC 3 cut(s) 47, 60, 180
MseI TTAA 1 cut(s) 186
MspI CCGG 1 cut(s) 362
MspR9I CCNGG 1 cut(s) 362
Mva1269I GAATGC 1 cut(s) 203
MwoI GCNNNNNNNGC 2 cut(s) 69, 246
NciI CCSGG 1 cut(s) 362
NdeI CATATG 1 cut(s) 375
NdeII GATC 4 cut(s) 15, 46, 315, 366
NlaIII CATG 1 cut(s) 91
NlaIV GGNNCC 4 cut(s) 17, 117, 281, 360
NmuCI GTSAC 1 cut(s) 154
NspI RCATGY 1 cut(s) 91
PaqCI CACCTGC 1 cut(s) 128
PciI ACATGT 1 cut(s) 87
PctI GAATGC 1 cut(s) 203
PfoI TCCNGGA 1 cut(s) 360
PkrI GCNGC 1 cut(s) 65
PleI GAGTC 1 cut(s) 418
PpsI GAGTC 1 cut(s) 418
PpuMI RGGWCCY 2 cut(s) 34, 116
PscI ACATGT 1 cut(s) 87
Psp5II RGGWCCY 2 cut(s) 34, 116
PspN4I GGNNCC 4 cut(s) 17, 117, 281, 360
PspPI GGNCC 4 cut(s) 34, 71, 116, 358
PspPPI RGGWCCY 2 cut(s) 34, 116
PsuI RGATCY 2 cut(s) 15, 46
RsaI GTAC 1 cut(s) 380
RsaNI GTAC 1 cut(s) 379
SaqAI TTAA 1 cut(s) 186
SatI GCNGC 1 cut(s) 64
Sau3AI GATC 4 cut(s) 15, 46, 315, 366
Sau96I GGNCC 4 cut(s) 34, 71, 116, 358
SchI GAGTC 1 cut(s) 418
ScrFI CCNGG 1 cut(s) 362
SduI GDGCHC 1 cut(s) 284
SetI ASST 7 cut(s) 30, 39, 142, 201, 219, 306, 422
SinI GGWCC 3 cut(s) 34, 116, 358
SsiI CCGC 1 cut(s) 411
StyD4I CCNGG 1 cut(s) 360
StyI CCWWGG 1 cut(s) 300
TaaI ACNGT 4 cut(s) 128, 245, 276, 327
TaqI TCGA 2 cut(s) 8, 314
TatI WGTACW 1 cut(s) 378
Tru1I TTAA 1 cut(s) 186
Tru9I TTAA 1 cut(s) 186
TscAI CASTG 3 cut(s) 101, 281, 330
TseFI GTSAC 1 cut(s) 154
TseI GCWGC 1 cut(s) 63
Tsp45I GTSAC 1 cut(s) 154
TspGWI ACGGA 1 cut(s) 28
TspRI CASTG 3 cut(s) 101, 281, 330
VpaK11BI GGWCC 3 cut(s) 34, 116, 358
XagI CCTNNNNNAGG 1 cut(s) 135
XceI RCATGY 1 cut(s) 91
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.