Rh2BG612900

ubiquitin-conjugating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0014896
Physical Location & Seq
Reverse (-)
83502733 .. 83503185
453 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG612900.1

Sequence Viewer

Length: 453 bp
ATGGCGTCCAAGCGGATCTTGAAGGAGCTCAAGGATCTCCAGAAAGATCCCCCTACCTCTTGCAGCGCTGGCCCAGTTGCAGAAGACATGTTTCACTGGCAGGCGACAATCATGGGTCCTGCAGACAGTCCCTACGCAGGTGGAGTGTTTCTTGTCACCATACACTTTCCTCCAGACTATCCTTTTAAGCCACCAAAGGTTGCATTCAGGACAAAGGTGTTTCACCCAAACATTAATAGCAACGGCAGCATTTGCCTTGACATTTTGAAGGAACAGTGGAGTCCTGCGCTGACCATATCGAAGGTATTGCTTTCGATTTGCTCCCTGTTGACAGACCCAAATCCTGATGATCCTTTGGTGCCAGAGATTGCCCATATGTACAAGACCGACAAGAACAAGTATGAGACCACTGAGCTGGACCCAGAAGTACGCCATGGGCTAGTGTGCTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04013 ko04120 ko04141 ko04624 map04013 map04120 map04141 map04624
Pfam Domains
Protein Families
UQ_con

Protein Analysis

150

Amino Acids

16.69

Weight (kDa)

5.91

Isoelectric Point (pI)

47.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 5 - 136 9.5e-54 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 128
Acc36I ACCTGC 1 cut(s) 128
AccB1I GGYRCC 1 cut(s) 358
AciI CCGC 1 cut(s) 13
AclWI GGATC 4 cut(s) 23, 41, 42, 344
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 2 cut(s) 380, 429
AfeI AGCGCT 1 cut(s) 67
AfiI CCNNNNNNNGG 1 cut(s) 137
AflIII ACRYGT 1 cut(s) 87
AgsI TTSAA 2 cut(s) 22, 268
AluBI AGCT 2 cut(s) 28, 415
AluI AGCT 2 cut(s) 28, 415
Alw21I GWGCWC 1 cut(s) 30
Alw26I GTCTC 1 cut(s) 398
AlwI GGATC 4 cut(s) 23, 41, 42, 344
Aor51HI AGCGCT 1 cut(s) 67
AoxI GGCC 1 cut(s) 70
ApeKI GCWGC 3 cut(s) 63, 246, 447
AseI ATTAAT 1 cut(s) 234
AspLEI GCGC 2 cut(s) 68, 289
AspS9I GGNCC 3 cut(s) 71, 116, 418
AsuHPI GGTGA 2 cut(s) 148, 215
AvaII GGWCC 2 cut(s) 116, 418
BanI GGYRCC 1 cut(s) 358
BanII GRGCYC 1 cut(s) 30
BbsI GAAGAC 1 cut(s) 90
Bbv12I GWGCWC 1 cut(s) 30
BbvI GCAGC 3 cut(s) 75, 258, 434
BceAI ACGGC 1 cut(s) 259
BcgI CGANNNNNNTGC 2 cut(s) 289, 323
BcoDI GTCTC 1 cut(s) 398
BfaI CTAG 2 cut(s) 440, 451
BfmI CTRYAG 1 cut(s) 120
BfoI RGCGCY 1 cut(s) 69
BfuAI ACCTGC 1 cut(s) 128
BisI GCNGC 3 cut(s) 64, 247, 448
BlsI GCNGC 3 cut(s) 65, 248, 449
Bme18I GGWCC 2 cut(s) 116, 418
BmgT120I GGNCC 3 cut(s) 71, 116, 418
BmiI GGNNCC 3 cut(s) 117, 360, 420
BmrI ACTGGG 1 cut(s) 68
BmuI ACTGGG 1 cut(s) 68
BpiI GAAGAC 1 cut(s) 90
BpmI CTGGAG 2 cut(s) 23, 156
BpuEI CTTGAG 1 cut(s) 14
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 398
BsaJI CCNNGG 1 cut(s) 433
Bsc4I CCNNNNNNNGG 1 cut(s) 137
Bse1I ACTGG 2 cut(s) 74, 101
BseDI CCNNGG 1 cut(s) 433
BseLI CCNNNNNNNGG 1 cut(s) 137
BseMII CTCAG 1 cut(s) 402
BseNI ACTGG 2 cut(s) 74, 101
BseXI GCAGC 3 cut(s) 75, 258, 434
BshFI GGCC 1 cut(s) 72
BshNI GGYRCC 1 cut(s) 358
BsiHKAI GWGCWC 1 cut(s) 30
BslFI GGGAC 1 cut(s) 114
BslI CCNNNNNNNGG 1 cut(s) 137
BsmAI GTCTC 1 cut(s) 398
BsmFI GGGAC 1 cut(s) 114
BsmI GAATGC 1 cut(s) 203
BsnI GGCC 1 cut(s) 72
Bso31I GGTCTC 1 cut(s) 398
Bsp1286I GDGCHC 1 cut(s) 30
Bsp1407I TGTACA 1 cut(s) 378
Bsp143I GATC 4 cut(s) 15, 34, 46, 349
Bsp19I CCATGG 1 cut(s) 433
BspACI CCGC 1 cut(s) 13
BspANI GGCC 1 cut(s) 72
BspCNI CTCAG 1 cut(s) 403
BspLI GGNNCC 3 cut(s) 117, 360, 420
BspMAI CTGCAG 1 cut(s) 124
BspMI ACCTGC 1 cut(s) 128
BspPI GGATC 4 cut(s) 23, 41, 42, 344
BspT107I GGYRCC 1 cut(s) 358
BspTNI GGTCTC 1 cut(s) 398
BsrGI TGTACA 1 cut(s) 378
BsrI ACTGG 2 cut(s) 74, 101
BssECI CCNNGG 1 cut(s) 433
BssMI GATC 4 cut(s) 15, 34, 46, 349
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 433
Bst4CI ACNGT 2 cut(s) 128, 276
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 1 cut(s) 252
BstAUI TGTACA 1 cut(s) 378
BstC8I GCNNGC 2 cut(s) 70, 102
BstDEI CTNAG 1 cut(s) 411
BstDSI CCRYGG 1 cut(s) 433
BstH2I RGCGCY 1 cut(s) 69
BstHHI GCGC 2 cut(s) 68, 289
BstKTI GATC 4 cut(s) 18, 37, 49, 352
BstMAI GTCTC 1 cut(s) 398
BstMBI GATC 4 cut(s) 15, 34, 46, 349
BstMWI GCNNNNNNNGC 3 cut(s) 69, 246, 252
BstNSI RCATGY 1 cut(s) 91
BstSFI CTRYAG 1 cut(s) 120
BstV1I GCAGC 3 cut(s) 75, 258, 434
BstV2I GAAGAC 1 cut(s) 90
BstX2I RGATCY 3 cut(s) 15, 34, 46
BstXI CCANNNNNNTGG 1 cut(s) 415
BstYI RGATCY 3 cut(s) 15, 34, 46
BsuRI GGCC 1 cut(s) 72
BtgI CCRYGG 1 cut(s) 433
BtsIMutI CAGTG 3 cut(s) 94, 281, 408
BveI ACCTGC 1 cut(s) 128
Cac8I GCNNGC 2 cut(s) 70, 102
CfoI GCGC 2 cut(s) 68, 289
Cfr13I GGNCC 3 cut(s) 71, 116, 418
Csp6I GTAC 2 cut(s) 379, 428
CviAII CATG 3 cut(s) 88, 112, 434
CviJI RGCY 5 cut(s) 28, 72, 190, 415, 439
CviKI_1 RGCY 5 cut(s) 28, 72, 190, 415, 439
CviQI GTAC 2 cut(s) 379, 428
DdeI CTNAG 1 cut(s) 411
DpnI GATC 4 cut(s) 17, 36, 48, 351
DpnII GATC 4 cut(s) 15, 34, 46, 349
Ecl136II GAGCTC 1 cut(s) 28
Eco130I CCWWGG 1 cut(s) 433
Eco24I GRGCYC 1 cut(s) 30
Eco31I GGTCTC 1 cut(s) 398
Eco47I GGWCC 2 cut(s) 116, 418
Eco47III AGCGCT 1 cut(s) 67
Eco53kI GAGCTC 1 cut(s) 28
EcoICRI GAGCTC 1 cut(s) 28
EcoO109I RGGNCCY 1 cut(s) 116
EcoT14I CCWWGG 1 cut(s) 433
EcoT38I GRGCYC 1 cut(s) 30
ErhI CCWWGG 1 cut(s) 433
FaeI CATG 3 cut(s) 91, 115, 437
FaiI YATR 8 cut(s) 89, 113, 161, 296, 375, 377, 402, 435
FalI AAGNNNNNCTT 1 cut(s) 34
FaqI GGGAC 1 cut(s) 114
FatI CATG 3 cut(s) 87, 111, 433
FauNDI CATATG 1 cut(s) 375
Fnu4HI GCNGC 3 cut(s) 64, 247, 448
FriOI GRGCYC 1 cut(s) 30
Fsp4HI GCNGC 3 cut(s) 64, 247, 448
FspBI CTAG 2 cut(s) 440, 451
GlaI GCGC 2 cut(s) 67, 288
GluI GCNGC 3 cut(s) 64, 247, 448
GsuI CTGGAG 2 cut(s) 23, 156
HaeII RGCGCY 1 cut(s) 69
HaeIII GGCC 1 cut(s) 72
HhaI GCGC 2 cut(s) 68, 289
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 3 cut(s) 91, 115, 437
Hin6I GCGC 2 cut(s) 66, 287
HinP1I GCGC 2 cut(s) 66, 287
HincII GTYRAC 1 cut(s) 330
HindII GTYRAC 1 cut(s) 330
HinfI GANTC 1 cut(s) 280
HphI GGTGA 2 cut(s) 148, 215
Hpy166II GTNNAC 1 cut(s) 330
Hpy188III TCNNGA 5 cut(s) 19, 40, 173, 208, 344
Hpy8I GTNNAC 1 cut(s) 330
HpyAV CCTTC 3 cut(s) 16, 262, 295
HpyCH4III ACNGT 2 cut(s) 128, 276
HpyCH4V TGCA 4 cut(s) 63, 80, 122, 203
HpyF10VI GCNNNNNNNGC 3 cut(s) 69, 246, 252
HpyF3I CTNAG 1 cut(s) 411
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 3 cut(s) 91, 115, 437
HspAI GCGC 2 cut(s) 66, 287
Kzo9I GATC 4 cut(s) 15, 34, 46, 349
LmnI GCTCC 2 cut(s) 25, 326
Lsp1109I GCAGC 3 cut(s) 75, 258, 434
MaeI CTAG 2 cut(s) 440, 451
MaeIII GTNAC 1 cut(s) 154
MalI GATC 4 cut(s) 17, 36, 48, 351
MboI GATC 4 cut(s) 15, 34, 46, 349
MboII GAAGA 1 cut(s) 95
MflI RGATCY 3 cut(s) 15, 34, 46
MhlI GDGCHC 1 cut(s) 30
MlyI GAGTC 1 cut(s) 289
MnlI CCTC 2 cut(s) 67, 180
MseI TTAA 2 cut(s) 186, 234
Mva1269I GAATGC 1 cut(s) 203
MwoI GCNNNNNNNGC 3 cut(s) 69, 246, 252
NcoI CCATGG 1 cut(s) 433
NdeI CATATG 1 cut(s) 375
NdeII GATC 4 cut(s) 15, 34, 46, 349
NlaIII CATG 3 cut(s) 91, 115, 437
NlaIV GGNNCC 3 cut(s) 117, 360, 420
NmuCI GTSAC 1 cut(s) 154
NspI RCATGY 1 cut(s) 91
PaqCI CACCTGC 1 cut(s) 128
PciI ACATGT 1 cut(s) 87
PctI GAATGC 1 cut(s) 203
PkrI GCNGC 3 cut(s) 65, 248, 449
PleI GAGTC 1 cut(s) 288
PpsI GAGTC 1 cut(s) 288
PpuMI RGGWCCY 1 cut(s) 116
PscI ACATGT 1 cut(s) 87
PshBI ATTAAT 1 cut(s) 234
Psp124BI GAGCTC 1 cut(s) 30
Psp5II RGGWCCY 1 cut(s) 116
PspN4I GGNNCC 3 cut(s) 117, 360, 420
PspPI GGNCC 3 cut(s) 71, 116, 418
PspPPI RGGWCCY 1 cut(s) 116
PstI CTGCAG 1 cut(s) 124
PsuI RGATCY 3 cut(s) 15, 34, 46
RsaI GTAC 2 cut(s) 380, 429
RsaNI GTAC 2 cut(s) 379, 428
SacI GAGCTC 1 cut(s) 30
SaqAI TTAA 2 cut(s) 186, 234
SatI GCNGC 3 cut(s) 64, 247, 448
Sau3AI GATC 4 cut(s) 15, 34, 46, 349
Sau96I GGNCC 3 cut(s) 71, 116, 418
SchI GAGTC 1 cut(s) 289
SduI GDGCHC 1 cut(s) 30
SetI ASST 7 cut(s) 30, 59, 142, 201, 219, 306, 417
SfcI CTRYAG 1 cut(s) 120
SinI GGWCC 2 cut(s) 116, 418
SmlI CTYRAG 1 cut(s) 29
SmoI CTYRAG 1 cut(s) 29
SsiI CCGC 1 cut(s) 13
SspMI CTAG 2 cut(s) 440, 451
SstI GAGCTC 1 cut(s) 30
StyI CCWWGG 1 cut(s) 433
TaaI ACNGT 2 cut(s) 128, 276
TaqI TCGA 2 cut(s) 299, 314
TaqII GACCGA 1 cut(s) 401
TatI WGTACW 1 cut(s) 378
Tru1I TTAA 2 cut(s) 186, 234
Tru9I TTAA 2 cut(s) 186, 234
TscAI CASTG 3 cut(s) 101, 281, 415
TseFI GTSAC 1 cut(s) 154
TseI GCWGC 3 cut(s) 63, 246, 447
Tsp45I GTSAC 1 cut(s) 154
TspRI CASTG 3 cut(s) 101, 281, 415
VpaK11BI GGWCC 2 cut(s) 116, 418
VspI ATTAAT 1 cut(s) 234
XceI RCATGY 1 cut(s) 91
XspI CTAG 2 cut(s) 440, 451
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.