Rorug07G0024500

ubiquitin-conjugating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0014896
Physical Location & Seq
Reverse (-)
1764654 .. 1765405
752 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0024500.1

Sequence Viewer

Length: 618 bp
ATGGGGCAAATATACACCTTGTATCCAGAGGAGGCCAAACACCCGTTTACCTATCTCGAACAGCTCTGGGAACTCCTTAAATCTAAGGAGATAAACAAGGAGATTATTGAAAAAGTTATTCAGTTCTACCCAAACGTCCCGGGGGCAGCGGAGAAGGAGGAGCAGGATGAAGACGAGAAGTTGACGGCGATGTTAGGGTCGGAGGCCGAAGAGGAGGAGGTTGAGGATCTTCCAGATCCCGAGTGTGATGATTTAGATTTTGAAGACATGAAGAAGAGGGTCATGGAGAGGGCCATGGAGAAGGCAAAGGAGGCAGAACCGACAGAACAAGACATGATGGAGTTGTTGAAACATTGCAGTGAAAACGGATGCAGTTTGAAACCAGGCCAAGACCAGAAGATCCTCCATCTGATGATTTCTTTCTTAAGCAATTCTGATTGTTTTACACTATTCTCATCTGATGAATATGATAGGATTGTCTGCATCAAAATTTGCATGGAAAAGGTCCGAGCCTATCTCGAGAGTTGTTGCATCCTGGACGACTTGGGGACGGTGTACAGCGCCATTGAGACTGTGGAGTCAATAGAAGAAGAAGAAGAAGAAGAAGATTTGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04013 ko04120 ko04141 ko04624 map04013 map04120 map04141 map04624
Pfam Domains
Protein Families
UQ_con

Protein Analysis

205

Amino Acids

23.79

Weight (kDa)

4.22

Isoelectric Point (pI)

63.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 577
AciI CCGC 1 cut(s) 149
AclWI GGATC 3 cut(s) 230, 234, 394
AcsI RAATTY 1 cut(s) 489
AfaI GTAC 1 cut(s) 557
AflII CTTAAG 1 cut(s) 424
AgsI TTSAA 5 cut(s) 110, 263, 349, 379, 613
AjnI CCWGG 2 cut(s) 382, 534
AluBI AGCT 1 cut(s) 64
AluI AGCT 1 cut(s) 64
Alw26I GTCTC 1 cut(s) 563
AlwI GGATC 3 cut(s) 230, 234, 394
Ama87I CYCGRG 3 cut(s) 139, 239, 518
AoxI GGCC 4 cut(s) 33, 204, 291, 385
ApeKI GCWGC 1 cut(s) 146
ApoI RAATTY 1 cut(s) 489
AspLEI GCGC 1 cut(s) 563
AspS9I GGNCC 2 cut(s) 291, 505
AsuC2I CCSGG 2 cut(s) 140, 141
AvaI CYCGRG 3 cut(s) 139, 239, 518
AvaII GGWCC 1 cut(s) 505
BbsI GAAGAC 2 cut(s) 177, 270
BbvI GCAGC 1 cut(s) 158
BccI CCATC 2 cut(s) 331, 414
BceAI ACGGC 1 cut(s) 201
BciT130I CCWGG 2 cut(s) 384, 536
BciVI GTATCC 1 cut(s) 33
BcnI CCSGG 2 cut(s) 140, 141
BcoDI GTCTC 1 cut(s) 563
BfoI RGCGCY 1 cut(s) 564
BfrI CTTAAG 1 cut(s) 424
BfuI GTATCC 1 cut(s) 33
BisI GCNGC 1 cut(s) 147
BlsI GCNGC 1 cut(s) 148
Bme1390I CCNGG 4 cut(s) 140, 141, 384, 536
Bme18I GGWCC 1 cut(s) 505
BmeT110I CYCGRG 3 cut(s) 139, 239, 518
BmgT120I GGNCC 2 cut(s) 291, 505
BmrFI CCNGG 4 cut(s) 140, 141, 384, 536
BmsI GCATC 3 cut(s) 359, 492, 540
BpiI GAAGAC 2 cut(s) 177, 270
BplI GAGNNNNNCTC 2 cut(s) 501, 533
BpuMI CCSGG 2 cut(s) 140, 141
BsaJI CCNNGG 3 cut(s) 139, 140, 294
Bse3DI GCAATG 1 cut(s) 352
BseBI CCWGG 2 cut(s) 384, 536
BseDI CCNNGG 3 cut(s) 139, 140, 294
BseGI GGATG 3 cut(s) 172, 374, 531
BseMI GCAATG 1 cut(s) 352
BseRI GAGGAG 4 cut(s) 44, 173, 227, 230
BseXI GCAGC 1 cut(s) 158
BshFI GGCC 4 cut(s) 35, 206, 293, 387
BsiHKCI CYCGRG 3 cut(s) 139, 239, 518
BsiSI CCGG 1 cut(s) 140
BslFI GGGAC 2 cut(s) 122, 562
BsmAI GTCTC 1 cut(s) 563
BsmFI GGGAC 2 cut(s) 122, 562
BsnI GGCC 4 cut(s) 35, 206, 293, 387
BsoBI CYCGRG 3 cut(s) 139, 239, 518
Bsp1407I TGTACA 1 cut(s) 555
Bsp143I GATC 3 cut(s) 226, 235, 399
Bsp19I CCATGG 1 cut(s) 294
BspACI CCGC 1 cut(s) 149
BspANI GGCC 4 cut(s) 35, 206, 293, 387
BspPI GGATC 3 cut(s) 230, 234, 394
BspTI CTTAAG 1 cut(s) 424
BsrDI GCAATG 1 cut(s) 352
BsrGI TGTACA 1 cut(s) 555
BssECI CCNNGG 3 cut(s) 139, 140, 294
BssMI GATC 3 cut(s) 226, 235, 399
BssT1I CCWWGG 1 cut(s) 294
Bst2UI CCWGG 2 cut(s) 384, 536
Bst4CI ACNGT 2 cut(s) 553, 574
Bst6I CTCTTC 2 cut(s) 204, 269
BstAFI CTTAAG 1 cut(s) 424
BstAUI TGTACA 1 cut(s) 555
BstDEI CTNAG 1 cut(s) 84
BstDSI CCRYGG 1 cut(s) 294
BstF5I GGATG 3 cut(s) 172, 374, 531
BstH2I RGCGCY 1 cut(s) 564
BstHHI GCGC 1 cut(s) 563
BstKTI GATC 3 cut(s) 229, 238, 402
BstMAI GTCTC 1 cut(s) 563
BstMBI GATC 3 cut(s) 226, 235, 399
BstMWI GCNNNNNNNGC 1 cut(s) 311
BstNI CCWGG 2 cut(s) 384, 536
BstSCI CCNGG 4 cut(s) 138, 139, 382, 534
BstV1I GCAGC 1 cut(s) 158
BstV2I GAAGAC 2 cut(s) 177, 270
BstX2I RGATCY 3 cut(s) 226, 235, 399
BstYI RGATCY 3 cut(s) 226, 235, 399
BsuI GTATCC 1 cut(s) 33
BsuRI GGCC 4 cut(s) 35, 206, 293, 387
BtgI CCRYGG 1 cut(s) 294
BtgZI GCGATG 1 cut(s) 203
BtsCI GGATG 3 cut(s) 172, 374, 531
BtsI GCAGTG 1 cut(s) 364
BtsIMutI CAGTG 1 cut(s) 364
CfoI GCGC 1 cut(s) 563
Cfr13I GGNCC 2 cut(s) 291, 505
Cfr9I CCCGGG 1 cut(s) 139
Csp6I GTAC 1 cut(s) 556
CviAII CATG 5 cut(s) 268, 283, 295, 334, 496
CviJI RGCY 6 cut(s) 35, 64, 206, 293, 387, 512
CviKI_1 RGCY 6 cut(s) 35, 64, 206, 293, 387, 512
CviQI GTAC 1 cut(s) 556
DdeI CTNAG 1 cut(s) 84
DpnI GATC 3 cut(s) 228, 237, 401
DpnII GATC 3 cut(s) 226, 235, 399
DrdI GACNNNNNNGTC 1 cut(s) 577
DseDI GACNNNNNNGTC 1 cut(s) 577
Eam1104I CTCTTC 2 cut(s) 204, 269
EarI CTCTTC 2 cut(s) 204, 269
Eco130I CCWWGG 1 cut(s) 294
Eco47I GGWCC 1 cut(s) 505
Eco88I CYCGRG 3 cut(s) 139, 239, 518
EcoRII CCWGG 2 cut(s) 382, 534
EcoT14I CCWWGG 1 cut(s) 294
ErhI CCWWGG 1 cut(s) 294
FaeI CATG 5 cut(s) 271, 286, 298, 337, 499
FaiI YATR 7 cut(s) 13, 269, 284, 296, 335, 468, 497
FaqI GGGAC 2 cut(s) 122, 562
FatI CATG 5 cut(s) 267, 282, 294, 333, 495
Fnu4HI GCNGC 1 cut(s) 147
FokI GGATG 3 cut(s) 179, 381, 518
Fsp4HI GCNGC 1 cut(s) 147
GlaI GCGC 1 cut(s) 562
GluI GCNGC 1 cut(s) 147
HaeII RGCGCY 1 cut(s) 564
HaeIII GGCC 4 cut(s) 35, 206, 293, 387
HapII CCGG 1 cut(s) 140
HhaI GCGC 1 cut(s) 563
Hin1II CATG 5 cut(s) 271, 286, 298, 337, 499
Hin6I GCGC 1 cut(s) 561
HinP1I GCGC 1 cut(s) 561
HincII GTYRAC 1 cut(s) 183
HindII GTYRAC 1 cut(s) 183
HinfI GANTC 1 cut(s) 578
HpaII CCGG 1 cut(s) 140
Hpy166II GTNNAC 3 cut(s) 48, 183, 556
Hpy188I TCNGA 5 cut(s) 202, 411, 436, 460, 509
Hpy188III TCNNGA 6 cut(s) 26, 56, 233, 239, 518, 520
Hpy8I GTNNAC 3 cut(s) 48, 183, 556
HpyAV CCTTC 2 cut(s) 148, 295
HpyCH4III ACNGT 2 cut(s) 553, 574
HpyCH4IV ACGT 1 cut(s) 135
HpyCH4V TGCA 5 cut(s) 357, 372, 483, 495, 531
HpyF10VI GCNNNNNNNGC 1 cut(s) 311
HpyF3I CTNAG 1 cut(s) 84
HpySE526I ACGT 1 cut(s) 135
Hsp92II CATG 5 cut(s) 271, 286, 298, 337, 499
HspAI GCGC 1 cut(s) 561
Kzo9I GATC 3 cut(s) 226, 235, 399
LmnI GCTCC 1 cut(s) 160
Lsp1109I GCAGC 1 cut(s) 158
LweI GCATC 3 cut(s) 359, 492, 540
MaeII ACGT 1 cut(s) 135
MalI GATC 3 cut(s) 228, 237, 401
MboI GATC 3 cut(s) 226, 235, 399
MflI RGATCY 3 cut(s) 226, 235, 399
MluCI AATT 2 cut(s) 430, 489
MlyI GAGTC 1 cut(s) 587
MmeI TCCRAC 1 cut(s) 180
MseI TTAA 2 cut(s) 78, 425
MslI CAYNNNNRTG 1 cut(s) 357
MspA1I CMGCKG 1 cut(s) 149
MspCI CTTAAG 1 cut(s) 424
MspI CCGG 1 cut(s) 140
MspR9I CCNGG 4 cut(s) 140, 141, 384, 536
MvaI CCWGG 2 cut(s) 384, 536
MwoI GCNNNNNNNGC 1 cut(s) 311
NciI CCSGG 2 cut(s) 140, 141
NcoI CCATGG 1 cut(s) 294
NdeII GATC 3 cut(s) 226, 235, 399
NlaIII CATG 5 cut(s) 271, 286, 298, 337, 499
PaeR7I CTCGAG 1 cut(s) 518
PfoI TCCNGGA 1 cut(s) 534
PkrI GCNGC 1 cut(s) 148
PleI GAGTC 1 cut(s) 586
PpsI GAGTC 1 cut(s) 586
Psp6I CCWGG 2 cut(s) 382, 534
PspGI CCWGG 2 cut(s) 382, 534
PspPI GGNCC 2 cut(s) 291, 505
PsuI RGATCY 3 cut(s) 226, 235, 399
RsaI GTAC 1 cut(s) 557
RsaNI GTAC 1 cut(s) 556
RseI CAYNNNNRTG 1 cut(s) 357
SaqAI TTAA 2 cut(s) 78, 425
SatI GCNGC 1 cut(s) 147
Sau3AI GATC 3 cut(s) 226, 235, 399
Sau96I GGNCC 2 cut(s) 291, 505
SchI GAGTC 1 cut(s) 587
ScrFI CCNGG 4 cut(s) 140, 141, 384, 536
SetI ASST 6 cut(s) 20, 53, 66, 138, 222, 507
SfaNI GCATC 3 cut(s) 359, 492, 540
Sfr274I CTCGAG 1 cut(s) 518
SinI GGWCC 1 cut(s) 505
SlaI CTCGAG 1 cut(s) 518
SmaI CCCGGG 1 cut(s) 141
SmiMI CAYNNNNRTG 1 cut(s) 357
SmlI CTYRAG 2 cut(s) 424, 518
SmoI CTYRAG 2 cut(s) 424, 518
Sse9I AATT 2 cut(s) 430, 489
SsiI CCGC 1 cut(s) 149
StyD4I CCNGG 4 cut(s) 138, 139, 382, 534
StyI CCWWGG 1 cut(s) 294
TaaI ACNGT 2 cut(s) 553, 574
TaiI ACGT 1 cut(s) 138
TaqI TCGA 2 cut(s) 57, 519
TasI AATT 2 cut(s) 430, 489
TatI WGTACW 1 cut(s) 555
Tru1I TTAA 2 cut(s) 78, 425
Tru9I TTAA 2 cut(s) 78, 425
TscAI CASTG 1 cut(s) 364
TseI GCWGC 1 cut(s) 146
TspDTI ATGAA 3 cut(s) 183, 284, 477
TspGWI ACGGA 1 cut(s) 381
TspMI CCCGGG 1 cut(s) 139
TspRI CASTG 1 cut(s) 364
Vha464I CTTAAG 1 cut(s) 424
VpaK11BI GGWCC 1 cut(s) 505
XapI RAATTY 1 cut(s) 489
XcmI CCANNNNNNNNNTGG 1 cut(s) 571
XhoI CTCGAG 1 cut(s) 518
XmaI CCCGGG 1 cut(s) 139
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.