pycom01g22500

ubiquitin-conjugating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0014896
Physical Location & Seq
Forward (+)
20066856 .. 20067718
863 bp
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UTR
Exon/CDS
Intron
pycom01g22500.1

Sequence Viewer

Length: 360 bp
ATGTTCCACTGGCAGGCAACAATAATGGGTCCTGCAGATAGTCCATATGCTGGTGGTGTTTTCCTAGTTACTATTCATTTTCCTCCAGATTATCCCTTTAAACCACCAAAGGTGGCATTTAGAACGAAAGTATTCCACCCAAACATAAACAGCAACGGAAGCATTTGCCTTGACATCTTGAAGGAGCAGTGGAGTCCTGCTCTAACTATTTCCAAGGTGTTGCTTTCCATCTGTTCTCTGTTGACGGACCCAAACCCCGATGATCCATTGGTGCCGGAGATTGCTCACATGTACAAGACAGACAGGAATAAATACGAGACGACAGCCAGGAGCTGGACCCAGAAATATGCCATGGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04013 ko04120 ko04141 ko04624 map04013 map04120 map04141 map04624
Pfam Domains
Protein Families
UQ_con

Protein Analysis

120

Amino Acids

13.44

Weight (kDa)

7.8

Isoelectric Point (pI)

43.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 1 - 112 2.1e-48 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 271
AccB7I CCANNNNNTGG 2 cut(s) 50, 333
AclWI GGATC 1 cut(s) 257
AfaI GTAC 1 cut(s) 293
AfiI CCNNNNNNNGG 3 cut(s) 13, 50, 333
AflIII ACRYGT 1 cut(s) 288
AgsI TTSAA 1 cut(s) 181
AjnI CCWGG 1 cut(s) 326
AluBI AGCT 1 cut(s) 333
AluI AGCT 1 cut(s) 333
Alw26I GTCTC 1 cut(s) 311
AlwI GGATC 1 cut(s) 257
AlwNI CAGNNNCTG 1 cut(s) 333
Asp700I GAANNNNTTC 1 cut(s) 131
AspS9I GGNCC 3 cut(s) 29, 247, 336
AvaII GGWCC 3 cut(s) 29, 247, 336
BanI GGYRCC 1 cut(s) 271
BccI CCATC 1 cut(s) 236
BciT130I CCWGG 1 cut(s) 328
BcoDI GTCTC 1 cut(s) 311
BfaI CTAG 1 cut(s) 65
BfmI CTRYAG 1 cut(s) 33
Bme1390I CCNGG 1 cut(s) 328
Bme18I GGWCC 3 cut(s) 29, 247, 336
BmgT120I GGNCC 3 cut(s) 29, 247, 336
BmiI GGNNCC 4 cut(s) 30, 249, 273, 338
BmrFI CCNGG 1 cut(s) 328
BplI GAGNNNNNCTC 2 cut(s) 184, 216
BpmI CTGGAG 1 cut(s) 69
BsaJI CCNNGG 2 cut(s) 213, 351
Bsc4I CCNNNNNNNGG 3 cut(s) 13, 50, 333
Bse1I ACTGG 1 cut(s) 14
BseBI CCWGG 1 cut(s) 328
BseDI CCNNGG 2 cut(s) 213, 351
BseLI CCNNNNNNNGG 3 cut(s) 13, 50, 333
BseNI ACTGG 1 cut(s) 14
BshNI GGYRCC 1 cut(s) 271
BsiSI CCGG 1 cut(s) 275
BslI CCNNNNNNNGG 3 cut(s) 13, 50, 333
BsmAI GTCTC 1 cut(s) 311
BsmBI CGTCTC 1 cut(s) 311
Bsp1407I TGTACA 1 cut(s) 291
Bsp143I GATC 1 cut(s) 262
Bsp19I CCATGG 1 cut(s) 351
BspLI GGNNCC 4 cut(s) 30, 249, 273, 338
BspMAI CTGCAG 1 cut(s) 37
BspPI GGATC 1 cut(s) 257
BspT107I GGYRCC 1 cut(s) 271
BsrGI TGTACA 1 cut(s) 291
BsrI ACTGG 1 cut(s) 14
BssECI CCNNGG 2 cut(s) 213, 351
BssMI GATC 1 cut(s) 262
BssT1I CCWWGG 2 cut(s) 213, 351
Bst2UI CCWGG 1 cut(s) 328
BstAUI TGTACA 1 cut(s) 291
BstC8I GCNNGC 1 cut(s) 15
BstDSI CCRYGG 1 cut(s) 351
BstKTI GATC 1 cut(s) 265
BstMAI GTCTC 1 cut(s) 311
BstMBI GATC 1 cut(s) 262
BstMWI GCNNNNNNNGC 1 cut(s) 159
BstNI CCWGG 1 cut(s) 328
BstNSI RCATGY 1 cut(s) 292
BstSCI CCNGG 1 cut(s) 326
BstSFI CTRYAG 1 cut(s) 33
BtgI CCRYGG 1 cut(s) 351
BtsI GCAGTG 1 cut(s) 194
BtsIMutI CAGTG 2 cut(s) 7, 194
Cac8I GCNNGC 1 cut(s) 15
CaiI CAGNNNCTG 1 cut(s) 333
Cfr13I GGNCC 3 cut(s) 29, 247, 336
Csp6I GTAC 1 cut(s) 292
CviAII CATG 2 cut(s) 289, 352
CviJI RGCY 3 cut(s) 326, 333, 357
CviKI_1 RGCY 3 cut(s) 326, 333, 357
CviQI GTAC 1 cut(s) 292
DpnI GATC 1 cut(s) 264
DpnII GATC 1 cut(s) 262
DraI TTTAAA 1 cut(s) 100
Eco130I CCWWGG 2 cut(s) 213, 351
Eco47I GGWCC 3 cut(s) 29, 247, 336
EcoO109I RGGNCCY 1 cut(s) 29
EcoRII CCWGG 1 cut(s) 326
EcoT14I CCWWGG 2 cut(s) 213, 351
ErhI CCWWGG 2 cut(s) 213, 351
Esp3I CGTCTC 1 cut(s) 311
FaeI CATG 2 cut(s) 292, 355
FaiI YATR 6 cut(s) 46, 48, 146, 290, 348, 353
FatI CATG 2 cut(s) 288, 351
FauNDI CATATG 1 cut(s) 46
FspBI CTAG 1 cut(s) 65
GsuI CTGGAG 1 cut(s) 69
HapII CCGG 1 cut(s) 275
Hin1II CATG 2 cut(s) 292, 355
HincII GTYRAC 1 cut(s) 243
HindII GTYRAC 1 cut(s) 243
HinfI GANTC 1 cut(s) 193
HpaII CCGG 1 cut(s) 275
Hpy166II GTNNAC 1 cut(s) 243
Hpy188III TCNNGA 2 cut(s) 86, 178
Hpy8I GTNNAC 1 cut(s) 243
HpyAV CCTTC 1 cut(s) 175
HpyCH4V TGCA 1 cut(s) 35
HpyF10VI GCNNNNNNNGC 1 cut(s) 159
Hsp92II CATG 2 cut(s) 292, 355
Kzo9I GATC 1 cut(s) 262
LmnI GCTCC 2 cut(s) 184, 330
MaeI CTAG 1 cut(s) 65
MaeIII GTNAC 1 cut(s) 67
MalI GATC 1 cut(s) 264
MboI GATC 1 cut(s) 262
MlyI GAGTC 1 cut(s) 202
MnlI CCTC 1 cut(s) 93
MroXI GAANNNNTTC 1 cut(s) 131
MseI TTAA 1 cut(s) 99
MspI CCGG 1 cut(s) 275
MspR9I CCNGG 1 cut(s) 328
MvaI CCWGG 1 cut(s) 328
MwoI GCNNNNNNNGC 1 cut(s) 159
NcoI CCATGG 1 cut(s) 351
NdeI CATATG 1 cut(s) 46
NdeII GATC 1 cut(s) 262
NlaIII CATG 2 cut(s) 292, 355
NlaIV GGNNCC 4 cut(s) 30, 249, 273, 338
NspI RCATGY 1 cut(s) 292
PciI ACATGT 1 cut(s) 288
PdmI GAANNNNTTC 1 cut(s) 131
PflMI CCANNNNNTGG 2 cut(s) 50, 333
PleI GAGTC 1 cut(s) 201
PpsI GAGTC 1 cut(s) 201
PpuMI RGGWCCY 1 cut(s) 29
PscI ACATGT 1 cut(s) 288
Psp5II RGGWCCY 1 cut(s) 29
Psp6I CCWGG 1 cut(s) 326
PspGI CCWGG 1 cut(s) 326
PspN4I GGNNCC 4 cut(s) 30, 249, 273, 338
PspPI GGNCC 3 cut(s) 29, 247, 336
PspPPI RGGWCCY 1 cut(s) 29
PstI CTGCAG 1 cut(s) 37
PstNI CAGNNNCTG 1 cut(s) 333
RsaI GTAC 1 cut(s) 293
RsaNI GTAC 1 cut(s) 292
SaqAI TTAA 1 cut(s) 99
Sau3AI GATC 1 cut(s) 262
Sau96I GGNCC 3 cut(s) 29, 247, 336
SchI GAGTC 1 cut(s) 202
ScrFI CCNGG 1 cut(s) 328
SetI ASST 3 cut(s) 114, 219, 335
SfcI CTRYAG 1 cut(s) 33
SinI GGWCC 3 cut(s) 29, 247, 336
SspMI CTAG 1 cut(s) 65
StyD4I CCNGG 1 cut(s) 326
StyI CCWWGG 2 cut(s) 213, 351
TatI WGTACW 1 cut(s) 291
Tru1I TTAA 1 cut(s) 99
Tru9I TTAA 1 cut(s) 99
TscAI CASTG 2 cut(s) 14, 194
TspDTI ATGAA 1 cut(s) 65
TspGWI ACGGA 2 cut(s) 171, 260
TspRI CASTG 2 cut(s) 14, 194
Van91I CCANNNNNTGG 2 cut(s) 50, 333
VpaK11BI GGWCC 3 cut(s) 29, 247, 336
XceI RCATGY 1 cut(s) 292
XmnI GAANNNNTTC 1 cut(s) 131
XspI CTAG 1 cut(s) 65
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.