RchiOBHm_Chr2g0167961

ubiquitin-conjugating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0014896
Physical Location & Seq
Reverse (-)
82455203 .. 82455721
519 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ53571

Sequence Viewer

Length: 519 bp
ATGCGTGAGCGAAACCTTTTACACACGCATAGCTGGACTAAACAAAACAGGAGGGTCTTTCAGGTGATGGCGTCCAAGCGGATCTTGAAGGAGCTCAAGGATCTCCAGAAAGATCCCCCTACCTCTTGCAGCGCTGGCCCAGTTGCAGAAGACATGTTTCACTGGCAGGCGACAATCATGGGTCCTGCAGACAGTCCCTACGCAGGTGGAGTGTTTCTTGTCACCATACACTTTCCTCCAGACTATCCTTTTAAGCCACCAAAGGTTGCATTCAGGACAAAGGTGTTTCACCCAAACATTAATAGCAACGGCAGCATTTGCCTTGACATTTTGAAGGAACAGTGGAGTCCTGCGCTGACCATATCGAAGGTATTGCTTTCGATTTGCTCCCTGTTGACAGACCCAAATCCTGATGATCCTTTGGTGCCAGAGATTGCCCATATGTACAAGACCGACAAGAACAAGTATGAGACCACTGAGCTGGACCCAGAAGTACGCCATGGGCTAGTGTGCTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04013 ko04120 ko04141 ko04624 map04013 map04120 map04141 map04624
Pfam Domains
Protein Families
UQ_con

Protein Analysis

172

Amino Acids

19.51

Weight (kDa)

7.69

Isoelectric Point (pI)

45.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 27 - 158 1.5e-53 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 194
Acc36I ACCTGC 1 cut(s) 194
AccB1I GGYRCC 1 cut(s) 424
AciI CCGC 1 cut(s) 79
AclWI GGATC 4 cut(s) 89, 107, 108, 410
AcyI GRCGYC 1 cut(s) 71
AfaI GTAC 2 cut(s) 446, 495
AfeI AGCGCT 1 cut(s) 133
AfiI CCNNNNNNNGG 1 cut(s) 203
AflIII ACRYGT 1 cut(s) 153
AgsI TTSAA 2 cut(s) 88, 334
AluBI AGCT 3 cut(s) 33, 94, 481
AluI AGCT 3 cut(s) 33, 94, 481
Alw21I GWGCWC 1 cut(s) 96
Alw26I GTCTC 1 cut(s) 464
AlwI GGATC 4 cut(s) 89, 107, 108, 410
Aor51HI AGCGCT 1 cut(s) 133
AoxI GGCC 1 cut(s) 136
ApeKI GCWGC 3 cut(s) 129, 312, 513
AseI ATTAAT 1 cut(s) 300
AspLEI GCGC 2 cut(s) 134, 355
AspS9I GGNCC 3 cut(s) 137, 182, 484
AsuHPI GGTGA 3 cut(s) 76, 214, 281
AvaII GGWCC 2 cut(s) 182, 484
BanI GGYRCC 1 cut(s) 424
BanII GRGCYC 1 cut(s) 96
BbsI GAAGAC 1 cut(s) 156
Bbv12I GWGCWC 1 cut(s) 96
BbvI GCAGC 3 cut(s) 141, 324, 500
BccI CCATC 1 cut(s) 61
BceAI ACGGC 1 cut(s) 325
BcgI CGANNNNNNTGC 2 cut(s) 355, 389
BcoDI GTCTC 1 cut(s) 464
BfaI CTAG 2 cut(s) 506, 517
BfmI CTRYAG 1 cut(s) 186
BfoI RGCGCY 1 cut(s) 135
BfuAI ACCTGC 1 cut(s) 194
BisI GCNGC 3 cut(s) 130, 313, 514
BlsI GCNGC 3 cut(s) 131, 314, 515
Bme18I GGWCC 2 cut(s) 182, 484
BmgT120I GGNCC 3 cut(s) 137, 182, 484
BmiI GGNNCC 3 cut(s) 183, 426, 486
BmrI ACTGGG 1 cut(s) 134
BmuI ACTGGG 1 cut(s) 134
BpiI GAAGAC 1 cut(s) 156
BpmI CTGGAG 2 cut(s) 89, 222
BpuEI CTTGAG 1 cut(s) 80
BsaHI GRCGYC 1 cut(s) 71
BsaI GGTCTC 1 cut(s) 464
BsaJI CCNNGG 1 cut(s) 499
Bsc4I CCNNNNNNNGG 1 cut(s) 203
Bse1I ACTGG 2 cut(s) 140, 167
BseDI CCNNGG 1 cut(s) 499
BseLI CCNNNNNNNGG 1 cut(s) 203
BseMII CTCAG 1 cut(s) 468
BseNI ACTGG 2 cut(s) 140, 167
BseXI GCAGC 3 cut(s) 141, 324, 500
BshFI GGCC 1 cut(s) 138
BshNI GGYRCC 1 cut(s) 424
BsiHKAI GWGCWC 1 cut(s) 96
BslFI GGGAC 1 cut(s) 180
BslI CCNNNNNNNGG 1 cut(s) 203
BsmAI GTCTC 1 cut(s) 464
BsmFI GGGAC 1 cut(s) 180
BsmI GAATGC 1 cut(s) 269
BsnI GGCC 1 cut(s) 138
Bso31I GGTCTC 1 cut(s) 464
Bsp1286I GDGCHC 1 cut(s) 96
Bsp1407I TGTACA 1 cut(s) 444
Bsp143I GATC 4 cut(s) 81, 100, 112, 415
Bsp19I CCATGG 1 cut(s) 499
BspACI CCGC 1 cut(s) 79
BspANI GGCC 1 cut(s) 138
BspCNI CTCAG 1 cut(s) 469
BspLI GGNNCC 3 cut(s) 183, 426, 486
BspMAI CTGCAG 1 cut(s) 190
BspMI ACCTGC 1 cut(s) 194
BspPI GGATC 4 cut(s) 89, 107, 108, 410
BspT107I GGYRCC 1 cut(s) 424
BspTNI GGTCTC 1 cut(s) 464
BsrGI TGTACA 1 cut(s) 444
BsrI ACTGG 2 cut(s) 140, 167
BssECI CCNNGG 1 cut(s) 499
BssMI GATC 4 cut(s) 81, 100, 112, 415
BssNI GRCGYC 1 cut(s) 71
BssT1I CCWWGG 1 cut(s) 499
Bst4CI ACNGT 2 cut(s) 194, 342
BstACI GRCGYC 1 cut(s) 71
BstAPI GCANNNNNTGC 1 cut(s) 318
BstAUI TGTACA 1 cut(s) 444
BstC8I GCNNGC 2 cut(s) 136, 168
BstDEI CTNAG 1 cut(s) 477
BstDSI CCRYGG 1 cut(s) 499
BstH2I RGCGCY 1 cut(s) 135
BstHHI GCGC 2 cut(s) 134, 355
BstKTI GATC 4 cut(s) 84, 103, 115, 418
BstMAI GTCTC 1 cut(s) 464
BstMBI GATC 4 cut(s) 81, 100, 112, 415
BstMWI GCNNNNNNNGC 3 cut(s) 135, 312, 318
BstNSI RCATGY 1 cut(s) 157
BstSFI CTRYAG 1 cut(s) 186
BstV1I GCAGC 3 cut(s) 141, 324, 500
BstV2I GAAGAC 1 cut(s) 156
BstX2I RGATCY 3 cut(s) 81, 100, 112
BstXI CCANNNNNNTGG 1 cut(s) 481
BstYI RGATCY 3 cut(s) 81, 100, 112
BsuRI GGCC 1 cut(s) 138
BtgI CCRYGG 1 cut(s) 499
BtsIMutI CAGTG 3 cut(s) 160, 347, 474
BveI ACCTGC 1 cut(s) 194
Cac8I GCNNGC 2 cut(s) 136, 168
CfoI GCGC 2 cut(s) 134, 355
Cfr13I GGNCC 3 cut(s) 137, 182, 484
CseI GACGC 1 cut(s) 60
Csp6I GTAC 2 cut(s) 445, 494
CviAII CATG 3 cut(s) 154, 178, 500
CviJI RGCY 6 cut(s) 33, 94, 138, 256, 481, 505
CviKI_1 RGCY 6 cut(s) 33, 94, 138, 256, 481, 505
CviQI GTAC 2 cut(s) 445, 494
DdeI CTNAG 1 cut(s) 477
DpnI GATC 4 cut(s) 83, 102, 114, 417
DpnII GATC 4 cut(s) 81, 100, 112, 415
Ecl136II GAGCTC 1 cut(s) 94
Eco130I CCWWGG 1 cut(s) 499
Eco24I GRGCYC 1 cut(s) 96
Eco31I GGTCTC 1 cut(s) 464
Eco47I GGWCC 2 cut(s) 182, 484
Eco47III AGCGCT 1 cut(s) 133
Eco53kI GAGCTC 1 cut(s) 94
EcoICRI GAGCTC 1 cut(s) 94
EcoO109I RGGNCCY 1 cut(s) 182
EcoT14I CCWWGG 1 cut(s) 499
EcoT38I GRGCYC 1 cut(s) 96
ErhI CCWWGG 1 cut(s) 499
FaeI CATG 3 cut(s) 157, 181, 503
FaiI YATR 9 cut(s) 30, 155, 179, 227, 362, 441, 443, 468, 501
FalI AAGNNNNNCTT 2 cut(s) 68, 100
FaqI GGGAC 1 cut(s) 180
FatI CATG 3 cut(s) 153, 177, 499
FauNDI CATATG 1 cut(s) 441
Fnu4HI GCNGC 3 cut(s) 130, 313, 514
FriOI GRGCYC 1 cut(s) 96
Fsp4HI GCNGC 3 cut(s) 130, 313, 514
FspBI CTAG 2 cut(s) 506, 517
GlaI GCGC 2 cut(s) 133, 354
GluI GCNGC 3 cut(s) 130, 313, 514
GsuI CTGGAG 2 cut(s) 89, 222
HaeII RGCGCY 1 cut(s) 135
HaeIII GGCC 1 cut(s) 138
HgaI GACGC 1 cut(s) 60
HhaI GCGC 2 cut(s) 134, 355
Hin1I GRCGYC 1 cut(s) 71
Hin1II CATG 3 cut(s) 157, 181, 503
Hin6I GCGC 2 cut(s) 132, 353
HinP1I GCGC 2 cut(s) 132, 353
HincII GTYRAC 1 cut(s) 396
HindII GTYRAC 1 cut(s) 396
HinfI GANTC 1 cut(s) 346
HphI GGTGA 3 cut(s) 76, 214, 281
Hpy166II GTNNAC 1 cut(s) 396
Hpy188III TCNNGA 5 cut(s) 85, 106, 239, 274, 410
Hpy8I GTNNAC 1 cut(s) 396
HpyAV CCTTC 3 cut(s) 82, 328, 361
HpyCH4III ACNGT 2 cut(s) 194, 342
HpyCH4V TGCA 4 cut(s) 129, 146, 188, 269
HpyF10VI GCNNNNNNNGC 3 cut(s) 135, 312, 318
HpyF3I CTNAG 1 cut(s) 477
Hsp92I GRCGYC 1 cut(s) 71
Hsp92II CATG 3 cut(s) 157, 181, 503
HspAI GCGC 2 cut(s) 132, 353
Kzo9I GATC 4 cut(s) 81, 100, 112, 415
LmnI GCTCC 2 cut(s) 91, 392
Lsp1109I GCAGC 3 cut(s) 141, 324, 500
MaeI CTAG 2 cut(s) 506, 517
MaeIII GTNAC 1 cut(s) 220
MalI GATC 4 cut(s) 83, 102, 114, 417
MboI GATC 4 cut(s) 81, 100, 112, 415
MboII GAAGA 1 cut(s) 161
MflI RGATCY 3 cut(s) 81, 100, 112
MhlI GDGCHC 1 cut(s) 96
MlyI GAGTC 1 cut(s) 355
MnlI CCTC 3 cut(s) 45, 133, 246
MseI TTAA 2 cut(s) 252, 300
Mva1269I GAATGC 1 cut(s) 269
MwoI GCNNNNNNNGC 3 cut(s) 135, 312, 318
NcoI CCATGG 1 cut(s) 499
NdeI CATATG 1 cut(s) 441
NdeII GATC 4 cut(s) 81, 100, 112, 415
NlaIII CATG 3 cut(s) 157, 181, 503
NlaIV GGNNCC 3 cut(s) 183, 426, 486
NmuCI GTSAC 1 cut(s) 220
NspI RCATGY 1 cut(s) 157
PaqCI CACCTGC 1 cut(s) 194
PciI ACATGT 1 cut(s) 153
PctI GAATGC 1 cut(s) 269
PkrI GCNGC 3 cut(s) 131, 314, 515
PleI GAGTC 1 cut(s) 354
PpsI GAGTC 1 cut(s) 354
PpuMI RGGWCCY 1 cut(s) 182
PscI ACATGT 1 cut(s) 153
PshBI ATTAAT 1 cut(s) 300
Psp124BI GAGCTC 1 cut(s) 96
Psp5II RGGWCCY 1 cut(s) 182
PspN4I GGNNCC 3 cut(s) 183, 426, 486
PspPI GGNCC 3 cut(s) 137, 182, 484
PspPPI RGGWCCY 1 cut(s) 182
PstI CTGCAG 1 cut(s) 190
PsuI RGATCY 3 cut(s) 81, 100, 112
RsaI GTAC 2 cut(s) 446, 495
RsaNI GTAC 2 cut(s) 445, 494
SacI GAGCTC 1 cut(s) 96
SaqAI TTAA 2 cut(s) 252, 300
SatI GCNGC 3 cut(s) 130, 313, 514
Sau3AI GATC 4 cut(s) 81, 100, 112, 415
Sau96I GGNCC 3 cut(s) 137, 182, 484
SchI GAGTC 1 cut(s) 355
SduI GDGCHC 1 cut(s) 96
SfcI CTRYAG 1 cut(s) 186
SinI GGWCC 2 cut(s) 182, 484
SmlI CTYRAG 1 cut(s) 95
SmoI CTYRAG 1 cut(s) 95
SsiI CCGC 1 cut(s) 79
SspMI CTAG 2 cut(s) 506, 517
SstI GAGCTC 1 cut(s) 96
StyI CCWWGG 1 cut(s) 499
TaaI ACNGT 2 cut(s) 194, 342
TaqI TCGA 2 cut(s) 365, 380
TaqII GACCGA 1 cut(s) 467
TatI WGTACW 1 cut(s) 444
Tru1I TTAA 2 cut(s) 252, 300
Tru9I TTAA 2 cut(s) 252, 300
TscAI CASTG 3 cut(s) 167, 347, 481
TseFI GTSAC 1 cut(s) 220
TseI GCWGC 3 cut(s) 129, 312, 513
Tsp45I GTSAC 1 cut(s) 220
TspRI CASTG 3 cut(s) 167, 347, 481
VpaK11BI GGWCC 2 cut(s) 182, 484
VspI ATTAAT 1 cut(s) 300
XceI RCATGY 1 cut(s) 157
XspI CTAG 2 cut(s) 506, 517
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.