MD14G1119900.v1.1

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0014896
Physical Location & Seq
Forward (+)
19409177 .. 19413244
4068 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1119900.v1.1.491

Sequence Viewer

Length: 465 bp
ATGGCGTCGAAACGGATCCTGAAGGAGCTCAAGGACCTCCAGAAAGATCCTCCTACTTCTTGCAGCGCTGTTATTGCATGGTTCCTTGGTCCAGTTGCAGAAGACATGTTCCACTGGCAGGCAACAATAATGGGTCCAGCTGACAGTCCTTATGCAGGTGGAGTGTTTCTTGTCACTATCCATTTTCCTCCAGACTATCCTTTCAAGCCACCCAAGGTTGCATTCAGGACAAAGGTGTTTCACCCTAATATCAACAGCAACGGTAGCATTTGCCTTGACATATTGAAAGAACAGTGGAGCCCTGCCCTGACCATATCCAAGGTGTTGCTTTCGATCTGTTCATTGTTGACAGACCCAAACCCTGATGATCCTTTGGTGCCGGAGATTGCCCACATGTACAAGACTGACAAGAACAAGTATGAGACCACCGCAAGGAGCTGGACTCAGAAGTATGCTATGGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04013 ko04120 ko04141 ko04624 map04013 map04120 map04141 map04624
Pfam Domains
Protein Families
UQ_con

Protein Analysis

155

Amino Acids

17.25

Weight (kDa)

7.71

Isoelectric Point (pI)

46.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 5 - 147 3.9e-52 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 146
Acc36I ACCTGC 1 cut(s) 146
AccB1I GGYRCC 1 cut(s) 376
AciI CCGC 1 cut(s) 429
AclWI GGATC 4 cut(s) 10, 23, 41, 362
AcuI CTGAAG 1 cut(s) 41
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 1 cut(s) 398
AfeI AGCGCT 1 cut(s) 67
AfiI CCNNNNNNNGG 3 cut(s) 118, 155, 432
AflIII ACRYGT 2 cut(s) 105, 393
AgsI TTSAA 2 cut(s) 205, 286
AjuI GAANNNNNNNTTGG 2 cut(s) 206, 238
AluBI AGCT 3 cut(s) 28, 140, 438
AluI AGCT 3 cut(s) 28, 140, 438
Alw21I GWGCWC 1 cut(s) 30
Alw26I GTCTC 1 cut(s) 416
AlwI GGATC 4 cut(s) 10, 23, 41, 362
Aor51HI AGCGCT 1 cut(s) 67
ApeKI GCWGC 1 cut(s) 63
AspLEI GCGC 1 cut(s) 68
AspS9I GGNCC 3 cut(s) 34, 89, 134
AsuHPI GGTGA 1 cut(s) 233
AvaII GGWCC 3 cut(s) 34, 89, 134
BamHI GGATCC 1 cut(s) 15
BanI GGYRCC 1 cut(s) 376
BanII GRGCYC 2 cut(s) 30, 302
BbsI GAAGAC 1 cut(s) 108
Bbv12I GWGCWC 1 cut(s) 30
BbvI GCAGC 1 cut(s) 75
BcoDI GTCTC 1 cut(s) 416
BfoI RGCGCY 1 cut(s) 69
BfuAI ACCTGC 1 cut(s) 146
BisI GCNGC 1 cut(s) 64
BlsI GCNGC 1 cut(s) 65
Bme18I GGWCC 3 cut(s) 34, 89, 134
BmgT120I GGNCC 3 cut(s) 34, 89, 134
BmiI GGNNCC 5 cut(s) 17, 83, 135, 299, 378
BpiI GAAGAC 1 cut(s) 108
BplI GAGNNNNNCTC 2 cut(s) 427, 459
BpmI CTGGAG 2 cut(s) 23, 174
BpuEI CTTGAG 1 cut(s) 14
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 416
BsaJI CCNNGG 3 cut(s) 85, 213, 318
Bsc4I CCNNNNNNNGG 3 cut(s) 118, 155, 432
Bse1I ACTGG 2 cut(s) 92, 119
BseDI CCNNGG 3 cut(s) 85, 213, 318
BseLI CCNNNNNNNGG 3 cut(s) 118, 155, 432
BseMII CTCAG 1 cut(s) 458
BseNI ACTGG 2 cut(s) 92, 119
BseXI GCAGC 1 cut(s) 75
BshNI GGYRCC 1 cut(s) 376
BsiHKAI GWGCWC 1 cut(s) 30
BsiSI CCGG 1 cut(s) 380
BslI CCNNNNNNNGG 3 cut(s) 118, 155, 432
BsmAI GTCTC 1 cut(s) 416
BsmI GAATGC 1 cut(s) 221
Bso31I GGTCTC 1 cut(s) 416
Bsp1286I GDGCHC 2 cut(s) 30, 302
Bsp1407I TGTACA 1 cut(s) 396
Bsp143I GATC 4 cut(s) 15, 46, 333, 367
BspACI CCGC 1 cut(s) 429
BspCNI CTCAG 1 cut(s) 457
BspLI GGNNCC 5 cut(s) 17, 83, 135, 299, 378
BspMI ACCTGC 1 cut(s) 146
BspPI GGATC 4 cut(s) 10, 23, 41, 362
BspT107I GGYRCC 1 cut(s) 376
BspTNI GGTCTC 1 cut(s) 416
BsrGI TGTACA 1 cut(s) 396
BsrI ACTGG 2 cut(s) 92, 119
BssECI CCNNGG 3 cut(s) 85, 213, 318
BssMI GATC 4 cut(s) 15, 46, 333, 367
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 3 cut(s) 85, 213, 318
Bst4CI ACNGT 3 cut(s) 146, 263, 294
BstACI GRCGYC 1 cut(s) 5
BstAUI TGTACA 1 cut(s) 396
BstC8I GCNNGC 1 cut(s) 120
BstDEI CTNAG 1 cut(s) 444
BstENI CCTNNNNNAGG 1 cut(s) 153
BstH2I RGCGCY 1 cut(s) 69
BstHHI GCGC 1 cut(s) 68
BstKTI GATC 4 cut(s) 18, 49, 336, 370
BstMAI GTCTC 1 cut(s) 416
BstMBI GATC 4 cut(s) 15, 46, 333, 367
BstMWI GCNNNNNNNGC 2 cut(s) 74, 264
BstNSI RCATGY 2 cut(s) 109, 397
BstV1I GCAGC 1 cut(s) 75
BstV2I GAAGAC 1 cut(s) 108
BstX2I RGATCY 2 cut(s) 15, 46
BstYI RGATCY 2 cut(s) 15, 46
BtsIMutI CAGTG 2 cut(s) 112, 299
BveI ACCTGC 1 cut(s) 146
Cac8I GCNNGC 1 cut(s) 120
CfoI GCGC 1 cut(s) 68
Cfr13I GGNCC 3 cut(s) 34, 89, 134
Csp6I GTAC 1 cut(s) 397
CviAII CATG 3 cut(s) 78, 106, 394
CviJI RGCY 5 cut(s) 28, 140, 208, 300, 438
CviKI_1 RGCY 5 cut(s) 28, 140, 208, 300, 438
CviQI GTAC 1 cut(s) 397
DdeI CTNAG 1 cut(s) 444
DpnI GATC 4 cut(s) 17, 48, 335, 369
DpnII GATC 4 cut(s) 15, 46, 333, 367
Ecl136II GAGCTC 1 cut(s) 28
Eco130I CCWWGG 3 cut(s) 85, 213, 318
Eco24I GRGCYC 2 cut(s) 30, 302
Eco31I GGTCTC 1 cut(s) 416
Eco47I GGWCC 3 cut(s) 34, 89, 134
Eco47III AGCGCT 1 cut(s) 67
Eco53kI GAGCTC 1 cut(s) 28
Eco57I CTGAAG 1 cut(s) 41
EcoICRI GAGCTC 1 cut(s) 28
EcoNI CCTNNNNNAGG 1 cut(s) 153
EcoO109I RGGNCCY 1 cut(s) 34
EcoT14I CCWWGG 3 cut(s) 85, 213, 318
EcoT38I GRGCYC 2 cut(s) 30, 302
ErhI CCWWGG 3 cut(s) 85, 213, 318
FaeI CATG 3 cut(s) 81, 109, 397
FaiI YATR 9 cut(s) 79, 107, 153, 281, 314, 395, 420, 453, 458
FatI CATG 3 cut(s) 77, 105, 393
Fnu4HI GCNGC 1 cut(s) 64
FriOI GRGCYC 2 cut(s) 30, 302
Fsp4HI GCNGC 1 cut(s) 64
GlaI GCGC 1 cut(s) 67
GluI GCNGC 1 cut(s) 64
GsuI CTGGAG 2 cut(s) 23, 174
HaeII RGCGCY 1 cut(s) 69
HapII CCGG 1 cut(s) 380
HhaI GCGC 1 cut(s) 68
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 3 cut(s) 81, 109, 397
Hin6I GCGC 1 cut(s) 66
HinP1I GCGC 1 cut(s) 66
HincII GTYRAC 1 cut(s) 348
HindII GTYRAC 1 cut(s) 348
HinfI GANTC 1 cut(s) 442
HpaII CCGG 1 cut(s) 380
HphI GGTGA 1 cut(s) 233
Hpy166II GTNNAC 1 cut(s) 348
Hpy188I TCNGA 1 cut(s) 447
Hpy188III TCNNGA 4 cut(s) 19, 40, 191, 226
Hpy8I GTNNAC 1 cut(s) 348
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 1 cut(s) 16
HpyCH4III ACNGT 3 cut(s) 146, 263, 294
HpyCH4V TGCA 5 cut(s) 63, 77, 98, 155, 221
HpyF10VI GCNNNNNNNGC 2 cut(s) 74, 264
HpyF3I CTNAG 1 cut(s) 444
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 3 cut(s) 81, 109, 397
HspAI GCGC 1 cut(s) 66
Kzo9I GATC 4 cut(s) 15, 46, 333, 367
LmnI GCTCC 3 cut(s) 25, 297, 435
Lsp1109I GCAGC 1 cut(s) 75
MaeIII GTNAC 1 cut(s) 172
MalI GATC 4 cut(s) 17, 48, 335, 369
MboI GATC 4 cut(s) 15, 46, 333, 367
MboII GAAGA 1 cut(s) 113
MflI RGATCY 2 cut(s) 15, 46
MhlI GDGCHC 2 cut(s) 30, 302
MlyI GAGTC 1 cut(s) 436
MnlI CCTC 3 cut(s) 47, 60, 198
MspA1I CMGCKG 1 cut(s) 140
MspI CCGG 1 cut(s) 380
Mva1269I GAATGC 1 cut(s) 221
MwoI GCNNNNNNNGC 2 cut(s) 74, 264
NdeII GATC 4 cut(s) 15, 46, 333, 367
NlaIII CATG 3 cut(s) 81, 109, 397
NlaIV GGNNCC 5 cut(s) 17, 83, 135, 299, 378
NmuCI GTSAC 1 cut(s) 172
NspI RCATGY 2 cut(s) 109, 397
PaqCI CACCTGC 1 cut(s) 146
PciI ACATGT 2 cut(s) 105, 393
PctI GAATGC 1 cut(s) 221
PkrI GCNGC 1 cut(s) 65
PleI GAGTC 1 cut(s) 436
PpsI GAGTC 1 cut(s) 436
PpuMI RGGWCCY 1 cut(s) 34
PscI ACATGT 2 cut(s) 105, 393
Psp124BI GAGCTC 1 cut(s) 30
Psp5II RGGWCCY 1 cut(s) 34
PspN4I GGNNCC 5 cut(s) 17, 83, 135, 299, 378
PspPI GGNCC 3 cut(s) 34, 89, 134
PspPPI RGGWCCY 1 cut(s) 34
PsuI RGATCY 2 cut(s) 15, 46
PvuII CAGCTG 1 cut(s) 140
RsaI GTAC 1 cut(s) 398
RsaNI GTAC 1 cut(s) 397
SacI GAGCTC 1 cut(s) 30
SatI GCNGC 1 cut(s) 64
Sau3AI GATC 4 cut(s) 15, 46, 333, 367
Sau96I GGNCC 3 cut(s) 34, 89, 134
SchI GAGTC 1 cut(s) 436
SduI GDGCHC 2 cut(s) 30, 302
SetI ASST 8 cut(s) 30, 39, 142, 160, 219, 237, 324, 440
SinI GGWCC 3 cut(s) 34, 89, 134
SmlI CTYRAG 1 cut(s) 29
SmoI CTYRAG 1 cut(s) 29
SsiI CCGC 1 cut(s) 429
SstI GAGCTC 1 cut(s) 30
StyI CCWWGG 3 cut(s) 85, 213, 318
TaaI ACNGT 3 cut(s) 146, 263, 294
TaqI TCGA 2 cut(s) 8, 332
TatI WGTACW 1 cut(s) 396
TscAI CASTG 2 cut(s) 119, 299
TseFI GTSAC 1 cut(s) 172
TseI GCWGC 1 cut(s) 63
Tsp45I GTSAC 1 cut(s) 172
TspDTI ATGAA 1 cut(s) 330
TspGWI ACGGA 1 cut(s) 28
TspRI CASTG 2 cut(s) 119, 299
VpaK11BI GGWCC 3 cut(s) 34, 89, 134
XagI CCTNNNNNAGG 1 cut(s) 153
XceI RCATGY 2 cut(s) 109, 397
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.