RchiOBHm_Chr2g0168071

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0014896
Physical Location & Seq
Forward (+)
82534889 .. 82535599
711 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ53579

Sequence Viewer

Length: 513 bp
ATGCGTGAGCGAAACCTTTTACACACGCATAGCTGGACTAAACAAAACAGGATGGTCTTTCAGGTGATGGCGTCCAAGCGGATCTTGAAGGAGCTCAAGGATCTCCAGAAAGATCCTCCTACCTCTTGCAGCGCTGGCCCAGTTGCAGAAGACATGTTTCACTGGCAGGCGACAATCATGGGTCCTGCAGACGGTCCCTACGCAGGTGGAGTGTTTCTTGTCACCATACACTTTCCTCCAGACTATCCTTTTAAGCCACCAAAGGTTGCATTCAGGACAAAGGTGTTTCACCCAAACATTAATAGCAATGGCAGCATTTGCCTTGACATTTTGAAGGAACAGTGGAGTCCTGCGCTGACCATATCGAAGGTATTGCTTTCGATTTGCTCCCTGTTGACAGACCCAAATCCTGATGATCCTTTGGTGCCAGAGATTGCCCATATGTACAAGACCGACAAGAACAAGTATGAGACCACTGCAAGGAGCTGGACCCAGAAGTACGCCATGGGCTAG

Protein Analysis

170

Amino Acids

19.29

Weight (kDa)

8.96

Isoelectric Point (pI)

38.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 27 - 163 2.2e-54 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 194
Acc36I ACCTGC 1 cut(s) 194
AccB1I GGYRCC 1 cut(s) 424
AciI CCGC 1 cut(s) 79
AclWI GGATC 4 cut(s) 89, 107, 108, 410
AcyI GRCGYC 1 cut(s) 71
AfaI GTAC 2 cut(s) 446, 500
AfeI AGCGCT 1 cut(s) 133
AfiI CCNNNNNNNGG 3 cut(s) 191, 203, 480
AflIII ACRYGT 1 cut(s) 153
AgsI TTSAA 2 cut(s) 88, 334
AluBI AGCT 3 cut(s) 33, 94, 486
AluI AGCT 3 cut(s) 33, 94, 486
Alw21I GWGCWC 1 cut(s) 96
Alw26I GTCTC 1 cut(s) 464
AlwI GGATC 4 cut(s) 89, 107, 108, 410
Aor51HI AGCGCT 1 cut(s) 133
AoxI GGCC 1 cut(s) 136
ApeKI GCWGC 2 cut(s) 129, 312
AseI ATTAAT 1 cut(s) 300
AspLEI GCGC 2 cut(s) 134, 355
AspS9I GGNCC 4 cut(s) 137, 182, 194, 489
AsuHPI GGTGA 3 cut(s) 76, 214, 281
AvaII GGWCC 3 cut(s) 182, 194, 489
BanI GGYRCC 1 cut(s) 424
BanII GRGCYC 1 cut(s) 96
BbsI GAAGAC 1 cut(s) 156
Bbv12I GWGCWC 1 cut(s) 96
BbvI GCAGC 2 cut(s) 141, 324
BccI CCATC 2 cut(s) 46, 61
BcgI CGANNNNNNTGC 2 cut(s) 355, 389
BcoDI GTCTC 1 cut(s) 464
BfaI CTAG 1 cut(s) 511
BfmI CTRYAG 1 cut(s) 186
BfoI RGCGCY 1 cut(s) 135
BfuAI ACCTGC 1 cut(s) 194
BisI GCNGC 2 cut(s) 130, 313
BlsI GCNGC 2 cut(s) 131, 314
Bme18I GGWCC 3 cut(s) 182, 194, 489
BmgT120I GGNCC 4 cut(s) 137, 182, 194, 489
BmiI GGNNCC 4 cut(s) 183, 196, 426, 491
BmrI ACTGGG 1 cut(s) 134
BmuI ACTGGG 1 cut(s) 134
BpiI GAAGAC 1 cut(s) 156
BpmI CTGGAG 2 cut(s) 89, 222
BpuEI CTTGAG 1 cut(s) 80
BsaHI GRCGYC 1 cut(s) 71
BsaI GGTCTC 1 cut(s) 464
BsaJI CCNNGG 1 cut(s) 504
Bsc4I CCNNNNNNNGG 3 cut(s) 191, 203, 480
Bse1I ACTGG 2 cut(s) 140, 167
Bse3DI GCAATG 1 cut(s) 313
BseDI CCNNGG 1 cut(s) 504
BseGI GGATG 1 cut(s) 57
BseLI CCNNNNNNNGG 3 cut(s) 191, 203, 480
BseMI GCAATG 1 cut(s) 313
BseNI ACTGG 2 cut(s) 140, 167
BseXI GCAGC 2 cut(s) 141, 324
BshFI GGCC 1 cut(s) 138
BshNI GGYRCC 1 cut(s) 424
BsiHKAI GWGCWC 1 cut(s) 96
BslFI GGGAC 1 cut(s) 180
BslI CCNNNNNNNGG 3 cut(s) 191, 203, 480
BsmAI GTCTC 1 cut(s) 464
BsmFI GGGAC 1 cut(s) 180
BsmI GAATGC 1 cut(s) 269
BsnI GGCC 1 cut(s) 138
Bso31I GGTCTC 1 cut(s) 464
Bsp1286I GDGCHC 1 cut(s) 96
Bsp1407I TGTACA 1 cut(s) 444
Bsp143I GATC 4 cut(s) 81, 100, 112, 415
Bsp19I CCATGG 1 cut(s) 504
BspACI CCGC 1 cut(s) 79
BspANI GGCC 1 cut(s) 138
BspLI GGNNCC 4 cut(s) 183, 196, 426, 491
BspMAI CTGCAG 1 cut(s) 190
BspMI ACCTGC 1 cut(s) 194
BspPI GGATC 4 cut(s) 89, 107, 108, 410
BspT107I GGYRCC 1 cut(s) 424
BspTNI GGTCTC 1 cut(s) 464
BsrDI GCAATG 1 cut(s) 313
BsrGI TGTACA 1 cut(s) 444
BsrI ACTGG 2 cut(s) 140, 167
BssECI CCNNGG 1 cut(s) 504
BssMI GATC 4 cut(s) 81, 100, 112, 415
BssNI GRCGYC 1 cut(s) 71
BssT1I CCWWGG 1 cut(s) 504
Bst4CI ACNGT 2 cut(s) 194, 342
BstACI GRCGYC 1 cut(s) 71
BstAPI GCANNNNNTGC 1 cut(s) 318
BstAUI TGTACA 1 cut(s) 444
BstC8I GCNNGC 2 cut(s) 136, 168
BstDSI CCRYGG 1 cut(s) 504
BstF5I GGATG 1 cut(s) 57
BstH2I RGCGCY 1 cut(s) 135
BstHHI GCGC 2 cut(s) 134, 355
BstKTI GATC 4 cut(s) 84, 103, 115, 418
BstMAI GTCTC 1 cut(s) 464
BstMBI GATC 4 cut(s) 81, 100, 112, 415
BstMWI GCNNNNNNNGC 3 cut(s) 135, 312, 318
BstNSI RCATGY 1 cut(s) 157
BstSFI CTRYAG 1 cut(s) 186
BstV1I GCAGC 2 cut(s) 141, 324
BstV2I GAAGAC 1 cut(s) 156
BstX2I RGATCY 3 cut(s) 81, 100, 112
BstYI RGATCY 3 cut(s) 81, 100, 112
BsuRI GGCC 1 cut(s) 138
BtgI CCRYGG 1 cut(s) 504
BtsCI GGATG 1 cut(s) 57
BtsI GCAGTG 1 cut(s) 474
BtsIMutI CAGTG 3 cut(s) 160, 347, 474
BveI ACCTGC 1 cut(s) 194
Cac8I GCNNGC 2 cut(s) 136, 168
CfoI GCGC 2 cut(s) 134, 355
Cfr13I GGNCC 4 cut(s) 137, 182, 194, 489
CseI GACGC 1 cut(s) 60
Csp6I GTAC 2 cut(s) 445, 499
CviAII CATG 3 cut(s) 154, 178, 505
CviJI RGCY 6 cut(s) 33, 94, 138, 256, 486, 510
CviKI_1 RGCY 6 cut(s) 33, 94, 138, 256, 486, 510
CviQI GTAC 2 cut(s) 445, 499
DpnI GATC 4 cut(s) 83, 102, 114, 417
DpnII GATC 4 cut(s) 81, 100, 112, 415
Ecl136II GAGCTC 1 cut(s) 94
Eco130I CCWWGG 1 cut(s) 504
Eco24I GRGCYC 1 cut(s) 96
Eco31I GGTCTC 1 cut(s) 464
Eco47I GGWCC 3 cut(s) 182, 194, 489
Eco47III AGCGCT 1 cut(s) 133
Eco53kI GAGCTC 1 cut(s) 94
EcoICRI GAGCTC 1 cut(s) 94
EcoO109I RGGNCCY 1 cut(s) 182
EcoT14I CCWWGG 1 cut(s) 504
EcoT38I GRGCYC 1 cut(s) 96
ErhI CCWWGG 1 cut(s) 504
FaeI CATG 3 cut(s) 157, 181, 508
FaiI YATR 9 cut(s) 30, 155, 179, 227, 362, 441, 443, 468, 506
FalI AAGNNNNNCTT 2 cut(s) 68, 100
FaqI GGGAC 1 cut(s) 180
FatI CATG 3 cut(s) 153, 177, 504
FauNDI CATATG 1 cut(s) 441
Fnu4HI GCNGC 2 cut(s) 130, 313
FokI GGATG 1 cut(s) 64
FriOI GRGCYC 1 cut(s) 96
Fsp4HI GCNGC 2 cut(s) 130, 313
FspBI CTAG 1 cut(s) 511
GlaI GCGC 2 cut(s) 133, 354
GluI GCNGC 2 cut(s) 130, 313
GsuI CTGGAG 2 cut(s) 89, 222
HaeII RGCGCY 1 cut(s) 135
HaeIII GGCC 1 cut(s) 138
HgaI GACGC 1 cut(s) 60
HhaI GCGC 2 cut(s) 134, 355
Hin1I GRCGYC 1 cut(s) 71
Hin1II CATG 3 cut(s) 157, 181, 508
Hin6I GCGC 2 cut(s) 132, 353
HinP1I GCGC 2 cut(s) 132, 353
HincII GTYRAC 1 cut(s) 396
HindII GTYRAC 1 cut(s) 396
HinfI GANTC 1 cut(s) 346
HphI GGTGA 3 cut(s) 76, 214, 281
Hpy166II GTNNAC 1 cut(s) 396
Hpy188III TCNNGA 5 cut(s) 85, 106, 239, 274, 410
Hpy8I GTNNAC 1 cut(s) 396
HpyAV CCTTC 3 cut(s) 82, 328, 361
HpyCH4III ACNGT 2 cut(s) 194, 342
HpyCH4V TGCA 5 cut(s) 129, 146, 188, 269, 479
HpyF10VI GCNNNNNNNGC 3 cut(s) 135, 312, 318
Hsp92I GRCGYC 1 cut(s) 71
Hsp92II CATG 3 cut(s) 157, 181, 508
HspAI GCGC 2 cut(s) 132, 353
Kzo9I GATC 4 cut(s) 81, 100, 112, 415
LmnI GCTCC 3 cut(s) 91, 392, 483
Lsp1109I GCAGC 2 cut(s) 141, 324
MaeI CTAG 1 cut(s) 511
MaeIII GTNAC 1 cut(s) 220
MalI GATC 4 cut(s) 83, 102, 114, 417
MboI GATC 4 cut(s) 81, 100, 112, 415
MboII GAAGA 1 cut(s) 161
MflI RGATCY 3 cut(s) 81, 100, 112
MhlI GDGCHC 1 cut(s) 96
MlyI GAGTC 1 cut(s) 355
MnlI CCTC 3 cut(s) 126, 133, 246
MseI TTAA 2 cut(s) 252, 300
Mva1269I GAATGC 1 cut(s) 269
MwoI GCNNNNNNNGC 3 cut(s) 135, 312, 318
NcoI CCATGG 1 cut(s) 504
NdeI CATATG 1 cut(s) 441
NdeII GATC 4 cut(s) 81, 100, 112, 415
NlaIII CATG 3 cut(s) 157, 181, 508
NlaIV GGNNCC 4 cut(s) 183, 196, 426, 491
NmuCI GTSAC 1 cut(s) 220
NspI RCATGY 1 cut(s) 157
PaqCI CACCTGC 1 cut(s) 194
PciI ACATGT 1 cut(s) 153
PctI GAATGC 1 cut(s) 269
PkrI GCNGC 2 cut(s) 131, 314
PleI GAGTC 1 cut(s) 354
PpsI GAGTC 1 cut(s) 354
PpuMI RGGWCCY 1 cut(s) 182
PscI ACATGT 1 cut(s) 153
PshBI ATTAAT 1 cut(s) 300
Psp124BI GAGCTC 1 cut(s) 96
Psp5II RGGWCCY 1 cut(s) 182
PspN4I GGNNCC 4 cut(s) 183, 196, 426, 491
PspPI GGNCC 4 cut(s) 137, 182, 194, 489
PspPPI RGGWCCY 1 cut(s) 182
PstI CTGCAG 1 cut(s) 190
PsuI RGATCY 3 cut(s) 81, 100, 112
RsaI GTAC 2 cut(s) 446, 500
RsaNI GTAC 2 cut(s) 445, 499
SacI GAGCTC 1 cut(s) 96
SaqAI TTAA 2 cut(s) 252, 300
SatI GCNGC 2 cut(s) 130, 313
Sau3AI GATC 4 cut(s) 81, 100, 112, 415
Sau96I GGNCC 4 cut(s) 137, 182, 194, 489
SchI GAGTC 1 cut(s) 355
SduI GDGCHC 1 cut(s) 96
SfcI CTRYAG 1 cut(s) 186
SinI GGWCC 3 cut(s) 182, 194, 489
SmlI CTYRAG 1 cut(s) 95
SmoI CTYRAG 1 cut(s) 95
SsiI CCGC 1 cut(s) 79
SspMI CTAG 1 cut(s) 511
SstI GAGCTC 1 cut(s) 96
StyI CCWWGG 1 cut(s) 504
TaaI ACNGT 2 cut(s) 194, 342
TaqI TCGA 2 cut(s) 365, 380
TaqII GACCGA 1 cut(s) 467
TatI WGTACW 1 cut(s) 444
Tru1I TTAA 2 cut(s) 252, 300
Tru9I TTAA 2 cut(s) 252, 300
TscAI CASTG 3 cut(s) 167, 347, 481
TseFI GTSAC 1 cut(s) 220
TseI GCWGC 2 cut(s) 129, 312
Tsp45I GTSAC 1 cut(s) 220
TspRI CASTG 3 cut(s) 167, 347, 481
VpaK11BI GGWCC 3 cut(s) 182, 194, 489
VspI ATTAAT 1 cut(s) 300
XceI RCATGY 1 cut(s) 157
XspI CTAG 1 cut(s) 511
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.