Prupe.5G114000_v2.0.a1

ubiquitin-conjugating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0014896
Physical Location & Seq
Forward (+)
11724261 .. 11727895
3635 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G114000.2

Sequence Viewer

Length: 447 bp
ATGGCGTCGAAACGGATCCTGAAGGAGCTGAAGGACCTCCAGAAAGATCCTCCTACTTCATGCAGCGCTGGGCCAGTTGCAGAAGACATGTTCCACTGGCAAGCGACAATAATGGGTCCTGCTGACAGTCCATATGCAGGTGGAGTGTTCCTTGTCACTATACATTTTCCTCCAGACTATCCTTTTAAACCACCCAAGGTTGCATTCAGGACAAAGGTGTTTCACCCTAATATCAACAGCAATGGTAGCATTTGCCTTGACATTTTGAAAGAACAGTGGAGCCCTGCCCTGACCATATCCAAGGTATTGCTTTCAATCTGTTCACTGTTGACAGACCCCAACCCTGATGACCCTTTGGTTCCGGAGATTGCCCATATGTACAAGACTGACAAGAACAAGTACGAGACCACCGCAAGGAGCTGGACTCAGAAGTATGCTATGGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04013 ko04120 ko04141 ko04624 map04013 map04120 map04141 map04624
Pfam Domains
Protein Families
UQ_con

Protein Analysis

149

Amino Acids

16.52

Weight (kDa)

7.71

Isoelectric Point (pI)

47.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 128
Acc36I ACCTGC 1 cut(s) 128
AccIII TCCGGA 1 cut(s) 361
AciI CCGC 1 cut(s) 411
AclWI GGATC 3 cut(s) 10, 23, 41
AcuI CTGAAG 2 cut(s) 41, 50
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 2 cut(s) 380, 401
AfeI AGCGCT 1 cut(s) 67
AfiI CCNNNNNNNGG 2 cut(s) 137, 414
AflIII ACRYGT 1 cut(s) 87
AgsI TTSAA 2 cut(s) 268, 315
AjuI GAANNNNNNNTTGG 2 cut(s) 188, 220
AluBI AGCT 2 cut(s) 28, 420
AluI AGCT 2 cut(s) 28, 420
Alw26I GTCTC 1 cut(s) 398
AlwI GGATC 3 cut(s) 10, 23, 41
Aor13HI TCCGGA 1 cut(s) 361
Aor51HI AGCGCT 1 cut(s) 67
AoxI GGCC 1 cut(s) 71
ApeKI GCWGC 1 cut(s) 63
AspLEI GCGC 1 cut(s) 68
AspS9I GGNCC 3 cut(s) 34, 71, 116
AsuHPI GGTGA 1 cut(s) 215
AvaII GGWCC 2 cut(s) 34, 116
BamHI GGATCC 1 cut(s) 15
BanII GRGCYC 1 cut(s) 284
BbsI GAAGAC 1 cut(s) 90
BbvI GCAGC 1 cut(s) 75
BcoDI GTCTC 1 cut(s) 398
BfoI RGCGCY 1 cut(s) 69
BfuAI ACCTGC 1 cut(s) 128
BisI GCNGC 1 cut(s) 64
BlsI GCNGC 1 cut(s) 65
Bme18I GGWCC 2 cut(s) 34, 116
BmgT120I GGNCC 3 cut(s) 34, 71, 116
BmiI GGNNCC 4 cut(s) 17, 117, 281, 360
BpiI GAAGAC 1 cut(s) 90
BplI GAGNNNNNCTC 2 cut(s) 409, 441
BpmI CTGGAG 2 cut(s) 23, 156
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 398
BsaJI CCNNGG 2 cut(s) 195, 300
BsaWI WCCGGW 1 cut(s) 361
Bsc4I CCNNNNNNNGG 2 cut(s) 137, 414
Bse1I ACTGG 2 cut(s) 74, 101
Bse3DI GCAATG 1 cut(s) 247
BseAI TCCGGA 1 cut(s) 361
BseDI CCNNGG 2 cut(s) 195, 300
BseLI CCNNNNNNNGG 2 cut(s) 137, 414
BseMI GCAATG 1 cut(s) 247
BseMII CTCAG 1 cut(s) 440
BseNI ACTGG 2 cut(s) 74, 101
BseXI GCAGC 1 cut(s) 75
BseYI CCCAGC 1 cut(s) 68
BshFI GGCC 1 cut(s) 73
BsiSI CCGG 1 cut(s) 362
BslI CCNNNNNNNGG 2 cut(s) 137, 414
BsmAI GTCTC 1 cut(s) 398
BsmI GAATGC 1 cut(s) 203
BsnI GGCC 1 cut(s) 73
Bso31I GGTCTC 1 cut(s) 398
Bsp1286I GDGCHC 1 cut(s) 284
Bsp13I TCCGGA 1 cut(s) 361
Bsp1407I TGTACA 1 cut(s) 378
Bsp143I GATC 2 cut(s) 15, 46
BspACI CCGC 1 cut(s) 411
BspANI GGCC 1 cut(s) 73
BspCNI CTCAG 1 cut(s) 439
BspEI TCCGGA 1 cut(s) 361
BspLI GGNNCC 4 cut(s) 17, 117, 281, 360
BspMI ACCTGC 1 cut(s) 128
BspPI GGATC 3 cut(s) 10, 23, 41
BspTNI GGTCTC 1 cut(s) 398
BsrDI GCAATG 1 cut(s) 247
BsrGI TGTACA 1 cut(s) 378
BsrI ACTGG 2 cut(s) 74, 101
BssECI CCNNGG 2 cut(s) 195, 300
BssMI GATC 2 cut(s) 15, 46
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 2 cut(s) 195, 300
Bst4CI ACNGT 3 cut(s) 128, 276, 327
BstACI GRCGYC 1 cut(s) 5
BstAUI TGTACA 1 cut(s) 378
BstC8I GCNNGC 1 cut(s) 102
BstDEI CTNAG 1 cut(s) 426
BstH2I RGCGCY 1 cut(s) 69
BstHHI GCGC 1 cut(s) 68
BstKTI GATC 2 cut(s) 18, 49
BstMAI GTCTC 1 cut(s) 398
BstMBI GATC 2 cut(s) 15, 46
BstMWI GCNNNNNNNGC 1 cut(s) 246
BstNSI RCATGY 1 cut(s) 91
BstV1I GCAGC 1 cut(s) 75
BstV2I GAAGAC 1 cut(s) 90
BstX2I RGATCY 2 cut(s) 15, 46
BstYI RGATCY 2 cut(s) 15, 46
BsuRI GGCC 1 cut(s) 73
BtsIMutI CAGTG 3 cut(s) 94, 281, 323
BveI ACCTGC 1 cut(s) 128
Cac8I GCNNGC 1 cut(s) 102
CfoI GCGC 1 cut(s) 68
Cfr13I GGNCC 3 cut(s) 34, 71, 116
Csp6I GTAC 2 cut(s) 379, 400
CviAII CATG 2 cut(s) 60, 88
CviJI RGCY 4 cut(s) 28, 73, 282, 420
CviKI_1 RGCY 4 cut(s) 28, 73, 282, 420
CviQI GTAC 2 cut(s) 379, 400
DdeI CTNAG 1 cut(s) 426
DpnI GATC 2 cut(s) 17, 48
DpnII GATC 2 cut(s) 15, 46
DraI TTTAAA 1 cut(s) 187
Eco130I CCWWGG 2 cut(s) 195, 300
Eco24I GRGCYC 1 cut(s) 284
Eco31I GGTCTC 1 cut(s) 398
Eco47I GGWCC 2 cut(s) 34, 116
Eco47III AGCGCT 1 cut(s) 67
Eco57I CTGAAG 2 cut(s) 41, 50
EcoO109I RGGNCCY 2 cut(s) 34, 116
EcoT14I CCWWGG 2 cut(s) 195, 300
EcoT38I GRGCYC 1 cut(s) 284
ErhI CCWWGG 2 cut(s) 195, 300
FaeI CATG 2 cut(s) 63, 91
FatI CATG 2 cut(s) 59, 87
FauNDI CATATG 2 cut(s) 133, 375
Fnu4HI GCNGC 1 cut(s) 64
FriOI GRGCYC 1 cut(s) 284
Fsp4HI GCNGC 1 cut(s) 64
GlaI GCGC 1 cut(s) 67
GluI GCNGC 1 cut(s) 64
GsaI CCCAGC 1 cut(s) 72
GsuI CTGGAG 2 cut(s) 23, 156
HaeII RGCGCY 1 cut(s) 69
HaeIII GGCC 1 cut(s) 73
HapII CCGG 1 cut(s) 362
HhaI GCGC 1 cut(s) 68
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 2 cut(s) 63, 91
Hin6I GCGC 1 cut(s) 66
HinP1I GCGC 1 cut(s) 66
HincII GTYRAC 1 cut(s) 330
HindII GTYRAC 1 cut(s) 330
HinfI GANTC 1 cut(s) 424
HpaII CCGG 1 cut(s) 362
HphI GGTGA 1 cut(s) 215
Hpy166II GTNNAC 2 cut(s) 323, 330
Hpy188I TCNGA 1 cut(s) 429
Hpy188III TCNNGA 5 cut(s) 19, 40, 173, 208, 362
Hpy8I GTNNAC 2 cut(s) 323, 330
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 2 cut(s) 16, 25
HpyCH4III ACNGT 3 cut(s) 128, 276, 327
HpyCH4V TGCA 4 cut(s) 63, 80, 137, 203
HpyF10VI GCNNNNNNNGC 1 cut(s) 246
HpyF3I CTNAG 1 cut(s) 426
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 2 cut(s) 63, 91
HspAI GCGC 1 cut(s) 66
Kpn2I TCCGGA 1 cut(s) 361
Kzo9I GATC 2 cut(s) 15, 46
LmnI GCTCC 3 cut(s) 25, 279, 417
Lsp1109I GCAGC 1 cut(s) 75
MaeIII GTNAC 1 cut(s) 154
MalI GATC 2 cut(s) 17, 48
MboI GATC 2 cut(s) 15, 46
MboII GAAGA 1 cut(s) 95
MflI RGATCY 2 cut(s) 15, 46
MhlI GDGCHC 1 cut(s) 284
MlyI GAGTC 1 cut(s) 418
MnlI CCTC 3 cut(s) 47, 60, 180
MroI TCCGGA 1 cut(s) 361
MseI TTAA 1 cut(s) 186
MspI CCGG 1 cut(s) 362
Mva1269I GAATGC 1 cut(s) 203
MwoI GCNNNNNNNGC 1 cut(s) 246
NdeI CATATG 2 cut(s) 133, 375
NdeII GATC 2 cut(s) 15, 46
NlaIII CATG 2 cut(s) 63, 91
NlaIV GGNNCC 4 cut(s) 17, 117, 281, 360
NmuCI GTSAC 1 cut(s) 154
NspI RCATGY 1 cut(s) 91
PaqCI CACCTGC 1 cut(s) 128
PciI ACATGT 1 cut(s) 87
PctI GAATGC 1 cut(s) 203
PkrI GCNGC 1 cut(s) 65
PleI GAGTC 1 cut(s) 418
PpsI GAGTC 1 cut(s) 418
PpuMI RGGWCCY 2 cut(s) 34, 116
PscI ACATGT 1 cut(s) 87
Psp5II RGGWCCY 2 cut(s) 34, 116
PspFI CCCAGC 1 cut(s) 68
PspN4I GGNNCC 4 cut(s) 17, 117, 281, 360
PspPI GGNCC 3 cut(s) 34, 71, 116
PspPPI RGGWCCY 2 cut(s) 34, 116
PsuI RGATCY 2 cut(s) 15, 46
RsaI GTAC 2 cut(s) 380, 401
RsaNI GTAC 2 cut(s) 379, 400
SaqAI TTAA 1 cut(s) 186
SatI GCNGC 1 cut(s) 64
Sau3AI GATC 2 cut(s) 15, 46
Sau96I GGNCC 3 cut(s) 34, 71, 116
SchI GAGTC 1 cut(s) 418
SduI GDGCHC 1 cut(s) 284
SetI ASST 7 cut(s) 30, 39, 142, 201, 219, 306, 422
SinI GGWCC 2 cut(s) 34, 116
SsiI CCGC 1 cut(s) 411
StyI CCWWGG 2 cut(s) 195, 300
TaaI ACNGT 3 cut(s) 128, 276, 327
TaqI TCGA 1 cut(s) 8
TatI WGTACW 1 cut(s) 378
Tru1I TTAA 1 cut(s) 186
Tru9I TTAA 1 cut(s) 186
TscAI CASTG 3 cut(s) 101, 281, 330
TseFI GTSAC 1 cut(s) 154
TseI GCWGC 1 cut(s) 63
Tsp45I GTSAC 1 cut(s) 154
TspDTI ATGAA 1 cut(s) 48
TspGWI ACGGA 1 cut(s) 28
TspRI CASTG 3 cut(s) 101, 281, 330
VpaK11BI GGWCC 2 cut(s) 34, 116
XceI RCATGY 1 cut(s) 91
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.