Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0009840

Physical Location & Seq

Genomic Coordinates Forward (+)

24995204 .. 25000071

Gene Structure 4868 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1111100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2049 bp

ATGGCGAATCGGGAAGGCGGCGATCTGTATCCGGAGCTATGGAAGGCCTGCGCGGGCCCACTCGTCGATGTGCCTCTCGTCCAGGAGAGAGTCCTCTACTTTCCACAGGGGCACATGGAACAATTGGAGGCGTCAACGCCAACAAATCAGGAACTGAATCAAGGGATTCCGCAGTTCAATCTTCCCTCGAAGATTCTTTGCCGCGTCGTTAACGTTAGCCTACTGGCTGAGCAGGAAACAGATGAGGTTTATGCACAGATTACTTTAATTCCAGATGCAAATCAAACGGAACCTACTAGTCCTGATCCATGCCTTGCTGAACCTCAAAGACCTGCAGTTCACTCATTCTGCAAAGTTTTAACTGCCTCTGATACGAGCACCCATGGCGGGTTTTCTGTTCTTCGGAAACACGCCACTGAATGCCTTCCTCCACTGGACATGACCCAGGCAACCCCAACTCAGGAATTGGTTGCCAAGGATCTTCATGGTTACGAGTGGCGATTTAAGCATATTTTCAGAGGTCAACCACGGAGGCACTTGCTTACAACAGGATGGAGTACTTTTGTCACCTCCAAGAGATTAAGTGCTGGAGATTCCTTTGTATTTCTCAGAGGGATCAACGGGGAGTTACGTGTTGGAGTTAGACGTCTTGCTCGTCAGCAGAGCAGCATGCCATCATCTGTGATCTCGAGTCAAAGCATGCATGTAGGGGTGCTCGCAACTGCATCTCATGCACTTGCAACCCAAACTCTTTTTGTCGTCTATTATAAACCAAGGACTAGTCAGTTCATTATTGGTTTGAACAAGTATTTGGAGGCTGTCAACAATAAGTTTTCAGTCGGAATTAGATTCAAAATGAGGTTCGAGGGGGAGGATGCTCCTGAGAGAAGGTTTTCAGGCACAATTGTTGGTCTTGAAGATATATCTCCTCACTGGGCAGATTCAAAATGGCGATCACTTAAAGTTCACTGGGACGAATCTGCATCCATTCCAAGGCCTGACAAGGTATCGCCTTGGGAGATAGAACCTTTTGTGGCGTCTGTACCTCCAAGCCTTCCTCAACCAGCTGTGGTGAAGAACAAAAGGCCTCGACCACCCACTGAAATCCCAGGTCTTGATGCAATGAGTTCAACAGCGTCAGCTATGTGGAATTCTGGGCTGTCACAGTCCCATGACAAGTCACCACCGAGTGTCTCTGCTGAAGGCAAAAGAAGTGAAAATCATGTCGGATGGCAACACCAACAGGCAGATGTGATCAGCAACAACTCTGTTCCAAGGATTCAGACTGATGTGGGATGGTTGTCTTCTCAGGCAGGTGGTTCTCCGCACATGTTTCAGGAAACAATGGAGGATAGTAAAAGTGCTTCTGCTTGGCCTGTTTTCTCTGGATATTCGACTCCAAACTCATCAAAGCTGAAAAATGACTCAATGTTTGACCATGTTGAAAAAGAGAAGAAAACTGAGACAGCTACTAGTTGTCGAATCTTTGGAATAGACTTTACAAGTCATTCTACAAGCTCCCCTGCCATGGAGAAGGTACCTCTGCAACCAATTAGTGGATCTACTGGAACACCTGAAGGACGTGTTAGTAACATGTTAGCAACCGAGTCAGACCAGAAATCTGACCTTTCAAAGACTTCTAAAGAAGGCAAACCTGGACAGCTGCAACTATCACCGAAGGAAACACAGAGCAAGCATAGTTGCTCTATGTCCGCCAGAAGTCGCACCAAGGTTCAAATGCAGGGGATAGCTGTTGGCCGAGCAGTGGACTTGACGACGTTGGAAGGATATGATCAGCTTATAGATGAACTGGAGGAGATGTTTGACATCAAGGGACAGATTCGCCCCGGTAACATTTGGCAAATTGTCTTTACTGATAATGAAGGAGATATGATGCTTATGGGTGATGACCCGTGGTCGGAATTCTGTGACATGGTGAAAAGAATCTTTATATGTTCGAGTCAAGACATGAAGAAAATTAGCGCCGGCTGCAAACTTCCAATGTCGTCACAAGAAGTTGAAGGGGGACCGGTAATAAGCTCGGACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04075 map04075

Pfam Domains

Protein Families

AUX_IAA Auxin_resp B3

Protein Analysis

683

Amino Acids

75.34

Weight (kDa)

6.1

Isoelectric Point (pI)

53.57

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
B3	PF02362	116 - 216	4.1e-22	B3 DNA binding domain
BRWD_AD	PF25313	254 - 344	2.1e-08	BRWD/PHIP ancillary domain-like domain
ARF_AD	PF06507	263 - 344	1.1e-34	Auxin response factor ancillary domain
AUX_IAA	PF02309	555 - 609	2.1e-06	AUX/IAA family
AUX_IAA	PF02309	620 - 657	1e-05	AUX/IAA family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0009840)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G23980 AT4G23980
fragaria_vesca	FvH4_5g11690 FvH4_5g11690 FvH4_5g11690 FvH4_5g11690 FvH4_5g11690
malus_domestica	MD06G1111100.v1.1 MD14G1131900.v1.1
prunus_persica	Prupe.5G123400_v2.0.a1
pyrus_communis	pycom06g10410
rosa_chinensis	RchiOBHm_Chr7g0186081
rosa_laevigata	RLG00000004866
rosa_multiflora	Rmu_sc0000194.1_g000026
rosa_roxburghii	Rroxscaffold_3G00268450
rosa_rugosa	Rorug06G0472100
rosa_samantha	Rh7AG069800 Rh7BG085600 Rh7CG078500 Rh7DG078500
rosa_wichuraiana	Rw7G006530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	768
AarI	CACCTGC	1 cut(s)	1304
AatII	GACGTC	1 cut(s)	649
Acc36I	ACCTGC	2 cut(s)	340, 1304
Acc65I	GGTACC	1 cut(s)	1537
AccB1I	GGYRCC	1 cut(s)	1537
AccB7I	CCANNNNNTGG	1 cut(s)	1556
AccII	CGCG	2 cut(s)	53, 204
AccIII	TCCGGA	1 cut(s)	31
AciI	CCGC	7 cut(s)	18, 53, 170, 202, 387, 1325, 1713
AclI	AACGTT	1 cut(s)	213
AclWI	GGATC	4 cut(s)	299, 486, 623, 1567
AcoI	YGGCCR	1 cut(s)	1756
AcsI	RAATTY	2 cut(s)	1150, 1922
AcuI	CTGAAG	2 cut(s)	1221, 1596
AcyI	GRCGYC	3 cut(s)	131, 646, 1037
AdeI	CACNNNGTG	1 cut(s)	1190
AfaI	GTAC	3 cut(s)	559, 1044, 1539
AfiI	CCNNNNNNNGG	7 cut(s)	387, 460, 993, 1528, 1556, 1765, 1918
AflIII	ACRYGT	4 cut(s)	631, 1329, 1582, 1593
AgeI	ACCGGT	1 cut(s)	2029
AhdI	GACNNNNNGTC	1 cut(s)	1915
AhlI	ACTAGT	3 cut(s)	296, 779, 1472
AjiI	CACGTC	1 cut(s)	1583
AjnI	CCWGG	4 cut(s)	81, 444, 1108, 1654
AjuI	GAANNNNNNNTTGG	2 cut(s)	794, 826
Alw21I	GWGCWC	2 cut(s)	380, 717
Alw26I	GTCTC	2 cut(s)	1198, 1457
AlwI	GGATC	4 cut(s)	299, 486, 623, 1567
AlwNI	CAGNNNCTG	1 cut(s)	154
Ama87I	CYCGRG	1 cut(s)	688
Aor13HI	TCCGGA	1 cut(s)	31
AoxI	GGCC	6 cut(s)	45, 55, 995, 1085, 1373, 1756
ApaI	GGGCCC	1 cut(s)	59
ApeKI	GCWGC	3 cut(s)	666, 1663, 1989
ApoI	RAATTY	2 cut(s)	1150, 1922
ArsI	GACNNNNNNTTYG	2 cut(s)	1415, 1447
AsiGI	ACCGGT	1 cut(s)	2029
Asp700I	GAANNNNTTC	2 cut(s)	423, 892
Asp718I	GGTACC	1 cut(s)	1537
AspLEI	GCGC	2 cut(s)	53, 1985
AspS9I	GGNCC	3 cut(s)	55, 56, 2027
AsuC2I	CCSGG	1 cut(s)	1848
AsuHPI	GGTGA	6 cut(s)	559, 1084, 1173, 1665, 1916, 1948
AvaI	CYCGRG	1 cut(s)	688
AvaII	GGWCC	1 cut(s)	2027
BaeGI	GKGCMC	2 cut(s)	59, 114
BanI	GGYRCC	1 cut(s)	1537
BanII	GRGCYC	1 cut(s)	59
BarI	GAAGNNNNNNTAC	2 cut(s)	1348, 1380
BbsI	GAAGAC	1 cut(s)	1296
Bbv12I	GWGCWC	2 cut(s)	380, 717
BbvI	GCAGC	3 cut(s)	678, 1650, 1976
BccI	CCATC	4 cut(s)	546, 682, 1224, 1290
BciT130I	CCWGG	4 cut(s)	83, 446, 1110, 1656
BciVI	GTATCC	1 cut(s)	39
BclI	TGATCA	2 cut(s)	1254, 1792
BcnI	CCSGG	1 cut(s)	1848
BcoDI	GTCTC	2 cut(s)	1198, 1457
BcuI	ACTAGT	3 cut(s)	296, 779, 1472
BfaI	CTAG	3 cut(s)	297, 780, 1473
BfmI	CTRYAG	1 cut(s)	333
BfoI	RGCGCY	1 cut(s)	1986
BfuAI	ACCTGC	2 cut(s)	340, 1304
BfuI	GTATCC	1 cut(s)	39
BisI	GCNGC	5 cut(s)	19, 202, 667, 1664, 1990
BlpI	GCTNAGC	1 cut(s)	228
BlsI	GCNGC	5 cut(s)	20, 203, 668, 1665, 1991
BmcAI	AGTACT	1 cut(s)	559
Bme1390I	CCNGG	5 cut(s)	83, 446, 1110, 1656, 1848
Bme18I	GGWCC	1 cut(s)	2027
BmeRI	GACNNNNNGTC	1 cut(s)	1915
BmeT110I	CYCGRG	1 cut(s)	688
BmgBI	CACGTC	1 cut(s)	1583
BmgT120I	GGNCC	3 cut(s)	55, 56, 2027
BmiI	GGNNCC	4 cut(s)	57, 291, 1539, 2028
BmrFI	CCNGG	5 cut(s)	83, 446, 1110, 1656, 1848
BmrI	ACTGGG	2 cut(s)	943, 979
BmsI	GCATC	6 cut(s)	265, 734, 865, 992, 1108, 1884
BmuI	ACTGGG	2 cut(s)	943, 979
BpiI	GAAGAC	1 cut(s)	1296
BpmI	CTGGAG	2 cut(s)	609, 1832
Bpu1102I	GCTNAGC	1 cut(s)	228
BpuMI	CCSGG	1 cut(s)	1848
BsaAI	YACGTR	1 cut(s)	632
BsaBI	GATNNNNATC	2 cut(s)	27, 279
BsaHI	GRCGYC	3 cut(s)	131, 646, 1037
BsaWI	WCCGGW	2 cut(s)	31, 2029
BsaXI	ACNNNNNCTCC	2 cut(s)	1805, 1835
Bsc4I	CCNNNNNNNGG	7 cut(s)	387, 460, 993, 1528, 1556, 1765, 1918
Bse118I	RCCGGY	2 cut(s)	1985, 2029
Bse1I	ACTGG	6 cut(s)	228, 438, 938, 974, 1570, 1815
Bse3DI	GCAATG	1 cut(s)	1128
Bse8I	GATNNNNATC	2 cut(s)	27, 279
BseAI	TCCGGA	1 cut(s)	31
BseBI	CCWGG	4 cut(s)	83, 446, 1110, 1656
BseGI	GGATG	5 cut(s)	557, 880, 983, 1235, 1301
BseJI	GATNNNNATC	2 cut(s)	27, 279
BseLI	CCNNNNNNNGG	7 cut(s)	387, 460, 993, 1528, 1556, 1765, 1918
BseMI	GCAATG	1 cut(s)	1128
BseMII	CTCAG	6 cut(s)	219, 473, 622, 873, 1322, 1452
BseNI	ACTGG	6 cut(s)	228, 438, 938, 974, 1570, 1815
BseRI	GAGGAG	2 cut(s)	918, 1829
BseSI	GKGCMC	2 cut(s)	59, 114
BseXI	GCAGC	3 cut(s)	678, 1650, 1976
Bsh1236I	CGCG	2 cut(s)	53, 204
BshFI	GGCC	6 cut(s)	47, 57, 997, 1087, 1375, 1758
BshNI	GGYRCC	1 cut(s)	1537
BshTI	ACCGGT	1 cut(s)	2029
BsiHKAI	GWGCWC	2 cut(s)	380, 717
BsiHKCI	CYCGRG	1 cut(s)	688
BsiSI	CCGG	4 cut(s)	32, 1848, 1986, 2030
BslFI	GGGAC	4 cut(s)	986, 1153, 1848, 2040
BslI	CCNNNNNNNGG	7 cut(s)	387, 460, 993, 1528, 1556, 1765, 1918
BsmAI	GTCTC	2 cut(s)	1198, 1457
BsmFI	GGGAC	4 cut(s)	986, 1153, 1848, 2040
BsmI	GAATGC	1 cut(s)	425
BsnI	GGCC	6 cut(s)	47, 57, 997, 1087, 1375, 1758
BsoBI	CYCGRG	1 cut(s)	688
Bsp120I	GGGCCC	1 cut(s)	55
Bsp1286I	GDGCHC	4 cut(s)	59, 114, 380, 717
Bsp13I	TCCGGA	1 cut(s)	31
Bsp143I	GATC	9 cut(s)	22, 304, 478, 615, 684, 953, 1254, 1559, 1792
Bsp1720I	GCTNAGC	1 cut(s)	228
Bsp19I	CCATGG	2 cut(s)	382, 1527
BspACI	CCGC	7 cut(s)	18, 53, 170, 202, 387, 1325, 1713
BspANI	GGCC	6 cut(s)	47, 57, 997, 1087, 1375, 1758
BspCNI	CTCAG	6 cut(s)	220, 472, 621, 874, 1321, 1453
BspEI	TCCGGA	1 cut(s)	31
BspFNI	CGCG	2 cut(s)	53, 204
BspLI	GGNNCC	4 cut(s)	57, 291, 1539, 2028
BspMAI	CTGCAG	1 cut(s)	337
BspMI	ACCTGC	2 cut(s)	340, 1304
BspPI	GGATC	4 cut(s)	299, 486, 623, 1567
BspT107I	GGYRCC	1 cut(s)	1537
BsrDI	GCAATG	1 cut(s)	1128
BsrFI	RCCGGY	2 cut(s)	1985, 2029
BsrI	ACTGG	6 cut(s)	228, 438, 938, 974, 1570, 1815
BssAI	RCCGGY	2 cut(s)	1985, 2029
BssMI	GATC	9 cut(s)	22, 304, 478, 615, 684, 953, 1254, 1559, 1792
BssNI	GRCGYC	3 cut(s)	131, 646, 1037
BssT1I	CCWWGG	8 cut(s)	382, 474, 773, 992, 1013, 1274, 1527, 1728
Bst2UI	CCWGG	4 cut(s)	83, 446, 1110, 1656
Bst4CI	ACNGT	1 cut(s)	1167
BstACI	GRCGYC	3 cut(s)	131, 646, 1037
BstAPI	GCANNNNNTGC	1 cut(s)	731
BstBAI	YACGTR	1 cut(s)	632
BstC8I	GCNNGC	7 cut(s)	49, 55, 671, 701, 717, 1694, 1987
BstDEI	CTNAG	6 cut(s)	228, 459, 608, 882, 1308, 1461
BstDSI	CCRYGG	4 cut(s)	382, 527, 1527, 1913
BstF5I	GGATG	5 cut(s)	557, 880, 983, 1235, 1301
BstFNI	CGCG	2 cut(s)	53, 204
BstH2I	RGCGCY	1 cut(s)	1986
BstHHI	GCGC	2 cut(s)	53, 1985
BstKTI	GATC	9 cut(s)	25, 307, 481, 618, 687, 956, 1257, 1562, 1795
BstMAI	GTCTC	2 cut(s)	1198, 1457
BstMBI	GATC	9 cut(s)	22, 304, 478, 615, 684, 953, 1254, 1559, 1792
BstMWI	GCNNNNNNNGC	4 cut(s)	384, 505, 731, 1989
BstNI	CCWGG	4 cut(s)	83, 446, 1110, 1656
BstNSI	RCATGY	5 cut(s)	673, 703, 707, 1333, 1597
BstSCI	CCNGG	5 cut(s)	81, 444, 1108, 1654, 1846
BstSFI	CTRYAG	1 cut(s)	333
BstSLI	GKGCMC	2 cut(s)	59, 114
BstUI	CGCG	2 cut(s)	53, 204
BstV1I	GCAGC	3 cut(s)	678, 1650, 1976
BstV2I	GAAGAC	1 cut(s)	1296
BstX2I	RGATCY	2 cut(s)	478, 1559
BstYI	RGATCY	2 cut(s)	478, 1559
BsuI	GTATCC	1 cut(s)	39
BsuRI	GGCC	6 cut(s)	47, 57, 997, 1087, 1375, 1758
BtgI	CCRYGG	4 cut(s)	382, 527, 1527, 1913
BtrI	CACGTC	1 cut(s)	1583
BtsCI	GGATG	5 cut(s)	557, 880, 983, 1235, 1301
BtsI	GCAGTG	1 cut(s)	1770
BtsIMutI	CAGTG	6 cut(s)	414, 431, 931, 967, 1098, 1770
BveI	ACCTGC	2 cut(s)	340, 1304
Cac8I	GCNNGC	7 cut(s)	49, 55, 671, 701, 717, 1694, 1987
CaiI	CAGNNNCTG	1 cut(s)	154
CfoI	GCGC	2 cut(s)	53, 1985
Cfr10I	RCCGGY	2 cut(s)	1985, 2029
Cfr13I	GGNCC	3 cut(s)	55, 56, 2027
CseI	GACGC	4 cut(s)	120, 193, 1026, 1125
Csp6I	GTAC	3 cut(s)	558, 1043, 1538
CspAI	ACCGGT	1 cut(s)	2029
CviQI	GTAC	3 cut(s)	558, 1043, 1538
DdeI	CTNAG	6 cut(s)	228, 459, 608, 882, 1308, 1461
DpnI	GATC	9 cut(s)	24, 306, 480, 617, 686, 955, 1256, 1561, 1794
DpnII	GATC	9 cut(s)	22, 304, 478, 615, 684, 953, 1254, 1559, 1792
DraIII	CACNNNGTG	1 cut(s)	1190
DriI	GACNNNNNGTC	1 cut(s)	1915
EaeI	YGGCCR	1 cut(s)	1756
Eam1105I	GACNNNNNGTC	1 cut(s)	1915
EciI	GGCGGA	1 cut(s)	1702
Eco130I	CCWWGG	8 cut(s)	382, 474, 773, 992, 1013, 1274, 1527, 1728
Eco147I	AGGCCT	3 cut(s)	47, 997, 1087
Eco24I	GRGCYC	1 cut(s)	59
Eco47I	GGWCC	1 cut(s)	2027
Eco57I	CTGAAG	2 cut(s)	1221, 1596
Eco88I	CYCGRG	1 cut(s)	688
EcoRI	GAATTC	2 cut(s)	1150, 1922
EcoRII	CCWGG	4 cut(s)	81, 444, 1108, 1654
EcoT14I	CCWWGG	8 cut(s)	382, 474, 773, 992, 1013, 1274, 1527, 1728
EcoT22I	ATGCAT	1 cut(s)	705
EcoT38I	GRGCYC	1 cut(s)	59
ErhI	CCWWGG	8 cut(s)	382, 474, 773, 992, 1013, 1274, 1527, 1728
FaqI	GGGAC	4 cut(s)	986, 1153, 1848, 2040
FauI	CCCGC	2 cut(s)	46, 380
FbaI	TGATCA	2 cut(s)	1254, 1792
Fnu4HI	GCNGC	5 cut(s)	19, 202, 667, 1664, 1990
FokI	GGATG	5 cut(s)	564, 887, 970, 1242, 1308
FriOI	GRGCYC	1 cut(s)	59
Fsp4HI	GCNGC	5 cut(s)	19, 202, 667, 1664, 1990
FspBI	CTAG	3 cut(s)	297, 780, 1473
GlaI	GCGC	2 cut(s)	52, 1984
GluI	GCNGC	5 cut(s)	19, 202, 667, 1664, 1990
GsuI	CTGGAG	2 cut(s)	609, 1832
HaeII	RGCGCY	1 cut(s)	1986
HaeIII	GGCC	6 cut(s)	47, 57, 997, 1087, 1375, 1758
HapII	CCGG	4 cut(s)	32, 1848, 1986, 2030
HgaI	GACGC	4 cut(s)	120, 193, 1026, 1125
HhaI	GCGC	2 cut(s)	53, 1985
Hin1I	GRCGYC	3 cut(s)	131, 646, 1037
Hin6I	GCGC	2 cut(s)	51, 1983
HinP1I	GCGC	2 cut(s)	51, 1983
HincII	GTYRAC	4 cut(s)	135, 211, 524, 823
HindII	GTYRAC	4 cut(s)	135, 211, 524, 823
HpaI	GTTAAC	1 cut(s)	211
HpaII	CCGG	4 cut(s)	32, 1848, 1986, 2030
HphI	GGTGA	6 cut(s)	559, 1084, 1173, 1665, 1916, 1948
Hpy166II	GTNNAC	7 cut(s)	135, 211, 340, 524, 823, 967, 1768
Hpy8I	GTNNAC	7 cut(s)	135, 211, 340, 524, 823, 967, 1768
Hpy99I	CGWCG	3 cut(s)	68, 209, 1780
HpyCH4III	ACNGT	1 cut(s)	1167
HpyCH4IV	ACGT	5 cut(s)	213, 631, 646, 1582, 1778
HpyF10VI	GCNNNNNNNGC	4 cut(s)	384, 505, 731, 1989
HpyF3I	CTNAG	6 cut(s)	228, 459, 608, 882, 1308, 1461
HpySE526I	ACGT	5 cut(s)	213, 631, 646, 1582, 1778
Hsp92I	GRCGYC	3 cut(s)	131, 646, 1037
HspAI	GCGC	2 cut(s)	51, 1983
Kpn2I	TCCGGA	1 cut(s)	31
KpnI	GGTACC	1 cut(s)	1541
KroI	GCCGGC	1 cut(s)	1985
KroNI	GCCGGC	1 cut(s)	1987
Ksp22I	TGATCA	2 cut(s)	1254, 1792
KspAI	GTTAAC	1 cut(s)	211
Kzo9I	GATC	9 cut(s)	22, 304, 478, 615, 684, 953, 1254, 1559, 1792
LmnI	GCTCC	3 cut(s)	34, 883, 1523
Lsp1109I	GCAGC	3 cut(s)	678, 1650, 1976
LweI	GCATC	6 cut(s)	265, 734, 865, 992, 1108, 1884
MaeI	CTAG	3 cut(s)	297, 780, 1473
MaeII	ACGT	5 cut(s)	213, 631, 646, 1582, 1778
MaeIII	GTNAC	9 cut(s)	488, 565, 627, 1161, 1179, 1589, 1850, 1928, 2007
MalI	GATC	9 cut(s)	24, 306, 480, 617, 686, 955, 1256, 1561, 1794
MboI	GATC	9 cut(s)	22, 304, 478, 615, 684, 953, 1254, 1559, 1792
MboII	GAAGA	9 cut(s)	173, 202, 392, 473, 929, 1087, 1296, 1465, 1984
MfeI	CAATTG	2 cut(s)	122, 903
MflI	RGATCY	2 cut(s)	478, 1559
MhlI	GDGCHC	4 cut(s)	59, 114, 380, 717
MlyI	GAGTC	6 cut(s)	99, 700, 1390, 1418, 1616, 1969
MmeI	TCCRAC	5 cut(s)	616, 820, 1207, 1761, 1899
Mph1103I	ATGCAT	1 cut(s)	705
MroI	TCCGGA	1 cut(s)	31
MroNI	GCCGGC	1 cut(s)	1985
MroXI	GAANNNNTTC	2 cut(s)	423, 892
MseI	TTAA	6 cut(s)	210, 266, 359, 504, 581, 960
MspA1I	CMGCKG	2 cut(s)	1067, 1663
MspI	CCGG	4 cut(s)	32, 1848, 1986, 2030
MspR9I	CCNGG	5 cut(s)	83, 446, 1110, 1656, 1848
MunI	CAATTG	2 cut(s)	122, 903
Mva1269I	GAATGC	1 cut(s)	425
MvaI	CCWGG	4 cut(s)	83, 446, 1110, 1656
MvnI	CGCG	2 cut(s)	53, 204
MwoI	GCNNNNNNNGC	4 cut(s)	384, 505, 731, 1989
NaeI	GCCGGC	1 cut(s)	1987
NciI	CCSGG	1 cut(s)	1848
NcoI	CCATGG	2 cut(s)	382, 1527
NdeII	GATC	9 cut(s)	22, 304, 478, 615, 684, 953, 1254, 1559, 1792
NgoMIV	GCCGGC	1 cut(s)	1985
NlaIV	GGNNCC	4 cut(s)	57, 291, 1539, 2028
NmeAIII	GCCGAG	1 cut(s)	1784
NmuCI	GTSAC	5 cut(s)	565, 1161, 1179, 1928, 2007
NsiI	ATGCAT	1 cut(s)	705
NspI	RCATGY	5 cut(s)	673, 703, 707, 1333, 1597
PaeI	GCATGC	2 cut(s)	673, 703
PaeR7I	CTCGAG	1 cut(s)	688
PaqCI	CACCTGC	1 cut(s)	1304
PceI	AGGCCT	3 cut(s)	47, 997, 1087
PciI	ACATGT	2 cut(s)	1329, 1593
PcsI	WCGNNNNNNNCGW	1 cut(s)	652
PctI	GAATGC	1 cut(s)	425
PdiI	GCCGGC	1 cut(s)	1987
PdmI	GAANNNNTTC	2 cut(s)	423, 892
PflMI	CCANNNNNTGG	1 cut(s)	1556
PfoI	TCCNGGA	1 cut(s)	81
PinAI	ACCGGT	1 cut(s)	2029
PkrI	GCNGC	5 cut(s)	20, 203, 668, 1665, 1991
PleI	GAGTC	6 cut(s)	98, 699, 1390, 1418, 1615, 1968
PpsI	GAGTC	6 cut(s)	98, 699, 1390, 1418, 1615, 1968
Ppu21I	YACGTR	1 cut(s)	632
PscI	ACATGT	2 cut(s)	1329, 1593
PsiI	TTATAA	1 cut(s)	768
Psp1406I	AACGTT	1 cut(s)	213
Psp6I	CCWGG	4 cut(s)	81, 444, 1108, 1654
PspGI	CCWGG	4 cut(s)	81, 444, 1108, 1654
PspN4I	GGNNCC	4 cut(s)	57, 291, 1539, 2028
PspOMI	GGGCCC	1 cut(s)	55
PspPI	GGNCC	3 cut(s)	55, 56, 2027
PstI	CTGCAG	1 cut(s)	337
PstNI	CAGNNNCTG	1 cut(s)	154
PsuI	RGATCY	2 cut(s)	478, 1559
PvuII	CAGCTG	2 cut(s)	1067, 1663
RsaI	GTAC	3 cut(s)	559, 1044, 1539
RsaNI	GTAC	3 cut(s)	558, 1043, 1538
SaqAI	TTAA	6 cut(s)	210, 266, 359, 504, 581, 960
SatI	GCNGC	5 cut(s)	19, 202, 667, 1664, 1990
Sau3AI	GATC	9 cut(s)	22, 304, 478, 615, 684, 953, 1254, 1559, 1792
Sau96I	GGNCC	3 cut(s)	55, 56, 2027
ScaI	AGTACT	1 cut(s)	559
SchI	GAGTC	6 cut(s)	99, 700, 1390, 1418, 1616, 1969
ScrFI	CCNGG	5 cut(s)	83, 446, 1110, 1656, 1848
SduI	GDGCHC	4 cut(s)	59, 114, 380, 717
SfaNI	GCATC	6 cut(s)	265, 734, 865, 992, 1108, 1884
SfcI	CTRYAG	1 cut(s)	333
Sfr274I	CTCGAG	1 cut(s)	688
SinI	GGWCC	1 cut(s)	2027
SlaI	CTCGAG	1 cut(s)	688
SmlI	CTYRAG	1 cut(s)	688
SmoI	CTYRAG	1 cut(s)	688
SpeI	ACTAGT	3 cut(s)	296, 779, 1472
SphI	GCATGC	2 cut(s)	673, 703
SseBI	AGGCCT	3 cut(s)	47, 997, 1087
SsiI	CCGC	7 cut(s)	18, 53, 170, 202, 387, 1325, 1713
SspMI	CTAG	3 cut(s)	297, 780, 1473
StuI	AGGCCT	3 cut(s)	47, 997, 1087
StyD4I	CCNGG	5 cut(s)	81, 444, 1108, 1654, 1846
StyI	CCWWGG	8 cut(s)	382, 474, 773, 992, 1013, 1274, 1527, 1728
TaaI	ACNGT	1 cut(s)	1167
TaiI	ACGT	5 cut(s)	216, 634, 649, 1585, 1781
TaqI	TCGA	8 cut(s)	66, 188, 689, 864, 1090, 1394, 1480, 1958
TatI	WGTACW	1 cut(s)	557
TauI	GCSGC	2 cut(s)	21, 204
Tru1I	TTAA	6 cut(s)	210, 266, 359, 504, 581, 960
Tru9I	TTAA	6 cut(s)	210, 266, 359, 504, 581, 960
TscAI	CASTG	6 cut(s)	421, 438, 938, 974, 1105, 1770
TseFI	GTSAC	5 cut(s)	565, 1161, 1179, 1928, 2007
TseI	GCWGC	3 cut(s)	666, 1663, 1989
Tsp45I	GTSAC	5 cut(s)	565, 1161, 1179, 1928, 2007
TspDTI	ATGAA	5 cut(s)	473, 778, 1821, 1896, 1985
TspGWI	ACGGA	2 cut(s)	302, 544
TspRI	CASTG	6 cut(s)	421, 438, 938, 974, 1105, 1770
Van91I	CCANNNNNTGG	1 cut(s)	1556
VpaK11BI	GGWCC	1 cut(s)	2027
XapI	RAATTY	2 cut(s)	1150, 1922
XceI	RCATGY	5 cut(s)	673, 703, 707, 1333, 1597
XhoI	CTCGAG	1 cut(s)	688
XmnI	GAANNNNTTC	2 cut(s)	423, 892
XspI	CTAG	3 cut(s)	297, 780, 1473
ZraI	GACGTC	1 cut(s)	647
ZrmI	AGTACT	1 cut(s)	559
Zsp2I	ATGCAT	1 cut(s)	705

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.