MD06G1133200.v1.1

Transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0011298
Physical Location & Seq
Forward (+)
27729640 .. 27732821
3182 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1133200.v1.1.491

Sequence Viewer

Length: 1089 bp
ATGGCAGCCTTTTCATACCAAAATCTCCACCACCTTTCTCTCCTTGACTCAATTTTCTTGCCCAACGCTCCTACTACCAATAGCATCAACACTGACAGTAACTTCTCTCAATCTTTTTACCCATCTGAGCCTTTAACCCAGGAAATTCAAGCTGATAATAATTCAAGAGCTGTTGAAAGCAGCTGCACTATGGATCATAGCTCTACTAAGGTTGCTTTTAGTGACAATGAGAATGATCCTTCTGTGACCAAAAACAAGAGCACAGAGTCCTCAACTGTTGTGGACGTGGATAGGATTGAAATTGGTGATCAGGTCACTCAGAAAGTGACTCCTATGGGCAAGAAGAGAAAATCCAGCACTGGATCATCCTTCAATTCTGCTCAATCCAAGGGTACTAAAGAGCAGAAGGGAAAGAAGCAAAAGAAGATTGATGGCGGTACCAAGAAAGATGAGGAAAAGAAGATTAAATCAGATGAAGAAGACCAGGTGAAAGTAAGAGAAGGACCTCCAACTGGCTACATTCATGTTAGAGCAAGGAGGGGTCAGGCTACAGATAGTCACAGCCTTGCAGAAAGGGTAAGAAGACAGAAAATTAGTGAGAGGATGAAGATGTTGCAAAGACTTGTTCCTGGCTGTGACAAGATAACTGGAAGGGCAGTCATGCTGGATGAAATTATCAATTATGTTCAGTCCCTACAAAATCAAGTGGAGTTCCTCTCGGTGAAACTTGACTCTTTGAATCCAATGTTCTACGACTTTGGAATCGACATTGGTGCTTTGGTGGTCAAACCAGAGAGATTGTGTAGCACGGAATCACCATATCAATCTGTGCCACAATGCAACCCAACACAGCCAACACCTTTTGCTGATACATCCACCAACATCACCGCCACCACTGCCGTTGCCGCTGCCGCTGCCACCACAAATAACAACTGTCCTTTTCTAGACAGTTCAGCTTCACTTCTACTTCAAAGGCCAAATACCTTCTTTCAGGACGGTGAGAGCTTGTTGTGGGATGTGGAAGATCAAAGACAAAGCTTTCTTAATCCATCTGGTTTCAGCAACAGCAACTTGTGTTCTTTCAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH

Protein Analysis

363

Amino Acids

40.0

Weight (kDa)

6.03

Isoelectric Point (pI)

49.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 186 - 233 1.9e-06 Helix-loop-helix DNA-binding domain
bHLH_SAC51 PF23173 191 - 236 6.3e-07 Transcription factor SAC51 bHLH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011298)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50915 AT5G50915 AT5G50915 AT5G50915
fragaria_vesca FvH4_5g09140 FvH4_5g09140
malus_domestica MD06G1133200.v1.1 MD14G1149600.v1.1
prunus_persica Prupe.5G144400_v2.0.a1
pyrus_communis pycom06g12480 pycom14g12420
rosa_chinensis RchiOBHm_Chr7g0189021
rosa_laevigata RLG00000004638
rosa_roxburghii Rroxscaffold_3G00265840
rosa_rugosa Rorug06G0495700
rosa_samantha Rh7AG100200 Rh7BG102700 Rh7CG104100 Rh7DG102200
rosa_wichuraiana Rw7G008660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 437
AccB1I GGYRCC 1 cut(s) 437
AciI CCGC 4 cut(s) 435, 888, 906, 912
AclWI GGATC 3 cut(s) 201, 230, 370
AcsI RAATTY 1 cut(s) 144
AfaI GTAC 2 cut(s) 394, 439
AfiI CCNNNNNNNGG 1 cut(s) 512
AgsI TTSAA 8 cut(s) 149, 165, 176, 299, 373, 739, 971, 1084
AjiI CACGTC 1 cut(s) 286
AjnI CCWGG 3 cut(s) 138, 483, 628
AluBI AGCT 7 cut(s) 152, 170, 183, 201, 956, 1005, 1038
AluI AGCT 7 cut(s) 152, 170, 183, 201, 956, 1005, 1038
Alw21I GWGCWC 1 cut(s) 263
AlwI GGATC 3 cut(s) 201, 230, 370
AoxI GGCC 1 cut(s) 974
ApeKI GCWGC 5 cut(s) 5, 180, 183, 908, 914
ApoI RAATTY 1 cut(s) 144
Asp718I GGTACC 1 cut(s) 437
AspS9I GGNCC 1 cut(s) 503
AsuHPI GGTGA 6 cut(s) 317, 499, 733, 807, 877, 1010
AvaII GGWCC 1 cut(s) 503
BanI GGYRCC 1 cut(s) 437
BbsI GAAGAC 2 cut(s) 486, 589
Bbv12I GWGCWC 1 cut(s) 263
BbvI GCAGC 5 cut(s) 17, 170, 192, 895, 901
BccI CCATC 3 cut(s) 130, 425, 1057
BceAI ACGGC 1 cut(s) 884
BcgI CGANNNNNNTGC 2 cut(s) 755, 789
BciT130I CCWGG 3 cut(s) 140, 485, 630
BclI TGATCA 1 cut(s) 307
BfaI CTAG 1 cut(s) 944
BfmI CTRYAG 1 cut(s) 549
BisI GCNGC 7 cut(s) 6, 181, 184, 906, 909, 912, 915
BlsI GCNGC 7 cut(s) 7, 182, 185, 907, 910, 913, 916
Bme1390I CCNGG 3 cut(s) 140, 485, 630
Bme18I GGWCC 1 cut(s) 503
BmgBI CACGTC 1 cut(s) 286
BmgT120I GGNCC 1 cut(s) 503
BmiI GGNNCC 1 cut(s) 439
BmrFI CCNGG 3 cut(s) 140, 485, 630
BmsI GCATC 1 cut(s) 93
BpiI GAAGAC 2 cut(s) 486, 589
BplI GAGNNNNNCTC 2 cut(s) 701, 733
BsaJI CCNNGG 2 cut(s) 138, 387
Bsc4I CCNNNNNNNGG 1 cut(s) 512
Bse1I ACTGG 3 cut(s) 364, 517, 652
BseBI CCWGG 3 cut(s) 140, 485, 630
BseDI CCNNGG 2 cut(s) 138, 387
BseGI GGATG 5 cut(s) 365, 609, 673, 872, 1021
BseLI CCNNNNNNNGG 1 cut(s) 512
BseMII CTCAG 2 cut(s) 117, 332
BseNI ACTGG 3 cut(s) 364, 517, 652
BseXI GCAGC 5 cut(s) 17, 170, 192, 895, 901
BsgI GTGCAG 1 cut(s) 169
BshFI GGCC 1 cut(s) 976
BshNI GGYRCC 1 cut(s) 437
BsiHKAI GWGCWC 1 cut(s) 263
BslFI GGGAC 1 cut(s) 676
BslI CCNNNNNNNGG 1 cut(s) 512
BsmFI GGGAC 1 cut(s) 676
BsnI GGCC 1 cut(s) 976
Bsp1286I GDGCHC 1 cut(s) 263
Bsp143I GATC 5 cut(s) 193, 235, 307, 362, 1024
BspACI CCGC 4 cut(s) 435, 888, 906, 912
BspANI GGCC 1 cut(s) 976
BspCNI CTCAG 2 cut(s) 118, 331
BspLI GGNNCC 1 cut(s) 439
BspPI GGATC 3 cut(s) 201, 230, 370
BspT107I GGYRCC 1 cut(s) 437
BsrI ACTGG 3 cut(s) 364, 517, 652
BssECI CCNNGG 2 cut(s) 138, 387
BssMI GATC 5 cut(s) 193, 235, 307, 362, 1024
BssT1I CCWWGG 1 cut(s) 387
Bst2UI CCWGG 3 cut(s) 140, 485, 630
Bst4CI ACNGT 5 cut(s) 98, 277, 935, 950, 998
Bst6I CTCTTC 1 cut(s) 338
BstDEI CTNAG 3 cut(s) 126, 207, 318
BstF5I GGATG 5 cut(s) 365, 609, 673, 872, 1021
BstKTI GATC 5 cut(s) 196, 238, 310, 365, 1027
BstMBI GATC 5 cut(s) 193, 235, 307, 362, 1024
BstMWI GCNNNNNNNGC 4 cut(s) 896, 905, 911, 914
BstNI CCWGG 3 cut(s) 140, 485, 630
BstSCI CCNGG 3 cut(s) 138, 483, 628
BstSFI CTRYAG 1 cut(s) 549
BstV1I GCAGC 5 cut(s) 17, 170, 192, 895, 901
BstV2I GAAGAC 2 cut(s) 486, 589
BsuRI GGCC 1 cut(s) 976
BtrI CACGTC 1 cut(s) 286
BtsCI GGATG 5 cut(s) 365, 609, 673, 872, 1021
BtsI GCAGTG 1 cut(s) 894
BtsIMutI CAGTG 3 cut(s) 90, 357, 894
Cfr13I GGNCC 1 cut(s) 503
CsiI ACCWGGT 1 cut(s) 483
Csp6I GTAC 2 cut(s) 393, 438
CspCI CAANNNNNGTGG 4 cut(s) 261, 296, 883, 918
CviAII CATG 2 cut(s) 524, 661
CviQI GTAC 2 cut(s) 393, 438
DdeI CTNAG 3 cut(s) 126, 207, 318
DpnI GATC 5 cut(s) 195, 237, 309, 364, 1026
DpnII GATC 5 cut(s) 193, 235, 307, 362, 1024
Eam1104I CTCTTC 1 cut(s) 338
EarI CTCTTC 1 cut(s) 338
Eco130I CCWWGG 1 cut(s) 387
Eco47I GGWCC 1 cut(s) 503
EcoO109I RGGNCCY 1 cut(s) 503
EcoRII CCWGG 3 cut(s) 138, 483, 628
EcoT14I CCWWGG 1 cut(s) 387
ErhI CCWWGG 1 cut(s) 387
FaeI CATG 2 cut(s) 527, 664
FaiI YATR 8 cut(s) 16, 191, 198, 335, 525, 662, 684, 820
FaqI GGGAC 1 cut(s) 676
FatI CATG 2 cut(s) 523, 660
FbaI TGATCA 1 cut(s) 307
Fnu4HI GCNGC 7 cut(s) 6, 181, 184, 906, 909, 912, 915
FokI GGATG 5 cut(s) 352, 616, 680, 859, 1028
Fsp4HI GCNGC 7 cut(s) 6, 181, 184, 906, 909, 912, 915
FspBI CTAG 1 cut(s) 944
GluI GCNGC 7 cut(s) 6, 181, 184, 906, 909, 912, 915
HaeIII GGCC 1 cut(s) 976
Hin1II CATG 2 cut(s) 527, 664
HindIII AAGCTT 1 cut(s) 1036
HinfI GANTC 7 cut(s) 47, 266, 328, 731, 739, 762, 812
HphI GGTGA 6 cut(s) 317, 499, 733, 807, 877, 1010
Hpy166II GTNNAC 1 cut(s) 283
Hpy188I TCNGA 3 cut(s) 127, 321, 472
Hpy188III TCNNGA 3 cut(s) 165, 944, 992
Hpy8I GTNNAC 1 cut(s) 283
HpyAV CCTTC 6 cut(s) 249, 379, 400, 494, 645, 994
HpyCH4III ACNGT 5 cut(s) 98, 277, 935, 950, 998
HpyCH4IV ACGT 1 cut(s) 285
HpyCH4V TGCA 4 cut(s) 186, 569, 616, 840
HpyF10VI GCNNNNNNNGC 4 cut(s) 896, 905, 911, 914
HpyF3I CTNAG 3 cut(s) 126, 207, 318
HpySE526I ACGT 1 cut(s) 285
Hsp92II CATG 2 cut(s) 527, 664
KpnI GGTACC 1 cut(s) 441
Ksp22I TGATCA 1 cut(s) 307
Kzo9I GATC 5 cut(s) 193, 235, 307, 362, 1024
LmnI GCTCC 1 cut(s) 73
Lsp1109I GCAGC 5 cut(s) 17, 170, 192, 895, 901
LweI GCATC 1 cut(s) 93
MabI ACCWGGT 1 cut(s) 483
MaeI CTAG 1 cut(s) 944
MaeII ACGT 1 cut(s) 285
MaeIII GTNAC 7 cut(s) 98, 221, 244, 313, 325, 557, 635
MalI GATC 5 cut(s) 195, 237, 309, 364, 1026
MboI GATC 5 cut(s) 193, 235, 307, 362, 1024
MboII GAAGA 8 cut(s) 355, 436, 472, 488, 491, 594, 619, 1034
MhlI GDGCHC 1 cut(s) 263
MluCI AATT 9 cut(s) 51, 144, 160, 300, 373, 591, 672, 679, 1084
MlyI GAGTC 4 cut(s) 41, 275, 322, 725
MmeI TCCRAC 1 cut(s) 533
MnlI CCTC 6 cut(s) 280, 445, 516, 531, 594, 725
MseI TTAA 4 cut(s) 134, 465, 1044, 1087
MspA1I CMGCKG 3 cut(s) 183, 908, 914
MspR9I CCNGG 3 cut(s) 140, 485, 630
MvaI CCWGG 3 cut(s) 140, 485, 630
MwoI GCNNNNNNNGC 4 cut(s) 896, 905, 911, 914
NdeII GATC 5 cut(s) 193, 235, 307, 362, 1024
NlaIII CATG 2 cut(s) 527, 664
NlaIV GGNNCC 1 cut(s) 439
NmuCI GTSAC 6 cut(s) 221, 244, 313, 325, 557, 635
PfeI GAWTC 3 cut(s) 739, 762, 812
PkrI GCNGC 7 cut(s) 7, 182, 185, 907, 910, 913, 916
PleI GAGTC 4 cut(s) 41, 274, 322, 725
PpsI GAGTC 4 cut(s) 41, 274, 322, 725
PpuMI RGGWCCY 1 cut(s) 503
Psp5II RGGWCCY 1 cut(s) 503
Psp6I CCWGG 3 cut(s) 138, 483, 628
PspGI CCWGG 3 cut(s) 138, 483, 628
PspN4I GGNNCC 1 cut(s) 439
PspPI GGNCC 1 cut(s) 503
PspPPI RGGWCCY 1 cut(s) 503
PvuII CAGCTG 1 cut(s) 183
RsaI GTAC 2 cut(s) 394, 439
RsaNI GTAC 2 cut(s) 393, 438
SaqAI TTAA 4 cut(s) 134, 465, 1044, 1087
SatI GCNGC 7 cut(s) 6, 181, 184, 906, 909, 912, 915
Sau3AI GATC 5 cut(s) 193, 235, 307, 362, 1024
Sau96I GGNCC 1 cut(s) 503
SchI GAGTC 4 cut(s) 41, 275, 322, 725
ScrFI CCNGG 3 cut(s) 140, 485, 630
SduI GDGCHC 1 cut(s) 263
SexAI ACCWGGT 1 cut(s) 483
SfaNI GCATC 1 cut(s) 93
SfcI CTRYAG 1 cut(s) 549
SinI GGWCC 1 cut(s) 503
Sse9I AATT 9 cut(s) 51, 144, 160, 300, 373, 591, 672, 679, 1084
SsiI CCGC 4 cut(s) 435, 888, 906, 912
SspMI CTAG 1 cut(s) 944
StyD4I CCNGG 3 cut(s) 138, 483, 628
StyI CCWWGG 1 cut(s) 387
TaaI ACNGT 5 cut(s) 98, 277, 935, 950, 998
TaiI ACGT 1 cut(s) 288
TaqI TCGA 1 cut(s) 765
TasI AATT 9 cut(s) 51, 144, 160, 300, 373, 591, 672, 679, 1084
TauI GCSGC 2 cut(s) 908, 914
TfiI GAWTC 3 cut(s) 739, 762, 812
Tru1I TTAA 4 cut(s) 134, 465, 1044, 1087
Tru9I TTAA 4 cut(s) 134, 465, 1044, 1087
TscAI CASTG 3 cut(s) 97, 364, 901
TseFI GTSAC 6 cut(s) 221, 244, 313, 325, 557, 635
TseI GCWGC 5 cut(s) 5, 180, 183, 908, 914
Tsp45I GTSAC 6 cut(s) 221, 244, 313, 325, 557, 635
TspDTI ATGAA 4 cut(s) 489, 512, 620, 684
TspGWI ACGGA 1 cut(s) 824
TspRI CASTG 3 cut(s) 97, 364, 901
VpaK11BI GGWCC 1 cut(s) 503
XapI RAATTY 1 cut(s) 144
XbaI TCTAGA 1 cut(s) 943
XspI CTAG 1 cut(s) 944
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.