MD14G1149600.v1.1

Transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0011298
Physical Location & Seq
Forward (+)
24247022 .. 24249526
2505 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1149600.v1.1.491

Sequence Viewer

Length: 1077 bp
ATGGCAGCCTTTTCATACAATGGCAGCCTTGACTCAATTTTCTTGCCCAACGCTCCGGCTACCAATGACATCATCATTTACAGTAACTTCTCTCGATCTTTTTACCCATCTGAGCCTTTAACCCGGGAAATTCAAGCTGATAATAATTCAAGAGCTGTTGAAAGCAGCTGCACTATGGATCACAGCTCCACTCATAAGGTTGCTTTTAGTGACAATGAGAATGAGCCTTCTGTGACCAAAAACAAGAGCAGAGAGTCCTCAACTGTTGTGGATGTGGATAGGCTTGAAATTGGTGAGCAGGTCACTCAGAAAGTAACTCCTATGGACAAGAAGAGAAAATCCAGAATTGGGTCATCCTTCAATTCTGCTCAATCCAAGGGTACTAAAGAGCATAAGGGAAAGAAGCAAAAGAAGGCTGGTGGCGGTACCAAGAAGGCTGAGGAAAAGAAGATTAAATCAGATAAAAAAGACCAGGTGAAAGTGAGAGAAGAACCTCCAACTGGCTACATTCATGTTAGAGCAAGGAGGGGTGAGGCTACAGATAGTCACAGCCTTGCAGAAAGGGTGAGAAGAGAGAAAATTAGTGAGAGGATGAAGATGCTGCAAAAACTTGTCCCTGGCTGTGACAAGGTAACTGGAAGGGCAGTCATGCTGGATGAAATTATCAATTATGTTCAGTCCCTACAAAATCAAGTGGAGTTCCTCTCACTGAAACTTGCTTCTTTGAATCCAATGTTCTTTGACTTTGGAATGGACATGGGTGCTTTAGTGGTTAAACCAGAGAGATTGTGTAGCATGGAATCACCATATCCATCTGTGCAACAATGCAACCCAATACAGTCCACACCTTTTGCTGATACATCCACCAATATCACTGCCACCACTGCCGCTGCCACTACCGCCTTCACCACAAATAACAACTATCATTTTCTGGACAATTCAGCTTCACATCTACTTCAAAGGCCAAATACCTTCTCGCAGGACAGTGAGAGCTTGTTGTGGGATGTGGAAGATCAAAGACAAAACTTTCATAATCCATGTGGTTTCAGCAACAGCAACTTGTGTTCTTTGAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH

Protein Analysis

359

Amino Acids

39.97

Weight (kDa)

8.6

Isoelectric Point (pI)

44.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 182 - 229 3.4e-06 Helix-loop-helix DNA-binding domain
bHLH_SAC51 PF23173 187 - 231 1.3e-06 Transcription factor SAC51 bHLH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011298)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50915 AT5G50915 AT5G50915 AT5G50915
fragaria_vesca FvH4_5g09140 FvH4_5g09140
malus_domestica MD06G1133200.v1.1 MD14G1149600.v1.1
prunus_persica Prupe.5G144400_v2.0.a1
pyrus_communis pycom06g12480 pycom14g12420
rosa_chinensis RchiOBHm_Chr7g0189021
rosa_laevigata RLG00000004638
rosa_roxburghii Rroxscaffold_3G00265840
rosa_rugosa Rorug06G0495700
rosa_samantha Rh7AG100200 Rh7BG102700 Rh7CG104100 Rh7DG102200
rosa_wichuraiana Rw7G008660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 289
Acc65I GGTACC 1 cut(s) 425
AccB1I GGYRCC 1 cut(s) 425
AciI CCGC 3 cut(s) 423, 888, 900
AclWI GGATC 1 cut(s) 186
AcsI RAATTY 1 cut(s) 129
AfaI GTAC 2 cut(s) 382, 427
AfiI CCNNNNNNNGG 2 cut(s) 348, 500
AgsI TTSAA 8 cut(s) 134, 150, 161, 287, 361, 727, 959, 1072
AjnI CCWGG 2 cut(s) 471, 616
AluBI AGCT 6 cut(s) 137, 155, 168, 186, 944, 993
AluI AGCT 6 cut(s) 137, 155, 168, 186, 944, 993
AlwI GGATC 1 cut(s) 186
Ama87I CYCGRG 1 cut(s) 123
AoxI GGCC 1 cut(s) 962
ApeKI GCWGC 6 cut(s) 5, 24, 165, 168, 601, 890
ApoI RAATTY 1 cut(s) 129
Asp718I GGTACC 1 cut(s) 425
AsuC2I CCSGG 2 cut(s) 124, 125
AsuHPI GGTGA 6 cut(s) 305, 487, 542, 577, 795, 898
AvaI CYCGRG 1 cut(s) 123
BanI GGYRCC 1 cut(s) 425
BbvCI CCTCAGC 1 cut(s) 438
BbvI GCAGC 6 cut(s) 17, 36, 155, 177, 588, 877
BccI CCATC 2 cut(s) 115, 820
BciT130I CCWGG 2 cut(s) 473, 618
BcnI CCSGG 2 cut(s) 124, 125
BfmI CTRYAG 1 cut(s) 537
BfuAI ACCTGC 1 cut(s) 289
BisI GCNGC 7 cut(s) 6, 25, 166, 169, 602, 888, 891
BlsI GCNGC 7 cut(s) 7, 26, 167, 170, 603, 889, 892
Bme1390I CCNGG 4 cut(s) 124, 125, 473, 618
BmeT110I CYCGRG 1 cut(s) 123
BmiI GGNNCC 1 cut(s) 427
BmrFI CCNGG 4 cut(s) 124, 125, 473, 618
BmsI GCATC 1 cut(s) 588
BplI GAGNNNNNCTC 2 cut(s) 689, 721
Bpu10I CCTNAGC 1 cut(s) 438
BpuMI CCSGG 2 cut(s) 124, 125
BsaJI CCNNGG 3 cut(s) 123, 375, 616
Bsc4I CCNNNNNNNGG 2 cut(s) 348, 500
Bse1I ACTGG 2 cut(s) 505, 640
BseBI CCWGG 2 cut(s) 473, 618
BseDI CCNNGG 3 cut(s) 123, 375, 616
BseGI GGATG 6 cut(s) 277, 353, 597, 661, 860, 1009
BseLI CCNNNNNNNGG 2 cut(s) 348, 500
BseMII CTCAG 3 cut(s) 102, 320, 429
BseNI ACTGG 2 cut(s) 505, 640
BseXI GCAGC 6 cut(s) 17, 36, 155, 177, 588, 877
BsgI GTGCAG 1 cut(s) 154
BshFI GGCC 1 cut(s) 964
BshNI GGYRCC 1 cut(s) 425
BsiHKCI CYCGRG 1 cut(s) 123
BsiSI CCGG 2 cut(s) 56, 124
BslFI GGGAC 2 cut(s) 599, 664
BslI CCNNNNNNNGG 2 cut(s) 348, 500
BsmFI GGGAC 2 cut(s) 599, 664
BsnI GGCC 1 cut(s) 964
BsoBI CYCGRG 1 cut(s) 123
Bsp143I GATC 3 cut(s) 95, 178, 1012
BspACI CCGC 3 cut(s) 423, 888, 900
BspANI GGCC 1 cut(s) 964
BspCNI CTCAG 3 cut(s) 103, 319, 430
BspLI GGNNCC 1 cut(s) 427
BspMI ACCTGC 1 cut(s) 289
BspPI GGATC 1 cut(s) 186
BspT107I GGYRCC 1 cut(s) 425
BsrI ACTGG 2 cut(s) 505, 640
BssECI CCNNGG 3 cut(s) 123, 375, 616
BssMI GATC 3 cut(s) 95, 178, 1012
BssT1I CCWWGG 1 cut(s) 375
Bst2UI CCWGG 2 cut(s) 473, 618
Bst4CI ACNGT 4 cut(s) 83, 265, 840, 986
Bst6I CTCTTC 2 cut(s) 326, 565
BstDEI CTNAG 3 cut(s) 111, 306, 438
BstF5I GGATG 6 cut(s) 277, 353, 597, 661, 860, 1009
BstKTI GATC 3 cut(s) 98, 181, 1015
BstMBI GATC 3 cut(s) 95, 178, 1012
BstMWI GCNNNNNNNGC 2 cut(s) 884, 899
BstNI CCWGG 2 cut(s) 473, 618
BstSCI CCNGG 4 cut(s) 122, 123, 471, 616
BstSFI CTRYAG 1 cut(s) 537
BstV1I GCAGC 6 cut(s) 17, 36, 155, 177, 588, 877
BsuRI GGCC 1 cut(s) 964
BtsCI GGATG 6 cut(s) 277, 353, 597, 661, 860, 1009
BtsI GCAGTG 2 cut(s) 873, 882
BtsIMutI CAGTG 4 cut(s) 707, 873, 882, 991
BveI ACCTGC 1 cut(s) 289
Cfr9I CCCGGG 1 cut(s) 123
CsiI ACCWGGT 1 cut(s) 471
Csp6I GTAC 2 cut(s) 381, 426
CspCI CAANNNNNGTGG 4 cut(s) 249, 284, 832, 867
CviAII CATG 5 cut(s) 512, 649, 757, 796, 1038
CviQI GTAC 2 cut(s) 381, 426
DdeI CTNAG 3 cut(s) 111, 306, 438
DpnI GATC 3 cut(s) 97, 180, 1014
DpnII GATC 3 cut(s) 95, 178, 1012
Eam1104I CTCTTC 2 cut(s) 326, 565
EarI CTCTTC 2 cut(s) 326, 565
Eco130I CCWWGG 1 cut(s) 375
Eco88I CYCGRG 1 cut(s) 123
EcoRII CCWGG 2 cut(s) 471, 616
EcoT14I CCWWGG 1 cut(s) 375
ErhI CCWWGG 1 cut(s) 375
FaeI CATG 5 cut(s) 515, 652, 760, 799, 1041
FaqI GGGAC 2 cut(s) 599, 664
FatI CATG 5 cut(s) 511, 648, 756, 795, 1037
Fnu4HI GCNGC 7 cut(s) 6, 25, 166, 169, 602, 888, 891
FokI GGATG 6 cut(s) 284, 340, 604, 668, 847, 1016
Fsp4HI GCNGC 7 cut(s) 6, 25, 166, 169, 602, 888, 891
GluI GCNGC 7 cut(s) 6, 25, 166, 169, 602, 888, 891
HaeIII GGCC 1 cut(s) 964
HapII CCGG 2 cut(s) 56, 124
Hin1II CATG 5 cut(s) 515, 652, 760, 799, 1041
HinfI GANTC 4 cut(s) 32, 254, 727, 800
HpaII CCGG 2 cut(s) 56, 124
HphI GGTGA 6 cut(s) 305, 487, 542, 577, 795, 898
Hpy166II GTNNAC 1 cut(s) 843
Hpy188I TCNGA 3 cut(s) 112, 309, 460
Hpy188III TCNNGA 4 cut(s) 93, 150, 342, 932
Hpy8I GTNNAC 1 cut(s) 843
HpyAV CCTTC 7 cut(s) 237, 367, 406, 427, 633, 913, 982
HpyCH4III ACNGT 4 cut(s) 83, 265, 840, 986
HpyCH4V TGCA 5 cut(s) 171, 557, 604, 820, 828
HpyF10VI GCNNNNNNNGC 2 cut(s) 884, 899
HpyF3I CTNAG 3 cut(s) 111, 306, 438
Hsp92II CATG 5 cut(s) 515, 652, 760, 799, 1041
KpnI GGTACC 1 cut(s) 429
Kzo9I GATC 3 cut(s) 95, 178, 1012
LmnI GCTCC 2 cut(s) 58, 191
Lsp1109I GCAGC 6 cut(s) 17, 36, 155, 177, 588, 877
LweI GCATC 1 cut(s) 588
MabI ACCWGGT 1 cut(s) 471
MaeIII GTNAC 8 cut(s) 83, 209, 232, 301, 313, 545, 623, 631
MalI GATC 3 cut(s) 97, 180, 1014
MboI GATC 3 cut(s) 95, 178, 1012
MboII GAAGA 6 cut(s) 343, 460, 500, 582, 607, 1022
MlyI GAGTC 2 cut(s) 26, 263
MmeI TCCRAC 1 cut(s) 521
MnlI CCTC 7 cut(s) 268, 433, 504, 519, 526, 582, 713
MseI TTAA 4 cut(s) 119, 453, 774, 1075
MspA1I CMGCKG 2 cut(s) 168, 890
MspI CCGG 2 cut(s) 56, 124
MspR9I CCNGG 4 cut(s) 124, 125, 473, 618
MvaI CCWGG 2 cut(s) 473, 618
MwoI GCNNNNNNNGC 2 cut(s) 884, 899
NciI CCSGG 2 cut(s) 124, 125
NdeII GATC 3 cut(s) 95, 178, 1012
NlaIII CATG 5 cut(s) 515, 652, 760, 799, 1041
NlaIV GGNNCC 1 cut(s) 427
NmuCI GTSAC 5 cut(s) 209, 232, 301, 545, 623
PfeI GAWTC 2 cut(s) 727, 800
PkrI GCNGC 7 cut(s) 7, 26, 167, 170, 603, 889, 892
PleI GAGTC 2 cut(s) 26, 262
PpsI GAGTC 2 cut(s) 26, 262
Psp6I CCWGG 2 cut(s) 471, 616
PspGI CCWGG 2 cut(s) 471, 616
PspN4I GGNNCC 1 cut(s) 427
PvuII CAGCTG 1 cut(s) 168
RsaI GTAC 2 cut(s) 382, 427
RsaNI GTAC 2 cut(s) 381, 426
SaqAI TTAA 4 cut(s) 119, 453, 774, 1075
SatI GCNGC 7 cut(s) 6, 25, 166, 169, 602, 888, 891
Sau3AI GATC 3 cut(s) 95, 178, 1012
SchI GAGTC 2 cut(s) 26, 263
ScrFI CCNGG 4 cut(s) 124, 125, 473, 618
SexAI ACCWGGT 1 cut(s) 471
SfaNI GCATC 1 cut(s) 588
SfcI CTRYAG 1 cut(s) 537
SmaI CCCGGG 1 cut(s) 125
SsiI CCGC 3 cut(s) 423, 888, 900
StyD4I CCNGG 4 cut(s) 122, 123, 471, 616
StyI CCWWGG 1 cut(s) 375
TaaI ACNGT 4 cut(s) 83, 265, 840, 986
TaqI TCGA 1 cut(s) 94
TauI GCSGC 1 cut(s) 890
TfiI GAWTC 2 cut(s) 727, 800
Tru1I TTAA 4 cut(s) 119, 453, 774, 1075
Tru9I TTAA 4 cut(s) 119, 453, 774, 1075
TscAI CASTG 4 cut(s) 714, 880, 889, 991
TseFI GTSAC 5 cut(s) 209, 232, 301, 545, 623
TseI GCWGC 6 cut(s) 5, 24, 165, 168, 601, 890
Tsp45I GTSAC 5 cut(s) 209, 232, 301, 545, 623
TspDTI ATGAA 4 cut(s) 500, 608, 672, 1019
TspMI CCCGGG 1 cut(s) 123
TspRI CASTG 4 cut(s) 714, 880, 889, 991
XapI RAATTY 1 cut(s) 129
XmaI CCCGGG 1 cut(s) 123
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.