MD06G1135600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0014047
Physical Location & Seq
Reverse (-)
28056120 .. 28057763
1644 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1135600.v1.1.491

Sequence Viewer

Length: 840 bp
ATGGGTGACCAAAATTCAAAGCTCACCCCAGAAGCCGAGGCTGTGCCTCCTAGAATCCTTTCCCTCTTGCAGAGACGCTTCGAGGAGATGAAGAGCCGGTCCAAGGCACGAAAGCTAAAAGCCGCTAGTACTCTCTCCAAGAAACAGCTACTTAAGGACTGTGCACAGGAGGAGGTTGATAACTCTCTCCTTCATTCGCCGCAGTGTTCACCAGAGTCTGACAAAATAACGTTTCCGAAGATACCACCAACTTTGGAGAACAAGTCCAAGGTTGCACCAATCTCGCAGGAGTGTGACAAAGGTACAAAACAGGTTGACGATGAGGACCAAAAGAAGGAGCAAAGTAAGAGAGATGGGACGGAGTGTAGTGTTGAAGATCATAAAGAGCCAAAGGGTGTGAATGTGTATATAGGAGGGGTTTTTGCAATGGAGGACACCACAGAGGGAGAGGAAGAGGACGAAGATAATGAAGATGGTACGCAGAGAAACAGAGAATTTTTCATGTGTCCCGCGTCACCAAGTTTTAAAATTTATTGCATGGAAGCTCTGGAAATTGAAAATGATAGCAGCAAGTACGACAGCACAACGGACGAGGATATCAAGCACAAGAAATCGCCAAGTGCTGCTTCTGTAGACTGCAGTGTTGATAGCCTAGCGTCAACGACTGATCACCACGATGGCCAGGTAACAAAGACAAAGAAGAAAGGAAGGAGAAGGAGAAGAGTACGAAATGGATTCGGAGTAAAGAATCTATTGCACATTAAAGGATGCTACTACCTAGGTTGCAGTGGTGGCCACGACAGAGCCACCTATCTGGCCGAGAAATCTGCAGCCACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

280

Amino Acids

31.14

Weight (kDa)

6.05

Isoelectric Point (pI)

63.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014047)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26934
fragaria_vesca FvH4_5g09010
malus_domestica MD06G1135600.v1.1 MD14G1150800.v1.1
prunus_persica Prupe.5G146000_v2.0.a1 Prupe.5G146000_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0189271
rosa_laevigata RLG00000004621
rosa_multiflora Rmu_sc0004318.1_g000023 Rmu_sc0007286.1_g000008
rosa_roxburghii Rroxscaffold_3G00265570
rosa_rugosa Rorug06G0496700
rosa_samantha Rh7AG101700 Rh7BG104300 Rh7CG105800 Rh7DG103500
rosa_wichuraiana Rw7G008790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 633
AccII CGCG 1 cut(s) 512
AciI CCGC 3 cut(s) 123, 200, 510
AclI AACGTT 1 cut(s) 230
AcoI YGGCCR 3 cut(s) 679, 793, 816
AcsI RAATTY 3 cut(s) 13, 494, 528
AfaI GTAC 5 cut(s) 130, 304, 478, 575, 726
AfiI CCNNNNNNNGG 2 cut(s) 103, 334
AflII CTTAAG 1 cut(s) 152
AgsI TTSAA 3 cut(s) 18, 374, 557
AjnI CCWGG 1 cut(s) 681
AjuI GAANNNNNNNTTGG 2 cut(s) 610, 642
AluBI AGCT 4 cut(s) 22, 115, 148, 545
AluI AGCT 4 cut(s) 22, 115, 148, 545
Alw21I GWGCWC 1 cut(s) 166
Alw26I GTCTC 1 cut(s) 67
Alw44I GTGCAC 1 cut(s) 162
AlwNI CAGNNNCTG 1 cut(s) 218
AoxI GGCC 3 cut(s) 679, 793, 816
ApaLI GTGCAC 1 cut(s) 162
ApeKI GCWGC 3 cut(s) 567, 623, 830
ApoI RAATTY 3 cut(s) 13, 494, 528
Asp700I GAANNNNTTC 1 cut(s) 58
AspA2I CCTAGG 1 cut(s) 778
AspS9I GGNCC 2 cut(s) 99, 325
AsuHPI GGTGA 5 cut(s) 16, 17, 201, 507, 662
AvaII GGWCC 2 cut(s) 99, 325
AvrII CCTAGG 1 cut(s) 778
BaeGI GKGCMC 1 cut(s) 166
BalI TGGCCA 2 cut(s) 681, 795
Bbv12I GWGCWC 1 cut(s) 166
BbvI GCAGC 2 cut(s) 579, 610
BccI CCATC 3 cut(s) 347, 467, 671
BcgI CGANNNNNNTGC 3 cut(s) 264, 298, 809
BciT130I CCWGG 1 cut(s) 683
BclI TGATCA 1 cut(s) 667
BcoDI GTCTC 1 cut(s) 67
BfaI CTAG 4 cut(s) 51, 126, 653, 779
BfmI CTRYAG 3 cut(s) 630, 637, 828
BfrI CTTAAG 1 cut(s) 152
BisI GCNGC 5 cut(s) 123, 200, 568, 624, 831
BlnI CCTAGG 1 cut(s) 778
BlsI GCNGC 5 cut(s) 124, 201, 569, 625, 832
BmcAI AGTACT 1 cut(s) 130
Bme1390I CCNGG 1 cut(s) 683
Bme18I GGWCC 2 cut(s) 99, 325
BmgT120I GGNCC 2 cut(s) 99, 325
BmrFI CCNGG 1 cut(s) 683
BmsI GCATC 1 cut(s) 758
BsaJI CCNNGG 4 cut(s) 36, 102, 267, 778
BsaXI ACNNNNNCTCC 2 cut(s) 248, 278
Bsc4I CCNNNNNNNGG 2 cut(s) 103, 334
Bse118I RCCGGY 1 cut(s) 96
Bse3DI GCAATG 1 cut(s) 432
BseBI CCWGG 1 cut(s) 683
BseDI CCNNGG 4 cut(s) 36, 102, 267, 778
BseGI GGATG 1 cut(s) 773
BseLI CCNNNNNNNGG 2 cut(s) 103, 334
BseMI GCAATG 1 cut(s) 432
BseRI GAGGAG 2 cut(s) 98, 185
BseSI GKGCMC 1 cut(s) 166
BseXI GCAGC 2 cut(s) 579, 610
Bsh1236I CGCG 1 cut(s) 512
BshFI GGCC 3 cut(s) 681, 795, 818
BsiHKAI GWGCWC 1 cut(s) 166
BsiSI CCGG 1 cut(s) 97
BslFI GGGAC 2 cut(s) 370, 492
BslI CCNNNNNNNGG 2 cut(s) 103, 334
BsmAI GTCTC 1 cut(s) 67
BsmBI CGTCTC 1 cut(s) 67
BsmFI GGGAC 2 cut(s) 370, 492
BsnI GGCC 3 cut(s) 681, 795, 818
Bsp1286I GDGCHC 1 cut(s) 166
Bsp143I GATC 2 cut(s) 376, 667
BspACI CCGC 3 cut(s) 123, 200, 510
BspANI GGCC 3 cut(s) 681, 795, 818
BspFNI CGCG 1 cut(s) 512
BspMAI CTGCAG 2 cut(s) 641, 832
BspQI GCTCTTC 1 cut(s) 86
BspTI CTTAAG 1 cut(s) 152
BsrDI GCAATG 1 cut(s) 432
BsrFI RCCGGY 1 cut(s) 96
BssAI RCCGGY 1 cut(s) 96
BssECI CCNNGG 4 cut(s) 36, 102, 267, 778
BssMI GATC 2 cut(s) 376, 667
BssT1I CCWWGG 3 cut(s) 102, 267, 778
Bst2UI CCWGG 1 cut(s) 683
Bst4CI ACNGT 1 cut(s) 161
Bst6I CTCTTC 3 cut(s) 86, 447, 715
BstAFI CTTAAG 1 cut(s) 152
BstEII GGTNACC 1 cut(s) 5
BstF5I GGATG 1 cut(s) 773
BstFNI CGCG 1 cut(s) 512
BstKTI GATC 2 cut(s) 379, 670
BstMAI GTCTC 1 cut(s) 67
BstMBI GATC 2 cut(s) 376, 667
BstMWI GCNNNNNNNGC 1 cut(s) 792
BstNI CCWGG 1 cut(s) 683
BstPI GGTNACC 1 cut(s) 5
BstSCI CCNGG 1 cut(s) 681
BstSFI CTRYAG 3 cut(s) 630, 637, 828
BstSLI GKGCMC 1 cut(s) 166
BstUI CGCG 1 cut(s) 512
BstV1I GCAGC 2 cut(s) 579, 610
BstXI CCANNNNNNTGG 1 cut(s) 814
BsuRI GGCC 3 cut(s) 681, 795, 818
BtsCI GGATG 1 cut(s) 773
BtsI GCAGTG 3 cut(s) 209, 646, 793
BtsIMutI CAGTG 3 cut(s) 209, 646, 793
CaiI CAGNNNCTG 1 cut(s) 218
Cfr10I RCCGGY 1 cut(s) 96
Cfr13I GGNCC 2 cut(s) 99, 325
CseI GACGC 3 cut(s) 84, 501, 645
Csp6I GTAC 5 cut(s) 129, 303, 477, 574, 725
CspCI CAANNNNNGTGG 2 cut(s) 234, 269
CviAII CATG 2 cut(s) 502, 538
CviQI GTAC 5 cut(s) 129, 303, 477, 574, 725
DpnI GATC 2 cut(s) 378, 669
DpnII GATC 2 cut(s) 376, 667
DraI TTTAAA 1 cut(s) 526
EaeI YGGCCR 3 cut(s) 679, 793, 816
Eam1104I CTCTTC 3 cut(s) 86, 447, 715
EarI CTCTTC 3 cut(s) 86, 447, 715
Eco130I CCWWGG 3 cut(s) 102, 267, 778
Eco32I GATATC 1 cut(s) 598
Eco47I GGWCC 2 cut(s) 99, 325
Eco91I GGTNACC 1 cut(s) 5
EcoO65I GGTNACC 1 cut(s) 5
EcoRII CCWGG 1 cut(s) 681
EcoRV GATATC 1 cut(s) 598
EcoT14I CCWWGG 3 cut(s) 102, 267, 778
ErhI CCWWGG 3 cut(s) 102, 267, 778
Esp3I CGTCTC 1 cut(s) 67
FaeI CATG 2 cut(s) 505, 541
FaiI YATR 5 cut(s) 381, 408, 410, 503, 539
FalI AAGNNNNNCTT 2 cut(s) 610, 642
FaqI GGGAC 2 cut(s) 370, 492
FatI CATG 2 cut(s) 501, 537
FauI CCCGC 1 cut(s) 517
FbaI TGATCA 1 cut(s) 667
FblI GTMKAC 1 cut(s) 633
Fnu4HI GCNGC 5 cut(s) 123, 200, 568, 624, 831
FokI GGATG 1 cut(s) 780
Fsp4HI GCNGC 5 cut(s) 123, 200, 568, 624, 831
FspBI CTAG 4 cut(s) 51, 126, 653, 779
GluI GCNGC 5 cut(s) 123, 200, 568, 624, 831
HaeIII GGCC 3 cut(s) 681, 795, 818
HapII CCGG 1 cut(s) 97
HgaI GACGC 3 cut(s) 84, 501, 645
Hin1II CATG 2 cut(s) 505, 541
HincII GTYRAC 2 cut(s) 316, 660
HindII GTYRAC 2 cut(s) 316, 660
HinfI GANTC 4 cut(s) 54, 215, 735, 748
HpaII CCGG 1 cut(s) 97
HphI GGTGA 5 cut(s) 16, 17, 201, 507, 662
Hpy166II GTNNAC 5 cut(s) 164, 209, 316, 634, 660
Hpy188I TCNGA 3 cut(s) 220, 237, 740
Hpy188III TCNNGA 1 cut(s) 548
Hpy8I GTNNAC 5 cut(s) 164, 209, 316, 634, 660
HpyAV CCTTC 4 cut(s) 200, 328, 702, 708
HpyCH4III ACNGT 1 cut(s) 161
HpyCH4IV ACGT 1 cut(s) 230
HpyCH4V TGCA 9 cut(s) 70, 164, 275, 425, 537, 639, 757, 786, 830
HpyF10VI GCNNNNNNNGC 1 cut(s) 792
HpySE526I ACGT 1 cut(s) 230
Hsp92II CATG 2 cut(s) 505, 541
Ksp22I TGATCA 1 cut(s) 667
Kzo9I GATC 2 cut(s) 376, 667
LguI GCTCTTC 1 cut(s) 86
LmnI GCTCC 1 cut(s) 337
Lsp1109I GCAGC 2 cut(s) 579, 610
LweI GCATC 1 cut(s) 758
MaeI CTAG 4 cut(s) 51, 126, 653, 779
MaeII ACGT 1 cut(s) 230
MaeIII GTNAC 4 cut(s) 5, 293, 513, 685
MalI GATC 2 cut(s) 378, 669
MboI GATC 2 cut(s) 376, 667
MboII GAAGA 8 cut(s) 103, 250, 386, 464, 473, 482, 712, 732
MhlI GDGCHC 1 cut(s) 166
MlsI TGGCCA 2 cut(s) 681, 795
MluCI AATT 4 cut(s) 13, 494, 528, 552
MluNI TGGCCA 2 cut(s) 681, 795
MlyI GAGTC 1 cut(s) 224
Mox20I TGGCCA 2 cut(s) 681, 795
MroXI GAANNNNTTC 1 cut(s) 58
MscI TGGCCA 2 cut(s) 681, 795
MseI TTAA 3 cut(s) 153, 525, 762
MslI CAYNNNNRTG 1 cut(s) 675
Msp20I TGGCCA 2 cut(s) 681, 795
MspCI CTTAAG 1 cut(s) 152
MspI CCGG 1 cut(s) 97
MspR9I CCNGG 1 cut(s) 683
MvaI CCWGG 1 cut(s) 683
MvnI CGCG 1 cut(s) 512
MwoI GCNNNNNNNGC 1 cut(s) 792
NdeII GATC 2 cut(s) 376, 667
NlaIII CATG 2 cut(s) 505, 541
NmeAIII GCCGAG 1 cut(s) 61
NmuCI GTSAC 3 cut(s) 5, 293, 513
PciSI GCTCTTC 1 cut(s) 86
PdmI GAANNNNTTC 1 cut(s) 58
PfeI GAWTC 3 cut(s) 54, 735, 748
PkrI GCNGC 5 cut(s) 124, 201, 569, 625, 832
PleI GAGTC 1 cut(s) 223
PpsI GAGTC 1 cut(s) 223
Psp1406I AACGTT 1 cut(s) 230
Psp6I CCWGG 1 cut(s) 681
PspEI GGTNACC 1 cut(s) 5
PspGI CCWGG 1 cut(s) 681
PspPI GGNCC 2 cut(s) 99, 325
PstI CTGCAG 2 cut(s) 641, 832
PstNI CAGNNNCTG 1 cut(s) 218
RsaI GTAC 5 cut(s) 130, 304, 478, 575, 726
RsaNI GTAC 5 cut(s) 129, 303, 477, 574, 725
RseI CAYNNNNRTG 1 cut(s) 675
SapI GCTCTTC 1 cut(s) 86
SaqAI TTAA 3 cut(s) 153, 525, 762
SatI GCNGC 5 cut(s) 123, 200, 568, 624, 831
Sau3AI GATC 2 cut(s) 376, 667
Sau96I GGNCC 2 cut(s) 99, 325
ScaI AGTACT 1 cut(s) 130
SchI GAGTC 1 cut(s) 224
ScrFI CCNGG 1 cut(s) 683
SduI GDGCHC 1 cut(s) 166
SfaNI GCATC 1 cut(s) 758
SfcI CTRYAG 3 cut(s) 630, 637, 828
SinI GGWCC 2 cut(s) 99, 325
SmiMI CAYNNNNRTG 1 cut(s) 675
SmlI CTYRAG 1 cut(s) 152
SmoI CTYRAG 1 cut(s) 152
Sse9I AATT 4 cut(s) 13, 494, 528, 552
SsiI CCGC 3 cut(s) 123, 200, 510
SspMI CTAG 4 cut(s) 51, 126, 653, 779
StyD4I CCNGG 1 cut(s) 681
StyI CCWWGG 3 cut(s) 102, 267, 778
TaaI ACNGT 1 cut(s) 161
TaiI ACGT 1 cut(s) 233
TaqI TCGA 1 cut(s) 81
TasI AATT 4 cut(s) 13, 494, 528, 552
TatI WGTACW 1 cut(s) 128
TauI GCSGC 2 cut(s) 125, 202
TfiI GAWTC 3 cut(s) 54, 735, 748
Tru1I TTAA 3 cut(s) 153, 525, 762
Tru9I TTAA 3 cut(s) 153, 525, 762
TscAI CASTG 3 cut(s) 209, 646, 793
TseFI GTSAC 3 cut(s) 5, 293, 513
TseI GCWGC 3 cut(s) 567, 623, 830
Tsp45I GTSAC 3 cut(s) 5, 293, 513
TspDTI ATGAA 4 cut(s) 104, 182, 483, 490
TspGWI ACGGA 2 cut(s) 374, 602
TspRI CASTG 3 cut(s) 209, 646, 793
Vha464I CTTAAG 1 cut(s) 152
VneI GTGCAC 1 cut(s) 162
VpaK11BI GGWCC 2 cut(s) 99, 325
XapI RAATTY 3 cut(s) 13, 494, 528
XmaJI CCTAGG 1 cut(s) 778
XmiI GTMKAC 1 cut(s) 633
XmnI GAANNNNTTC 1 cut(s) 58
XspI CTAG 4 cut(s) 51, 126, 653, 779
ZrmI AGTACT 1 cut(s) 130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.