MD06G1143900.v1.1

Protein ROOT INITIATION DEFECTIVE 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0012617
Physical Location & Seq
Forward (+)
28735013 .. 28738421
3409 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1143900.v1.1.491

Sequence Viewer

Length: 1455 bp
ATGGGAAGTGGTGGCGGTGAGGCGGTGGTTGTGTGCAGTGACAAGAGGATGGGAACAGGGATAACTATATGGAACATAGACACAGGGGATCGACTCCTTCACATACCAACCTGCGCTTCTCCCGCTCGTGGTCTTGTGTGCTTGAGAAACCAGTTCCTTGTAGCTGCTCAGATTCACAAACCTGGCTCCGTTGGCGGCGGCGCCATCTCTATGTGGAAGTTGAACAAGCACTTTCTTATGCATATCATATTTGGTTTGTTGTACGCAACATTTTCGCAGCCTCAGAAGCCTATTCAGAGCTACCCGCTGGAGGCAATTGGGCCACTTTCTTGCACAAAGGATGGTCTATATCTTGCAGGTGGCGCTCTTTCTGGAAACACTTACCTTTGGGAGGTTACTAACGGAAAATTGCTCAAAACTTGGCATGGTCATGACAAATCTTTGAGCTGTATGTTGTTTTCCGATGATGGTTCTCTTCTTGTTTCTGGTTCCGATGATGGGATGATATGTGTGTGGAACTTGATTAGTTTGCTGGACGTGGAAAATGTTGAAAGCCTTCCGTCACCATTACATTATTCAATGGAGCATAAATCTTCTATAACCGGCCTGTTAACTACGTCAGGCATCTCAAGTCCGGTGTTAATATCAAGCTCACTTGATGGCTCATACAAGGTTTGGGATCTAGTCTTGGGAAACCTCATGCATACTCAAGTTTATCCACTAGGGATAACTGCAGTTGCTCTTCACCCAACGAAGCAGTTAATTTTCTCCGGAAGTATAGACGGAAGCATTTTTGTGAACAAACTTGAAATCGGACTGGTAGAGGATTATTTGGTTGGTGCTGAAGATCACTCACCTGTGCTGAAAGAACACAATGGATCTGTTACCGCATTAACCTTCAGCAAATCGGGTCTGATATCCGCATCTGAGGACTGCACCATCTGCATTTGGGATATCACCAGTTTTGAAATCATCCGAAGATTCAACCATCAGAAAGGGCCTGTGACTAATCTGTCAGTGATTCCACAGTCCTCGTTGCTTCCAGTGTCAAACCATCGTAAACTTTCTAATGCACTTGGTGTTTCCATCCTCGACAAGTATCCGCAACCTGCAAACTCATCGGAGGAGAAAATCACTCTTTTTCCATCATGCCAACCCTCCATGAATTTCCAAACCTCGGATTCATGCCAACAAATATATGACATGAAGAAAGAGGGCACTGTTGCAGCAATGGAGATGAAATTGGAAACATGTCTAGAGGACCGAATGAGGGCTACAAAAATGGCAAAGCATGTGATGGAGATGAACAGGCATTTGCAATCACGTTTGCTAGACTTGATGGAGAGCAGATTATCGCAGCCCAAGTCTAGCGAAATCGATTCACCTTCGATGAAAAAGAGCAAGACTGTCATGCTTGAAAACCCCCCATTGCAGCAGGAGGAGGAGCAGCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0007275 GO:0007368 GO:0007389 GO:0008150 GO:0009799 GO:0009855 GO:0032501 GO:0032502 GO:0048856 GO:0070121
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

485

Amino Acids

52.98

Weight (kDa)

6.15

Isoelectric Point (pI)

53.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 105 - 228 1.2e-08 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 109 - 176 2.3e-09 THOC3 beta-propeller domain
Beta-prop_EML PF23409 109 - 173 1.9e-06 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR5 PF25175 109 - 287 4.7e-19 WDR5 beta-propeller domain
Beta-prop_EML_2 PF23414 109 - 174 5.4e-06 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 109 - 266 1.2e-19 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 114 - 181 7.7e-08 Prp19 WD40 domain
Beta-prop_WDR36-Utp21_1st PF25171 116 - 226 1.7e-06 WDR36/Utp21 first beta-propeller
Beta-prop_WDR3_2nd PF25172 121 - 347 7.2e-13 WDR3 second beta-propeller domain
NBCH_WD40 PF20426 127 - 347 6.3e-07 Neurobeachin beta propeller domain
WD40 PF00400 135 - 173 2.4e-10 WD domain, G-beta repeat
Beta-prop_TEP1_2nd PF25047 140 - 235 1.3e-06 TEP-1 second beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 195 - 350 4.1e-11 WDR36/Utp21 second beta-propeller domain
Beta-prop_EML_2 PF23414 211 - 348 7.6e-07 Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st PF24817 212 - 345 1.2e-07 WDHD1 first WD40 domain
Beta-prop_THOC3 PF25174 213 - 351 5.2e-10 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 214 - 344 7.3e-06 CDC20/Fizzy WD40 domain
WD40_Prp19 PF24814 242 - 412 6.6e-10 Prp19 WD40 domain
WDR55 PF24796 243 - 343 4.9e-07 WDR55
Beta-prop_THOC3 PF25174 243 - 403 7.4e-09 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 243 - 347 3.8e-11 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 288 - 347 8e-06 WDR5 beta-propeller domain
Beta-prop_TEP1_C PF25048 291 - 344 4.4e-06 TEP-1 C-terminal beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 347
AasI GACNNNNNNGTC 1 cut(s) 1012
Acc36I ACCTGC 3 cut(s) 119, 347, 1117
AccB1I GGYRCC 1 cut(s) 200
AccBSI CCGCTC 1 cut(s) 125
AccIII TCCGGA 1 cut(s) 770
AciI CCGC 9 cut(s) 15, 23, 123, 195, 198, 305, 888, 921, 1103
AclWI GGATC 3 cut(s) 96, 687, 886
AcsI RAATTY 1 cut(s) 1165
AcuI CTGAAG 2 cut(s) 864, 883
AcyI GRCGYC 1 cut(s) 201
AdeI CACNNNGTG 1 cut(s) 1079
AfaI GTAC 1 cut(s) 263
AfiI CCNNNNNNNGG 6 cut(s) 128, 310, 391, 498, 1177, 1270
AflIII ACRYGT 1 cut(s) 1250
AgsI TTSAA 7 cut(s) 223, 551, 579, 809, 968, 985, 1418
AjiI CACGTC 1 cut(s) 538
AjnI CCWGG 1 cut(s) 181
AjuI GAANNNNNNNTTGG 2 cut(s) 100, 132
AluBI AGCT 4 cut(s) 164, 300, 447, 651
AluI AGCT 4 cut(s) 164, 300, 447, 651
AlwI GGATC 3 cut(s) 96, 687, 886
Aor13HI TCCGGA 1 cut(s) 770
AoxI GGCC 3 cut(s) 320, 604, 998
ApeKI GCWGC 6 cut(s) 164, 277, 1226, 1357, 1432, 1447
ApoI RAATTY 1 cut(s) 1165
Asp700I GAANNNNTTC 1 cut(s) 555
AspLEI GCGC 3 cut(s) 116, 203, 365
AspS9I GGNCC 3 cut(s) 320, 998, 1261
AsuHPI GGTGA 6 cut(s) 29, 555, 737, 846, 949, 1374
AvaII GGWCC 1 cut(s) 1261
BaeGI GKGCMC 1 cut(s) 1220
BanI GGYRCC 1 cut(s) 200
BauI CACGAG 1 cut(s) 126
BbvI GCAGC 5 cut(s) 151, 289, 1238, 1369, 1444
BcgI CGANNNNNNTGC 4 cut(s) 255, 289, 1101, 1135
BciT130I CCWGG 1 cut(s) 183
BciVI GTATCC 1 cut(s) 1110
BfaI CTAG 5 cut(s) 683, 722, 1256, 1331, 1368
BfmI CTRYAG 1 cut(s) 732
BfoI RGCGCY 2 cut(s) 204, 366
BfuAI ACCTGC 3 cut(s) 119, 347, 1117
BfuI GTATCC 1 cut(s) 1110
BisI GCNGC 8 cut(s) 165, 196, 199, 278, 1227, 1358, 1433, 1448
BlsI GCNGC 8 cut(s) 166, 197, 200, 279, 1228, 1359, 1434, 1449
Bme1390I CCNGG 1 cut(s) 183
Bme18I GGWCC 1 cut(s) 1261
BmgBI CACGTC 1 cut(s) 538
BmgT120I GGNCC 3 cut(s) 320, 998, 1261
BmiI GGNNCC 3 cut(s) 187, 202, 490
BmrFI CCNGG 1 cut(s) 183
BmsI GCATC 2 cut(s) 633, 932
BpmI CTGGAG 1 cut(s) 329
BpuEI CTTGAG 3 cut(s) 163, 613, 693
Bsa29I ATCGAT 1 cut(s) 1377
BsaHI GRCGYC 1 cut(s) 201
BsaJI CCNNGG 1 cut(s) 1176
BsaWI WCCGGW 2 cut(s) 634, 770
Bsc4I CCNNNNNNNGG 6 cut(s) 128, 310, 391, 498, 1177, 1270
Bse118I RCCGGY 1 cut(s) 602
Bse1I ACTGG 4 cut(s) 151, 822, 960, 1043
Bse3DI GCAATG 2 cut(s) 1236, 1427
BseAI TCCGGA 1 cut(s) 770
BseBI CCWGG 1 cut(s) 183
BseCI ATCGAT 1 cut(s) 1377
BseDI CCNNGG 1 cut(s) 1176
BseGI GGATG 5 cut(s) 54, 346, 507, 972, 1086
BseLI CCNNNNNNNGG 6 cut(s) 128, 310, 391, 498, 1177, 1270
BseMI GCAATG 2 cut(s) 1236, 1427
BseMII CTCAG 3 cut(s) 182, 296, 918
BseNI ACTGG 4 cut(s) 151, 822, 960, 1043
BseRI GAGGAG 2 cut(s) 1139, 1454
BseSI GKGCMC 1 cut(s) 1220
BseXI GCAGC 5 cut(s) 151, 289, 1238, 1369, 1444
BsgI GTGCAG 2 cut(s) 55, 919
BshFI GGCC 3 cut(s) 322, 606, 1000
BshNI GGYRCC 1 cut(s) 200
BshVI ATCGAT 1 cut(s) 1377
BsiSI CCGG 3 cut(s) 603, 635, 771
BslI CCNNNNNNNGG 6 cut(s) 128, 310, 391, 498, 1177, 1270
BsnI GGCC 3 cut(s) 322, 606, 1000
Bsp1286I GDGCHC 1 cut(s) 1220
Bsp13I TCCGGA 1 cut(s) 770
Bsp143I GATC 4 cut(s) 88, 679, 847, 878
BspACI CCGC 9 cut(s) 15, 23, 123, 195, 198, 305, 888, 921, 1103
BspANI GGCC 3 cut(s) 322, 606, 1000
BspCNI CTCAG 3 cut(s) 181, 295, 919
BspDI ATCGAT 1 cut(s) 1377
BspEI TCCGGA 1 cut(s) 770
BspHI TCATGA 1 cut(s) 430
BspLI GGNNCC 3 cut(s) 187, 202, 490
BspMAI CTGCAG 1 cut(s) 736
BspMI ACCTGC 3 cut(s) 119, 347, 1117
BspPI GGATC 3 cut(s) 96, 687, 886
BspQI GCTCTTC 1 cut(s) 747
BspT107I GGYRCC 1 cut(s) 200
BsrBI CCGCTC 1 cut(s) 125
BsrDI GCAATG 2 cut(s) 1236, 1427
BsrFI RCCGGY 1 cut(s) 602
BsrI ACTGG 4 cut(s) 151, 822, 960, 1043
BssAI RCCGGY 1 cut(s) 602
BssECI CCNNGG 1 cut(s) 1176
BssMI GATC 4 cut(s) 88, 679, 847, 878
BssNI GRCGYC 1 cut(s) 201
BssSI CACGAG 1 cut(s) 126
Bst2BI CACGAG 1 cut(s) 126
Bst2UI CCWGG 1 cut(s) 183
Bst4CI ACNGT 3 cut(s) 1029, 1222, 1408
Bst6I CTCTTC 2 cut(s) 480, 747
BstACI GRCGYC 1 cut(s) 201
BstAPI GCANNNNNTGC 1 cut(s) 942
BstDEI CTNAG 3 cut(s) 168, 282, 927
BstENI CCTNNNNNAGG 1 cut(s) 389
BstF5I GGATG 5 cut(s) 54, 346, 507, 972, 1086
BstH2I RGCGCY 2 cut(s) 204, 366
BstHHI GCGC 3 cut(s) 116, 203, 365
BstKTI GATC 4 cut(s) 91, 682, 850, 881
BstMBI GATC 4 cut(s) 88, 679, 847, 878
BstMWI GCNNNNNNNGC 5 cut(s) 122, 192, 286, 362, 942
BstNI CCWGG 1 cut(s) 183
BstNSI RCATGY 2 cut(s) 1254, 1295
BstSCI CCNGG 1 cut(s) 181
BstSFI CTRYAG 1 cut(s) 732
BstSLI GKGCMC 1 cut(s) 1220
BstV1I GCAGC 5 cut(s) 151, 289, 1238, 1369, 1444
BstX2I RGATCY 2 cut(s) 679, 878
BstYI RGATCY 2 cut(s) 679, 878
Bsu15I ATCGAT 1 cut(s) 1377
BsuI GTATCC 1 cut(s) 1110
BsuRI GGCC 3 cut(s) 322, 606, 1000
BsuTUI ATCGAT 1 cut(s) 1377
BtrI CACGTC 1 cut(s) 538
BtsCI GGATG 5 cut(s) 54, 346, 507, 972, 1086
BtsI GCAGTG 1 cut(s) 43
BtsIMutI CAGTG 4 cut(s) 43, 1023, 1050, 1218
BveI ACCTGC 3 cut(s) 119, 347, 1117
CciI TCATGA 1 cut(s) 430
CfoI GCGC 3 cut(s) 116, 203, 365
Cfr10I RCCGGY 1 cut(s) 602
Cfr13I GGNCC 3 cut(s) 320, 998, 1261
ClaI ATCGAT 1 cut(s) 1377
Csp6I GTAC 1 cut(s) 262
CviQI GTAC 1 cut(s) 262
DdeI CTNAG 3 cut(s) 168, 282, 927
DinI GGCGCC 1 cut(s) 202
DpnI GATC 4 cut(s) 90, 681, 849, 880
DpnII GATC 4 cut(s) 88, 679, 847, 878
DraIII CACNNNGTG 1 cut(s) 1079
DrdI GACNNNNNNGTC 1 cut(s) 1012
DseDI GACNNNNNNGTC 1 cut(s) 1012
Eam1104I CTCTTC 2 cut(s) 480, 747
EarI CTCTTC 2 cut(s) 480, 747
Eco32I GATATC 2 cut(s) 918, 955
Eco47I GGWCC 1 cut(s) 1261
Eco57I CTGAAG 2 cut(s) 864, 883
EcoNI CCTNNNNNAGG 1 cut(s) 389
EcoO109I RGGNCCY 1 cut(s) 998
EcoRII CCWGG 1 cut(s) 181
EcoRV GATATC 2 cut(s) 918, 955
EcoT22I ATGCAT 2 cut(s) 243, 705
EgeI GGCGCC 1 cut(s) 202
EheI GGCGCC 1 cut(s) 202
FauI CCCGC 2 cut(s) 130, 312
Fnu4HI GCNGC 8 cut(s) 165, 196, 199, 278, 1227, 1358, 1433, 1448
FokI GGATG 5 cut(s) 61, 353, 514, 959, 1073
Fsp4HI GCNGC 8 cut(s) 165, 196, 199, 278, 1227, 1358, 1433, 1448
FspBI CTAG 5 cut(s) 683, 722, 1256, 1331, 1368
GlaI GCGC 3 cut(s) 115, 202, 364
GluI GCNGC 8 cut(s) 165, 196, 199, 278, 1227, 1358, 1433, 1448
GsuI CTGGAG 1 cut(s) 329
HaeII RGCGCY 2 cut(s) 204, 366
HaeIII GGCC 3 cut(s) 322, 606, 1000
HapII CCGG 3 cut(s) 603, 635, 771
HhaI GCGC 3 cut(s) 116, 203, 365
Hin1I GRCGYC 1 cut(s) 201
Hin6I GCGC 3 cut(s) 114, 201, 363
HinP1I GCGC 3 cut(s) 114, 201, 363
HincII GTYRAC 1 cut(s) 612
HindII GTYRAC 1 cut(s) 612
HinfI GANTC 6 cut(s) 93, 172, 981, 1021, 1181, 1379
HpaI GTTAAC 1 cut(s) 612
HpaII CCGG 3 cut(s) 603, 635, 771
HphI GGTGA 6 cut(s) 29, 555, 737, 846, 949, 1374
Hpy166II GTNNAC 3 cut(s) 612, 799, 1061
Hpy188III TCNNGA 4 cut(s) 372, 431, 771, 1256
Hpy8I GTNNAC 3 cut(s) 612, 799, 1061
HpyAV CCTTC 4 cut(s) 107, 566, 907, 1395
HpyCH4III ACNGT 3 cut(s) 1029, 1222, 1408
HpyCH4IV ACGT 3 cut(s) 537, 617, 1324
HpyF10VI GCNNNNNNNGC 5 cut(s) 122, 192, 286, 362, 942
HpyF3I CTNAG 3 cut(s) 168, 282, 927
HpySE526I ACGT 3 cut(s) 537, 617, 1324
Hsp92I GRCGYC 1 cut(s) 201
HspAI GCGC 3 cut(s) 114, 201, 363
KasI GGCGCC 1 cut(s) 200
Kpn2I TCCGGA 1 cut(s) 770
KspAI GTTAAC 1 cut(s) 612
Kzo9I GATC 4 cut(s) 88, 679, 847, 878
LguI GCTCTTC 1 cut(s) 747
LmnI GCTCC 3 cut(s) 191, 583, 1444
Lsp1109I GCAGC 5 cut(s) 151, 289, 1238, 1369, 1444
LweI GCATC 2 cut(s) 633, 932
MaeI CTAG 5 cut(s) 683, 722, 1256, 1331, 1368
MaeII ACGT 3 cut(s) 537, 617, 1324
MaeIII GTNAC 5 cut(s) 38, 394, 561, 883, 1003
MalI GATC 4 cut(s) 90, 681, 849, 880
MbiI CCGCTC 1 cut(s) 125
MboI GATC 4 cut(s) 88, 679, 847, 878
MboII GAAGA 6 cut(s) 467, 585, 734, 857, 990, 1219
MfeI CAATTG 1 cut(s) 315
MflI RGATCY 2 cut(s) 679, 878
MhlI GDGCHC 1 cut(s) 1220
MluCI AATT 5 cut(s) 315, 407, 762, 1165, 1241
Mly113I GGCGCC 1 cut(s) 201
MlyI GAGTC 1 cut(s) 87
Mph1103I ATGCAT 2 cut(s) 243, 705
MroI TCCGGA 1 cut(s) 770
MroXI GAANNNNTTC 1 cut(s) 555
MseI TTAA 4 cut(s) 611, 641, 761, 893
MslI CAYNNNNRTG 3 cut(s) 209, 429, 794
MspA1I CMGCKG 1 cut(s) 307
MspI CCGG 3 cut(s) 603, 635, 771
MspR9I CCNGG 1 cut(s) 183
MunI CAATTG 1 cut(s) 315
MvaI CCWGG 1 cut(s) 183
MwoI GCNNNNNNNGC 5 cut(s) 122, 192, 286, 362, 942
NarI GGCGCC 1 cut(s) 201
NdeII GATC 4 cut(s) 88, 679, 847, 878
NlaIV GGNNCC 3 cut(s) 187, 202, 490
NmuCI GTSAC 3 cut(s) 38, 561, 1003
NsiI ATGCAT 2 cut(s) 243, 705
NspI RCATGY 2 cut(s) 1254, 1295
PagI TCATGA 1 cut(s) 430
PaqCI CACCTGC 1 cut(s) 347
PciI ACATGT 1 cut(s) 1250
PciSI GCTCTTC 1 cut(s) 747
PdmI GAANNNNTTC 1 cut(s) 555
PfeI GAWTC 5 cut(s) 172, 981, 1021, 1181, 1379
PkrI GCNGC 8 cut(s) 166, 197, 200, 279, 1228, 1359, 1434, 1449
PleI GAGTC 1 cut(s) 87
PluTI GGCGCC 1 cut(s) 204
PpsI GAGTC 1 cut(s) 87
PscI ACATGT 1 cut(s) 1250
Psp6I CCWGG 1 cut(s) 181
PspGI CCWGG 1 cut(s) 181
PspN4I GGNNCC 3 cut(s) 187, 202, 490
PspPI GGNCC 3 cut(s) 320, 998, 1261
PstI CTGCAG 1 cut(s) 736
PsuI RGATCY 2 cut(s) 679, 878
RsaI GTAC 1 cut(s) 263
RsaNI GTAC 1 cut(s) 262
RseI CAYNNNNRTG 3 cut(s) 209, 429, 794
SapI GCTCTTC 1 cut(s) 747
SaqAI TTAA 4 cut(s) 611, 641, 761, 893
SatI GCNGC 8 cut(s) 165, 196, 199, 278, 1227, 1358, 1433, 1448
Sau3AI GATC 4 cut(s) 88, 679, 847, 878
Sau96I GGNCC 3 cut(s) 320, 998, 1261
SchI GAGTC 1 cut(s) 87
ScrFI CCNGG 1 cut(s) 183
SduI GDGCHC 1 cut(s) 1220
SfaNI GCATC 2 cut(s) 633, 932
SfcI CTRYAG 1 cut(s) 732
SfoI GGCGCC 1 cut(s) 202
SinI GGWCC 1 cut(s) 1261
SmiMI CAYNNNNRTG 3 cut(s) 209, 429, 794
SmlI CTYRAG 3 cut(s) 142, 628, 708
SmoI CTYRAG 3 cut(s) 142, 628, 708
Sse9I AATT 5 cut(s) 315, 407, 762, 1165, 1241
SsiI CCGC 9 cut(s) 15, 23, 123, 195, 198, 305, 888, 921, 1103
SspDI GGCGCC 1 cut(s) 200
SspMI CTAG 5 cut(s) 683, 722, 1256, 1331, 1368
StyD4I CCNGG 1 cut(s) 181
TaaI ACNGT 3 cut(s) 1029, 1222, 1408
TaiI ACGT 3 cut(s) 540, 620, 1327
TaqI TCGA 4 cut(s) 91, 1092, 1377, 1388
TaqII GACCGA 1 cut(s) 1278
TasI AATT 5 cut(s) 315, 407, 762, 1165, 1241
TauI GCSGC 2 cut(s) 198, 201
TfiI GAWTC 5 cut(s) 172, 981, 1021, 1181, 1379
Tru1I TTAA 4 cut(s) 611, 641, 761, 893
Tru9I TTAA 4 cut(s) 611, 641, 761, 893
TscAI CASTG 4 cut(s) 43, 1023, 1050, 1225
TseFI GTSAC 3 cut(s) 38, 561, 1003
TseI GCWGC 6 cut(s) 164, 277, 1226, 1357, 1432, 1447
Tsp45I GTSAC 3 cut(s) 38, 561, 1003
TspDTI ATGAA 6 cut(s) 1173, 1178, 1220, 1253, 1319, 1406
TspGWI ACGGA 4 cut(s) 178, 417, 549, 798
TspRI CASTG 4 cut(s) 43, 1023, 1050, 1225
VpaK11BI GGWCC 1 cut(s) 1261
XagI CCTNNNNNAGG 1 cut(s) 389
XapI RAATTY 1 cut(s) 1165
XbaI TCTAGA 1 cut(s) 1255
XceI RCATGY 2 cut(s) 1254, 1295
XmnI GAANNNNTTC 1 cut(s) 555
XspI CTAG 5 cut(s) 683, 722, 1256, 1331, 1368
Zsp2I ATGCAT 2 cut(s) 243, 705
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.