Rw7G009620

Protein ROOT INITIATION DEFECTIVE 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0012617
Physical Location & Seq
Forward (+)
8970847 .. 8972734
1888 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G009620.1

Sequence Viewer

Length: 603 bp
ATGGGAACAGGGATAACAGTATGGGACATAGACACAGGGGATCGTCTCCTTCACATACCCTCCTGTGCTTCTCCTCCTCGTGCACTTCTGTGCCTGAGGAACCAATTCGTTGTTGCTTCTCAGATTCACAGGCCTGGCTCTGTTGGCGGTGGGGCCATCTCCATGTGGGCCTTAAACAAGCCTCAGCAGCCTCTTTGGAGCTACCCGTTGGAGGCCATTGTATCACTTGCATGCACAAAGGATGGTGTATATCTTGCCGGTGGTGCTCCATCCGGAAATGCTTATCTTTGGGAGGTCAATAGTGGAAAATTGCTCAAAACTTGGCGTGCACATTGTAATTCTTTGAACTGCATGCTGTTTTTTGATGATGGTTCTCTTCTCATTTCTGGCGGAGACGATGGCATGATCTGTGTGTGGTCTTTGATTAGTTTGCTAGATGTGGAAGATTACCAAAGCTTTCCCTCGTTACTAAATTATTCAATGGATCACAAATCCTCTATAACTGGCCTGTCAACTACATCAGGGAGCTCAAGATTGGTCCTAGTATCAAGCTCACTTGATGGCTCATGCAAGGTTACCAAACTCATCTTAGCTTGTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0007275 GO:0007368 GO:0007389 GO:0008150 GO:0009799 GO:0009855 GO:0032501 GO:0032502 GO:0048856 GO:0070121
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

200

Amino Acids

21.36

Weight (kDa)

6.02

Isoelectric Point (pI)

43.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_1st PF25173 6 - 141 5.8e-11 WDR3 first beta-propeller domain
WD40_Gbeta PF25391 47 - 192 8.3e-08 G protein beta WD-40 repeat protein
Beta-prop_WDR3_2nd PF25172 49 - 192 2.5e-07 WDR3 second beta-propeller domain
Beta-prop_EML_2 PF23414 51 - 180 1.3e-07 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 53 - 192 1.6e-12 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 70 - 193 9.3e-09 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 71 - 192 2.2e-11 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 71 - 141 7.7e-08 THOC3 beta-propeller domain
Beta-prop_EML PF23409 72 - 140 3.8e-07 Echinoderm microtubule-associated protein first beta-propeller
WD40_Prp19 PF24814 73 - 194 5.7e-08 Prp19 WD40 domain
WD40 PF00400 102 - 140 1.1e-08 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 272
AciI CCGC 2 cut(s) 147, 390
AclWI GGATC 2 cut(s) 48, 492
AfiI CCNNNNNNNGG 1 cut(s) 211
AgsI TTSAA 2 cut(s) 346, 480
AjnI CCWGG 1 cut(s) 133
AleI CACNNNNGTG 1 cut(s) 88
AluBI AGCT 5 cut(s) 201, 456, 528, 552, 593
AluI AGCT 5 cut(s) 201, 456, 528, 552, 593
Alw21I GWGCWC 4 cut(s) 85, 268, 331, 530
Alw26I GTCTC 2 cut(s) 50, 387
Alw44I GTGCAC 2 cut(s) 81, 327
AlwI GGATC 2 cut(s) 48, 492
Aor13HI TCCGGA 1 cut(s) 272
AoxI GGCC 5 cut(s) 131, 153, 168, 213, 505
ApaLI GTGCAC 2 cut(s) 81, 327
ApeKI GCWGC 1 cut(s) 187
Asp700I GAANNNNTTC 1 cut(s) 104
AspS9I GGNCC 3 cut(s) 153, 168, 538
AvaII GGWCC 1 cut(s) 538
AxyI CCTNAGG 1 cut(s) 95
BaeGI GKGCMC 2 cut(s) 85, 331
BanII GRGCYC 1 cut(s) 530
BauI CACGAG 1 cut(s) 78
Bbv12I GWGCWC 4 cut(s) 85, 268, 331, 530
BbvCI CCTCAGC 1 cut(s) 183
BbvI GCAGC 1 cut(s) 199
BccI CCATC 6 cut(s) 164, 236, 277, 362, 392, 554
BciT130I CCWGG 1 cut(s) 135
BcoDI GTCTC 2 cut(s) 50, 387
BfaI CTAG 2 cut(s) 434, 542
BisI GCNGC 1 cut(s) 188
BlsI GCNGC 1 cut(s) 189
Bme1390I CCNGG 1 cut(s) 135
Bme18I GGWCC 1 cut(s) 538
BmgT120I GGNCC 3 cut(s) 153, 168, 538
BmiI GGNNCC 2 cut(s) 101, 154
BmrFI CCNGG 1 cut(s) 135
Bpu10I CCTNAGC 1 cut(s) 183
BpuEI CTTGAG 1 cut(s) 514
BsaWI WCCGGW 1 cut(s) 272
BsaXI ACNNNNNCTCC 2 cut(s) 44, 74
Bsc4I CCNNNNNNNGG 1 cut(s) 211
Bse118I RCCGGY 1 cut(s) 257
Bse1I ACTGG 1 cut(s) 508
Bse21I CCTNAGG 1 cut(s) 95
BseAI TCCGGA 1 cut(s) 272
BseBI CCWGG 1 cut(s) 135
BseGI GGATG 2 cut(s) 247, 269
BseLI CCNNNNNNNGG 1 cut(s) 211
BseMII CTCAG 3 cut(s) 86, 134, 197
BseNI ACTGG 1 cut(s) 508
BseRI GAGGAG 2 cut(s) 63, 66
BseSI GKGCMC 2 cut(s) 85, 331
BseXI GCAGC 1 cut(s) 199
BshFI GGCC 5 cut(s) 133, 155, 170, 215, 507
BsiHKAI GWGCWC 4 cut(s) 85, 268, 331, 530
BsiSI CCGG 2 cut(s) 258, 273
BslFI GGGAC 1 cut(s) 38
BslI CCNNNNNNNGG 1 cut(s) 211
BsmAI GTCTC 2 cut(s) 50, 387
BsmBI CGTCTC 2 cut(s) 50, 387
BsmFI GGGAC 1 cut(s) 38
BsnI GGCC 5 cut(s) 133, 155, 170, 215, 507
Bsp1286I GDGCHC 4 cut(s) 85, 268, 331, 530
Bsp13I TCCGGA 1 cut(s) 272
Bsp143I GATC 3 cut(s) 40, 405, 484
BspACI CCGC 2 cut(s) 147, 390
BspANI GGCC 5 cut(s) 133, 155, 170, 215, 507
BspCNI CTCAG 3 cut(s) 87, 133, 196
BspEI TCCGGA 1 cut(s) 272
BspLI GGNNCC 2 cut(s) 101, 154
BspPI GGATC 2 cut(s) 48, 492
BsrFI RCCGGY 1 cut(s) 257
BsrI ACTGG 1 cut(s) 508
BssAI RCCGGY 1 cut(s) 257
BssMI GATC 3 cut(s) 40, 405, 484
BssSI CACGAG 1 cut(s) 78
Bst2BI CACGAG 1 cut(s) 78
Bst2UI CCWGG 1 cut(s) 135
Bst4CI ACNGT 1 cut(s) 19
Bst6I CTCTTC 1 cut(s) 381
BstC8I GCNNGC 3 cut(s) 232, 327, 353
BstDEI CTNAG 4 cut(s) 95, 120, 183, 589
BstEII GGTNACC 1 cut(s) 574
BstF5I GGATG 2 cut(s) 247, 269
BstKTI GATC 3 cut(s) 43, 408, 487
BstMAI GTCTC 2 cut(s) 50, 387
BstMBI GATC 3 cut(s) 40, 405, 484
BstMWI GCNNNNNNNGC 3 cut(s) 144, 187, 263
BstNI CCWGG 1 cut(s) 135
BstNSI RCATGY 2 cut(s) 234, 355
BstPI GGTNACC 1 cut(s) 574
BstSCI CCNGG 1 cut(s) 133
BstSLI GKGCMC 2 cut(s) 85, 331
BstV1I GCAGC 1 cut(s) 199
Bsu36I CCTNAGG 1 cut(s) 95
BsuRI GGCC 5 cut(s) 133, 155, 170, 215, 507
BtsCI GGATG 2 cut(s) 247, 269
Cac8I GCNNGC 3 cut(s) 232, 327, 353
Cfr10I RCCGGY 1 cut(s) 257
Cfr13I GGNCC 3 cut(s) 153, 168, 538
CviAII CATG 5 cut(s) 163, 231, 352, 403, 567
DdeI CTNAG 4 cut(s) 95, 120, 183, 589
DpnI GATC 3 cut(s) 42, 407, 486
DpnII GATC 3 cut(s) 40, 405, 484
Eam1104I CTCTTC 1 cut(s) 381
EarI CTCTTC 1 cut(s) 381
EciI GGCGGA 1 cut(s) 405
Ecl136II GAGCTC 1 cut(s) 528
Eco147I AGGCCT 1 cut(s) 133
Eco24I GRGCYC 1 cut(s) 530
Eco47I GGWCC 1 cut(s) 538
Eco53kI GAGCTC 1 cut(s) 528
Eco81I CCTNAGG 1 cut(s) 95
Eco91I GGTNACC 1 cut(s) 574
EcoICRI GAGCTC 1 cut(s) 528
EcoO65I GGTNACC 1 cut(s) 574
EcoRII CCWGG 1 cut(s) 133
EcoT38I GRGCYC 1 cut(s) 530
Esp3I CGTCTC 2 cut(s) 50, 387
FaeI CATG 5 cut(s) 166, 234, 355, 406, 570
FaqI GGGAC 1 cut(s) 38
FatI CATG 5 cut(s) 162, 230, 351, 402, 566
Fnu4HI GCNGC 1 cut(s) 188
FokI GGATG 2 cut(s) 254, 256
FriOI GRGCYC 1 cut(s) 530
Fsp4HI GCNGC 1 cut(s) 188
FspBI CTAG 2 cut(s) 434, 542
GluI GCNGC 1 cut(s) 188
HaeIII GGCC 5 cut(s) 133, 155, 170, 215, 507
HapII CCGG 2 cut(s) 258, 273
Hin1II CATG 5 cut(s) 166, 234, 355, 406, 570
HincII GTYRAC 1 cut(s) 513
HindII GTYRAC 1 cut(s) 513
HindIII AAGCTT 1 cut(s) 454
HinfI GANTC 1 cut(s) 124
HpaII CCGG 2 cut(s) 258, 273
Hpy166II GTNNAC 3 cut(s) 83, 329, 513
Hpy188I TCNGA 1 cut(s) 123
Hpy188III TCNNGA 2 cut(s) 273, 531
Hpy8I GTNNAC 3 cut(s) 83, 329, 513
HpyAV CCTTC 1 cut(s) 59
HpyCH4III ACNGT 1 cut(s) 19
HpyCH4V TGCA 6 cut(s) 83, 230, 234, 329, 351, 570
HpyF10VI GCNNNNNNNGC 3 cut(s) 144, 187, 263
HpyF3I CTNAG 4 cut(s) 95, 120, 183, 589
Hsp92II CATG 5 cut(s) 166, 234, 355, 406, 570
Kpn2I TCCGGA 1 cut(s) 272
Kzo9I GATC 3 cut(s) 40, 405, 484
LmnI GCTCC 3 cut(s) 198, 271, 525
Lsp1109I GCAGC 1 cut(s) 199
MaeI CTAG 2 cut(s) 434, 542
MaeIII GTNAC 2 cut(s) 465, 574
MalI GATC 3 cut(s) 42, 407, 486
MboI GATC 3 cut(s) 40, 405, 484
MboII GAAGA 2 cut(s) 368, 455
MhlI GDGCHC 4 cut(s) 85, 268, 331, 530
MluCI AATT 4 cut(s) 104, 308, 337, 472
MmeI TCCRAC 1 cut(s) 189
MroI TCCGGA 1 cut(s) 272
MroXI GAANNNNTTC 1 cut(s) 104
MseI TTAA 1 cut(s) 173
MslI CAYNNNNRTG 3 cut(s) 88, 161, 229
MspI CCGG 2 cut(s) 258, 273
MspR9I CCNGG 1 cut(s) 135
MvaI CCWGG 1 cut(s) 135
MwoI GCNNNNNNNGC 3 cut(s) 144, 187, 263
NdeII GATC 3 cut(s) 40, 405, 484
NlaIII CATG 5 cut(s) 166, 234, 355, 406, 570
NlaIV GGNNCC 2 cut(s) 101, 154
NspI RCATGY 2 cut(s) 234, 355
OliI CACNNNNGTG 1 cut(s) 88
PaeI GCATGC 2 cut(s) 234, 355
PceI AGGCCT 1 cut(s) 133
PdmI GAANNNNTTC 1 cut(s) 104
PfeI GAWTC 1 cut(s) 124
PkrI GCNGC 1 cut(s) 189
Psp124BI GAGCTC 1 cut(s) 530
Psp6I CCWGG 1 cut(s) 133
PspEI GGTNACC 1 cut(s) 574
PspGI CCWGG 1 cut(s) 133
PspN4I GGNNCC 2 cut(s) 101, 154
PspPI GGNCC 3 cut(s) 153, 168, 538
RseI CAYNNNNRTG 3 cut(s) 88, 161, 229
SacI GAGCTC 1 cut(s) 530
SaqAI TTAA 1 cut(s) 173
SatI GCNGC 1 cut(s) 188
Sau3AI GATC 3 cut(s) 40, 405, 484
Sau96I GGNCC 3 cut(s) 153, 168, 538
ScrFI CCNGG 1 cut(s) 135
SduI GDGCHC 4 cut(s) 85, 268, 331, 530
SetI ASST 7 cut(s) 203, 297, 458, 530, 554, 576, 595
SinI GGWCC 1 cut(s) 538
SmiMI CAYNNNNRTG 3 cut(s) 88, 161, 229
SmlI CTYRAG 1 cut(s) 529
SmoI CTYRAG 1 cut(s) 529
SphI GCATGC 2 cut(s) 234, 355
Sse9I AATT 4 cut(s) 104, 308, 337, 472
SseBI AGGCCT 1 cut(s) 133
SsiI CCGC 2 cut(s) 147, 390
SspMI CTAG 2 cut(s) 434, 542
SstI GAGCTC 1 cut(s) 530
StuI AGGCCT 1 cut(s) 133
StyD4I CCNGG 1 cut(s) 133
TaaI ACNGT 1 cut(s) 19
TasI AATT 4 cut(s) 104, 308, 337, 472
TfiI GAWTC 1 cut(s) 124
Tru1I TTAA 1 cut(s) 173
Tru9I TTAA 1 cut(s) 173
TseI GCWGC 1 cut(s) 187
VneI GTGCAC 2 cut(s) 81, 327
VpaK11BI GGWCC 1 cut(s) 538
XceI RCATGY 2 cut(s) 234, 355
XmnI GAANNNNTTC 1 cut(s) 104
XspI CTAG 2 cut(s) 434, 542
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.