RchiOBHm_Chr7g0190461

Protein ROOT INITIATION DEFECTIVE 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0012617
Physical Location & Seq
Forward (+)
9520453 .. 9523740
3288 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ17011

Sequence Viewer

Length: 1245 bp
ATGTATATAACTCTCCACAGTTCACTCTCTAGTCTTTACCTCTTTACAGAGAGAGAGAGAGAGATGGGCGGTAATGGAGGCGAGGCAGTGGTTGTGTGCAGTGACAAGAGCATGGGAACAGGGATAACAGTATGGGACATAGGCACAGGGGATCGTCTCCTTCACATACCCTCCTGTGCTTCTCCTCCTCGTGCACTTCTGTGCCTGAGGAACCAGTTCATTGTTGCATCTCAGATTCACAGGCCTGGCTCTGTTGGCGGTGGGGCCATCTCCATGTGGGCCTTAAACAAGCAGCCTCTTTGGAGCTACCCGTTGGAGGCCATTGTATCACTTGCATGCACAAAGGATGGTGTATATCTTGCCGGTGGTGCTCCATCCGGAAATGCTTATCTTTGGGAGGTCAATAGTGGAAAATTGCTCAAAACTTGGCGTGCACATTGTAATTCTTTGAACTGCATGCTGTTTTTTGATGATGGTTCTCTTCTCATTTCTGGCGGAGACGATGGCATGATCTGTGTTTGGTCTTTGATTAGTTTGCTAGATGTGGAAGATTACCAAAGCTTTCCCTCGTTACTAAGTTATTCAATGGATCACAAATCCTCTATAACTGGCCTGTCAACGACATCAGGGAGCTCAAGATTGGTCCTAGTATCAAGCTCACTTGATGGCTCATGCAAGGTTTGGGATTTAGGCTTGGGAAAGCTTCTGCAAACTCAAGTTTATCCAATATCGATAACTGCACTTATTCTTAACCCAACAGAGCAGCTCTTTTTCTCTGGTAGTGTAGATGGAAGAATTTTCGTGAACCAACTCGAAATTGGACTGGTGGATGAGGACCCTTTGTTCGCTGTTGAAGATCAAGCAGTTGTGCTAAAAGGACACAATGTATCTATTACTGCATTGACCTTCTGCCAATCGGGTCTGATATCTGCATCTGAGGATTGCACCATCTGCATCTGGGATGTCACCACTTGGGCAATCATTCGTAGATTCGACCATCAGAAAGGGGCTGTAACTAATCTGGCAGTGATCCCACGGTCCTCACTGCTTCCTGGGTCAAACCAACGGAGAGGGTCTGGTGTTTCCTTACTCGAGAAGTATCCTCAGCCTGCCAACTCATCCAGGGGAGACATGAGTATCGCTCTTGCTTCGCCATGCCAATTCCTGAAAGAGAACCAGACCTCGATCAATTTTCAAACCACTGACCAACATATATATGACATGGAGGTCTCTCTCAAATCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0007275 GO:0007368 GO:0007389 GO:0008150 GO:0009799 GO:0009855 GO:0032501 GO:0032502 GO:0048856 GO:0070121
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

414

Amino Acids

44.82

Weight (kDa)

5.27

Isoelectric Point (pI)

38.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_EML_2 PF23414 89 - 205 2.3e-06 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_2nd PF25172 102 - 348 2e-12 WDR3 second beta-propeller domain
Beta-prop_WDR3_1st PF25173 104 - 268 1.1e-18 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 106 - 230 4.9e-10 CDC20/Fizzy WD40 domain
Beta-prop_EML PF23409 106 - 175 1.6e-06 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_THOC3 PF25174 107 - 176 4.2e-07 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 108 - 240 2.2e-15 WDR5 beta-propeller domain
WD40_Gbeta PF25391 108 - 229 8.1e-08 G protein beta WD-40 repeat protein
WD40_Prp19 PF24814 108 - 238 1.9e-09 Prp19 WD40 domain
WD40 PF00400 137 - 175 3.3e-08 WD domain, G-beta repeat
Beta-prop_TEP1_2nd PF25047 142 - 238 1.7e-06 TEP-1 second beta-propeller
EIF3I PF24805 148 - 345 8.9e-07 EIF3I
WD40_WDHD1_1st PF24817 197 - 343 3.2e-08 WDHD1 first WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 197 - 350 4.6e-10 WDR36/Utp21 second beta-propeller domain
Beta-prop_THOC3 PF25174 216 - 350 1.5e-10 THOC3 beta-propeller domain
WD40_Prp19 PF24814 245 - 349 3.7e-06 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 250 - 349 3.1e-06 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 286 - 350 1.1e-09 WDR3 first beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1226
AccIII TCCGGA 1 cut(s) 377
AciI CCGC 3 cut(s) 69, 258, 495
AclWI GGATC 3 cut(s) 159, 597, 1024
AcsI RAATTY 1 cut(s) 795
AfiI CCNNNNNNNGG 1 cut(s) 316
AgsI TTSAA 4 cut(s) 451, 585, 854, 1196
AjnI CCWGG 3 cut(s) 244, 1051, 1121
AleI CACNNNNGTG 1 cut(s) 199
AloI GAACNNNNNNTCC 2 cut(s) 827, 859
AluBI AGCT 6 cut(s) 306, 561, 633, 657, 703, 766
AluI AGCT 6 cut(s) 306, 561, 633, 657, 703, 766
Alw21I GWGCWC 4 cut(s) 196, 373, 436, 635
Alw26I GTCTC 4 cut(s) 161, 492, 1122, 1234
Alw44I GTGCAC 2 cut(s) 192, 432
AlwI GGATC 3 cut(s) 159, 597, 1024
Ama87I CYCGRG 1 cut(s) 1091
Aor13HI TCCGGA 1 cut(s) 377
AoxI GGCC 5 cut(s) 242, 264, 279, 318, 610
ApaLI GTGCAC 2 cut(s) 192, 432
ApeKI GCWGC 2 cut(s) 292, 763
ApoI RAATTY 1 cut(s) 795
ArsI GACNNNNNNTTYG 2 cut(s) 827, 859
Asp700I GAANNNNTTC 1 cut(s) 215
AspS9I GGNCC 5 cut(s) 264, 279, 643, 835, 1038
AsuHPI GGTGA 1 cut(s) 958
AvaI CYCGRG 1 cut(s) 1091
AvaII GGWCC 3 cut(s) 643, 835, 1038
AxyI CCTNAGG 1 cut(s) 206
BaeGI GKGCMC 2 cut(s) 196, 436
BaeI ACNNNNGTAYC 2 cut(s) 1120, 1153
BanII GRGCYC 1 cut(s) 635
BauI CACGAG 1 cut(s) 189
Bbv12I GWGCWC 4 cut(s) 196, 373, 436, 635
BbvCI CCTCAGC 1 cut(s) 1104
BbvI GCAGC 2 cut(s) 304, 775
BciT130I CCWGG 3 cut(s) 246, 1053, 1123
BciVI GTATCC 1 cut(s) 1110
BcoDI GTCTC 4 cut(s) 161, 492, 1122, 1234
BfaI CTAG 4 cut(s) 30, 539, 647, 1243
BfuI GTATCC 1 cut(s) 1110
BisI GCNGC 2 cut(s) 293, 764
BlsI GCNGC 2 cut(s) 294, 765
Bme1390I CCNGG 3 cut(s) 246, 1053, 1123
Bme18I GGWCC 3 cut(s) 643, 835, 1038
BmeT110I CYCGRG 1 cut(s) 1091
BmgT120I GGNCC 5 cut(s) 264, 279, 643, 835, 1038
BmiI GGNNCC 3 cut(s) 212, 265, 837
BmrFI CCNGG 3 cut(s) 246, 1053, 1123
BmsI GCATC 3 cut(s) 236, 941, 963
BplI GAGNNNNNCTC 2 cut(s) 1126, 1158
Bpu10I CCTNAGC 1 cut(s) 1104
BpuEI CTTGAG 2 cut(s) 619, 699
Bsa29I ATCGAT 1 cut(s) 731
BsaI GGTCTC 1 cut(s) 1234
BsaJI CCNNGG 3 cut(s) 1034, 1052, 1122
BsaWI WCCGGW 1 cut(s) 377
BsaXI ACNNNNNCTCC 2 cut(s) 155, 185
Bsc4I CCNNNNNNNGG 1 cut(s) 316
Bse118I RCCGGY 1 cut(s) 362
Bse1I ACTGG 3 cut(s) 214, 613, 828
Bse21I CCTNAGG 1 cut(s) 206
BseAI TCCGGA 1 cut(s) 377
BseBI CCWGG 3 cut(s) 246, 1053, 1123
BseCI ATCGAT 1 cut(s) 731
BseDI CCNNGG 3 cut(s) 1034, 1052, 1122
BseGI GGATG 5 cut(s) 352, 374, 835, 967, 1118
BseLI CCNNNNNNNGG 1 cut(s) 316
BseMII CTCAG 4 cut(s) 197, 245, 927, 1118
BseNI ACTGG 3 cut(s) 214, 613, 828
BseRI GAGGAG 2 cut(s) 174, 177
BseSI GKGCMC 2 cut(s) 196, 436
BseXI GCAGC 2 cut(s) 304, 775
BsgI GTGCAG 2 cut(s) 118, 723
BshFI GGCC 5 cut(s) 244, 266, 281, 320, 612
BshVI ATCGAT 1 cut(s) 731
BsiHKAI GWGCWC 4 cut(s) 196, 373, 436, 635
BsiHKCI CYCGRG 1 cut(s) 1091
BsiSI CCGG 2 cut(s) 363, 378
BslFI GGGAC 1 cut(s) 149
BslI CCNNNNNNNGG 1 cut(s) 316
BsmAI GTCTC 4 cut(s) 161, 492, 1122, 1234
BsmBI CGTCTC 2 cut(s) 161, 492
BsmFI GGGAC 1 cut(s) 149
BsnI GGCC 5 cut(s) 244, 266, 281, 320, 612
Bso31I GGTCTC 1 cut(s) 1234
BsoBI CYCGRG 1 cut(s) 1091
Bsp1286I GDGCHC 4 cut(s) 196, 373, 436, 635
Bsp13I TCCGGA 1 cut(s) 377
Bsp143I GATC 6 cut(s) 151, 510, 589, 856, 1029, 1185
BspACI CCGC 3 cut(s) 69, 258, 495
BspANI GGCC 5 cut(s) 244, 266, 281, 320, 612
BspCNI CTCAG 4 cut(s) 198, 244, 928, 1117
BspDI ATCGAT 1 cut(s) 731
BspEI TCCGGA 1 cut(s) 377
BspLI GGNNCC 3 cut(s) 212, 265, 837
BspPI GGATC 3 cut(s) 159, 597, 1024
BspTNI GGTCTC 1 cut(s) 1234
BsrFI RCCGGY 1 cut(s) 362
BsrI ACTGG 3 cut(s) 214, 613, 828
BssAI RCCGGY 1 cut(s) 362
BssECI CCNNGG 3 cut(s) 1034, 1052, 1122
BssMI GATC 6 cut(s) 151, 510, 589, 856, 1029, 1185
BssSI CACGAG 1 cut(s) 189
Bst2BI CACGAG 1 cut(s) 189
Bst2UI CCWGG 3 cut(s) 246, 1053, 1123
Bst4CI ACNGT 3 cut(s) 20, 130, 1038
Bst6I CTCTTC 1 cut(s) 486
BstAPI GCANNNNNTGC 1 cut(s) 951
BstC8I GCNNGC 4 cut(s) 337, 432, 458, 1110
BstDEI CTNAG 5 cut(s) 206, 231, 575, 936, 1104
BstDSI CCRYGG 1 cut(s) 1034
BstF5I GGATG 5 cut(s) 352, 374, 835, 967, 1118
BstKTI GATC 6 cut(s) 154, 513, 592, 859, 1032, 1188
BstMAI GTCTC 4 cut(s) 161, 492, 1122, 1234
BstMBI GATC 6 cut(s) 151, 510, 589, 856, 1029, 1185
BstMWI GCNNNNNNNGC 3 cut(s) 255, 368, 951
BstNI CCWGG 3 cut(s) 246, 1053, 1123
BstNSI RCATGY 2 cut(s) 339, 460
BstSCI CCNGG 3 cut(s) 244, 1051, 1121
BstSLI GKGCMC 2 cut(s) 196, 436
BstV1I GCAGC 2 cut(s) 304, 775
Bsu15I ATCGAT 1 cut(s) 731
Bsu36I CCTNAGG 1 cut(s) 206
BsuI GTATCC 1 cut(s) 1110
BsuRI GGCC 5 cut(s) 244, 266, 281, 320, 612
BsuTUI ATCGAT 1 cut(s) 731
BtgI CCRYGG 1 cut(s) 1034
BtsCI GGATG 5 cut(s) 352, 374, 835, 967, 1118
BtsI GCAGTG 4 cut(s) 93, 106, 1032, 1043
BtsIMutI CAGTG 5 cut(s) 93, 106, 1032, 1043, 1200
Cac8I GCNNGC 4 cut(s) 337, 432, 458, 1110
Cfr10I RCCGGY 1 cut(s) 362
Cfr13I GGNCC 5 cut(s) 264, 279, 643, 835, 1038
ClaI ATCGAT 1 cut(s) 731
CviAII CATG 9 cut(s) 112, 274, 336, 457, 508, 672, 1132, 1155, 1222
DdeI CTNAG 5 cut(s) 206, 231, 575, 936, 1104
DpnI GATC 6 cut(s) 153, 512, 591, 858, 1031, 1187
DpnII GATC 6 cut(s) 151, 510, 589, 856, 1029, 1185
DrdI GACNNNNNNGTC 1 cut(s) 1226
DseDI GACNNNNNNGTC 1 cut(s) 1226
Eam1104I CTCTTC 1 cut(s) 486
EarI CTCTTC 1 cut(s) 486
EciI GGCGGA 1 cut(s) 510
Ecl136II GAGCTC 1 cut(s) 633
Eco147I AGGCCT 1 cut(s) 244
Eco24I GRGCYC 1 cut(s) 635
Eco31I GGTCTC 1 cut(s) 1234
Eco32I GATATC 1 cut(s) 927
Eco47I GGWCC 3 cut(s) 643, 835, 1038
Eco53kI GAGCTC 1 cut(s) 633
Eco81I CCTNAGG 1 cut(s) 206
Eco88I CYCGRG 1 cut(s) 1091
EcoICRI GAGCTC 1 cut(s) 633
EcoO109I RGGNCCY 1 cut(s) 835
EcoRII CCWGG 3 cut(s) 244, 1051, 1121
EcoRV GATATC 1 cut(s) 927
EcoT38I GRGCYC 1 cut(s) 635
Esp3I CGTCTC 2 cut(s) 161, 492
FaeI CATG 9 cut(s) 115, 277, 339, 460, 511, 675, 1135, 1158, 1225
FaqI GGGAC 1 cut(s) 149
FatI CATG 9 cut(s) 111, 273, 335, 456, 507, 671, 1131, 1154, 1221
Fnu4HI GCNGC 2 cut(s) 293, 764
FokI GGATG 5 cut(s) 359, 361, 842, 974, 1105
FriOI GRGCYC 1 cut(s) 635
Fsp4HI GCNGC 2 cut(s) 293, 764
FspBI CTAG 4 cut(s) 30, 539, 647, 1243
GluI GCNGC 2 cut(s) 293, 764
HaeIII GGCC 5 cut(s) 244, 266, 281, 320, 612
HapII CCGG 2 cut(s) 363, 378
Hin1II CATG 9 cut(s) 115, 277, 339, 460, 511, 675, 1135, 1158, 1225
HincII GTYRAC 1 cut(s) 618
HindII GTYRAC 1 cut(s) 618
HindIII AAGCTT 2 cut(s) 559, 701
HinfI GANTC 2 cut(s) 235, 990
HpaII CCGG 2 cut(s) 363, 378
HphI GGTGA 1 cut(s) 958
Hpy166II GTNNAC 5 cut(s) 23, 194, 434, 618, 805
Hpy188I TCNGA 4 cut(s) 234, 924, 937, 1002
Hpy188III TCNNGA 5 cut(s) 378, 636, 802, 1093, 1165
Hpy8I GTNNAC 5 cut(s) 23, 194, 434, 618, 805
HpyAV CCTTC 2 cut(s) 170, 916
HpyCH4III ACNGT 3 cut(s) 20, 130, 1038
HpyF10VI GCNNNNNNNGC 3 cut(s) 255, 368, 951
HpyF3I CTNAG 5 cut(s) 206, 231, 575, 936, 1104
Hsp92II CATG 9 cut(s) 115, 277, 339, 460, 511, 675, 1135, 1158, 1225
Kpn2I TCCGGA 1 cut(s) 377
Kzo9I GATC 6 cut(s) 151, 510, 589, 856, 1029, 1185
LmnI GCTCC 3 cut(s) 303, 376, 630
Lsp1109I GCAGC 2 cut(s) 304, 775
LweI GCATC 3 cut(s) 236, 941, 963
MaeI CTAG 4 cut(s) 30, 539, 647, 1243
MaeIII GTNAC 4 cut(s) 101, 570, 964, 1012
MalI GATC 6 cut(s) 153, 512, 591, 858, 1031, 1187
MboI GATC 6 cut(s) 151, 510, 589, 856, 1029, 1185
MboII GAAGA 4 cut(s) 473, 560, 804, 866
MhlI GDGCHC 4 cut(s) 196, 373, 436, 635
MluCI AATT 6 cut(s) 413, 442, 795, 816, 1160, 1189
MmeI TCCRAC 1 cut(s) 294
MroI TCCGGA 1 cut(s) 377
MroXI GAANNNNTTC 1 cut(s) 215
MseI TTAA 2 cut(s) 284, 750
MslI CAYNNNNRTG 4 cut(s) 199, 272, 334, 1215
MspI CCGG 2 cut(s) 363, 378
MspR9I CCNGG 3 cut(s) 246, 1053, 1123
MvaI CCWGG 3 cut(s) 246, 1053, 1123
MwoI GCNNNNNNNGC 3 cut(s) 255, 368, 951
NdeII GATC 6 cut(s) 151, 510, 589, 856, 1029, 1185
NlaIII CATG 9 cut(s) 115, 277, 339, 460, 511, 675, 1135, 1158, 1225
NlaIV GGNNCC 3 cut(s) 212, 265, 837
NmuCI GTSAC 2 cut(s) 101, 964
NspI RCATGY 2 cut(s) 339, 460
OliI CACNNNNGTG 1 cut(s) 199
PaeI GCATGC 2 cut(s) 339, 460
PaeR7I CTCGAG 1 cut(s) 1091
PceI AGGCCT 1 cut(s) 244
PdmI GAANNNNTTC 1 cut(s) 215
PfeI GAWTC 2 cut(s) 235, 990
PkrI GCNGC 2 cut(s) 294, 765
PpuMI RGGWCCY 1 cut(s) 835
Psp124BI GAGCTC 1 cut(s) 635
Psp5II RGGWCCY 1 cut(s) 835
Psp6I CCWGG 3 cut(s) 244, 1051, 1121
PspGI CCWGG 3 cut(s) 244, 1051, 1121
PspN4I GGNNCC 3 cut(s) 212, 265, 837
PspPI GGNCC 5 cut(s) 264, 279, 643, 835, 1038
PspPPI RGGWCCY 1 cut(s) 835
RseI CAYNNNNRTG 4 cut(s) 199, 272, 334, 1215
SacI GAGCTC 1 cut(s) 635
SaqAI TTAA 2 cut(s) 284, 750
SatI GCNGC 2 cut(s) 293, 764
Sau3AI GATC 6 cut(s) 151, 510, 589, 856, 1029, 1185
Sau96I GGNCC 5 cut(s) 264, 279, 643, 835, 1038
ScrFI CCNGG 3 cut(s) 246, 1053, 1123
SduI GDGCHC 4 cut(s) 196, 373, 436, 635
SfaNI GCATC 3 cut(s) 236, 941, 963
Sfr274I CTCGAG 1 cut(s) 1091
SinI GGWCC 3 cut(s) 643, 835, 1038
SlaI CTCGAG 1 cut(s) 1091
SmiMI CAYNNNNRTG 4 cut(s) 199, 272, 334, 1215
SmlI CTYRAG 3 cut(s) 634, 714, 1091
SmoI CTYRAG 3 cut(s) 634, 714, 1091
SphI GCATGC 2 cut(s) 339, 460
Sse9I AATT 6 cut(s) 413, 442, 795, 816, 1160, 1189
SseBI AGGCCT 1 cut(s) 244
SsiI CCGC 3 cut(s) 69, 258, 495
SspMI CTAG 4 cut(s) 30, 539, 647, 1243
SstI GAGCTC 1 cut(s) 635
StuI AGGCCT 1 cut(s) 244
StyD4I CCNGG 3 cut(s) 244, 1051, 1121
TaaI ACNGT 3 cut(s) 20, 130, 1038
TaqI TCGA 5 cut(s) 731, 813, 993, 1092, 1184
TasI AATT 6 cut(s) 413, 442, 795, 816, 1160, 1189
TfiI GAWTC 2 cut(s) 235, 990
Tru1I TTAA 2 cut(s) 284, 750
Tru9I TTAA 2 cut(s) 284, 750
TscAI CASTG 5 cut(s) 93, 106, 1032, 1050, 1207
TseFI GTSAC 2 cut(s) 101, 964
TseI GCWGC 2 cut(s) 292, 763
Tsp45I GTSAC 2 cut(s) 101, 964
TspDTI ATGAA 1 cut(s) 208
TspGWI ACGGA 1 cut(s) 1081
TspRI CASTG 5 cut(s) 93, 106, 1032, 1050, 1207
VneI GTGCAC 2 cut(s) 192, 432
VpaK11BI GGWCC 3 cut(s) 643, 835, 1038
XapI RAATTY 1 cut(s) 795
XceI RCATGY 2 cut(s) 339, 460
XcmI CCANNNNNNNNNTGG 1 cut(s) 815
XhoI CTCGAG 1 cut(s) 1091
XmnI GAANNNNTTC 1 cut(s) 215
XspI CTAG 4 cut(s) 30, 539, 647, 1243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.