Rh7BG113400

Protein ROOT INITIATION DEFECTIVE 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0012617
Physical Location & Seq
Forward (+)
8507241 .. 8510629
3389 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG113400.1

Sequence Viewer

Length: 1416 bp
ATGGGTGGTAATGGAGGTGAGGCAGTGGTTGTGTGCAGTGACAAGAGCATGGGAACAGGGATAACAGTATGGGACATAGACACAGGGGATCGTCTCCTTCACATACCCTCCTGTGCTTCTCCTCCTCGTGCACTTTTGTGCCTGAGGAACCAGATCATTGTTGCCTCTCAGATTCACAGGCCTGGCTCTGTTGGCGGTGGGGCCATCTCCATGTGGGCCTTAAACAAGCCTCAGCAGCCTCTTTGGAGCTACCCGTTGGAGGCCATTGTATCACTTGCATGCACAAAGGATGGTGTATATCTTGCCGGTGGTGCTCCATCCGGAAATGCTTATCTTTGGGAGGTCAATAGTGGAAAATTGCTCAAAACTTGGCGTGCACATTGTAATTCTTTGAACTGCATGCTGTTTTTTGATGATGGTTCTCTTCTCATTTCTGGCGGAGACGATGGCATGATCTGTGTGTGGTCTTTGATTAGTTTGCTAGATGTGGAAGATTACCAAAGCTTTCCCTCGTTACTAAATTATTCAATGGATCACAAATCCTCTATAACTGGCCTGTCAACTACATCAGGGAGCTCAAGATTGGTCCTAGTATCAAGCTCACTTGATGGCTCATGCAAGGTTTGGGATTTAGGCTTGGGAAAGCTTCTGCAAACTCAAGTTTATCCAATATCAATAACTGCACTTATTCTTAACCCAACAGAGCAGCTCTTTTTCTCTGGTAGTGTAGACGGAAGAATTTTCGTGAACCAACTCGAAATTGGACTCGTGGATGAGGACCCTTTGTTCGCCGTTGAAGATCAAGCAGTTGTGCTAAAAGGACAAAATGTATCTATTACTGCATTGACCTTCTGCCAATCGGGTCTGATATCTGCATCTGAGGATTGCACCATCTGCATCTGGGATGTCACCACTTGGGCAATCATTCGTAGATTCGACCATCAGAAAGGGGGTGTAACTAATCTGGCAGTGATCCCACGGTCCTCACTGCTTCCGGGGTCAAACCAACGGAGAGGGTCTGGTGTTTCCTTACTCGAGAAGTATCCTCAGCCTGCCAACTCATCCAGAGGAGACATGAGTATCACTCTTGCTTCGCCATGCCAATTCCTGAAAGAGAACCAGACCTCGATCAATTTTCAAACCACTGACCAACATATATATGACATGGAGAAAGAATACACTCCTGCGTCAATGGAAATGAAATTGGAACGAAGTTTAGAGGAGCGGACGTGGGCAACAAGAATGGCAAACCATGTCATGGACATGAATAGGCACCTGCAGTCGCGGTTGTTAGATTTGATGCAGAGCAGCTTTTTATTGTCCAGGTCTACTGAAATGGATTCACCTTCAACCAAAAAGAGAAAGACCCTCATGCTCGAAAGTCCTCCATTACAGGAGGAGGAGAAGCAAGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0007275 GO:0007368 GO:0007389 GO:0008150 GO:0009799 GO:0009855 GO:0032501 GO:0032502 GO:0048856 GO:0070121
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

471

Amino Acids

51.61

Weight (kDa)

5.28

Isoelectric Point (pI)

47.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_EML PF23409 10 - 156 9.6e-07 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR3_1st PF25173 21 - 157 3.7e-10 WDR3 first beta-propeller domain
WD40_Gbeta PF25391 63 - 210 1.7e-08 G protein beta WD-40 repeat protein
Beta-prop_WDR3_2nd PF25172 66 - 329 1.5e-11 WDR3 second beta-propeller domain
Beta-prop_EML_2 PF23414 67 - 186 1.9e-06 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 69 - 249 7.2e-19 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 87 - 211 5.5e-10 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 87 - 157 4.4e-07 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 88 - 243 1.9e-15 WDR5 beta-propeller domain
WD40_Prp19 PF24814 89 - 219 2.9e-09 Prp19 WD40 domain
WD40 PF00400 118 - 156 4e-08 WD domain, G-beta repeat
Beta-prop_TEP1_2nd PF25047 123 - 219 5.6e-06 TEP-1 second beta-propeller
EIF3I PF24805 127 - 327 1.1e-06 EIF3I
WD40_Prp19 PF24814 178 - 323 1e-08 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 178 - 323 6.3e-08 WDHD1 first WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 179 - 328 6.1e-08 WDR36/Utp21 second beta-propeller domain
Beta-prop_THOC3 PF25174 197 - 331 6.7e-09 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 267 - 331 1.9e-07 WDR3 first beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1284
Acc36I ACCTGC 1 cut(s) 1284
AccB1I GGYRCC 1 cut(s) 1272
AccB7I CCANNNNNTGG 1 cut(s) 1258
AccBSI CCGCTC 1 cut(s) 1225
AccI GTMKAC 2 cut(s) 729, 1328
AccII CGCG 1 cut(s) 1285
AccIII TCCGGA 1 cut(s) 320
AciI CCGC 4 cut(s) 195, 438, 1225, 1285
AclWI GGATC 3 cut(s) 96, 540, 967
AcsI RAATTY 1 cut(s) 738
AfiI CCNNNNNNNGG 2 cut(s) 259, 1258
AgsI TTSAA 5 cut(s) 394, 528, 797, 1139, 1350
AjiI CACGTC 1 cut(s) 1230
AjnI CCWGG 2 cut(s) 181, 1322
AleI CACNNNNGTG 1 cut(s) 136
AloI GAACNNNNNNTCC 2 cut(s) 770, 802
AluBI AGCT 7 cut(s) 249, 504, 576, 600, 646, 709, 1311
AluI AGCT 7 cut(s) 249, 504, 576, 600, 646, 709, 1311
Alw21I GWGCWC 4 cut(s) 133, 316, 379, 578
Alw26I GTCTC 3 cut(s) 98, 435, 1065
Alw44I GTGCAC 2 cut(s) 129, 375
AlwI GGATC 3 cut(s) 96, 540, 967
Ama87I CYCGRG 1 cut(s) 1034
Aor13HI TCCGGA 1 cut(s) 320
AoxI GGCC 5 cut(s) 179, 201, 216, 261, 553
ApaLI GTGCAC 2 cut(s) 129, 375
ApeKI GCWGC 3 cut(s) 235, 706, 1308
ApoI RAATTY 1 cut(s) 738
ArsI GACNNNNNNTTYG 2 cut(s) 770, 802
AspS9I GGNCC 5 cut(s) 201, 216, 586, 778, 981
AsuC2I CCSGG 1 cut(s) 996
AsuHPI GGTGA 3 cut(s) 29, 901, 1335
AvaI CYCGRG 1 cut(s) 1034
AvaII GGWCC 3 cut(s) 586, 778, 981
AxyI CCTNAGG 1 cut(s) 143
BaeGI GKGCMC 2 cut(s) 133, 379
BaeI ACNNNNGTAYC 2 cut(s) 1063, 1096
BanI GGYRCC 1 cut(s) 1272
BanII GRGCYC 1 cut(s) 578
BauI CACGAG 2 cut(s) 126, 767
Bbv12I GWGCWC 4 cut(s) 133, 316, 379, 578
BbvCI CCTCAGC 2 cut(s) 231, 1047
BbvI GCAGC 3 cut(s) 247, 718, 1320
BccI CCATC 8 cut(s) 212, 284, 325, 410, 440, 602, 899, 948
BceAI ACGGC 1 cut(s) 776
BciT130I CCWGG 2 cut(s) 183, 1324
BciVI GTATCC 1 cut(s) 1053
BcnI CCSGG 1 cut(s) 996
BcoDI GTCTC 3 cut(s) 98, 435, 1065
BfaI CTAG 2 cut(s) 482, 590
BfmI CTRYAG 1 cut(s) 1277
BfuAI ACCTGC 1 cut(s) 1284
BfuI GTATCC 1 cut(s) 1053
BisI GCNGC 3 cut(s) 236, 707, 1309
BlsI GCNGC 3 cut(s) 237, 708, 1310
Bme1390I CCNGG 3 cut(s) 183, 996, 1324
Bme18I GGWCC 3 cut(s) 586, 778, 981
BmeT110I CYCGRG 1 cut(s) 1034
BmgBI CACGTC 1 cut(s) 1230
BmgT120I GGNCC 5 cut(s) 201, 216, 586, 778, 981
BmiI GGNNCC 4 cut(s) 149, 202, 780, 1274
BmrFI CCNGG 3 cut(s) 183, 996, 1324
BmsI GCATC 3 cut(s) 884, 906, 1290
BplI GAGNNNNNCTC 2 cut(s) 1069, 1101
Bpu10I CCTNAGC 2 cut(s) 231, 1047
BpuEI CTTGAG 2 cut(s) 562, 642
BpuMI CCSGG 1 cut(s) 996
BsaJI CCNNGG 2 cut(s) 977, 995
BsaWI WCCGGW 1 cut(s) 320
BsaXI ACNNNNNCTCC 4 cut(s) 92, 122, 1214, 1244
Bsc4I CCNNNNNNNGG 2 cut(s) 259, 1258
Bse118I RCCGGY 1 cut(s) 305
Bse1I ACTGG 1 cut(s) 556
Bse21I CCTNAGG 1 cut(s) 143
BseAI TCCGGA 1 cut(s) 320
BseBI CCWGG 2 cut(s) 183, 1324
BseDI CCNNGG 2 cut(s) 977, 995
BseGI GGATG 5 cut(s) 295, 317, 778, 910, 1061
BseLI CCNNNNNNNGG 2 cut(s) 259, 1258
BseMII CTCAG 5 cut(s) 134, 182, 245, 870, 1061
BseNI ACTGG 1 cut(s) 556
BseRI GAGGAG 6 cut(s) 111, 114, 1083, 1235, 1412, 1415
BseSI GKGCMC 2 cut(s) 133, 379
BseXI GCAGC 3 cut(s) 247, 718, 1320
BsgI GTGCAG 2 cut(s) 55, 666
Bsh1236I CGCG 1 cut(s) 1285
BshFI GGCC 5 cut(s) 181, 203, 218, 263, 555
BshNI GGYRCC 1 cut(s) 1272
BsiHKAI GWGCWC 4 cut(s) 133, 316, 379, 578
BsiHKCI CYCGRG 1 cut(s) 1034
BsiSI CCGG 3 cut(s) 306, 321, 995
BslFI GGGAC 1 cut(s) 86
BslI CCNNNNNNNGG 2 cut(s) 259, 1258
BsmAI GTCTC 3 cut(s) 98, 435, 1065
BsmBI CGTCTC 2 cut(s) 98, 435
BsmFI GGGAC 1 cut(s) 86
BsnI GGCC 5 cut(s) 181, 203, 218, 263, 555
BsoBI CYCGRG 1 cut(s) 1034
Bsp1286I GDGCHC 4 cut(s) 133, 316, 379, 578
Bsp13I TCCGGA 1 cut(s) 320
Bsp143I GATC 7 cut(s) 88, 153, 453, 532, 799, 972, 1128
BspACI CCGC 4 cut(s) 195, 438, 1225, 1285
BspANI GGCC 5 cut(s) 181, 203, 218, 263, 555
BspCNI CTCAG 5 cut(s) 135, 181, 244, 871, 1060
BspEI TCCGGA 1 cut(s) 320
BspFNI CGCG 1 cut(s) 1285
BspLI GGNNCC 4 cut(s) 149, 202, 780, 1274
BspMAI CTGCAG 1 cut(s) 1281
BspMI ACCTGC 1 cut(s) 1284
BspPI GGATC 3 cut(s) 96, 540, 967
BspT107I GGYRCC 1 cut(s) 1272
BsrBI CCGCTC 1 cut(s) 1225
BsrFI RCCGGY 1 cut(s) 305
BsrI ACTGG 1 cut(s) 556
BssAI RCCGGY 1 cut(s) 305
BssECI CCNNGG 2 cut(s) 977, 995
BssMI GATC 7 cut(s) 88, 153, 453, 532, 799, 972, 1128
BssSI CACGAG 2 cut(s) 126, 767
Bst2BI CACGAG 2 cut(s) 126, 767
Bst2UI CCWGG 2 cut(s) 183, 1324
Bst4CI ACNGT 2 cut(s) 67, 981
Bst6I CTCTTC 1 cut(s) 429
BstAPI GCANNNNNTGC 1 cut(s) 894
BstC8I GCNNGC 4 cut(s) 280, 375, 401, 1053
BstDEI CTNAG 5 cut(s) 143, 168, 231, 879, 1047
BstDSI CCRYGG 1 cut(s) 977
BstF5I GGATG 5 cut(s) 295, 317, 778, 910, 1061
BstFNI CGCG 1 cut(s) 1285
BstKTI GATC 7 cut(s) 91, 156, 456, 535, 802, 975, 1131
BstMAI GTCTC 3 cut(s) 98, 435, 1065
BstMBI GATC 7 cut(s) 88, 153, 453, 532, 799, 972, 1128
BstMWI GCNNNNNNNGC 4 cut(s) 192, 235, 311, 894
BstNI CCWGG 2 cut(s) 183, 1324
BstNSI RCATGY 2 cut(s) 282, 403
BstSCI CCNGG 3 cut(s) 181, 994, 1322
BstSFI CTRYAG 1 cut(s) 1277
BstSLI GKGCMC 2 cut(s) 133, 379
BstUI CGCG 1 cut(s) 1285
BstV1I GCAGC 3 cut(s) 247, 718, 1320
Bsu36I CCTNAGG 1 cut(s) 143
BsuI GTATCC 1 cut(s) 1053
BsuRI GGCC 5 cut(s) 181, 203, 218, 263, 555
BtgI CCRYGG 1 cut(s) 977
BtrI CACGTC 1 cut(s) 1230
BtsCI GGATG 5 cut(s) 295, 317, 778, 910, 1061
BtsI GCAGTG 4 cut(s) 30, 43, 975, 986
BtsIMutI CAGTG 5 cut(s) 30, 43, 975, 986, 1143
BveI ACCTGC 1 cut(s) 1284
Cac8I GCNNGC 4 cut(s) 280, 375, 401, 1053
Cfr10I RCCGGY 1 cut(s) 305
Cfr13I GGNCC 5 cut(s) 201, 216, 586, 778, 981
CseI GACGC 1 cut(s) 1176
DdeI CTNAG 5 cut(s) 143, 168, 231, 879, 1047
DpnI GATC 7 cut(s) 90, 155, 455, 534, 801, 974, 1130
DpnII GATC 7 cut(s) 88, 153, 453, 532, 799, 972, 1128
Eam1104I CTCTTC 1 cut(s) 429
EarI CTCTTC 1 cut(s) 429
EciI GGCGGA 1 cut(s) 453
Ecl136II GAGCTC 1 cut(s) 576
Eco147I AGGCCT 1 cut(s) 181
Eco24I GRGCYC 1 cut(s) 578
Eco32I GATATC 1 cut(s) 870
Eco47I GGWCC 3 cut(s) 586, 778, 981
Eco53kI GAGCTC 1 cut(s) 576
Eco81I CCTNAGG 1 cut(s) 143
Eco88I CYCGRG 1 cut(s) 1034
EcoICRI GAGCTC 1 cut(s) 576
EcoO109I RGGNCCY 1 cut(s) 778
EcoRII CCWGG 2 cut(s) 181, 1322
EcoRV GATATC 1 cut(s) 870
EcoT38I GRGCYC 1 cut(s) 578
Esp3I CGTCTC 2 cut(s) 98, 435
FaqI GGGAC 1 cut(s) 86
FblI GTMKAC 2 cut(s) 729, 1328
Fnu4HI GCNGC 3 cut(s) 236, 707, 1309
FokI GGATG 5 cut(s) 302, 304, 785, 917, 1048
FriOI GRGCYC 1 cut(s) 578
Fsp4HI GCNGC 3 cut(s) 236, 707, 1309
FspBI CTAG 2 cut(s) 482, 590
GluI GCNGC 3 cut(s) 236, 707, 1309
HaeIII GGCC 5 cut(s) 181, 203, 218, 263, 555
HapII CCGG 3 cut(s) 306, 321, 995
HgaI GACGC 1 cut(s) 1176
HincII GTYRAC 1 cut(s) 561
HindII GTYRAC 1 cut(s) 561
HindIII AAGCTT 2 cut(s) 502, 644
HinfI GANTC 4 cut(s) 172, 765, 933, 1340
HpaII CCGG 3 cut(s) 306, 321, 995
HphI GGTGA 3 cut(s) 29, 901, 1335
Hpy166II GTNNAC 6 cut(s) 131, 377, 561, 730, 748, 1329
Hpy188I TCNGA 4 cut(s) 171, 867, 880, 945
Hpy188III TCNNGA 6 cut(s) 321, 579, 745, 1036, 1065, 1108
Hpy8I GTNNAC 6 cut(s) 131, 377, 561, 730, 748, 1329
HpyAV CCTTC 3 cut(s) 107, 859, 1356
HpyCH4III ACNGT 2 cut(s) 67, 981
HpyCH4IV ACGT 1 cut(s) 1229
HpyF10VI GCNNNNNNNGC 4 cut(s) 192, 235, 311, 894
HpyF3I CTNAG 5 cut(s) 143, 168, 231, 879, 1047
HpySE526I ACGT 1 cut(s) 1229
Kpn2I TCCGGA 1 cut(s) 320
Kzo9I GATC 7 cut(s) 88, 153, 453, 532, 799, 972, 1128
LmnI GCTCC 4 cut(s) 246, 319, 573, 1222
Lsp1109I GCAGC 3 cut(s) 247, 718, 1320
LweI GCATC 3 cut(s) 884, 906, 1290
MaeI CTAG 2 cut(s) 482, 590
MaeII ACGT 1 cut(s) 1229
MaeIII GTNAC 4 cut(s) 38, 513, 907, 955
MalI GATC 7 cut(s) 90, 155, 455, 534, 801, 974, 1130
MbiI CCGCTC 1 cut(s) 1225
MboI GATC 7 cut(s) 88, 153, 453, 532, 799, 972, 1128
MboII GAAGA 4 cut(s) 416, 503, 747, 809
MhlI GDGCHC 4 cut(s) 133, 316, 379, 578
MluCI AATT 8 cut(s) 356, 385, 520, 738, 759, 1103, 1132, 1202
MlyI GAGTC 1 cut(s) 759
MmeI TCCRAC 1 cut(s) 237
MroI TCCGGA 1 cut(s) 320
MseI TTAA 2 cut(s) 221, 693
MslI CAYNNNNRTG 5 cut(s) 136, 209, 277, 1158, 1262
MspI CCGG 3 cut(s) 306, 321, 995
MspR9I CCNGG 3 cut(s) 183, 996, 1324
MvaI CCWGG 2 cut(s) 183, 1324
MvnI CGCG 1 cut(s) 1285
MwoI GCNNNNNNNGC 4 cut(s) 192, 235, 311, 894
NciI CCSGG 1 cut(s) 996
NdeII GATC 7 cut(s) 88, 153, 453, 532, 799, 972, 1128
NlaIV GGNNCC 4 cut(s) 149, 202, 780, 1274
NmuCI GTSAC 2 cut(s) 38, 907
NspI RCATGY 2 cut(s) 282, 403
OliI CACNNNNGTG 1 cut(s) 136
PaeI GCATGC 2 cut(s) 282, 403
PaeR7I CTCGAG 1 cut(s) 1034
PaqCI CACCTGC 1 cut(s) 1284
PceI AGGCCT 1 cut(s) 181
PfeI GAWTC 3 cut(s) 172, 933, 1340
PflMI CCANNNNNTGG 1 cut(s) 1258
PkrI GCNGC 3 cut(s) 237, 708, 1310
PleI GAGTC 1 cut(s) 759
PpsI GAGTC 1 cut(s) 759
PpuMI RGGWCCY 1 cut(s) 778
Psp124BI GAGCTC 1 cut(s) 578
Psp5II RGGWCCY 1 cut(s) 778
Psp6I CCWGG 2 cut(s) 181, 1322
PspGI CCWGG 2 cut(s) 181, 1322
PspN4I GGNNCC 4 cut(s) 149, 202, 780, 1274
PspPI GGNCC 5 cut(s) 201, 216, 586, 778, 981
PspPPI RGGWCCY 1 cut(s) 778
PstI CTGCAG 1 cut(s) 1281
RseI CAYNNNNRTG 5 cut(s) 136, 209, 277, 1158, 1262
SacI GAGCTC 1 cut(s) 578
SaqAI TTAA 2 cut(s) 221, 693
SatI GCNGC 3 cut(s) 236, 707, 1309
Sau3AI GATC 7 cut(s) 88, 153, 453, 532, 799, 972, 1128
Sau96I GGNCC 5 cut(s) 201, 216, 586, 778, 981
SchI GAGTC 1 cut(s) 759
ScrFI CCNGG 3 cut(s) 183, 996, 1324
SduI GDGCHC 4 cut(s) 133, 316, 379, 578
SfaNI GCATC 3 cut(s) 884, 906, 1290
SfcI CTRYAG 1 cut(s) 1277
Sfr274I CTCGAG 1 cut(s) 1034
SinI GGWCC 3 cut(s) 586, 778, 981
SlaI CTCGAG 1 cut(s) 1034
SmiMI CAYNNNNRTG 5 cut(s) 136, 209, 277, 1158, 1262
SmlI CTYRAG 3 cut(s) 577, 657, 1034
SmoI CTYRAG 3 cut(s) 577, 657, 1034
SphI GCATGC 2 cut(s) 282, 403
Sse9I AATT 8 cut(s) 356, 385, 520, 738, 759, 1103, 1132, 1202
SseBI AGGCCT 1 cut(s) 181
SsiI CCGC 4 cut(s) 195, 438, 1225, 1285
SspMI CTAG 2 cut(s) 482, 590
SstI GAGCTC 1 cut(s) 578
StuI AGGCCT 1 cut(s) 181
StyD4I CCNGG 3 cut(s) 181, 994, 1322
TaaI ACNGT 2 cut(s) 67, 981
TaiI ACGT 1 cut(s) 1232
TaqI TCGA 5 cut(s) 756, 936, 1035, 1127, 1377
TasI AATT 8 cut(s) 356, 385, 520, 738, 759, 1103, 1132, 1202
TfiI GAWTC 3 cut(s) 172, 933, 1340
Tru1I TTAA 2 cut(s) 221, 693
Tru9I TTAA 2 cut(s) 221, 693
TscAI CASTG 5 cut(s) 30, 43, 975, 993, 1150
TseFI GTSAC 2 cut(s) 38, 907
TseI GCWGC 3 cut(s) 235, 706, 1308
Tsp45I GTSAC 2 cut(s) 38, 907
TspDTI ATGAA 2 cut(s) 1214, 1280
TspGWI ACGGA 2 cut(s) 747, 1024
TspRI CASTG 5 cut(s) 30, 43, 975, 993, 1150
Van91I CCANNNNNTGG 1 cut(s) 1258
VneI GTGCAC 2 cut(s) 129, 375
VpaK11BI GGWCC 3 cut(s) 586, 778, 981
XapI RAATTY 1 cut(s) 738
XceI RCATGY 2 cut(s) 282, 403
XcmI CCANNNNNNNNNTGG 1 cut(s) 758
XhoI CTCGAG 1 cut(s) 1034
XmiI GTMKAC 2 cut(s) 729, 1328
XspI CTAG 2 cut(s) 482, 590
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.