MD06G1165100.v1.1

Plant protein of unknown function (DUF868)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0015623
Physical Location & Seq
Reverse (-)
30536898 .. 30537911
1014 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1165100.v1.1.491

Sequence Viewer

Length: 1014 bp
ATGCAAGACCACATTGGCATTCCTGCTTGCTTCTCCTCAGGTGAGAAGTTAAGTGATGATGCATCAGCTGTGGCTAGGTCAGGCCACAGTGTCTACATGAGAGTGTACAGAACCAAGATAGCCGATCAGAGCCGCTTGATCACGATCACATGGTGCAAAAACCTGCTCCTCCATGGCCTATCGGTGACGATGGAGGGCCCGGATGGAGATCAAACCCAGCAGCACACCCAACACAGCTTCAAAGTTGAGCTCAAGCCATGGTACTTCTGGAGGAAGCAAGGCTCCAAGCGCTTTGTGGTTGATGGGAAGGCAGTGGAGATCTTCTGGGACCTCAAGGCCGCAAAATTCAACGGCGGGACGGAGCCCAAATCAGAGTACTACGTTGCGGTTCTTTGTGACAAGGAAGTTGTTTTGCTTCTTGGTGATCTCAAAAAAGATGCATACAGAAAAACAGGTTTTAGACCTGCCCTCACTGATCCCACTTTGGTTTCTAAAAAAGAGCACATTTTTGGTAAGAAAAAGTTTTCCACAAAAGCTAAGTTTTATGAAAAAGGCAGGCTGCATGAAATTGCAATTGAATGCAAACACAGAGGTGGCGGTGGCGGCGACGGGAGCATTGGGAGTGGAAGTACCCTAAATGGGGTGGAGCCGGAGTTGGAGATTAGGGTTGATGGGCATTTGGTGGTTCATGTGAAGCATCTTCAATGGAAGTTTAGAGGTAATGAGTCCATTCGTATCAACAAGTTGAGAATTGAGGTTTATTGGGATGTCCATGACTGGCTTTTTAGTCCAGGATTAAGGCGTGCATTGTTTATTTTCAAGCCAAGTTTGCCTGCTTCAAGTACATCTCTGTCACCGTTATCGAGAACCTCGTCATCCTCGCCGCCATTTTCTTCATCGGCATCAACTCCATTGTCCTCTCAGACTAGTTCTGCATCAGTAGAAGGGATTAATGCAAGTTCTGCTGGTTCATCCGAGTTTTGCTTATTTCTTTATGCCTGGAAGATTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF868

Protein Analysis

338

Amino Acids

37.5

Weight (kDa)

9.32

Isoelectric Point (pI)

44.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF868 PF05910 28 - 335 5.4e-104 Plant protein of unknown function (DUF868)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015623)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g06390
malus_domestica MD06G1165100.v1.1 MD14G1170600.v1.1
prunus_persica Prupe.5G164700_v2.0.a1
pyrus_communis pycom06g14760 pycom14g14240
rosa_chinensis RchiOBHm_Chr7g0192421
rosa_laevigata RLG00000004369
rosa_multiflora Rmu_sc0003108.1_g000024
rosa_roxburghii Rroxscaffold_3G00263020
rosa_rugosa Rorug07G0001500
rosa_samantha Rh7AG125300 Rh7BG127600 Rh7CG129300 Rh7DG129400
rosa_wichuraiana Rw7G010790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 171, 472
AccI GTMKAC 1 cut(s) 93
AciI CCGC 7 cut(s) 133, 339, 354, 386, 597, 603, 884
AclWI GGATC 1 cut(s) 470
AcsI RAATTY 1 cut(s) 344
AdeI CACNNNGTG 1 cut(s) 153
AfaI GTAC 5 cut(s) 107, 263, 377, 631, 844
AfeI AGCGCT 1 cut(s) 290
AfiI CCNNNNNNNGG 2 cut(s) 639, 640
AgsI TTSAA 7 cut(s) 241, 349, 578, 704, 820, 840, 1010
AhlI ACTAGT 1 cut(s) 926
AjnI CCWGG 2 cut(s) 790, 998
AleI CACNNNNGTG 1 cut(s) 591
AluBI AGCT 4 cut(s) 68, 237, 250, 536
AluI AGCT 4 cut(s) 68, 237, 250, 536
Alw21I GWGCWC 2 cut(s) 252, 504
AlwI GGATC 1 cut(s) 470
Aor51HI AGCGCT 1 cut(s) 290
AoxI GGCC 4 cut(s) 82, 175, 196, 336
ApaI GGGCCC 1 cut(s) 200
ApeKI GCWGC 2 cut(s) 220, 559
ApoI RAATTY 1 cut(s) 344
AseI ATTAAT 1 cut(s) 951
AspLEI GCGC 1 cut(s) 291
AspS9I GGNCC 3 cut(s) 196, 197, 328
AsuC2I CCSGG 1 cut(s) 200
AsuHPI GGTGA 4 cut(s) 53, 196, 434, 846
AvaII GGWCC 1 cut(s) 328
AxyI CCTNAGG 1 cut(s) 37
BaeGI GKGCMC 1 cut(s) 200
BanII GRGCYC 3 cut(s) 200, 252, 366
Bbv12I GWGCWC 2 cut(s) 252, 504
BbvI GCAGC 2 cut(s) 232, 546
BccI CCATC 4 cut(s) 184, 197, 296, 665
BceAI ACGGC 1 cut(s) 367
BciT130I CCWGG 2 cut(s) 792, 1000
BclI TGATCA 1 cut(s) 138
BcnI CCSGG 1 cut(s) 200
BcuI ACTAGT 1 cut(s) 926
BfaI CTAG 2 cut(s) 75, 927
BfoI RGCGCY 1 cut(s) 292
BfuAI ACCTGC 2 cut(s) 171, 472
BglII AGATCT 1 cut(s) 318
BisI GCNGC 6 cut(s) 133, 221, 339, 560, 604, 884
BlsI GCNGC 6 cut(s) 134, 222, 340, 561, 605, 885
BmcAI AGTACT 1 cut(s) 377
Bme1390I CCNGG 3 cut(s) 200, 792, 1000
Bme18I GGWCC 1 cut(s) 328
BmgT120I GGNCC 3 cut(s) 196, 197, 328
BmiI GGNNCC 5 cut(s) 198, 283, 329, 363, 648
BmrFI CCNGG 3 cut(s) 200, 792, 1000
BmsI GCATC 6 cut(s) 49, 71, 427, 706, 911, 944
BpmI CTGGAG 1 cut(s) 289
BpuEI CTTGAG 2 cut(s) 236, 317
BpuMI CCSGG 1 cut(s) 200
BsaBI GATNNNNATC 2 cut(s) 143, 207
BsaJI CCNNGG 2 cut(s) 172, 257
BsaXI ACNNNNNCTCC 4 cut(s) 613, 638, 643, 668
Bsc4I CCNNNNNNNGG 2 cut(s) 639, 640
Bse1I ACTGG 1 cut(s) 782
Bse21I CCTNAGG 1 cut(s) 37
Bse8I GATNNNNATC 2 cut(s) 143, 207
BseBI CCWGG 2 cut(s) 792, 1000
BseDI CCNNGG 2 cut(s) 172, 257
BseGI GGATG 4 cut(s) 208, 772, 875, 971
BseJI GATNNNNATC 2 cut(s) 143, 207
BseLI CCNNNNNNNGG 2 cut(s) 639, 640
BseMII CTCAG 2 cut(s) 51, 935
BseNI ACTGG 1 cut(s) 782
BseRI GAGGAG 2 cut(s) 25, 158
BseSI GKGCMC 1 cut(s) 200
BseXI GCAGC 2 cut(s) 232, 546
BseYI CCCAGC 1 cut(s) 216
BshFI GGCC 4 cut(s) 84, 177, 198, 338
BsiHKAI GWGCWC 2 cut(s) 252, 504
BsiSI CCGG 2 cut(s) 200, 650
BslFI GGGAC 2 cut(s) 341, 370
BslI CCNNNNNNNGG 2 cut(s) 639, 640
BsmFI GGGAC 2 cut(s) 341, 370
BsmI GAATGC 2 cut(s) 18, 584
BsnI GGCC 4 cut(s) 84, 177, 198, 338
Bsp120I GGGCCC 1 cut(s) 196
Bsp1286I GDGCHC 4 cut(s) 200, 252, 366, 504
Bsp1407I TGTACA 1 cut(s) 105
Bsp143I GATC 7 cut(s) 124, 138, 144, 208, 318, 424, 475
Bsp19I CCATGG 2 cut(s) 172, 257
BspACI CCGC 7 cut(s) 133, 339, 354, 386, 597, 603, 884
BspANI GGCC 4 cut(s) 84, 177, 198, 338
BspCNI CTCAG 2 cut(s) 50, 934
BspLI GGNNCC 5 cut(s) 198, 283, 329, 363, 648
BspMI ACCTGC 2 cut(s) 171, 472
BspPI GGATC 1 cut(s) 470
BsrGI TGTACA 1 cut(s) 105
BsrI ACTGG 1 cut(s) 782
BssECI CCNNGG 2 cut(s) 172, 257
BssMI GATC 7 cut(s) 124, 138, 144, 208, 318, 424, 475
BssT1I CCWWGG 2 cut(s) 172, 257
Bst2UI CCWGG 2 cut(s) 792, 1000
Bst4CI ACNGT 2 cut(s) 89, 858
BstAPI GCANNNNNTGC 1 cut(s) 962
BstAUI TGTACA 1 cut(s) 105
BstC8I GCNNGC 4 cut(s) 28, 557, 804, 834
BstDEI CTNAG 3 cut(s) 37, 537, 921
BstDSI CCRYGG 2 cut(s) 172, 257
BstF5I GGATG 4 cut(s) 208, 772, 875, 971
BstH2I RGCGCY 1 cut(s) 292
BstHHI GCGC 1 cut(s) 291
BstKTI GATC 7 cut(s) 127, 141, 147, 211, 321, 427, 478
BstMBI GATC 7 cut(s) 124, 138, 144, 208, 318, 424, 475
BstMWI GCNNNNNNNGC 5 cut(s) 288, 603, 612, 829, 962
BstNI CCWGG 2 cut(s) 792, 1000
BstSCI CCNGG 3 cut(s) 198, 790, 998
BstSLI GKGCMC 1 cut(s) 200
BstV1I GCAGC 2 cut(s) 232, 546
BstX2I RGATCY 1 cut(s) 318
BstYI RGATCY 1 cut(s) 318
Bsu36I CCTNAGG 1 cut(s) 37
BsuRI GGCC 4 cut(s) 84, 177, 198, 338
BtgI CCRYGG 2 cut(s) 172, 257
BtsCI GGATG 4 cut(s) 208, 772, 875, 971
BtsI GCAGTG 1 cut(s) 318
BtsIMutI CAGTG 3 cut(s) 94, 318, 471
BveI ACCTGC 2 cut(s) 171, 472
Cac8I GCNNGC 4 cut(s) 28, 557, 804, 834
CfoI GCGC 1 cut(s) 291
Cfr13I GGNCC 3 cut(s) 196, 197, 328
Csp6I GTAC 5 cut(s) 106, 262, 376, 630, 843
CviAII CATG 7 cut(s) 97, 150, 173, 258, 563, 689, 773
CviQI GTAC 5 cut(s) 106, 262, 376, 630, 843
DdeI CTNAG 3 cut(s) 37, 537, 921
DpnI GATC 7 cut(s) 126, 140, 146, 210, 320, 426, 477
DpnII GATC 7 cut(s) 124, 138, 144, 208, 318, 424, 475
DraIII CACNNNGTG 1 cut(s) 153
Ecl136II GAGCTC 1 cut(s) 250
Eco130I CCWWGG 2 cut(s) 172, 257
Eco24I GRGCYC 3 cut(s) 200, 252, 366
Eco47I GGWCC 1 cut(s) 328
Eco47III AGCGCT 1 cut(s) 290
Eco53kI GAGCTC 1 cut(s) 250
Eco81I CCTNAGG 1 cut(s) 37
EcoICRI GAGCTC 1 cut(s) 250
EcoO109I RGGNCCY 2 cut(s) 196, 328
EcoRII CCWGG 2 cut(s) 790, 998
EcoT14I CCWWGG 2 cut(s) 172, 257
EcoT22I ATGCAT 2 cut(s) 64, 442
EcoT38I GRGCYC 3 cut(s) 200, 252, 366
ErhI CCWWGG 2 cut(s) 172, 257
FaeI CATG 7 cut(s) 100, 153, 176, 261, 566, 692, 776
FaqI GGGAC 2 cut(s) 341, 370
FatI CATG 7 cut(s) 96, 149, 172, 257, 562, 688, 772
FauI CCCGC 1 cut(s) 347
FbaI TGATCA 1 cut(s) 138
FblI GTMKAC 1 cut(s) 93
Fnu4HI GCNGC 6 cut(s) 133, 221, 339, 560, 604, 884
FokI GGATG 4 cut(s) 215, 779, 862, 958
FriOI GRGCYC 3 cut(s) 200, 252, 366
Fsp4HI GCNGC 6 cut(s) 133, 221, 339, 560, 604, 884
FspBI CTAG 2 cut(s) 75, 927
GlaI GCGC 1 cut(s) 290
GluI GCNGC 6 cut(s) 133, 221, 339, 560, 604, 884
GsaI CCCAGC 1 cut(s) 220
GsuI CTGGAG 1 cut(s) 289
HaeII RGCGCY 1 cut(s) 292
HaeIII GGCC 4 cut(s) 84, 177, 198, 338
HapII CCGG 2 cut(s) 200, 650
HhaI GCGC 1 cut(s) 291
Hin1II CATG 7 cut(s) 100, 153, 176, 261, 566, 692, 776
Hin6I GCGC 1 cut(s) 289
HinP1I GCGC 1 cut(s) 289
HinfI GANTC 1 cut(s) 725
HpaII CCGG 2 cut(s) 200, 650
HphI GGTGA 4 cut(s) 53, 196, 434, 846
Hpy166II GTNNAC 2 cut(s) 94, 106
Hpy188I TCNGA 4 cut(s) 129, 373, 924, 976
Hpy188III TCNNGA 3 cut(s) 142, 268, 864
Hpy8I GTNNAC 2 cut(s) 94, 106
Hpy99I CGWCG 1 cut(s) 611
HpyAV CCTTC 2 cut(s) 301, 938
HpyCH4III ACNGT 2 cut(s) 89, 858
HpyCH4IV ACGT 1 cut(s) 381
HpyF10VI GCNNNNNNNGC 5 cut(s) 288, 603, 612, 829, 962
HpyF3I CTNAG 3 cut(s) 37, 537, 921
HpySE526I ACGT 1 cut(s) 381
Hsp92II CATG 7 cut(s) 100, 153, 176, 261, 566, 692, 776
HspAI GCGC 1 cut(s) 289
Ksp22I TGATCA 1 cut(s) 138
Kzo9I GATC 7 cut(s) 124, 138, 144, 208, 318, 424, 475
LmnI GCTCC 5 cut(s) 171, 287, 361, 612, 646
Lsp1109I GCAGC 2 cut(s) 232, 546
LweI GCATC 6 cut(s) 49, 71, 427, 706, 911, 944
MaeI CTAG 2 cut(s) 75, 927
MaeII ACGT 1 cut(s) 381
MaeIII GTNAC 3 cut(s) 184, 395, 852
MalI GATC 7 cut(s) 126, 140, 146, 210, 320, 426, 477
MboI GATC 7 cut(s) 124, 138, 144, 208, 318, 424, 475
MboII GAAGA 3 cut(s) 313, 692, 885
MfeI CAATTG 1 cut(s) 573
MflI RGATCY 1 cut(s) 318
MhlI GDGCHC 4 cut(s) 200, 252, 366, 504
MluCI AATT 4 cut(s) 344, 567, 573, 750
MlyI GAGTC 1 cut(s) 734
MmeI TCCRAC 1 cut(s) 636
Mph1103I ATGCAT 2 cut(s) 64, 442
MseI TTAA 3 cut(s) 50, 797, 951
MslI CAYNNNNRTG 2 cut(s) 101, 591
MspA1I CMGCKG 1 cut(s) 68
MspI CCGG 2 cut(s) 200, 650
MspR9I CCNGG 3 cut(s) 200, 792, 1000
MunI CAATTG 1 cut(s) 573
Mva1269I GAATGC 2 cut(s) 18, 584
MvaI CCWGG 2 cut(s) 792, 1000
MwoI GCNNNNNNNGC 5 cut(s) 288, 603, 612, 829, 962
NciI CCSGG 1 cut(s) 200
NcoI CCATGG 2 cut(s) 172, 257
NdeII GATC 7 cut(s) 124, 138, 144, 208, 318, 424, 475
NlaIII CATG 7 cut(s) 100, 153, 176, 261, 566, 692, 776
NlaIV GGNNCC 5 cut(s) 198, 283, 329, 363, 648
NmuCI GTSAC 3 cut(s) 184, 395, 852
NsiI ATGCAT 2 cut(s) 64, 442
OliI CACNNNNGTG 1 cut(s) 591
PcsI WCGNNNNNNNCGW 1 cut(s) 869
PctI GAATGC 2 cut(s) 18, 584
PfoI TCCNGGA 1 cut(s) 790
PkrI GCNGC 6 cut(s) 134, 222, 340, 561, 605, 885
PleI GAGTC 1 cut(s) 733
PpsI GAGTC 1 cut(s) 733
PpuMI RGGWCCY 1 cut(s) 328
PshBI ATTAAT 1 cut(s) 951
Psp124BI GAGCTC 1 cut(s) 252
Psp5II RGGWCCY 1 cut(s) 328
Psp6I CCWGG 2 cut(s) 790, 998
PspFI CCCAGC 1 cut(s) 216
PspGI CCWGG 2 cut(s) 790, 998
PspN4I GGNNCC 5 cut(s) 198, 283, 329, 363, 648
PspOMI GGGCCC 1 cut(s) 196
PspPI GGNCC 3 cut(s) 196, 197, 328
PspPPI RGGWCCY 1 cut(s) 328
PsuI RGATCY 1 cut(s) 318
PvuII CAGCTG 1 cut(s) 68
RsaI GTAC 5 cut(s) 107, 263, 377, 631, 844
RsaNI GTAC 5 cut(s) 106, 262, 376, 630, 843
RseI CAYNNNNRTG 2 cut(s) 101, 591
SacI GAGCTC 1 cut(s) 252
SaqAI TTAA 3 cut(s) 50, 797, 951
SatI GCNGC 6 cut(s) 133, 221, 339, 560, 604, 884
Sau3AI GATC 7 cut(s) 124, 138, 144, 208, 318, 424, 475
Sau96I GGNCC 3 cut(s) 196, 197, 328
ScaI AGTACT 1 cut(s) 377
SchI GAGTC 1 cut(s) 734
ScrFI CCNGG 3 cut(s) 200, 792, 1000
SduI GDGCHC 4 cut(s) 200, 252, 366, 504
SfaNI GCATC 6 cut(s) 49, 71, 427, 706, 911, 944
SinI GGWCC 1 cut(s) 328
SmiMI CAYNNNNRTG 2 cut(s) 101, 591
SmlI CTYRAG 2 cut(s) 251, 332
SmoI CTYRAG 2 cut(s) 251, 332
SpeI ACTAGT 1 cut(s) 926
Sse9I AATT 4 cut(s) 344, 567, 573, 750
SsiI CCGC 7 cut(s) 133, 339, 354, 386, 597, 603, 884
SspMI CTAG 2 cut(s) 75, 927
SstI GAGCTC 1 cut(s) 252
StyD4I CCNGG 3 cut(s) 198, 790, 998
StyI CCWWGG 2 cut(s) 172, 257
TaaI ACNGT 2 cut(s) 89, 858
TaiI ACGT 1 cut(s) 384
TaqI TCGA 1 cut(s) 863
TasI AATT 4 cut(s) 344, 567, 573, 750
TatI WGTACW 3 cut(s) 105, 375, 842
TauI GCSGC 4 cut(s) 135, 341, 606, 886
Tru1I TTAA 3 cut(s) 50, 797, 951
Tru9I TTAA 3 cut(s) 50, 797, 951
TscAI CASTG 3 cut(s) 94, 318, 478
TseFI GTSAC 3 cut(s) 184, 395, 852
TseI GCWGC 2 cut(s) 220, 559
Tsp45I GTSAC 3 cut(s) 184, 395, 852
TspDTI ATGAA 5 cut(s) 561, 579, 677, 885, 960
TspGWI ACGGA 1 cut(s) 374
TspRI CASTG 3 cut(s) 94, 318, 478
VpaK11BI GGWCC 1 cut(s) 328
VspI ATTAAT 1 cut(s) 951
XapI RAATTY 1 cut(s) 344
XcmI CCANNNNNNNNNTGG 2 cut(s) 264, 292
XmiI GTMKAC 1 cut(s) 93
XspI CTAG 2 cut(s) 75, 927
ZrmI AGTACT 1 cut(s) 377
Zsp2I ATGCAT 2 cut(s) 64, 442
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.