MD14G1170600.v1.1

Plant protein of unknown function (DUF868)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0015623
Physical Location & Seq
Reverse (-)
26487546 .. 26488559
1014 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1170600.v1.1.491

Sequence Viewer

Length: 1014 bp
ATGCAAGACCACATTGGCATTCCTGCTTGCTTCTCATCAGGTGAGAAGTTAAGTGATGATGCTTCGGCTGTGACTAGGTCAGGCCACAGTGTGTGCATGTCAGTGTACAGAGCCAAGATAGCCGGTCAGAGCCGCTTGATCACGATCACATGGTGCAAAAACCTGCTCCTCCACGGCCTGTCGGTGTCCGTGGAGGGTCCGGATGGAGATCAAACCCAGCAGCACACTTGCAAAGTTGAACTCAAGCCGTGGTACTTCTGGAGGAAGCAAGGTTCCAAGCGCTTTGTGGTTGATGAGAAAGCAGTGGAAATCTTCTGGGACCTCAAGGCGGCGAAATTTAACGGCGGGACGGAGCCGAAGTCGGAGTACTACGTGGCGGTTGTTTGTGACGAGGAAGTTGTTTTGGTTCTTGGTGATCTCAAAAAAGATGCTTATAGAAAAGCAGGTTTTAGACCTGCCCTGACTGATCCCACTTTGGTTTCCAAAAAGGAGCACATTTTTGGTAAGAAGAAGTTTTCCACAAAAGCTAAGTTTTATGAGAGAGGCAGATCGCATGAAATTGCAATTGAATGCAAAAACAGAGGTGGTGGCGGCGGCGGTGGCAGCGGGAGCATTGGGAGTGGAAGTACCCTTAATGGGGTGGAGCCAGAGTTGGAGATTAGGGTTGATGGGCATTTGGTGGTTCATGTGAAGCATCTTCAATGGAAGTTTAGAGGTAATGAGTCCATTCACATCAACAAGTTGAGAATTGAGGTTTATTGGGATGTCCATGACTGGCTTTTTAGTCCTGGATTAAGGCATGCATTGTTTATTTTCAAGCCAAATTTGCCTGCTTCAAGTACATCTCTATCGCCATTGTCGAGAACCTCGTCGTCCTCACCGCCATTTTCGTCATTGGCATCAACTCCATTGTCATCTCAGACTAGTTCTGGGTCAGTAGAAGGGATTAATAACGCAACTTCTGCCGGTTCATCTGAGTTTTGCTTGTTTCTTTATGCCTGGAAAGTTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF868

Protein Analysis

338

Amino Acids

37.07

Weight (kDa)

8.93

Isoelectric Point (pI)

48.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF868 PF05910 28 - 335 1.9e-106 Plant protein of unknown function (DUF868)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015623)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g06390
malus_domestica MD06G1165100.v1.1 MD14G1170600.v1.1
prunus_persica Prupe.5G164700_v2.0.a1
pyrus_communis pycom06g14760 pycom14g14240
rosa_chinensis RchiOBHm_Chr7g0192421
rosa_laevigata RLG00000004369
rosa_multiflora Rmu_sc0003108.1_g000024
rosa_roxburghii Rroxscaffold_3G00263020
rosa_rugosa Rorug07G0001500
rosa_samantha Rh7AG125300 Rh7BG127600 Rh7CG129300 Rh7DG129400
rosa_wichuraiana Rw7G010790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 171, 434, 463
AccIII TCCGGA 1 cut(s) 199
AciI CCGC 9 cut(s) 133, 329, 345, 377, 591, 594, 597, 606, 881
AclWI GGATC 1 cut(s) 461
AcsI RAATTY 2 cut(s) 335, 823
AdeI CACNNNGTG 2 cut(s) 91, 153
AfaI GTAC 5 cut(s) 107, 254, 368, 628, 841
AfeI AGCGCT 1 cut(s) 281
AfiI CCNNNNNNNGG 3 cut(s) 328, 636, 637
AgsI TTSAA 6 cut(s) 239, 569, 701, 817, 837, 1010
AhlI ACTAGT 1 cut(s) 923
AjnI CCWGG 2 cut(s) 787, 998
AloI GAACNNNNNNTCC 2 cut(s) 256, 288
AluBI AGCT 1 cut(s) 527
AluI AGCT 1 cut(s) 527
Alw21I GWGCWC 1 cut(s) 495
AlwI GGATC 1 cut(s) 461
Aor13HI TCCGGA 1 cut(s) 199
Aor51HI AGCGCT 1 cut(s) 281
AoxI GGCC 2 cut(s) 82, 175
ApeKI GCWGC 2 cut(s) 220, 603
ApoI RAATTY 2 cut(s) 335, 823
AseI ATTAAT 1 cut(s) 948
AspLEI GCGC 1 cut(s) 282
AspS9I GGNCC 2 cut(s) 197, 319
AsuHPI GGTGA 3 cut(s) 53, 425, 870
AvaII GGWCC 2 cut(s) 197, 319
BarI GAAGNNNNNNTAC 2 cut(s) 350, 382
Bbv12I GWGCWC 1 cut(s) 495
BbvI GCAGC 2 cut(s) 232, 615
BccI CCATC 2 cut(s) 197, 662
BceAI ACGGC 3 cut(s) 190, 232, 358
BciT130I CCWGG 2 cut(s) 789, 1000
BclI TGATCA 1 cut(s) 138
BcuI ACTAGT 1 cut(s) 923
BfaI CTAG 2 cut(s) 75, 924
BfoI RGCGCY 1 cut(s) 283
BfuAI ACCTGC 3 cut(s) 171, 434, 463
BisI GCNGC 6 cut(s) 133, 221, 330, 592, 595, 604
BlsI GCNGC 6 cut(s) 134, 222, 331, 593, 596, 605
BmcAI AGTACT 1 cut(s) 368
Bme1390I CCNGG 2 cut(s) 789, 1000
Bme18I GGWCC 2 cut(s) 197, 319
BmgT120I GGNCC 2 cut(s) 197, 319
BmiI GGNNCC 5 cut(s) 198, 274, 320, 354, 645
BmrFI CCNGG 2 cut(s) 789, 1000
BmsI GCATC 4 cut(s) 49, 418, 703, 908
BpmI CTGGAG 1 cut(s) 280
BpuEI CTTGAG 2 cut(s) 227, 308
BsaAI YACGTR 1 cut(s) 373
BsaBI GATNNNNATC 2 cut(s) 143, 207
BsaJI CCNNGG 3 cut(s) 172, 189, 248
BsaWI WCCGGW 1 cut(s) 199
BsaXI ACNNNNNCTCC 6 cut(s) 344, 374, 610, 635, 640, 665
Bsc4I CCNNNNNNNGG 3 cut(s) 328, 636, 637
Bse118I RCCGGY 2 cut(s) 122, 965
Bse1I ACTGG 1 cut(s) 779
Bse8I GATNNNNATC 2 cut(s) 143, 207
BseAI TCCGGA 1 cut(s) 199
BseBI CCWGG 2 cut(s) 789, 1000
BseDI CCNNGG 3 cut(s) 172, 189, 248
BseGI GGATG 2 cut(s) 208, 769
BseJI GATNNNNATC 2 cut(s) 143, 207
BseLI CCNNNNNNNGG 3 cut(s) 328, 636, 637
BseMII CTCAG 2 cut(s) 932, 966
BseNI ACTGG 1 cut(s) 779
BseRI GAGGAG 1 cut(s) 158
BseXI GCAGC 2 cut(s) 232, 615
BseYI CCCAGC 1 cut(s) 216
BshFI GGCC 2 cut(s) 84, 177
BsiHKAI GWGCWC 1 cut(s) 495
BsiSI CCGG 3 cut(s) 123, 200, 966
BslFI GGGAC 2 cut(s) 332, 361
BslI CCNNNNNNNGG 3 cut(s) 328, 636, 637
BsmFI GGGAC 2 cut(s) 332, 361
BsmI GAATGC 2 cut(s) 18, 575
BsnI GGCC 2 cut(s) 84, 177
Bsp1286I GDGCHC 1 cut(s) 495
Bsp13I TCCGGA 1 cut(s) 199
Bsp1407I TGTACA 1 cut(s) 105
Bsp143I GATC 6 cut(s) 138, 144, 208, 415, 466, 548
BspACI CCGC 9 cut(s) 133, 329, 345, 377, 591, 594, 597, 606, 881
BspANI GGCC 2 cut(s) 84, 177
BspCNI CTCAG 2 cut(s) 931, 967
BspEI TCCGGA 1 cut(s) 199
BspLI GGNNCC 5 cut(s) 198, 274, 320, 354, 645
BspMI ACCTGC 3 cut(s) 171, 434, 463
BspPI GGATC 1 cut(s) 461
BsrFI RCCGGY 2 cut(s) 122, 965
BsrGI TGTACA 1 cut(s) 105
BsrI ACTGG 1 cut(s) 779
BssAI RCCGGY 2 cut(s) 122, 965
BssECI CCNNGG 3 cut(s) 172, 189, 248
BssMI GATC 6 cut(s) 138, 144, 208, 415, 466, 548
Bst2UI CCWGG 2 cut(s) 789, 1000
Bst4CI ACNGT 1 cut(s) 89
BstAPI GCANNNNNTGC 1 cut(s) 962
BstAUI TGTACA 1 cut(s) 105
BstBAI YACGTR 1 cut(s) 373
BstC8I GCNNGC 3 cut(s) 28, 801, 831
BstDEI CTNAG 3 cut(s) 528, 918, 975
BstDSI CCRYGG 3 cut(s) 172, 189, 248
BstF5I GGATG 2 cut(s) 208, 769
BstH2I RGCGCY 1 cut(s) 283
BstHHI GCGC 1 cut(s) 282
BstKTI GATC 6 cut(s) 141, 147, 211, 418, 469, 551
BstMBI GATC 6 cut(s) 138, 144, 208, 415, 466, 548
BstMWI GCNNNNNNNGC 6 cut(s) 119, 600, 603, 609, 826, 962
BstNI CCWGG 2 cut(s) 789, 1000
BstNSI RCATGY 2 cut(s) 100, 803
BstSCI CCNGG 2 cut(s) 787, 998
BstV1I GCAGC 2 cut(s) 232, 615
BsuRI GGCC 2 cut(s) 84, 177
BtgI CCRYGG 3 cut(s) 172, 189, 248
BtsCI GGATG 2 cut(s) 208, 769
BtsI GCAGTG 1 cut(s) 309
BtsIMutI CAGTG 3 cut(s) 94, 108, 309
BveI ACCTGC 3 cut(s) 171, 434, 463
Cac8I GCNNGC 3 cut(s) 28, 801, 831
CfoI GCGC 1 cut(s) 282
Cfr10I RCCGGY 2 cut(s) 122, 965
Cfr13I GGNCC 2 cut(s) 197, 319
Csp6I GTAC 5 cut(s) 106, 253, 367, 627, 840
CviAII CATG 6 cut(s) 97, 150, 554, 686, 770, 800
CviQI GTAC 5 cut(s) 106, 253, 367, 627, 840
DdeI CTNAG 3 cut(s) 528, 918, 975
DpnI GATC 6 cut(s) 140, 146, 210, 417, 468, 550
DpnII GATC 6 cut(s) 138, 144, 208, 415, 466, 548
DraIII CACNNNGTG 2 cut(s) 91, 153
Eco47I GGWCC 2 cut(s) 197, 319
Eco47III AGCGCT 1 cut(s) 281
EcoO109I RGGNCCY 1 cut(s) 319
EcoRII CCWGG 2 cut(s) 787, 998
EcoT22I ATGCAT 1 cut(s) 805
FaeI CATG 6 cut(s) 100, 153, 557, 689, 773, 803
FaiI YATR 9 cut(s) 98, 151, 435, 537, 555, 687, 771, 801, 996
FaqI GGGAC 2 cut(s) 332, 361
FatI CATG 6 cut(s) 96, 149, 553, 685, 769, 799
FauI CCCGC 2 cut(s) 338, 599
FbaI TGATCA 1 cut(s) 138
Fnu4HI GCNGC 6 cut(s) 133, 221, 330, 592, 595, 604
FokI GGATG 2 cut(s) 215, 776
Fsp4HI GCNGC 6 cut(s) 133, 221, 330, 592, 595, 604
FspBI CTAG 2 cut(s) 75, 924
GlaI GCGC 1 cut(s) 281
GluI GCNGC 6 cut(s) 133, 221, 330, 592, 595, 604
GsaI CCCAGC 1 cut(s) 220
GsuI CTGGAG 1 cut(s) 280
HaeII RGCGCY 1 cut(s) 283
HaeIII GGCC 2 cut(s) 84, 177
HapII CCGG 3 cut(s) 123, 200, 966
HhaI GCGC 1 cut(s) 282
Hin1II CATG 6 cut(s) 100, 153, 557, 689, 773, 803
Hin6I GCGC 1 cut(s) 280
HinP1I GCGC 1 cut(s) 280
HinfI GANTC 1 cut(s) 722
HpaII CCGG 3 cut(s) 123, 200, 966
HphI GGTGA 3 cut(s) 53, 425, 870
Hpy166II GTNNAC 1 cut(s) 106
Hpy188I TCNGA 4 cut(s) 129, 364, 921, 976
Hpy188III TCNNGA 4 cut(s) 142, 200, 259, 861
Hpy8I GTNNAC 1 cut(s) 106
Hpy99I CGWCG 1 cut(s) 874
HpyAV CCTTC 1 cut(s) 935
HpyCH4III ACNGT 1 cut(s) 89
HpyCH4IV ACGT 1 cut(s) 372
HpyCH4V TGCA 7 cut(s) 4, 96, 156, 231, 563, 573, 803
HpyF10VI GCNNNNNNNGC 6 cut(s) 119, 600, 603, 609, 826, 962
HpyF3I CTNAG 3 cut(s) 528, 918, 975
HpySE526I ACGT 1 cut(s) 372
Hsp92II CATG 6 cut(s) 100, 153, 557, 689, 773, 803
HspAI GCGC 1 cut(s) 280
Kpn2I TCCGGA 1 cut(s) 199
Ksp22I TGATCA 1 cut(s) 138
Kzo9I GATC 6 cut(s) 138, 144, 208, 415, 466, 548
LmnI GCTCC 5 cut(s) 171, 352, 490, 609, 643
Lsp1109I GCAGC 2 cut(s) 232, 615
LweI GCATC 4 cut(s) 49, 418, 703, 908
MaeI CTAG 2 cut(s) 75, 924
MaeII ACGT 1 cut(s) 372
MaeIII GTNAC 2 cut(s) 70, 386
MalI GATC 6 cut(s) 140, 146, 210, 417, 468, 550
MboI GATC 6 cut(s) 138, 144, 208, 415, 466, 548
MboII GAAGA 3 cut(s) 304, 520, 689
MfeI CAATTG 1 cut(s) 564
MhlI GDGCHC 1 cut(s) 495
MluCI AATT 5 cut(s) 335, 558, 564, 747, 823
MlyI GAGTC 1 cut(s) 731
MmeI TCCRAC 2 cut(s) 342, 633
Mph1103I ATGCAT 1 cut(s) 805
MroI TCCGGA 1 cut(s) 199
MseI TTAA 5 cut(s) 50, 339, 633, 794, 948
MslI CAYNNNNRTG 1 cut(s) 101
MspA1I CMGCKG 1 cut(s) 606
MspI CCGG 3 cut(s) 123, 200, 966
MspR9I CCNGG 2 cut(s) 789, 1000
MunI CAATTG 1 cut(s) 564
Mva1269I GAATGC 2 cut(s) 18, 575
MvaI CCWGG 2 cut(s) 789, 1000
MwoI GCNNNNNNNGC 6 cut(s) 119, 600, 603, 609, 826, 962
NdeII GATC 6 cut(s) 138, 144, 208, 415, 466, 548
NlaIII CATG 6 cut(s) 100, 153, 557, 689, 773, 803
NlaIV GGNNCC 5 cut(s) 198, 274, 320, 354, 645
NmuCI GTSAC 2 cut(s) 70, 386
NsiI ATGCAT 1 cut(s) 805
NspI RCATGY 2 cut(s) 100, 803
PaeI GCATGC 1 cut(s) 803
PcsI WCGNNNNNNNCGW 2 cut(s) 857, 866
PctI GAATGC 2 cut(s) 18, 575
PflFI GACNNNGTC 1 cut(s) 76
PfoI TCCNGGA 1 cut(s) 787
PkrI GCNGC 6 cut(s) 134, 222, 331, 593, 596, 605
PleI GAGTC 1 cut(s) 730
PpsI GAGTC 1 cut(s) 730
Ppu21I YACGTR 1 cut(s) 373
PpuMI RGGWCCY 1 cut(s) 319
PshBI ATTAAT 1 cut(s) 948
Psp5II RGGWCCY 1 cut(s) 319
Psp6I CCWGG 2 cut(s) 787, 998
PspFI CCCAGC 1 cut(s) 216
PspGI CCWGG 2 cut(s) 787, 998
PspN4I GGNNCC 5 cut(s) 198, 274, 320, 354, 645
PspPI GGNCC 2 cut(s) 197, 319
PspPPI RGGWCCY 1 cut(s) 319
PsyI GACNNNGTC 1 cut(s) 76
RsaI GTAC 5 cut(s) 107, 254, 368, 628, 841
RsaNI GTAC 5 cut(s) 106, 253, 367, 627, 840
RseI CAYNNNNRTG 1 cut(s) 101
SaqAI TTAA 5 cut(s) 50, 339, 633, 794, 948
SatI GCNGC 6 cut(s) 133, 221, 330, 592, 595, 604
Sau3AI GATC 6 cut(s) 138, 144, 208, 415, 466, 548
Sau96I GGNCC 2 cut(s) 197, 319
ScaI AGTACT 1 cut(s) 368
SchI GAGTC 1 cut(s) 731
ScrFI CCNGG 2 cut(s) 789, 1000
SduI GDGCHC 1 cut(s) 495
SfaNI GCATC 4 cut(s) 49, 418, 703, 908
SinI GGWCC 2 cut(s) 197, 319
SmiMI CAYNNNNRTG 1 cut(s) 101
SmlI CTYRAG 2 cut(s) 242, 323
SmoI CTYRAG 2 cut(s) 242, 323
SpeI ACTAGT 1 cut(s) 923
SphI GCATGC 1 cut(s) 803
Sse9I AATT 5 cut(s) 335, 558, 564, 747, 823
SsiI CCGC 9 cut(s) 133, 329, 345, 377, 591, 594, 597, 606, 881
SspMI CTAG 2 cut(s) 75, 924
StyD4I CCNGG 2 cut(s) 787, 998
TaaI ACNGT 1 cut(s) 89
TaiI ACGT 1 cut(s) 375
TaqI TCGA 1 cut(s) 860
TasI AATT 5 cut(s) 335, 558, 564, 747, 823
TatI WGTACW 3 cut(s) 105, 366, 839
TauI GCSGC 4 cut(s) 135, 332, 594, 597
Tru1I TTAA 5 cut(s) 50, 339, 633, 794, 948
Tru9I TTAA 5 cut(s) 50, 339, 633, 794, 948
TscAI CASTG 3 cut(s) 94, 108, 309
TseFI GTSAC 2 cut(s) 70, 386
TseI GCWGC 2 cut(s) 220, 603
Tsp45I GTSAC 2 cut(s) 70, 386
TspDTI ATGAA 3 cut(s) 570, 674, 960
TspGWI ACGGA 2 cut(s) 178, 365
TspRI CASTG 3 cut(s) 94, 108, 309
Tth111I GACNNNGTC 1 cut(s) 76
VpaK11BI GGWCC 2 cut(s) 197, 319
VspI ATTAAT 1 cut(s) 948
XapI RAATTY 2 cut(s) 335, 823
XceI RCATGY 2 cut(s) 100, 803
XcmI CCANNNNNNNNNTGG 1 cut(s) 283
XspI CTAG 2 cut(s) 75, 924
ZrmI AGTACT 1 cut(s) 368
Zsp2I ATGCAT 1 cut(s) 805
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.