pycom14g14240

Plant protein of unknown function (DUF868)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0015623
Physical Location & Seq
Reverse (-)
17011419 .. 17012423
1005 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g14240.1

Sequence Viewer

Length: 1005 bp
ATGCAAGACCACATTGGCATTCCTGCTTGCTTCTCATCAGGTGAGAAGTTAAGTGATGATGCTTCGGCTGTGACTAGGTCAGGCCACAGTGTGTGCATGTCAGTGTACAGAGCCAAGATAGCCGGTCAGAGCCGCTTGATCACGATCACATGGTGCAAAAACCTGCTCCTCCACGGCCTGTCGGTGTCCGTGGAGGGCCCGGATGGAGATCAAACCCAGCAGCACACCTGCAAAGTTGAGCTCAAGCCGTGGTACTTCTGGAGGAAGCAAGGCTCCAAGCGCTTTGTGGTTGATGAGAAAGCAGTGGAAATCTTCTGGGACCTCAAGGCGGCGAAATTCAACGGCGGGACGGAGCCCAAATCGGAGTACTACGTGGCGGTTGTTTGTGACGAGGAAGTTGTTTTGGTTCTTGGTGATCTCAAAAAAGATGCTTATAGAAAAACAGGTTTTAGACCTGCCCTGACTGATCCCACTTTGGTTTCCAAAAAGGAGCACATTTTTGGTAAGAAAAAGTTTTCCACAAAAGCTAAGTTTTATGAGAGAGGCAGGTCGCATGAAATTGCAATTGAATGCAAAAACAGAGGCGGCGGCGGCGGCGGGAGCATTGGGAGTGGAAATACTCTAAATGCGGTGGAGCCGGAGTTGGAGATTAGGGTTGATGGGCATTTGGTGGTTCATGTGAAGCATCTTCAATGGAAGTTTAGAGGTAATGAGTCCATTCATATCAACAAGTTGAGAATTGAGGTTTATTGGGATGTCCATGACTGGCTTTTTAGTCCTGGATTAAGGCATGCATTGTTTATTTTCAAGCCAAATTTGCCTGCTTCAAGTACATCTCTGTCGCCATTGTCGAGAACCTCGTCGTCCTCGCCGCCATTTTCGTCATTGGCATCAACTCCATTGTCATCTCAGACTAGTTCTGGGTCAGTAGAAGGGATTAACAATGTAACTTCTGCCGGTTCATCTGAGTTTTGCTTGTTTCTTTATGCCTGGAAAGTTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF868

Protein Analysis

335

Amino Acids

36.97

Weight (kDa)

8.93

Isoelectric Point (pI)

47.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF868 PF05910 28 - 332 5.1e-107 Plant protein of unknown function (DUF868)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015623)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g06390
malus_domestica MD06G1165100.v1.1 MD14G1170600.v1.1
prunus_persica Prupe.5G164700_v2.0.a1
pyrus_communis pycom06g14760 pycom14g14240
rosa_chinensis RchiOBHm_Chr7g0192421
rosa_laevigata RLG00000004369
rosa_multiflora Rmu_sc0003108.1_g000024
rosa_roxburghii Rroxscaffold_3G00263020
rosa_rugosa Rorug07G0001500
rosa_samantha Rh7AG125300 Rh7BG127600 Rh7CG129300 Rh7DG129400
rosa_wichuraiana Rw7G010790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 236
Acc36I ACCTGC 4 cut(s) 171, 236, 463, 537
AclWI GGATC 1 cut(s) 461
AcsI RAATTY 2 cut(s) 335, 814
AdeI CACNNNGTG 2 cut(s) 91, 153
AfaI GTAC 4 cut(s) 107, 254, 368, 832
AfeI AGCGCT 1 cut(s) 281
AfiI CCNNNNNNNGG 1 cut(s) 328
AgsI TTSAA 6 cut(s) 340, 569, 692, 808, 828, 1001
AhlI ACTAGT 1 cut(s) 914
AjnI CCWGG 2 cut(s) 778, 989
AluBI AGCT 2 cut(s) 241, 527
AluI AGCT 2 cut(s) 241, 527
Alw21I GWGCWC 2 cut(s) 243, 495
AlwI GGATC 1 cut(s) 461
Aor51HI AGCGCT 1 cut(s) 281
AoxI GGCC 3 cut(s) 82, 175, 196
ApaI GGGCCC 1 cut(s) 200
ApeKI GCWGC 1 cut(s) 220
ApoI RAATTY 2 cut(s) 335, 814
AspLEI GCGC 1 cut(s) 282
AspS9I GGNCC 3 cut(s) 196, 197, 319
AsuC2I CCSGG 1 cut(s) 200
AsuHPI GGTGA 2 cut(s) 53, 425
AvaII GGWCC 1 cut(s) 319
BaeGI GKGCMC 1 cut(s) 200
BanII GRGCYC 3 cut(s) 200, 243, 357
Bbv12I GWGCWC 2 cut(s) 243, 495
BbvI GCAGC 1 cut(s) 232
BccI CCATC 2 cut(s) 197, 653
BceAI ACGGC 3 cut(s) 190, 232, 358
BciT130I CCWGG 2 cut(s) 780, 991
BclI TGATCA 1 cut(s) 138
BcnI CCSGG 1 cut(s) 200
BcuI ACTAGT 1 cut(s) 914
BfaI CTAG 2 cut(s) 75, 915
BfoI RGCGCY 1 cut(s) 283
BfuAI ACCTGC 4 cut(s) 171, 236, 463, 537
BisI GCNGC 8 cut(s) 133, 221, 330, 586, 589, 592, 595, 872
BlsI GCNGC 8 cut(s) 134, 222, 331, 587, 590, 593, 596, 873
BmcAI AGTACT 1 cut(s) 368
Bme1390I CCNGG 3 cut(s) 200, 780, 991
Bme18I GGWCC 1 cut(s) 319
BmgT120I GGNCC 3 cut(s) 196, 197, 319
BmiI GGNNCC 5 cut(s) 198, 274, 320, 354, 636
BmrFI CCNGG 3 cut(s) 200, 780, 991
BmsI GCATC 4 cut(s) 49, 418, 694, 899
BpmI CTGGAG 1 cut(s) 280
BpuEI CTTGAG 2 cut(s) 227, 308
BpuMI CCSGG 1 cut(s) 200
BsaAI YACGTR 1 cut(s) 373
BsaBI GATNNNNATC 2 cut(s) 143, 207
BsaJI CCNNGG 3 cut(s) 172, 189, 248
BsaXI ACNNNNNCTCC 2 cut(s) 626, 656
Bsc4I CCNNNNNNNGG 1 cut(s) 328
Bse118I RCCGGY 2 cut(s) 122, 956
Bse1I ACTGG 1 cut(s) 770
Bse8I GATNNNNATC 2 cut(s) 143, 207
BseBI CCWGG 2 cut(s) 780, 991
BseDI CCNNGG 3 cut(s) 172, 189, 248
BseGI GGATG 2 cut(s) 208, 760
BseJI GATNNNNATC 2 cut(s) 143, 207
BseLI CCNNNNNNNGG 1 cut(s) 328
BseMII CTCAG 2 cut(s) 923, 957
BseNI ACTGG 1 cut(s) 770
BseRI GAGGAG 1 cut(s) 158
BseSI GKGCMC 1 cut(s) 200
BseXI GCAGC 1 cut(s) 232
BseYI CCCAGC 1 cut(s) 216
BshFI GGCC 3 cut(s) 84, 177, 198
BsiHKAI GWGCWC 2 cut(s) 243, 495
BsiSI CCGG 4 cut(s) 123, 200, 638, 957
BslFI GGGAC 2 cut(s) 332, 361
BslI CCNNNNNNNGG 1 cut(s) 328
BsmFI GGGAC 2 cut(s) 332, 361
BsmI GAATGC 2 cut(s) 18, 575
BsnI GGCC 3 cut(s) 84, 177, 198
Bsp120I GGGCCC 1 cut(s) 196
Bsp1286I GDGCHC 4 cut(s) 200, 243, 357, 495
Bsp1407I TGTACA 1 cut(s) 105
Bsp143I GATC 5 cut(s) 138, 144, 208, 415, 466
BspANI GGCC 3 cut(s) 84, 177, 198
BspCNI CTCAG 2 cut(s) 922, 958
BspLI GGNNCC 5 cut(s) 198, 274, 320, 354, 636
BspMI ACCTGC 4 cut(s) 171, 236, 463, 537
BspPI GGATC 1 cut(s) 461
BsrFI RCCGGY 2 cut(s) 122, 956
BsrGI TGTACA 1 cut(s) 105
BsrI ACTGG 1 cut(s) 770
BssAI RCCGGY 2 cut(s) 122, 956
BssECI CCNNGG 3 cut(s) 172, 189, 248
BssMI GATC 5 cut(s) 138, 144, 208, 415, 466
Bst2UI CCWGG 2 cut(s) 780, 991
Bst4CI ACNGT 1 cut(s) 89
BstAUI TGTACA 1 cut(s) 105
BstBAI YACGTR 1 cut(s) 373
BstC8I GCNNGC 3 cut(s) 28, 792, 822
BstDEI CTNAG 3 cut(s) 528, 909, 966
BstDSI CCRYGG 3 cut(s) 172, 189, 248
BstF5I GGATG 2 cut(s) 208, 760
BstH2I RGCGCY 1 cut(s) 283
BstHHI GCGC 1 cut(s) 282
BstKTI GATC 5 cut(s) 141, 147, 211, 418, 469
BstMBI GATC 5 cut(s) 138, 144, 208, 415, 466
BstMWI GCNNNNNNNGC 6 cut(s) 119, 279, 591, 594, 600, 817
BstNI CCWGG 2 cut(s) 780, 991
BstNSI RCATGY 2 cut(s) 100, 794
BstSCI CCNGG 3 cut(s) 198, 778, 989
BstSLI GKGCMC 1 cut(s) 200
BstV1I GCAGC 1 cut(s) 232
BsuRI GGCC 3 cut(s) 84, 177, 198
BtgI CCRYGG 3 cut(s) 172, 189, 248
BtsCI GGATG 2 cut(s) 208, 760
BtsI GCAGTG 1 cut(s) 309
BtsIMutI CAGTG 3 cut(s) 94, 108, 309
BveI ACCTGC 4 cut(s) 171, 236, 463, 537
Cac8I GCNNGC 3 cut(s) 28, 792, 822
CfoI GCGC 1 cut(s) 282
Cfr10I RCCGGY 2 cut(s) 122, 956
Cfr13I GGNCC 3 cut(s) 196, 197, 319
Csp6I GTAC 4 cut(s) 106, 253, 367, 831
CviAII CATG 6 cut(s) 97, 150, 554, 677, 761, 791
CviQI GTAC 4 cut(s) 106, 253, 367, 831
DdeI CTNAG 3 cut(s) 528, 909, 966
DpnI GATC 5 cut(s) 140, 146, 210, 417, 468
DpnII GATC 5 cut(s) 138, 144, 208, 415, 466
DraIII CACNNNGTG 2 cut(s) 91, 153
Ecl136II GAGCTC 1 cut(s) 241
Eco24I GRGCYC 3 cut(s) 200, 243, 357
Eco47I GGWCC 1 cut(s) 319
Eco47III AGCGCT 1 cut(s) 281
Eco53kI GAGCTC 1 cut(s) 241
EcoICRI GAGCTC 1 cut(s) 241
EcoO109I RGGNCCY 2 cut(s) 196, 319
EcoRII CCWGG 2 cut(s) 778, 989
EcoT22I ATGCAT 1 cut(s) 796
EcoT38I GRGCYC 3 cut(s) 200, 243, 357
FaeI CATG 6 cut(s) 100, 153, 557, 680, 764, 794
FaqI GGGAC 2 cut(s) 332, 361
FatI CATG 6 cut(s) 96, 149, 553, 676, 760, 790
FauI CCCGC 2 cut(s) 338, 590
FbaI TGATCA 1 cut(s) 138
Fnu4HI GCNGC 8 cut(s) 133, 221, 330, 586, 589, 592, 595, 872
FokI GGATG 2 cut(s) 215, 767
FriOI GRGCYC 3 cut(s) 200, 243, 357
Fsp4HI GCNGC 8 cut(s) 133, 221, 330, 586, 589, 592, 595, 872
FspBI CTAG 2 cut(s) 75, 915
GlaI GCGC 1 cut(s) 281
GluI GCNGC 8 cut(s) 133, 221, 330, 586, 589, 592, 595, 872
GsaI CCCAGC 1 cut(s) 220
GsuI CTGGAG 1 cut(s) 280
HaeII RGCGCY 1 cut(s) 283
HaeIII GGCC 3 cut(s) 84, 177, 198
HapII CCGG 4 cut(s) 123, 200, 638, 957
HhaI GCGC 1 cut(s) 282
Hin1II CATG 6 cut(s) 100, 153, 557, 680, 764, 794
Hin6I GCGC 1 cut(s) 280
HinP1I GCGC 1 cut(s) 280
HinfI GANTC 1 cut(s) 713
HpaII CCGG 4 cut(s) 123, 200, 638, 957
HphI GGTGA 2 cut(s) 53, 425
Hpy166II GTNNAC 1 cut(s) 106
Hpy188I TCNGA 4 cut(s) 129, 364, 912, 967
Hpy188III TCNNGA 3 cut(s) 142, 259, 852
Hpy8I GTNNAC 1 cut(s) 106
Hpy99I CGWCG 1 cut(s) 865
HpyAV CCTTC 1 cut(s) 926
HpyCH4III ACNGT 1 cut(s) 89
HpyCH4IV ACGT 1 cut(s) 372
HpyCH4V TGCA 7 cut(s) 4, 96, 156, 231, 563, 573, 794
HpyF10VI GCNNNNNNNGC 6 cut(s) 119, 279, 591, 594, 600, 817
HpyF3I CTNAG 3 cut(s) 528, 909, 966
HpySE526I ACGT 1 cut(s) 372
Hsp92II CATG 6 cut(s) 100, 153, 557, 680, 764, 794
HspAI GCGC 1 cut(s) 280
Ksp22I TGATCA 1 cut(s) 138
Kzo9I GATC 5 cut(s) 138, 144, 208, 415, 466
LmnI GCTCC 6 cut(s) 171, 278, 352, 490, 600, 634
Lsp1109I GCAGC 1 cut(s) 232
LweI GCATC 4 cut(s) 49, 418, 694, 899
MaeI CTAG 2 cut(s) 75, 915
MaeII ACGT 1 cut(s) 372
MaeIII GTNAC 3 cut(s) 70, 386, 946
MalI GATC 5 cut(s) 140, 146, 210, 417, 468
MboI GATC 5 cut(s) 138, 144, 208, 415, 466
MboII GAAGA 2 cut(s) 304, 680
MfeI CAATTG 1 cut(s) 564
MhlI GDGCHC 4 cut(s) 200, 243, 357, 495
MluCI AATT 5 cut(s) 335, 558, 564, 738, 814
MlyI GAGTC 1 cut(s) 722
MmeI TCCRAC 1 cut(s) 624
Mph1103I ATGCAT 1 cut(s) 796
MseI TTAA 3 cut(s) 50, 785, 939
MslI CAYNNNNRTG 1 cut(s) 101
MspI CCGG 4 cut(s) 123, 200, 638, 957
MspR9I CCNGG 3 cut(s) 200, 780, 991
MunI CAATTG 1 cut(s) 564
Mva1269I GAATGC 2 cut(s) 18, 575
MvaI CCWGG 2 cut(s) 780, 991
MwoI GCNNNNNNNGC 6 cut(s) 119, 279, 591, 594, 600, 817
NciI CCSGG 1 cut(s) 200
NdeII GATC 5 cut(s) 138, 144, 208, 415, 466
NlaIII CATG 6 cut(s) 100, 153, 557, 680, 764, 794
NlaIV GGNNCC 5 cut(s) 198, 274, 320, 354, 636
NmuCI GTSAC 2 cut(s) 70, 386
NsiI ATGCAT 1 cut(s) 796
NspI RCATGY 2 cut(s) 100, 794
PaeI GCATGC 1 cut(s) 794
PaqCI CACCTGC 1 cut(s) 236
PcsI WCGNNNNNNNCGW 2 cut(s) 848, 857
PctI GAATGC 2 cut(s) 18, 575
PflFI GACNNNGTC 1 cut(s) 76
PfoI TCCNGGA 1 cut(s) 778
PkrI GCNGC 8 cut(s) 134, 222, 331, 587, 590, 593, 596, 873
PleI GAGTC 1 cut(s) 721
PpsI GAGTC 1 cut(s) 721
Ppu21I YACGTR 1 cut(s) 373
PpuMI RGGWCCY 1 cut(s) 319
Psp124BI GAGCTC 1 cut(s) 243
Psp5II RGGWCCY 1 cut(s) 319
Psp6I CCWGG 2 cut(s) 778, 989
PspFI CCCAGC 1 cut(s) 216
PspGI CCWGG 2 cut(s) 778, 989
PspN4I GGNNCC 5 cut(s) 198, 274, 320, 354, 636
PspOMI GGGCCC 1 cut(s) 196
PspPI GGNCC 3 cut(s) 196, 197, 319
PspPPI RGGWCCY 1 cut(s) 319
PsyI GACNNNGTC 1 cut(s) 76
RsaI GTAC 4 cut(s) 107, 254, 368, 832
RsaNI GTAC 4 cut(s) 106, 253, 367, 831
RseI CAYNNNNRTG 1 cut(s) 101
SacI GAGCTC 1 cut(s) 243
SaqAI TTAA 3 cut(s) 50, 785, 939
SatI GCNGC 8 cut(s) 133, 221, 330, 586, 589, 592, 595, 872
Sau3AI GATC 5 cut(s) 138, 144, 208, 415, 466
Sau96I GGNCC 3 cut(s) 196, 197, 319
ScaI AGTACT 1 cut(s) 368
SchI GAGTC 1 cut(s) 722
ScrFI CCNGG 3 cut(s) 200, 780, 991
SduI GDGCHC 4 cut(s) 200, 243, 357, 495
SfaNI GCATC 4 cut(s) 49, 418, 694, 899
SinI GGWCC 1 cut(s) 319
SmiMI CAYNNNNRTG 1 cut(s) 101
SmlI CTYRAG 2 cut(s) 242, 323
SmoI CTYRAG 2 cut(s) 242, 323
SpeI ACTAGT 1 cut(s) 914
SphI GCATGC 1 cut(s) 794
Sse9I AATT 5 cut(s) 335, 558, 564, 738, 814
SspMI CTAG 2 cut(s) 75, 915
SstI GAGCTC 1 cut(s) 243
StyD4I CCNGG 3 cut(s) 198, 778, 989
TaaI ACNGT 1 cut(s) 89
TaiI ACGT 1 cut(s) 375
TaqI TCGA 1 cut(s) 851
TasI AATT 5 cut(s) 335, 558, 564, 738, 814
TatI WGTACW 3 cut(s) 105, 366, 830
TauI GCSGC 7 cut(s) 135, 332, 588, 591, 594, 597, 874
Tru1I TTAA 3 cut(s) 50, 785, 939
Tru9I TTAA 3 cut(s) 50, 785, 939
TscAI CASTG 3 cut(s) 94, 108, 309
TseFI GTSAC 2 cut(s) 70, 386
TseI GCWGC 1 cut(s) 220
Tsp45I GTSAC 2 cut(s) 70, 386
TspDTI ATGAA 4 cut(s) 570, 665, 710, 951
TspGWI ACGGA 2 cut(s) 178, 365
TspRI CASTG 3 cut(s) 94, 108, 309
Tth111I GACNNNGTC 1 cut(s) 76
VpaK11BI GGWCC 1 cut(s) 319
XapI RAATTY 2 cut(s) 335, 814
XceI RCATGY 2 cut(s) 100, 794
XcmI CCANNNNNNNNNTGG 1 cut(s) 283
XspI CTAG 2 cut(s) 75, 915
ZrmI AGTACT 1 cut(s) 368
Zsp2I ATGCAT 1 cut(s) 796
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.