Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0005996

Physical Location & Seq

Genomic Coordinates Forward (+)

30684800 .. 30688974

Gene Structure 4175 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1166600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1932 bp

ATGTACGCCTCCGACAATCGATACGCCGACGTCAACTCCTACCGTGAGCACCGCAGTGACCTAGTGGGTCCAGCGCCTGCGGTGGCGCCACAGTTAGGCACCGCCTATGGCCGTGGCGGTGGGGAAGCTCCGTATGGGGGCCCGCCGCCTGCGGTTGCTTCATATTCGGTAAGAGTGGGTGGACCGACATTGGGTCCAGGAGACTTTAATGGGTACCCGCCGTTCCAGCCCCCTTTCGAGCGGTTCAATATCGGCCGAGGTGGTGGTAATGGGAGCTTTGGTGGTAGAGCGTCGAATGGTCATGTTGGTGGTGGAAGGGGTCGTGGTGGTATTGGTGGTGGTGGGAGATTTGGTGGCGGTGGCAGGGGTTTTGATGGAGGCCGTGGTGGCGGTAGGAGCTTTGGTGGTAGTCGTGGTGGAGGTGCGGGTTTTGGCGGTGGGCGTGGAGTTGGTGGAAGAGGAGGAGGTAGGCATGGTGGCTCGTCAAGAGGAGACTTGGATAATATTATGCTTCCCAAGCAAGATTTTGGAAACTTGGTACCTTTTGAGAAGAACTTCTATGCTGAAAGTCCTTCTGTGAGGGCAATGTCAGAACATGATGTTATGGTGTATCGTACGAGGCGGGAGATTACCACCGAAGGGCATGATATCCCAAAACCGATTCAGATGTTTGAGGACACGGATTTCCCTGATTACTGCCTAGAGGTGATTGCGAAATTGGGTTTTGTTGAACCAACACCAATTCAGGCTCAAGGATGGCCAATGGCATTAAAGGGTAGAGATTTAATTGGCATTGCCGAGACTGGTTCTGGTAAGACTTTGTCTTATCTGCTGCCGGCGCTGGTACATATTAGCGCACAGCCTCGGTTAGCTCAGGGAGAAGGTCCTATGGTGTTAGTATTAGCACCTACTCGAGAATTGGCAGTTCAAATTCAAGAAGAAGCTTTAAAATTTGGTTTACGTTCCAATATTAGGAGTACTTGCATATATGGCGGTGCTCCAAAAGGACCCCAAATACGCGATCTTAAAAGAGGGGTTGAGATTGTGATAGCTACGCCTGGCCGACTGATAGATATGTTGGAAGCTCAGCATACAAATTTGCGAAGAGTGACTTACCTAGTTTTAGATGAGGCTGATAGGATGTTGGACATGGGATTTGAGCCGCAGATAAGGAAAATTGTTTCTCAAACCCGACCAGATCGGCAGACTTTGTATTGGAGTGCCACGTGGCCAAGGGAGGTTGAAAGTTTAGCAAGGCAGTTTTTACGTAACCCATATAAGGTAATCATTGGATCAGCAAGCCTTAAAGCAAACCAATCCATAAACCAAGTTGTTGATGTTGTGACGGAGGTGGAGAAATACAATAGGCTGATCAAATTGCTCAAAGAAGCGATGGTTGGGAGCCGAATTCTGATTTTTGTGGAGACGAAAAAGGGATGCGACCACGTTACTAAACAATTGAGGATGGATGGATGGCCAGCTCTATCCATCCACGGTGATAAAAACCAGGCCGAAAGGGACTGGGTCTTGGCTGAGTTTAAAAGTGGCAGGAATCCTATAATGACTGCCACTGATGTGGCTGCGCGAGGTCTTGATGTGAAGGACATAAAATGTGTGATCAATTATGATTTTCCATCAAGCCTTGAGGATTATGTTCACAGAATCGGACGAACTGGACGTGCAGGAGCAGCAGGAACTGCTTTAACTTTTTTCACGCACGCAAATGCAAAATTCACTAGGGATCTAATCAAGATCCTTCAAGAAGCAGGTCAGGTTGTGAGCCCTGCATTAGCTGCAATGGCCCGATCAAGTGGTAGTTTCGGAGGATCCGGTGGGAACTTCCGCAATAGAGGACGAGGAGGAGGGTTTGGGAACCGGGGCATGAGTTCTGGTTCAAATACTGTTCCTATCGGTTACAAGAGACCATGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003729 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005829 GO:0006139 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010501 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko03040 ko05202 ko05205 map03040 map05202 map05205

Pfam Domains

Protein Families

DEAD Helicase_C

Protein Analysis

644

Amino Acids

69.0

Weight (kDa)

9.73

Isoelectric Point (pI)

39.29

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	246 - 416	3.9e-49	DEAD/DEAH box helicase
Helicase_C	PF00271	452 - 562	1.1e-31	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005996)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G63120 AT5G63120 AT5G63120 AT5G63120 AT5G63120
fragaria_vesca	FvH4_5g06110 FvH4_5g06110 FvH4_5g06110 FvH4_5g06110 FvH4_5g06110 FvH4_5g06110
malus_domestica	MD06G1166600.v1.1 MD14G1172300.v1.1
prunus_persica	Prupe.5G166600_v2.0.a1 Prupe.5G166600_v2.0.a1 Prupe.5G166600_v2.0.a1
pyrus_communis	pycom06g14930 pycom14g14390
rosa_chinensis	RchiOBHm_Chr7g0192711
rosa_laevigata	RLG00000004335
rosa_multiflora	Rmu_sc0002040.1_g000012
rosa_roxburghii	Rroxscaffold_3G00262730
rosa_rugosa	Rorug07G0004000 Rorug07G0004100
rosa_samantha	Rh7CG133900 Rh7DG131300
rosa_wichuraiana	Rw7G011040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	33
Acc36I	ACCTGC	1 cut(s)	1757
Acc65I	GGTACC	2 cut(s)	213, 538
AccB1I	GGYRCC	4 cut(s)	85, 98, 213, 538
AccBSI	CCGCTC	1 cut(s)	241
AccII	CGCG	2 cut(s)	1020, 1585
AclWI	GGATC	5 cut(s)	1300, 1747, 1749, 1821, 1834
AcoI	YGGCCR	6 cut(s)	109, 253, 758, 1060, 1229, 1475
AcsI	RAATTY	5 cut(s)	930, 950, 1096, 1407, 1730
AcvI	CACGTG	1 cut(s)	1227
AcyI	GRCGYC	2 cut(s)	30, 86
AfaI	GTAC	6 cut(s)	5, 215, 540, 616, 846, 979
AfiI	CCNNNNNNNGG	6 cut(s)	95, 137, 191, 639, 972, 1279
AgsI	TTSAA	7 cut(s)	247, 731, 929, 935, 1244, 1760, 1896
AhdI	GACNNNNNGTC	1 cut(s)	192
AjiI	CACGTC	1 cut(s)	1679
AjnI	CCWGG	3 cut(s)	196, 1057, 1506
AjuI	GAANNNNNNNTTGG	2 cut(s)	1380, 1412
AleI	CACNNNNGTG	2 cut(s)	54, 1574
AluBI	AGCT	9 cut(s)	128, 276, 399, 872, 944, 1052, 1085, 1481, 1793
AluI	AGCT	9 cut(s)	128, 276, 399, 872, 944, 1052, 1085, 1481, 1793
Alw21I	GWGCWC	2 cut(s)	51, 1000
Alw26I	GTCTC	5 cut(s)	195, 486, 794, 1418, 1915
AlwI	GGATC	5 cut(s)	1300, 1747, 1749, 1821, 1834
AlwNI	CAGNNNCTG	2 cut(s)	77, 1697
Ama87I	CYCGRG	1 cut(s)	912
ApaI	GGGCCC	1 cut(s)	143
ApeKI	GCWGC	4 cut(s)	832, 1580, 1688, 1793
ApoI	RAATTY	5 cut(s)	930, 950, 1096, 1407, 1730
ArsI	GACNNNNNNTTYG	2 cut(s)	1139, 1171
Asp700I	GAANNNNTTC	1 cut(s)	554
Asp718I	GGTACC	2 cut(s)	213, 538
AspLEI	GCGC	5 cut(s)	76, 88, 841, 857, 1585
AspS9I	GGNCC	8 cut(s)	68, 139, 140, 182, 194, 884, 1007, 1801
AsuC2I	CCSGG	1 cut(s)	1877
AsuHPI	GGTGA	2 cut(s)	718, 1508
AvaI	CYCGRG	1 cut(s)	912
AvaII	GGWCC	5 cut(s)	68, 182, 194, 884, 1007
BaeGI	GKGCMC	1 cut(s)	143
BalI	TGGCCA	3 cut(s)	760, 1231, 1477
BamHI	GGATCC	1 cut(s)	1826
BanI	GGYRCC	4 cut(s)	85, 98, 213, 538
BanII	GRGCYC	2 cut(s)	143, 1784
BbrPI	CACGTG	1 cut(s)	1227
Bbv12I	GWGCWC	2 cut(s)	51, 1000
BbvI	GCAGC	4 cut(s)	819, 1567, 1700, 1780
BccI	CCATC	8 cut(s)	368, 750, 1387, 1459, 1463, 1467, 1496, 1642
BceAI	ACGGC	3 cut(s)	96, 205, 366
BciT130I	CCWGG	3 cut(s)	198, 1059, 1508
BclI	TGATCA	2 cut(s)	1371, 1617
BcnI	CCSGG	1 cut(s)	1877
BcoDI	GTCTC	5 cut(s)	195, 486, 794, 1418, 1915
BfaI	CTAG	4 cut(s)	62, 701, 1118, 1737
BfoI	RGCGCY	3 cut(s)	77, 89, 842
BfuAI	ACCTGC	1 cut(s)	1757
BglI	GCCNNNNNGGC	1 cut(s)	387
BisI	GCNGC	6 cut(s)	146, 833, 1163, 1581, 1689, 1794
BlpI	GCTNAGC	1 cut(s)	1086
BlsI	GCNGC	6 cut(s)	147, 834, 1164, 1582, 1690, 1795
BmcAI	AGTACT	1 cut(s)	979
Bme1390I	CCNGG	4 cut(s)	198, 1059, 1508, 1877
Bme18I	GGWCC	5 cut(s)	68, 182, 194, 884, 1007
BmeRI	GACNNNNNGTC	1 cut(s)	192
BmeT110I	CYCGRG	1 cut(s)	912
BmgBI	CACGTC	1 cut(s)	1679
BmgT120I	GGNCC	8 cut(s)	68, 139, 140, 182, 194, 884, 1007, 1801
BmrFI	CCNGG	4 cut(s)	198, 1059, 1508, 1877
BmrI	ACTGGG	1 cut(s)	1531
BmsI	GCATC	1 cut(s)	1427
BmuI	ACTGGG	1 cut(s)	1531
Bpu10I	CCTNAGC	1 cut(s)	873
Bpu1102I	GCTNAGC	1 cut(s)	1086
BpuEI	CTTGAG	2 cut(s)	735, 1664
BpuMI	CCSGG	1 cut(s)	1877
Bsa29I	ATCGAT	1 cut(s)	19
BsaAI	YACGTR	2 cut(s)	1227, 1268
BsaHI	GRCGYC	2 cut(s)	30, 86
BsaI	GGTCTC	1 cut(s)	1915
BsaJI	CCNNGG	8 cut(s)	112, 256, 382, 863, 1232, 1492, 1876, 1925
BsaWI	WCCGGW	1 cut(s)	1829
BsaXI	ACNNNNNCTCC	8 cut(s)	20, 50, 265, 295, 337, 367, 388, 418
Bsc4I	CCNNNNNNNGG	6 cut(s)	95, 137, 191, 639, 972, 1279
Bse118I	RCCGGY	1 cut(s)	835
Bse1I	ACTGG	3 cut(s)	808, 1526, 1678
Bse3DI	GCAATG	3 cut(s)	591, 792, 1803
BseBI	CCWGG	3 cut(s)	198, 1059, 1508
BseCI	ATCGAT	1 cut(s)	19
BseDI	CCNNGG	8 cut(s)	112, 256, 382, 863, 1232, 1492, 1876, 1925
BseGI	GGATG	7 cut(s)	761, 1146, 1442, 1470, 1474, 1478, 1488
BseLI	CCNNNNNNNGG	6 cut(s)	95, 137, 191, 639, 972, 1279
BseMI	GCAATG	3 cut(s)	591, 792, 1803
BseMII	CTCAG	3 cut(s)	887, 1100, 1524
BseNI	ACTGG	3 cut(s)	808, 1526, 1678
BseRI	GAGGAG	5 cut(s)	474, 477, 504, 1872, 1875
BseSI	GKGCMC	1 cut(s)	143
BseX3I	CGGCCG	1 cut(s)	253
BseXI	GCAGC	4 cut(s)	819, 1567, 1700, 1780
BsgI	GTGCAG	1 cut(s)	1701
Bsh1236I	CGCG	2 cut(s)	1020, 1585
Bsh1285I	CGRYCG	1 cut(s)	256
BshNI	GGYRCC	4 cut(s)	85, 98, 213, 538
BshVI	ATCGAT	1 cut(s)	19
BsiEI	CGRYCG	1 cut(s)	256
BsiHKAI	GWGCWC	2 cut(s)	51, 1000
BsiHKCI	CYCGRG	1 cut(s)	912
BsiSI	CCGG	3 cut(s)	836, 1830, 1876
BsiWI	CGTACG	1 cut(s)	614
BslFI	GGGAC	1 cut(s)	1532
BslI	CCNNNNNNNGG	6 cut(s)	95, 137, 191, 639, 972, 1279
BsmAI	GTCTC	5 cut(s)	195, 486, 794, 1418, 1915
BsmBI	CGTCTC	1 cut(s)	1418
BsmFI	GGGAC	1 cut(s)	1532
Bso31I	GGTCTC	1 cut(s)	1915
BsoBI	CYCGRG	1 cut(s)	912
Bsp120I	GGGCCC	1 cut(s)	139
Bsp1286I	GDGCHC	4 cut(s)	51, 143, 1000, 1784
Bsp143I	GATC	9 cut(s)	1021, 1198, 1292, 1371, 1617, 1741, 1752, 1805, 1826
Bsp1720I	GCTNAGC	1 cut(s)	1086
Bsp19I	CCATGG	1 cut(s)	1925
BspCNI	CTCAG	3 cut(s)	886, 1099, 1525
BspDI	ATCGAT	1 cut(s)	19
BspFNI	CGCG	2 cut(s)	1020, 1585
BspMI	ACCTGC	1 cut(s)	1757
BspPI	GGATC	5 cut(s)	1300, 1747, 1749, 1821, 1834
BspT107I	GGYRCC	4 cut(s)	85, 98, 213, 538
BspTNI	GGTCTC	1 cut(s)	1915
BsrBI	CCGCTC	1 cut(s)	241
BsrDI	GCAATG	3 cut(s)	591, 792, 1803
BsrFI	RCCGGY	1 cut(s)	835
BsrI	ACTGG	3 cut(s)	808, 1526, 1678
BssAI	RCCGGY	1 cut(s)	835
BssECI	CCNNGG	8 cut(s)	112, 256, 382, 863, 1232, 1492, 1876, 1925
BssMI	GATC	9 cut(s)	1021, 1198, 1292, 1371, 1617, 1741, 1752, 1805, 1826
BssNI	GRCGYC	2 cut(s)	30, 86
BssT1I	CCWWGG	2 cut(s)	1232, 1925
Bst2UI	CCWGG	3 cut(s)	198, 1059, 1508
Bst4CI	ACNGT	4 cut(s)	44, 93, 1496, 1903
Bst6I	CTCTTC	2 cut(s)	451, 1099
BstACI	GRCGYC	2 cut(s)	30, 86
BstAPI	GCANNNNNTGC	2 cut(s)	1697, 1793
BstBAI	YACGTR	2 cut(s)	1227, 1268
BstC8I	GCNNGC	7 cut(s)	78, 143, 150, 837, 1300, 1479, 1719
BstDEI	CTNAG	3 cut(s)	873, 1086, 1533
BstDSI	CCRYGG	4 cut(s)	112, 382, 1492, 1925
BstF5I	GGATG	7 cut(s)	761, 1146, 1442, 1470, 1474, 1478, 1488
BstFNI	CGCG	2 cut(s)	1020, 1585
BstH2I	RGCGCY	3 cut(s)	77, 89, 842
BstHHI	GCGC	5 cut(s)	76, 88, 841, 857, 1585
BstKTI	GATC	9 cut(s)	1024, 1201, 1295, 1374, 1620, 1744, 1755, 1808, 1829
BstMAI	GTCTC	5 cut(s)	195, 486, 794, 1418, 1915
BstMBI	GATC	9 cut(s)	1021, 1198, 1292, 1371, 1617, 1741, 1752, 1805, 1826
BstMCI	CGRYCG	1 cut(s)	256
BstNI	CCWGG	3 cut(s)	198, 1059, 1508
BstSCI	CCNGG	4 cut(s)	196, 1057, 1506, 1875
BstSLI	GKGCMC	1 cut(s)	143
BstSNI	TACGTA	1 cut(s)	1268
BstUI	CGCG	2 cut(s)	1020, 1585
BstV1I	GCAGC	4 cut(s)	819, 1567, 1700, 1780
BstX2I	RGATCY	3 cut(s)	1741, 1752, 1826
BstXI	CCANNNNNNTGG	1 cut(s)	1576
BstYI	RGATCY	3 cut(s)	1741, 1752, 1826
BstZI	CGGCCG	1 cut(s)	253
Bsu15I	ATCGAT	1 cut(s)	19
BsuTUI	ATCGAT	1 cut(s)	19
BtgI	CCRYGG	4 cut(s)	112, 382, 1492, 1925
BtgZI	GCGATG	1 cut(s)	1406
BtrI	CACGTC	1 cut(s)	1679
BtsCI	GGATG	7 cut(s)	761, 1146, 1442, 1470, 1474, 1478, 1488
BtsI	GCAGTG	1 cut(s)	61
BtsIMutI	CAGTG	2 cut(s)	61, 1569
BveI	ACCTGC	1 cut(s)	1757
Cac8I	GCNNGC	7 cut(s)	78, 143, 150, 837, 1300, 1479, 1719
CaiI	CAGNNNCTG	2 cut(s)	77, 1697
CfoI	GCGC	5 cut(s)	76, 88, 841, 857, 1585
Cfr10I	RCCGGY	1 cut(s)	835
Cfr13I	GGNCC	8 cut(s)	68, 139, 140, 182, 194, 884, 1007, 1801
ClaI	ATCGAT	1 cut(s)	19
CseI	GACGC	1 cut(s)	279
Csp6I	GTAC	6 cut(s)	4, 214, 539, 615, 845, 978
CviAII	CATG	7 cut(s)	302, 473, 596, 644, 1150, 1882, 1926
CviQI	GTAC	6 cut(s)	4, 214, 539, 615, 845, 978
DdeI	CTNAG	3 cut(s)	873, 1086, 1533
DinI	GGCGCC	1 cut(s)	87
DpnI	GATC	9 cut(s)	1023, 1200, 1294, 1373, 1619, 1743, 1754, 1807, 1828
DpnII	GATC	9 cut(s)	1021, 1198, 1292, 1371, 1617, 1741, 1752, 1805, 1826
DraI	TTTAAA	2 cut(s)	948, 1540
DriI	GACNNNNNGTC	1 cut(s)	192
EaeI	YGGCCR	6 cut(s)	109, 253, 758, 1060, 1229, 1475
EagI	CGGCCG	1 cut(s)	253
Eam1104I	CTCTTC	2 cut(s)	451, 1099
Eam1105I	GACNNNNNGTC	1 cut(s)	192
EarI	CTCTTC	2 cut(s)	451, 1099
EclXI	CGGCCG	1 cut(s)	253
Eco105I	TACGTA	1 cut(s)	1268
Eco130I	CCWWGG	2 cut(s)	1232, 1925
Eco24I	GRGCYC	2 cut(s)	143, 1784
Eco31I	GGTCTC	1 cut(s)	1915
Eco32I	GATATC	1 cut(s)	649
Eco47I	GGWCC	5 cut(s)	68, 182, 194, 884, 1007
Eco52I	CGGCCG	1 cut(s)	253
Eco72I	CACGTG	1 cut(s)	1227
Eco88I	CYCGRG	1 cut(s)	912
EcoO109I	RGGNCCY	3 cut(s)	139, 884, 1007
EcoRI	GAATTC	1 cut(s)	1407
EcoRII	CCWGG	3 cut(s)	196, 1057, 1506
EcoRV	GATATC	1 cut(s)	649
EcoT14I	CCWWGG	2 cut(s)	1232, 1925
EcoT38I	GRGCYC	2 cut(s)	143, 1784
EgeI	GGCGCC	1 cut(s)	87
EheI	GGCGCC	1 cut(s)	87
ErhI	CCWWGG	2 cut(s)	1232, 1925
Esp3I	CGTCTC	1 cut(s)	1418
FaeI	CATG	7 cut(s)	305, 476, 599, 647, 1153, 1885, 1929
FalI	AAGNNNNNCTT	2 cut(s)	1096, 1128
FaqI	GGGAC	1 cut(s)	1532
FatI	CATG	7 cut(s)	301, 472, 595, 643, 1149, 1881, 1925
FauI	CCCGC	4 cut(s)	150, 225, 418, 615
FbaI	TGATCA	2 cut(s)	1371, 1617
Fnu4HI	GCNGC	6 cut(s)	146, 833, 1163, 1581, 1689, 1794
FokI	GGATG	7 cut(s)	768, 1153, 1449, 1475, 1477, 1481, 1485
FriOI	GRGCYC	2 cut(s)	143, 1784
Fsp4HI	GCNGC	6 cut(s)	146, 833, 1163, 1581, 1689, 1794
FspBI	CTAG	4 cut(s)	62, 701, 1118, 1737
GlaI	GCGC	5 cut(s)	75, 87, 840, 856, 1584
GluI	GCNGC	6 cut(s)	146, 833, 1163, 1581, 1689, 1794
HaeII	RGCGCY	3 cut(s)	77, 89, 842
HapII	CCGG	3 cut(s)	836, 1830, 1876
HgaI	GACGC	1 cut(s)	279
HhaI	GCGC	5 cut(s)	76, 88, 841, 857, 1585
Hin1I	GRCGYC	2 cut(s)	30, 86
Hin1II	CATG	7 cut(s)	305, 476, 599, 647, 1153, 1885, 1929
Hin6I	GCGC	5 cut(s)	74, 86, 839, 855, 1583
HinP1I	GCGC	5 cut(s)	74, 86, 839, 855, 1583
HincII	GTYRAC	1 cut(s)	34
HindII	GTYRAC	1 cut(s)	34
HindIII	AAGCTT	1 cut(s)	942
HinfI	GANTC	3 cut(s)	661, 1552, 1662
HpaII	CCGG	3 cut(s)	836, 1830, 1876
HphI	GGTGA	2 cut(s)	718, 1508
Hpy166II	GTNNAC	4 cut(s)	34, 182, 959, 1657
Hpy188I	TCNGA	6 cut(s)	13, 592, 666, 1413, 1667, 1823
Hpy188III	TCNNGA	6 cut(s)	486, 914, 935, 1592, 1750, 1760
Hpy8I	GTNNAC	4 cut(s)	34, 182, 959, 1657
Hpy99I	CGWCG	2 cut(s)	32, 295
HpyAV	CCTTC	6 cut(s)	309, 582, 632, 875, 1594, 1766
HpyCH4III	ACNGT	4 cut(s)	44, 93, 1496, 1903
HpyCH4IV	ACGT	6 cut(s)	30, 961, 1226, 1267, 1446, 1678
HpyCH4V	TGCA	5 cut(s)	984, 1682, 1727, 1787, 1796
HpyF3I	CTNAG	3 cut(s)	873, 1086, 1533
HpySE526I	ACGT	6 cut(s)	30, 961, 1226, 1267, 1446, 1678
Hsp92I	GRCGYC	2 cut(s)	30, 86
Hsp92II	CATG	7 cut(s)	305, 476, 599, 647, 1153, 1885, 1929
HspAI	GCGC	5 cut(s)	74, 86, 839, 855, 1583
KasI	GGCGCC	1 cut(s)	85
KpnI	GGTACC	2 cut(s)	217, 542
KroI	GCCGGC	1 cut(s)	835
KroNI	GCCGGC	1 cut(s)	837
Ksp22I	TGATCA	2 cut(s)	1371, 1617
Kzo9I	GATC	9 cut(s)	1021, 1198, 1292, 1371, 1617, 1741, 1752, 1805, 1826
LmnI	GCTCC	6 cut(s)	133, 273, 396, 1003, 1401, 1685
Lsp1109I	GCAGC	4 cut(s)	819, 1567, 1700, 1780
LweI	GCATC	1 cut(s)	1427
MaeI	CTAG	4 cut(s)	62, 701, 1118, 1737
MaeII	ACGT	6 cut(s)	30, 961, 1226, 1267, 1446, 1678
MaeIII	GTNAC	6 cut(s)	56, 1108, 1268, 1342, 1447, 1913
MalI	GATC	9 cut(s)	1023, 1200, 1294, 1373, 1619, 1743, 1754, 1807, 1828
MbiI	CCGCTC	1 cut(s)	241
MboI	GATC	9 cut(s)	1021, 1198, 1292, 1371, 1617, 1741, 1752, 1805, 1826
MboII	GAAGA	4 cut(s)	468, 562, 950, 1116
MfeI	CAATTG	1 cut(s)	1457
MflI	RGATCY	3 cut(s)	1741, 1752, 1826
MhlI	GDGCHC	4 cut(s)	51, 143, 1000, 1784
MlsI	TGGCCA	3 cut(s)	760, 1231, 1477
MluNI	TGGCCA	3 cut(s)	760, 1231, 1477
Mly113I	GGCGCC	1 cut(s)	86
MmeI	TCCRAC	3 cut(s)	36, 1059, 1125
Mox20I	TGGCCA	3 cut(s)	760, 1231, 1477
MroNI	GCCGGC	1 cut(s)	835
MroXI	GAANNNNTTC	1 cut(s)	554
MscI	TGGCCA	3 cut(s)	760, 1231, 1477
MseI	TTAA	8 cut(s)	207, 770, 785, 947, 1026, 1305, 1539, 1703
MslI	CAYNNNNRTG	4 cut(s)	54, 306, 1574, 1722
Msp20I	TGGCCA	3 cut(s)	760, 1231, 1477
MspI	CCGG	3 cut(s)	836, 1830, 1876
MspR9I	CCNGG	4 cut(s)	198, 1059, 1508, 1877
MunI	CAATTG	1 cut(s)	1457
MvaI	CCWGG	3 cut(s)	198, 1059, 1508
MvnI	CGCG	2 cut(s)	1020, 1585
NaeI	GCCGGC	1 cut(s)	837
NarI	GGCGCC	1 cut(s)	86
NciI	CCSGG	1 cut(s)	1877
NcoI	CCATGG	1 cut(s)	1925
NdeII	GATC	9 cut(s)	1021, 1198, 1292, 1371, 1617, 1741, 1752, 1805, 1826
NgoMIV	GCCGGC	1 cut(s)	835
NlaIII	CATG	7 cut(s)	305, 476, 599, 647, 1153, 1885, 1929
NmeAIII	GCCGAG	2 cut(s)	281, 823
NmuCI	GTSAC	3 cut(s)	56, 1108, 1342
OliI	CACNNNNGTG	2 cut(s)	54, 1574
PaeR7I	CTCGAG	1 cut(s)	912
PcsI	WCGNNNNNNNCGW	1 cut(s)	1675
PdiI	GCCGGC	1 cut(s)	837
PdmI	GAANNNNTTC	1 cut(s)	554
PfeI	GAWTC	3 cut(s)	661, 1552, 1662
Pfl23II	CGTACG	1 cut(s)	614
PflFI	GACNNNGTC	2 cut(s)	820, 1523
PfoI	TCCNGGA	1 cut(s)	196
PkrI	GCNGC	6 cut(s)	147, 834, 1164, 1582, 1690, 1795
PluTI	GGCGCC	1 cut(s)	89
PmaCI	CACGTG	1 cut(s)	1227
PmlI	CACGTG	1 cut(s)	1227
Ppu21I	YACGTR	2 cut(s)	1227, 1268
PpuMI	RGGWCCY	2 cut(s)	884, 1007
Psp5II	RGGWCCY	2 cut(s)	884, 1007
Psp6I	CCWGG	3 cut(s)	196, 1057, 1506
PspCI	CACGTG	1 cut(s)	1227
PspGI	CCWGG	3 cut(s)	196, 1057, 1506
PspLI	CGTACG	1 cut(s)	614
PspOMI	GGGCCC	1 cut(s)	139
PspPI	GGNCC	8 cut(s)	68, 139, 140, 182, 194, 884, 1007, 1801
PspPPI	RGGWCCY	2 cut(s)	884, 1007
PsrI	GAACNNNNNNTAC	2 cut(s)	206, 238
PstNI	CAGNNNCTG	2 cut(s)	77, 1697
PsuI	RGATCY	3 cut(s)	1741, 1752, 1826
PsyI	GACNNNGTC	2 cut(s)	820, 1523
RsaI	GTAC	6 cut(s)	5, 215, 540, 616, 846, 979
RsaNI	GTAC	6 cut(s)	4, 214, 539, 615, 845, 978
RseI	CAYNNNNRTG	4 cut(s)	54, 306, 1574, 1722
SaqAI	TTAA	8 cut(s)	207, 770, 785, 947, 1026, 1305, 1539, 1703
SatI	GCNGC	6 cut(s)	146, 833, 1163, 1581, 1689, 1794
Sau3AI	GATC	9 cut(s)	1021, 1198, 1292, 1371, 1617, 1741, 1752, 1805, 1826
Sau96I	GGNCC	8 cut(s)	68, 139, 140, 182, 194, 884, 1007, 1801
ScaI	AGTACT	1 cut(s)	979
ScrFI	CCNGG	4 cut(s)	198, 1059, 1508, 1877
SduI	GDGCHC	4 cut(s)	51, 143, 1000, 1784
SfaNI	GCATC	1 cut(s)	1427
SfoI	GGCGCC	1 cut(s)	87
Sfr274I	CTCGAG	1 cut(s)	912
SinI	GGWCC	5 cut(s)	68, 182, 194, 884, 1007
SlaI	CTCGAG	1 cut(s)	912
SmiMI	CAYNNNNRTG	4 cut(s)	54, 306, 1574, 1722
SmlI	CTYRAG	3 cut(s)	750, 912, 1643
SmoI	CTYRAG	3 cut(s)	750, 912, 1643
SnaBI	TACGTA	1 cut(s)	1268
SspDI	GGCGCC	1 cut(s)	85
SspI	AATATT	2 cut(s)	505, 970
SspMI	CTAG	4 cut(s)	62, 701, 1118, 1737
StyD4I	CCNGG	4 cut(s)	196, 1057, 1506, 1875
StyI	CCWWGG	2 cut(s)	1232, 1925
TaaI	ACNGT	4 cut(s)	44, 93, 1496, 1903
TaiI	ACGT	6 cut(s)	33, 964, 1229, 1270, 1449, 1681
TaqI	TCGA	4 cut(s)	19, 237, 293, 913
TaqII	GACCGA	1 cut(s)	199
TatI	WGTACW	1 cut(s)	977
TauI	GCSGC	2 cut(s)	148, 1165
TfiI	GAWTC	3 cut(s)	661, 1552, 1662
Tru1I	TTAA	8 cut(s)	207, 770, 785, 947, 1026, 1305, 1539, 1703
Tru9I	TTAA	8 cut(s)	207, 770, 785, 947, 1026, 1305, 1539, 1703
TscAI	CASTG	2 cut(s)	61, 1576
TseFI	GTSAC	3 cut(s)	56, 1108, 1342
TseI	GCWGC	4 cut(s)	832, 1580, 1688, 1793
Tsp45I	GTSAC	3 cut(s)	56, 1108, 1342
TspDTI	ATGAA	1 cut(s)	150
TspGWI	ACGGA	3 cut(s)	120, 695, 1361
TspRI	CASTG	2 cut(s)	61, 1576
Tth111I	GACNNNGTC	2 cut(s)	820, 1523
VpaK11BI	GGWCC	5 cut(s)	68, 182, 194, 884, 1007
XapI	RAATTY	5 cut(s)	930, 950, 1096, 1407, 1730
XhoI	CTCGAG	1 cut(s)	912
XmnI	GAANNNNTTC	1 cut(s)	554
XspI	CTAG	4 cut(s)	62, 701, 1118, 1737
ZraI	GACGTC	1 cut(s)	31
ZrmI	AGTACT	1 cut(s)	979

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.