Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0005996

Physical Location & Seq

Genomic Coordinates Forward (+)

26622478 .. 26625665

Gene Structure 3188 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1172300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1596 bp

ATGTACGCCTACGACAACCGACACGCCGACGTCAACTCCTACCGTGAGCGCCGAAGTGACCTAATGGGTCCAGCGCCTGCGGCTGGTGGCACCGCTTATGGCCGTGGAGGTGGGCCCGCTCCGTATGGGGGCCCATCGCCTCCGGTTGCTCCGTATTCGGCAAGGGTGGGTGGACCGGCACAGGGTCCAGGAGACTTTAATGGGTACCCGCCGTTCCAGCCCCCTTCCGAGCGGTTCAATATCGGTCGAGGTGGTGGTAATGGGAGCTTTGGTGGAAGAGCGTCGAATGGACATGTTGGTGGTGGAAGGGGTCGTGGTGGTAGTGTTGGGAGATTTGGTGGCGGTGGCAGGGGTTTTGATGGAGGCCGTGGTGGCGGTAGGAGCTTCGGTGGTAGTCGTGGTGGAGGTGCGGGTTTCGGCGGTGGGCGTGGAGTTGGTGGAAGAGGAGGTAGGCATGGTGGCTCGTCAAGAGGAGACTTGGATAATATTATGCTTCCCAAGCAAGATTTTGGGACCTTGGTACCTTTTGAAAAGAATTTCTATGCTGAAAGTCCTTCAGTGAGGGCAATGTCAGAGCATGATGTTATGGTGTATCGTACTAGGCGGGAGATTACTGTTGAAGGGCATGACATCCCAAAGCCGATTCAGATGTTTGAGGATGCGAATTTCCCTGATTACTGCCTCGAGGCGATTGCAAAATTGGGTTTTGTTGAACCAACACCAATTCAGGCTCAAGGATGGCCAATGGCATTAAAGGGTAGAGATTTAATTGGCATCGCTGAGACTGGTTCTGGTAAGACTTTGTCGTATCTGCTGCCTGCTTTGGTACACATTAGTGCGCAGCCTCGGTTAGCTCAGGGAGAAGGTCCTATTGCATTAGTATTAGCACCTACTCGAGAATTGGCAGTTCAAATTCAAGAAGAAGCTTTAAAATTTGGCTTACGTTCCAATATTAGGAGTACTTGCATTTATGGCGGTGCTCCAAAAGGACCCCAAATACGCGACCTTAAAAGAGGGGTTGAGATTGTTATAGCTACGCCTGGCCGACTGATAGATATGTTGGAAGCTCAGCATACAAATTTGCGAAGAGTCACTTATCTAGTTTTAGATGAGGCTGATAGGATGTTGGACATGGGATTTGAGCCACAGATAAGGAAAATTGTTTCCCAAACCCGACCAGATCGGCAGACATTGTATTGGAGTGCCACGTGGCCAAGGGAGGTTGAAAGTTTAGCAAGGCAGTTTTTACGTAACCCATATAAGGTAATCATTGGATCAGCAAGCCTTAAAGCAAACCAATCCATAAACCAAGTTGTTGAAGTTGTGACGGAGGTGGAGAAATACAATAGGCTGATCAAATTGCTCAAAGAAGCAATGGTTGGGAGCAGAATTCTGATATTTGTGGAGACGAAAAAGGGATGTGACTACGTTACTAAACAATTGAGGATGGATGGATGGCCAGCTCTATCCATCCATGGTGATAAAAACCAGACTGAAAGGGACTGGGTCTTGGCTGAGTTTAAAAGTGGCAGGAACCCAATAATGACTGCCACCGATGTGGCTGCGCGGGGTCTTGGTAGGATCATTGTGTGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003729 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005829 GO:0006139 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010501 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko03040 ko05202 ko05205 map03040 map05202 map05205

Pfam Domains

Protein Families

DEAD Helicase_C

Protein Analysis

532

Amino Acids

57.21

Weight (kDa)

9.65

Isoelectric Point (pI)

41.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	240 - 410	6.7e-49	DEAD/DEAH box helicase
Helicase_C	PF00271	446 - 525	1.8e-14	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005996)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G63120 AT5G63120 AT5G63120 AT5G63120 AT5G63120
fragaria_vesca	FvH4_5g06110 FvH4_5g06110 FvH4_5g06110 FvH4_5g06110 FvH4_5g06110 FvH4_5g06110
malus_domestica	MD06G1166600.v1.1 MD14G1172300.v1.1
prunus_persica	Prupe.5G166600_v2.0.a1 Prupe.5G166600_v2.0.a1 Prupe.5G166600_v2.0.a1
pyrus_communis	pycom06g14930 pycom14g14390
rosa_chinensis	RchiOBHm_Chr7g0192711
rosa_laevigata	RLG00000004335
rosa_multiflora	Rmu_sc0002040.1_g000012
rosa_roxburghii	Rroxscaffold_3G00262730
rosa_rugosa	Rorug07G0004000 Rorug07G0004100
rosa_samantha	Rh7CG133900 Rh7DG131300
rosa_wichuraiana	Rw7G011040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	33
AbsI	CCTCGAGG	1 cut(s)	683
Acc16I	TGCGCA	1 cut(s)	840
Acc65I	GGTACC	2 cut(s)	204, 520
AccB1I	GGYRCC	3 cut(s)	89, 204, 520
AccBSI	CCGCTC	2 cut(s)	119, 232
AccII	CGCG	2 cut(s)	1002, 1567
AclWI	GGATC	2 cut(s)	1282, 1589
AcoI	YGGCCR	5 cut(s)	100, 740, 1042, 1211, 1457
AcsI	RAATTY	6 cut(s)	535, 664, 912, 932, 1078, 1389
AcuI	CTGAAG	1 cut(s)	540
AcvI	CACGTG	1 cut(s)	1209
AcyI	GRCGYC	1 cut(s)	30
AfaI	GTAC	6 cut(s)	5, 206, 522, 598, 828, 961
AfiI	CCNNNNNNNGG	5 cut(s)	83, 128, 182, 954, 1261
AflIII	ACRYGT	1 cut(s)	292
AgsI	TTSAA	8 cut(s)	238, 530, 620, 713, 911, 917, 1226, 1319
AjnI	CCWGG	2 cut(s)	187, 1039
AjuI	GAANNNNNNNTTGG	2 cut(s)	1362, 1394
AleI	CACNNNNGTG	2 cut(s)	834, 1556
AluBI	AGCT	7 cut(s)	267, 384, 854, 926, 1034, 1067, 1463
AluI	AGCT	7 cut(s)	267, 384, 854, 926, 1034, 1067, 1463
Alw21I	GWGCWC	1 cut(s)	982
Alw26I	GTCTC	4 cut(s)	186, 468, 776, 1400
AlwI	GGATC	2 cut(s)	1282, 1589
AlwNI	CAGNNNCTG	1 cut(s)	77
Ama87I	CYCGRG	2 cut(s)	683, 894
AoxI	GGCC	8 cut(s)	100, 113, 130, 364, 740, 1042, 1211, 1457
ApaI	GGGCCC	2 cut(s)	117, 134
ApeKI	GCWGC	3 cut(s)	814, 841, 1562
ApoI	RAATTY	6 cut(s)	535, 664, 912, 932, 1078, 1389
ArsI	GACNNNNNNTTYG	2 cut(s)	1121, 1153
Asp718I	GGTACC	2 cut(s)	204, 520
AspLEI	GCGC	4 cut(s)	51, 76, 841, 1567
AsuHPI	GGTGA	1 cut(s)	1490
AvaI	CYCGRG	2 cut(s)	683, 894
AvaII	GGWCC	6 cut(s)	68, 173, 185, 513, 866, 989
BaeGI	GKGCMC	2 cut(s)	117, 134
BaeI	ACNNNNGTAYC	2 cut(s)	504, 537
BalI	TGGCCA	3 cut(s)	742, 1213, 1459
BanI	GGYRCC	3 cut(s)	89, 204, 520
BanII	GRGCYC	2 cut(s)	117, 134
BbrPI	CACGTG	1 cut(s)	1209
Bbv12I	GWGCWC	1 cut(s)	982
BbvI	GCAGC	3 cut(s)	801, 853, 1549
BccI	CCATC	7 cut(s)	142, 353, 732, 1441, 1445, 1449, 1478
BceAI	ACGGC	3 cut(s)	87, 196, 351
BcgI	CGANNNNNNTGC	4 cut(s)	674, 708, 1544, 1578
BciT130I	CCWGG	2 cut(s)	189, 1041
BclI	TGATCA	1 cut(s)	1353
BcoDI	GTCTC	4 cut(s)	186, 468, 776, 1400
BfaI	CTAG	3 cut(s)	600, 1100, 1594
BfoI	RGCGCY	2 cut(s)	52, 77
BglI	GCCNNNNNGGC	1 cut(s)	372
BisI	GCNGC	4 cut(s)	81, 815, 842, 1563
BlpI	GCTNAGC	1 cut(s)	1068
BlsI	GCNGC	4 cut(s)	82, 816, 843, 1564
BmcAI	AGTACT	1 cut(s)	961
Bme1390I	CCNGG	2 cut(s)	189, 1041
Bme18I	GGWCC	6 cut(s)	68, 173, 185, 513, 866, 989
BmeT110I	CYCGRG	2 cut(s)	683, 894
BmrFI	CCNGG	2 cut(s)	189, 1041
BmrI	ACTGGG	1 cut(s)	1513
BmsI	GCATC	2 cut(s)	649, 783
BmuI	ACTGGG	1 cut(s)	1513
Bpu10I	CCTNAGC	1 cut(s)	855
Bpu1102I	GCTNAGC	1 cut(s)	1068
BpuEI	CTTGAG	1 cut(s)	717
BsaAI	YACGTR	2 cut(s)	1209, 1250
BsaHI	GRCGYC	1 cut(s)	30
BsaJI	CCNNGG	6 cut(s)	103, 367, 516, 845, 1214, 1474
BsaWI	WCCGGW	1 cut(s)	142
BsaXI	ACNNNNNCTCC	8 cut(s)	20, 50, 256, 286, 322, 352, 373, 403
Bsc4I	CCNNNNNNNGG	5 cut(s)	83, 128, 182, 954, 1261
Bse118I	RCCGGY	1 cut(s)	175
Bse1I	ACTGG	2 cut(s)	790, 1508
Bse3DI	GCAATG	2 cut(s)	573, 1380
BseBI	CCWGG	2 cut(s)	189, 1041
BseDI	CCNNGG	6 cut(s)	103, 367, 516, 845, 1214, 1474
BseGI	GGATG	9 cut(s)	630, 664, 743, 1128, 1424, 1452, 1456, 1460, 1470
BseLI	CCNNNNNNNGG	5 cut(s)	83, 128, 182, 954, 1261
BseMI	GCAATG	2 cut(s)	573, 1380
BseMII	CTCAG	4 cut(s)	771, 869, 1082, 1506
BseNI	ACTGG	2 cut(s)	790, 1508
BseRI	GAGGAG	2 cut(s)	459, 486
BseSI	GKGCMC	2 cut(s)	117, 134
BseXI	GCAGC	3 cut(s)	801, 853, 1549
Bsh1236I	CGCG	2 cut(s)	1002, 1567
Bsh1285I	CGRYCG	1 cut(s)	247
BshFI	GGCC	8 cut(s)	102, 115, 132, 366, 742, 1044, 1213, 1459
BshNI	GGYRCC	3 cut(s)	89, 204, 520
BsiEI	CGRYCG	1 cut(s)	247
BsiHKAI	GWGCWC	1 cut(s)	982
BsiHKCI	CYCGRG	2 cut(s)	683, 894
BsiSI	CCGG	2 cut(s)	143, 176
BslFI	GGGAC	2 cut(s)	526, 1514
BslI	CCNNNNNNNGG	5 cut(s)	83, 128, 182, 954, 1261
BsmAI	GTCTC	4 cut(s)	186, 468, 776, 1400
BsmBI	CGTCTC	1 cut(s)	1400
BsmFI	GGGAC	2 cut(s)	526, 1514
BsnI	GGCC	8 cut(s)	102, 115, 132, 366, 742, 1044, 1213, 1459
BsoBI	CYCGRG	2 cut(s)	683, 894
Bsp120I	GGGCCC	2 cut(s)	113, 130
Bsp1286I	GDGCHC	3 cut(s)	117, 134, 982
Bsp143I	GATC	4 cut(s)	1180, 1274, 1353, 1581
Bsp1720I	GCTNAGC	1 cut(s)	1068
Bsp19I	CCATGG	1 cut(s)	1474
BspANI	GGCC	8 cut(s)	102, 115, 132, 366, 742, 1044, 1213, 1459
BspCNI	CTCAG	4 cut(s)	772, 868, 1081, 1507
BspFNI	CGCG	2 cut(s)	1002, 1567
BspPI	GGATC	2 cut(s)	1282, 1589
BspQI	GCTCTTC	1 cut(s)	271
BspT107I	GGYRCC	3 cut(s)	89, 204, 520
BsrBI	CCGCTC	2 cut(s)	119, 232
BsrDI	GCAATG	2 cut(s)	573, 1380
BsrFI	RCCGGY	1 cut(s)	175
BsrI	ACTGG	2 cut(s)	790, 1508
BssAI	RCCGGY	1 cut(s)	175
BssECI	CCNNGG	6 cut(s)	103, 367, 516, 845, 1214, 1474
BssMI	GATC	4 cut(s)	1180, 1274, 1353, 1581
BssNI	GRCGYC	1 cut(s)	30
BssT1I	CCWWGG	3 cut(s)	516, 1214, 1474
Bst2UI	CCWGG	2 cut(s)	189, 1041
Bst4CI	ACNGT	2 cut(s)	44, 616
Bst6I	CTCTTC	3 cut(s)	271, 436, 1081
BstACI	GRCGYC	1 cut(s)	30
BstBAI	YACGTR	2 cut(s)	1209, 1250
BstC8I	GCNNGC	5 cut(s)	78, 117, 819, 1282, 1461
BstDEI	CTNAG	4 cut(s)	780, 855, 1068, 1515
BstDSI	CCRYGG	3 cut(s)	103, 367, 1474
BstF5I	GGATG	9 cut(s)	630, 664, 743, 1128, 1424, 1452, 1456, 1460, 1470
BstFNI	CGCG	2 cut(s)	1002, 1567
BstH2I	RGCGCY	2 cut(s)	52, 77
BstHHI	GCGC	4 cut(s)	51, 76, 841, 1567
BstKTI	GATC	4 cut(s)	1183, 1277, 1356, 1584
BstMAI	GTCTC	4 cut(s)	186, 468, 776, 1400
BstMBI	GATC	4 cut(s)	1180, 1274, 1353, 1581
BstMCI	CGRYCG	1 cut(s)	247
BstMWI	GCNNNNNNNGC	6 cut(s)	80, 217, 372, 381, 499, 972
BstNI	CCWGG	2 cut(s)	189, 1041
BstNSI	RCATGY	1 cut(s)	296
BstSCI	CCNGG	2 cut(s)	187, 1039
BstSLI	GKGCMC	2 cut(s)	117, 134
BstSNI	TACGTA	1 cut(s)	1250
BstUI	CGCG	2 cut(s)	1002, 1567
BstV1I	GCAGC	3 cut(s)	801, 853, 1549
BstXI	CCANNNNNNTGG	1 cut(s)	1558
BsuRI	GGCC	8 cut(s)	102, 115, 132, 366, 742, 1044, 1213, 1459
BtgI	CCRYGG	3 cut(s)	103, 367, 1474
BtgZI	GCGATG	2 cut(s)	120, 760
BtsCI	GGATG	9 cut(s)	630, 664, 743, 1128, 1424, 1452, 1456, 1460, 1470
BtsIMutI	CAGTG	1 cut(s)	564
Cac8I	GCNNGC	5 cut(s)	78, 117, 819, 1282, 1461
CaiI	CAGNNNCTG	1 cut(s)	77
CfoI	GCGC	4 cut(s)	51, 76, 841, 1567
Cfr10I	RCCGGY	1 cut(s)	175
CseI	GACGC	1 cut(s)	270
Csp6I	GTAC	6 cut(s)	4, 205, 521, 597, 827, 960
CviAII	CATG	6 cut(s)	293, 455, 578, 626, 1132, 1475
CviQI	GTAC	6 cut(s)	4, 205, 521, 597, 827, 960
DdeI	CTNAG	4 cut(s)	780, 855, 1068, 1515
DpnI	GATC	4 cut(s)	1182, 1276, 1355, 1583
DpnII	GATC	4 cut(s)	1180, 1274, 1353, 1581
DraI	TTTAAA	2 cut(s)	930, 1522
EaeI	YGGCCR	5 cut(s)	100, 740, 1042, 1211, 1457
Eam1104I	CTCTTC	3 cut(s)	271, 436, 1081
EarI	CTCTTC	3 cut(s)	271, 436, 1081
Eco105I	TACGTA	1 cut(s)	1250
Eco130I	CCWWGG	3 cut(s)	516, 1214, 1474
Eco24I	GRGCYC	2 cut(s)	117, 134
Eco47I	GGWCC	6 cut(s)	68, 173, 185, 513, 866, 989
Eco57I	CTGAAG	1 cut(s)	540
Eco72I	CACGTG	1 cut(s)	1209
Eco88I	CYCGRG	2 cut(s)	683, 894
EcoO109I	RGGNCCY	4 cut(s)	130, 513, 866, 989
EcoRI	GAATTC	1 cut(s)	1389
EcoRII	CCWGG	2 cut(s)	187, 1039
EcoT14I	CCWWGG	3 cut(s)	516, 1214, 1474
EcoT38I	GRGCYC	2 cut(s)	117, 134
ErhI	CCWWGG	3 cut(s)	516, 1214, 1474
Esp3I	CGTCTC	1 cut(s)	1400
FaeI	CATG	6 cut(s)	296, 458, 581, 629, 1135, 1478
FalI	AAGNNNNNCTT	2 cut(s)	1078, 1110
FaqI	GGGAC	2 cut(s)	526, 1514
FatI	CATG	6 cut(s)	292, 454, 577, 625, 1131, 1474
FauI	CCCGC	5 cut(s)	124, 216, 403, 597, 1560
FbaI	TGATCA	1 cut(s)	1353
Fnu4HI	GCNGC	4 cut(s)	81, 815, 842, 1563
FokI	GGATG	9 cut(s)	617, 671, 750, 1135, 1431, 1457, 1459, 1463, 1467
FriOI	GRGCYC	2 cut(s)	117, 134
Fsp4HI	GCNGC	4 cut(s)	81, 815, 842, 1563
FspBI	CTAG	3 cut(s)	600, 1100, 1594
FspI	TGCGCA	1 cut(s)	840
GlaI	GCGC	4 cut(s)	50, 75, 840, 1566
GluI	GCNGC	4 cut(s)	81, 815, 842, 1563
HaeII	RGCGCY	2 cut(s)	52, 77
HaeIII	GGCC	8 cut(s)	102, 115, 132, 366, 742, 1044, 1213, 1459
HapII	CCGG	2 cut(s)	143, 176
HgaI	GACGC	1 cut(s)	270
HhaI	GCGC	4 cut(s)	51, 76, 841, 1567
Hin1I	GRCGYC	1 cut(s)	30
Hin1II	CATG	6 cut(s)	296, 458, 581, 629, 1135, 1478
Hin6I	GCGC	4 cut(s)	49, 74, 839, 1565
HinP1I	GCGC	4 cut(s)	49, 74, 839, 1565
HincII	GTYRAC	1 cut(s)	34
HindII	GTYRAC	1 cut(s)	34
HindIII	AAGCTT	1 cut(s)	924
HinfI	GANTC	2 cut(s)	643, 1089
HpaII	CCGG	2 cut(s)	143, 176
HphI	GGTGA	1 cut(s)	1490
Hpy166II	GTNNAC	3 cut(s)	34, 173, 829
Hpy188I	TCNGA	4 cut(s)	229, 574, 648, 1395
Hpy188III	TCNNGA	3 cut(s)	468, 896, 917
Hpy8I	GTNNAC	3 cut(s)	34, 173, 829
Hpy99I	CGWCG	2 cut(s)	32, 286
HpyAV	CCTTC	5 cut(s)	234, 300, 564, 614, 857
HpyCH4III	ACNGT	2 cut(s)	44, 616
HpyCH4IV	ACGT	5 cut(s)	30, 943, 1208, 1249, 1428
HpyCH4V	TGCA	3 cut(s)	695, 875, 966
HpyF10VI	GCNNNNNNNGC	6 cut(s)	80, 217, 372, 381, 499, 972
HpyF3I	CTNAG	4 cut(s)	780, 855, 1068, 1515
HpySE526I	ACGT	5 cut(s)	30, 943, 1208, 1249, 1428
Hsp92I	GRCGYC	1 cut(s)	30
Hsp92II	CATG	6 cut(s)	296, 458, 581, 629, 1135, 1478
HspAI	GCGC	4 cut(s)	49, 74, 839, 1565
KpnI	GGTACC	2 cut(s)	208, 524
Ksp22I	TGATCA	1 cut(s)	1353
Kzo9I	GATC	4 cut(s)	1180, 1274, 1353, 1581
LguI	GCTCTTC	1 cut(s)	271
LmnI	GCTCC	6 cut(s)	124, 154, 264, 381, 985, 1383
Lsp1109I	GCAGC	3 cut(s)	801, 853, 1549
LweI	GCATC	2 cut(s)	649, 783
MaeI	CTAG	3 cut(s)	600, 1100, 1594
MaeII	ACGT	5 cut(s)	30, 943, 1208, 1249, 1428
MaeIII	GTNAC	6 cut(s)	56, 1090, 1250, 1324, 1421, 1429
MalI	GATC	4 cut(s)	1182, 1276, 1355, 1583
MbiI	CCGCTC	2 cut(s)	119, 232
MboI	GATC	4 cut(s)	1180, 1274, 1353, 1581
MboII	GAAGA	4 cut(s)	288, 453, 932, 1098
MfeI	CAATTG	1 cut(s)	1439
MhlI	GDGCHC	3 cut(s)	117, 134, 982
MlsI	TGGCCA	3 cut(s)	742, 1213, 1459
MluNI	TGGCCA	3 cut(s)	742, 1213, 1459
MlyI	GAGTC	1 cut(s)	1098
MmeI	TCCRAC	2 cut(s)	1041, 1107
Mox20I	TGGCCA	3 cut(s)	742, 1213, 1459
MscI	TGGCCA	3 cut(s)	742, 1213, 1459
MseI	TTAA	7 cut(s)	198, 752, 767, 929, 1008, 1287, 1521
MslI	CAYNNNNRTG	3 cut(s)	297, 834, 1556
Msp20I	TGGCCA	3 cut(s)	742, 1213, 1459
MspI	CCGG	2 cut(s)	143, 176
MspR9I	CCNGG	2 cut(s)	189, 1041
MunI	CAATTG	1 cut(s)	1439
MvaI	CCWGG	2 cut(s)	189, 1041
MvnI	CGCG	2 cut(s)	1002, 1567
MwoI	GCNNNNNNNGC	6 cut(s)	80, 217, 372, 381, 499, 972
NcoI	CCATGG	1 cut(s)	1474
NdeII	GATC	4 cut(s)	1180, 1274, 1353, 1581
NlaIII	CATG	6 cut(s)	296, 458, 581, 629, 1135, 1478
NmuCI	GTSAC	4 cut(s)	56, 1090, 1324, 1421
NsbI	TGCGCA	1 cut(s)	840
NspI	RCATGY	1 cut(s)	296
OliI	CACNNNNGTG	2 cut(s)	834, 1556
PaeR7I	CTCGAG	2 cut(s)	683, 894
PciI	ACATGT	1 cut(s)	292
PciSI	GCTCTTC	1 cut(s)	271
PfeI	GAWTC	1 cut(s)	643
PflFI	GACNNNGTC	2 cut(s)	802, 1505
PfoI	TCCNGGA	1 cut(s)	187
PkrI	GCNGC	4 cut(s)	82, 816, 843, 1564
PleI	GAGTC	1 cut(s)	1097
PmaCI	CACGTG	1 cut(s)	1209
PmlI	CACGTG	1 cut(s)	1209
PpsI	GAGTC	1 cut(s)	1097
Ppu21I	YACGTR	2 cut(s)	1209, 1250
PpuMI	RGGWCCY	3 cut(s)	513, 866, 989
PscI	ACATGT	1 cut(s)	292
Psp5II	RGGWCCY	3 cut(s)	513, 866, 989
Psp6I	CCWGG	2 cut(s)	187, 1039
PspCI	CACGTG	1 cut(s)	1209
PspGI	CCWGG	2 cut(s)	187, 1039
PspOMI	GGGCCC	2 cut(s)	113, 130
PspPPI	RGGWCCY	3 cut(s)	513, 866, 989
PspXI	VCTCGAGB	1 cut(s)	683
PsrI	GAACNNNNNNTAC	2 cut(s)	197, 229
PstNI	CAGNNNCTG	1 cut(s)	77
PsyI	GACNNNGTC	2 cut(s)	802, 1505
RsaI	GTAC	6 cut(s)	5, 206, 522, 598, 828, 961
RsaNI	GTAC	6 cut(s)	4, 205, 521, 597, 827, 960
RseI	CAYNNNNRTG	3 cut(s)	297, 834, 1556
SapI	GCTCTTC	1 cut(s)	271
SaqAI	TTAA	7 cut(s)	198, 752, 767, 929, 1008, 1287, 1521
SatI	GCNGC	4 cut(s)	81, 815, 842, 1563
Sau3AI	GATC	4 cut(s)	1180, 1274, 1353, 1581
ScaI	AGTACT	1 cut(s)	961
SchI	GAGTC	1 cut(s)	1098
ScrFI	CCNGG	2 cut(s)	189, 1041
SduI	GDGCHC	3 cut(s)	117, 134, 982
SfaNI	GCATC	2 cut(s)	649, 783
Sfr274I	CTCGAG	2 cut(s)	683, 894
SinI	GGWCC	6 cut(s)	68, 173, 185, 513, 866, 989
SlaI	CTCGAG	2 cut(s)	683, 894
SmiMI	CAYNNNNRTG	3 cut(s)	297, 834, 1556
SmlI	CTYRAG	3 cut(s)	683, 732, 894
SmoI	CTYRAG	3 cut(s)	683, 732, 894
SnaBI	TACGTA	1 cut(s)	1250
SspI	AATATT	2 cut(s)	487, 952
SspMI	CTAG	3 cut(s)	600, 1100, 1594
StyD4I	CCNGG	2 cut(s)	187, 1039
StyI	CCWWGG	3 cut(s)	516, 1214, 1474
TaaI	ACNGT	2 cut(s)	44, 616
TaiI	ACGT	5 cut(s)	33, 946, 1211, 1252, 1431
TaqI	TCGA	4 cut(s)	247, 284, 684, 895
TaqII	GACCGA	1 cut(s)	233
TatI	WGTACW	1 cut(s)	959
TauI	GCSGC	1 cut(s)	83
TfiI	GAWTC	1 cut(s)	643
Tru1I	TTAA	7 cut(s)	198, 752, 767, 929, 1008, 1287, 1521
Tru9I	TTAA	7 cut(s)	198, 752, 767, 929, 1008, 1287, 1521
TscAI	CASTG	1 cut(s)	564
TseFI	GTSAC	4 cut(s)	56, 1090, 1324, 1421
TseI	GCWGC	3 cut(s)	814, 841, 1562
Tsp45I	GTSAC	4 cut(s)	56, 1090, 1324, 1421
TspGWI	ACGGA	3 cut(s)	111, 141, 1343
TspRI	CASTG	1 cut(s)	564
Tth111I	GACNNNGTC	2 cut(s)	802, 1505
VpaK11BI	GGWCC	6 cut(s)	68, 173, 185, 513, 866, 989
XapI	RAATTY	6 cut(s)	535, 664, 912, 932, 1078, 1389
XceI	RCATGY	1 cut(s)	296
XhoI	CTCGAG	2 cut(s)	683, 894
XspI	CTAG	3 cut(s)	600, 1100, 1594
ZraI	GACGTC	1 cut(s)	31
ZrmI	AGTACT	1 cut(s)	961

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.