MD06G1192200.v1.1

Protein PHYTOCHROME KINASE SUBSTRATE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0015881
Physical Location & Seq
Reverse (-)
32781968 .. 32782855
888 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1192200.v1.1.491

Sequence Viewer

Length: 888 bp
ATGGAAAGAGAAGCCAACAACGGTACTCGTACTCAGGACGCTTCATTTTCTTCTTACCTCAGAAGGGCAGAAGGTAACTTTGTAGTCAAGTTTGCAGGATCAGTTCATATTCCGAATCCCCCTCCTGCCACGAAGACTGAGGGTGAAACCGGCGTCTTTGGTGCGGAGAAATATTTCAGCATGATGGTGGAAGATGACGGTCCACGACTTGTGGACTATGACACAAGCAAATTTGGGCAGAAGAAGAAAGAAAATAGGGGTGGTCAGCAGCACAAGCAACCAAAGAGCAGGCCAGGAACTCCGAGTACTTGTTCTGAAGCGAGTTGGAACAGCCAAAGCGGCTTGTTATCTTGGATGAGGACCCCGTCTCAGAGCAAGAAAAAAAAGGTAAATGGAAAGATCAATATCTTTTCGAGTTTTAGCTGCAATGGATCTTGCTCAGATAAGAAATCCATATCTGTTCATGAGATGAATGCCGGGCATGGAGGTCACGGCGATAAAGATTTTAGGAAGCAAAACGTTGGGAATATTGATCACAATCCTGCTAAGCAGCCTCAACCAAGATTTAAGGCAAGAGATGAGGTGTACTACTCCAGCTTTGAAAGGTCAAACAAGGAAGAGCATTCCGCATTGGCAAATCTGAACTCCGGTGCGCAGAAATTGTCAGGTAAATCACAGTTGGAAGAGAAGAAAATGAAAGAACAAGAAGGTCCCCGGAAATTGCTCGAGGTGTTTGGATCCCAAAGGATGAAGGAAGACAATGTCGCAATAAATTCAGAGAGGAGATCGTCCATGCTAACTTCGGATGCCATTCCAAAAGCTCCAGTGATGAATGAAGATGCCGAGAGTGATGCTAGTTCTGATTTGTTCGAGATTGAGAGCCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005911 GO:0009506 GO:0030054 GO:0055044
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

296

Amino Acids

32.73

Weight (kDa)

8.92

Isoelectric Point (pI)

51.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015881)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18810
fragaria_vesca FvH4_5g15090
malus_domestica MD06G1192200.v1.1 MD14G1198800.v1.1
prunus_persica Prupe.5G191900_v2.0.a1
pyrus_communis pycom06g17030
rosa_chinensis RchiOBHm_Chr7g0181121
rosa_laevigata RLG00000005212
rosa_roxburghii Rroxscaffold_3G00272260
rosa_rugosa Rorug06G0438700
rosa_samantha Rh7AG040500 Rh7BG039900 Rh7CG041900 Rh7DG040300
rosa_wichuraiana Rw7G003340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 654
AciI CCGC 3 cut(s) 164, 339, 627
AclI AACGTT 1 cut(s) 519
AclWI GGATC 4 cut(s) 106, 439, 732, 745
AcsI RAATTY 2 cut(s) 230, 772
AcuI CTGAAG 1 cut(s) 336
AcyI GRCGYC 1 cut(s) 153
AfaI GTAC 4 cut(s) 25, 31, 307, 587
AfiI CCNNNNNNNGG 1 cut(s) 64
AgsI TTSAA 1 cut(s) 602
AjnI CCWGG 1 cut(s) 292
AluBI AGCT 3 cut(s) 423, 597, 821
AluI AGCT 3 cut(s) 423, 597, 821
Alw26I GTCTC 1 cut(s) 372
AlwI GGATC 4 cut(s) 106, 439, 732, 745
Ama87I CYCGRG 1 cut(s) 725
AoxI GGCC 1 cut(s) 290
ApeKI GCWGC 3 cut(s) 268, 423, 550
ApoI RAATTY 2 cut(s) 230, 772
Asp700I GAANNNNTTC 1 cut(s) 173
AspLEI GCGC 1 cut(s) 655
AspS9I GGNCC 3 cut(s) 200, 360, 710
AsuC2I CCSGG 2 cut(s) 478, 715
AsuHPI GGTGA 1 cut(s) 155
AvaI CYCGRG 1 cut(s) 725
AvaII GGWCC 3 cut(s) 200, 360, 710
BamHI GGATCC 1 cut(s) 737
BbsI GAAGAC 2 cut(s) 140, 762
BbvI GCAGC 3 cut(s) 280, 410, 562
BccI CCATC 1 cut(s) 178
BceAI ACGGC 1 cut(s) 508
BcgI CGANNNNNNTGC 2 cut(s) 833, 867
BciT130I CCWGG 1 cut(s) 294
BclI TGATCA 1 cut(s) 532
BcnI CCSGG 2 cut(s) 478, 715
BcoDI GTCTC 1 cut(s) 372
BfaI CTAG 1 cut(s) 855
BglI GCCNNNNNGGC 1 cut(s) 339
BisI GCNGC 4 cut(s) 269, 340, 424, 551
BlpI GCTNAGC 1 cut(s) 546
BlsI GCNGC 4 cut(s) 270, 341, 425, 552
BmcAI AGTACT 1 cut(s) 307
Bme1390I CCNGG 3 cut(s) 294, 478, 715
Bme18I GGWCC 3 cut(s) 200, 360, 710
BmeT110I CYCGRG 1 cut(s) 725
BmgT120I GGNCC 3 cut(s) 200, 360, 710
BmiI GGNNCC 3 cut(s) 362, 712, 739
BmrFI CCNGG 3 cut(s) 294, 478, 715
BmsI GCATC 3 cut(s) 796, 829, 841
BpiI GAAGAC 2 cut(s) 140, 762
BpmI CTGGAG 2 cut(s) 577, 807
Bpu1102I GCTNAGC 1 cut(s) 546
BpuMI CCSGG 2 cut(s) 478, 715
BsaBI GATNNNNATC 2 cut(s) 404, 537
BsaHI GRCGYC 1 cut(s) 153
BsaJI CCNNGG 1 cut(s) 713
BsaWI WCCGGW 1 cut(s) 647
Bsc4I CCNNNNNNNGG 1 cut(s) 64
Bse118I RCCGGY 1 cut(s) 149
Bse1I ACTGG 1 cut(s) 824
Bse3DI GCAATG 1 cut(s) 433
Bse8I GATNNNNATC 2 cut(s) 404, 537
BseBI CCWGG 1 cut(s) 294
BseDI CCNNGG 1 cut(s) 713
BseGI GGATG 3 cut(s) 360, 753, 811
BseJI GATNNNNATC 2 cut(s) 404, 537
BseLI CCNNNNNNNGG 1 cut(s) 64
BseMI GCAATG 1 cut(s) 433
BseMII CTCAG 5 cut(s) 47, 73, 129, 383, 453
BseNI ACTGG 1 cut(s) 824
BseRI GAGGAG 1 cut(s) 796
BseXI GCAGC 3 cut(s) 280, 410, 562
BshFI GGCC 1 cut(s) 292
BsiHKCI CYCGRG 1 cut(s) 725
BsiSI CCGG 4 cut(s) 150, 477, 648, 715
BslFI GGGAC 1 cut(s) 696
BslI CCNNNNNNNGG 1 cut(s) 64
BsmAI GTCTC 1 cut(s) 372
BsmBI CGTCTC 1 cut(s) 372
BsmFI GGGAC 1 cut(s) 696
BsmI GAATGC 2 cut(s) 478, 622
BsnI GGCC 1 cut(s) 292
BsoBI CYCGRG 1 cut(s) 725
Bsp143I GATC 6 cut(s) 98, 399, 431, 532, 737, 785
Bsp1720I GCTNAGC 1 cut(s) 546
BspACI CCGC 3 cut(s) 164, 339, 627
BspANI GGCC 1 cut(s) 292
BspCNI CTCAG 5 cut(s) 46, 72, 130, 382, 452
BspHI TCATGA 1 cut(s) 463
BspLI GGNNCC 3 cut(s) 362, 712, 739
BspPI GGATC 4 cut(s) 106, 439, 732, 745
BspQI GCTCTTC 1 cut(s) 612
BsrDI GCAATG 1 cut(s) 433
BsrFI RCCGGY 1 cut(s) 149
BsrI ACTGG 1 cut(s) 824
BssAI RCCGGY 1 cut(s) 149
BssECI CCNNGG 1 cut(s) 713
BssMI GATC 6 cut(s) 98, 399, 431, 532, 737, 785
BssNI GRCGYC 1 cut(s) 153
Bst2UI CCWGG 1 cut(s) 294
Bst4CI ACNGT 3 cut(s) 23, 200, 678
Bst6I CTCTTC 2 cut(s) 612, 678
BstACI GRCGYC 1 cut(s) 153
BstC8I GCNNGC 1 cut(s) 290
BstDEI CTNAG 6 cut(s) 33, 59, 138, 369, 439, 546
BstF5I GGATG 3 cut(s) 360, 753, 811
BstHHI GCGC 1 cut(s) 655
BstKTI GATC 6 cut(s) 101, 402, 434, 535, 740, 788
BstMAI GTCTC 1 cut(s) 372
BstMBI GATC 6 cut(s) 98, 399, 431, 532, 737, 785
BstMWI GCNNNNNNNGC 2 cut(s) 274, 339
BstNI CCWGG 1 cut(s) 294
BstSCI CCNGG 3 cut(s) 292, 476, 713
BstV1I GCAGC 3 cut(s) 280, 410, 562
BstV2I GAAGAC 2 cut(s) 140, 762
BstX2I RGATCY 2 cut(s) 431, 737
BstYI RGATCY 2 cut(s) 431, 737
BsuRI GGCC 1 cut(s) 292
BtsCI GGATG 3 cut(s) 360, 753, 811
BtsIMutI CAGTG 1 cut(s) 831
Cac8I GCNNGC 1 cut(s) 290
CciI TCATGA 1 cut(s) 463
CfoI GCGC 1 cut(s) 655
Cfr10I RCCGGY 1 cut(s) 149
Cfr13I GGNCC 3 cut(s) 200, 360, 710
CseI GACGC 2 cut(s) 47, 142
Csp6I GTAC 4 cut(s) 24, 30, 306, 586
CviAII CATG 4 cut(s) 181, 464, 482, 793
CviJI RGCY 9 cut(s) 14, 292, 333, 342, 423, 553, 597, 821, 882
CviKI_1 RGCY 9 cut(s) 14, 292, 333, 342, 423, 553, 597, 821, 882
CviQI GTAC 4 cut(s) 24, 30, 306, 586
DdeI CTNAG 6 cut(s) 33, 59, 138, 369, 439, 546
DpnI GATC 6 cut(s) 100, 401, 433, 534, 739, 787
DpnII GATC 6 cut(s) 98, 399, 431, 532, 737, 785
Eam1104I CTCTTC 2 cut(s) 612, 678
EarI CTCTTC 2 cut(s) 612, 678
Eco47I GGWCC 3 cut(s) 200, 360, 710
Eco57I CTGAAG 1 cut(s) 336
Eco88I CYCGRG 1 cut(s) 725
EcoO109I RGGNCCY 2 cut(s) 360, 710
EcoRII CCWGG 1 cut(s) 292
Esp3I CGTCTC 1 cut(s) 372
FaeI CATG 4 cut(s) 184, 467, 485, 796
FaiI YATR 8 cut(s) 108, 182, 219, 455, 465, 483, 794, 886
FaqI GGGAC 1 cut(s) 696
FatI CATG 4 cut(s) 180, 463, 481, 792
FbaI TGATCA 1 cut(s) 532
Fnu4HI GCNGC 4 cut(s) 269, 340, 424, 551
FokI GGATG 3 cut(s) 367, 760, 818
Fsp4HI GCNGC 4 cut(s) 269, 340, 424, 551
FspBI CTAG 1 cut(s) 855
FspI TGCGCA 1 cut(s) 654
GlaI GCGC 1 cut(s) 654
GluI GCNGC 4 cut(s) 269, 340, 424, 551
GsuI CTGGAG 2 cut(s) 577, 807
HaeIII GGCC 1 cut(s) 292
HapII CCGG 4 cut(s) 150, 477, 648, 715
HgaI GACGC 2 cut(s) 47, 142
HhaI GCGC 1 cut(s) 655
Hin1I GRCGYC 1 cut(s) 153
Hin1II CATG 4 cut(s) 184, 467, 485, 796
Hin6I GCGC 1 cut(s) 653
HinP1I GCGC 1 cut(s) 653
HinfI GANTC 1 cut(s) 115
HpaII CCGG 4 cut(s) 150, 477, 648, 715
HphI GGTGA 1 cut(s) 155
Hpy166II GTNNAC 3 cut(s) 203, 214, 586
Hpy188III TCNNGA 3 cut(s) 35, 464, 871
Hpy8I GTNNAC 3 cut(s) 203, 214, 586
HpyAV CCTTC 4 cut(s) 57, 65, 701, 745
HpyCH4III ACNGT 3 cut(s) 23, 200, 678
HpyCH4IV ACGT 1 cut(s) 519
HpyCH4V TGCA 2 cut(s) 95, 426
HpyF10VI GCNNNNNNNGC 2 cut(s) 274, 339
HpyF3I CTNAG 6 cut(s) 33, 59, 138, 369, 439, 546
HpySE526I ACGT 1 cut(s) 519
Hsp92I GRCGYC 1 cut(s) 153
Hsp92II CATG 4 cut(s) 184, 467, 485, 796
HspAI GCGC 1 cut(s) 653
Ksp22I TGATCA 1 cut(s) 532
Kzo9I GATC 6 cut(s) 98, 399, 431, 532, 737, 785
LguI GCTCTTC 1 cut(s) 612
LmnI GCTCC 1 cut(s) 826
Lsp1109I GCAGC 3 cut(s) 280, 410, 562
LweI GCATC 3 cut(s) 796, 829, 841
MaeI CTAG 1 cut(s) 855
MaeII ACGT 1 cut(s) 519
MaeIII GTNAC 2 cut(s) 74, 488
MalI GATC 6 cut(s) 100, 401, 433, 534, 739, 787
MboI GATC 6 cut(s) 98, 399, 431, 532, 737, 785
MflI RGATCY 2 cut(s) 431, 737
MluCI AATT 4 cut(s) 230, 659, 719, 772
MmeI TCCRAC 2 cut(s) 305, 660
MnlI CCTC 9 cut(s) 68, 132, 133, 351, 479, 564, 574, 721, 774
MroXI GAANNNNTTC 1 cut(s) 173
MseI TTAA 1 cut(s) 567
MslI CAYNNNNRTG 1 cut(s) 185
MspI CCGG 4 cut(s) 150, 477, 648, 715
MspR9I CCNGG 3 cut(s) 294, 478, 715
Mva1269I GAATGC 2 cut(s) 478, 622
MvaI CCWGG 1 cut(s) 294
MwoI GCNNNNNNNGC 2 cut(s) 274, 339
NciI CCSGG 2 cut(s) 478, 715
NdeII GATC 6 cut(s) 98, 399, 431, 532, 737, 785
NlaIII CATG 4 cut(s) 184, 467, 485, 796
NlaIV GGNNCC 3 cut(s) 362, 712, 739
NmeAIII GCCGAG 1 cut(s) 868
NmuCI GTSAC 1 cut(s) 488
NsbI TGCGCA 1 cut(s) 654
PaeR7I CTCGAG 1 cut(s) 725
PagI TCATGA 1 cut(s) 463
PciSI GCTCTTC 1 cut(s) 612
PctI GAATGC 2 cut(s) 478, 622
PdmI GAANNNNTTC 1 cut(s) 173
PfeI GAWTC 1 cut(s) 115
PflFI GACNNNGTC 2 cut(s) 364, 761
PkrI GCNGC 4 cut(s) 270, 341, 425, 552
PpuMI RGGWCCY 2 cut(s) 360, 710
Psp1406I AACGTT 1 cut(s) 519
Psp5II RGGWCCY 2 cut(s) 360, 710
Psp6I CCWGG 1 cut(s) 292
PspGI CCWGG 1 cut(s) 292
PspN4I GGNNCC 3 cut(s) 362, 712, 739
PspPI GGNCC 3 cut(s) 200, 360, 710
PspPPI RGGWCCY 2 cut(s) 360, 710
PspXI VCTCGAGB 1 cut(s) 725
PsrI GAACNNNNNNTAC 2 cut(s) 289, 321
PsuI RGATCY 2 cut(s) 431, 737
PsyI GACNNNGTC 2 cut(s) 364, 761
RsaI GTAC 4 cut(s) 25, 31, 307, 587
RsaNI GTAC 4 cut(s) 24, 30, 306, 586
RseI CAYNNNNRTG 1 cut(s) 185
SapI GCTCTTC 1 cut(s) 612
SaqAI TTAA 1 cut(s) 567
SatI GCNGC 4 cut(s) 269, 340, 424, 551
Sau3AI GATC 6 cut(s) 98, 399, 431, 532, 737, 785
Sau96I GGNCC 3 cut(s) 200, 360, 710
ScaI AGTACT 1 cut(s) 307
ScrFI CCNGG 3 cut(s) 294, 478, 715
SfaNI GCATC 3 cut(s) 796, 829, 841
Sfr274I CTCGAG 1 cut(s) 725
SinI GGWCC 3 cut(s) 200, 360, 710
SlaI CTCGAG 1 cut(s) 725
SmiMI CAYNNNNRTG 1 cut(s) 185
SmlI CTYRAG 1 cut(s) 725
SmoI CTYRAG 1 cut(s) 725
Sse9I AATT 4 cut(s) 230, 659, 719, 772
SsiI CCGC 3 cut(s) 164, 339, 627
SspI AATATT 2 cut(s) 173, 529
SspMI CTAG 1 cut(s) 855
StyD4I CCNGG 3 cut(s) 292, 476, 713
TaaI ACNGT 3 cut(s) 23, 200, 678
TaiI ACGT 1 cut(s) 522
TaqI TCGA 3 cut(s) 413, 726, 870
TasI AATT 4 cut(s) 230, 659, 719, 772
TatI WGTACW 2 cut(s) 305, 585
TauI GCSGC 1 cut(s) 342
TfiI GAWTC 1 cut(s) 115
Tru1I TTAA 1 cut(s) 567
Tru9I TTAA 1 cut(s) 567
TscAI CASTG 1 cut(s) 831
TseFI GTSAC 1 cut(s) 488
TseI GCWGC 3 cut(s) 268, 423, 550
Tsp45I GTSAC 1 cut(s) 488
TspDTI ATGAA 8 cut(s) 33, 95, 452, 485, 710, 764, 845, 849
TspRI CASTG 1 cut(s) 831
Tth111I GACNNNGTC 2 cut(s) 364, 761
VpaK11BI GGWCC 3 cut(s) 200, 360, 710
XapI RAATTY 2 cut(s) 230, 772
XhoI CTCGAG 1 cut(s) 725
XmnI GAANNNNTTC 1 cut(s) 173
XspI CTAG 1 cut(s) 855
ZrmI AGTACT 1 cut(s) 307
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.