Rw7G003340

Protein PHYTOCHROME KINASE SUBSTRATE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0015881
Physical Location & Seq
Reverse (-)
2697057 .. 2698374
1318 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G003340.1

Sequence Viewer

Length: 1095 bp
ATGGAAAGAGAGGCTAACAACAACACTAATCATACCCGCGACGCCTCGTTTGCTTCTTACCTCACCAGAGCAGAAGGGAACTTGGTGCTCAAGTCTCCTGCCTTGAACTTGAGCCAAAAGACCGATCAGGAGGGCGAAATCAGTGTCTTCGGCGCTGAAAGATATTTCAGCATGATGCTGGAAGATGACAGTCCAAGAGTACTTGTGGACTATAACAACACCAGCAGAGCTGGTCTGCAGCGACTGAAGCAACCAAAGACTAGTAGGCCAGGAACTCCTAGTACTTGTTCTAATGCAAGCTGGAACATTCAAACCGCTTTCTTGCCTTCTCGGAGAAGAAACCAGTCTCAGAACGAGAAGAAAAAGGTGAATGGAAAGAAGATATTTTCCGGTTTTGGCTGCAATGGTTCTTGTTCAGATCAGAAATCCATTCATGTTCATGAGGTTAATGTACGTGTTGCTAGAGATTTCAGGAAACTAAGTTTTGGAAGTGATCACCATCCTGTCAAGCAGTCTCCACCAAGAATTAAGGCCAAAGATCACGAGCTTCTTCACCGCTACCCTAGCTTTGGAGGATCAAACAAAAAACAACATTTTCGGTTTACAAATTTTGGAGCTAAAGAAGAGAAGAAGACAAAACTAGAAGAAGAAGAGCCACAAAAATCGCTGGAAGTGTTTGGATCGGAAATGAAGAAGGAAGGAGAAGACTTAGTGGCAATAAATCTAGAGAGGAGACTTTCCATGTTATCTTGGGATGCCATTCCAAAAGCTCAAAGCCACTCAATTACTTCTGGAAATAGTGAAATGATCAATGAAGATATTGAGAGTGATGCTAGTTCTGATTTGTTTGAGATTGAGAACTTAACCGGCACCTGTCAGCCATTAATGTTCACAAAGCAAGCATCTGATGGATTGTCCATGGCTTCCCCCACCAAGTATGACTCAAGTGAGACCAGCATTGACTGTAGTGTTGTGACAGCTAATTTTATCTCAGCTGCAGTTTCAGATTACGATGAGAAGAAATTAGTGCCGGCCAACCAACTGCAACATCAAAGCCTGAATTCTTCAATGGAAGTAGGCAAAGTTACAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005911 GO:0009506 GO:0030054 GO:0055044
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

364

Amino Acids

40.67

Weight (kDa)

7.15

Isoelectric Point (pI)

47.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015881)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18810
fragaria_vesca FvH4_5g15090
malus_domestica MD06G1192200.v1.1 MD14G1198800.v1.1
prunus_persica Prupe.5G191900_v2.0.a1
pyrus_communis pycom06g17030
rosa_chinensis RchiOBHm_Chr7g0181121
rosa_laevigata RLG00000005212
rosa_roxburghii Rroxscaffold_3G00272260
rosa_rugosa Rorug06G0438700
rosa_samantha Rh7AG040500 Rh7BG039900 Rh7CG041900 Rh7DG040300
rosa_wichuraiana Rw7G003340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 869
AccII CGCG 1 cut(s) 39
AciI CCGC 3 cut(s) 37, 315, 556
AclWI GGATC 2 cut(s) 583, 688
AcoI YGGCCR 1 cut(s) 1032
AcsI RAATTY 2 cut(s) 607, 1060
AcuI CTGAAG 1 cut(s) 266
AcyI GRCGYC 1 cut(s) 42
AfaI GTAC 3 cut(s) 201, 283, 453
AfiI CCNNNNNNNGG 1 cut(s) 569
AflIII ACRYGT 1 cut(s) 454
AgsI TTSAA 3 cut(s) 106, 311, 1068
AhlI ACTAGT 1 cut(s) 260
AjnI CCWGG 1 cut(s) 268
AluBI AGCT 8 cut(s) 230, 300, 547, 567, 617, 770, 980, 995
AluI AGCT 8 cut(s) 230, 300, 547, 567, 617, 770, 980, 995
Alw21I GWGCWC 1 cut(s) 90
Alw26I GTCTC 5 cut(s) 99, 351, 519, 727, 944
AlwI GGATC 2 cut(s) 583, 688
AlwNI CAGNNNCTG 1 cut(s) 244
AoxI GGCC 3 cut(s) 266, 531, 1032
ApeKI GCWGC 3 cut(s) 238, 399, 995
ApoI RAATTY 2 cut(s) 607, 1060
ArsI GACNNNNNNTTYG 4 cut(s) 32, 64, 129, 161
AseI ATTAAT 1 cut(s) 884
AspLEI GCGC 1 cut(s) 155
AsuHPI GGTGA 4 cut(s) 55, 379, 488, 545
BanI GGYRCC 1 cut(s) 869
BauI CACGAG 1 cut(s) 542
BbsI GAAGAC 3 cut(s) 139, 638, 711
Bbv12I GWGCWC 1 cut(s) 90
BbvI GCAGC 3 cut(s) 250, 386, 982
BccI CCATC 2 cut(s) 507, 902
BciT130I CCWGG 1 cut(s) 270
BclI TGATCA 2 cut(s) 493, 807
BcoDI GTCTC 5 cut(s) 99, 351, 519, 727, 944
BcuI ACTAGT 1 cut(s) 260
BfaI CTAG 7 cut(s) 261, 279, 462, 564, 641, 725, 834
BfmI CTRYAG 3 cut(s) 236, 964, 996
BfoI RGCGCY 1 cut(s) 156
BisI GCNGC 3 cut(s) 239, 400, 996
BlsI GCNGC 3 cut(s) 240, 401, 997
BmcAI AGTACT 2 cut(s) 201, 283
Bme1390I CCNGG 1 cut(s) 270
BmiI GGNNCC 1 cut(s) 871
BmrFI CCNGG 1 cut(s) 270
BmsI GCATC 4 cut(s) 165, 745, 820, 911
BpiI GAAGAC 3 cut(s) 139, 638, 711
BpuEI CTTGAG 3 cut(s) 74, 130, 928
BsaAI YACGTR 1 cut(s) 455
BsaBI GATNNNNATC 1 cut(s) 498
BsaHI GRCGYC 1 cut(s) 42
BsaI GGTCTC 1 cut(s) 944
BsaJI CCNNGG 1 cut(s) 918
BsaWI WCCGGW 1 cut(s) 389
Bsc4I CCNNNNNNNGG 1 cut(s) 569
Bse118I RCCGGY 2 cut(s) 866, 1030
Bse1I ACTGG 1 cut(s) 343
Bse3DI GCAATG 1 cut(s) 409
Bse8I GATNNNNATC 1 cut(s) 498
BseBI CCWGG 1 cut(s) 270
BseDI CCNNGG 1 cut(s) 918
BseGI GGATG 2 cut(s) 499, 760
BseJI GATNNNNATC 1 cut(s) 498
BseLI CCNNNNNNNGG 1 cut(s) 569
BseMI GCAATG 1 cut(s) 409
BseMII CTCAG 2 cut(s) 362, 1005
BseNI ACTGG 1 cut(s) 343
BseRI GAGGAG 1 cut(s) 745
BseXI GCAGC 3 cut(s) 250, 386, 982
Bsh1236I CGCG 1 cut(s) 39
BshFI GGCC 3 cut(s) 268, 533, 1034
BshNI GGYRCC 1 cut(s) 869
BsiHKAI GWGCWC 1 cut(s) 90
BsiSI CCGG 3 cut(s) 390, 867, 1031
BslI CCNNNNNNNGG 1 cut(s) 569
BsmAI GTCTC 5 cut(s) 99, 351, 519, 727, 944
BsnI GGCC 3 cut(s) 268, 533, 1034
Bso31I GGTCTC 1 cut(s) 944
Bsp1286I GDGCHC 1 cut(s) 90
Bsp143I GATC 7 cut(s) 124, 418, 493, 538, 575, 680, 807
Bsp19I CCATGG 1 cut(s) 918
BspACI CCGC 3 cut(s) 37, 315, 556
BspANI GGCC 3 cut(s) 268, 533, 1034
BspCNI CTCAG 2 cut(s) 361, 1004
BspFNI CGCG 1 cut(s) 39
BspHI TCATGA 1 cut(s) 439
BspLI GGNNCC 1 cut(s) 871
BspMAI CTGCAG 2 cut(s) 240, 1000
BspPI GGATC 2 cut(s) 583, 688
BspQI GCTCTTC 1 cut(s) 645
BspT107I GGYRCC 1 cut(s) 869
BspTNI GGTCTC 1 cut(s) 944
BsrDI GCAATG 1 cut(s) 409
BsrFI RCCGGY 2 cut(s) 866, 1030
BsrI ACTGG 1 cut(s) 343
BssAI RCCGGY 2 cut(s) 866, 1030
BssECI CCNNGG 1 cut(s) 918
BssMI GATC 7 cut(s) 124, 418, 493, 538, 575, 680, 807
BssNI GRCGYC 1 cut(s) 42
BssSI CACGAG 1 cut(s) 542
BssT1I CCWWGG 1 cut(s) 918
Bst2BI CACGAG 1 cut(s) 542
Bst2UI CCWGG 1 cut(s) 270
Bst4CI ACNGT 2 cut(s) 191, 965
Bst6I CTCTTC 2 cut(s) 618, 645
BstACI GRCGYC 1 cut(s) 42
BstBAI YACGTR 1 cut(s) 455
BstC8I GCNNGC 3 cut(s) 298, 900, 1032
BstDEI CTNAG 4 cut(s) 348, 479, 709, 991
BstDSI CCRYGG 1 cut(s) 918
BstF5I GGATG 2 cut(s) 499, 760
BstFNI CGCG 1 cut(s) 39
BstH2I RGCGCY 1 cut(s) 156
BstHHI GCGC 1 cut(s) 155
BstKTI GATC 7 cut(s) 127, 421, 496, 541, 578, 683, 810
BstMAI GTCTC 5 cut(s) 99, 351, 519, 727, 944
BstMBI GATC 7 cut(s) 124, 418, 493, 538, 575, 680, 807
BstMWI GCNNNNNNNGC 3 cut(s) 50, 247, 564
BstNI CCWGG 1 cut(s) 270
BstSCI CCNGG 1 cut(s) 268
BstSFI CTRYAG 3 cut(s) 236, 964, 996
BstUI CGCG 1 cut(s) 39
BstV1I GCAGC 3 cut(s) 250, 386, 982
BstV2I GAAGAC 3 cut(s) 139, 638, 711
BsuRI GGCC 3 cut(s) 268, 533, 1034
BtgI CCRYGG 1 cut(s) 918
BtsCI GGATG 2 cut(s) 499, 760
BtsIMutI CAGTG 1 cut(s) 148
Cac8I GCNNGC 3 cut(s) 298, 900, 1032
CaiI CAGNNNCTG 1 cut(s) 244
CciI TCATGA 1 cut(s) 439
CfoI GCGC 1 cut(s) 155
Cfr10I RCCGGY 2 cut(s) 866, 1030
CseI GACGC 1 cut(s) 50
Csp6I GTAC 3 cut(s) 200, 282, 452
CviAII CATG 5 cut(s) 172, 434, 440, 742, 919
CviQI GTAC 3 cut(s) 200, 282, 452
DdeI CTNAG 4 cut(s) 348, 479, 709, 991
DpnI GATC 7 cut(s) 126, 420, 495, 540, 577, 682, 809
DpnII GATC 7 cut(s) 124, 418, 493, 538, 575, 680, 807
EaeI YGGCCR 1 cut(s) 1032
Eam1104I CTCTTC 2 cut(s) 618, 645
EarI CTCTTC 2 cut(s) 618, 645
Eco130I CCWWGG 1 cut(s) 918
Eco31I GGTCTC 1 cut(s) 944
Eco57I CTGAAG 1 cut(s) 266
EcoRI GAATTC 1 cut(s) 1060
EcoRII CCWGG 1 cut(s) 268
EcoT14I CCWWGG 1 cut(s) 918
ErhI CCWWGG 1 cut(s) 918
FaeI CATG 5 cut(s) 175, 437, 443, 745, 922
FaiI YATR 8 cut(s) 33, 173, 213, 435, 441, 743, 920, 939
FatI CATG 5 cut(s) 171, 433, 439, 741, 918
FauI CCCGC 1 cut(s) 44
FbaI TGATCA 2 cut(s) 493, 807
Fnu4HI GCNGC 3 cut(s) 239, 400, 996
FokI GGATG 2 cut(s) 486, 767
Fsp4HI GCNGC 3 cut(s) 239, 400, 996
FspBI CTAG 7 cut(s) 261, 279, 462, 564, 641, 725, 834
GlaI GCGC 1 cut(s) 154
GluI GCNGC 3 cut(s) 239, 400, 996
HaeII RGCGCY 1 cut(s) 156
HaeIII GGCC 3 cut(s) 268, 533, 1034
HapII CCGG 3 cut(s) 390, 867, 1031
HgaI GACGC 1 cut(s) 50
HhaI GCGC 1 cut(s) 155
Hin1I GRCGYC 1 cut(s) 42
Hin1II CATG 5 cut(s) 175, 437, 443, 745, 922
Hin6I GCGC 1 cut(s) 153
HinP1I GCGC 1 cut(s) 153
HinfI GANTC 1 cut(s) 941
HpaII CCGG 3 cut(s) 390, 867, 1031
HphI GGTGA 4 cut(s) 55, 379, 488, 545
Hpy166II GTNNAC 3 cut(s) 208, 603, 891
Hpy188I TCNGA 9 cut(s) 333, 351, 418, 423, 685, 841, 907, 1006, 1094
Hpy188III TCNNGA 6 cut(s) 128, 440, 472, 542, 725, 792
Hpy8I GTNNAC 3 cut(s) 208, 603, 891
Hpy99I CGWCG 1 cut(s) 44
HpyAV CCTTC 4 cut(s) 68, 336, 688, 692
HpyCH4III ACNGT 2 cut(s) 191, 965
HpyCH4IV ACGT 1 cut(s) 454
HpyCH4V TGCA 5 cut(s) 238, 296, 402, 998, 1045
HpyF10VI GCNNNNNNNGC 3 cut(s) 50, 247, 564
HpyF3I CTNAG 4 cut(s) 348, 479, 709, 991
HpySE526I ACGT 1 cut(s) 454
Hsp92I GRCGYC 1 cut(s) 42
Hsp92II CATG 5 cut(s) 175, 437, 443, 745, 922
HspAI GCGC 1 cut(s) 153
KroI GCCGGC 1 cut(s) 1030
KroNI GCCGGC 1 cut(s) 1032
Ksp22I TGATCA 2 cut(s) 493, 807
Kzo9I GATC 7 cut(s) 124, 418, 493, 538, 575, 680, 807
LguI GCTCTTC 1 cut(s) 645
LmnI GCTCC 1 cut(s) 614
Lsp1109I GCAGC 3 cut(s) 250, 386, 982
LweI GCATC 4 cut(s) 165, 745, 820, 911
MaeI CTAG 7 cut(s) 261, 279, 462, 564, 641, 725, 834
MaeII ACGT 1 cut(s) 454
MaeIII GTNAC 2 cut(s) 973, 1084
MalI GATC 7 cut(s) 126, 420, 495, 540, 577, 682, 809
MboI GATC 7 cut(s) 124, 418, 493, 538, 575, 680, 807
MhlI GDGCHC 1 cut(s) 90
MluCI AATT 6 cut(s) 525, 607, 783, 982, 1022, 1060
MlyI GAGTC 1 cut(s) 935
MnlI CCTC 7 cut(s) 4, 55, 71, 124, 436, 566, 723
MroNI GCCGGC 1 cut(s) 1030
MseI TTAA 4 cut(s) 447, 528, 863, 884
MslI CAYNNNNRTG 1 cut(s) 438
MspA1I CMGCKG 1 cut(s) 995
MspI CCGG 3 cut(s) 390, 867, 1031
MspR9I CCNGG 1 cut(s) 270
MvaI CCWGG 1 cut(s) 270
MvnI CGCG 1 cut(s) 39
MwoI GCNNNNNNNGC 3 cut(s) 50, 247, 564
NaeI GCCGGC 1 cut(s) 1032
NcoI CCATGG 1 cut(s) 918
NdeII GATC 7 cut(s) 124, 418, 493, 538, 575, 680, 807
NgoMIV GCCGGC 1 cut(s) 1030
NlaIII CATG 5 cut(s) 175, 437, 443, 745, 922
NlaIV GGNNCC 1 cut(s) 871
NmuCI GTSAC 1 cut(s) 973
PagI TCATGA 1 cut(s) 439
PciSI GCTCTTC 1 cut(s) 645
PdiI GCCGGC 1 cut(s) 1032
PkrI GCNGC 3 cut(s) 240, 401, 997
PleI GAGTC 1 cut(s) 935
PpsI GAGTC 1 cut(s) 935
Ppu21I YACGTR 1 cut(s) 455
PshBI ATTAAT 1 cut(s) 884
Psp6I CCWGG 1 cut(s) 268
PspGI CCWGG 1 cut(s) 268
PspN4I GGNNCC 1 cut(s) 871
PsrI GAACNNNNNNTAC 2 cut(s) 265, 297
PstI CTGCAG 2 cut(s) 240, 1000
PstNI CAGNNNCTG 1 cut(s) 244
PvuII CAGCTG 1 cut(s) 995
RsaI GTAC 3 cut(s) 201, 283, 453
RsaNI GTAC 3 cut(s) 200, 282, 452
RseI CAYNNNNRTG 1 cut(s) 438
SapI GCTCTTC 1 cut(s) 645
SaqAI TTAA 4 cut(s) 447, 528, 863, 884
SatI GCNGC 3 cut(s) 239, 400, 996
Sau3AI GATC 7 cut(s) 124, 418, 493, 538, 575, 680, 807
ScaI AGTACT 2 cut(s) 201, 283
SchI GAGTC 1 cut(s) 935
ScrFI CCNGG 1 cut(s) 270
SduI GDGCHC 1 cut(s) 90
SfaNI GCATC 4 cut(s) 165, 745, 820, 911
SfcI CTRYAG 3 cut(s) 236, 964, 996
SmiMI CAYNNNNRTG 1 cut(s) 438
SmlI CTYRAG 3 cut(s) 89, 109, 943
SmoI CTYRAG 3 cut(s) 89, 109, 943
SpeI ACTAGT 1 cut(s) 260
Sse9I AATT 6 cut(s) 525, 607, 783, 982, 1022, 1060
SsiI CCGC 3 cut(s) 37, 315, 556
SspMI CTAG 7 cut(s) 261, 279, 462, 564, 641, 725, 834
StyD4I CCNGG 1 cut(s) 268
StyI CCWWGG 1 cut(s) 918
TaaI ACNGT 2 cut(s) 191, 965
TaiI ACGT 1 cut(s) 457
TaqII GACCGA 1 cut(s) 137
TasI AATT 6 cut(s) 525, 607, 783, 982, 1022, 1060
TatI WGTACW 2 cut(s) 199, 281
Tru1I TTAA 4 cut(s) 447, 528, 863, 884
Tru9I TTAA 4 cut(s) 447, 528, 863, 884
TscAI CASTG 1 cut(s) 148
TseFI GTSAC 1 cut(s) 973
TseI GCWGC 3 cut(s) 238, 399, 995
Tsp45I GTSAC 1 cut(s) 973
TspDTI ATGAA 4 cut(s) 422, 428, 704, 828
TspRI CASTG 1 cut(s) 148
VspI ATTAAT 1 cut(s) 884
XapI RAATTY 2 cut(s) 607, 1060
XbaI TCTAGA 1 cut(s) 724
XspI CTAG 7 cut(s) 261, 279, 462, 564, 641, 725, 834
ZrmI AGTACT 2 cut(s) 201, 283
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.