MD14G1198800.v1.1

Protein PHYTOCHROME KINASE SUBSTRATE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0015881
Physical Location & Seq
Reverse (-)
28880569 .. 28881603
1035 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1198800.v1.1.491

Sequence Viewer

Length: 1035 bp
ATGGAAGGAGACGCCAAGACCGGGATTCATACCCGGGACGCTTCGTTTTCTTCTTACCTCAACAGAGCAGAAGGTAACTTTGTGCTCAAGCTTGCAGGATCAGTTCAGACTCCGAATCCCCCTCCTGCCAAGAAGATTGAGGAGGGTGAAATCAGTGTCTTTGGCGCGGAGAAATATTTCAACATGATGCTGGAAGATGACGGTCCACGAATTGTGGACTATGACACAAGCAAACTTGGGCAGAAGAAGGAAGAAAATAGAGGTGGTCAGAAGCACAAGCAACCAAAGAGCAGGCCAGGCACTCCGAGTACTTGTTCTGAAGCGAGTTGGAACAGCCAAAGCGGCTTGTTACCTTCGCTGAGAACCCCGTCTCAGAGCAAGAAGAAAAAGAGAAATGGAAAGGTCAATATCTTTTCGGGTTTTAGCTGCAATGGATCTTGTTCAGATAAGAAATCCATTTATATTCATGAGATGAATGCCGGGCATGGAGGTCGCGGAAATAAAGATTTTAGGAAGCAAAATGTTGGGAATATTGATCACAATCCCGTTAAGCAGCCGCAGCCAAGATTTAAGGCGAGGGATGAGGTTTACTACTCTAGCTTTGAAAGATCAAACAAGGAAGAGCATTTTGCATTCGCAAATTTGAATTCCGGTGCAAAGAAATTGGCTGCTAAATCACAGTTGGAAGAGAAGAAAATGAAAGACGAAGATCCACGAAAATCACTGGAGGTGTTTGGATCCCAAAGTATGAAGGGAGACAATGTAGCAATAAATTTAGAGAGGAGATTGTCCATGCTAAAGTGGGATGCCATTCCGAAAGCTCCAATGATGAATGAAGATGCGGAGAGTGATGCGAGTTCGGATTTGTTCGAGATAGAGAGCCTATCGGGGAATGGGATACCTATGGCATCCGCCACCAGGGATGACTCAAGTGAGACCGGAATGGAGAAAATGGCTGTAAAAGGTCGAACACCGGCTCCAGCAACGACTGGCAAGGCAGTAGGAGTTGCTCAAAGGTTGGAACCTTCAATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005911 GO:0009506 GO:0030054 GO:0055044
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

345

Amino Acids

37.8

Weight (kDa)

9.15

Isoelectric Point (pI)

47.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015881)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18810
fragaria_vesca FvH4_5g15090
malus_domestica MD06G1192200.v1.1 MD14G1198800.v1.1
prunus_persica Prupe.5G191900_v2.0.a1
pyrus_communis pycom06g17030
rosa_chinensis RchiOBHm_Chr7g0181121
rosa_laevigata RLG00000005212
rosa_roxburghii Rroxscaffold_3G00272260
rosa_rugosa Rorug06G0438700
rosa_samantha Rh7AG040500 Rh7BG039900 Rh7CG041900 Rh7DG040300
rosa_wichuraiana Rw7G003340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 167, 495
AciI CCGC 6 cut(s) 167, 342, 495, 557, 842, 912
AclWI GGATC 5 cut(s) 106, 442, 704, 732, 745
AcsI RAATTY 3 cut(s) 640, 646, 772
AcuI CTGAAG 1 cut(s) 339
AcyI GRCGYC 1 cut(s) 12
AfaI GTAC 1 cut(s) 310
AfiI CCNNNNNNNGG 2 cut(s) 21, 918
AgsI TTSAA 4 cut(s) 181, 605, 646, 1029
AjnI CCWGG 2 cut(s) 295, 917
AloI GAACNNNNNNTCC 2 cut(s) 961, 993
AluBI AGCT 4 cut(s) 91, 426, 600, 821
AluI AGCT 4 cut(s) 91, 426, 600, 821
Alw21I GWGCWC 1 cut(s) 87
Alw26I GTCTC 4 cut(s) 3, 375, 750, 929
AlwI GGATC 5 cut(s) 106, 442, 704, 732, 745
Ama87I CYCGRG 1 cut(s) 33
AoxI GGCC 1 cut(s) 293
ApeKI GCWGC 4 cut(s) 426, 553, 559, 668
ApoI RAATTY 3 cut(s) 640, 646, 772
Asp700I GAANNNNTTC 1 cut(s) 176
AspLEI GCGC 1 cut(s) 167
AspS9I GGNCC 1 cut(s) 203
AsuC2I CCSGG 4 cut(s) 22, 34, 35, 481
AsuHPI GGTGA 1 cut(s) 158
AvaI CYCGRG 1 cut(s) 33
AvaII GGWCC 1 cut(s) 203
BamHI GGATCC 1 cut(s) 737
Bbv12I GWGCWC 1 cut(s) 87
BbvI GCAGC 4 cut(s) 413, 565, 571, 655
BcgI CGANNNNNNTGC 2 cut(s) 473, 507
BciT130I CCWGG 2 cut(s) 297, 919
BciVI GTATCC 1 cut(s) 891
BclI TGATCA 1 cut(s) 535
BcnI CCSGG 4 cut(s) 22, 34, 35, 481
BcoDI GTCTC 4 cut(s) 3, 375, 750, 929
BfaI CTAG 1 cut(s) 597
BfuI GTATCC 1 cut(s) 891
BglI GCCNNNNNGGC 1 cut(s) 342
BisI GCNGC 6 cut(s) 343, 427, 554, 557, 560, 669
BlsI GCNGC 6 cut(s) 344, 428, 555, 558, 561, 670
BmcAI AGTACT 1 cut(s) 310
Bme1390I CCNGG 6 cut(s) 22, 34, 35, 297, 481, 919
Bme18I GGWCC 1 cut(s) 203
BmeT110I CYCGRG 1 cut(s) 33
BmgT120I GGNCC 1 cut(s) 203
BmiI GGNNCC 3 cut(s) 739, 978, 1023
BmrFI CCNGG 6 cut(s) 22, 34, 35, 297, 481, 919
BmsI GCATC 5 cut(s) 177, 796, 829, 841, 917
BpmI CTGGAG 2 cut(s) 746, 963
BpuEI CTTGAG 2 cut(s) 71, 913
BpuMI CCSGG 4 cut(s) 22, 34, 35, 481
BsaBI GATNNNNATC 1 cut(s) 540
BsaHI GRCGYC 1 cut(s) 12
BsaI GGTCTC 1 cut(s) 929
BsaJI CCNNGG 2 cut(s) 33, 918
BsaWI WCCGGW 2 cut(s) 650, 938
BsaXI ACNNNNNCTCC 4 cut(s) 747, 777, 961, 991
Bsc4I CCNNNNNNNGG 2 cut(s) 21, 918
Bse118I RCCGGY 1 cut(s) 973
Bse1I ACTGG 2 cut(s) 729, 994
Bse3DI GCAATG 1 cut(s) 436
Bse8I GATNNNNATC 1 cut(s) 540
BseBI CCWGG 2 cut(s) 297, 919
BseDI CCNNGG 2 cut(s) 33, 918
BseGI GGATG 4 cut(s) 586, 811, 908, 928
BseJI GATNNNNATC 1 cut(s) 540
BseLI CCNNNNNNNGG 2 cut(s) 21, 918
BseMI GCAATG 1 cut(s) 436
BseMII CTCAG 2 cut(s) 350, 386
BseNI ACTGG 2 cut(s) 729, 994
BseRI GAGGAG 2 cut(s) 155, 796
BseXI GCAGC 4 cut(s) 413, 565, 571, 655
Bsh1236I CGCG 2 cut(s) 167, 495
BshFI GGCC 1 cut(s) 295
BsiHKAI GWGCWC 1 cut(s) 87
BsiHKCI CYCGRG 1 cut(s) 33
BsiSI CCGG 6 cut(s) 21, 34, 480, 651, 939, 974
BslFI GGGAC 1 cut(s) 50
BslI CCNNNNNNNGG 2 cut(s) 21, 918
BsmAI GTCTC 4 cut(s) 3, 375, 750, 929
BsmBI CGTCTC 2 cut(s) 3, 375
BsmFI GGGAC 1 cut(s) 50
BsmI GAATGC 2 cut(s) 481, 632
BsnI GGCC 1 cut(s) 295
Bso31I GGTCTC 1 cut(s) 929
BsoBI CYCGRG 1 cut(s) 33
Bsp1286I GDGCHC 1 cut(s) 87
Bsp143I GATC 6 cut(s) 98, 434, 535, 608, 709, 737
BspACI CCGC 6 cut(s) 167, 342, 495, 557, 842, 912
BspANI GGCC 1 cut(s) 295
BspCNI CTCAG 2 cut(s) 351, 385
BspFNI CGCG 2 cut(s) 167, 495
BspHI TCATGA 1 cut(s) 466
BspLI GGNNCC 3 cut(s) 739, 978, 1023
BspPI GGATC 5 cut(s) 106, 442, 704, 732, 745
BspQI GCTCTTC 1 cut(s) 615
BspTNI GGTCTC 1 cut(s) 929
BsrDI GCAATG 1 cut(s) 436
BsrFI RCCGGY 1 cut(s) 973
BsrI ACTGG 2 cut(s) 729, 994
BssAI RCCGGY 1 cut(s) 973
BssECI CCNNGG 2 cut(s) 33, 918
BssMI GATC 6 cut(s) 98, 434, 535, 608, 709, 737
BssNI GRCGYC 1 cut(s) 12
Bst2UI CCWGG 2 cut(s) 297, 919
Bst4CI ACNGT 2 cut(s) 203, 681
Bst6I CTCTTC 2 cut(s) 615, 681
BstACI GRCGYC 1 cut(s) 12
BstC8I GCNNGC 2 cut(s) 93, 293
BstDEI CTNAG 2 cut(s) 359, 372
BstF5I GGATG 4 cut(s) 586, 811, 908, 928
BstFNI CGCG 2 cut(s) 167, 495
BstHHI GCGC 1 cut(s) 167
BstKTI GATC 6 cut(s) 101, 437, 538, 611, 712, 740
BstMAI GTCTC 4 cut(s) 3, 375, 750, 929
BstMBI GATC 6 cut(s) 98, 434, 535, 608, 709, 737
BstMWI GCNNNNNNNGC 3 cut(s) 297, 342, 559
BstNI CCWGG 2 cut(s) 297, 919
BstSCI CCNGG 6 cut(s) 20, 32, 33, 295, 479, 917
BstUI CGCG 2 cut(s) 167, 495
BstV1I GCAGC 4 cut(s) 413, 565, 571, 655
BstX2I RGATCY 3 cut(s) 434, 709, 737
BstYI RGATCY 3 cut(s) 434, 709, 737
BsuI GTATCC 1 cut(s) 891
BsuRI GGCC 1 cut(s) 295
BtsCI GGATG 4 cut(s) 586, 811, 908, 928
BtsIMutI CAGTG 2 cut(s) 160, 722
Cac8I GCNNGC 2 cut(s) 93, 293
CciI TCATGA 1 cut(s) 466
CfoI GCGC 1 cut(s) 167
Cfr10I RCCGGY 1 cut(s) 973
Cfr13I GGNCC 1 cut(s) 203
Cfr9I CCCGGG 1 cut(s) 33
CseI GACGC 2 cut(s) 20, 47
Csp6I GTAC 1 cut(s) 309
CviAII CATG 4 cut(s) 184, 467, 485, 793
CviQI GTAC 1 cut(s) 309
DdeI CTNAG 2 cut(s) 359, 372
DpnI GATC 6 cut(s) 100, 436, 537, 610, 711, 739
DpnII GATC 6 cut(s) 98, 434, 535, 608, 709, 737
Eam1104I CTCTTC 2 cut(s) 615, 681
EarI CTCTTC 2 cut(s) 615, 681
EciI GGCGGA 1 cut(s) 901
Eco31I GGTCTC 1 cut(s) 929
Eco47I GGWCC 1 cut(s) 203
Eco57I CTGAAG 1 cut(s) 339
Eco88I CYCGRG 1 cut(s) 33
EcoRI GAATTC 1 cut(s) 646
EcoRII CCWGG 2 cut(s) 295, 917
Esp3I CGTCTC 2 cut(s) 3, 375
FaeI CATG 4 cut(s) 187, 470, 488, 796
FaiI YATR 9 cut(s) 30, 185, 222, 462, 468, 486, 749, 794, 905
FaqI GGGAC 1 cut(s) 50
FatI CATG 4 cut(s) 183, 466, 484, 792
FbaI TGATCA 1 cut(s) 535
Fnu4HI GCNGC 6 cut(s) 343, 427, 554, 557, 560, 669
FokI GGATG 4 cut(s) 593, 818, 895, 935
Fsp4HI GCNGC 6 cut(s) 343, 427, 554, 557, 560, 669
FspBI CTAG 1 cut(s) 597
GlaI GCGC 1 cut(s) 166
GluI GCNGC 6 cut(s) 343, 427, 554, 557, 560, 669
GsuI CTGGAG 2 cut(s) 746, 963
HaeIII GGCC 1 cut(s) 295
HapII CCGG 6 cut(s) 21, 34, 480, 651, 939, 974
HgaI GACGC 2 cut(s) 20, 47
HhaI GCGC 1 cut(s) 167
Hin1I GRCGYC 1 cut(s) 12
Hin1II CATG 4 cut(s) 187, 470, 488, 796
Hin6I GCGC 1 cut(s) 165
HinP1I GCGC 1 cut(s) 165
HindIII AAGCTT 1 cut(s) 89
HinfI GANTC 4 cut(s) 25, 109, 115, 926
HpaII CCGG 6 cut(s) 21, 34, 480, 651, 939, 974
HphI GGTGA 1 cut(s) 158
Hpy166II GTNNAC 3 cut(s) 206, 217, 589
Hpy188I TCNGA 9 cut(s) 108, 114, 270, 306, 319, 375, 445, 816, 862
Hpy188III TCNNGA 2 cut(s) 467, 871
Hpy8I GTNNAC 3 cut(s) 206, 217, 589
HpyAV CCTTC 5 cut(s) 65, 241, 363, 745, 1035
HpyCH4III ACNGT 2 cut(s) 203, 681
HpyCH4V TGCA 4 cut(s) 95, 429, 632, 656
HpyF10VI GCNNNNNNNGC 3 cut(s) 297, 342, 559
HpyF3I CTNAG 2 cut(s) 359, 372
Hsp92I GRCGYC 1 cut(s) 12
Hsp92II CATG 4 cut(s) 187, 470, 488, 796
HspAI GCGC 1 cut(s) 165
Ksp22I TGATCA 1 cut(s) 535
Kzo9I GATC 6 cut(s) 98, 434, 535, 608, 709, 737
LguI GCTCTTC 1 cut(s) 615
LmnI GCTCC 2 cut(s) 826, 982
Lsp1109I GCAGC 4 cut(s) 413, 565, 571, 655
LweI GCATC 5 cut(s) 177, 796, 829, 841, 917
MaeI CTAG 1 cut(s) 597
MaeIII GTNAC 2 cut(s) 74, 348
MalI GATC 6 cut(s) 100, 436, 537, 610, 711, 739
MboI GATC 6 cut(s) 98, 434, 535, 608, 709, 737
MflI RGATCY 3 cut(s) 434, 709, 737
MhlI GDGCHC 1 cut(s) 87
MluCI AATT 5 cut(s) 210, 640, 646, 662, 772
MlyI GAGTC 2 cut(s) 103, 920
MmeI TCCRAC 3 cut(s) 308, 663, 999
MroXI GAANNNNTTC 1 cut(s) 176
MseI TTAA 2 cut(s) 549, 570
MspI CCGG 6 cut(s) 21, 34, 480, 651, 939, 974
MspR9I CCNGG 6 cut(s) 22, 34, 35, 297, 481, 919
Mva1269I GAATGC 2 cut(s) 481, 632
MvaI CCWGG 2 cut(s) 297, 919
MvnI CGCG 2 cut(s) 167, 495
MwoI GCNNNNNNNGC 3 cut(s) 297, 342, 559
NciI CCSGG 4 cut(s) 22, 34, 35, 481
NdeII GATC 6 cut(s) 98, 434, 535, 608, 709, 737
NlaIII CATG 4 cut(s) 187, 470, 488, 796
NlaIV GGNNCC 3 cut(s) 739, 978, 1023
PagI TCATGA 1 cut(s) 466
PciSI GCTCTTC 1 cut(s) 615
PctI GAATGC 2 cut(s) 481, 632
PdmI GAANNNNTTC 1 cut(s) 176
PfeI GAWTC 2 cut(s) 25, 115
PkrI GCNGC 6 cut(s) 344, 428, 555, 558, 561, 670
PleI GAGTC 2 cut(s) 103, 920
PpsI GAGTC 2 cut(s) 103, 920
Psp6I CCWGG 2 cut(s) 295, 917
PspGI CCWGG 2 cut(s) 295, 917
PspN4I GGNNCC 3 cut(s) 739, 978, 1023
PspPI GGNCC 1 cut(s) 203
PsuI RGATCY 3 cut(s) 434, 709, 737
RsaI GTAC 1 cut(s) 310
RsaNI GTAC 1 cut(s) 309
SapI GCTCTTC 1 cut(s) 615
SaqAI TTAA 2 cut(s) 549, 570
SatI GCNGC 6 cut(s) 343, 427, 554, 557, 560, 669
Sau3AI GATC 6 cut(s) 98, 434, 535, 608, 709, 737
Sau96I GGNCC 1 cut(s) 203
ScaI AGTACT 1 cut(s) 310
SchI GAGTC 2 cut(s) 103, 920
ScrFI CCNGG 6 cut(s) 22, 34, 35, 297, 481, 919
SduI GDGCHC 1 cut(s) 87
SfaNI GCATC 5 cut(s) 177, 796, 829, 841, 917
SinI GGWCC 1 cut(s) 203
SmaI CCCGGG 1 cut(s) 35
SmlI CTYRAG 2 cut(s) 86, 928
SmoI CTYRAG 2 cut(s) 86, 928
Sse9I AATT 5 cut(s) 210, 640, 646, 662, 772
SsiI CCGC 6 cut(s) 167, 342, 495, 557, 842, 912
SspI AATATT 2 cut(s) 176, 532
SspMI CTAG 1 cut(s) 597
StyD4I CCNGG 6 cut(s) 20, 32, 33, 295, 479, 917
TaaI ACNGT 2 cut(s) 203, 681
TaqI TCGA 2 cut(s) 870, 967
TasI AATT 5 cut(s) 210, 640, 646, 662, 772
TatI WGTACW 1 cut(s) 308
TauI GCSGC 2 cut(s) 345, 559
TfiI GAWTC 2 cut(s) 25, 115
Tru1I TTAA 2 cut(s) 549, 570
Tru9I TTAA 2 cut(s) 549, 570
TscAI CASTG 2 cut(s) 160, 729
TseI GCWGC 4 cut(s) 426, 553, 559, 668
TspDTI ATGAA 7 cut(s) 17, 455, 488, 713, 764, 845, 849
TspMI CCCGGG 1 cut(s) 33
TspRI CASTG 2 cut(s) 160, 729
VpaK11BI GGWCC 1 cut(s) 203
XapI RAATTY 3 cut(s) 640, 646, 772
XmaI CCCGGG 1 cut(s) 33
XmnI GAANNNNTTC 1 cut(s) 176
XspI CTAG 1 cut(s) 597
ZrmI AGTACT 1 cut(s) 310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.