MD06G1195900.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0017728
Physical Location & Seq
Reverse (-)
32983205 .. 32984572
1368 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1195900.v1.1.491

Sequence Viewer

Length: 1368 bp
ATGCACGATGCCATATTTCAAAAGGTGCACAGGGTTATGTCCCTTTTCAAGCAATGCACCACCACGAAACATGTAAAGCAAATCCATGGGCACATAATCCACAAAGGGCTTGATCAAAACGTCTTTGTTCTTGGCAAGATCGTCTCTTTCTGCGCGGTTTCGGACCGCGGAGACATGGACTATGCCGTCTCTGTTTTCAGAAGCGTTGAAAGCCCGGATGGGTTTTTGTGGAACACAATGATCAGAGGGTTTGGGAAGACCAGGAAGCCTGAAAGGGCATTTGAGTTTTATAAGAGAATGCAGGAGAATGGAAAGGTGGCTGATAATTTCACACTTTCCTTCTTGCTCAAGGTTTGTGGGCAATTGGGTTCTTACGTTTTGGGGAAACAGATGCATTGTGCTGCTCTGAAGCATGGGTTGGAGTCTCATGTCTTTGTAAGGAACACTCTCATTCATATGTATGGGATGTTGAAGGATGACCAAACTGCGTTCAATCTGTTTGATGAAATGCCTCAACCAGATTTAGTGGCTTGGAATACTATCATTGATAGTTATGTCAATTGCGGAAAGTGTAAGGAAGCACTTGACTTGTTTTTACAGATGGTAAACAGTGGCACAGAACCTGATGACGCCACGGTGGTTTTGACCCTCTCGGCGTGCTCTACATTGGGTGCATTAGATTTTGGGAGGAGAGTTCATTCTTTTATTAATCGTTTGAATCTTAGCAGCAGTGTGACGGTGACTAATTCGCTTATCGGCATGTATGCAAAGTGCGGAGCTTTTGAAGAAGCATATGAGACGTTTTGTAAGATGAAGGGGAAGAACATAGTGTCGTGGAACACGATGATTCTGGGGCTTGCAACGCATGGGCATGCAGACGATGCATTGGAATTGTTCTCGAAGATGTTGAAGGAAAAGCTTGGGATGCCGGATGAAGTAACTTTCTTGGGAGTTTTGTGTGCTTGTAGCCATGGAGGGATGGTTGATGAAGGAAGAAGGTATTTTGATGTTATGAGCAAGGAGTATCGTATCCAACCAACCATGAAGCACTACGGATGCATGGTGGATATGTTGGGGCGAGCCGGGTACGTGGAGGAGGCTTACAGGTTGATAAAAAACATGCCGATGGAGTGTAATGCCGTTACATGGAGGACATTGTTGGGAGCGTGTCGGCTGCACGGTGATATTGTGCTCGGAGAAAAGGTGAGGGGTCATCTTTTGGAGCTGGAACCAGATCACAGCAGCGACTACGTTCTTCTATCGAACATGTACGCAAGTTCAGGCGAATGGAACGAAGTTATACGAGTGAGAAAATCAATGATTGACAGGGGAGTTCAAAAACCAGAGCCTGGTAATAGCCTGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

456

Amino Acids

51.21

Weight (kDa)

7.25

Isoelectric Point (pI)

36.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 72 - 119 1.6e-09 PPR repeat family
PPR PF01535 76 - 104 9.1e-08 PPR repeat
PPR_2 PF13041 173 - 220 1.5e-11 PPR repeat family
PPR PF01535 176 - 205 2.1e-07 PPR repeat
PPR_2 PF13041 274 - 323 7.4e-10 PPR repeat family
PPR PF01535 277 - 305 1.6e-06 PPR repeat
E_motif PF20431 393 - 454 1.3e-14 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017728)

Species Orthologous Gene IDs
malus_domestica MD06G1195900.v1.1
prunus_persica Prupe.5G195700_v2.0.a1
pyrus_communis pycom06g17310
rosa_chinensis RchiOBHm_Chr7g0181731
rosa_laevigata RLG00000005173
rosa_multiflora Rmu_sc0000988.1_g000008
rosa_roxburghii Rroxscaffold_3G00271770
rosa_rugosa Rorug06G0443000
rosa_samantha Rh7AG045600 Rh7BG044800 Rh7CG046300
rosa_wichuraiana Rw7G003800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 291
AasI GACNNNNNNGTC 1 cut(s) 185
AccB7I CCANNNNNTGG 1 cut(s) 1349
AccII CGCG 2 cut(s) 155, 168
AciI CCGC 5 cut(s) 155, 166, 168, 564, 774
AcuI CTGAAG 1 cut(s) 428
AcyI GRCGYC 1 cut(s) 630
AfaI GTAC 2 cut(s) 1088, 1271
AfiI CCNNNNNNNGG 2 cut(s) 1146, 1349
AflIII ACRYGT 2 cut(s) 70, 1266
AgsI TTSAA 9 cut(s) 20, 49, 209, 472, 493, 718, 785, 910, 1337
AjnI CCWGG 3 cut(s) 260, 1348, 1359
AjuI GAANNNNNNNTTGG 2 cut(s) 401, 433
AluBI AGCT 3 cut(s) 779, 919, 1225
AluI AGCT 3 cut(s) 779, 919, 1225
Alw21I GWGCWC 3 cut(s) 30, 662, 1194
Alw26I GTCTC 5 cut(s) 148, 165, 193, 429, 791
Alw44I GTGCAC 1 cut(s) 26
AlwNI CAGNNNCTG 2 cut(s) 623, 1349
ApaLI GTGCAC 1 cut(s) 26
ApeKI GCWGC 4 cut(s) 401, 726, 1174, 1242
AseI ATTAAT 1 cut(s) 708
AspLEI GCGC 1 cut(s) 155
AspS9I GGNCC 1 cut(s) 163
AsuC2I CCSGG 2 cut(s) 215, 1084
AsuHPI GGTGA 3 cut(s) 751, 1193, 1216
AvaII GGWCC 1 cut(s) 163
BaeGI GKGCMC 2 cut(s) 30, 93
BbsI GAAGAC 1 cut(s) 263
Bbv12I GWGCWC 3 cut(s) 30, 662, 1194
BbvI GCAGC 4 cut(s) 388, 738, 1161, 1254
BccI CCATC 4 cut(s) 212, 595, 973, 1120
BceAI ACGGC 2 cut(s) 170, 1124
BciT130I CCWGG 3 cut(s) 262, 1350, 1361
BciVI GTATCC 1 cut(s) 1040
BclI TGATCA 2 cut(s) 112, 240
BcnI CCSGG 2 cut(s) 215, 1084
BcoDI GTCTC 5 cut(s) 148, 165, 193, 429, 791
BfuI GTATCC 1 cut(s) 1040
BisI GCNGC 4 cut(s) 402, 727, 1175, 1243
BlsI GCNGC 4 cut(s) 403, 728, 1176, 1244
Bme1390I CCNGG 5 cut(s) 215, 262, 1084, 1350, 1361
Bme18I GGWCC 1 cut(s) 163
BmgT120I GGNCC 1 cut(s) 163
BmiI GGNNCC 1 cut(s) 1230
BmrFI CCNGG 5 cut(s) 215, 262, 1084, 1350, 1361
BmsI GCATC 4 cut(s) 381, 871, 915, 1046
BpiI GAAGAC 1 cut(s) 263
BpuEI CTTGAG 1 cut(s) 332
BpuMI CCSGG 2 cut(s) 215, 1084
BsaAI YACGTR 1 cut(s) 1090
BsaHI GRCGYC 1 cut(s) 630
BsaJI CCNNGG 4 cut(s) 85, 166, 633, 970
BsaXI ACNNNNNCTCC 4 cut(s) 966, 996, 1188, 1218
Bsc4I CCNNNNNNNGG 2 cut(s) 1146, 1349
Bse3DI GCAATG 1 cut(s) 59
BseBI CCWGG 3 cut(s) 262, 1350, 1361
BseDI CCNNGG 4 cut(s) 85, 166, 633, 970
BseGI GGATG 7 cut(s) 223, 471, 481, 930, 937, 984, 1061
BseLI CCNNNNNNNGG 2 cut(s) 1146, 1349
BseMI GCAATG 1 cut(s) 59
BseRI GAGGAG 2 cut(s) 703, 1109
BseSI GKGCMC 2 cut(s) 30, 93
BseXI GCAGC 4 cut(s) 388, 738, 1161, 1254
BsgI GTGCAG 1 cut(s) 1160
Bsh1236I CGCG 2 cut(s) 155, 168
BsiHKAI GWGCWC 3 cut(s) 30, 662, 1194
BsiSI CCGG 3 cut(s) 215, 929, 1083
BslFI GGGAC 1 cut(s) 25
BslI CCNNNNNNNGG 2 cut(s) 1146, 1349
BsmAI GTCTC 5 cut(s) 148, 165, 193, 429, 791
BsmBI CGTCTC 3 cut(s) 148, 193, 791
BsmFI GGGAC 1 cut(s) 25
BsmI GAATGC 1 cut(s) 303
Bsp1286I GDGCHC 4 cut(s) 30, 93, 662, 1194
Bsp143I GATC 4 cut(s) 112, 138, 240, 1234
Bsp19I CCATGG 2 cut(s) 85, 970
BspACI CCGC 5 cut(s) 155, 166, 168, 564, 774
BspFNI CGCG 2 cut(s) 155, 168
BspLI GGNNCC 1 cut(s) 1230
BsrDI GCAATG 1 cut(s) 59
BssECI CCNNGG 4 cut(s) 85, 166, 633, 970
BssMI GATC 4 cut(s) 112, 138, 240, 1234
BssNI GRCGYC 1 cut(s) 630
BssT1I CCWWGG 2 cut(s) 85, 970
Bst2UI CCWGG 3 cut(s) 262, 1350, 1361
Bst4CI ACNGT 4 cut(s) 611, 637, 739, 1181
BstACI GRCGYC 1 cut(s) 630
BstAPI GCANNNNNTGC 1 cut(s) 881
BstBAI YACGTR 1 cut(s) 1090
BstC8I GCNNGC 4 cut(s) 658, 858, 873, 1080
BstDEI CTNAG 1 cut(s) 722
BstDSI CCRYGG 4 cut(s) 85, 166, 633, 970
BstF5I GGATG 7 cut(s) 223, 471, 481, 930, 937, 984, 1061
BstFNI CGCG 2 cut(s) 155, 168
BstHHI GCGC 1 cut(s) 155
BstKTI GATC 4 cut(s) 115, 141, 243, 1237
BstMAI GTCTC 5 cut(s) 148, 165, 193, 429, 791
BstMBI GATC 4 cut(s) 112, 138, 240, 1234
BstMWI GCNNNNNNNGC 4 cut(s) 210, 862, 881, 925
BstNI CCWGG 3 cut(s) 262, 1350, 1361
BstNSI RCATGY 5 cut(s) 74, 763, 875, 1123, 1270
BstSCI CCNGG 5 cut(s) 213, 260, 1082, 1348, 1359
BstSLI GKGCMC 2 cut(s) 30, 93
BstUI CGCG 2 cut(s) 155, 168
BstV1I GCAGC 4 cut(s) 388, 738, 1161, 1254
BstV2I GAAGAC 1 cut(s) 263
BsuI GTATCC 1 cut(s) 1040
BtgI CCRYGG 4 cut(s) 85, 166, 633, 970
BtsCI GGATG 7 cut(s) 223, 471, 481, 930, 937, 984, 1061
BtsI GCAGTG 1 cut(s) 736
BtsIMutI CAGTG 2 cut(s) 616, 736
Cac8I GCNNGC 4 cut(s) 658, 858, 873, 1080
CaiI CAGNNNCTG 2 cut(s) 623, 1349
CfoI GCGC 1 cut(s) 155
Cfr13I GGNCC 1 cut(s) 163
Cfr42I CCGCGG 1 cut(s) 169
CpoI CGGWCCG 1 cut(s) 163
CseI GACGC 1 cut(s) 638
Csp6I GTAC 2 cut(s) 1087, 1270
CspCI CAANNNNNGTGG 2 cut(s) 337, 372
CspI CGGWCCG 1 cut(s) 163
CviQI GTAC 2 cut(s) 1087, 1270
DdeI CTNAG 1 cut(s) 722
DpnI GATC 4 cut(s) 114, 140, 242, 1236
DpnII GATC 4 cut(s) 112, 138, 240, 1234
DrdI GACNNNNNNGTC 1 cut(s) 185
DseDI GACNNNNNNGTC 1 cut(s) 185
Eco130I CCWWGG 2 cut(s) 85, 970
Eco47I GGWCC 1 cut(s) 163
Eco57I CTGAAG 1 cut(s) 428
EcoRII CCWGG 3 cut(s) 260, 1348, 1359
EcoT14I CCWWGG 2 cut(s) 85, 970
EcoT22I ATGCAT 3 cut(s) 396, 886, 1061
ErhI CCWWGG 2 cut(s) 85, 970
Esp3I CGTCTC 3 cut(s) 148, 193, 791
FaqI GGGAC 1 cut(s) 25
FauNDI CATATG 2 cut(s) 456, 793
FbaI TGATCA 2 cut(s) 112, 240
Fnu4HI GCNGC 4 cut(s) 402, 727, 1175, 1243
FokI GGATG 7 cut(s) 230, 478, 488, 937, 944, 991, 1068
Fsp4HI GCNGC 4 cut(s) 402, 727, 1175, 1243
GlaI GCGC 1 cut(s) 154
GluI GCNGC 4 cut(s) 402, 727, 1175, 1243
HapII CCGG 3 cut(s) 215, 929, 1083
HgaI GACGC 1 cut(s) 638
HhaI GCGC 1 cut(s) 155
Hin1I GRCGYC 1 cut(s) 630
Hin6I GCGC 1 cut(s) 153
HinP1I GCGC 1 cut(s) 153
HindIII AAGCTT 1 cut(s) 917
HinfI GANTC 3 cut(s) 422, 718, 847
HpaII CCGG 3 cut(s) 215, 929, 1083
HphI GGTGA 3 cut(s) 751, 1193, 1216
Hpy166II GTNNAC 2 cut(s) 28, 607
Hpy188I TCNGA 5 cut(s) 163, 200, 245, 408, 1196
Hpy188III TCNNGA 1 cut(s) 898
Hpy8I GTNNAC 2 cut(s) 28, 607
HpyAV CCTTC 6 cut(s) 349, 466, 808, 904, 983, 990
HpyCH4III ACNGT 4 cut(s) 611, 637, 739, 1181
HpyCH4IV ACGT 5 cut(s) 120, 375, 800, 1089, 1251
HpyF10VI GCNNNNNNNGC 4 cut(s) 210, 862, 881, 925
HpyF3I CTNAG 1 cut(s) 722
HpySE526I ACGT 5 cut(s) 120, 375, 800, 1089, 1251
Hsp92I GRCGYC 1 cut(s) 630
HspAI GCGC 1 cut(s) 153
Ksp22I TGATCA 2 cut(s) 112, 240
KspI CCGCGG 1 cut(s) 169
Kzo9I GATC 4 cut(s) 112, 138, 240, 1234
LmnI GCTCC 3 cut(s) 776, 1163, 1222
Lsp1109I GCAGC 4 cut(s) 388, 738, 1161, 1254
LweI GCATC 4 cut(s) 381, 871, 915, 1046
MaeII ACGT 5 cut(s) 120, 375, 800, 1089, 1251
MaeIII GTNAC 4 cut(s) 733, 739, 937, 1141
MalI GATC 4 cut(s) 114, 140, 242, 1236
MboI GATC 4 cut(s) 112, 138, 240, 1234
MboII GAAGA 6 cut(s) 268, 797, 832, 913, 1005, 1247
MfeI CAATTG 2 cut(s) 362, 559
MhlI GDGCHC 4 cut(s) 30, 93, 662, 1194
MluCI AATT 5 cut(s) 325, 362, 559, 745, 890
MlyI GAGTC 1 cut(s) 431
MmeI TCCRAC 2 cut(s) 399, 1057
MnlI CCTC 9 cut(s) 239, 522, 659, 681, 968, 1087, 1090, 1143, 1200
Mph1103I ATGCAT 3 cut(s) 396, 886, 1061
MseI TTAA 2 cut(s) 708, 1366
MslI CAYNNNNRTG 4 cut(s) 455, 459, 870, 1124
MspA1I CMGCKG 1 cut(s) 168
MspI CCGG 3 cut(s) 215, 929, 1083
MspR9I CCNGG 5 cut(s) 215, 262, 1084, 1350, 1361
MunI CAATTG 2 cut(s) 362, 559
Mva1269I GAATGC 1 cut(s) 303
MvaI CCWGG 3 cut(s) 262, 1350, 1361
MvnI CGCG 2 cut(s) 155, 168
MwoI GCNNNNNNNGC 4 cut(s) 210, 862, 881, 925
NciI CCSGG 2 cut(s) 215, 1084
NcoI CCATGG 2 cut(s) 85, 970
NdeI CATATG 2 cut(s) 456, 793
NdeII GATC 4 cut(s) 112, 138, 240, 1234
NlaIV GGNNCC 1 cut(s) 1230
NmeAIII GCCGAG 1 cut(s) 632
NmuCI GTSAC 2 cut(s) 733, 739
NsiI ATGCAT 3 cut(s) 396, 886, 1061
NspI RCATGY 5 cut(s) 74, 763, 875, 1123, 1270
PaeI GCATGC 1 cut(s) 875
PciI ACATGT 2 cut(s) 70, 1266
PcsI WCGNNNNNNNCGW 1 cut(s) 839
PctI GAATGC 1 cut(s) 303
PfeI GAWTC 2 cut(s) 718, 847
PflMI CCANNNNNTGG 1 cut(s) 1349
PkrI GCNGC 4 cut(s) 403, 728, 1176, 1244
PleI GAGTC 1 cut(s) 430
PpsI GAGTC 1 cut(s) 430
Ppu21I YACGTR 1 cut(s) 1090
PscI ACATGT 2 cut(s) 70, 1266
PshBI ATTAAT 1 cut(s) 708
PsiI TTATAA 1 cut(s) 291
Psp6I CCWGG 3 cut(s) 260, 1348, 1359
PspGI CCWGG 3 cut(s) 260, 1348, 1359
PspN4I GGNNCC 1 cut(s) 1230
PspPI GGNCC 1 cut(s) 163
PstNI CAGNNNCTG 2 cut(s) 623, 1349
RsaI GTAC 2 cut(s) 1088, 1271
RsaNI GTAC 2 cut(s) 1087, 1270
RseI CAYNNNNRTG 4 cut(s) 455, 459, 870, 1124
Rsr2I CGGWCCG 1 cut(s) 163
RsrII CGGWCCG 1 cut(s) 163
SacII CCGCGG 1 cut(s) 169
SaqAI TTAA 2 cut(s) 708, 1366
SatI GCNGC 4 cut(s) 402, 727, 1175, 1243
Sau3AI GATC 4 cut(s) 112, 138, 240, 1234
Sau96I GGNCC 1 cut(s) 163
SchI GAGTC 1 cut(s) 431
ScrFI CCNGG 5 cut(s) 215, 262, 1084, 1350, 1361
SduI GDGCHC 4 cut(s) 30, 93, 662, 1194
SfaNI GCATC 4 cut(s) 381, 871, 915, 1046
Sfr303I CCGCGG 1 cut(s) 169
SgrBI CCGCGG 1 cut(s) 169
SinI GGWCC 1 cut(s) 163
SmiMI CAYNNNNRTG 4 cut(s) 455, 459, 870, 1124
SmlI CTYRAG 1 cut(s) 347
SmoI CTYRAG 1 cut(s) 347
SphI GCATGC 1 cut(s) 875
Sse9I AATT 5 cut(s) 325, 362, 559, 745, 890
SsiI CCGC 5 cut(s) 155, 166, 168, 564, 774
StyD4I CCNGG 5 cut(s) 213, 260, 1082, 1348, 1359
StyI CCWWGG 2 cut(s) 85, 970
TaaI ACNGT 4 cut(s) 611, 637, 739, 1181
TaiI ACGT 5 cut(s) 123, 378, 803, 1092, 1254
TaqI TCGA 2 cut(s) 899, 1262
TasI AATT 5 cut(s) 325, 362, 559, 745, 890
TfiI GAWTC 2 cut(s) 718, 847
Tru1I TTAA 2 cut(s) 708, 1366
Tru9I TTAA 2 cut(s) 708, 1366
TscAI CASTG 2 cut(s) 616, 736
TseFI GTSAC 2 cut(s) 733, 739
TseI GCWGC 4 cut(s) 401, 726, 1174, 1242
Tsp45I GTSAC 2 cut(s) 733, 739
TspDTI ATGAA 7 cut(s) 443, 519, 686, 827, 948, 1002, 1058
TspGWI ACGGA 1 cut(s) 1068
TspRI CASTG 2 cut(s) 616, 736
Van91I CCANNNNNTGG 1 cut(s) 1349
VneI GTGCAC 1 cut(s) 26
VpaK11BI GGWCC 1 cut(s) 163
VspI ATTAAT 1 cut(s) 708
XceI RCATGY 5 cut(s) 74, 763, 875, 1123, 1270
Zsp2I ATGCAT 3 cut(s) 396, 886, 1061
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.