RchiOBHm_Chr7g0181731

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0017728
Physical Location & Seq
Reverse (-)
3251551 .. 3253130
1580 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ16209

Sequence Viewer

Length: 1506 bp
ATGCTCCACCTCTCTTCTTCTTCTTCTTCTTCTTCCTTTCCCACCAAGCCAACAATGCGCAGCAACATATTTCACAAGGCACAAAGGGTCATGACCCTTTTCAAGCAGTGCACCACCATGAAAGAGGTCAAGCAACTCCATGCCCATATAACCCACACCGGGCTCGATCAAACCCTCTGTGTTCTCGGCAAGGTCATCTCATTTTGCGCGGTTTCGGACCTCGGAGACATGGACTATGCGGTCTCTGTGTTCAGCAGCATTGAAAGCCCAGATGGGTTTTTATGGAACACCATGATTAGAGGGTTTGGGAAGACTGATAAGCCTGAAAGGGCGTTTGAGTTTTGTAAGAGAATGCAGGAGAAAGGAAATGTAGCTGACAATTTTACCCTCTCGTTCTTGCTCAAGGTTTCTGGGCAATTGGGATCGGATATATTGGGGAAGCAGATACATTGTACTACTGTGAAAAATGGGTTTGATTCTAATGTGTTTGTGAGGAACACTCTAATCCATATGTATGGTATGTTTGGGGATGCAAAAACTGCTCTCCACCTGTTCGATGAAATGCCTAGCCCAGATTTAGTGGCGTGGAATACCATTATTGATAGTCATGTGAATTGCGGAAAGTGCAACGAAGCGCTAGATTTGTTCTTGAGGATGATGGATAGGGGTGTAGATACTGACGAGGCCACTGTCGTTGTGACCCTCTCGGCATGTTCTACATTGGGTGCTTTAGATTTTGGGAGGTGGGTACATTGTTTGGTTGATCATACGGATCTTGGCAACGTTGTGACGGTGTCTAATTCGTTAATTGACATGTATGCGAAGTGTGGAGCTGTTGAAGAAGCGTATGATGCTTTTAATAAGATGAAGGGGAAGAAGAACATAGTATCTTGGAACACGATGATTCTCGGGCTTGCGACGCATGGTCATGCGGACAAATCTTTGGACCTGTTCTTGAAAATGTTGGAGGAGAAGCTTGAGCGACCGGATGGTGTTACTTTCTTGGGAGTTCTGTGTGCTTGTAGTTATGGAGGGATGGTTGATGAAGGAAGAAGATATTTTGAAATTATGAGCAAAGACTATCAGATTCAACCAACAGTGAAGCACTACGGATGCATGGTTGATATGTTGGGGCGAGCTGGGTTTGTGGAAGAGGCTTATAGGTTGATAAGGAGCATGCCGGTGAAGTGTAATGCCATTGTATGGAGGACATTGTTGTCGGCGTGTCAGGTGCACGGTGAGGTCGAGCTTGGAGAGAAGGTCAGGAGTCATCTGTTGGAGTTGGCTTCAGATCATAGCAGCGATTATGTGCTTCTGGCTAACATGTATGCAAGTTTAGGCCAGTGGAACGAAGCTATGAGAGTAAGAAGGTCAATGCAGTACAGGGGAGTTGAGAAACCTGAGCCCGGTAATAGCTTGGTTGGCGCAGATCTTCATATGAAGTTGAAAATGGACTCAACTGAAGCCTATTCTGAAGATATAAATGCAGCAGTTACTAGTTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

501

Amino Acids

55.77

Weight (kDa)

6.14

Isoelectric Point (pI)

40.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 90 - 135 5.2e-08 PPR repeat family
PPR PF01535 94 - 122 6.1e-07 PPR repeat
PPR_2 PF13041 191 - 239 6e-10 PPR repeat family
PPR PF01535 194 - 224 8.8e-07 PPR repeat
PPR_2 PF13041 293 - 341 1.6e-06 PPR repeat family
E_motif PF20431 412 - 474 1.9e-14 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017728)

Species Orthologous Gene IDs
malus_domestica MD06G1195900.v1.1
prunus_persica Prupe.5G195700_v2.0.a1
pyrus_communis pycom06g17310
rosa_chinensis RchiOBHm_Chr7g0181731
rosa_laevigata RLG00000005173
rosa_multiflora Rmu_sc0000988.1_g000008
rosa_roxburghii Rroxscaffold_3G00271770
rosa_rugosa Rorug06G0443000
rosa_samantha Rh7AG045600 Rh7BG044800 Rh7CG046300
rosa_wichuraiana Rw7G003800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 239, 1216
Acc16I TGCGCA 1 cut(s) 59
AccB7I CCANNNNNTGG 1 cut(s) 1203
AccII CGCG 1 cut(s) 209
AciI CCGC 4 cut(s) 209, 239, 618, 932
AclI AACGTT 1 cut(s) 783
AclWI GGATC 2 cut(s) 430, 780
AcuI CTGAAG 3 cut(s) 1272, 1482, 1494
AfaI GTAC 3 cut(s) 454, 750, 1382
AfeI AGCGCT 1 cut(s) 636
AfiI CCNNNNNNNGG 3 cut(s) 159, 1203, 1406
AflIII ACRYGT 2 cut(s) 813, 1323
AgsI TTSAA 7 cut(s) 103, 263, 839, 958, 1064, 1091, 1447
AhdI GACNNNNNGTC 1 cut(s) 924
AhlI ACTAGT 1 cut(s) 1496
AluBI AGCT 7 cut(s) 374, 833, 976, 1139, 1249, 1355, 1416
AluI AGCT 7 cut(s) 374, 833, 976, 1139, 1249, 1355, 1416
Alw21I GWGCWC 2 cut(s) 113, 1236
Alw26I GTCTC 2 cut(s) 219, 247
Alw44I GTGCAC 2 cut(s) 109, 1232
AlwI GGATC 2 cut(s) 430, 780
Ama87I CYCGRG 1 cut(s) 908
Aor51HI AGCGCT 1 cut(s) 636
AoxI GGCC 2 cut(s) 684, 1339
ApaLI GTGCAC 2 cut(s) 109, 1232
ApeKI GCWGC 4 cut(s) 60, 255, 1299, 1487
AspLEI GCGC 4 cut(s) 60, 209, 637, 1427
AspS9I GGNCC 2 cut(s) 217, 946
AsuC2I CCSGG 2 cut(s) 160, 1407
AsuHPI GGTGA 2 cut(s) 1195, 1250
AvaI CYCGRG 1 cut(s) 908
AvaII GGWCC 2 cut(s) 217, 946
BaeGI GKGCMC 2 cut(s) 113, 1236
BanII GRGCYC 2 cut(s) 165, 1407
BbsI GAAGAC 1 cut(s) 317
Bbv12I GWGCWC 2 cut(s) 113, 1236
BbvI GCAGC 4 cut(s) 72, 267, 1311, 1499
BccI CCATC 4 cut(s) 266, 652, 983, 1030
BclI TGATCA 1 cut(s) 763
BcnI CCSGG 2 cut(s) 160, 1407
BcoDI GTCTC 2 cut(s) 219, 247
BcuI ACTAGT 1 cut(s) 1496
BfaI CTAG 3 cut(s) 567, 638, 1497
BfoI RGCGCY 1 cut(s) 638
BglII AGATCT 1 cut(s) 1429
BisI GCNGC 4 cut(s) 61, 256, 1300, 1488
BlsI GCNGC 4 cut(s) 62, 257, 1301, 1489
Bme1390I CCNGG 2 cut(s) 160, 1407
Bme18I GGWCC 2 cut(s) 217, 946
BmeRI GACNNNNNGTC 1 cut(s) 924
BmeT110I CYCGRG 1 cut(s) 908
BmgT120I GGNCC 2 cut(s) 217, 946
BmrFI CCNGG 2 cut(s) 160, 1407
BmsI GCATC 3 cut(s) 520, 841, 1103
BpiI GAAGAC 1 cut(s) 317
BplI GAGNNNNNCTC 2 cut(s) 484, 516
Bpu10I CCTNAGC 1 cut(s) 1401
BpuEI CTTGAG 3 cut(s) 386, 670, 998
BpuMI CCSGG 2 cut(s) 160, 1407
BsaI GGTCTC 1 cut(s) 247
BsaJI CCNNGG 1 cut(s) 220
BsaWI WCCGGW 1 cut(s) 985
BsaXI ACNNNNNCTCC 4 cut(s) 1023, 1053, 1245, 1275
Bsc4I CCNNNNNNNGG 3 cut(s) 159, 1203, 1406
Bse118I RCCGGY 1 cut(s) 1180
Bse1I ACTGG 1 cut(s) 1342
BseDI CCNNGG 1 cut(s) 220
BseGI GGATG 5 cut(s) 535, 660, 994, 1041, 1118
BseLI CCNNNNNNNGG 3 cut(s) 159, 1203, 1406
BseMII CTCAG 1 cut(s) 1392
BseNI ACTGG 1 cut(s) 1342
BseRI GAGGAG 1 cut(s) 983
BseSI GKGCMC 2 cut(s) 113, 1236
BseXI GCAGC 4 cut(s) 72, 267, 1311, 1499
BseYI CCCAGC 1 cut(s) 1139
Bsh1236I CGCG 1 cut(s) 209
Bsh1285I CGRYCG 1 cut(s) 986
BshFI GGCC 2 cut(s) 686, 1341
BsiEI CGRYCG 1 cut(s) 986
BsiHKAI GWGCWC 2 cut(s) 113, 1236
BsiHKCI CYCGRG 1 cut(s) 908
BsiSI CCGG 4 cut(s) 159, 986, 1181, 1407
BslI CCNNNNNNNGG 3 cut(s) 159, 1203, 1406
BsmAI GTCTC 2 cut(s) 219, 247
BsmI GAATGC 1 cut(s) 357
BsnI GGCC 2 cut(s) 686, 1341
Bso31I GGTCTC 1 cut(s) 247
BsoBI CYCGRG 1 cut(s) 908
Bsp1286I GDGCHC 4 cut(s) 113, 165, 1236, 1407
Bsp143I GATC 6 cut(s) 166, 422, 763, 772, 1291, 1429
BspACI CCGC 4 cut(s) 209, 239, 618, 932
BspANI GGCC 2 cut(s) 686, 1341
BspCNI CTCAG 1 cut(s) 1393
BspFNI CGCG 1 cut(s) 209
BspHI TCATGA 1 cut(s) 90
BspPI GGATC 2 cut(s) 430, 780
BspTNI GGTCTC 1 cut(s) 247
BsrFI RCCGGY 1 cut(s) 1180
BsrI ACTGG 1 cut(s) 1342
BssAI RCCGGY 1 cut(s) 1180
BssECI CCNNGG 1 cut(s) 220
BssMI GATC 6 cut(s) 166, 422, 763, 772, 1291, 1429
Bst4CI ACNGT 5 cut(s) 460, 691, 793, 1099, 1238
Bst6I CTCTTC 2 cut(s) 19, 1146
BstAPI GCANNNNNTGC 1 cut(s) 539
BstC8I GCNNGC 3 cut(s) 915, 1137, 1178
BstDEI CTNAG 1 cut(s) 1401
BstF5I GGATG 5 cut(s) 535, 660, 994, 1041, 1118
BstFNI CGCG 1 cut(s) 209
BstH2I RGCGCY 1 cut(s) 638
BstHHI GCGC 4 cut(s) 60, 209, 637, 1427
BstKTI GATC 6 cut(s) 169, 425, 766, 775, 1294, 1432
BstMAI GTCTC 2 cut(s) 219, 247
BstMBI GATC 6 cut(s) 166, 422, 763, 772, 1291, 1429
BstMCI CGRYCG 1 cut(s) 986
BstMWI GCNNNNNNNGC 7 cut(s) 55, 264, 539, 624, 851, 919, 1422
BstNSI RCATGY 4 cut(s) 714, 817, 1180, 1327
BstSCI CCNGG 2 cut(s) 158, 1405
BstSLI GKGCMC 2 cut(s) 113, 1236
BstUI CGCG 1 cut(s) 209
BstV1I GCAGC 4 cut(s) 72, 267, 1311, 1499
BstV2I GAAGAC 1 cut(s) 317
BstX2I RGATCY 2 cut(s) 772, 1429
BstXI CCANNNNNNTGG 1 cut(s) 515
BstYI RGATCY 2 cut(s) 772, 1429
BsuRI GGCC 2 cut(s) 686, 1341
BtsCI GGATG 5 cut(s) 535, 660, 994, 1041, 1118
BtsI GCAGTG 1 cut(s) 113
BtsIMutI CAGTG 4 cut(s) 113, 687, 1104, 1349
Cac8I GCNNGC 3 cut(s) 915, 1137, 1178
CciI TCATGA 1 cut(s) 90
CfoI GCGC 4 cut(s) 60, 209, 637, 1427
Cfr10I RCCGGY 1 cut(s) 1180
Cfr13I GGNCC 2 cut(s) 217, 946
CseI GACGC 1 cut(s) 928
Csp6I GTAC 3 cut(s) 453, 749, 1381
CspCI CAANNNNNGTGG 2 cut(s) 676, 711
CviQI GTAC 3 cut(s) 453, 749, 1381
DdeI CTNAG 1 cut(s) 1401
DpnI GATC 6 cut(s) 168, 424, 765, 774, 1293, 1431
DpnII GATC 6 cut(s) 166, 422, 763, 772, 1291, 1429
DrdI GACNNNNNNGTC 2 cut(s) 239, 1216
DriI GACNNNNNGTC 1 cut(s) 924
DseDI GACNNNNNNGTC 2 cut(s) 239, 1216
Eam1104I CTCTTC 2 cut(s) 19, 1146
Eam1105I GACNNNNNGTC 1 cut(s) 924
EarI CTCTTC 2 cut(s) 19, 1146
Eco24I GRGCYC 2 cut(s) 165, 1407
Eco31I GGTCTC 1 cut(s) 247
Eco47I GGWCC 2 cut(s) 217, 946
Eco47III AGCGCT 1 cut(s) 636
Eco57I CTGAAG 3 cut(s) 1272, 1482, 1494
Eco88I CYCGRG 1 cut(s) 908
EcoT22I ATGCAT 1 cut(s) 1118
EcoT38I GRGCYC 2 cut(s) 165, 1407
FauNDI CATATG 2 cut(s) 510, 1437
FbaI TGATCA 1 cut(s) 763
Fnu4HI GCNGC 4 cut(s) 61, 256, 1300, 1488
FokI GGATG 5 cut(s) 542, 667, 1001, 1048, 1125
FriOI GRGCYC 2 cut(s) 165, 1407
Fsp4HI GCNGC 4 cut(s) 61, 256, 1300, 1488
FspBI CTAG 3 cut(s) 567, 638, 1497
FspI TGCGCA 1 cut(s) 59
GlaI GCGC 4 cut(s) 59, 208, 636, 1426
GluI GCNGC 4 cut(s) 61, 256, 1300, 1488
GsaI CCCAGC 1 cut(s) 1143
HaeII RGCGCY 1 cut(s) 638
HaeIII GGCC 2 cut(s) 686, 1341
HapII CCGG 4 cut(s) 159, 986, 1181, 1407
HgaI GACGC 1 cut(s) 928
HhaI GCGC 4 cut(s) 60, 209, 637, 1427
Hin6I GCGC 4 cut(s) 58, 207, 635, 1425
HinP1I GCGC 4 cut(s) 58, 207, 635, 1425
HindIII AAGCTT 1 cut(s) 974
HinfI GANTC 5 cut(s) 476, 904, 1087, 1267, 1454
HpaII CCGG 4 cut(s) 159, 986, 1181, 1407
HphI GGTGA 2 cut(s) 1195, 1250
Hpy166II GTNNAC 2 cut(s) 111, 1234
Hpy188I TCNGA 6 cut(s) 217, 224, 427, 1086, 1291, 1474
Hpy188III TCNNGA 4 cut(s) 91, 649, 955, 1264
Hpy8I GTNNAC 2 cut(s) 111, 1234
Hpy99I CGWCG 1 cut(s) 922
HpyAV CCTTC 4 cut(s) 862, 1040, 1252, 1362
HpyCH4III ACNGT 5 cut(s) 460, 691, 793, 1099, 1238
HpyCH4IV ACGT 1 cut(s) 783
HpyCH4V TGCA 9 cut(s) 111, 355, 533, 627, 1116, 1234, 1331, 1378, 1487
HpyF10VI GCNNNNNNNGC 7 cut(s) 55, 264, 539, 624, 851, 919, 1422
HpyF3I CTNAG 1 cut(s) 1401
HpySE526I ACGT 1 cut(s) 783
HspAI GCGC 4 cut(s) 58, 207, 635, 1425
Ksp22I TGATCA 1 cut(s) 763
Kzo9I GATC 6 cut(s) 166, 422, 763, 772, 1291, 1429
LmnI GCTCC 3 cut(s) 9, 830, 1173
Lsp1109I GCAGC 4 cut(s) 72, 267, 1311, 1499
LweI GCATC 3 cut(s) 520, 841, 1103
MaeI CTAG 3 cut(s) 567, 638, 1497
MaeII ACGT 1 cut(s) 783
MaeIII GTNAC 4 cut(s) 697, 787, 994, 1492
MalI GATC 6 cut(s) 168, 424, 765, 774, 1293, 1431
MboI GATC 6 cut(s) 166, 422, 763, 772, 1291, 1429
MfeI CAATTG 1 cut(s) 416
MflI RGATCY 2 cut(s) 772, 1429
MhlI GDGCHC 4 cut(s) 113, 165, 1236, 1407
MluCI AATT 6 cut(s) 379, 416, 613, 799, 807, 1065
MlyI GAGTC 2 cut(s) 1276, 1448
MmeI TCCRAC 2 cut(s) 945, 1257
Mph1103I ATGCAT 1 cut(s) 1118
MseI TTAA 2 cut(s) 806, 858
MslI CAYNNNNRTG 4 cut(s) 116, 513, 927, 1181
MspI CCGG 4 cut(s) 159, 986, 1181, 1407
MspR9I CCNGG 2 cut(s) 160, 1407
MunI CAATTG 1 cut(s) 416
Mva1269I GAATGC 1 cut(s) 357
MvnI CGCG 1 cut(s) 209
MwoI GCNNNNNNNGC 7 cut(s) 55, 264, 539, 624, 851, 919, 1422
NciI CCSGG 2 cut(s) 160, 1407
NdeI CATATG 2 cut(s) 510, 1437
NdeII GATC 6 cut(s) 166, 422, 763, 772, 1291, 1429
NmeAIII GCCGAG 2 cut(s) 165, 686
NmuCI GTSAC 2 cut(s) 697, 787
NsbI TGCGCA 1 cut(s) 59
NsiI ATGCAT 1 cut(s) 1118
NspI RCATGY 4 cut(s) 714, 817, 1180, 1327
PaeI GCATGC 1 cut(s) 1180
PagI TCATGA 1 cut(s) 90
PciI ACATGT 2 cut(s) 813, 1323
PcsI WCGNNNNNNNCGW 1 cut(s) 1242
PctI GAATGC 1 cut(s) 357
PfeI GAWTC 3 cut(s) 476, 904, 1087
PflFI GACNNNGTC 1 cut(s) 793
PflMI CCANNNNNTGG 1 cut(s) 1203
PkrI GCNGC 4 cut(s) 62, 257, 1301, 1489
PleI GAGTC 2 cut(s) 1275, 1448
PpsI GAGTC 2 cut(s) 1275, 1448
PscI ACATGT 2 cut(s) 813, 1323
Psp1406I AACGTT 1 cut(s) 783
PspFI CCCAGC 1 cut(s) 1139
PspPI GGNCC 2 cut(s) 217, 946
PsuI RGATCY 2 cut(s) 772, 1429
PsyI GACNNNGTC 1 cut(s) 793
RsaI GTAC 3 cut(s) 454, 750, 1382
RsaNI GTAC 3 cut(s) 453, 749, 1381
RseI CAYNNNNRTG 4 cut(s) 116, 513, 927, 1181
SaqAI TTAA 2 cut(s) 806, 858
SatI GCNGC 4 cut(s) 61, 256, 1300, 1488
Sau3AI GATC 6 cut(s) 166, 422, 763, 772, 1291, 1429
Sau96I GGNCC 2 cut(s) 217, 946
SchI GAGTC 2 cut(s) 1276, 1448
ScrFI CCNGG 2 cut(s) 160, 1407
SduI GDGCHC 4 cut(s) 113, 165, 1236, 1407
SfaNI GCATC 3 cut(s) 520, 841, 1103
SinI GGWCC 2 cut(s) 217, 946
SmiMI CAYNNNNRTG 4 cut(s) 116, 513, 927, 1181
SmlI CTYRAG 3 cut(s) 401, 649, 977
SmoI CTYRAG 3 cut(s) 401, 649, 977
SpeI ACTAGT 1 cut(s) 1496
SphI GCATGC 1 cut(s) 1180
Sse9I AATT 6 cut(s) 379, 416, 613, 799, 807, 1065
SsiI CCGC 4 cut(s) 209, 239, 618, 932
SspMI CTAG 3 cut(s) 567, 638, 1497
StyD4I CCNGG 2 cut(s) 158, 1405
TaaI ACNGT 5 cut(s) 460, 691, 793, 1099, 1238
TaiI ACGT 1 cut(s) 786
TaqI TCGA 3 cut(s) 165, 555, 1245
TasI AATT 6 cut(s) 379, 416, 613, 799, 807, 1065
TatI WGTACW 2 cut(s) 452, 1380
TfiI GAWTC 3 cut(s) 476, 904, 1087
Tru1I TTAA 2 cut(s) 806, 858
Tru9I TTAA 2 cut(s) 806, 858
TscAI CASTG 4 cut(s) 113, 694, 1104, 1349
TseFI GTSAC 2 cut(s) 697, 787
TseI GCWGC 4 cut(s) 60, 255, 1299, 1487
Tsp45I GTSAC 2 cut(s) 697, 787
TspDTI ATGAA 7 cut(s) 134, 573, 881, 1059, 1424, 1454, 1491
TspGWI ACGGA 2 cut(s) 785, 1125
TspRI CASTG 4 cut(s) 113, 694, 1104, 1349
Tth111I GACNNNGTC 1 cut(s) 793
Van91I CCANNNNNTGG 1 cut(s) 1203
VneI GTGCAC 2 cut(s) 109, 1232
VpaK11BI GGWCC 2 cut(s) 217, 946
XceI RCATGY 4 cut(s) 714, 817, 1180, 1327
XspI CTAG 3 cut(s) 567, 638, 1497
Zsp2I ATGCAT 1 cut(s) 1118
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.