Rh7AG045600

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0017728
Physical Location & Seq
Reverse (-)
3080303 .. 3081574
1272 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG045600.1

Sequence Viewer

Length: 1272 bp
ATGGACTATGCGGTCTCTGTGTTCAGCAGCATTGAAAGCCCAGATGGGTTTTTATGGAACACCATGATTAGAGGGTTTGGGAAGACTGATAAGCCCGAAAGGGCGTTTGAGTTTTGTAAGAGAATGCAGGAGAAAGGAAATGTAGCTGACAATTTTACCCTCTCGTTCTTGCTCAAGGTTTCTGGGCAATTGGGATCGGATATATTGGGGAAGCAGATACATTGTACTACTGTGAAATATGGGTTTGATTCTAATGTGTTTGTGAGGAACACTCTAATCCATATGTATGGTATGTTTGGGGATGCAAAAACTGCTCTCCACCTGTTCGATGAAATGCCTAGCCCAGATTTAGTGGCGTGGAATACCATTATTGATAGTCATGTGAATTGCGGAAAGTGCAACGAAGCGCTAGATTTGTTCTTGAGGATGATGGATAGGGGTGTAGATACTGACGAGGCCACTGTCGTTGTGACCCTCTCGGCATGTTCTACATTGGGTGCTTTAGATTTTGGGAGGTGGGTACATTGTTTGGTTGATCATACGGATCTTGGCAACGTTGTGACGGTGTCTAATTCGTTAATTGACATGTATGCAAAATGTGGAGCTGTTGAAGAAGCGTATGATGCTTTTAATAAGATGAAGGGGAAGAAGAACATAGTATCTTGGAACACGATGATTCTCGGGCTTGCGACGCATGGTCATGCGGACAAATCTTTGGAACTGTTCTTGAAAATGTTGGAGGAGAAGCTTGAGAGACCGGATGGTGTTACTTTCTTGGGAGTTCTGTGTGCTTGTAGTCATGGAGGGATGGTTGATGAAGGAAGAAGATATTTTGAAATTATGAGCCAAGACTATCAGATTCAACCAACAGTGAAGCACTACGGATGCATGGTTGATATGTTGGGGCGAGCTGGGTTTGTGGAGGAGGCTTATAGGTTGATAAGGAGCATGCCGGTGAAGTGTAATGCCATTGTATGGAGGACATTGTTGTCGGCATGTCAGGTGCACGGTGAGGTCGAGCTTGGAGAGAAGGTCAGGAGTCATCTTTTGGAGTTGGCTTCAGATCATAGCAGCGATTATGTGCTTCTGGCTAACATGTATGCAAGTTTAGGCCAGTGGAACGAAGCTATGAGAGTAAGAAGGTCAATGCAGTACAGGGGAGTTGAGAAACCTGAGCCCGGTAATAGCTTGGTTGGCGCAGATCTTCATATGAAGTTGAAAATGGACTCAACTGAAGCCTATTCTGAAGATATAAATGCAGCAGTTTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

423

Amino Acids

47.31

Weight (kDa)

5.55

Isoelectric Point (pI)

39.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 14 - 59 4.1e-08 PPR repeat family
PPR PF01535 18 - 46 4.9e-07 PPR repeat
PPR_2 PF13041 115 - 163 4.7e-10 PPR repeat family
PPR PF01535 118 - 148 7.1e-07 PPR repeat
PPR_2 PF13041 217 - 266 8.3e-07 PPR repeat family
E_motif PF20431 336 - 398 1.5e-14 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017728)

Species Orthologous Gene IDs
malus_domestica MD06G1195900.v1.1
prunus_persica Prupe.5G195700_v2.0.a1
pyrus_communis pycom06g17310
rosa_chinensis RchiOBHm_Chr7g0181731
rosa_laevigata RLG00000005173
rosa_multiflora Rmu_sc0000988.1_g000008
rosa_roxburghii Rroxscaffold_3G00271770
rosa_rugosa Rorug06G0443000
rosa_samantha Rh7AG045600 Rh7BG044800 Rh7CG046300
rosa_wichuraiana Rw7G003800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 11, 988
AccB7I CCANNNNNTGG 1 cut(s) 975
AciI CCGC 3 cut(s) 11, 390, 704
AclI AACGTT 1 cut(s) 555
AclWI GGATC 2 cut(s) 202, 552
AcuI CTGAAG 3 cut(s) 1044, 1254, 1266
AfaI GTAC 3 cut(s) 226, 522, 1154
AfeI AGCGCT 1 cut(s) 408
AfiI CCNNNNNNNGG 2 cut(s) 975, 1178
AflIII ACRYGT 2 cut(s) 585, 1095
AgsI TTSAA 6 cut(s) 35, 611, 730, 836, 863, 1219
AhdI GACNNNNNGTC 1 cut(s) 696
AluBI AGCT 7 cut(s) 146, 605, 748, 911, 1021, 1127, 1188
AluI AGCT 7 cut(s) 146, 605, 748, 911, 1021, 1127, 1188
Alw21I GWGCWC 1 cut(s) 1008
Alw26I GTCTC 2 cut(s) 19, 748
Alw44I GTGCAC 1 cut(s) 1004
AlwI GGATC 2 cut(s) 202, 552
Ama87I CYCGRG 1 cut(s) 680
Aor51HI AGCGCT 1 cut(s) 408
AoxI GGCC 2 cut(s) 456, 1111
ApaLI GTGCAC 1 cut(s) 1004
ApeKI GCWGC 3 cut(s) 27, 1071, 1259
AspLEI GCGC 2 cut(s) 409, 1199
AsuC2I CCSGG 1 cut(s) 1179
AsuHPI GGTGA 2 cut(s) 967, 1022
AvaI CYCGRG 1 cut(s) 680
BaeGI GKGCMC 1 cut(s) 1008
BanII GRGCYC 1 cut(s) 1179
BbsI GAAGAC 1 cut(s) 89
Bbv12I GWGCWC 1 cut(s) 1008
BbvI GCAGC 2 cut(s) 39, 1083
BccI CCATC 4 cut(s) 38, 424, 755, 802
BclI TGATCA 1 cut(s) 535
BcnI CCSGG 1 cut(s) 1179
BcoDI GTCTC 2 cut(s) 19, 748
BfaI CTAG 3 cut(s) 339, 410, 1270
BfoI RGCGCY 1 cut(s) 410
BglII AGATCT 1 cut(s) 1201
BisI GCNGC 3 cut(s) 28, 1072, 1260
BlsI GCNGC 3 cut(s) 29, 1073, 1261
Bme1390I CCNGG 1 cut(s) 1179
BmeRI GACNNNNNGTC 1 cut(s) 696
BmeT110I CYCGRG 1 cut(s) 680
BmrFI CCNGG 1 cut(s) 1179
BmsI GCATC 3 cut(s) 292, 613, 875
BpiI GAAGAC 1 cut(s) 89
BplI GAGNNNNNCTC 2 cut(s) 256, 288
Bpu10I CCTNAGC 1 cut(s) 1173
BpuEI CTTGAG 3 cut(s) 158, 442, 770
BpuMI CCSGG 1 cut(s) 1179
BsaI GGTCTC 2 cut(s) 19, 748
BsaWI WCCGGW 1 cut(s) 757
BsaXI ACNNNNNCTCC 4 cut(s) 795, 825, 1017, 1047
Bsc4I CCNNNNNNNGG 2 cut(s) 975, 1178
Bse118I RCCGGY 1 cut(s) 952
Bse1I ACTGG 1 cut(s) 1114
BseGI GGATG 5 cut(s) 307, 432, 766, 813, 890
BseLI CCNNNNNNNGG 2 cut(s) 975, 1178
BseMII CTCAG 1 cut(s) 1164
BseNI ACTGG 1 cut(s) 1114
BseRI GAGGAG 2 cut(s) 755, 938
BseSI GKGCMC 1 cut(s) 1008
BseXI GCAGC 2 cut(s) 39, 1083
BseYI CCCAGC 1 cut(s) 911
BshFI GGCC 2 cut(s) 458, 1113
BsiHKAI GWGCWC 1 cut(s) 1008
BsiHKCI CYCGRG 1 cut(s) 680
BsiSI CCGG 3 cut(s) 758, 953, 1179
BslI CCNNNNNNNGG 2 cut(s) 975, 1178
BsmAI GTCTC 2 cut(s) 19, 748
BsmI GAATGC 1 cut(s) 129
BsnI GGCC 2 cut(s) 458, 1113
Bso31I GGTCTC 2 cut(s) 19, 748
BsoBI CYCGRG 1 cut(s) 680
Bsp1286I GDGCHC 2 cut(s) 1008, 1179
Bsp143I GATC 5 cut(s) 194, 535, 544, 1063, 1201
BspACI CCGC 3 cut(s) 11, 390, 704
BspANI GGCC 2 cut(s) 458, 1113
BspCNI CTCAG 1 cut(s) 1165
BspPI GGATC 2 cut(s) 202, 552
BspTNI GGTCTC 2 cut(s) 19, 748
BsrFI RCCGGY 1 cut(s) 952
BsrI ACTGG 1 cut(s) 1114
BssAI RCCGGY 1 cut(s) 952
BssMI GATC 5 cut(s) 194, 535, 544, 1063, 1201
Bst4CI ACNGT 6 cut(s) 232, 463, 565, 723, 871, 1010
BstAPI GCANNNNNTGC 1 cut(s) 311
BstC8I GCNNGC 3 cut(s) 687, 909, 950
BstDEI CTNAG 1 cut(s) 1173
BstF5I GGATG 5 cut(s) 307, 432, 766, 813, 890
BstH2I RGCGCY 1 cut(s) 410
BstHHI GCGC 2 cut(s) 409, 1199
BstKTI GATC 5 cut(s) 197, 538, 547, 1066, 1204
BstMAI GTCTC 2 cut(s) 19, 748
BstMBI GATC 5 cut(s) 194, 535, 544, 1063, 1201
BstMWI GCNNNNNNNGC 6 cut(s) 36, 311, 396, 623, 691, 1194
BstNSI RCATGY 5 cut(s) 486, 589, 952, 999, 1099
BstSCI CCNGG 1 cut(s) 1177
BstSLI GKGCMC 1 cut(s) 1008
BstV1I GCAGC 2 cut(s) 39, 1083
BstV2I GAAGAC 1 cut(s) 89
BstX2I RGATCY 2 cut(s) 544, 1201
BstXI CCANNNNNNTGG 1 cut(s) 287
BstYI RGATCY 2 cut(s) 544, 1201
BsuRI GGCC 2 cut(s) 458, 1113
BtsCI GGATG 5 cut(s) 307, 432, 766, 813, 890
BtsIMutI CAGTG 3 cut(s) 459, 876, 1121
Cac8I GCNNGC 3 cut(s) 687, 909, 950
CfoI GCGC 2 cut(s) 409, 1199
Cfr10I RCCGGY 1 cut(s) 952
CseI GACGC 1 cut(s) 700
Csp6I GTAC 3 cut(s) 225, 521, 1153
CspCI CAANNNNNGTGG 2 cut(s) 448, 483
CviQI GTAC 3 cut(s) 225, 521, 1153
DdeI CTNAG 1 cut(s) 1173
DpnI GATC 5 cut(s) 196, 537, 546, 1065, 1203
DpnII GATC 5 cut(s) 194, 535, 544, 1063, 1201
DrdI GACNNNNNNGTC 2 cut(s) 11, 988
DriI GACNNNNNGTC 1 cut(s) 696
DseDI GACNNNNNNGTC 2 cut(s) 11, 988
Eam1105I GACNNNNNGTC 1 cut(s) 696
Eco24I GRGCYC 1 cut(s) 1179
Eco31I GGTCTC 2 cut(s) 19, 748
Eco47III AGCGCT 1 cut(s) 408
Eco57I CTGAAG 3 cut(s) 1044, 1254, 1266
Eco88I CYCGRG 1 cut(s) 680
EcoT22I ATGCAT 1 cut(s) 890
EcoT38I GRGCYC 1 cut(s) 1179
FauNDI CATATG 2 cut(s) 282, 1209
FbaI TGATCA 1 cut(s) 535
Fnu4HI GCNGC 3 cut(s) 28, 1072, 1260
FokI GGATG 5 cut(s) 314, 439, 773, 820, 897
FriOI GRGCYC 1 cut(s) 1179
Fsp4HI GCNGC 3 cut(s) 28, 1072, 1260
FspBI CTAG 3 cut(s) 339, 410, 1270
GlaI GCGC 2 cut(s) 408, 1198
GluI GCNGC 3 cut(s) 28, 1072, 1260
GsaI CCCAGC 1 cut(s) 915
HaeII RGCGCY 1 cut(s) 410
HaeIII GGCC 2 cut(s) 458, 1113
HapII CCGG 3 cut(s) 758, 953, 1179
HgaI GACGC 1 cut(s) 700
HhaI GCGC 2 cut(s) 409, 1199
Hin6I GCGC 2 cut(s) 407, 1197
HinP1I GCGC 2 cut(s) 407, 1197
HindIII AAGCTT 1 cut(s) 746
HinfI GANTC 5 cut(s) 248, 676, 859, 1039, 1226
HpaII CCGG 3 cut(s) 758, 953, 1179
HphI GGTGA 2 cut(s) 967, 1022
Hpy166II GTNNAC 2 cut(s) 1006, 1267
Hpy188I TCNGA 4 cut(s) 199, 858, 1063, 1246
Hpy188III TCNNGA 3 cut(s) 421, 727, 1036
Hpy8I GTNNAC 2 cut(s) 1006, 1267
Hpy99I CGWCG 1 cut(s) 694
HpyAV CCTTC 4 cut(s) 634, 812, 1024, 1134
HpyCH4III ACNGT 6 cut(s) 232, 463, 565, 723, 871, 1010
HpyCH4IV ACGT 1 cut(s) 555
HpyCH4V TGCA 9 cut(s) 127, 305, 399, 593, 888, 1006, 1103, 1150, 1259
HpyF10VI GCNNNNNNNGC 6 cut(s) 36, 311, 396, 623, 691, 1194
HpyF3I CTNAG 1 cut(s) 1173
HpySE526I ACGT 1 cut(s) 555
HspAI GCGC 2 cut(s) 407, 1197
Ksp22I TGATCA 1 cut(s) 535
Kzo9I GATC 5 cut(s) 194, 535, 544, 1063, 1201
LmnI GCTCC 2 cut(s) 602, 945
Lsp1109I GCAGC 2 cut(s) 39, 1083
LweI GCATC 3 cut(s) 292, 613, 875
MaeI CTAG 3 cut(s) 339, 410, 1270
MaeII ACGT 1 cut(s) 555
MaeIII GTNAC 3 cut(s) 469, 559, 766
MalI GATC 5 cut(s) 196, 537, 546, 1065, 1203
MboI GATC 5 cut(s) 194, 535, 544, 1063, 1201
MboII GAAGA 8 cut(s) 94, 623, 658, 661, 834, 837, 1196, 1259
MfeI CAATTG 1 cut(s) 188
MflI RGATCY 2 cut(s) 544, 1201
MhlI GDGCHC 2 cut(s) 1008, 1179
MluCI AATT 6 cut(s) 151, 188, 385, 571, 579, 837
MlyI GAGTC 2 cut(s) 1048, 1220
MmeI TCCRAC 1 cut(s) 717
Mph1103I ATGCAT 1 cut(s) 890
MseI TTAA 2 cut(s) 578, 630
MslI CAYNNNNRTG 3 cut(s) 285, 699, 953
MspI CCGG 3 cut(s) 758, 953, 1179
MspR9I CCNGG 1 cut(s) 1179
MunI CAATTG 1 cut(s) 188
Mva1269I GAATGC 1 cut(s) 129
MwoI GCNNNNNNNGC 6 cut(s) 36, 311, 396, 623, 691, 1194
NciI CCSGG 1 cut(s) 1179
NdeI CATATG 2 cut(s) 282, 1209
NdeII GATC 5 cut(s) 194, 535, 544, 1063, 1201
NmeAIII GCCGAG 1 cut(s) 458
NmuCI GTSAC 2 cut(s) 469, 559
NsiI ATGCAT 1 cut(s) 890
NspI RCATGY 5 cut(s) 486, 589, 952, 999, 1099
PaeI GCATGC 1 cut(s) 952
PciI ACATGT 2 cut(s) 585, 1095
PcsI WCGNNNNNNNCGW 1 cut(s) 1014
PctI GAATGC 1 cut(s) 129
PfeI GAWTC 3 cut(s) 248, 676, 859
PflFI GACNNNGTC 1 cut(s) 565
PflMI CCANNNNNTGG 1 cut(s) 975
PkrI GCNGC 3 cut(s) 29, 1073, 1261
PleI GAGTC 2 cut(s) 1047, 1220
PpsI GAGTC 2 cut(s) 1047, 1220
PscI ACATGT 2 cut(s) 585, 1095
Psp1406I AACGTT 1 cut(s) 555
PspFI CCCAGC 1 cut(s) 911
PsuI RGATCY 2 cut(s) 544, 1201
PsyI GACNNNGTC 1 cut(s) 565
RsaI GTAC 3 cut(s) 226, 522, 1154
RsaNI GTAC 3 cut(s) 225, 521, 1153
RseI CAYNNNNRTG 3 cut(s) 285, 699, 953
SaqAI TTAA 2 cut(s) 578, 630
SatI GCNGC 3 cut(s) 28, 1072, 1260
Sau3AI GATC 5 cut(s) 194, 535, 544, 1063, 1201
SchI GAGTC 2 cut(s) 1048, 1220
ScrFI CCNGG 1 cut(s) 1179
SduI GDGCHC 2 cut(s) 1008, 1179
SfaNI GCATC 3 cut(s) 292, 613, 875
SmiMI CAYNNNNRTG 3 cut(s) 285, 699, 953
SmlI CTYRAG 3 cut(s) 173, 421, 749
SmoI CTYRAG 3 cut(s) 173, 421, 749
SphI GCATGC 1 cut(s) 952
Sse9I AATT 6 cut(s) 151, 188, 385, 571, 579, 837
SsiI CCGC 3 cut(s) 11, 390, 704
SspMI CTAG 3 cut(s) 339, 410, 1270
StyD4I CCNGG 1 cut(s) 1177
TaaI ACNGT 6 cut(s) 232, 463, 565, 723, 871, 1010
TaiI ACGT 1 cut(s) 558
TaqI TCGA 2 cut(s) 327, 1017
TasI AATT 6 cut(s) 151, 188, 385, 571, 579, 837
TatI WGTACW 2 cut(s) 224, 1152
TfiI GAWTC 3 cut(s) 248, 676, 859
Tru1I TTAA 2 cut(s) 578, 630
Tru9I TTAA 2 cut(s) 578, 630
TscAI CASTG 3 cut(s) 466, 876, 1121
TseFI GTSAC 2 cut(s) 469, 559
TseI GCWGC 3 cut(s) 27, 1071, 1259
Tsp45I GTSAC 2 cut(s) 469, 559
TspDTI ATGAA 5 cut(s) 345, 653, 831, 1196, 1226
TspGWI ACGGA 2 cut(s) 557, 897
TspRI CASTG 3 cut(s) 466, 876, 1121
Tth111I GACNNNGTC 1 cut(s) 565
Van91I CCANNNNNTGG 1 cut(s) 975
VneI GTGCAC 1 cut(s) 1004
XceI RCATGY 5 cut(s) 486, 589, 952, 999, 1099
XspI CTAG 3 cut(s) 339, 410, 1270
Zsp2I ATGCAT 1 cut(s) 890
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.