RLG00000005173

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0017728
Physical Location & Seq
Forward (+)
65642254 .. 65643699
1446 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005173

Sequence Viewer

Length: 1446 bp
ATGCGAAGCAACATATTTCATAAAGCTCAAAGGGTCATGACCCTTTTCAAGCAGTGCACCACCATGAAAGAGGTCAAGCAACTCCATGCCCATATAACCCACACCGGGCTCGATCAAACCCTCTGTGTTCTCGGCAAGGTCATCTCATTTTGCGCGGTTTCGGACCTCGGTGACATGGACTATGCGTTCTCTGTGTTCAGCAGCATTGAAAGCCCAGATGGGTTTTTATGGAACACCATGATTAGAGGGTTTGGGAAGACTGATAAACCCGAAAGGGCGTTTGAGTTTTGTAAGAGAATGCAGGAGAAAGGGAATGTAGCTGACAATTTTACCCTCTCGTTCTTGCTCAAGGTTTCTGGGCAATTGGGATCGGATATATTGGGGAAGCAGATACATTGTACTGCTGTGAAATATGGGTTTGATTCTAATGTGTTTGTGAGGAACACTCTAATCCATATGTATGGTATGTTTGGGGATGCAAAAACTGCTCTCCACCTGTTCGATGAAATGCCTAGCCCAGATTTAGTGGCGTGGAATACCATTATTGATAGTCATGTCAATTGCGGAAAGTGCAACGAAGCGCTATATTTGTTCTTGAGGATGATGGATAGGGGTGTAGAGCCTGACGAGGCCACTGTGGTTGTGACCCTCTCGGCATGTTCTACATTGGGTGCTTTAGATTTTGGGAGGTGGGTGCATTGTTTGGTTGATCATTTGGATCTTGGCAACGTTGTGACGGTGTCTAATTCGTTAATTGACATGTATGCGAAATGTGGAGCTGTTGAAGAAGCGTATGATGCTTTTAATAAGATGAAGGGCAAGAAGAACATAGTATCTTGGAACACGATGATTCTCGGGCTTGCGACGCATGGTCATGCGGACAAATCTTTGGAACTGTTCTTGAAAATGTTGGAGGAGAAGCTTGAGAGACCGGATGGTGTTACTTTCTTGGGAGTTCTGTGTGCTTGTAGTCATGGAGGGATGGTTGATGAAGGAAGAAGATATTTTGAAATTATGAGCAAAGACTATCAGATTCAACCAACACTGAAGCACTACGGATGCATGGTTGATATGTTGGGGCGAGCTGGGTTTGTGGAGGAGGCTTATAGGTTGATAAGGAGCATGCCGGTTAAGTGTAATGCCATTGTATGGAGGACATTGTTGTCGGCGTGTCAGGTGCACGGTGAGGCCGAGCTTGGAGAGAAGGTCAGGAATCATCTTTTGGAGTTGGATTCAGATCATAGCAGCGATTATGTGCTTCTGGCTAACACGTATGCAAGTTTAGGCCAGTGGAATGAAGCTATGAGAGTAAGAAGATCAATGCAGTACAGGGGAGTTGAGAAACCTGAGCCTGGTAATAGCTTGGTTGGTGCAGATCTTCATATGAAGTTGAAAATGGACTCAACTGAAGCCTATTCTGAGGATATAAATGCAGCAGTTTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

482

Amino Acids

53.94

Weight (kDa)

6.06

Isoelectric Point (pI)

38.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 72 - 117 4.9e-08 PPR repeat family
PPR PF01535 76 - 104 5.8e-07 PPR repeat
PPR_2 PF13041 173 - 221 4.6e-11 PPR repeat family
PPR PF01535 176 - 206 5.9e-06 PPR repeat
PPR_2 PF13041 275 - 324 9.9e-07 PPR repeat family
E_motif PF20431 394 - 456 5.4e-15 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017728)

Species Orthologous Gene IDs
malus_domestica MD06G1195900.v1.1
prunus_persica Prupe.5G195700_v2.0.a1
pyrus_communis pycom06g17310
rosa_chinensis RchiOBHm_Chr7g0181731
rosa_laevigata RLG00000005173
rosa_multiflora Rmu_sc0000988.1_g000008
rosa_roxburghii Rroxscaffold_3G00271770
rosa_rugosa Rorug06G0443000
rosa_samantha Rh7AG045600 Rh7BG044800 Rh7CG046300
rosa_wichuraiana Rw7G003800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1162
AccB7I CCANNNNNTGG 1 cut(s) 1149
AccII CGCG 1 cut(s) 155
AciI CCGC 3 cut(s) 155, 564, 878
AclI AACGTT 1 cut(s) 729
AclWI GGATC 2 cut(s) 376, 726
AcuI CTGAAG 2 cut(s) 1067, 1428
AfaI GTAC 2 cut(s) 400, 1328
AfeI AGCGCT 1 cut(s) 582
AfiI CCNNNNNNNGG 3 cut(s) 105, 1149, 1352
AflIII ACRYGT 2 cut(s) 759, 1269
AgsI TTSAA 7 cut(s) 49, 209, 785, 904, 1010, 1037, 1393
AhdI GACNNNNNGTC 1 cut(s) 870
AjnI CCWGG 1 cut(s) 1351
AjuI GAANNNNNNNTTGG 2 cut(s) 1205, 1237
AloI GAACNNNNNNTCC 2 cut(s) 170, 202
AluBI AGCT 8 cut(s) 26, 320, 779, 922, 1085, 1195, 1301, 1362
AluI AGCT 8 cut(s) 26, 320, 779, 922, 1085, 1195, 1301, 1362
Alw21I GWGCWC 2 cut(s) 59, 1182
Alw26I GTCTC 1 cut(s) 922
Alw44I GTGCAC 2 cut(s) 55, 1178
AlwI GGATC 2 cut(s) 376, 726
Ama87I CYCGRG 1 cut(s) 854
Aor51HI AGCGCT 1 cut(s) 582
AoxI GGCC 3 cut(s) 630, 1188, 1285
ApaLI GTGCAC 2 cut(s) 55, 1178
ApeKI GCWGC 3 cut(s) 201, 1245, 1433
AspLEI GCGC 2 cut(s) 155, 583
AspS9I GGNCC 1 cut(s) 163
AsuC2I CCSGG 1 cut(s) 106
AsuHPI GGTGA 2 cut(s) 182, 1196
AvaI CYCGRG 1 cut(s) 854
AvaII GGWCC 1 cut(s) 163
BaeGI GKGCMC 2 cut(s) 59, 1182
BanII GRGCYC 1 cut(s) 111
BbsI GAAGAC 1 cut(s) 263
Bbv12I GWGCWC 2 cut(s) 59, 1182
BbvI GCAGC 2 cut(s) 213, 1257
BccI CCATC 4 cut(s) 212, 598, 929, 976
BciT130I CCWGG 1 cut(s) 1353
BclI TGATCA 1 cut(s) 709
BcnI CCSGG 1 cut(s) 106
BcoDI GTCTC 1 cut(s) 922
BfaI CTAG 2 cut(s) 513, 1444
BfoI RGCGCY 1 cut(s) 584
BglII AGATCT 1 cut(s) 1375
BisI GCNGC 3 cut(s) 202, 1246, 1434
BlsI GCNGC 3 cut(s) 203, 1247, 1435
Bme1390I CCNGG 2 cut(s) 106, 1353
Bme18I GGWCC 1 cut(s) 163
BmeRI GACNNNNNGTC 1 cut(s) 870
BmeT110I CYCGRG 1 cut(s) 854
BmgT120I GGNCC 1 cut(s) 163
BmrFI CCNGG 2 cut(s) 106, 1353
BmsI GCATC 3 cut(s) 466, 787, 1049
BpiI GAAGAC 1 cut(s) 263
BplI GAGNNNNNCTC 2 cut(s) 430, 462
Bpu10I CCTNAGC 1 cut(s) 1347
BpuEI CTTGAG 3 cut(s) 332, 616, 944
BpuMI CCSGG 1 cut(s) 106
BsaAI YACGTR 1 cut(s) 1272
BsaBI GATNNNNATC 1 cut(s) 1236
BsaI GGTCTC 1 cut(s) 922
BsaJI CCNNGG 1 cut(s) 166
BsaWI WCCGGW 1 cut(s) 931
BsaXI ACNNNNNCTCC 4 cut(s) 969, 999, 1191, 1221
Bsc4I CCNNNNNNNGG 3 cut(s) 105, 1149, 1352
Bse118I RCCGGY 1 cut(s) 1126
Bse1I ACTGG 1 cut(s) 1288
Bse8I GATNNNNATC 1 cut(s) 1236
BseBI CCWGG 1 cut(s) 1353
BseDI CCNNGG 1 cut(s) 166
BseGI GGATG 5 cut(s) 481, 606, 940, 987, 1064
BseJI GATNNNNATC 1 cut(s) 1236
BseLI CCNNNNNNNGG 3 cut(s) 105, 1149, 1352
BseMII CTCAG 2 cut(s) 1338, 1410
BseNI ACTGG 1 cut(s) 1288
BseRI GAGGAG 2 cut(s) 929, 1112
BseSI GKGCMC 2 cut(s) 59, 1182
BseXI GCAGC 2 cut(s) 213, 1257
BseYI CCCAGC 1 cut(s) 1085
BsgI GTGCAG 1 cut(s) 1392
Bsh1236I CGCG 1 cut(s) 155
BshFI GGCC 3 cut(s) 632, 1190, 1287
BsiHKAI GWGCWC 2 cut(s) 59, 1182
BsiHKCI CYCGRG 1 cut(s) 854
BsiSI CCGG 3 cut(s) 105, 932, 1127
BslI CCNNNNNNNGG 3 cut(s) 105, 1149, 1352
BsmAI GTCTC 1 cut(s) 922
BsmI GAATGC 1 cut(s) 303
BsnI GGCC 3 cut(s) 632, 1190, 1287
Bso31I GGTCTC 1 cut(s) 922
BsoBI CYCGRG 1 cut(s) 854
Bsp1286I GDGCHC 3 cut(s) 59, 111, 1182
Bsp143I GATC 7 cut(s) 112, 368, 709, 718, 1237, 1316, 1375
BspACI CCGC 3 cut(s) 155, 564, 878
BspANI GGCC 3 cut(s) 632, 1190, 1287
BspCNI CTCAG 2 cut(s) 1339, 1411
BspFNI CGCG 1 cut(s) 155
BspHI TCATGA 1 cut(s) 36
BspPI GGATC 2 cut(s) 376, 726
BspTNI GGTCTC 1 cut(s) 922
BsrFI RCCGGY 1 cut(s) 1126
BsrI ACTGG 1 cut(s) 1288
BssAI RCCGGY 1 cut(s) 1126
BssECI CCNNGG 1 cut(s) 166
BssMI GATC 7 cut(s) 112, 368, 709, 718, 1237, 1316, 1375
Bst2UI CCWGG 1 cut(s) 1353
Bst4CI ACNGT 4 cut(s) 637, 739, 897, 1184
BstAPI GCANNNNNTGC 1 cut(s) 485
BstBAI YACGTR 1 cut(s) 1272
BstC8I GCNNGC 3 cut(s) 861, 1083, 1124
BstDEI CTNAG 2 cut(s) 1347, 1419
BstF5I GGATG 5 cut(s) 481, 606, 940, 987, 1064
BstFNI CGCG 1 cut(s) 155
BstH2I RGCGCY 1 cut(s) 584
BstHHI GCGC 2 cut(s) 155, 583
BstKTI GATC 7 cut(s) 115, 371, 712, 721, 1240, 1319, 1378
BstMAI GTCTC 1 cut(s) 922
BstMBI GATC 7 cut(s) 112, 368, 709, 718, 1237, 1316, 1375
BstMWI GCNNNNNNNGC 5 cut(s) 210, 485, 570, 797, 865
BstNI CCWGG 1 cut(s) 1353
BstNSI RCATGY 3 cut(s) 660, 763, 1126
BstSCI CCNGG 2 cut(s) 104, 1351
BstSLI GKGCMC 2 cut(s) 59, 1182
BstUI CGCG 1 cut(s) 155
BstV1I GCAGC 2 cut(s) 213, 1257
BstV2I GAAGAC 1 cut(s) 263
BstX2I RGATCY 2 cut(s) 718, 1375
BstXI CCANNNNNNTGG 1 cut(s) 461
BstYI RGATCY 2 cut(s) 718, 1375
BsuRI GGCC 3 cut(s) 632, 1190, 1287
BtsCI GGATG 5 cut(s) 481, 606, 940, 987, 1064
BtsI GCAGTG 1 cut(s) 59
BtsIMutI CAGTG 4 cut(s) 59, 633, 1043, 1295
Cac8I GCNNGC 3 cut(s) 861, 1083, 1124
CciI TCATGA 1 cut(s) 36
CfoI GCGC 2 cut(s) 155, 583
Cfr10I RCCGGY 1 cut(s) 1126
Cfr13I GGNCC 1 cut(s) 163
CseI GACGC 1 cut(s) 874
Csp6I GTAC 2 cut(s) 399, 1327
CspCI CAANNNNNGTGG 2 cut(s) 622, 657
CviQI GTAC 2 cut(s) 399, 1327
DdeI CTNAG 2 cut(s) 1347, 1419
DpnI GATC 7 cut(s) 114, 370, 711, 720, 1239, 1318, 1377
DpnII GATC 7 cut(s) 112, 368, 709, 718, 1237, 1316, 1375
DrdI GACNNNNNNGTC 1 cut(s) 1162
DriI GACNNNNNGTC 1 cut(s) 870
DseDI GACNNNNNNGTC 1 cut(s) 1162
Eam1105I GACNNNNNGTC 1 cut(s) 870
Eco24I GRGCYC 1 cut(s) 111
Eco31I GGTCTC 1 cut(s) 922
Eco47I GGWCC 1 cut(s) 163
Eco47III AGCGCT 1 cut(s) 582
Eco57I CTGAAG 2 cut(s) 1067, 1428
Eco88I CYCGRG 1 cut(s) 854
EcoRII CCWGG 1 cut(s) 1351
EcoT22I ATGCAT 1 cut(s) 1064
EcoT38I GRGCYC 1 cut(s) 111
FauNDI CATATG 2 cut(s) 456, 1383
FbaI TGATCA 1 cut(s) 709
Fnu4HI GCNGC 3 cut(s) 202, 1246, 1434
FokI GGATG 5 cut(s) 488, 613, 947, 994, 1071
FriOI GRGCYC 1 cut(s) 111
Fsp4HI GCNGC 3 cut(s) 202, 1246, 1434
FspBI CTAG 2 cut(s) 513, 1444
GlaI GCGC 2 cut(s) 154, 582
GluI GCNGC 3 cut(s) 202, 1246, 1434
GsaI CCCAGC 1 cut(s) 1089
HaeII RGCGCY 1 cut(s) 584
HaeIII GGCC 3 cut(s) 632, 1190, 1287
HapII CCGG 3 cut(s) 105, 932, 1127
HgaI GACGC 1 cut(s) 874
HhaI GCGC 2 cut(s) 155, 583
Hin6I GCGC 2 cut(s) 153, 581
HinP1I GCGC 2 cut(s) 153, 581
HindIII AAGCTT 1 cut(s) 920
HinfI GANTC 6 cut(s) 422, 850, 1033, 1213, 1232, 1400
HpaII CCGG 3 cut(s) 105, 932, 1127
HphI GGTGA 2 cut(s) 182, 1196
Hpy166II GTNNAC 3 cut(s) 57, 1180, 1441
Hpy188I TCNGA 5 cut(s) 163, 373, 1032, 1237, 1420
Hpy188III TCNNGA 4 cut(s) 37, 595, 901, 1210
Hpy8I GTNNAC 3 cut(s) 57, 1180, 1441
Hpy99I CGWCG 1 cut(s) 868
HpyAV CCTTC 3 cut(s) 808, 986, 1198
HpyCH4III ACNGT 4 cut(s) 637, 739, 897, 1184
HpyCH4IV ACGT 2 cut(s) 729, 1271
HpyF10VI GCNNNNNNNGC 5 cut(s) 210, 485, 570, 797, 865
HpyF3I CTNAG 2 cut(s) 1347, 1419
HpySE526I ACGT 2 cut(s) 729, 1271
HspAI GCGC 2 cut(s) 153, 581
Ksp22I TGATCA 1 cut(s) 709
Kzo9I GATC 7 cut(s) 112, 368, 709, 718, 1237, 1316, 1375
LmnI GCTCC 2 cut(s) 776, 1119
Lsp1109I GCAGC 2 cut(s) 213, 1257
LweI GCATC 3 cut(s) 466, 787, 1049
MaeI CTAG 2 cut(s) 513, 1444
MaeII ACGT 2 cut(s) 729, 1271
MaeIII GTNAC 4 cut(s) 170, 643, 733, 940
MalI GATC 7 cut(s) 114, 370, 711, 720, 1239, 1318, 1377
MboI GATC 7 cut(s) 112, 368, 709, 718, 1237, 1316, 1375
MboII GAAGA 7 cut(s) 268, 797, 835, 1008, 1011, 1326, 1370
MfeI CAATTG 2 cut(s) 362, 559
MflI RGATCY 2 cut(s) 718, 1375
MhlI GDGCHC 3 cut(s) 59, 111, 1182
MluCI AATT 6 cut(s) 325, 362, 559, 745, 753, 1011
MlyI GAGTC 1 cut(s) 1394
MmeI TCCRAC 2 cut(s) 891, 1209
Mph1103I ATGCAT 1 cut(s) 1064
MseI TTAA 3 cut(s) 752, 804, 1131
MslI CAYNNNNRTG 3 cut(s) 62, 459, 873
MspI CCGG 3 cut(s) 105, 932, 1127
MspR9I CCNGG 2 cut(s) 106, 1353
MunI CAATTG 2 cut(s) 362, 559
Mva1269I GAATGC 1 cut(s) 303
MvaI CCWGG 1 cut(s) 1353
MvnI CGCG 1 cut(s) 155
MwoI GCNNNNNNNGC 5 cut(s) 210, 485, 570, 797, 865
NciI CCSGG 1 cut(s) 106
NdeI CATATG 2 cut(s) 456, 1383
NdeII GATC 7 cut(s) 112, 368, 709, 718, 1237, 1316, 1375
NmeAIII GCCGAG 3 cut(s) 111, 632, 1216
NmuCI GTSAC 3 cut(s) 170, 643, 733
NsiI ATGCAT 1 cut(s) 1064
NspI RCATGY 3 cut(s) 660, 763, 1126
PaeI GCATGC 1 cut(s) 1126
PagI TCATGA 1 cut(s) 36
PciI ACATGT 1 cut(s) 759
PcsI WCGNNNNNNNCGW 1 cut(s) 1188
PctI GAATGC 1 cut(s) 303
PfeI GAWTC 5 cut(s) 422, 850, 1033, 1213, 1232
PflFI GACNNNGTC 1 cut(s) 739
PflMI CCANNNNNTGG 1 cut(s) 1149
PkrI GCNGC 3 cut(s) 203, 1247, 1435
PleI GAGTC 1 cut(s) 1394
PpsI GAGTC 1 cut(s) 1394
Ppu21I YACGTR 1 cut(s) 1272
PscI ACATGT 1 cut(s) 759
Psp1406I AACGTT 1 cut(s) 729
Psp6I CCWGG 1 cut(s) 1351
PspFI CCCAGC 1 cut(s) 1085
PspGI CCWGG 1 cut(s) 1351
PspPI GGNCC 1 cut(s) 163
PsuI RGATCY 2 cut(s) 718, 1375
PsyI GACNNNGTC 1 cut(s) 739
RsaI GTAC 2 cut(s) 400, 1328
RsaNI GTAC 2 cut(s) 399, 1327
RseI CAYNNNNRTG 3 cut(s) 62, 459, 873
SaqAI TTAA 3 cut(s) 752, 804, 1131
SatI GCNGC 3 cut(s) 202, 1246, 1434
Sau3AI GATC 7 cut(s) 112, 368, 709, 718, 1237, 1316, 1375
Sau96I GGNCC 1 cut(s) 163
SchI GAGTC 1 cut(s) 1394
ScrFI CCNGG 2 cut(s) 106, 1353
SduI GDGCHC 3 cut(s) 59, 111, 1182
SfaNI GCATC 3 cut(s) 466, 787, 1049
SinI GGWCC 1 cut(s) 163
SmiMI CAYNNNNRTG 3 cut(s) 62, 459, 873
SmlI CTYRAG 3 cut(s) 347, 595, 923
SmoI CTYRAG 3 cut(s) 347, 595, 923
SphI GCATGC 1 cut(s) 1126
Sse9I AATT 6 cut(s) 325, 362, 559, 745, 753, 1011
SsiI CCGC 3 cut(s) 155, 564, 878
SspMI CTAG 2 cut(s) 513, 1444
StyD4I CCNGG 2 cut(s) 104, 1351
TaaI ACNGT 4 cut(s) 637, 739, 897, 1184
TaiI ACGT 2 cut(s) 732, 1274
TaqI TCGA 2 cut(s) 111, 501
TasI AATT 6 cut(s) 325, 362, 559, 745, 753, 1011
TatI WGTACW 2 cut(s) 398, 1326
TfiI GAWTC 5 cut(s) 422, 850, 1033, 1213, 1232
Tru1I TTAA 3 cut(s) 752, 804, 1131
Tru9I TTAA 3 cut(s) 752, 804, 1131
TscAI CASTG 4 cut(s) 59, 640, 1050, 1295
TseFI GTSAC 3 cut(s) 170, 643, 733
TseI GCWGC 3 cut(s) 201, 1245, 1433
Tsp45I GTSAC 3 cut(s) 170, 643, 733
TspDTI ATGAA 8 cut(s) 8, 80, 519, 827, 1005, 1311, 1370, 1400
TspGWI ACGGA 1 cut(s) 1071
TspRI CASTG 4 cut(s) 59, 640, 1050, 1295
Tth111I GACNNNGTC 1 cut(s) 739
Van91I CCANNNNNTGG 1 cut(s) 1149
VneI GTGCAC 2 cut(s) 55, 1178
VpaK11BI GGWCC 1 cut(s) 163
XceI RCATGY 3 cut(s) 660, 763, 1126
XspI CTAG 2 cut(s) 513, 1444
Zsp2I ATGCAT 1 cut(s) 1064
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.