Rh7CG046300

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0017728
Physical Location & Seq
Reverse (-)
3099356 .. 3100801
1446 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG046300.1

Sequence Viewer

Length: 1446 bp
ATGCGCAGCAACATATTTCACAAGGCACAAAGGGTCATGACCCTTTTCAAGCAGTGCACCACCATGAAAGAGGTCAAGCAACTCCATGCCCATATAACCCACACCGGGCTCGATCAAACACTCTGTGTTCTCGGCAAGGTCATCTCATTTTGCGCGGTTTCGGACCTCGGAGACATGGACTATGCGGTCTCTGTGTTCAGCAGCATTGAAAGCCCAGATGGGTTTTTATGGAACACCATGATTAGAGGGTTTGGGAAGACTGATAAGCCCGAAAGGGCGTTTGAGTTTTGTAAGAGAATGCAGGAGAAAGGAAATGTAGCTGACAATTTTACCCTCTCGTTCTTGCTCAAGGTTTCTGGGCAATTGGGATCGGTTATATTGGGGAAGCAGATACATTGTACTACTGTGAAATATGGGTTTGATTCCAATGTGTTTGTGAGGAACACTCTAATCCATATGTATGGTATGTTTGGGGATGCAAAAACTGCTCTCCACCTGTTCGATGAAATGCCTAGCCCAGATTTAGTGGCGTGGAATACCATTATTGATAGTCATGTGAATTGCGGAAAGTGCAACGAAGCGCTAGATTTGTTCTTGAGGATGATGGATAGGGGTGTAGAGCCTGACGAGGCCACTGTGGTTGTGACCCTCTCGGCATGTTCTACATTGGGTGCTTTAGATTTTGGGAGGTGGGTACATTGTTTGGTTGATCATACGGATCTTGGCAACGTTGTGACGGTGTCTAATTCGTTAATTGACATGTATGCGAAATGTGGAGCTGTTGAAGAAGCGTATGATGCTTTTAATAAGACGAAGGGGAAGAAGAACATAGTATCTTGGAACACGATGATTCTCGGGCTTGCGACGCATGGTCATGCGGACAAATCTTTGGAGCTGTTCTTGAAAATGTTGGAGGAGAAGCTTGAGAGACCGGATGGTGTTACTTTCTTGGGAGTTCTGTGTGCTTGTAGTCATGGAGGGATGGTTGATGAAGGAAGAAGATATTTTGAAATTATGAGCAAAGACTATCAGATTCAACCAACAGTGAAGCACTACGGATGCATGGTTGATATGTTGGGGCGAGCTGGGTTTGTGGAGGAGGCTTATAGGTTGATAAGGAGCATGCCGGTGAAGTGTAATGCCATTGTATGGAGGGCATTGTTGTCGGCGTGTCAGGTGCACGGTGAGGTCGAGCTTGGAGAGAAGGTCAGGAGTCATCTTTTGGAGTTGGCTTCAGATCATAGCAGCGATTATGTGCTTCTGGCTAACATGTATGCAAGTTTAGGCCAGTGGAACGAAGCTATGAGAGTAAGAAGGTCAATGCAGTACAGGGGAGTTGAGAAACCTGAGCCTGGTAATAGCTTGGTTAGCGCAGATCTTCATATGAAGTTGAAAATGGACTCAACTGAAGCCTATTCTGAAGATATAAATGCAGCAGTTTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

481

Amino Acids

53.79

Weight (kDa)

6.14

Isoelectric Point (pI)

39.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 72 - 117 4.9e-08 PPR repeat family
PPR PF01535 76 - 104 5.8e-07 PPR repeat
PPR_2 PF13041 173 - 221 6.7e-12 PPR repeat family
PPR PF01535 176 - 206 8.4e-07 PPR repeat
PPR_2 PF13041 275 - 324 9.9e-07 PPR repeat family
E_motif PF20431 394 - 456 1.6e-14 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017728)

Species Orthologous Gene IDs
malus_domestica MD06G1195900.v1.1
prunus_persica Prupe.5G195700_v2.0.a1
pyrus_communis pycom06g17310
rosa_chinensis RchiOBHm_Chr7g0181731
rosa_laevigata RLG00000005173
rosa_multiflora Rmu_sc0000988.1_g000008
rosa_roxburghii Rroxscaffold_3G00271770
rosa_rugosa Rorug06G0443000
rosa_samantha Rh7AG045600 Rh7BG044800 Rh7CG046300
rosa_wichuraiana Rw7G003800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 185
Acc16I TGCGCA 1 cut(s) 5
AccB7I CCANNNNNTGG 1 cut(s) 1149
AccII CGCG 1 cut(s) 155
AciI CCGC 4 cut(s) 155, 185, 564, 878
AclI AACGTT 1 cut(s) 729
AclWI GGATC 2 cut(s) 376, 726
AcuI CTGAAG 3 cut(s) 1218, 1428, 1440
AdeI CACNNNGTG 1 cut(s) 125
AfaI GTAC 3 cut(s) 400, 696, 1328
AfeI AGCGCT 1 cut(s) 582
AfiI CCNNNNNNNGG 3 cut(s) 105, 1149, 1352
AflIII ACRYGT 2 cut(s) 759, 1269
AgsI TTSAA 7 cut(s) 49, 209, 785, 904, 1010, 1037, 1393
AhdI GACNNNNNGTC 1 cut(s) 870
AjnI CCWGG 1 cut(s) 1351
AluBI AGCT 8 cut(s) 320, 779, 895, 922, 1085, 1195, 1301, 1362
AluI AGCT 8 cut(s) 320, 779, 895, 922, 1085, 1195, 1301, 1362
Alw21I GWGCWC 2 cut(s) 59, 1182
Alw26I GTCTC 3 cut(s) 165, 193, 922
Alw44I GTGCAC 2 cut(s) 55, 1178
AlwI GGATC 2 cut(s) 376, 726
Ama87I CYCGRG 1 cut(s) 854
Aor51HI AGCGCT 1 cut(s) 582
AoxI GGCC 2 cut(s) 630, 1285
ApaLI GTGCAC 2 cut(s) 55, 1178
ApeKI GCWGC 4 cut(s) 6, 201, 1245, 1433
AspLEI GCGC 4 cut(s) 6, 155, 583, 1373
AspS9I GGNCC 1 cut(s) 163
AsuC2I CCSGG 1 cut(s) 106
AsuHPI GGTGA 2 cut(s) 1141, 1196
AvaI CYCGRG 1 cut(s) 854
AvaII GGWCC 1 cut(s) 163
BaeGI GKGCMC 2 cut(s) 59, 1182
BanII GRGCYC 1 cut(s) 111
BbsI GAAGAC 1 cut(s) 263
Bbv12I GWGCWC 2 cut(s) 59, 1182
BbvI GCAGC 3 cut(s) 18, 213, 1257
BccI CCATC 4 cut(s) 212, 598, 929, 976
BcgI CGANNNNNNTGC 2 cut(s) 1146, 1180
BciT130I CCWGG 1 cut(s) 1353
BclI TGATCA 1 cut(s) 709
BcnI CCSGG 1 cut(s) 106
BcoDI GTCTC 3 cut(s) 165, 193, 922
BfaI CTAG 3 cut(s) 513, 584, 1444
BfoI RGCGCY 1 cut(s) 584
BglII AGATCT 1 cut(s) 1375
BisI GCNGC 4 cut(s) 7, 202, 1246, 1434
BlsI GCNGC 4 cut(s) 8, 203, 1247, 1435
Bme1390I CCNGG 2 cut(s) 106, 1353
Bme18I GGWCC 1 cut(s) 163
BmeRI GACNNNNNGTC 1 cut(s) 870
BmeT110I CYCGRG 1 cut(s) 854
BmgT120I GGNCC 1 cut(s) 163
BmrFI CCNGG 2 cut(s) 106, 1353
BmsI GCATC 3 cut(s) 466, 787, 1049
BpiI GAAGAC 1 cut(s) 263
BplI GAGNNNNNCTC 2 cut(s) 430, 462
Bpu10I CCTNAGC 1 cut(s) 1347
BpuEI CTTGAG 3 cut(s) 332, 616, 944
BpuMI CCSGG 1 cut(s) 106
BsaI GGTCTC 2 cut(s) 193, 922
BsaJI CCNNGG 1 cut(s) 166
BsaWI WCCGGW 1 cut(s) 931
BsaXI ACNNNNNCTCC 4 cut(s) 969, 999, 1191, 1221
Bsc4I CCNNNNNNNGG 3 cut(s) 105, 1149, 1352
Bse118I RCCGGY 1 cut(s) 1126
Bse1I ACTGG 1 cut(s) 1288
BseBI CCWGG 1 cut(s) 1353
BseDI CCNNGG 1 cut(s) 166
BseGI GGATG 5 cut(s) 481, 606, 940, 987, 1064
BseLI CCNNNNNNNGG 3 cut(s) 105, 1149, 1352
BseMII CTCAG 1 cut(s) 1338
BseNI ACTGG 1 cut(s) 1288
BseRI GAGGAG 2 cut(s) 929, 1112
BseSI GKGCMC 2 cut(s) 59, 1182
BseXI GCAGC 3 cut(s) 18, 213, 1257
BseYI CCCAGC 1 cut(s) 1085
Bsh1236I CGCG 1 cut(s) 155
BshFI GGCC 2 cut(s) 632, 1287
BsiHKAI GWGCWC 2 cut(s) 59, 1182
BsiHKCI CYCGRG 1 cut(s) 854
BsiSI CCGG 3 cut(s) 105, 932, 1127
BslI CCNNNNNNNGG 3 cut(s) 105, 1149, 1352
BsmAI GTCTC 3 cut(s) 165, 193, 922
BsmI GAATGC 1 cut(s) 303
BsnI GGCC 2 cut(s) 632, 1287
Bso31I GGTCTC 2 cut(s) 193, 922
BsoBI CYCGRG 1 cut(s) 854
Bsp1286I GDGCHC 3 cut(s) 59, 111, 1182
Bsp143I GATC 6 cut(s) 112, 368, 709, 718, 1237, 1375
BspACI CCGC 4 cut(s) 155, 185, 564, 878
BspANI GGCC 2 cut(s) 632, 1287
BspCNI CTCAG 1 cut(s) 1339
BspFNI CGCG 1 cut(s) 155
BspHI TCATGA 1 cut(s) 36
BspPI GGATC 2 cut(s) 376, 726
BspTNI GGTCTC 2 cut(s) 193, 922
BsrFI RCCGGY 1 cut(s) 1126
BsrI ACTGG 1 cut(s) 1288
BssAI RCCGGY 1 cut(s) 1126
BssECI CCNNGG 1 cut(s) 166
BssMI GATC 6 cut(s) 112, 368, 709, 718, 1237, 1375
Bst2UI CCWGG 1 cut(s) 1353
Bst4CI ACNGT 5 cut(s) 406, 637, 739, 1045, 1184
BstAPI GCANNNNNTGC 1 cut(s) 485
BstC8I GCNNGC 3 cut(s) 861, 1083, 1124
BstDEI CTNAG 1 cut(s) 1347
BstF5I GGATG 5 cut(s) 481, 606, 940, 987, 1064
BstFNI CGCG 1 cut(s) 155
BstH2I RGCGCY 1 cut(s) 584
BstHHI GCGC 4 cut(s) 6, 155, 583, 1373
BstKTI GATC 6 cut(s) 115, 371, 712, 721, 1240, 1378
BstMAI GTCTC 3 cut(s) 165, 193, 922
BstMBI GATC 6 cut(s) 112, 368, 709, 718, 1237, 1375
BstMWI GCNNNNNNNGC 6 cut(s) 210, 485, 570, 797, 865, 1368
BstNI CCWGG 1 cut(s) 1353
BstNSI RCATGY 4 cut(s) 660, 763, 1126, 1273
BstSCI CCNGG 2 cut(s) 104, 1351
BstSLI GKGCMC 2 cut(s) 59, 1182
BstUI CGCG 1 cut(s) 155
BstV1I GCAGC 3 cut(s) 18, 213, 1257
BstV2I GAAGAC 1 cut(s) 263
BstX2I RGATCY 2 cut(s) 718, 1375
BstXI CCANNNNNNTGG 1 cut(s) 461
BstYI RGATCY 2 cut(s) 718, 1375
BsuRI GGCC 2 cut(s) 632, 1287
BtsCI GGATG 5 cut(s) 481, 606, 940, 987, 1064
BtsI GCAGTG 1 cut(s) 59
BtsIMutI CAGTG 4 cut(s) 59, 633, 1050, 1295
Cac8I GCNNGC 3 cut(s) 861, 1083, 1124
CciI TCATGA 1 cut(s) 36
CfoI GCGC 4 cut(s) 6, 155, 583, 1373
Cfr10I RCCGGY 1 cut(s) 1126
Cfr13I GGNCC 1 cut(s) 163
CseI GACGC 1 cut(s) 874
Csp6I GTAC 3 cut(s) 399, 695, 1327
CspCI CAANNNNNGTGG 2 cut(s) 622, 657
CviQI GTAC 3 cut(s) 399, 695, 1327
DdeI CTNAG 1 cut(s) 1347
DpnI GATC 6 cut(s) 114, 370, 711, 720, 1239, 1377
DpnII GATC 6 cut(s) 112, 368, 709, 718, 1237, 1375
DraIII CACNNNGTG 1 cut(s) 125
DrdI GACNNNNNNGTC 1 cut(s) 185
DriI GACNNNNNGTC 1 cut(s) 870
DseDI GACNNNNNNGTC 1 cut(s) 185
Eam1105I GACNNNNNGTC 1 cut(s) 870
Eco24I GRGCYC 1 cut(s) 111
Eco31I GGTCTC 2 cut(s) 193, 922
Eco47I GGWCC 1 cut(s) 163
Eco47III AGCGCT 1 cut(s) 582
Eco57I CTGAAG 3 cut(s) 1218, 1428, 1440
Eco88I CYCGRG 1 cut(s) 854
EcoRII CCWGG 1 cut(s) 1351
EcoT22I ATGCAT 1 cut(s) 1064
EcoT38I GRGCYC 1 cut(s) 111
FauNDI CATATG 2 cut(s) 456, 1383
FbaI TGATCA 1 cut(s) 709
Fnu4HI GCNGC 4 cut(s) 7, 202, 1246, 1434
FokI GGATG 5 cut(s) 488, 613, 947, 994, 1071
FriOI GRGCYC 1 cut(s) 111
Fsp4HI GCNGC 4 cut(s) 7, 202, 1246, 1434
FspBI CTAG 3 cut(s) 513, 584, 1444
FspI TGCGCA 1 cut(s) 5
GlaI GCGC 4 cut(s) 5, 154, 582, 1372
GluI GCNGC 4 cut(s) 7, 202, 1246, 1434
GsaI CCCAGC 1 cut(s) 1089
HaeII RGCGCY 1 cut(s) 584
HaeIII GGCC 2 cut(s) 632, 1287
HapII CCGG 3 cut(s) 105, 932, 1127
HgaI GACGC 1 cut(s) 874
HhaI GCGC 4 cut(s) 6, 155, 583, 1373
Hin6I GCGC 4 cut(s) 4, 153, 581, 1371
HinP1I GCGC 4 cut(s) 4, 153, 581, 1371
HindIII AAGCTT 1 cut(s) 920
HinfI GANTC 5 cut(s) 422, 850, 1033, 1213, 1400
HpaII CCGG 3 cut(s) 105, 932, 1127
HphI GGTGA 2 cut(s) 1141, 1196
Hpy166II GTNNAC 3 cut(s) 57, 1180, 1441
Hpy188I TCNGA 5 cut(s) 163, 170, 1032, 1237, 1420
Hpy188III TCNNGA 4 cut(s) 37, 595, 901, 1210
Hpy8I GTNNAC 3 cut(s) 57, 1180, 1441
Hpy99I CGWCG 1 cut(s) 868
HpyAV CCTTC 4 cut(s) 808, 986, 1198, 1308
HpyCH4III ACNGT 5 cut(s) 406, 637, 739, 1045, 1184
HpyCH4IV ACGT 1 cut(s) 729
HpyCH4V TGCA 9 cut(s) 57, 301, 479, 573, 1062, 1180, 1277, 1324, 1433
HpyF10VI GCNNNNNNNGC 6 cut(s) 210, 485, 570, 797, 865, 1368
HpyF3I CTNAG 1 cut(s) 1347
HpySE526I ACGT 1 cut(s) 729
HspAI GCGC 4 cut(s) 4, 153, 581, 1371
Ksp22I TGATCA 1 cut(s) 709
Kzo9I GATC 6 cut(s) 112, 368, 709, 718, 1237, 1375
LmnI GCTCC 3 cut(s) 776, 892, 1119
Lsp1109I GCAGC 3 cut(s) 18, 213, 1257
LweI GCATC 3 cut(s) 466, 787, 1049
MaeI CTAG 3 cut(s) 513, 584, 1444
MaeII ACGT 1 cut(s) 729
MaeIII GTNAC 3 cut(s) 643, 733, 940
MalI GATC 6 cut(s) 114, 370, 711, 720, 1239, 1377
MboI GATC 6 cut(s) 112, 368, 709, 718, 1237, 1375
MboII GAAGA 8 cut(s) 268, 797, 832, 835, 1008, 1011, 1370, 1433
MfeI CAATTG 1 cut(s) 362
MflI RGATCY 2 cut(s) 718, 1375
MhlI GDGCHC 3 cut(s) 59, 111, 1182
MluCI AATT 6 cut(s) 325, 362, 559, 745, 753, 1011
MlyI GAGTC 2 cut(s) 1222, 1394
MmeI TCCRAC 1 cut(s) 891
Mph1103I ATGCAT 1 cut(s) 1064
MseI TTAA 2 cut(s) 752, 804
MslI CAYNNNNRTG 4 cut(s) 62, 459, 873, 1127
MspI CCGG 3 cut(s) 105, 932, 1127
MspR9I CCNGG 2 cut(s) 106, 1353
MunI CAATTG 1 cut(s) 362
Mva1269I GAATGC 1 cut(s) 303
MvaI CCWGG 1 cut(s) 1353
MvnI CGCG 1 cut(s) 155
MwoI GCNNNNNNNGC 6 cut(s) 210, 485, 570, 797, 865, 1368
NciI CCSGG 1 cut(s) 106
NdeI CATATG 2 cut(s) 456, 1383
NdeII GATC 6 cut(s) 112, 368, 709, 718, 1237, 1375
NmeAIII GCCGAG 2 cut(s) 111, 632
NmuCI GTSAC 2 cut(s) 643, 733
NsbI TGCGCA 1 cut(s) 5
NsiI ATGCAT 1 cut(s) 1064
NspI RCATGY 4 cut(s) 660, 763, 1126, 1273
PaeI GCATGC 1 cut(s) 1126
PagI TCATGA 1 cut(s) 36
PciI ACATGT 2 cut(s) 759, 1269
PcsI WCGNNNNNNNCGW 1 cut(s) 1188
PctI GAATGC 1 cut(s) 303
PfeI GAWTC 3 cut(s) 422, 850, 1033
PflFI GACNNNGTC 1 cut(s) 739
PflMI CCANNNNNTGG 1 cut(s) 1149
PkrI GCNGC 4 cut(s) 8, 203, 1247, 1435
PleI GAGTC 2 cut(s) 1221, 1394
PpsI GAGTC 2 cut(s) 1221, 1394
PscI ACATGT 2 cut(s) 759, 1269
Psp1406I AACGTT 1 cut(s) 729
Psp6I CCWGG 1 cut(s) 1351
PspFI CCCAGC 1 cut(s) 1085
PspGI CCWGG 1 cut(s) 1351
PspPI GGNCC 1 cut(s) 163
PsuI RGATCY 2 cut(s) 718, 1375
PsyI GACNNNGTC 1 cut(s) 739
RsaI GTAC 3 cut(s) 400, 696, 1328
RsaNI GTAC 3 cut(s) 399, 695, 1327
RseI CAYNNNNRTG 4 cut(s) 62, 459, 873, 1127
SaqAI TTAA 2 cut(s) 752, 804
SatI GCNGC 4 cut(s) 7, 202, 1246, 1434
Sau3AI GATC 6 cut(s) 112, 368, 709, 718, 1237, 1375
Sau96I GGNCC 1 cut(s) 163
SchI GAGTC 2 cut(s) 1222, 1394
ScrFI CCNGG 2 cut(s) 106, 1353
SduI GDGCHC 3 cut(s) 59, 111, 1182
SfaNI GCATC 3 cut(s) 466, 787, 1049
SinI GGWCC 1 cut(s) 163
SmiMI CAYNNNNRTG 4 cut(s) 62, 459, 873, 1127
SmlI CTYRAG 3 cut(s) 347, 595, 923
SmoI CTYRAG 3 cut(s) 347, 595, 923
SphI GCATGC 1 cut(s) 1126
Sse9I AATT 6 cut(s) 325, 362, 559, 745, 753, 1011
SsiI CCGC 4 cut(s) 155, 185, 564, 878
SspMI CTAG 3 cut(s) 513, 584, 1444
StyD4I CCNGG 2 cut(s) 104, 1351
TaaI ACNGT 5 cut(s) 406, 637, 739, 1045, 1184
TaiI ACGT 1 cut(s) 732
TaqI TCGA 3 cut(s) 111, 501, 1191
TasI AATT 6 cut(s) 325, 362, 559, 745, 753, 1011
TatI WGTACW 2 cut(s) 398, 1326
TfiI GAWTC 3 cut(s) 422, 850, 1033
Tru1I TTAA 2 cut(s) 752, 804
Tru9I TTAA 2 cut(s) 752, 804
TscAI CASTG 4 cut(s) 59, 640, 1050, 1295
TseFI GTSAC 2 cut(s) 643, 733
TseI GCWGC 4 cut(s) 6, 201, 1245, 1433
Tsp45I GTSAC 2 cut(s) 643, 733
TspDTI ATGAA 5 cut(s) 80, 519, 1005, 1370, 1400
TspGWI ACGGA 2 cut(s) 731, 1071
TspRI CASTG 4 cut(s) 59, 640, 1050, 1295
Tth111I GACNNNGTC 1 cut(s) 739
Van91I CCANNNNNTGG 1 cut(s) 1149
VneI GTGCAC 2 cut(s) 55, 1178
VpaK11BI GGWCC 1 cut(s) 163
XceI RCATGY 4 cut(s) 660, 763, 1126, 1273
XspI CTAG 3 cut(s) 513, 584, 1444
Zsp2I ATGCAT 1 cut(s) 1064
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.