MD06G1199300.v1.1

Plant calmodulin-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0012620
Physical Location & Seq
Forward (+)
33303074 .. 33308103
5030 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1199300.v1.1.491

Sequence Viewer

Length: 3273 bp
ATGGAAGCAGAGAACATCGGTATCCCAGTGATCCTACAAGGCCTGGACCTTCTTGGTGAAAAGGCCAGAAAGAATTCGATCCCACAGGAAGCGGAGTCCAATGGTGGTGACGTTGGCAGTGATATCAGAAGGGATTCCATCGCGGAGATCCCACCAGAAATGGATCCCAAGGGTGGTGATGTCAGGGGTAACTCTGATCCAGGGGTTTCACAGGACATAGAGCCCAATGCCGGGGATAGCAGAAGGAGCTCTATCGGAAATGTTGATCCTCCTCATATCAAAAGGAGCTCTATCCCAGTGATCCCACAGGAGATGGATCCCAAGGGTGGTGATGTCGGAGGTAATCCAGTCGTGGGGATTCCACAGGACATAGAGCTCGATAGAGGTGACATCAGAAGGAGCTCTATCGGAAATCTTGATCCTCCTTCTACCGAGAGGAACTCTATCCCAGTGGTCCTGCAGGACATGGATCTCAGTGATGATGATAGGAACTTTATTGCAGCTGTCCCCCAGGATATAGAACCCGATGGCAGTGATATCAAACAGAAATCTATCCTAGCAATCCCACACAAAATGGAGCATGAGGGTGGTGATATCAGAAGGAAGCCTATCCCAGTGAGGCCACGGGAAATAGAGTCTAATGCCGGTGATATCAGAAGAAACTCCATAGAGAAAGTAGATCCTCCTGCTGATATCAAAAGGAACTCTATCCCAGTGATCCCACAGGAAATGAAGCCTAATGCTGGTGATGTTAGAAGGAACTCTATTGGAGTGATCCCACAGGAATTAGAGCACAGTGGTGGTGGTATCAGAAGGAACTCTATGCCAGAGGTCCCACAGAAAATGGAACCAAAGGCTGGCGATACCAGCAAGAACTCTGTTCTGTGGATCCCACATGAAGTGGAACCAAAGGGTGGTAGATGGATTCAGTCCCGATATCTTAGAGCTCCAATAAGTTCCTGCCATGATTATTGCAAATATGGAGTGAGAAATGCCTCTGAAGAAAATGCAACTGGCGCACTGCGAAAATCAGCTACAGAGAGGAAACGGATATCCACAACCAGTCAAAACCCTTCTCCAGATTCAGACACCCAGAAGCTTGACAATTCTGTCGTCACAGAGAAAAAAGTATTGTCATTGACAAAGAAAGAAATTGTTTCTGCAGAAAATGTTCCATCTTCTTCCAAGGACATTGATGTTTCTATGGAAGATTCCGCCGGTTTGAAGGTGAAACGTGTGCAATCAGAGCCATCTTCTCTCCCAGAAGCGGCCTTAAGCCTTAGAAATTCCAAGGTGATATCCACAACTGGACCGAGGTCTGTTCCACATTCCCCAACCCGGAAACTTAATAATTATGTTGTCACGGAGAAAAAAGGTTCATCGTCGACAAAAGAAACTTCTTCAAAGAAAGTTTCATCTCCCAAGGAAATAGCTGTTTGTATGGAAGAGTTGATTGATTTGAAGATGAAGGATAAGCAATTAGATCAATCTTCTCTCCCAGGATCAGACCCAAGCTTGAGAAATTCGAAGGTAACATCAACAATCAATCCTCACCCTTCTCCAGATTCTGAGTCCCAGAGTTCTACTGCCACGGAGAAAAGAGTTTCACCTCCCTATAAGGATATAGATGTTTCTGCTGAAGATTCCACCGATTCAAAGGTGATATCAACAGTCAGTCCTAATCCTTCTCCAGATTCTGAAGCACAGGAACTTAATCATTCTGGTGGCACGGAGAAAAGAGTTTTATCATCGAAAAGGAAAGAAAATGTCTCTTCAAAAACAGTTTTGACTCCCATTAAGGAGATTGATGTTTCTACGGAAGGTTCCGCTTGTTCAAAGGTGAAGCCTGAGCAACTAGAGCCATCTTCTCGCACAGGACAAGGTTCAAGTAATCAAGGAAAGAGTGGAACTAGAAAAGGCAAGGAAATACAAGAAGGTTCGAGCAGTAGTGTAAATAGAAAAATCAGAAGCAAGCTGCAAAGGACTCCTGTATTGGTTGAGAAAAAGATGTTAGAGCCTGAAACTGTTTCTTTGACTTCCAAACACCCTGTCAAGAGAGTCTCCGATGCAAATGCTGGAAGATCTAAGAACCTTAAAGTATTGTCTCATATGAAAGATCAAAAGTGTCCCCGAAAAGTTGAACCTGAGGGACCCTGCAATAAGGATGTTCCAGAAAAGATTTTGTATGTCGTCAAATCTAATACTGGGAATAGTACTGTCAAACCAACTCCAAATGGTGCTGATGCCCCTGAGTTATCATCATCATCACATCTATCCTCTGATGTCAAAAGCTCGAGGCAAGCAAGGAAGGGAGTTGGTACCACTCGATCACATACATCCTCCAAGAAGAAAAACTTCAAACGCACTGTAAATGGAGGTTATGGTCGTTCAACTACTGCTCCACCCCCTGAAAACAAAGGTTTGAGGCGGAGTACTCGAAGACCTTTATCTTCATTGTCATCGTCATCATCATCATCCATGTCCGCATCCCCAGGCCAGTCCACTCATAAAAGAGAAAATGGCGCCACCTCTCAATGCGGAGCAATGAAAACTGGTAATCAAAGAGAAAATTTGAAGGTGGAGAAGAGTACTAGGCCTAGAAAGTCTGAGACACATCACCCTGAAAGTAAAAACAGCTCAGCCCGAAAGCTGACATTTAAGAGAGGAAGGGTGCTTGACATAAAGCCAGAGATCAGTACTACAAGGAGACTCAAATTCAGGCGAGTAAGATTGGCAGGGGAGATCCAAAATGGTAAAGGTGGTGTCACAGGGGGGAGCGCCGGGAGGAAAGAAGTTTGTGACAACCATTCAGTTGGTGATGTATTAAAACAGTTAAGCCCCGGGAGGAAAGTAGCTGATCACAGTCACTCAAATGGTGCTTTCATCAAAAGGCGAAGCCCCGGGACGAAAGAAGTTGATGGCAGCCAGTCAAATGGTAGTAAATTCAAAAGTGAGAAATTTGTTCTGAGAAGCTTCGAACGAGATGTGGAAGGTTCACGAAGGAAGAGCTCGAGGAGGAAAACGGCTAGTGATAGCGGGTTGAATGGTAGTACTAGAACGGAACCTGAGAGGGTTGTGTTGAGACATCAAGATGTGAAAGGAAGGAAGGATGTGCAGAAGTTGTTAAATAATGTGATCGAAGAGACTGCCAGTAGGCTTGTTGTGTCCAGGAAGAGTAAAGTCAAGGCCTTGGTTGGTGCTTTTGAGACCGTGATTTCGCGTCAGGATACCAAATCCATGGCTTCGGAAACAGACACGGAAGATGCAGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaM_binding

Protein Analysis

1091

Amino Acids

118.38

Weight (kDa)

9.67

Isoelectric Point (pI)

64.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CaM_binding PF07839 976 - 1073 1.7e-26 Plant calmodulin-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1109
Acc65I GGTACC 1 cut(s) 2318
AccB1I GGYRCC 2 cut(s) 2318, 2522
AccB7I CCANNNNNTGG 2 cut(s) 857, 914
AccI GTMKAC 1 cut(s) 1385
AccII CGCG 2 cut(s) 143, 3220
AciI CCGC 9 cut(s) 92, 143, 1215, 1268, 1827, 2428, 2484, 2538, 3036
AcsI RAATTY 7 cut(s) 73, 1285, 1522, 2569, 2714, 2942, 2957
AcuI CTGAAG 3 cut(s) 1020, 1659, 1719
AcyI GRCGYC 1 cut(s) 2523
AfaI GTAC 6 cut(s) 2215, 2320, 2434, 2590, 2698, 3052
AflII CTTAAG 1 cut(s) 1273
AflIII ACRYGT 1 cut(s) 1234
AjnI CCWGG 6 cut(s) 42, 199, 510, 1498, 2491, 3167
AleI CACNNNNGTG 1 cut(s) 798
Alw21I GWGCWC 7 cut(s) 251, 290, 378, 404, 795, 949, 3011
Alw26I GTCTC 8 cut(s) 1774, 2065, 2109, 2603, 2701, 3076, 3137, 3200
AlwNI CAGNNNCTG 2 cut(s) 1568, 1697
Ama87I CYCGRG 4 cut(s) 2293, 2840, 2900, 3010
AoxI GGCC 7 cut(s) 40, 63, 620, 1269, 2494, 2594, 3186
ApeKI GCWGC 3 cut(s) 500, 1975, 2922
ApoI RAATTY 7 cut(s) 73, 1285, 1522, 2569, 2714, 2942, 2957
ArsI GACNNNNNNTTYG 4 cut(s) 2034, 2066, 3199, 3231
Asp700I GAANNNNTTC 4 cut(s) 73, 133, 1170, 2354
Asp718I GGTACC 1 cut(s) 2318
AspLEI GCGC 3 cut(s) 1019, 2525, 2780
AspS9I GGNCC 5 cut(s) 46, 454, 832, 1310, 2150
AsuC2I CCSGG 7 cut(s) 232, 1339, 2782, 2841, 2842, 2901, 2902
AsuII TTCGAA 2 cut(s) 1526, 2976
AvaI CYCGRG 4 cut(s) 2293, 2840, 2900, 3010
AvaII GGWCC 5 cut(s) 46, 454, 832, 1310, 2150
AxyI CCTNAGG 1 cut(s) 2145
BaeI ACNNNNGTAYC 1 cut(s) 37
BamHI GGATCC 3 cut(s) 163, 316, 888
BanI GGYRCC 2 cut(s) 2318, 2522
BanII GRGCYC 7 cut(s) 225, 251, 290, 378, 404, 949, 3011
BbsI GAAGAC 1 cut(s) 2446
Bbv12I GWGCWC 7 cut(s) 251, 290, 378, 404, 795, 949, 3011
BbvI GCAGC 3 cut(s) 512, 1962, 2934
BccI CCATC 8 cut(s) 146, 307, 521, 915, 1183, 1258, 1870, 2912
BceAI ACGGC 1 cut(s) 3039
BcgI CGANNNNNNTGC 4 cut(s) 2054, 2088, 3128, 3162
BciT130I CCWGG 6 cut(s) 44, 201, 512, 1500, 2493, 3169
BciVI GTATCC 2 cut(s) 32, 3220
BclI TGATCA 1 cut(s) 2857
BcnI CCSGG 7 cut(s) 232, 1339, 2782, 2841, 2842, 2901, 2902
BcoDI GTCTC 8 cut(s) 1774, 2065, 2109, 2603, 2701, 3076, 3137, 3200
BfaI CTAG 7 cut(s) 557, 1856, 1911, 2592, 2598, 3027, 3054
BfmI CTRYAG 3 cut(s) 458, 1035, 1161
BfoI RGCGCY 2 cut(s) 2526, 2781
BfrI CTTAAG 1 cut(s) 1273
BfuI GTATCC 2 cut(s) 32, 3220
BglII AGATCT 1 cut(s) 2081
BisI GCNGC 4 cut(s) 501, 1269, 1976, 2923
BlpI GCTNAGC 1 cut(s) 2638
BlsI GCNGC 4 cut(s) 502, 1270, 1977, 2924
BmcAI AGTACT 5 cut(s) 2215, 2434, 2590, 2698, 3052
Bme18I GGWCC 5 cut(s) 46, 454, 832, 1310, 2150
BmeT110I CYCGRG 4 cut(s) 2293, 2840, 2900, 3010
BmgT120I GGNCC 5 cut(s) 46, 454, 832, 1310, 2150
BmrI ACTGGG 6 cut(s) 20, 290, 443, 608, 707, 2214
BmsI GCATC 4 cut(s) 2056, 2233, 2495, 3253
BmuI ACTGGG 6 cut(s) 20, 290, 443, 608, 707, 2214
BoxI GACNNNNGTC 1 cut(s) 1315
BpiI GAAGAC 1 cut(s) 2446
BplI GAGNNNNNCTC 2 cut(s) 425, 457
BpmI CTGGAG 3 cut(s) 1062, 1545, 1674
Bpu10I CCTNAGC 1 cut(s) 1848
Bpu1102I GCTNAGC 1 cut(s) 2638
Bpu14I TTCGAA 2 cut(s) 1526, 2976
BpuEI CTTGAG 1 cut(s) 1537
BpuMI CCSGG 7 cut(s) 232, 1339, 2782, 2841, 2842, 2901, 2902
BsaHI GRCGYC 1 cut(s) 2523
BsaI GGTCTC 1 cut(s) 3200
BsaXI ACNNNNNCTCC 2 cut(s) 2383, 2413
Bse118I RCCGGY 2 cut(s) 644, 1217
Bse21I CCTNAGG 1 cut(s) 2145
Bse3DI GCAATG 1 cut(s) 2550
BseBI CCWGG 6 cut(s) 44, 201, 512, 1500, 2493, 3169
BseGI GGATG 5 cut(s) 2170, 2336, 2474, 2486, 3115
BseMI GCAATG 1 cut(s) 2550
BseMII CTCAG 9 cut(s) 487, 1560, 1839, 2136, 2241, 2598, 2652, 2957, 3057
BseRI GAGGAG 2 cut(s) 261, 3028
BseXI GCAGC 3 cut(s) 512, 1962, 2934
BsgI GTGCAG 1 cut(s) 3134
Bsh1236I CGCG 2 cut(s) 143, 3220
BshFI GGCC 7 cut(s) 42, 65, 622, 1271, 2496, 2596, 3188
BshNI GGYRCC 2 cut(s) 2318, 2522
BsiHKAI GWGCWC 7 cut(s) 251, 290, 378, 404, 795, 949, 3011
BsiHKCI CYCGRG 4 cut(s) 2293, 2840, 2900, 3010
BsiSI CCGG 7 cut(s) 231, 645, 1218, 1339, 2781, 2841, 2901
BslFI GGGAC 7 cut(s) 491, 818, 916, 1558, 2112, 2163, 2917
BsmAI GTCTC 8 cut(s) 1774, 2065, 2109, 2603, 2701, 3076, 3137, 3200
BsmFI GGGAC 7 cut(s) 491, 818, 916, 1558, 2112, 2163, 2917
BsnI GGCC 7 cut(s) 42, 65, 622, 1271, 2496, 2596, 3188
Bso31I GGTCTC 1 cut(s) 3200
BsoBI CYCGRG 4 cut(s) 2293, 2840, 2900, 3010
Bsp119I TTCGAA 2 cut(s) 1526, 2976
Bsp1286I GDGCHC 8 cut(s) 225, 251, 290, 378, 404, 795, 949, 3011
Bsp1720I GCTNAGC 1 cut(s) 2638
Bsp19I CCATGG 1 cut(s) 3237
BspACI CCGC 9 cut(s) 92, 143, 1215, 1268, 1827, 2428, 2484, 2538, 3036
BspANI GGCC 7 cut(s) 42, 65, 622, 1271, 2496, 2596, 3188
BspCNI CTCAG 9 cut(s) 486, 1561, 1840, 2137, 2242, 2599, 2651, 2958, 3058
BspFNI CGCG 2 cut(s) 143, 3220
BspMAI CTGCAG 2 cut(s) 462, 1165
BspQI GCTCTTC 1 cut(s) 2999
BspT104I TTCGAA 2 cut(s) 1526, 2976
BspT107I GGYRCC 2 cut(s) 2318, 2522
BspTI CTTAAG 1 cut(s) 1273
BspTNI GGTCTC 1 cut(s) 3200
BsrDI GCAATG 1 cut(s) 2550
BsrFI RCCGGY 2 cut(s) 644, 1217
BssAI RCCGGY 2 cut(s) 644, 1217
BssNI GRCGYC 1 cut(s) 2523
BssT1I CCWWGG 7 cut(s) 168, 321, 1185, 1290, 1422, 3189, 3237
Bst2UI CCWGG 6 cut(s) 44, 201, 512, 1500, 2493, 3169
Bst4CI ACNGT 9 cut(s) 797, 1672, 1783, 2026, 2218, 2368, 2832, 2864, 3211
Bst6I CTCTTC 6 cut(s) 1440, 1777, 2579, 2999, 3135, 3167
BstACI GRCGYC 1 cut(s) 2523
BstAFI CTTAAG 1 cut(s) 1273
BstBI TTCGAA 2 cut(s) 1526, 2976
BstC8I GCNNGC 3 cut(s) 859, 1973, 2301
BstDSI CCRYGG 3 cut(s) 623, 1590, 3237
BstF5I GGATG 5 cut(s) 2170, 2336, 2474, 2486, 3115
BstFNI CGCG 2 cut(s) 143, 3220
BstH2I RGCGCY 2 cut(s) 2526, 2781
BstHHI GCGC 3 cut(s) 1019, 2525, 2780
BstMAI GTCTC 8 cut(s) 1774, 2065, 2109, 2603, 2701, 3076, 3137, 3200
BstMWI GCNNNNNNNGC 5 cut(s) 246, 867, 1016, 1246, 1858
BstNI CCWGG 6 cut(s) 44, 201, 512, 1500, 2493, 3169
BstPAI GACNNNNGTC 1 cut(s) 1315
BstSFI CTRYAG 3 cut(s) 458, 1035, 1161
BstUI CGCG 2 cut(s) 143, 3220
BstV1I GCAGC 3 cut(s) 512, 1962, 2934
BstV2I GAAGAC 1 cut(s) 2446
BstX2I RGATCY 8 cut(s) 147, 163, 316, 469, 679, 888, 2081, 2742
BstXI CCANNNNNNTGG 3 cut(s) 2813, 2933, 3238
BstYI RGATCY 8 cut(s) 147, 163, 316, 469, 679, 888, 2081, 2742
Bsu36I CCTNAGG 1 cut(s) 2145
BsuI GTATCC 2 cut(s) 32, 3220
BsuRI GGCC 7 cut(s) 42, 65, 622, 1271, 2496, 2596, 3188
BtgI CCRYGG 3 cut(s) 623, 1590, 3237
BtgZI GCGATG 1 cut(s) 124
BtsCI GGATG 5 cut(s) 2170, 2336, 2474, 2486, 3115
BtsI GCAGTG 3 cut(s) 124, 538, 1019
Cac8I GCNNGC 3 cut(s) 859, 1973, 2301
CaiI CAGNNNCTG 2 cut(s) 1568, 1697
CfoI GCGC 3 cut(s) 1019, 2525, 2780
Cfr10I RCCGGY 2 cut(s) 644, 1217
Cfr13I GGNCC 5 cut(s) 46, 454, 832, 1310, 2150
Cfr9I CCCGGG 2 cut(s) 2840, 2900
CseI GACGC 1 cut(s) 3209
Csp6I GTAC 6 cut(s) 2214, 2319, 2433, 2589, 2697, 3051
CviAII CATG 6 cut(s) 466, 581, 896, 965, 2479, 3238
CviQI GTAC 6 cut(s) 2214, 2319, 2433, 2589, 2697, 3051
DinI GGCGCC 1 cut(s) 2524
DrdI GACNNNNNNGTC 1 cut(s) 1109
DseDI GACNNNNNNGTC 1 cut(s) 1109
Eam1104I CTCTTC 6 cut(s) 1440, 1777, 2579, 2999, 3135, 3167
EarI CTCTTC 6 cut(s) 1440, 1777, 2579, 2999, 3135, 3167
EciI GGCGGA 2 cut(s) 1204, 2443
Ecl136II GAGCTC 6 cut(s) 249, 288, 376, 402, 947, 3009
Eco130I CCWWGG 7 cut(s) 168, 321, 1185, 1290, 1422, 3189, 3237
Eco147I AGGCCT 3 cut(s) 42, 2596, 3188
Eco24I GRGCYC 7 cut(s) 225, 251, 290, 378, 404, 949, 3011
Eco31I GGTCTC 1 cut(s) 3200
Eco32I GATATC 9 cut(s) 124, 538, 595, 652, 694, 938, 1053, 1299, 1665
Eco47I GGWCC 5 cut(s) 46, 454, 832, 1310, 2150
Eco53kI GAGCTC 6 cut(s) 249, 288, 376, 402, 947, 3009
Eco57I CTGAAG 3 cut(s) 1020, 1659, 1719
Eco81I CCTNAGG 1 cut(s) 2145
Eco88I CYCGRG 4 cut(s) 2293, 2840, 2900, 3010
EcoICRI GAGCTC 6 cut(s) 249, 288, 376, 402, 947, 3009
EcoO109I RGGNCCY 2 cut(s) 832, 2150
EcoRI GAATTC 1 cut(s) 73
EcoRII CCWGG 6 cut(s) 42, 199, 510, 1498, 2491, 3167
EcoRV GATATC 9 cut(s) 124, 538, 595, 652, 694, 938, 1053, 1299, 1665
EcoT14I CCWWGG 7 cut(s) 168, 321, 1185, 1290, 1422, 3189, 3237
EcoT38I GRGCYC 7 cut(s) 225, 251, 290, 378, 404, 949, 3011
EgeI GGCGCC 1 cut(s) 2524
EheI GGCGCC 1 cut(s) 2524
ErhI CCWWGG 7 cut(s) 168, 321, 1185, 1290, 1422, 3189, 3237
FaeI CATG 6 cut(s) 469, 584, 899, 968, 2482, 3241
FalI AAGNNNNNCTT 2 cut(s) 2339, 2371
FaqI GGGAC 7 cut(s) 491, 818, 916, 1558, 2112, 2163, 2917
FatI CATG 6 cut(s) 465, 580, 895, 964, 2478, 3237
FauI CCCGC 1 cut(s) 3029
FauNDI CATATG 1 cut(s) 2109
FbaI TGATCA 1 cut(s) 2857
FblI GTMKAC 1 cut(s) 1385
Fnu4HI GCNGC 4 cut(s) 501, 1269, 1976, 2923
FokI GGATG 5 cut(s) 2177, 2323, 2461, 2473, 3122
FriOI GRGCYC 7 cut(s) 225, 251, 290, 378, 404, 949, 3011
Fsp4HI GCNGC 4 cut(s) 501, 1269, 1976, 2923
FspBI CTAG 7 cut(s) 557, 1856, 1911, 2592, 2598, 3027, 3054
GlaI GCGC 3 cut(s) 1018, 2524, 2779
GluI GCNGC 4 cut(s) 501, 1269, 1976, 2923
GsuI CTGGAG 3 cut(s) 1062, 1545, 1674
HaeII RGCGCY 2 cut(s) 2526, 2781
HaeIII GGCC 7 cut(s) 42, 65, 622, 1271, 2496, 2596, 3188
HapII CCGG 7 cut(s) 231, 645, 1218, 1339, 2781, 2841, 2901
HgaI GACGC 1 cut(s) 3209
HhaI GCGC 3 cut(s) 1019, 2525, 2780
Hin1I GRCGYC 1 cut(s) 2523
Hin1II CATG 6 cut(s) 469, 584, 899, 968, 2482, 3241
Hin6I GCGC 3 cut(s) 1017, 2523, 2778
HinP1I GCGC 3 cut(s) 1017, 2523, 2778
HincII GTYRAC 1 cut(s) 1386
HindII GTYRAC 1 cut(s) 1386
HindIII AAGCTT 3 cut(s) 1097, 1513, 2971
HpaII CCGG 7 cut(s) 231, 645, 1218, 1339, 2781, 2841, 2901
Hpy166II GTNNAC 3 cut(s) 1386, 2502, 2996
Hpy8I GTNNAC 3 cut(s) 1386, 2502, 2996
Hpy99I CGWCG 1 cut(s) 1387
HpyCH4III ACNGT 9 cut(s) 797, 1672, 1783, 2026, 2218, 2368, 2832, 2864, 3211
HpyCH4IV ACGT 2 cut(s) 111, 1234
HpyF10VI GCNNNNNNNGC 5 cut(s) 246, 867, 1016, 1246, 1858
HpySE526I ACGT 2 cut(s) 111, 1234
Hsp92I GRCGYC 1 cut(s) 2523
Hsp92II CATG 6 cut(s) 469, 584, 899, 968, 2482, 3241
HspAI GCGC 3 cut(s) 1017, 2523, 2778
KasI GGCGCC 1 cut(s) 2522
KflI GGGWCCC 1 cut(s) 2150
KpnI GGTACC 1 cut(s) 2322
Ksp22I TGATCA 1 cut(s) 2857
LguI GCTCTTC 1 cut(s) 2999
LmnI GCTCC 8 cut(s) 246, 285, 399, 577, 952, 2404, 2540, 2775
Lsp1109I GCAGC 3 cut(s) 512, 1962, 2934
LweI GCATC 4 cut(s) 2056, 2233, 2495, 3253
MaeI CTAG 7 cut(s) 557, 1856, 1911, 2592, 2598, 3027, 3054
MaeII ACGT 2 cut(s) 111, 1234
MaeIII GTNAC 9 cut(s) 107, 188, 386, 1114, 1360, 1531, 2764, 2798, 2864
MflI RGATCY 8 cut(s) 147, 163, 316, 469, 679, 888, 2081, 2742
MhlI GDGCHC 8 cut(s) 225, 251, 290, 378, 404, 795, 949, 3011
Mly113I GGCGCC 1 cut(s) 2523
MlyI GAGTC 7 cut(s) 104, 644, 1580, 1783, 1978, 2067, 2703
MmeI TCCRAC 1 cut(s) 316
MroXI GAANNNNTTC 4 cut(s) 73, 133, 1170, 2354
MseI TTAA 9 cut(s) 1274, 1347, 1713, 1797, 2094, 2658, 2825, 2834, 3125
MslI CAYNNNNRTG 5 cut(s) 585, 798, 1722, 2871, 3090
MspA1I CMGCKG 1 cut(s) 503
MspCI CTTAAG 1 cut(s) 1273
MspI CCGG 7 cut(s) 231, 645, 1218, 1339, 2781, 2841, 2901
MvaI CCWGG 6 cut(s) 44, 201, 512, 1500, 2493, 3169
MvnI CGCG 2 cut(s) 143, 3220
MwoI GCNNNNNNNGC 5 cut(s) 246, 867, 1016, 1246, 1858
NarI GGCGCC 1 cut(s) 2523
NciI CCSGG 7 cut(s) 232, 1339, 2782, 2841, 2842, 2901, 2902
NcoI CCATGG 1 cut(s) 3237
NdeI CATATG 1 cut(s) 2109
NlaIII CATG 6 cut(s) 469, 584, 899, 968, 2482, 3241
NmuCI GTSAC 7 cut(s) 107, 386, 1114, 1360, 2764, 2798, 2864
NspV TTCGAA 2 cut(s) 1526, 2976
OliI CACNNNNGTG 1 cut(s) 798
PaeR7I CTCGAG 2 cut(s) 2293, 3010
PceI AGGCCT 3 cut(s) 42, 2596, 3188
PciSI GCTCTTC 1 cut(s) 2999
PdmI GAANNNNTTC 4 cut(s) 73, 133, 1170, 2354
PfeI GAWTC 9 cut(s) 134, 358, 925, 1082, 1211, 1565, 1643, 1652, 1694
PflMI CCANNNNNTGG 2 cut(s) 857, 914
PfoI TCCNGGA 1 cut(s) 3167
PkrI GCNGC 4 cut(s) 502, 1270, 1977, 2924
PleI GAGTC 7 cut(s) 103, 643, 1579, 1783, 1978, 2066, 2703
PluTI GGCGCC 1 cut(s) 2526
PpsI GAGTC 7 cut(s) 103, 643, 1579, 1783, 1978, 2066, 2703
PpuMI RGGWCCY 2 cut(s) 832, 2150
PshAI GACNNNNGTC 1 cut(s) 1315
Psp124BI GAGCTC 6 cut(s) 251, 290, 378, 404, 949, 3011
Psp5II RGGWCCY 2 cut(s) 832, 2150
Psp6I CCWGG 6 cut(s) 42, 199, 510, 1498, 2491, 3167
PspGI CCWGG 6 cut(s) 42, 199, 510, 1498, 2491, 3167
PspPI GGNCC 5 cut(s) 46, 454, 832, 1310, 2150
PspPPI RGGWCCY 2 cut(s) 832, 2150
PspXI VCTCGAGB 2 cut(s) 2293, 3010
PstI CTGCAG 2 cut(s) 462, 1165
PstNI CAGNNNCTG 2 cut(s) 1568, 1697
PsuI RGATCY 8 cut(s) 147, 163, 316, 469, 679, 888, 2081, 2742
PvuII CAGCTG 1 cut(s) 503
RsaI GTAC 6 cut(s) 2215, 2320, 2434, 2590, 2698, 3052
RsaNI GTAC 6 cut(s) 2214, 2319, 2433, 2589, 2697, 3051
RseI CAYNNNNRTG 5 cut(s) 585, 798, 1722, 2871, 3090
SacI GAGCTC 6 cut(s) 251, 290, 378, 404, 949, 3011
SalI GTCGAC 1 cut(s) 1384
SapI GCTCTTC 1 cut(s) 2999
SaqAI TTAA 9 cut(s) 1274, 1347, 1713, 1797, 2094, 2658, 2825, 2834, 3125
SatI GCNGC 4 cut(s) 501, 1269, 1976, 2923
Sau96I GGNCC 5 cut(s) 46, 454, 832, 1310, 2150
SbfI CCTGCAGG 1 cut(s) 462
ScaI AGTACT 5 cut(s) 2215, 2434, 2590, 2698, 3052
SchI GAGTC 7 cut(s) 104, 644, 1580, 1783, 1978, 2067, 2703
SdaI CCTGCAGG 1 cut(s) 462
SduI GDGCHC 8 cut(s) 225, 251, 290, 378, 404, 795, 949, 3011
SfaNI GCATC 4 cut(s) 2056, 2233, 2495, 3253
SfcI CTRYAG 3 cut(s) 458, 1035, 1161
SfoI GGCGCC 1 cut(s) 2524
Sfr274I CTCGAG 2 cut(s) 2293, 3010
SfuI TTCGAA 2 cut(s) 1526, 2976
SinI GGWCC 5 cut(s) 46, 454, 832, 1310, 2150
SlaI CTCGAG 2 cut(s) 2293, 3010
SmaI CCCGGG 2 cut(s) 2842, 2902
SmiMI CAYNNNNRTG 5 cut(s) 585, 798, 1722, 2871, 3090
SmlI CTYRAG 4 cut(s) 1273, 1516, 2293, 3010
SmoI CTYRAG 4 cut(s) 1273, 1516, 2293, 3010
Sse8387I CCTGCAGG 1 cut(s) 462
SseBI AGGCCT 3 cut(s) 42, 2596, 3188
SsiI CCGC 9 cut(s) 92, 143, 1215, 1268, 1827, 2428, 2484, 2538, 3036
SspDI GGCGCC 1 cut(s) 2522
SspMI CTAG 7 cut(s) 557, 1856, 1911, 2592, 2598, 3027, 3054
SstI GAGCTC 6 cut(s) 251, 290, 378, 404, 949, 3011
StuI AGGCCT 3 cut(s) 42, 2596, 3188
StyI CCWWGG 7 cut(s) 168, 321, 1185, 1290, 1422, 3189, 3237
TaaI ACNGT 9 cut(s) 797, 1672, 1783, 2026, 2218, 2368, 2832, 2864, 3211
TaiI ACGT 2 cut(s) 114, 1237
TaqII GACCGA 1 cut(s) 1327
TatI WGTACW 5 cut(s) 2213, 2432, 2588, 2696, 3050
TauI GCSGC 1 cut(s) 1271
TfiI GAWTC 9 cut(s) 134, 358, 925, 1082, 1211, 1565, 1643, 1652, 1694
Tru1I TTAA 9 cut(s) 1274, 1347, 1713, 1797, 2094, 2658, 2825, 2834, 3125
Tru9I TTAA 9 cut(s) 1274, 1347, 1713, 1797, 2094, 2658, 2825, 2834, 3125
TseFI GTSAC 7 cut(s) 107, 386, 1114, 1360, 2764, 2798, 2864
TseI GCWGC 3 cut(s) 500, 1975, 2922
Tsp45I GTSAC 7 cut(s) 107, 386, 1114, 1360, 2764, 2798, 2864
TspDTI ATGAA 9 cut(s) 746, 912, 1368, 1404, 1481, 2126, 2442, 2561, 2872
TspGWI ACGGA 7 cut(s) 1063, 1379, 1607, 1745, 1832, 3074, 3272
TspMI CCCGGG 2 cut(s) 2840, 2900
Van91I CCANNNNNTGG 2 cut(s) 857, 914
Vha464I CTTAAG 1 cut(s) 1273
VpaK11BI GGWCC 5 cut(s) 46, 454, 832, 1310, 2150
XapI RAATTY 7 cut(s) 73, 1285, 1522, 2569, 2714, 2942, 2957
XhoI CTCGAG 2 cut(s) 2293, 3010
XmaI CCCGGG 2 cut(s) 2840, 2900
XmiI GTMKAC 1 cut(s) 1385
XmnI GAANNNNTTC 4 cut(s) 73, 133, 1170, 2354
XspI CTAG 7 cut(s) 557, 1856, 1911, 2592, 2598, 3027, 3054
ZrmI AGTACT 5 cut(s) 2215, 2434, 2590, 2698, 3052
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.