RLG00000005130

Plant calmodulin-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0012620
Physical Location & Seq
Reverse (-)
65240736 .. 65245046
4311 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005130

Sequence Viewer

Length: 3174 bp
ATGGAGAGACGGCTGTGCCCCCTATATACTTGGCGCGTTTTTGTCGTGGAATCACAGCCACCAATAAATCCCCTTTTGGGGTGTTCTTCTCTTCTTGGGTTGGGAAGAGAAACAGGAAAAAGGGTTGTCAATTTGCTATGGCATAGTCTTCATACATGGAAGTGGAGAATATCGGAAATGGAGTCCAAGCATGGTGATATCATTAGCAACTCTACCCCTGTGATCCAACAGGAAATTGAGCCAAATGGTGATGAAATCGGAAGGAACTCTATTCCAGTGATCCCACAGGAAATCCAGTCGGATAGTGATGATTTCAGAAGGGCCTTTATTTTCCCACAGAAGGTGGATACTAATGCTAATGAGTCCGATGATGTTAGCACTAACTCAATCCCAGTGATCCCACAGGAGGTGGAGTCTAATATTGTTGATGAGTCCAATGATGTTAGCACAAACTCAATCCCAGTGATCCCACAGGAGGTGGAGTCTAATATTGTTGATGAGTCCAATGATGTTAGCACAAACTCAATCCCAGTGATCCCACAGGAGGTGGAGTTTAATATTGTTGATGAGTCCAATGATGTTAGCACAAACCCAATCCCAGTGATCCCACAGGAGGTGGAGTCTAATATTGTTGATGAGTCCAATGATGTTAGCACAAACTCAATCCCAGTGATCCCACAGGAGGTGGAGTCTAATGTTGATAAGTCCAATGATGTTAGCACAAACTCATTCCCAGTGATCCCACAGGAAATGGAGCTTGAAGGTAATGATATAGGAAGGAACTCTATCCCCGTGACTCTGCAGGAAATAGAGCTTGATGGTGATGATATCAGAAGGAGCTCTATCGGGGAAATAGATCCTGCTGATATACAAATTAACTCTACAGTAGTCCTACAGAAAATTGAGTCCAATGGTGGTGATGTGAGAAGGAATTCCATTGCAGGTATCCCACAGGAAATAGAGTCCGAGGGTGGTGACATCAGAAGGAACTCTATCATAGTGATCCCGGTGGGAATAGAGGCCAATGGTGCTGATGTCAGAAGGAGGTCTATTGCTAGAAATATCCAATCCCGTTATCTCAGAGCTTCAATGGGATCTTGCCATGATTATTGCAAATATGGAATGAGGGAAGGATGTGAAGATGAGAATTTGGAAATATCTCCTATAGCTGGGAGGAAAAGGATATCCACAATCAACTCTAAGCCTTCTCCAGATTCTTTTGTCACAAAGAAAAGGGTCATATCAGTGACAAAGAAAGGAACTCCTTCTCCTAAAAAAGTTTCTAAGGAAGTTGGTGTTCCTAAGGAAGAGTCCATTCATTTGAGGAAAAAGCGTGGGCAATTTGAGCCGTCTTCTGAGGTGAAATCCCCAGTCAGTCATGAGCTTTCTCCGGATTCAGAAGCCCACATACTTAACAAGTCTTTTGTCACAGAGAAAAGGGTTGTGTCAGTGACAAAGAAAGGAACTGTTTCTTTGGAAAAAGAGATTGATGTTCCTATGGAAGATTCCATGGATTTGGGGGAGGAACCTGAGCAATTAGAGCCATCTTCTCTCCCAGGATCAGTCCGAAGCCTGAGAAATTTGAAAGTGATATCCACAATCAGTCCTAGGCCTTATCCAGGTTCTGCTGCTATGGAGAAGAAAGTTATATCAGTGAAAAAGAAAGATACTGCTTCTTCAAAAAATGAAATGCCTTCCTTAAAGGAAATTGATGCTTCTATGAAAGAGCAAGGTTCACGTCATAAAGGAAACATTGGAACCCGAAATAGCAAGGAAGCACCAGACAGTTCAAGCAGTGGAGGAAATACAAGTATCAGAAACCATAATAGGAGGGCTTCTCTACCGGGTGAGAAACAGACAATGGGGCTGCAACATGTTTCTTTGTCTTCCAAACGCTTCAATAAGAGAGACTCCAATGTGAATACCGGAAGCTCTTATAACCTTAAGGGGTTGACTCATCTGAAAGATCAAAATGATCCCGGAAAAGTTGAACCTCAGCCACCAAGTGATAAAGAAACGCCAGAAAAGATTTTGTATGTCATTGAATCAAGTACTGAGAATAGTACTATGAAATCAACTCCAAATGGTGTTACTGTTCCTGAGCCATCACCATCCTCAGCTGTATCTGTTGAAGGCAAAGGCAAAAGCTTGAAGCATGCAAGGAAGGGAACTGGTAGAACTGGATCCTCTCCATCCTCTGAGAAGAAAAACTTGAAACGCGTTAATGGAGCAGATTCTCGATTAGCTGGTCCTTTAGATCAGGATAACAATGGGTTGAGAAGAACTCGACGTGGTACTCAAGCATCTTTATCTCCATCGTCATCCTTATCATCCATGTCCATCTATTCATCTGACTCCACTCAACATAAAGAAAATGGTGCCAACTCTAAGCATGATAGAAGTAAAAATGTGAAGCCAATAGGAAAGTCGAAGGTGCAGCACAAGACTACTCCTCGGAGGGCTGCAATTGTTGGCTCTGGAAACAAAAACAGTGTATCCCGGAAGCTGAAATTTCAGAGAGGTAGGGTGATTGAACTTCAGCCTGAGAACAATACTCCAAGGAGACTCAAATTCAGGAGAGTAAGATTGGCATTGGAGGTTCAAAACAGTAAAGGCAGTATCAGAAGTGGAAGCATTGGGAGGAAGGAAGCTGATGACAGCCAGTCACCTGGTGCTGGTATCATCAAAAAGGGAAGCAATGTGAGGAAGGAAGTTGGTAACAGACAGTCAAATGGTGCTGGTATCAAAACGGTAAGCATTGTGAGGAAGGAAGTTGGTGATAGCCGGACAAGTGGTGTTGCTATCAAAAGGGGAAGCACTGGGAGGAAGGAAGTTTATGACAATCAGACAAATGGTGCTAAACTCAAATCTGAGAAAGTTGTTGTGAGAAACCTAGAACAAGTCGAAGTTTCCCCAAGGAAGAGCTTAACGAGGAAGGGGGTTGCCATTGGTCTTTTGAATGGCATCAGAAGCAATCCTGAGAAAGTGGTTTTGAGACACCAAGATGTAAAAGGAAAGAAAGGTGATCAGAAATTGTTCAACAATGTGATTGAAGAGACGGCCAGCAAGCTTGTTGAAACTAGGAAGAGTAAAGTCAAGGCCTTGGTTGGTGCTTTTGAGACTGTGATCTCTCTTCAGGACAACAGACCAGCGGCAACAGCAGATGCATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaM_binding

Protein Analysis

1058

Amino Acids

115.59

Weight (kDa)

9.3

Isoelectric Point (pI)

56.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CaM_binding PF07839 948 - 1044 2.6e-25 Plant calmodulin-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1938
Acc36I ACCTGC 1 cut(s) 932
AccB1I GGYRCC 1 cut(s) 2378
AccII CGCG 2 cut(s) 36, 2220
AccIII TCCGGA 1 cut(s) 1390
AciI CCGC 1 cut(s) 3152
AcoI YGGCCR 1 cut(s) 3060
AcsI RAATTY 5 cut(s) 931, 1147, 1579, 2510, 2570
AcuI CTGAAG 2 cut(s) 2522, 3119
AdeI CACNNNGTG 2 cut(s) 2006, 2672
AfaI GTAC 3 cut(s) 2053, 2065, 2296
AflII CTTAAG 1 cut(s) 1943
AflIII ACRYGT 2 cut(s) 1873, 2218
AhdI GACNNNNNGTC 1 cut(s) 2662
AjiI CACGTC 2 cut(s) 1739, 2291
AjnI CCWGG 3 cut(s) 1555, 1618, 2668
AloI GAACNNNNNNTCC 2 cut(s) 1252, 1284
Alw21I GWGCWC 1 cut(s) 842
Alw26I GTCTC 5 cut(s) 1902, 2557, 2989, 3050, 3113
AlwNI CAGNNNCTG 1 cut(s) 1625
Aor13HI TCCGGA 1 cut(s) 1390
AoxI GGCC 5 cut(s) 321, 1020, 1610, 3060, 3099
ApeKI GCWGC 4 cut(s) 1628, 1867, 2437, 2462
ApoI RAATTY 5 cut(s) 931, 1147, 1579, 2510, 2570
Asp700I GAANNNNTTC 4 cut(s) 931, 1264, 1468, 3035
AspA2I CCTAGG 1 cut(s) 1607
AspLEI GCGC 1 cut(s) 36
AspS9I GGNCC 2 cut(s) 321, 2249
AsuC2I CCSGG 4 cut(s) 1007, 1845, 1980, 2500
AvaII GGWCC 1 cut(s) 2249
AvrII CCTAGG 1 cut(s) 1607
AxyI CCTNAGG 1 cut(s) 1302
BaeGI GKGCMC 1 cut(s) 20
BamHI GGATCC 1 cut(s) 2183
BanI GGYRCC 1 cut(s) 2378
BanII GRGCYC 1 cut(s) 842
BarI GAAGNNNNNNTAC 2 cut(s) 1392, 1424
BbsI GAAGAC 3 cut(s) 140, 1344, 1878
Bbv12I GWGCWC 1 cut(s) 842
BbvCI CCTCAGC 2 cut(s) 1995, 2116
BbvI GCAGC 4 cut(s) 1615, 1854, 2449, 2449
BccI CCATC 7 cut(s) 812, 1552, 2113, 2119, 2200, 2323, 2348
BceAI ACGGC 3 cut(s) 26, 1333, 3075
BciT130I CCWGG 3 cut(s) 1557, 1620, 2670
BciVI GTATCC 3 cut(s) 340, 956, 2506
BclI TGATCA 1 cut(s) 3025
BcnI CCSGG 4 cut(s) 1007, 1845, 1980, 2500
BcoDI GTCTC 5 cut(s) 1902, 2557, 2989, 3050, 3113
BfaI CTAG 4 cut(s) 1056, 1608, 2894, 3081
BfmI CTRYAG 4 cut(s) 800, 882, 893, 1164
BfrI CTTAAG 1 cut(s) 1943
BfuAI ACCTGC 1 cut(s) 932
BfuI GTATCC 3 cut(s) 340, 956, 2506
BisI GCNGC 5 cut(s) 1629, 1868, 2438, 2463, 3153
BlnI CCTAGG 1 cut(s) 1607
BlsI GCNGC 5 cut(s) 1630, 1869, 2439, 2464, 3154
BmcAI AGTACT 2 cut(s) 2053, 2065
Bme1390I CCNGG 7 cut(s) 1007, 1557, 1620, 1845, 1980, 2500, 2670
Bme18I GGWCC 1 cut(s) 2249
BmeRI GACNNNNNGTC 1 cut(s) 2662
BmgBI CACGTC 2 cut(s) 1739, 2291
BmgT120I GGNCC 2 cut(s) 321, 2249
BmiI GGNNCC 4 cut(s) 1527, 1759, 2185, 2380
BmrFI CCNGG 7 cut(s) 1007, 1557, 1620, 1845, 1980, 2500, 2670
BmrI ACTGGG 8 cut(s) 386, 455, 524, 593, 662, 728, 1364, 2829
BmsI GCATC 4 cut(s) 1702, 2312, 2973, 3154
BmuI ACTGGG 8 cut(s) 386, 455, 524, 593, 662, 728, 1364, 2829
BpiI GAAGAC 3 cut(s) 140, 1344, 1878
BplI GAGNNNNNCTC 4 cut(s) 1822, 1854, 2269, 2301
BpmI CTGGAG 1 cut(s) 1194
Bpu10I CCTNAGC 4 cut(s) 1530, 1995, 2100, 2116
BpuEI CTTGAG 1 cut(s) 2283
BpuMI CCSGG 4 cut(s) 1007, 1845, 1980, 2500
BsaJI CCNNGG 8 cut(s) 966, 1509, 1555, 1607, 2453, 2558, 2915, 3102
BsaWI WCCGGW 2 cut(s) 1390, 1925
Bse21I CCTNAGG 1 cut(s) 1302
Bse3DI GCAATG 2 cut(s) 936, 2704
BseAI TCCGGA 1 cut(s) 1390
BseBI CCWGG 3 cut(s) 1557, 1620, 2670
BseDI CCNNGG 8 cut(s) 966, 1509, 1555, 1607, 2453, 2558, 2915, 3102
BseGI GGATG 5 cut(s) 1139, 2111, 2192, 2321, 2330
BseMI GCAATG 2 cut(s) 936, 2704
BseRI GAGGAG 1 cut(s) 2442
BseSI GKGCMC 1 cut(s) 20
BseXI GCAGC 4 cut(s) 1615, 1854, 2449, 2449
BseYI CCCAGC 1 cut(s) 1169
BsgI GTGCAG 1 cut(s) 2456
Bsh1236I CGCG 2 cut(s) 36, 2220
BshFI GGCC 5 cut(s) 323, 1022, 1612, 3062, 3101
BshNI GGYRCC 1 cut(s) 2378
BsiHKAI GWGCWC 1 cut(s) 842
BsiSI CCGG 7 cut(s) 1007, 1391, 1844, 1926, 1980, 2500, 2785
BsmAI GTCTC 5 cut(s) 1902, 2557, 2989, 3050, 3113
BsmBI CGTCTC 1 cut(s) 3050
BsnI GGCC 5 cut(s) 323, 1022, 1612, 3062, 3101
Bsp1286I GDGCHC 2 cut(s) 20, 842
Bsp13I TCCGGA 1 cut(s) 1390
Bsp19I CCATGG 1 cut(s) 1509
BspACI CCGC 1 cut(s) 3152
BspANI GGCC 5 cut(s) 323, 1022, 1612, 3062, 3101
BspEI TCCGGA 1 cut(s) 1390
BspFNI CGCG 2 cut(s) 36, 2220
BspHI TCATGA 1 cut(s) 1378
BspLI GGNNCC 4 cut(s) 1527, 1759, 2185, 2380
BspMAI CTGCAG 1 cut(s) 804
BspMI ACCTGC 1 cut(s) 932
BspQI GCTCTTC 1 cut(s) 2915
BspT107I GGYRCC 1 cut(s) 2378
BspTI CTTAAG 1 cut(s) 1943
BsrDI GCAATG 2 cut(s) 936, 2704
BssECI CCNNGG 8 cut(s) 966, 1509, 1555, 1607, 2453, 2558, 2915, 3102
BssT1I CCWWGG 5 cut(s) 1509, 1607, 2558, 2915, 3102
Bst2UI CCWGG 3 cut(s) 1557, 1620, 2670
Bst4CI ACNGT 9 cut(s) 886, 1468, 1787, 2095, 2492, 2609, 2727, 2752, 3124
Bst6I CTCTTC 7 cut(s) 96, 100, 1302, 2915, 3048, 3080, 3138
BstAFI CTTAAG 1 cut(s) 1943
BstC8I GCNNGC 3 cut(s) 2157, 3064, 3068
BstDSI CCRYGG 1 cut(s) 1509
BstENI CCTNNNNNAGG 1 cut(s) 1617
BstF5I GGATG 5 cut(s) 1139, 2111, 2192, 2321, 2330
BstFNI CGCG 2 cut(s) 36, 2220
BstHHI GCGC 1 cut(s) 36
BstMAI GTCTC 5 cut(s) 1902, 2557, 2989, 3050, 3113
BstMWI GCNNNNNNNGC 5 cut(s) 1028, 1345, 1540, 2970, 3158
BstNI CCWGG 3 cut(s) 1557, 1620, 2670
BstNSI RCATGY 3 cut(s) 1877, 2159, 3171
BstSCI CCNGG 7 cut(s) 1005, 1555, 1618, 1843, 1978, 2498, 2668
BstSFI CTRYAG 4 cut(s) 800, 882, 893, 1164
BstSLI GKGCMC 1 cut(s) 20
BstUI CGCG 2 cut(s) 36, 2220
BstV1I GCAGC 4 cut(s) 1615, 1854, 2449, 2449
BstV2I GAAGAC 3 cut(s) 140, 1344, 1878
BstX2I RGATCY 3 cut(s) 856, 1094, 2183
BstXI CCANNNNNNTGG 2 cut(s) 1516, 2669
BstYI RGATCY 3 cut(s) 856, 1094, 2183
Bsu36I CCTNAGG 1 cut(s) 1302
BsuI GTATCC 3 cut(s) 340, 956, 2506
BsuRI GGCC 5 cut(s) 323, 1022, 1612, 3062, 3101
BtgI CCRYGG 1 cut(s) 1509
BtrI CACGTC 2 cut(s) 1739, 2291
BtsCI GGATG 5 cut(s) 1139, 2111, 2192, 2321, 2330
BtsI GCAGTG 1 cut(s) 1801
BveI ACCTGC 1 cut(s) 932
Cac8I GCNNGC 3 cut(s) 2157, 3064, 3068
CaiI CAGNNNCTG 1 cut(s) 1625
CciI TCATGA 1 cut(s) 1378
CfoI GCGC 1 cut(s) 36
Cfr13I GGNCC 2 cut(s) 321, 2249
CsiI ACCWGGT 1 cut(s) 2668
Csp6I GTAC 3 cut(s) 2052, 2064, 2295
CviQI GTAC 3 cut(s) 2052, 2064, 2295
DraIII CACNNNGTG 2 cut(s) 2006, 2672
DriI GACNNNNNGTC 1 cut(s) 2662
EaeI YGGCCR 1 cut(s) 3060
Eam1104I CTCTTC 7 cut(s) 96, 100, 1302, 2915, 3048, 3080, 3138
Eam1105I GACNNNNNGTC 1 cut(s) 2662
EarI CTCTTC 7 cut(s) 96, 100, 1302, 2915, 3048, 3080, 3138
Ecl136II GAGCTC 1 cut(s) 840
Eco130I CCWWGG 5 cut(s) 1509, 1607, 2558, 2915, 3102
Eco147I AGGCCT 2 cut(s) 1612, 3101
Eco24I GRGCYC 1 cut(s) 842
Eco32I GATATC 4 cut(s) 199, 829, 1185, 1593
Eco47I GGWCC 1 cut(s) 2249
Eco53kI GAGCTC 1 cut(s) 840
Eco57I CTGAAG 2 cut(s) 2522, 3119
Eco81I CCTNAGG 1 cut(s) 1302
EcoICRI GAGCTC 1 cut(s) 840
EcoNI CCTNNNNNAGG 1 cut(s) 1617
EcoO109I RGGNCCY 1 cut(s) 321
EcoRI GAATTC 1 cut(s) 931
EcoRII CCWGG 3 cut(s) 1555, 1618, 2668
EcoRV GATATC 4 cut(s) 199, 829, 1185, 1593
EcoT14I CCWWGG 5 cut(s) 1509, 1607, 2558, 2915, 3102
EcoT22I ATGCAT 1 cut(s) 3169
EcoT38I GRGCYC 1 cut(s) 842
ErhI CCWWGG 5 cut(s) 1509, 1607, 2558, 2915, 3102
Esp3I CGTCTC 1 cut(s) 3050
FalI AAGNNNNNCTT 2 cut(s) 2195, 2227
FbaI TGATCA 1 cut(s) 3025
Fnu4HI GCNGC 5 cut(s) 1629, 1868, 2438, 2463, 3153
FokI GGATG 5 cut(s) 1146, 2098, 2179, 2308, 2317
FriOI GRGCYC 1 cut(s) 842
Fsp4HI GCNGC 5 cut(s) 1629, 1868, 2438, 2463, 3153
FspBI CTAG 4 cut(s) 1056, 1608, 2894, 3081
GlaI GCGC 1 cut(s) 35
GluI GCNGC 5 cut(s) 1629, 1868, 2438, 2463, 3153
GsaI CCCAGC 1 cut(s) 1173
GsuI CTGGAG 1 cut(s) 1194
HaeIII GGCC 5 cut(s) 323, 1022, 1612, 3062, 3101
HapII CCGG 7 cut(s) 1007, 1391, 1844, 1926, 1980, 2500, 2785
HhaI GCGC 1 cut(s) 36
Hin6I GCGC 1 cut(s) 34
HinP1I GCGC 1 cut(s) 34
HincII GTYRAC 1 cut(s) 1953
HindII GTYRAC 1 cut(s) 1953
HindIII AAGCTT 2 cut(s) 2146, 3068
HpaII CCGG 7 cut(s) 1007, 1391, 1844, 1926, 1980, 2500, 2785
Hpy166II GTNNAC 2 cut(s) 1736, 1953
Hpy8I GTNNAC 2 cut(s) 1736, 1953
Hpy99I CGWCG 1 cut(s) 2292
HpyCH4III ACNGT 9 cut(s) 886, 1468, 1787, 2095, 2492, 2609, 2727, 2752, 3124
HpyCH4IV ACGT 2 cut(s) 1738, 2290
HpyCH4V TGCA 8 cut(s) 802, 941, 1113, 1870, 2159, 2437, 2465, 3167
HpyF10VI GCNNNNNNNGC 5 cut(s) 1028, 1345, 1540, 2970, 3158
HpySE526I ACGT 2 cut(s) 1738, 2290
HspAI GCGC 1 cut(s) 34
Kpn2I TCCGGA 1 cut(s) 1390
Ksp22I TGATCA 1 cut(s) 3025
LguI GCTCTTC 1 cut(s) 2915
LmnI GCTCC 3 cut(s) 754, 837, 2228
Lsp1109I GCAGC 4 cut(s) 1615, 1854, 2449, 2449
LweI GCATC 4 cut(s) 1702, 2312, 2973, 3154
MabI ACCWGGT 1 cut(s) 2668
MaeI CTAG 4 cut(s) 1056, 1608, 2894, 3081
MaeII ACGT 2 cut(s) 1738, 2290
MaeIII GTNAC 9 cut(s) 793, 974, 1222, 1246, 1426, 1450, 2089, 2664, 2717
MfeI CAATTG 1 cut(s) 2466
MflI RGATCY 3 cut(s) 856, 1094, 2183
MhlI GDGCHC 2 cut(s) 20, 842
MluI ACGCGT 1 cut(s) 2218
MmeI TCCRAC 2 cut(s) 250, 279
Mph1103I ATGCAT 1 cut(s) 3169
MroI TCCGGA 1 cut(s) 1390
MroXI GAANNNNTTC 4 cut(s) 931, 1264, 1468, 3035
MseI TTAA 7 cut(s) 555, 876, 1413, 1700, 1944, 2223, 2927
MslI CAYNNNNRTG 3 cut(s) 160, 1244, 3003
MspA1I CMGCKG 2 cut(s) 2120, 3152
MspCI CTTAAG 1 cut(s) 1943
MspI CCGG 7 cut(s) 1007, 1391, 1844, 1926, 1980, 2500, 2785
MspR9I CCNGG 7 cut(s) 1007, 1557, 1620, 1845, 1980, 2500, 2670
MunI CAATTG 1 cut(s) 2466
MvaI CCWGG 3 cut(s) 1557, 1620, 2670
MvnI CGCG 2 cut(s) 36, 2220
MwoI GCNNNNNNNGC 5 cut(s) 1028, 1345, 1540, 2970, 3158
NciI CCSGG 4 cut(s) 1007, 1845, 1980, 2500
NcoI CCATGG 1 cut(s) 1509
NlaIV GGNNCC 4 cut(s) 1527, 1759, 2185, 2380
NmuCI GTSAC 7 cut(s) 793, 974, 1222, 1246, 1426, 1450, 2664
NsiI ATGCAT 1 cut(s) 3169
NspI RCATGY 3 cut(s) 1877, 2159, 3171
PaeI GCATGC 1 cut(s) 2159
PagI TCATGA 1 cut(s) 1378
PceI AGGCCT 2 cut(s) 1612, 3101
PciI ACATGT 1 cut(s) 1873
PciSI GCTCTTC 1 cut(s) 2915
PdmI GAANNNNTTC 4 cut(s) 931, 1264, 1468, 3035
PfeI GAWTC 6 cut(s) 50, 1214, 1394, 1505, 2045, 2234
PfoI TCCNGGA 2 cut(s) 1978, 2498
PkrI GCNGC 5 cut(s) 1630, 1869, 2439, 2464, 3154
PscI ACATGT 1 cut(s) 1873
PsiI TTATAA 1 cut(s) 1938
Psp124BI GAGCTC 1 cut(s) 842
Psp6I CCWGG 3 cut(s) 1555, 1618, 2668
PspFI CCCAGC 1 cut(s) 1169
PspGI CCWGG 3 cut(s) 1555, 1618, 2668
PspN4I GGNNCC 4 cut(s) 1527, 1759, 2185, 2380
PspPI GGNCC 2 cut(s) 321, 2249
PstI CTGCAG 1 cut(s) 804
PstNI CAGNNNCTG 1 cut(s) 1625
PsuI RGATCY 3 cut(s) 856, 1094, 2183
PvuII CAGCTG 1 cut(s) 2120
RsaI GTAC 3 cut(s) 2053, 2065, 2296
RsaNI GTAC 3 cut(s) 2052, 2064, 2295
RseI CAYNNNNRTG 3 cut(s) 160, 1244, 3003
SacI GAGCTC 1 cut(s) 842
SapI GCTCTTC 1 cut(s) 2915
SaqAI TTAA 7 cut(s) 555, 876, 1413, 1700, 1944, 2223, 2927
SatI GCNGC 5 cut(s) 1629, 1868, 2438, 2463, 3153
Sau96I GGNCC 2 cut(s) 321, 2249
ScaI AGTACT 2 cut(s) 2053, 2065
ScrFI CCNGG 7 cut(s) 1007, 1557, 1620, 1845, 1980, 2500, 2670
SduI GDGCHC 2 cut(s) 20, 842
SexAI ACCWGGT 1 cut(s) 2668
SfaNI GCATC 4 cut(s) 1702, 2312, 2973, 3154
SfcI CTRYAG 4 cut(s) 800, 882, 893, 1164
SinI GGWCC 1 cut(s) 2249
SmiMI CAYNNNNRTG 3 cut(s) 160, 1244, 3003
SmlI CTYRAG 2 cut(s) 1943, 2298
SmoI CTYRAG 2 cut(s) 1943, 2298
SphI GCATGC 1 cut(s) 2159
SseBI AGGCCT 2 cut(s) 1612, 3101
SsiI CCGC 1 cut(s) 3152
SspI AATATT 4 cut(s) 421, 490, 559, 628
SspMI CTAG 4 cut(s) 1056, 1608, 2894, 3081
SstI GAGCTC 1 cut(s) 842
StuI AGGCCT 2 cut(s) 1612, 3101
StyD4I CCNGG 7 cut(s) 1005, 1555, 1618, 1843, 1978, 2498, 2668
StyI CCWWGG 5 cut(s) 1509, 1607, 2558, 2915, 3102
TaaI ACNGT 9 cut(s) 886, 1468, 1787, 2095, 2492, 2609, 2727, 2752, 3124
TaiI ACGT 2 cut(s) 1741, 2293
TaqI TCGA 4 cut(s) 2239, 2287, 2429, 2904
TatI WGTACW 2 cut(s) 2051, 2063
TauI GCSGC 1 cut(s) 3155
TfiI GAWTC 6 cut(s) 50, 1214, 1394, 1505, 2045, 2234
Tru1I TTAA 7 cut(s) 555, 876, 1413, 1700, 1944, 2223, 2927
Tru9I TTAA 7 cut(s) 555, 876, 1413, 1700, 1944, 2223, 2927
TseFI GTSAC 7 cut(s) 793, 974, 1222, 1246, 1426, 1450, 2664
TseI GCWGC 4 cut(s) 1628, 1867, 2437, 2462
Tsp45I GTSAC 7 cut(s) 793, 974, 1222, 1246, 1426, 1450, 2664
TspDTI ATGAA 7 cut(s) 140, 267, 1307, 1701, 1736, 2084, 2337
Vha464I CTTAAG 1 cut(s) 1943
VpaK11BI GGWCC 1 cut(s) 2249
XagI CCTNNNNNAGG 1 cut(s) 1617
XapI RAATTY 5 cut(s) 931, 1147, 1579, 2510, 2570
XceI RCATGY 3 cut(s) 1877, 2159, 3171
XmaJI CCTAGG 1 cut(s) 1607
XmnI GAANNNNTTC 4 cut(s) 931, 1264, 1468, 3035
XspI CTAG 4 cut(s) 1056, 1608, 2894, 3081
ZrmI AGTACT 2 cut(s) 2053, 2065
Zsp2I ATGCAT 1 cut(s) 3169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.