pycom14g17160

Plant calmodulin-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0012620
Physical Location & Seq
Forward (+)
19226184 .. 19229474
3291 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g17160.1

Sequence Viewer

Length: 3291 bp
ATGGAAGTAGAGAACATCGGTATCCCAGTGATCCTACAAGTCCTGGACCTTCGTGGTGACAAGGCCAAAAATAATTCGATCCCACAAGAACCAGAGTCCAATTGGGGTGACGTTGGCAGTAATATCAGAAGGGATTCCATCCCTGAAATCCCACCGGAAATGGATCTCAAGGGTGGTGATGTCGGAGGTAACTTTGTCCTAGGGGTTCCACAGGACATAGAGCCCAATGGCAGTGATAGCAGAAGGAGCTCTATCGGAAATGTTGATCCTCCTCATATCAAAAGGAGCTCTATCCCAGTGATCCCACAGGAAATGGATCCCAAGGGTGGTGATGTTGGAGGTAATTCGGTCATAGGTAATTTGGTCGTAGGGATTCCACAGGACATAGAGCCCAATGGTGGTGACATCAGAAGGAGCTCTATTGGAAATGTTGATTCTCCTTCTACCGAAAGAAACTCTATCCCACTGGTCCCGCAGGACATGGATCTCAGCGATGATGATAGGAACTTTATTGCAACTGTCGCACGGGAAATAGAACCCGATGGCGGTGCTATCAAACAGAACTCTATCCTAGAGATCCCACAGAAAAAGGAGCCTGAGGGTGGAAACATCAGAAGCAAGTCTATCCTAGAGAACCTACAGGAAATGGAGTCTAATGCCGGTGATATCAGAAGAAGCTCCATTGATAAAGTAGATCCTCCTGCTGACATCAAAAGGATCTCTATCCCAGTGATCCCACAGGAAATGGAGCCTAATGGTGGTGATGGTAGAAGGAACTCTATCAGAGTGATCCCACAGGAATTAGAACCCAATGGCGGTGGTATCGGAAGGAACTCTATGCCAGCGGTCCCACAGAAAATGGAACCAAATGCTGGGGATACCAGCAGGAACTCTGTTCTGTGGATCCCACTTGAAATGGAACCAAAGGGTGGTAGAAGGATTCAGTCCCGATATCTCAGAGCTCCAACAGGTTCCTGCCATGATTATTGCAAATATGGAGTGAGAAATGCTTCTGAAGAAAATGCAACTGCCGCAATGCGAAAATCGGCAACAGAGAGGAAACAGATGTCCACTACAAATCAAAAGCCTTCTGATTCCGGCACCAAGAAGCTTGACAATTCTGCCATCACAGAGAAAAAAGTTTTGTCATTGAGGAAGAAGGAAATTGTTTCTGCAGAAAAGGTTCCATCCTCTTCCAAGGATATTGATGTTTCTATGGAAGATTCCTCCAGTTTGAAGGTGAAGCGTGTGCAATCAGAGCCATCTTCTCTCCCAGAACCGGCTTTAAGCCTGAGAAATTCAAAGGCGATATCCACAACTGGATCGAGGTCTGATCCACATTCCCAAACCTGGAAACTTAATAACTCTGTCGTCATGGAGAAAAAAGGTTCATCATCTACCAAGAAAGAAACCGCTTCTTCAAAGAAAGTTTCATCTCCCAAGGAAATAGCCATTTGTATGGAAGAGTTGATTGATTCGAAGATGAAAGCTGAGCAGTTAGATCAATCTTCTTTCCCAGGATCAGACCCAAGCCTGAGCAATTCGGAGGTGACATCGACAATCAATTCTAAGCCATCTCCAGATTCCGAGTCCCAGAATTCTATTGCCACGCAGAAAAGAGTTTCACCTTCCTACAAGGATATAGATGTTTCTGCTGAAGATTCCACCGATTCAAAAGTGATATCAACAGTCAAACCTAATCCTTCTCCAGATTCTAAAGCACGGAAACTTAATCATTCTGGTGTCATGGAGAAAAGAGTTTTATCATCAAAAAGGAAAGAAAATGTCTCTTCAAAAACAGTTTTAACTCCCATTAAGGAGATTGAAGTTTCTACAGAAGGTTCCATCGGTTCAAAGGTGAAGCGTGAGCAAGTAGAGTCATCTTCTTGCACAGGACAAGGTTCAAGTAATCAAGGAAAGAGTGGAACTAGAAAAGGCAAGGAAATACCTGAAGGTTCGAGCAGCAGTGTCAATAGAAAAAACAGAAGCAAACCGCCGAGGACTCCTGTATTGGTTGAGAAAAAGATGTTAGAGCCTGAAACTGTTTCTTTGACTTACAAACACCCCGTCAAGAGAGTCTCCAATGAAAATGCTGGAAGCTTTAAGAACCTTAAAGGGTTGTCTCATATGAAAGATCAAAATGGTCCCCGAAAAGTTGAACCTGAGGGACCCTGCAATAAGGATGTTCCAGAAAACATTTTGTATGTCATCGAGTCTAATACTGAGAATAGTACTGTCGAACCAACTCCAAATGGTGCCGATGCCCCTGAGTTATCATCGTCATCACATCAATCCTCGGAAGTCAAAAGCTCGAGACAAGCTAGGAAGGGAGTTGGTACCACTCGAGCACCTACATCCTCTATGAAGAAAAACTTGAAACGCACTGCAAATGGAGGTTATGGTCGTTCACCTACTGTTCCATCCCCTAAAAACAAAGGCTTGAGGTGTAGTACTCGAAGACCTTTATCTTCATCGTCATCCTTATCATCATCATCCGTGTCTGCATCCCCAGCCAAGTCCACTCATAAAAAAGAAAATGGCGCCACCTCTCAACGCAAAGCAATGAAAACTGGCAATCAAAGAGCAAATTTGAAGGTGGGGAAGAGTACTAGGCCTCGAAAGTCTGAGATACTTAGCTCTGAAAGTAAAAACAGCTCAGCCCGAAAGCTGACATTCAGGAGAGGAAGGGTGCTTGACATTAAGCCTGAGATCAATACTACAAGGAGACTCAAATTCAGGCGAGTAAGATTGGCAGGGGAGTCCCAAAATGGTAAGGGTGGTGTCATAGGGGGAAGCGCCGGGAGGAAAGAAGTATGCGACAGCCAGTCAATTGGTGATGTATTCCAAAGGTTAAGCCCCTGGTGGAAAGTAGTTGATGGCAGCCAGTCAAATGGTGCTGTTATCAAAAGGCGAAGCCTTGGGAGGATTCAAGTTGATGGCAGCCAGTCAAATGGTGGTAAATTCAAAAGCGAGAAAGTTGTTCCGAGAAGCTTCAAACGAGATGTGGAAGGTTCACAAAGGAAGAGCTTGAGGAGGAAAGCGGCCGGTGATAGTGGGTTGAATGGTAGTACTAAAACGAGTCCTGAGAAAGTTGTGTTGAGACACCAAGGTGTGACAGGAAGGAAGGATGTGCAGAAGTTGTTGAATAATGTGATTGAAGAGACTGCCAGTAGGCTTGTTGAGTCCAGGAAGAGTAAAGTCAAGGCCTTGGTTGGTGCTTTCGAGACTGTGATTTCGCTTCAGGATACCAAATCCATGGCATCGGAAACAGACACAGAAGATGCAGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaM_binding

Protein Analysis

1097

Amino Acids

118.52

Weight (kDa)

9.72

Isoelectric Point (pI)

65.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CaM_binding PF07839 989 - 1079 9.4e-27 Plant calmodulin-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 2336
AccB1I GGYRCC 4 cut(s) 1100, 2255, 2336, 2540
AccB7I CCANNNNNTGG 2 cut(s) 872, 929
AciI CCGC 8 cut(s) 473, 546, 816, 845, 1032, 1413, 1994, 3041
AcoI YGGCCR 1 cut(s) 3042
AcsI RAATTY 5 cut(s) 1297, 1597, 2587, 2732, 2960
AcuI CTGAAG 4 cut(s) 1035, 1677, 1971, 3224
AcyI GRCGYC 1 cut(s) 2541
AfaI GTAC 5 cut(s) 2233, 2338, 2452, 2608, 3070
AhdI GACNNNNNGTC 1 cut(s) 2824
AjnI CCWGG 5 cut(s) 42, 1349, 1516, 2858, 3185
AleI CACNNNNGTG 1 cut(s) 3108
Alw21I GWGCWC 5 cut(s) 251, 290, 419, 964, 2350
Alw26I GTCTC 8 cut(s) 1792, 2083, 2127, 2308, 2719, 3094, 3155, 3218
Ama87I CYCGRG 2 cut(s) 2311, 2343
AoxI GGCC 4 cut(s) 63, 2612, 3042, 3204
ApeKI GCWGC 3 cut(s) 1962, 2880, 2940
ApoI RAATTY 5 cut(s) 1297, 1597, 2587, 2732, 2960
ArsI GACNNNNNNTTYG 4 cut(s) 2052, 2084, 3217, 3249
Asp700I GAANNNNTTC 3 cut(s) 133, 1009, 1182
Asp718I GGTACC 1 cut(s) 2336
AspA2I CCTAGG 1 cut(s) 199
AspLEI GCGC 2 cut(s) 2543, 2798
AspS9I GGNCC 5 cut(s) 46, 469, 847, 2144, 2168
AsuC2I CCSGG 1 cut(s) 2800
AsuII TTCGAA 1 cut(s) 1478
AvaI CYCGRG 2 cut(s) 2311, 2343
AvaII GGWCC 5 cut(s) 46, 469, 847, 2144, 2168
AvrII CCTAGG 1 cut(s) 199
AxyI CCTNAGG 2 cut(s) 597, 2163
BaeI ACNNNNGTAYC 1 cut(s) 37
BamHI GGATCC 2 cut(s) 316, 903
BanI GGYRCC 4 cut(s) 1100, 2255, 2336, 2540
BanII GRGCYC 6 cut(s) 225, 251, 290, 393, 419, 964
BbsI GAAGAC 1 cut(s) 2464
Bbv12I GWGCWC 5 cut(s) 251, 290, 419, 964, 2350
BbvI GCAGC 3 cut(s) 1974, 2892, 2952
BcgI CGANNNNNNTGC 2 cut(s) 530, 564
BciT130I CCWGG 5 cut(s) 44, 1351, 1518, 2860, 3187
BciVI GTATCC 3 cut(s) 32, 871, 3238
BcnI CCSGG 1 cut(s) 2800
BcoDI GTCTC 8 cut(s) 1792, 2083, 2127, 2308, 2719, 3094, 3155, 3218
BfaI CTAG 6 cut(s) 200, 572, 629, 1929, 2322, 2610
BfmI CTRYAG 3 cut(s) 638, 1173, 1833
BfoI RGCGCY 2 cut(s) 2544, 2799
BfuI GTATCC 3 cut(s) 32, 871, 3238
BisI GCNGC 5 cut(s) 1032, 1963, 2881, 2941, 3042
BlnI CCTAGG 1 cut(s) 199
BlpI GCTNAGC 2 cut(s) 1491, 2656
BlsI GCNGC 5 cut(s) 1033, 1964, 2882, 2942, 3043
BmcAI AGTACT 4 cut(s) 2233, 2452, 2608, 3070
Bme1390I CCNGG 6 cut(s) 44, 1351, 1518, 2800, 2860, 3187
Bme18I GGWCC 5 cut(s) 46, 469, 847, 2144, 2168
BmeRI GACNNNNNGTC 1 cut(s) 2824
BmeT110I CYCGRG 2 cut(s) 2311, 2343
BmgT120I GGNCC 5 cut(s) 46, 469, 847, 2144, 2168
BmrFI CCNGG 6 cut(s) 44, 1351, 1518, 2800, 2860, 3187
BmrI ACTGGG 3 cut(s) 20, 290, 722
BmsI GCATC 4 cut(s) 2251, 2513, 3269, 3271
BmuI ACTGGG 3 cut(s) 20, 290, 722
BpiI GAAGAC 1 cut(s) 2464
BpmI CTGGAG 3 cut(s) 1213, 1563, 1692
Bpu10I CCTNAGC 1 cut(s) 1535
Bpu1102I GCTNAGC 2 cut(s) 1491, 2656
Bpu14I TTCGAA 1 cut(s) 1478
BpuEI CTTGAG 3 cut(s) 152, 2461, 3049
BpuMI CCSGG 1 cut(s) 2800
BsaBI GATNNNNATC 1 cut(s) 722
BsaHI GRCGYC 1 cut(s) 2541
BsaWI WCCGGW 1 cut(s) 154
Bse118I RCCGGY 3 cut(s) 659, 1279, 3044
Bse21I CCTNAGG 2 cut(s) 597, 2163
Bse3DI GCAATG 2 cut(s) 1041, 2568
Bse8I GATNNNNATC 1 cut(s) 722
BseBI CCWGG 5 cut(s) 44, 1351, 1518, 2860, 3187
BseGI GGATG 9 cut(s) 138, 1187, 2188, 2354, 2420, 2477, 2492, 2504, 3133
BseJI GATNNNNATC 1 cut(s) 722
BseMI GCAATG 2 cut(s) 1041, 2568
BseRI GAGGAG 2 cut(s) 261, 3046
BseX3I CGGCCG 1 cut(s) 3042
BseXI GCAGC 3 cut(s) 1974, 2892, 2952
BseYI CCCAGC 2 cut(s) 872, 2509
BsgI GTGCAG 1 cut(s) 3152
Bsh1285I CGRYCG 1 cut(s) 3045
BshFI GGCC 4 cut(s) 65, 2614, 3044, 3206
BshNI GGYRCC 4 cut(s) 1100, 2255, 2336, 2540
BsiEI CGRYCG 1 cut(s) 3045
BsiHKAI GWGCWC 5 cut(s) 251, 290, 419, 964, 2350
BsiHKCI CYCGRG 2 cut(s) 2311, 2343
BsiSI CCGG 6 cut(s) 155, 660, 1098, 1280, 2799, 3045
BslFI GGGAC 7 cut(s) 455, 833, 931, 1576, 2130, 2181, 2746
BsmAI GTCTC 8 cut(s) 1792, 2083, 2127, 2308, 2719, 3094, 3155, 3218
BsmFI GGGAC 7 cut(s) 455, 833, 931, 1576, 2130, 2181, 2746
BsnI GGCC 4 cut(s) 65, 2614, 3044, 3206
BsoBI CYCGRG 2 cut(s) 2311, 2343
Bsp119I TTCGAA 1 cut(s) 1478
Bsp1286I GDGCHC 7 cut(s) 225, 251, 290, 393, 419, 964, 2350
Bsp1720I GCTNAGC 2 cut(s) 1491, 2656
Bsp19I CCATGG 1 cut(s) 3255
BspACI CCGC 8 cut(s) 473, 546, 816, 845, 1032, 1413, 1994, 3041
BspANI GGCC 4 cut(s) 65, 2614, 3044, 3206
BspMAI CTGCAG 1 cut(s) 1177
BspQI GCTCTTC 1 cut(s) 3017
BspT104I TTCGAA 1 cut(s) 1478
BspT107I GGYRCC 4 cut(s) 1100, 2255, 2336, 2540
BsrDI GCAATG 2 cut(s) 1041, 2568
BsrFI RCCGGY 3 cut(s) 659, 1279, 3044
BssAI RCCGGY 3 cut(s) 659, 1279, 3044
BssNI GRCGYC 1 cut(s) 2541
BssT1I CCWWGG 8 cut(s) 199, 321, 1197, 1440, 2917, 3106, 3207, 3255
Bst2UI CCWGG 5 cut(s) 44, 1351, 1518, 2860, 3187
Bst4CI ACNGT 7 cut(s) 520, 1690, 1801, 2044, 2236, 2416, 3229
Bst6I CTCTTC 7 cut(s) 1198, 1458, 1795, 2597, 3017, 3153, 3185
BstACI GRCGYC 1 cut(s) 2541
BstBI TTCGAA 1 cut(s) 1478
BstC8I GCNNGC 1 cut(s) 843
BstDSI CCRYGG 1 cut(s) 3255
BstF5I GGATG 9 cut(s) 138, 1187, 2188, 2354, 2420, 2477, 2492, 2504, 3133
BstH2I RGCGCY 2 cut(s) 2544, 2799
BstHHI GCGC 2 cut(s) 2543, 2798
BstMAI GTCTC 8 cut(s) 1792, 2083, 2127, 2308, 2719, 3094, 3155, 3218
BstMCI CGRYCG 1 cut(s) 3045
BstMWI GCNNNNNNNGC 6 cut(s) 237, 246, 521, 1031, 1258, 2510
BstNI CCWGG 5 cut(s) 44, 1351, 1518, 2860, 3187
BstSCI CCNGG 6 cut(s) 42, 1349, 1516, 2798, 2858, 3185
BstSFI CTRYAG 3 cut(s) 638, 1173, 1833
BstV1I GCAGC 3 cut(s) 1974, 2892, 2952
BstV2I GAAGAC 1 cut(s) 2464
BstX2I RGATCY 7 cut(s) 163, 316, 484, 576, 694, 717, 903
BstXI CCANNNNNNTGG 5 cut(s) 1459, 2831, 2891, 2951, 3256
BstYI RGATCY 7 cut(s) 163, 316, 484, 576, 694, 717, 903
BstZI CGGCCG 1 cut(s) 3042
Bsu36I CCTNAGG 2 cut(s) 597, 2163
BsuI GTATCC 3 cut(s) 32, 871, 3238
BsuRI GGCC 4 cut(s) 65, 2614, 3044, 3206
BtgI CCRYGG 1 cut(s) 3255
BtgZI GCGATG 1 cut(s) 507
BtsCI GGATG 9 cut(s) 138, 1187, 2188, 2354, 2420, 2477, 2492, 2504, 3133
BtsI GCAGTG 3 cut(s) 238, 1972, 2382
BtsIMutI CAGTG 7 cut(s) 33, 238, 303, 464, 735, 1972, 2382
Cac8I GCNNGC 1 cut(s) 843
CfoI GCGC 2 cut(s) 2543, 2798
Cfr10I RCCGGY 3 cut(s) 659, 1279, 3044
Cfr13I GGNCC 5 cut(s) 46, 469, 847, 2144, 2168
Csp6I GTAC 5 cut(s) 2232, 2337, 2451, 2607, 3069
CspCI CAANNNNNGTGG 2 cut(s) 799, 834
CviAII CATG 5 cut(s) 481, 980, 1375, 1747, 3256
CviQI GTAC 5 cut(s) 2232, 2337, 2451, 2607, 3069
DinI GGCGCC 1 cut(s) 2542
DriI GACNNNNNGTC 1 cut(s) 2824
EaeI YGGCCR 1 cut(s) 3042
EagI CGGCCG 1 cut(s) 3042
Eam1104I CTCTTC 7 cut(s) 1198, 1458, 1795, 2597, 3017, 3153, 3185
Eam1105I GACNNNNNGTC 1 cut(s) 2824
EarI CTCTTC 7 cut(s) 1198, 1458, 1795, 2597, 3017, 3153, 3185
Ecl136II GAGCTC 4 cut(s) 249, 288, 417, 962
EclXI CGGCCG 1 cut(s) 3042
Eco130I CCWWGG 8 cut(s) 199, 321, 1197, 1440, 2917, 3106, 3207, 3255
Eco147I AGGCCT 2 cut(s) 2614, 3206
Eco24I GRGCYC 6 cut(s) 225, 251, 290, 393, 419, 964
Eco32I GATATC 4 cut(s) 667, 953, 1311, 1683
Eco47I GGWCC 5 cut(s) 46, 469, 847, 2144, 2168
Eco52I CGGCCG 1 cut(s) 3042
Eco53kI GAGCTC 4 cut(s) 249, 288, 417, 962
Eco57I CTGAAG 4 cut(s) 1035, 1677, 1971, 3224
Eco81I CCTNAGG 2 cut(s) 597, 2163
Eco88I CYCGRG 2 cut(s) 2311, 2343
EcoICRI GAGCTC 4 cut(s) 249, 288, 417, 962
EcoO109I RGGNCCY 1 cut(s) 2168
EcoRI GAATTC 1 cut(s) 1597
EcoRII CCWGG 5 cut(s) 42, 1349, 1516, 2858, 3185
EcoRV GATATC 4 cut(s) 667, 953, 1311, 1683
EcoT14I CCWWGG 8 cut(s) 199, 321, 1197, 1440, 2917, 3106, 3207, 3255
EcoT38I GRGCYC 6 cut(s) 225, 251, 290, 393, 419, 964
EgeI GGCGCC 1 cut(s) 2542
EheI GGCGCC 1 cut(s) 2542
ErhI CCWWGG 8 cut(s) 199, 321, 1197, 1440, 2917, 3106, 3207, 3255
FaeI CATG 5 cut(s) 484, 983, 1378, 1750, 3259
FalI AAGNNNNNCTT 2 cut(s) 2357, 2389
FaqI GGGAC 7 cut(s) 455, 833, 931, 1576, 2130, 2181, 2746
FatI CATG 5 cut(s) 480, 979, 1374, 1746, 3255
FauI CCCGC 1 cut(s) 480
FauNDI CATATG 1 cut(s) 2127
Fnu4HI GCNGC 5 cut(s) 1032, 1963, 2881, 2941, 3042
FokI GGATG 9 cut(s) 125, 1174, 2195, 2341, 2407, 2464, 2479, 2491, 3140
FriOI GRGCYC 6 cut(s) 225, 251, 290, 393, 419, 964
Fsp4HI GCNGC 5 cut(s) 1032, 1963, 2881, 2941, 3042
FspBI CTAG 6 cut(s) 200, 572, 629, 1929, 2322, 2610
GlaI GCGC 2 cut(s) 2542, 2797
GluI GCNGC 5 cut(s) 1032, 1963, 2881, 2941, 3042
GsaI CCCAGC 2 cut(s) 876, 2513
GsuI CTGGAG 3 cut(s) 1213, 1563, 1692
HaeII RGCGCY 2 cut(s) 2544, 2799
HaeIII GGCC 4 cut(s) 65, 2614, 3044, 3206
HapII CCGG 6 cut(s) 155, 660, 1098, 1280, 2799, 3045
HhaI GCGC 2 cut(s) 2543, 2798
Hin1I GRCGYC 1 cut(s) 2541
Hin1II CATG 5 cut(s) 484, 983, 1378, 1750, 3259
Hin6I GCGC 2 cut(s) 2541, 2796
HinP1I GCGC 2 cut(s) 2541, 2796
HindIII AAGCTT 3 cut(s) 1109, 2098, 2989
HpaII CCGG 6 cut(s) 155, 660, 1098, 1280, 2799, 3045
Hpy166II GTNNAC 4 cut(s) 1071, 2408, 2520, 3014
Hpy8I GTNNAC 4 cut(s) 1071, 2408, 2520, 3014
HpyCH4III ACNGT 7 cut(s) 520, 1690, 1801, 2044, 2236, 2416, 3229
HpyCH4IV ACGT 1 cut(s) 111
HpyF10VI GCNNNNNNNGC 6 cut(s) 237, 246, 521, 1031, 1258, 2510
HpySE526I ACGT 1 cut(s) 111
Hsp92I GRCGYC 1 cut(s) 2541
Hsp92II CATG 5 cut(s) 484, 983, 1378, 1750, 3259
HspAI GCGC 2 cut(s) 2541, 2796
KasI GGCGCC 1 cut(s) 2540
KflI GGGWCCC 1 cut(s) 2168
KpnI GGTACC 1 cut(s) 2340
LguI GCTCTTC 1 cut(s) 3017
LmnI GCTCC 7 cut(s) 246, 285, 414, 592, 683, 748, 967
Lsp1109I GCAGC 3 cut(s) 1974, 2892, 2952
LweI GCATC 4 cut(s) 2251, 2513, 3269, 3271
MaeI CTAG 6 cut(s) 200, 572, 629, 1929, 2322, 2610
MaeII ACGT 1 cut(s) 111
MaeIII GTNAC 6 cut(s) 56, 107, 188, 401, 1549, 3112
MfeI CAATTG 2 cut(s) 100, 2829
MflI RGATCY 7 cut(s) 163, 316, 484, 576, 694, 717, 903
MhlI GDGCHC 7 cut(s) 225, 251, 290, 393, 419, 964, 2350
Mly113I GGCGCC 1 cut(s) 2541
MmeI TCCRAC 3 cut(s) 163, 316, 989
MroXI GAANNNNTTC 3 cut(s) 133, 1009, 1182
MseI TTAA 9 cut(s) 1286, 1359, 1731, 1805, 1815, 2103, 2112, 2700, 2852
MslI CAYNNNNRTG 3 cut(s) 1457, 1740, 3108
MspA1I CMGCKG 1 cut(s) 845
MspI CCGG 6 cut(s) 155, 660, 1098, 1280, 2799, 3045
MspR9I CCNGG 6 cut(s) 44, 1351, 1518, 2800, 2860, 3187
MunI CAATTG 2 cut(s) 100, 2829
MvaI CCWGG 5 cut(s) 44, 1351, 1518, 2860, 3187
MwoI GCNNNNNNNGC 6 cut(s) 237, 246, 521, 1031, 1258, 2510
NarI GGCGCC 1 cut(s) 2541
NciI CCSGG 1 cut(s) 2800
NcoI CCATGG 1 cut(s) 3255
NdeI CATATG 1 cut(s) 2127
NlaIII CATG 5 cut(s) 484, 983, 1378, 1750, 3259
NmeAIII GCCGAG 1 cut(s) 2022
NmuCI GTSAC 5 cut(s) 56, 107, 401, 1549, 3112
NspV TTCGAA 1 cut(s) 1478
OliI CACNNNNGTG 1 cut(s) 3108
PaeR7I CTCGAG 2 cut(s) 2311, 2343
PceI AGGCCT 2 cut(s) 2614, 3206
PciSI GCTCTTC 1 cut(s) 3017
PdmI GAANNNNTTC 3 cut(s) 133, 1009, 1182
PflMI CCANNNNNTGG 2 cut(s) 872, 929
PfoI TCCNGGA 2 cut(s) 42, 3185
PkrI GCNGC 5 cut(s) 1033, 1964, 2882, 2942, 3043
PluTI GGCGCC 1 cut(s) 2544
PpuMI RGGWCCY 1 cut(s) 2168
Psp124BI GAGCTC 4 cut(s) 251, 290, 419, 964
Psp5II RGGWCCY 1 cut(s) 2168
Psp6I CCWGG 5 cut(s) 42, 1349, 1516, 2858, 3185
PspFI CCCAGC 2 cut(s) 872, 2509
PspGI CCWGG 5 cut(s) 42, 1349, 1516, 2858, 3185
PspPI GGNCC 5 cut(s) 46, 469, 847, 2144, 2168
PspPPI RGGWCCY 1 cut(s) 2168
PspXI VCTCGAGB 1 cut(s) 2343
PstI CTGCAG 1 cut(s) 1177
PsuI RGATCY 7 cut(s) 163, 316, 484, 576, 694, 717, 903
RsaI GTAC 5 cut(s) 2233, 2338, 2452, 2608, 3070
RsaNI GTAC 5 cut(s) 2232, 2337, 2451, 2607, 3069
RseI CAYNNNNRTG 3 cut(s) 1457, 1740, 3108
SacI GAGCTC 4 cut(s) 251, 290, 419, 964
SapI GCTCTTC 1 cut(s) 3017
SaqAI TTAA 9 cut(s) 1286, 1359, 1731, 1805, 1815, 2103, 2112, 2700, 2852
SatI GCNGC 5 cut(s) 1032, 1963, 2881, 2941, 3042
Sau96I GGNCC 5 cut(s) 46, 469, 847, 2144, 2168
ScaI AGTACT 4 cut(s) 2233, 2452, 2608, 3070
ScrFI CCNGG 6 cut(s) 44, 1351, 1518, 2800, 2860, 3187
SduI GDGCHC 7 cut(s) 225, 251, 290, 393, 419, 964, 2350
SfaNI GCATC 4 cut(s) 2251, 2513, 3269, 3271
SfcI CTRYAG 3 cut(s) 638, 1173, 1833
SfoI GGCGCC 1 cut(s) 2542
Sfr274I CTCGAG 2 cut(s) 2311, 2343
SfuI TTCGAA 1 cut(s) 1478
SinI GGWCC 5 cut(s) 46, 469, 847, 2144, 2168
SlaI CTCGAG 2 cut(s) 2311, 2343
SmiMI CAYNNNNRTG 3 cut(s) 1457, 1740, 3108
SmlI CTYRAG 5 cut(s) 167, 2311, 2343, 2440, 3028
SmoI CTYRAG 5 cut(s) 167, 2311, 2343, 2440, 3028
SseBI AGGCCT 2 cut(s) 2614, 3206
SsiI CCGC 8 cut(s) 473, 546, 816, 845, 1032, 1413, 1994, 3041
SspDI GGCGCC 1 cut(s) 2540
SspMI CTAG 6 cut(s) 200, 572, 629, 1929, 2322, 2610
SstI GAGCTC 4 cut(s) 251, 290, 419, 964
StuI AGGCCT 2 cut(s) 2614, 3206
StyD4I CCNGG 6 cut(s) 42, 1349, 1516, 2798, 2858, 3185
StyI CCWWGG 8 cut(s) 199, 321, 1197, 1440, 2917, 3106, 3207, 3255
TaaI ACNGT 7 cut(s) 520, 1690, 1801, 2044, 2236, 2416, 3229
TaiI ACGT 1 cut(s) 114
TaqII GACCGA 1 cut(s) 337
TatI WGTACW 4 cut(s) 2231, 2450, 2606, 3068
TauI GCSGC 2 cut(s) 1034, 3044
Tru1I TTAA 9 cut(s) 1286, 1359, 1731, 1805, 1815, 2103, 2112, 2700, 2852
Tru9I TTAA 9 cut(s) 1286, 1359, 1731, 1805, 1815, 2103, 2112, 2700, 2852
TscAI CASTG 7 cut(s) 33, 238, 303, 471, 735, 1972, 2389
TseFI GTSAC 5 cut(s) 56, 107, 401, 1549, 3112
TseI GCWGC 3 cut(s) 1962, 2880, 2940
Tsp45I GTSAC 5 cut(s) 56, 107, 401, 1549, 3112
TspDTI ATGAA 8 cut(s) 1380, 1422, 1499, 2100, 2144, 2378, 2460, 2579
TspGWI ACGGA 2 cut(s) 1738, 2485
TspRI CASTG 7 cut(s) 33, 238, 303, 471, 735, 1972, 2389
Van91I CCANNNNNTGG 2 cut(s) 872, 929
VpaK11BI GGWCC 5 cut(s) 46, 469, 847, 2144, 2168
XapI RAATTY 5 cut(s) 1297, 1597, 2587, 2732, 2960
XcmI CCANNNNNNNNNTGG 2 cut(s) 99, 2951
XhoI CTCGAG 2 cut(s) 2311, 2343
XmaJI CCTAGG 1 cut(s) 199
XmnI GAANNNNTTC 3 cut(s) 133, 1009, 1182
XspI CTAG 6 cut(s) 200, 572, 629, 1929, 2322, 2610
ZrmI AGTACT 4 cut(s) 2233, 2452, 2608, 3070
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.