Rh7BG050100

Plant calmodulin-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0012620
Physical Location & Seq
Forward (+)
3291878 .. 3296624
4747 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG050100.1

Sequence Viewer

Length: 2958 bp
ATGGAAGTAGAGAATATCGGTATCCCAGTGATCCTACAAGACTTGAAGTTTATTTGTGACGATGGCAGAAGGAACTCTATCTCAGTGATCCCACAAGAAAAAGAACCCAATGGCAGTCATATTAGAAGGCATTCTATAGCACTGGTTCCACAGGAAATGGAGTCCAAGCGTGGTGATATCATTAGCAACTCTATCCCAGTGATCCAACAGGAAATTGAGCCAAATGGTGATGAAATCGGGAGGAACTCTATTCCAGTGATCCCACAGGAAATCGAGTCGGATAGTGATGATTTCAGAAGGGCCTTTATTTTCCCACAGAAGGTGGAGTCGAATGCTGATGAGTCTGATGATATTAGCACTACATCAATCCCAGTGACCCCACAGGAGGTGGAGTCTATTGTTGATGAGTCCAATGATGTTAGCACAAACTCAATCCCTGTGATCCCACAGGAAACGGAGCTCGAAGATAGTGATATAGGAAGGAACTCTATCCCCGAGACTCCGCAGGAAATAGAGCTTGATGGTGATGATATCAAAAGGAGCTCTATCGGGGAAATAGATCCTGCTGATATACAAACCAACTCTATTCCAGTGATCCTACAGGAAATTGAGTCCAATGGTAGTGATGTGAGAAGGAACTCTATTGCAGTTATCCCACAGGAAATGGAGCCCGAGGGTGGTGACATCAGAAGGAACTCTGTCCTAGTGATCCCGGTGGGAATAGAGGCCAATGGTGCGGATGTCAGAAGGAGGTCTATTGCTAGAAATATCCAATCCCGTTATCTCAGAGCTTCAATGGGATCTTGCCATGATTATTGCAAATATGGAATGAGGGAAGGATGTGAAGATGAGAACTTGGAAATATCTCCTATAGCTGGGAGGAAAATGATATCCACAATCAACTCTAAGCCTTCTCCAGATTCTCTTGTCACAAAGAAAAGGGTCATATCAGTGACAAGGAAAGGAACTCCTTCTCCTAAAAAAGTTTCTAAGGAAGTTGATGTTCCTAAGGAAGAGTCCATTCATTTGAGGAAAAAGCGTGGGCAATTTGAGCCATCTTCTAAGGTGAAATCCCCAGTCAGTCATGAGCCCTCTCCGGATTCAGAAGCCCACATACTTAACAAGTCTTTTGTCATAGAGAAAAGGGTTGTATCAGTGACAAAGAAAGGAACTGTTTCTTTGGAAAAAGAAATTGATGTTACTATGGAAGATTCCATGGATTTGGAGGAGGAACCTGAGCAATTAGAGTCATCTTCTCTCCCAGGATCAGTCCGAAGCCTGAGAAACTCGAAAGTGATATCTACAATCAGTCCTAGGCCTTATCCAGGTTCTGCTGCTATGGAGAAGAAAGTTATATCAGTGAAAAAGAAAGATACTGCTTCTTCAAAAAATGAAATGCCTTCCATTAAGGAAATTGATGTTTCTATGGAAGAGCAAGGTTCACGTCATAAAGGAAACATTGGAACCAGGAATAGCAAGGAAGCACCAGACGGTTCAAGCAGTGGAGGAAATAGAAGTATCAGAAACCATAATAGAAGGGCTTTTCTACCGGGTGAGAAACGGACAATGGGGCTGCAAACTGTTTCGTTGTCTTCCAAACAGTTCAATAAGAGAGACTCCACCGTGAATACCGGAAGCTCTTATAACCTTAAGGGGTTGTCTCATCTGAATGATCAAAATGATCCCGGAAATGTTGAACCTCAGCCACCAAGTGATAAGGATACGCCAGAAAAGATTTTGTATGTCATTGAATCAAGTACTGAGAATAGTACTATGGAATCAACTCCAAATGGTGCTAGTGTTCCTGAGCCATCACCATCCTCAGCTGTATCTGTTGAAGGCAAAGGCAAAAGCTTGAAGCATGCAAGGAAGGGAATTGGTAGAACTGGATCCTCTCCATCCTCTGAGAAGAAAAGCTTGAAACGTGTTAATGGAGCTGATTCTCGATCAGCTGGTCCTTTAGATCAGGATAACAAGGGGTTGAGACGAACTCGACGTGGTACTCAAGCATCTTTATCTCCATCGTCATCCTCATCATCCATGTCCATCTATTCATCTGAGTCCACTCAACATAGAGAAAATGGTGCCAACTCTCAGCATGATAGAAGTAAAAATGTGAAGCCAATAGGAAAGTCGAAGGTGCAGCACAAGACTACTCCTCGGAGGGCTGCAATTGTTGGCTCTGGAAACAAAAACAGTGTATCCCGGAAGCTGACATTTCAGAGAGGTAGGGTGATTGAACTTCAGCCTGAGAACAATACTCCAAGGAGACTCAAATTCAGGCGAGTAAGATCGACGGTGGAGGTTCAAAACAGTAAAGGAAGTCTTCAGCCTGCGAAGAATACTCCAAGGAGACTCAAATTCAGGCGAGTAAGATCGACGGTTCAAAACAGTAAAGGAAGTATCACAAGTGGAAGCATTGGGAGGAAGGAAGTTGATGACAGCCAGTCAACTGGTGCTGGTATCATCAAAAAGGGAAGCAATGTGAGGAAGGAAGTTGGTAACAGACGGTCAAATGGTGCTGGTATCAAAACGGTAAGCATTGTGAGGAAGGAAGTTGGTGATAGCCAGTCAAGTGGTGTTGCTATCAAAAGGGGAATCATTGGGAGGAAGGAAGTTGATGACGATCAGACAAATGGTGCTAAACTCAAATCCGAGAAATTTGTTCTGAGAAACCTAGAACAAGTTAAAGTTTCCCCAAGGAAGAGCTTAACGAGGAAGGGGGTTGCTGTTGGTCTTTTGAATGGCATCAGAAGCAATCCTGAGAAAGTGGTTTTGAGACACCAAGATGTAAAAGGAAAGAAAGGCAATCAGAAATTGTTTAACAATGTGATTGAAGAGACGGCCAGCAAGCTTGTCGAAACTAGGAAGAGTAAAGTCAAGGCCTTGGTTGGTGCTTTTGAGACTGTGATCTCTCTTCAGGACACCAGACCAGCGGCAACGGCAGATGCATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaM_binding

Protein Analysis

985

Amino Acids

107.73

Weight (kDa)

9.56

Isoelectric Point (pI)

64.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CaM_binding PF07839 876 - 972 2.4e-26 Plant calmodulin-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1644
AccB1I GGYRCC 1 cut(s) 2084
AccIII TCCGGA 1 cut(s) 1096
AciI CCGC 3 cut(s) 503, 737, 2936
AcoI YGGCCR 1 cut(s) 2844
AcsI RAATTY 3 cut(s) 2276, 2360, 2660
AcuI CTGAAG 3 cut(s) 2228, 2312, 2903
AdeI CACNNNGTG 1 cut(s) 1712
AfaI GTAC 3 cut(s) 1759, 1771, 2002
AfiI CCNNNNNNNGG 5 cut(s) 319, 385, 677, 875, 1325
AflII CTTAAG 1 cut(s) 1649
AflIII ACRYGT 1 cut(s) 1924
AhdI GACNNNNNGTC 1 cut(s) 2446
AjiI CACGTC 2 cut(s) 1445, 1997
AjnI CCWGG 3 cut(s) 1261, 1324, 1466
AloI GAACNNNNNNTCC 2 cut(s) 958, 990
Alw21I GWGCWC 2 cut(s) 462, 545
Alw26I GTCTC 9 cut(s) 491, 1608, 1665, 1978, 2263, 2347, 2773, 2834, 2897
AlwNI CAGNNNCTG 1 cut(s) 1331
Ama87I CYCGRG 2 cut(s) 494, 671
Aor13HI TCCGGA 1 cut(s) 1096
AoxI GGCC 5 cut(s) 300, 726, 1316, 2844, 2883
ApeKI GCWGC 4 cut(s) 1334, 1573, 2143, 2168
ApoI RAATTY 3 cut(s) 2276, 2360, 2660
Asp700I GAANNNNTTC 3 cut(s) 130, 970, 1174
AspA2I CCTAGG 1 cut(s) 1313
AspS9I GGNCC 2 cut(s) 300, 1955
AsuC2I CCSGG 4 cut(s) 713, 1551, 1686, 2206
AsuHPI GGTGA 9 cut(s) 185, 239, 536, 692, 1078, 1565, 1806, 2245, 2573
AvaI CYCGRG 2 cut(s) 494, 671
AvaII GGWCC 1 cut(s) 1955
AvrII CCTAGG 1 cut(s) 1313
AxyI CCTNAGG 1 cut(s) 1008
BamHI GGATCC 1 cut(s) 1889
BanI GGYRCC 1 cut(s) 2084
BanII GRGCYC 4 cut(s) 462, 545, 672, 1092
BarI GAAGNNNNNNTAC 2 cut(s) 1098, 1130
BbsI GAAGAC 2 cut(s) 1584, 2318
Bbv12I GWGCWC 2 cut(s) 462, 545
BbvCI CCTCAGC 2 cut(s) 1701, 1822
BbvI GCAGC 4 cut(s) 1321, 1560, 2155, 2155
BccI CCATC 8 cut(s) 56, 515, 1063, 1819, 1825, 1906, 2029, 2054
BceAI ACGGC 1 cut(s) 2859
BcgI CGANNNNNNTGC 2 cut(s) 2839, 2873
BciT130I CCWGG 3 cut(s) 1263, 1326, 1468
BciVI GTATCC 3 cut(s) 32, 1714, 2212
BclI TGATCA 1 cut(s) 1672
BcnI CCSGG 4 cut(s) 713, 1551, 1686, 2206
BcoDI GTCTC 9 cut(s) 491, 1608, 1665, 1978, 2263, 2347, 2773, 2834, 2897
BfaI CTAG 6 cut(s) 704, 762, 1314, 1797, 2678, 2865
BfmI CTRYAG 3 cut(s) 135, 599, 870
BfrI CTTAAG 1 cut(s) 1649
BfuI GTATCC 3 cut(s) 32, 1714, 2212
BisI GCNGC 5 cut(s) 1335, 1574, 2144, 2169, 2937
BlnI CCTAGG 1 cut(s) 1313
BlsI GCNGC 5 cut(s) 1336, 1575, 2145, 2170, 2938
BmcAI AGTACT 2 cut(s) 1759, 1771
Bme1390I CCNGG 7 cut(s) 713, 1263, 1326, 1468, 1551, 1686, 2206
Bme18I GGWCC 1 cut(s) 1955
BmeRI GACNNNNNGTC 1 cut(s) 2446
BmeT110I CYCGRG 2 cut(s) 494, 671
BmgBI CACGTC 2 cut(s) 1445, 1997
BmgT120I GGNCC 2 cut(s) 300, 1955
BmiI GGNNCC 6 cut(s) 147, 669, 1233, 1465, 1891, 2086
BmrFI CCNGG 7 cut(s) 713, 1263, 1326, 1468, 1551, 1686, 2206
BmrI ACTGGG 4 cut(s) 20, 191, 365, 1070
BmsI GCATC 3 cut(s) 2018, 2757, 2938
BmuI ACTGGG 4 cut(s) 20, 191, 365, 1070
BpiI GAAGAC 2 cut(s) 1584, 2318
BplI GAGNNNNNCTC 2 cut(s) 1975, 2007
BpmI CTGGAG 1 cut(s) 900
Bpu10I CCTNAGC 4 cut(s) 1236, 1701, 1806, 1822
BpuEI CTTGAG 1 cut(s) 1989
BpuMI CCSGG 4 cut(s) 713, 1551, 1686, 2206
BsaBI GATNNNNATC 1 cut(s) 2625
BsaJI CCNNGG 9 cut(s) 672, 1215, 1261, 1313, 2159, 2264, 2348, 2699, 2886
BsaWI WCCGGW 2 cut(s) 1096, 1631
BsaXI ACNNNNNCTCC 4 cut(s) 377, 407, 958, 988
Bsc4I CCNNNNNNNGG 5 cut(s) 319, 385, 677, 875, 1325
Bse21I CCTNAGG 1 cut(s) 1008
Bse3DI GCAATG 1 cut(s) 2488
Bse8I GATNNNNATC 1 cut(s) 2625
BseAI TCCGGA 1 cut(s) 1096
BseBI CCWGG 3 cut(s) 1263, 1326, 1468
BseDI CCNNGG 9 cut(s) 672, 1215, 1261, 1313, 2159, 2264, 2348, 2699, 2886
BseGI GGATG 6 cut(s) 745, 845, 1817, 1898, 2027, 2036
BseJI GATNNNNATC 1 cut(s) 2625
BseLI CCNNNNNNNGG 5 cut(s) 319, 385, 677, 875, 1325
BseMI GCAATG 1 cut(s) 2488
BseRI GAGGAG 2 cut(s) 1241, 2148
BseXI GCAGC 4 cut(s) 1321, 1560, 2155, 2155
BseYI CCCAGC 1 cut(s) 875
BsgI GTGCAG 1 cut(s) 2162
BshFI GGCC 5 cut(s) 302, 728, 1318, 2846, 2885
BshNI GGYRCC 1 cut(s) 2084
BsiHKAI GWGCWC 2 cut(s) 462, 545
BsiHKCI CYCGRG 2 cut(s) 494, 671
BsiSI CCGG 6 cut(s) 713, 1097, 1550, 1632, 1686, 2206
BslI CCNNNNNNNGG 5 cut(s) 319, 385, 677, 875, 1325
BsmAI GTCTC 9 cut(s) 491, 1608, 1665, 1978, 2263, 2347, 2773, 2834, 2897
BsmBI CGTCTC 2 cut(s) 1978, 2834
BsmI GAATGC 2 cut(s) 130, 337
BsnI GGCC 5 cut(s) 302, 728, 1318, 2846, 2885
BsoBI CYCGRG 2 cut(s) 494, 671
Bsp1286I GDGCHC 4 cut(s) 462, 545, 672, 1092
Bsp13I TCCGGA 1 cut(s) 1096
Bsp19I CCATGG 1 cut(s) 1215
BspACI CCGC 3 cut(s) 503, 737, 2936
BspANI GGCC 5 cut(s) 302, 728, 1318, 2846, 2885
BspEI TCCGGA 1 cut(s) 1096
BspHI TCATGA 1 cut(s) 1084
BspLI GGNNCC 6 cut(s) 147, 669, 1233, 1465, 1891, 2086
BspQI GCTCTTC 2 cut(s) 1425, 2699
BspT107I GGYRCC 1 cut(s) 2084
BspTI CTTAAG 1 cut(s) 1649
BsrDI GCAATG 1 cut(s) 2488
BssECI CCNNGG 9 cut(s) 672, 1215, 1261, 1313, 2159, 2264, 2348, 2699, 2886
BssT1I CCWWGG 6 cut(s) 1215, 1313, 2264, 2348, 2699, 2886
Bst2UI CCWGG 3 cut(s) 1263, 1326, 1468
Bst6I CTCTTC 6 cut(s) 1008, 1425, 2699, 2832, 2864, 2922
BstAFI CTTAAG 1 cut(s) 1649
BstC8I GCNNGC 4 cut(s) 1863, 2334, 2848, 2852
BstDSI CCRYGG 1 cut(s) 1215
BstENI CCTNNNNNAGG 1 cut(s) 1323
BstF5I GGATG 6 cut(s) 745, 845, 1817, 1898, 2027, 2036
BstMAI GTCTC 9 cut(s) 491, 1608, 1665, 1978, 2263, 2347, 2773, 2834, 2897
BstMWI GCNNNNNNNGC 4 cut(s) 734, 1051, 2754, 2942
BstNI CCWGG 3 cut(s) 1263, 1326, 1468
BstNSI RCATGY 2 cut(s) 1865, 2955
BstSCI CCNGG 7 cut(s) 711, 1261, 1324, 1466, 1549, 1684, 2204
BstSFI CTRYAG 3 cut(s) 135, 599, 870
BstV1I GCAGC 4 cut(s) 1321, 1560, 2155, 2155
BstV2I GAAGAC 2 cut(s) 1584, 2318
BstX2I RGATCY 3 cut(s) 559, 800, 1889
BstXI CCANNNNNNTGG 3 cut(s) 1222, 2453, 2576
BstYI RGATCY 3 cut(s) 559, 800, 1889
Bsu36I CCTNAGG 1 cut(s) 1008
BsuI GTATCC 3 cut(s) 32, 1714, 2212
BsuRI GGCC 5 cut(s) 302, 728, 1318, 2846, 2885
BtgI CCRYGG 1 cut(s) 1215
BtrI CACGTC 2 cut(s) 1445, 1997
BtsCI GGATG 6 cut(s) 745, 845, 1817, 1898, 2027, 2036
BtsI GCAGTG 1 cut(s) 1507
Cac8I GCNNGC 4 cut(s) 1863, 2334, 2848, 2852
CaiI CAGNNNCTG 1 cut(s) 1331
CciI TCATGA 1 cut(s) 1084
Cfr13I GGNCC 2 cut(s) 300, 1955
Csp6I GTAC 3 cut(s) 1758, 1770, 2001
CviAII CATG 7 cut(s) 809, 1085, 1216, 1862, 2041, 2099, 2952
CviQI GTAC 3 cut(s) 1758, 1770, 2001
DraIII CACNNNGTG 1 cut(s) 1712
DriI GACNNNNNGTC 1 cut(s) 2446
EaeI YGGCCR 1 cut(s) 2844
Eam1104I CTCTTC 6 cut(s) 1008, 1425, 2699, 2832, 2864, 2922
Eam1105I GACNNNNNGTC 1 cut(s) 2446
EarI CTCTTC 6 cut(s) 1008, 1425, 2699, 2832, 2864, 2922
Ecl136II GAGCTC 2 cut(s) 460, 543
Eco130I CCWWGG 6 cut(s) 1215, 1313, 2264, 2348, 2699, 2886
Eco147I AGGCCT 2 cut(s) 1318, 2885
Eco24I GRGCYC 4 cut(s) 462, 545, 672, 1092
Eco32I GATATC 4 cut(s) 178, 532, 891, 1299
Eco47I GGWCC 1 cut(s) 1955
Eco53kI GAGCTC 2 cut(s) 460, 543
Eco57I CTGAAG 3 cut(s) 2228, 2312, 2903
Eco81I CCTNAGG 1 cut(s) 1008
Eco88I CYCGRG 2 cut(s) 494, 671
EcoICRI GAGCTC 2 cut(s) 460, 543
EcoNI CCTNNNNNAGG 1 cut(s) 1323
EcoO109I RGGNCCY 1 cut(s) 300
EcoRII CCWGG 3 cut(s) 1261, 1324, 1466
EcoRV GATATC 4 cut(s) 178, 532, 891, 1299
EcoT14I CCWWGG 6 cut(s) 1215, 1313, 2264, 2348, 2699, 2886
EcoT22I ATGCAT 1 cut(s) 2953
EcoT38I GRGCYC 4 cut(s) 462, 545, 672, 1092
ErhI CCWWGG 6 cut(s) 1215, 1313, 2264, 2348, 2699, 2886
Esp3I CGTCTC 2 cut(s) 1978, 2834
FaeI CATG 7 cut(s) 812, 1088, 1219, 1865, 2044, 2102, 2955
FalI AAGNNNNNCTT 4 cut(s) 1901, 1933, 2310, 2342
FatI CATG 7 cut(s) 808, 1084, 1215, 1861, 2040, 2098, 2951
FbaI TGATCA 1 cut(s) 1672
Fnu4HI GCNGC 5 cut(s) 1335, 1574, 2144, 2169, 2937
FokI GGATG 6 cut(s) 752, 852, 1804, 1885, 2014, 2023
FriOI GRGCYC 4 cut(s) 462, 545, 672, 1092
Fsp4HI GCNGC 5 cut(s) 1335, 1574, 2144, 2169, 2937
FspBI CTAG 6 cut(s) 704, 762, 1314, 1797, 2678, 2865
GluI GCNGC 5 cut(s) 1335, 1574, 2144, 2169, 2937
GsaI CCCAGC 1 cut(s) 879
GsuI CTGGAG 1 cut(s) 900
HaeIII GGCC 5 cut(s) 302, 728, 1318, 2846, 2885
HapII CCGG 6 cut(s) 713, 1097, 1550, 1632, 1686, 2206
Hin1II CATG 7 cut(s) 812, 1088, 1219, 1865, 2044, 2102, 2955
HincII GTYRAC 1 cut(s) 2451
HindII GTYRAC 1 cut(s) 2451
HindIII AAGCTT 3 cut(s) 1852, 1915, 2852
HpaII CCGG 6 cut(s) 713, 1097, 1550, 1632, 1686, 2206
HphI GGTGA 9 cut(s) 185, 239, 536, 692, 1078, 1565, 1806, 2245, 2573
Hpy166II GTNNAC 3 cut(s) 1442, 2064, 2451
Hpy8I GTNNAC 3 cut(s) 1442, 2064, 2451
Hpy99I CGWCG 3 cut(s) 1998, 2299, 2383
HpyCH4IV ACGT 3 cut(s) 1444, 1924, 1996
HpyCH4V TGCA 7 cut(s) 647, 819, 1576, 1865, 2143, 2171, 2951
HpyF10VI GCNNNNNNNGC 4 cut(s) 734, 1051, 2754, 2942
HpySE526I ACGT 3 cut(s) 1444, 1924, 1996
Hsp92II CATG 7 cut(s) 812, 1088, 1219, 1865, 2044, 2102, 2955
Kpn2I TCCGGA 1 cut(s) 1096
Ksp22I TGATCA 1 cut(s) 1672
LguI GCTCTTC 2 cut(s) 1425, 2699
LmnI GCTCC 4 cut(s) 457, 540, 667, 1934
Lsp1109I GCAGC 4 cut(s) 1321, 1560, 2155, 2155
LweI GCATC 3 cut(s) 2018, 2757, 2938
MaeI CTAG 6 cut(s) 704, 762, 1314, 1797, 2678, 2865
MaeII ACGT 3 cut(s) 1444, 1924, 1996
MaeIII GTNAC 8 cut(s) 56, 373, 680, 928, 952, 1156, 1198, 2501
MfeI CAATTG 1 cut(s) 2172
MflI RGATCY 3 cut(s) 559, 800, 1889
MhlI GDGCHC 4 cut(s) 462, 545, 672, 1092
MmeI TCCRAC 2 cut(s) 229, 258
Mph1103I ATGCAT 1 cut(s) 2953
MroI TCCGGA 1 cut(s) 1096
MroXI GAANNNNTTC 3 cut(s) 130, 970, 1174
MseI TTAA 7 cut(s) 1119, 1407, 1650, 1929, 2688, 2711, 2823
MslI CAYNNNNRTG 3 cut(s) 950, 1668, 2787
MspA1I CMGCKG 3 cut(s) 1826, 1952, 2936
MspCI CTTAAG 1 cut(s) 1649
MspI CCGG 6 cut(s) 713, 1097, 1550, 1632, 1686, 2206
MspR9I CCNGG 7 cut(s) 713, 1263, 1326, 1468, 1551, 1686, 2206
MunI CAATTG 1 cut(s) 2172
Mva1269I GAATGC 2 cut(s) 130, 337
MvaI CCWGG 3 cut(s) 1263, 1326, 1468
MwoI GCNNNNNNNGC 4 cut(s) 734, 1051, 2754, 2942
NciI CCSGG 4 cut(s) 713, 1551, 1686, 2206
NcoI CCATGG 1 cut(s) 1215
NlaIII CATG 7 cut(s) 812, 1088, 1219, 1865, 2044, 2102, 2955
NlaIV GGNNCC 6 cut(s) 147, 669, 1233, 1465, 1891, 2086
NmuCI GTSAC 6 cut(s) 56, 373, 680, 928, 952, 1156
NsiI ATGCAT 1 cut(s) 2953
NspI RCATGY 2 cut(s) 1865, 2955
PaeI GCATGC 1 cut(s) 1865
PagI TCATGA 1 cut(s) 1084
PceI AGGCCT 2 cut(s) 1318, 2885
PciSI GCTCTTC 2 cut(s) 1425, 2699
PcsI WCGNNNNNNNCGW 1 cut(s) 1993
PctI GAATGC 2 cut(s) 130, 337
PdmI GAANNNNTTC 3 cut(s) 130, 970, 1174
PfeI GAWTC 7 cut(s) 920, 1100, 1211, 1751, 1778, 1940, 2598
PfoI TCCNGGA 2 cut(s) 1684, 2204
PkrI GCNGC 5 cut(s) 1336, 1575, 2145, 2170, 2938
PsiI TTATAA 1 cut(s) 1644
Psp124BI GAGCTC 2 cut(s) 462, 545
Psp6I CCWGG 3 cut(s) 1261, 1324, 1466
PspFI CCCAGC 1 cut(s) 875
PspGI CCWGG 3 cut(s) 1261, 1324, 1466
PspN4I GGNNCC 6 cut(s) 147, 669, 1233, 1465, 1891, 2086
PspPI GGNCC 2 cut(s) 300, 1955
PstNI CAGNNNCTG 1 cut(s) 1331
PsuI RGATCY 3 cut(s) 559, 800, 1889
PvuII CAGCTG 2 cut(s) 1826, 1952
RsaI GTAC 3 cut(s) 1759, 1771, 2002
RsaNI GTAC 3 cut(s) 1758, 1770, 2001
RseI CAYNNNNRTG 3 cut(s) 950, 1668, 2787
SacI GAGCTC 2 cut(s) 462, 545
SapI GCTCTTC 2 cut(s) 1425, 2699
SaqAI TTAA 7 cut(s) 1119, 1407, 1650, 1929, 2688, 2711, 2823
SatI GCNGC 5 cut(s) 1335, 1574, 2144, 2169, 2937
Sau96I GGNCC 2 cut(s) 300, 1955
ScaI AGTACT 2 cut(s) 1759, 1771
ScrFI CCNGG 7 cut(s) 713, 1263, 1326, 1468, 1551, 1686, 2206
SduI GDGCHC 4 cut(s) 462, 545, 672, 1092
SfaNI GCATC 3 cut(s) 2018, 2757, 2938
SfcI CTRYAG 3 cut(s) 135, 599, 870
SinI GGWCC 1 cut(s) 1955
SmiMI CAYNNNNRTG 3 cut(s) 950, 1668, 2787
SmlI CTYRAG 2 cut(s) 1649, 2004
SmoI CTYRAG 2 cut(s) 1649, 2004
SphI GCATGC 1 cut(s) 1865
SseBI AGGCCT 2 cut(s) 1318, 2885
SsiI CCGC 3 cut(s) 503, 737, 2936
SspMI CTAG 6 cut(s) 704, 762, 1314, 1797, 2678, 2865
SstI GAGCTC 2 cut(s) 462, 545
StuI AGGCCT 2 cut(s) 1318, 2885
StyD4I CCNGG 7 cut(s) 711, 1261, 1324, 1466, 1549, 1684, 2204
StyI CCWWGG 6 cut(s) 1215, 1313, 2264, 2348, 2699, 2886
TaiI ACGT 3 cut(s) 1447, 1927, 1999
TatI WGTACW 2 cut(s) 1757, 1769
TauI GCSGC 1 cut(s) 2939
TfiI GAWTC 7 cut(s) 920, 1100, 1211, 1751, 1778, 1940, 2598
Tru1I TTAA 7 cut(s) 1119, 1407, 1650, 1929, 2688, 2711, 2823
Tru9I TTAA 7 cut(s) 1119, 1407, 1650, 1929, 2688, 2711, 2823
TseFI GTSAC 6 cut(s) 56, 373, 680, 928, 952, 1156
TseI GCWGC 4 cut(s) 1334, 1573, 2143, 2168
Tsp45I GTSAC 6 cut(s) 56, 373, 680, 928, 952, 1156
TspDTI ATGAA 4 cut(s) 246, 1013, 1407, 2043
TspGWI ACGGA 2 cut(s) 470, 1576
Vha464I CTTAAG 1 cut(s) 1649
VpaK11BI GGWCC 1 cut(s) 1955
XagI CCTNNNNNAGG 1 cut(s) 1323
XapI RAATTY 3 cut(s) 2276, 2360, 2660
XceI RCATGY 2 cut(s) 1865, 2955
XmaJI CCTAGG 1 cut(s) 1313
XmnI GAANNNNTTC 3 cut(s) 130, 970, 1174
XspI CTAG 6 cut(s) 704, 762, 1314, 1797, 2678, 2865
ZrmI AGTACT 2 cut(s) 1759, 1771
Zsp2I ATGCAT 1 cut(s) 2953
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.