MD14G1207900.v1.1

Plant calmodulin-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0012620
Physical Location & Seq
Forward (+)
29476415 .. 29481014
4600 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1207900.v1.1.491

Sequence Viewer

Length: 3294 bp
ATGGAAGTAGAGAACATCGGCATCCCCGTGATCCTACAAGTCCTGGACCTTCGTGGTGACAAGGGCAAAAATAATTCGATCCCACAAGAACCAGAGTCCAATGGGGGAGATGTTGACAGTAATATCAGAAGGGATTCCATCCCTGAAATCCCACCAGAAATGGATCTCAAGGGTGGTGATGTCAAAGGTAACTCTGTCCTAGGGGTTCCACAGGACATAGAGCCCAATGGCAGTGATAGCAGAAGGAGCTCTATCGGAAATGTTGATCCTCCTCATATCAAAAGGAGCTCTATCCCTGTGATCCCACAGGAAATGGATCCCAAGGGTGGTGATGTCGAAGGTAATTCGGTCGTAGGGATTCCACAGGACAGGGAGCCCAATGGTGGTGACATCAGAAGGAGCGCTATCGGAAATGTTGATTCTCCTTCTACCGAGAGAAACTCTAACCCAGTGGTCCCGCAGGACATGGACCTCAGTGATGATGATAGGAACTTTATTGCAAATGTCGCACGGGAAATAGAACCCGATGGCAGTGCTATCAAACTGAACTCTATCCTAGAGATCCCACAGAAAAAGGAGCCTGAGGGTGGAAATATCAGAAGCAAGTCTATCCTAGAGAACCCACAGGAAATGGAGTCTAATGCCGGTGATATCAGAAGAAGCTCCATCGAGAAAGTAGATCCTCCTGCTGATATCAAAAGGACCTCTATCCCAGTGATCCCACGGGAAATGGAGCCTAATGGTGGGGATGTTAGAAGGAACTCTATCAGAGTGATCCCACAGGAATTAGAACCCAATGGTGGTGGTATCGGAAGGAACTCTATGCCAGCGGTCCCACAGAAAATGGAACCAAATGCTGGGGATACCAGCAGGAACTCTGTTCTGTGGATCCCACATGAAATGGAACCAAAGGGTGGTAGAAGGATTCAGTCCCGCTATCTCAGAGTTCCAATAGGTTCTTGCCATGATTATTGCAAATATGGAGTGAGAAATGCATCTGAAGAAAATGCAACTGCTGCCATGCGAAAATCGGCTAAAGAGAGGAAACAGATATCCACAACCAATCAAAAGCCTTCTCCAGATTCCGACACCCAGAGGCTTGACAATTCTCTTGTCACAGAGAAAAAAGTTTTGTCATTGAGGAAGAAGGAAATTGTTTCTGCAGAAAAGGTTCCATCCTCTTCCAAGGATATTGATGTTTCTGTGGAAGATTCCTCCAGTTTGAAGGTGAAGCGTGTGAAATCAGAGCTATCTTCTCTCCCAGAACCGGCCTTAAGCCTGAGAAATTCAAAGGCAATATCCACAACTGGACCGAGGTCTGTTCTACATTCCCAAACCTTGAAACTTAATAATTCTGTCGTCACGGAGAAAAAAGGTTCATCATCGACGAAGAAAGAAACTGCTTCTTCAAAGAAAGTTTCATCTCCCAAGGAAATAGCCGTTTCTATGGAAGAGTTGATTGATTCGAAAATGAAGGCTGAGCAATTAGATCAATCTTCTCTCCCAGGATCAGACCCAAGCCTGAGCAATTCGGAGGTGACATCGACAATCAATCCTAAGCCTTCTCCAGATTCCGAGTCCCAGAATTTTATTGCCACGCAGAAAAGAGTTTCACCTCCCTATAAAGATATAGATGTTTCTGCTGAAGATTCCAACGATTCAAAAGTGATTTCAACAGTCAGTCCTAATCCTTCTCCAGATTCTAAAGCACGGAAACTTAATCATTCTGGTGTCATGGAGAAAAGAGCTTTATCATCAAAAAGGAAAGAAAATGTCTCTTCAAAAACGGTTTTAACTCCCATTAAGGAGATTGAAGTTTCTATGGAAGGTTCCATCGGTTCAAAGGTGAAGCGTGAGCAAGTAGAGTCATCTTCTTGCACAGGACAAGGTTCAAGTATTCAAGGAAAGAGTGGAACTAGAAAAGGCAAGGAAATACCCGAACGTTTGAGCAGCAGTGTCAATAGAAAAAACAGAAGCAAACCGCCGAGGACTCCTGTATTGGTTGAGAAAAAGATGTTAGAGCCTGAAACTGTTTCTTTGACTTACAAACACCCCGTCAAGAGAGTCTGCGATGAAAATGCTGGAAGCTTTAAGAACCTTAAAGGGTTGTCTCATATGAAAGATCAAAACGGTCCCCGAAAAGTTGAACCTGAGGGACCCTGCAATAAGGATGTTCCAGAAAAGATTTTGTATGTCATCGAGTCTAATACTGAGAATAGTACTGTCGAACCAACTCCAAATGGTGCCGATGCCCCTAAGTTATCGTCGTCGTCACATCAATCCTCGGAAGTCAAAAGCTCGAGACAAGCTAGGAAGGGAGTTGGTACCACTCGAGCACCGACATCCTCTATGAAGAAAAACTTGAAACGCACTGCAAATGGAGGTTATGGTCGTTCACCTACTGTTCCATCCCCTAAAAACAAAGGCTTGAGGCGTAGTACTCGAAGACCTTTATCTTCATCGTCATCCTTATCATCATCATCCATATCATCATCATCCGTGTCCGCATCCCCAGTCAAGTCCACTCATAAAAAAGAAAATGGCGCCACCTCTCAACGCAAAGCAATGAAAACTGGTAATCAAAGAGCAAATTTGAAGGTGGGGAAGAGTACTAGGCCTCGAAAGTCTGAGATACTTAGCTCTGAAAGCAAAAACAGCTCAGCCCGAAAGCTGACATTTAGGAGAGGAAGGGTGCTTGACATTAAGCCTGAGATCAATACTACAAGGAGACTCAAATTCAGGCGAGTAAGATTGGCAGGGGAGACCCAAAATGGTAAAGGTGGTGTAATAGGGGGAAGCGCCGGGAGGAAAGAAGTTGGTGACAGCCAGTCAATTGGTGATGTATTCCAAAGGTTAAGCCCCTGGTGGAAAGTAGTTGATCACAGTCACTCAAATGGTGCTTTTATCAAAAGGCGAAGCCTTGGGAGGATTGAAGTTGATGGCAGCCAGTCAAATGGTGGTAAATTCAAAAGCGAGAAAGTTGTTCCGAGAAGCTTCAAACGAGATGTGGAAGGTTCACAAGGGAAGAGCTTGAGGAGGAAAGCGGCTGGTGATAGTGGGTTGAATGGTAGTACTAAAACGAGTCCTGAGAAAGTTGTGTTGAGACACCAAGGTGTGACAGGAAGGAAGGATGTGCAGAAGTTGTTGAATAATGTGATTGAAGAGACTGCCAGTAGGCTTGTTGAGTCCAGGAAGAGTAAAGTCAAGGCCTTGGTTGGTGCTTTCGAGACTGTGATTTCGCTTCAGGATACCAAATCCATGGCATCGGAAACAGACACAGAAGATGCAGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaM_binding

Protein Analysis

1098

Amino Acids

118.99

Weight (kDa)

9.78

Isoelectric Point (pI)

62.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CaM_binding PF07839 1000 - 1080 2.3e-26 Plant calmodulin-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 2324
AccB1I GGYRCC 3 cut(s) 2243, 2324, 2543
AccB7I CCANNNNNTGG 2 cut(s) 857, 914
AciI CCGC 6 cut(s) 458, 830, 934, 1982, 2505, 3044
AclI AACGTT 1 cut(s) 1942
AcsI RAATTY 5 cut(s) 1285, 1585, 2590, 2735, 2963
AcuI CTGAAG 3 cut(s) 1020, 1665, 3227
AcyI GRCGYC 1 cut(s) 2544
AfaI GTAC 5 cut(s) 2221, 2326, 2440, 2611, 3073
AfeI AGCGCT 1 cut(s) 403
AfiI CCNNNNNNNGG 8 cut(s) 326, 383, 587, 743, 800, 857, 914, 1267
AflII CTTAAG 1 cut(s) 1273
AhdI GACNNNNNGTC 1 cut(s) 2827
AjnI CCWGG 4 cut(s) 42, 1504, 2861, 3188
AleI CACNNNNGTG 1 cut(s) 3111
Alw21I GWGCWC 3 cut(s) 251, 290, 2338
Alw26I GTCTC 8 cut(s) 1780, 2115, 2296, 2722, 2756, 3097, 3158, 3221
Ama87I CYCGRG 2 cut(s) 2299, 2331
Aor51HI AGCGCT 1 cut(s) 403
AoxI GGCC 3 cut(s) 1269, 2615, 3207
ApeKI GCWGC 3 cut(s) 1016, 1950, 2943
ApoI RAATTY 5 cut(s) 1285, 1585, 2590, 2735, 2963
ArsI GACNNNNNNTTYG 4 cut(s) 2040, 2072, 3220, 3252
Asp700I GAANNNNTTC 2 cut(s) 133, 1170
Asp718I GGTACC 1 cut(s) 2324
AspA2I CCTAGG 1 cut(s) 199
AspLEI GCGC 3 cut(s) 404, 2546, 2801
AspS9I GGNCC 8 cut(s) 46, 454, 469, 702, 832, 1310, 2132, 2156
AsuC2I CCSGG 1 cut(s) 2803
AsuII TTCGAA 1 cut(s) 1466
AvaI CYCGRG 2 cut(s) 2299, 2331
AvaII GGWCC 8 cut(s) 46, 454, 469, 702, 832, 1310, 2132, 2156
AvrII CCTAGG 1 cut(s) 199
AxyI CCTNAGG 2 cut(s) 582, 2151
BamHI GGATCC 2 cut(s) 316, 888
BanI GGYRCC 3 cut(s) 2243, 2324, 2543
BanII GRGCYC 4 cut(s) 225, 251, 290, 378
BbsI GAAGAC 1 cut(s) 2452
Bbv12I GWGCWC 3 cut(s) 251, 290, 2338
BbvI GCAGC 3 cut(s) 1003, 1962, 2955
BccI CCATC 7 cut(s) 146, 521, 674, 1183, 1841, 2416, 2933
BceAI ACGGC 1 cut(s) 1424
BcgI CGANNNNNNTGC 4 cut(s) 515, 549, 2060, 2094
BciT130I CCWGG 4 cut(s) 44, 1506, 2863, 3190
BciVI GTATCC 2 cut(s) 856, 3241
BclI TGATCA 1 cut(s) 2878
BcnI CCSGG 1 cut(s) 2803
BcoDI GTCTC 8 cut(s) 1780, 2115, 2296, 2722, 2756, 3097, 3158, 3221
BfaI CTAG 6 cut(s) 200, 557, 614, 1917, 2310, 2613
BfmI CTRYAG 1 cut(s) 1161
BfoI RGCGCY 3 cut(s) 405, 2547, 2802
BfrI CTTAAG 1 cut(s) 1273
BfuI GTATCC 2 cut(s) 856, 3241
BisI GCNGC 4 cut(s) 1017, 1951, 2944, 3045
BlnI CCTAGG 1 cut(s) 199
BlpI GCTNAGC 2 cut(s) 1479, 2659
BlsI GCNGC 4 cut(s) 1018, 1952, 2945, 3046
BmcAI AGTACT 4 cut(s) 2221, 2440, 2611, 3073
Bme1390I CCNGG 5 cut(s) 44, 1506, 2803, 2863, 3190
Bme18I GGWCC 8 cut(s) 46, 454, 469, 702, 832, 1310, 2132, 2156
BmeRI GACNNNNNGTC 1 cut(s) 2827
BmeT110I CYCGRG 2 cut(s) 2299, 2331
BmgT120I GGNCC 8 cut(s) 46, 454, 469, 702, 832, 1310, 2132, 2156
BmrFI CCNGG 5 cut(s) 44, 1506, 2803, 2863, 3190
BmrI ACTGGG 3 cut(s) 443, 707, 2507
BmsI GCATC 6 cut(s) 30, 1004, 2239, 2516, 3272, 3274
BmuI ACTGGG 3 cut(s) 443, 707, 2507
BoxI GACNNNNGTC 1 cut(s) 1315
BpiI GAAGAC 1 cut(s) 2452
BplI GAGNNNNNCTC 2 cut(s) 425, 457
BpmI CTGGAG 4 cut(s) 1062, 1201, 1551, 1680
Bpu10I CCTNAGC 2 cut(s) 1523, 1557
Bpu1102I GCTNAGC 2 cut(s) 1479, 2659
Bpu14I TTCGAA 1 cut(s) 1466
BpuEI CTTGAG 3 cut(s) 152, 2449, 3052
BpuMI CCSGG 1 cut(s) 2803
BsaHI GRCGYC 1 cut(s) 2544
BsaI GGTCTC 1 cut(s) 2756
Bsc4I CCNNNNNNNGG 8 cut(s) 326, 383, 587, 743, 800, 857, 914, 1267
Bse118I RCCGGY 2 cut(s) 644, 1267
Bse1I ACTGG 9 cut(s) 449, 713, 1218, 1312, 2513, 2578, 2827, 2947, 3171
Bse21I CCTNAGG 2 cut(s) 582, 2151
Bse3DI GCAATG 1 cut(s) 2571
BseBI CCWGG 4 cut(s) 44, 1506, 2863, 3190
BseLI CCNNNNNNNGG 8 cut(s) 326, 383, 587, 743, 800, 857, 914, 1267
BseMI GCAATG 1 cut(s) 2571
BseNI ACTGG 9 cut(s) 449, 713, 1218, 1312, 2513, 2578, 2827, 2947, 3171
BseRI GAGGAG 2 cut(s) 261, 3049
BseXI GCAGC 3 cut(s) 1003, 1962, 2955
BseYI CCCAGC 1 cut(s) 857
BsgI GTGCAG 1 cut(s) 3155
Bsh1285I CGRYCG 1 cut(s) 351
BshFI GGCC 3 cut(s) 1271, 2617, 3209
BshNI GGYRCC 3 cut(s) 2243, 2324, 2543
BsiEI CGRYCG 1 cut(s) 351
BsiHKAI GWGCWC 3 cut(s) 251, 290, 2338
BsiHKCI CYCGRG 2 cut(s) 2299, 2331
BsiSI CCGG 3 cut(s) 645, 1268, 2802
BslFI GGGAC 6 cut(s) 440, 818, 916, 1564, 2118, 2169
BslI CCNNNNNNNGG 8 cut(s) 326, 383, 587, 743, 800, 857, 914, 1267
BsmAI GTCTC 8 cut(s) 1780, 2115, 2296, 2722, 2756, 3097, 3158, 3221
BsmFI GGGAC 6 cut(s) 440, 818, 916, 1564, 2118, 2169
BsnI GGCC 3 cut(s) 1271, 2617, 3209
Bso31I GGTCTC 1 cut(s) 2756
BsoBI CYCGRG 2 cut(s) 2299, 2331
Bsp119I TTCGAA 1 cut(s) 1466
Bsp1286I GDGCHC 5 cut(s) 225, 251, 290, 378, 2338
Bsp1720I GCTNAGC 2 cut(s) 1479, 2659
Bsp19I CCATGG 1 cut(s) 3258
BspACI CCGC 6 cut(s) 458, 830, 934, 1982, 2505, 3044
BspANI GGCC 3 cut(s) 1271, 2617, 3209
BspMAI CTGCAG 1 cut(s) 1165
BspQI GCTCTTC 1 cut(s) 3020
BspT104I TTCGAA 1 cut(s) 1466
BspT107I GGYRCC 3 cut(s) 2243, 2324, 2543
BspTI CTTAAG 1 cut(s) 1273
BspTNI GGTCTC 1 cut(s) 2756
BsrDI GCAATG 1 cut(s) 2571
BsrFI RCCGGY 2 cut(s) 644, 1267
BsrI ACTGG 9 cut(s) 449, 713, 1218, 1312, 2513, 2578, 2827, 2947, 3171
BssAI RCCGGY 2 cut(s) 644, 1267
BssNI GRCGYC 1 cut(s) 2544
BssT1I CCWWGG 8 cut(s) 199, 321, 1185, 1428, 2920, 3109, 3210, 3258
Bst2UI CCWGG 4 cut(s) 44, 1506, 2863, 3190
Bst4CI ACNGT 9 cut(s) 119, 1678, 1789, 2032, 2132, 2224, 2404, 2885, 3232
Bst6I CTCTTC 7 cut(s) 1186, 1446, 1783, 2600, 3020, 3156, 3188
BstACI GRCGYC 1 cut(s) 2544
BstAFI CTTAAG 1 cut(s) 1273
BstAPI GCANNNNNTGC 1 cut(s) 1016
BstBI TTCGAA 1 cut(s) 1466
BstC8I GCNNGC 1 cut(s) 828
BstDSI CCRYGG 2 cut(s) 722, 3258
BstH2I RGCGCY 3 cut(s) 405, 2547, 2802
BstHHI GCGC 3 cut(s) 404, 2546, 2801
BstMAI GTCTC 8 cut(s) 1780, 2115, 2296, 2722, 2756, 3097, 3158, 3221
BstMCI CGRYCG 1 cut(s) 351
BstMWI GCNNNNNNNGC 6 cut(s) 237, 246, 506, 1016, 2646, 2655
BstNI CCWGG 4 cut(s) 44, 1506, 2863, 3190
BstPAI GACNNNNGTC 1 cut(s) 1315
BstSCI CCNGG 5 cut(s) 42, 1504, 2801, 2861, 3188
BstSFI CTRYAG 1 cut(s) 1161
BstV1I GCAGC 3 cut(s) 1003, 1962, 2955
BstV2I GAAGAC 1 cut(s) 2452
BstX2I RGATCY 5 cut(s) 163, 316, 561, 679, 888
BstXI CCANNNNNNTGG 3 cut(s) 2834, 2954, 3259
BstYI RGATCY 5 cut(s) 163, 316, 561, 679, 888
Bsu36I CCTNAGG 2 cut(s) 582, 2151
BsuI GTATCC 2 cut(s) 856, 3241
BsuRI GGCC 3 cut(s) 1271, 2617, 3209
BtgI CCRYGG 2 cut(s) 722, 3258
BtgZI GCGATG 1 cut(s) 2085
BtsI GCAGTG 4 cut(s) 238, 538, 1960, 2370
BtsIMutI CAGTG 7 cut(s) 238, 456, 481, 538, 720, 1960, 2370
Cac8I GCNNGC 1 cut(s) 828
CfoI GCGC 3 cut(s) 404, 2546, 2801
Cfr10I RCCGGY 2 cut(s) 644, 1267
Cfr13I GGNCC 8 cut(s) 46, 454, 469, 702, 832, 1310, 2132, 2156
Csp6I GTAC 5 cut(s) 2220, 2325, 2439, 2610, 3072
CspCI CAANNNNNGTGG 2 cut(s) 784, 819
CviAII CATG 6 cut(s) 466, 896, 965, 1021, 1735, 3259
CviQI GTAC 5 cut(s) 2220, 2325, 2439, 2610, 3072
DinI GGCGCC 1 cut(s) 2545
DriI GACNNNNNGTC 1 cut(s) 2827
Eam1104I CTCTTC 7 cut(s) 1186, 1446, 1783, 2600, 3020, 3156, 3188
Eam1105I GACNNNNNGTC 1 cut(s) 2827
EarI CTCTTC 7 cut(s) 1186, 1446, 1783, 2600, 3020, 3156, 3188
Ecl136II GAGCTC 2 cut(s) 249, 288
Eco130I CCWWGG 8 cut(s) 199, 321, 1185, 1428, 2920, 3109, 3210, 3258
Eco147I AGGCCT 2 cut(s) 2617, 3209
Eco24I GRGCYC 4 cut(s) 225, 251, 290, 378
Eco31I GGTCTC 1 cut(s) 2756
Eco32I GATATC 3 cut(s) 652, 694, 1053
Eco47I GGWCC 8 cut(s) 46, 454, 469, 702, 832, 1310, 2132, 2156
Eco47III AGCGCT 1 cut(s) 403
Eco53kI GAGCTC 2 cut(s) 249, 288
Eco57I CTGAAG 3 cut(s) 1020, 1665, 3227
Eco81I CCTNAGG 2 cut(s) 582, 2151
Eco88I CYCGRG 2 cut(s) 2299, 2331
EcoICRI GAGCTC 2 cut(s) 249, 288
EcoO109I RGGNCCY 2 cut(s) 702, 2156
EcoRII CCWGG 4 cut(s) 42, 1504, 2861, 3188
EcoRV GATATC 3 cut(s) 652, 694, 1053
EcoT14I CCWWGG 8 cut(s) 199, 321, 1185, 1428, 2920, 3109, 3210, 3258
EcoT22I ATGCAT 1 cut(s) 997
EcoT38I GRGCYC 4 cut(s) 225, 251, 290, 378
EgeI GGCGCC 1 cut(s) 2545
EheI GGCGCC 1 cut(s) 2545
ErhI CCWWGG 8 cut(s) 199, 321, 1185, 1428, 2920, 3109, 3210, 3258
FaeI CATG 6 cut(s) 469, 899, 968, 1024, 1738, 3262
FalI AAGNNNNNCTT 2 cut(s) 2345, 2377
FaqI GGGAC 6 cut(s) 440, 818, 916, 1564, 2118, 2169
FatI CATG 6 cut(s) 465, 895, 964, 1020, 1734, 3258
FauI CCCGC 2 cut(s) 465, 941
FauNDI CATATG 1 cut(s) 2115
FbaI TGATCA 1 cut(s) 2878
Fnu4HI GCNGC 4 cut(s) 1017, 1951, 2944, 3045
FriOI GRGCYC 4 cut(s) 225, 251, 290, 378
Fsp4HI GCNGC 4 cut(s) 1017, 1951, 2944, 3045
FspBI CTAG 6 cut(s) 200, 557, 614, 1917, 2310, 2613
GlaI GCGC 3 cut(s) 403, 2545, 2800
GluI GCNGC 4 cut(s) 1017, 1951, 2944, 3045
GsaI CCCAGC 1 cut(s) 861
GsuI CTGGAG 4 cut(s) 1062, 1201, 1551, 1680
HaeII RGCGCY 3 cut(s) 405, 2547, 2802
HaeIII GGCC 3 cut(s) 1271, 2617, 3209
HapII CCGG 3 cut(s) 645, 1268, 2802
HhaI GCGC 3 cut(s) 404, 2546, 2801
Hin1I GRCGYC 1 cut(s) 2544
Hin1II CATG 6 cut(s) 469, 899, 968, 1024, 1738, 3262
Hin6I GCGC 3 cut(s) 402, 2544, 2799
HinP1I GCGC 3 cut(s) 402, 2544, 2799
HincII GTYRAC 1 cut(s) 115
HindII GTYRAC 1 cut(s) 115
HindIII AAGCTT 2 cut(s) 2086, 2992
HpaII CCGG 3 cut(s) 645, 1268, 2802
Hpy166II GTNNAC 4 cut(s) 115, 2396, 2523, 3017
Hpy8I GTNNAC 4 cut(s) 115, 2396, 2523, 3017
Hpy99I CGWCG 3 cut(s) 1390, 2269, 2272
HpyCH4III ACNGT 9 cut(s) 119, 1678, 1789, 2032, 2132, 2224, 2404, 2885, 3232
HpyCH4IV ACGT 1 cut(s) 1942
HpyF10VI GCNNNNNNNGC 6 cut(s) 237, 246, 506, 1016, 2646, 2655
HpySE526I ACGT 1 cut(s) 1942
Hsp92I GRCGYC 1 cut(s) 2544
Hsp92II CATG 6 cut(s) 469, 899, 968, 1024, 1738, 3262
HspAI GCGC 3 cut(s) 402, 2544, 2799
KasI GGCGCC 1 cut(s) 2543
KflI GGGWCCC 1 cut(s) 2156
KpnI GGTACC 1 cut(s) 2328
Ksp22I TGATCA 1 cut(s) 2878
LguI GCTCTTC 1 cut(s) 3020
LmnI GCTCC 7 cut(s) 246, 285, 373, 399, 577, 668, 733
Lsp1109I GCAGC 3 cut(s) 1003, 1962, 2955
LweI GCATC 6 cut(s) 30, 1004, 2239, 2516, 3272, 3274
MaeI CTAG 6 cut(s) 200, 557, 614, 1917, 2310, 2613
MaeII ACGT 1 cut(s) 1942
MfeI CAATTG 1 cut(s) 2832
MflI RGATCY 5 cut(s) 163, 316, 561, 679, 888
MhlI GDGCHC 5 cut(s) 225, 251, 290, 378, 2338
Mly113I GGCGCC 1 cut(s) 2544
MmeI TCCRAC 2 cut(s) 1110, 1677
Mph1103I ATGCAT 1 cut(s) 997
MroXI GAANNNNTTC 2 cut(s) 133, 1170
MseI TTAA 9 cut(s) 1274, 1347, 1719, 1793, 1803, 2091, 2100, 2703, 2855
MslI CAYNNNNRTG 4 cut(s) 26, 1728, 2892, 3111
MspA1I CMGCKG 1 cut(s) 830
MspCI CTTAAG 1 cut(s) 1273
MspI CCGG 3 cut(s) 645, 1268, 2802
MspR9I CCNGG 5 cut(s) 44, 1506, 2803, 2863, 3190
MunI CAATTG 1 cut(s) 2832
MvaI CCWGG 4 cut(s) 44, 1506, 2863, 3190
MwoI GCNNNNNNNGC 6 cut(s) 237, 246, 506, 1016, 2646, 2655
NarI GGCGCC 1 cut(s) 2544
NciI CCSGG 1 cut(s) 2803
NcoI CCATGG 1 cut(s) 3258
NdeI CATATG 1 cut(s) 2115
NlaIII CATG 6 cut(s) 469, 899, 968, 1024, 1738, 3262
NmeAIII GCCGAG 1 cut(s) 2010
NmuCI GTSAC 9 cut(s) 56, 386, 1114, 1360, 1537, 2271, 2819, 2885, 3115
NsiI ATGCAT 1 cut(s) 997
NspV TTCGAA 1 cut(s) 1466
OliI CACNNNNGTG 1 cut(s) 3111
PaeR7I CTCGAG 2 cut(s) 2299, 2331
PceI AGGCCT 2 cut(s) 2617, 3209
PciSI GCTCTTC 1 cut(s) 3020
PcsI WCGNNNNNNNCGW 1 cut(s) 24
PdmI GAANNNNTTC 2 cut(s) 133, 1170
PflMI CCANNNNNTGG 2 cut(s) 857, 914
PfoI TCCNGGA 2 cut(s) 42, 3188
PkrI GCNGC 4 cut(s) 1018, 1952, 2945, 3046
PluTI GGCGCC 1 cut(s) 2547
PpuMI RGGWCCY 2 cut(s) 702, 2156
PshAI GACNNNNGTC 1 cut(s) 1315
Psp124BI GAGCTC 2 cut(s) 251, 290
Psp1406I AACGTT 1 cut(s) 1942
Psp5II RGGWCCY 2 cut(s) 702, 2156
Psp6I CCWGG 4 cut(s) 42, 1504, 2861, 3188
PspFI CCCAGC 1 cut(s) 857
PspGI CCWGG 4 cut(s) 42, 1504, 2861, 3188
PspPI GGNCC 8 cut(s) 46, 454, 469, 702, 832, 1310, 2132, 2156
PspPPI RGGWCCY 2 cut(s) 702, 2156
PspXI VCTCGAGB 1 cut(s) 2331
PstI CTGCAG 1 cut(s) 1165
PsuI RGATCY 5 cut(s) 163, 316, 561, 679, 888
RsaI GTAC 5 cut(s) 2221, 2326, 2440, 2611, 3073
RsaNI GTAC 5 cut(s) 2220, 2325, 2439, 2610, 3072
RseI CAYNNNNRTG 4 cut(s) 26, 1728, 2892, 3111
SacI GAGCTC 2 cut(s) 251, 290
SapI GCTCTTC 1 cut(s) 3020
SaqAI TTAA 9 cut(s) 1274, 1347, 1719, 1793, 1803, 2091, 2100, 2703, 2855
SatI GCNGC 4 cut(s) 1017, 1951, 2944, 3045
Sau96I GGNCC 8 cut(s) 46, 454, 469, 702, 832, 1310, 2132, 2156
ScaI AGTACT 4 cut(s) 2221, 2440, 2611, 3073
ScrFI CCNGG 5 cut(s) 44, 1506, 2803, 2863, 3190
SduI GDGCHC 5 cut(s) 225, 251, 290, 378, 2338
SfaNI GCATC 6 cut(s) 30, 1004, 2239, 2516, 3272, 3274
SfcI CTRYAG 1 cut(s) 1161
SfoI GGCGCC 1 cut(s) 2545
Sfr274I CTCGAG 2 cut(s) 2299, 2331
SfuI TTCGAA 1 cut(s) 1466
SinI GGWCC 8 cut(s) 46, 454, 469, 702, 832, 1310, 2132, 2156
SlaI CTCGAG 2 cut(s) 2299, 2331
SmiMI CAYNNNNRTG 4 cut(s) 26, 1728, 2892, 3111
SmlI CTYRAG 6 cut(s) 167, 1273, 2299, 2331, 2428, 3031
SmoI CTYRAG 6 cut(s) 167, 1273, 2299, 2331, 2428, 3031
SseBI AGGCCT 2 cut(s) 2617, 3209
SsiI CCGC 6 cut(s) 458, 830, 934, 1982, 2505, 3044
SspDI GGCGCC 1 cut(s) 2543
SspMI CTAG 6 cut(s) 200, 557, 614, 1917, 2310, 2613
SstI GAGCTC 2 cut(s) 251, 290
StuI AGGCCT 2 cut(s) 2617, 3209
StyD4I CCNGG 5 cut(s) 42, 1504, 2801, 2861, 3188
StyI CCWWGG 8 cut(s) 199, 321, 1185, 1428, 2920, 3109, 3210, 3258
TaaI ACNGT 9 cut(s) 119, 1678, 1789, 2032, 2132, 2224, 2404, 2885, 3232
TaiI ACGT 1 cut(s) 1945
TaqII GACCGA 2 cut(s) 337, 1327
TatI WGTACW 4 cut(s) 2219, 2438, 2609, 3071
TauI GCSGC 1 cut(s) 3047
Tru1I TTAA 9 cut(s) 1274, 1347, 1719, 1793, 1803, 2091, 2100, 2703, 2855
Tru9I TTAA 9 cut(s) 1274, 1347, 1719, 1793, 1803, 2091, 2100, 2703, 2855
TscAI CASTG 7 cut(s) 238, 456, 481, 538, 720, 1960, 2377
TseFI GTSAC 9 cut(s) 56, 386, 1114, 1360, 1537, 2271, 2819, 2885, 3115
TseI GCWGC 3 cut(s) 1016, 1950, 2943
Tsp45I GTSAC 9 cut(s) 56, 386, 1114, 1360, 1537, 2271, 2819, 2885, 3115
TspDTI ATGAA 9 cut(s) 912, 1368, 1410, 1487, 2088, 2132, 2366, 2448, 2582
TspGWI ACGGA 3 cut(s) 1379, 1726, 2488
TspRI CASTG 7 cut(s) 238, 456, 481, 538, 720, 1960, 2377
Van91I CCANNNNNTGG 2 cut(s) 857, 914
Vha464I CTTAAG 1 cut(s) 1273
VpaK11BI GGWCC 8 cut(s) 46, 454, 469, 702, 832, 1310, 2132, 2156
XapI RAATTY 5 cut(s) 1285, 1585, 2590, 2735, 2963
XcmI CCANNNNNNNNNTGG 1 cut(s) 2954
XhoI CTCGAG 2 cut(s) 2299, 2331
XmaJI CCTAGG 1 cut(s) 199
XmnI GAANNNNTTC 2 cut(s) 133, 1170
XspI CTAG 6 cut(s) 200, 557, 614, 1917, 2310, 2613
ZrmI AGTACT 4 cut(s) 2221, 2440, 2611, 3073
Zsp2I ATGCAT 1 cut(s) 997
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.