Rh7DG049200

Plant calmodulin-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0012620
Physical Location & Seq
Forward (+)
3800166 .. 3804813
4648 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG049200.1

Sequence Viewer

Length: 2880 bp
ATGGAGGTGGAGAATATCGGTATCCCAGTGATCCTACAAGACTTGAAGTTTATTTGTGACGATGGCAGAAGGAACTCTATCTCAGTGATTCCACAAGAAAAAGAACTCAATGGCAGTCACATTAGAAGGCATTCTATAGCACTGGTTCCACAGGAAATGGAGTCCACGCGTGGTGATATCATTAGCAACTCTATCCCTGTGATCCAACAGGAAATTGAGCCAAATGGTGATGAAATCGGGAGGAACTCTATTCCAGTGATCCCACAGGAAATCCAGTCGGATGGTGATGATTTCAGAAGGGCCTTTATTTTCCCACAGAAGGTGGAGTCTAATGCTGATGAGTCCGATGATGTTAGCACTAACTCAATCCCAGTGATCCCACAGGAGGTGGAGTCTAATGTTGATGAGTCCAATGATGTTAGCACAAACTCAATCCCAGTGATCCCACAGGAAATGGAGCTTGAAGGTAATGATATAGGAAGGAACTCTATCCCCGTGACTCCGCAGGAAATAGAGCTTGATGGTGATGATATCAGAAGGAGCTCTATTGGGGAAGTAGATCCAGTTGATATACAAACTAACTCTACAGTAATCCTACAGAAAATTGAGTCCAATGGTGGTGATGTGAGAAGGAATTCCATTGCAGGTATCCCACAGGAAATAGAGTCCGAGGATGGTGACATCAGAAGGAACTCTATCATAGTGATCCCGGTGGGAATAGAGGCCAGTGGTGCTGATGTCAGAAGGAGGTCTATTGCTAGAAATATCCAATCCCGTTATCTCAGAGCTTCAATGGGATCTTGCCATGATTATTGCAAATATGGAATAAGGGAAGGATGTGAAGATGAGAATTTGGAAATATCTCCTATAGCTGGGAGGAAAAGGATATCCACAATCAACTCTAAGCCTTCTCCAGATTCTTTTGTCACAAAGAAAAGGGTCATATCAGTGACGAAGAAAGGAACTCCTTCTCCTAAAAAAGTTTCTAAGGAAGTTGGTGTTCCTAAGGAAGAGTCCATTCATTTGAGGAAAAAGCGTGGGCAATTTGAGCCATCTTCTGAGGTGAAATCCCCAGTCAGTCATGAGCCTTCTCCGGATTCAGAAGCCCACATACTTAACAAGTCTTTTGTCACAGAGAAAAGGGTTGTGTCAGTGACAAAGAAAGGAACTGTTTCTTTGGAAAAAGAGATTGATGTTACTATGGAAGATTCCATGGATTTGGGGGAGGAACCTGAGCAATTAGAGTCATATTCTCTCCCAGGATCAGTCCAAAGCCTGAGAAATTCGAAAGTGATATCCACAGTCAGTCCTAGGCCTTATCCAGGTTCTGCTGCTATGGAGAAGAAAGTTATATCAGTGAAAAAGAAAGATACTGCTTCTTCAAAAAATGAAATGCCTTCCTTAAAGGAAATTTATGTTTCTATGGAAGAGCAAGGTTTACGTCATAAAAGAAACATTGGAACCAGAAATAGCAAGGAAGCACCTGACGGTTCAAGCAGTGGAGGAAATAGAAGTATCAGAAACCATAATAGGAGGGCTTCTCTACCGGGTGAGAAACAGACAATGGGGCTGCAACATGTTTCTTTGTCTTCCAAACACTTCAATAAGAGAGACTCCAATGTGAATACCGGAAGCTCTTATAACCTCAAGGGGTTGACTCATCTGAAAGATCAAAATGATCCCGGAAAAGTTGAACCTCAGCCTCCAAGTGATAAAGAAACGCCAGAAAAGATTTTGTATGTCATTGAATCAAGTACTGAGAATGGTACCATGGAATCAACTCCAAATGGTGTTAGTGTTCCTGAGCCATCACCATCCTCAGCTGTATCTGTTGAAGGCAAAGGCAAAAGCTTGAAGCATGCAAGGAAGGGAACTGGCAGAACTGGATCCTCTCCATCCTCTGAGAAGAAAAACTTGAAACGTGTTGTTAATGGAGCTGATTCTCGATTAGCTGGTCCTTTAGATCGGGATAACAAGGGGTTGAGAAGAACTCGACGTGGTACTCCAGCATCTTTATCTCCATCGTCATCCTTATCATCCATGTCCATATATTCATCTGACTCCACTCAACATAAAGAAAATGGTGCCCACTCTCAGCATGATAGAAGTAAAAATGTGAAGCCAATAGGAAAGTCGAAGGTGCAGCACAAGACTACTCCTCGGAGGGCTGCAATTGTTGGCTCTGGAAACAAAAACAGTGTATCCCGGAAGCTGACATTTCAGAGAGGTAGGGTGATTGAACTTCAGCCTGAGAACAATACTCCAAGGAGACTCAAATTCAGGCGAGTAAGATCGGCAATGGAGGTTCCAAACAGTAAAGGCAGTATCACAAGTGGAAGAATTGGGAGGAAGGAAGTTGATGACATCCAGTCAACTGGTGCTGGTATCATCAAAAAGGGAAGCAATGTGAGGAAGGAAGTTGGTAACAGACAGTCAAGTGGTGCTGGTATCAAAACGGTAAGCATTGTGAGGAAGGAAGTTGGTGATACCCAGTCAAGTGGTGTTGCTATCAAAAGGGGAATCATTGGGAGGAAGGAAGTTTATGACAATCAGACAAATGGTGCTAAACTCAAATCCGAGAAAGTTGTTCTGAGAAACCAAGAACAAGTTGAAGTTTCCTCAAGGAAGAGCTTAACGAGGAAGGGGGTTGCTGTTGGTCTTTTGAATGGCATCAGAAGCAATCCTGAGAAAGTGGTTTTGAGACACCAAGATGTAAAAGGAAAGAAAGGTGATCAGAAATTGTTCAACAATGTGATTGAAGAGACGGCCAGCAAGCTTGTCGAAACTAGGAAGAGTAAAGTCAAGGCCTTGGTTGGTGCTTTTGAGACTGTGATCTCTCTTCAGGACACCAGACCAGCGGCAGCGGCAGATGCATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaM_binding

Protein Analysis

959

Amino Acids

104.79

Weight (kDa)

9.5

Isoelectric Point (pI)

60.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CaM_binding PF07839 850 - 946 6.5e-26 Plant calmodulin-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1641
Acc36I ACCTGC 1 cut(s) 635
Acc65I GGTACC 1 cut(s) 1766
AccB1I GGYRCC 2 cut(s) 1766, 2084
AccII CGCG 1 cut(s) 169
AccIII TCCGGA 1 cut(s) 1093
AciI CCGC 3 cut(s) 503, 2858, 2864
AcoI YGGCCR 1 cut(s) 2766
AcsI RAATTY 5 cut(s) 634, 850, 1282, 1410, 2276
AcuI CTGAAG 2 cut(s) 2228, 2825
AfaI GTAC 3 cut(s) 1756, 1768, 2002
AfiI CCNNNNNNNGG 4 cut(s) 319, 385, 872, 1322
AflIII ACRYGT 3 cut(s) 167, 1576, 1921
AhdI GACNNNNNGTC 1 cut(s) 2368
AjiI CACGTC 1 cut(s) 1997
AjnI CCWGG 2 cut(s) 1258, 1321
AloI GAACNNNNNNTCC 2 cut(s) 955, 987
Alw21I GWGCWC 1 cut(s) 545
Alw26I GTCTC 5 cut(s) 1605, 2263, 2695, 2756, 2819
AlwNI CAGNNNCTG 1 cut(s) 1328
Aor13HI TCCGGA 1 cut(s) 1093
AoxI GGCC 5 cut(s) 300, 723, 1313, 2766, 2805
ApeKI GCWGC 5 cut(s) 1331, 1570, 2143, 2168, 2861
ApoI RAATTY 5 cut(s) 634, 850, 1282, 1410, 2276
Asp700I GAANNNNTTC 5 cut(s) 130, 634, 967, 1171, 2741
Asp718I GGTACC 1 cut(s) 1766
AspA2I CCTAGG 1 cut(s) 1310
AspS9I GGNCC 2 cut(s) 300, 1955
AsuC2I CCSGG 4 cut(s) 710, 1548, 1683, 2206
AsuII TTCGAA 1 cut(s) 1286
AvaII GGWCC 1 cut(s) 1955
AvrII CCTAGG 1 cut(s) 1310
AxyI CCTNAGG 1 cut(s) 1005
BaeGI GKGCMC 1 cut(s) 2089
BamHI GGATCC 1 cut(s) 1886
BanI GGYRCC 2 cut(s) 1766, 2084
BanII GRGCYC 1 cut(s) 545
BarI GAAGNNNNNNTAC 2 cut(s) 1095, 1127
BbsI GAAGAC 1 cut(s) 1581
Bbv12I GWGCWC 1 cut(s) 545
BbvCI CCTCAGC 2 cut(s) 1698, 1819
BbvI GCAGC 5 cut(s) 1318, 1557, 2155, 2155, 2873
BccI CCATC 9 cut(s) 56, 275, 515, 668, 1060, 1816, 1822, 1903, 2029
BceAI ACGGC 1 cut(s) 2781
BcgI CGANNNNNNTGC 2 cut(s) 2761, 2795
BciT130I CCWGG 2 cut(s) 1260, 1323
BciVI GTATCC 3 cut(s) 32, 659, 2212
BclI TGATCA 1 cut(s) 2731
BcnI CCSGG 4 cut(s) 710, 1548, 1683, 2206
BcoDI GTCTC 5 cut(s) 1605, 2263, 2695, 2756, 2819
BfaI CTAG 3 cut(s) 759, 1311, 2787
BfmI CTRYAG 4 cut(s) 135, 585, 596, 867
BfuAI ACCTGC 1 cut(s) 635
BfuI GTATCC 3 cut(s) 32, 659, 2212
BisI GCNGC 7 cut(s) 1332, 1571, 2144, 2169, 2859, 2862, 2865
BlnI CCTAGG 1 cut(s) 1310
BlsI GCNGC 7 cut(s) 1333, 1572, 2145, 2170, 2860, 2863, 2866
BmcAI AGTACT 1 cut(s) 1756
Bme1390I CCNGG 6 cut(s) 710, 1260, 1323, 1548, 1683, 2206
Bme18I GGWCC 1 cut(s) 1955
BmeRI GACNNNNNGTC 1 cut(s) 2368
BmgBI CACGTC 1 cut(s) 1997
BmgT120I GGNCC 2 cut(s) 300, 1955
BmiI GGNNCC 7 cut(s) 147, 1230, 1462, 1768, 1888, 2086, 2307
BmrFI CCNGG 6 cut(s) 710, 1260, 1323, 1548, 1683, 2206
BmrI ACTGGG 5 cut(s) 20, 365, 431, 1067, 2485
BmsI GCATC 3 cut(s) 2018, 2679, 2860
BmuI ACTGGG 5 cut(s) 20, 365, 431, 1067, 2485
BpiI GAAGAC 1 cut(s) 1581
BplI GAGNNNNNCTC 4 cut(s) 1525, 1557, 1975, 2007
BpmI CTGGAG 2 cut(s) 897, 1989
Bpu10I CCTNAGC 4 cut(s) 1233, 1698, 1803, 1819
Bpu14I TTCGAA 1 cut(s) 1286
BpuEI CTTGAG 2 cut(s) 1631, 2605
BpuMI CCSGG 4 cut(s) 710, 1548, 1683, 2206
BsaJI CCNNGG 8 cut(s) 669, 1212, 1258, 1310, 1770, 2159, 2264, 2808
BsaWI WCCGGW 2 cut(s) 1093, 1628
BsaXI ACNNNNNCTCC 4 cut(s) 377, 407, 955, 985
Bsc4I CCNNNNNNNGG 4 cut(s) 319, 385, 872, 1322
Bse21I CCTNAGG 1 cut(s) 1005
Bse3DI GCAATG 3 cut(s) 639, 2304, 2410
BseAI TCCGGA 1 cut(s) 1093
BseBI CCWGG 2 cut(s) 1260, 1323
BseDI CCNNGG 8 cut(s) 669, 1212, 1258, 1310, 1770, 2159, 2264, 2808
BseGI GGATG 8 cut(s) 286, 679, 842, 1814, 1895, 2027, 2036, 2364
BseLI CCNNNNNNNGG 4 cut(s) 319, 385, 872, 1322
BseMI GCAATG 3 cut(s) 639, 2304, 2410
BseRI GAGGAG 1 cut(s) 2148
BseSI GKGCMC 1 cut(s) 2089
BseXI GCAGC 5 cut(s) 1318, 1557, 2155, 2155, 2873
BseYI CCCAGC 1 cut(s) 872
BsgI GTGCAG 1 cut(s) 2162
Bsh1236I CGCG 1 cut(s) 169
BshFI GGCC 5 cut(s) 302, 725, 1315, 2768, 2807
BshNI GGYRCC 2 cut(s) 1766, 2084
BsiHKAI GWGCWC 1 cut(s) 545
BsiSI CCGG 6 cut(s) 710, 1094, 1547, 1629, 1683, 2206
BslI CCNNNNNNNGG 4 cut(s) 319, 385, 872, 1322
BsmAI GTCTC 5 cut(s) 1605, 2263, 2695, 2756, 2819
BsmBI CGTCTC 1 cut(s) 2756
BsmI GAATGC 1 cut(s) 130
BsnI GGCC 5 cut(s) 302, 725, 1315, 2768, 2807
Bsp119I TTCGAA 1 cut(s) 1286
Bsp1286I GDGCHC 2 cut(s) 545, 2089
Bsp13I TCCGGA 1 cut(s) 1093
Bsp19I CCATGG 2 cut(s) 1212, 1770
BspACI CCGC 3 cut(s) 503, 2858, 2864
BspANI GGCC 5 cut(s) 302, 725, 1315, 2768, 2807
BspEI TCCGGA 1 cut(s) 1093
BspFNI CGCG 1 cut(s) 169
BspHI TCATGA 1 cut(s) 1081
BspLI GGNNCC 7 cut(s) 147, 1230, 1462, 1768, 1888, 2086, 2307
BspMI ACCTGC 1 cut(s) 635
BspQI GCTCTTC 2 cut(s) 1422, 2621
BspT104I TTCGAA 1 cut(s) 1286
BspT107I GGYRCC 2 cut(s) 1766, 2084
BsrDI GCAATG 3 cut(s) 639, 2304, 2410
BssECI CCNNGG 8 cut(s) 669, 1212, 1258, 1310, 1770, 2159, 2264, 2808
BssT1I CCWWGG 5 cut(s) 1212, 1310, 1770, 2264, 2808
Bst2UI CCWGG 2 cut(s) 1260, 1323
Bst4CI ACNGT 9 cut(s) 589, 1171, 1303, 1490, 2198, 2315, 2433, 2458, 2830
Bst6I CTCTTC 6 cut(s) 1005, 1422, 2621, 2754, 2786, 2844
BstBI TTCGAA 1 cut(s) 1286
BstC8I GCNNGC 3 cut(s) 1860, 2770, 2774
BstDSI CCRYGG 2 cut(s) 1212, 1770
BstENI CCTNNNNNAGG 1 cut(s) 1320
BstF5I GGATG 8 cut(s) 286, 679, 842, 1814, 1895, 2027, 2036, 2364
BstFNI CGCG 1 cut(s) 169
BstMAI GTCTC 5 cut(s) 1605, 2263, 2695, 2756, 2819
BstMWI GCNNNNNNNGC 5 cut(s) 731, 1048, 2676, 2864, 2870
BstNI CCWGG 2 cut(s) 1260, 1323
BstNSI RCATGY 3 cut(s) 1580, 1862, 2877
BstSCI CCNGG 6 cut(s) 708, 1258, 1321, 1546, 1681, 2204
BstSFI CTRYAG 4 cut(s) 135, 585, 596, 867
BstSLI GKGCMC 1 cut(s) 2089
BstUI CGCG 1 cut(s) 169
BstV1I GCAGC 5 cut(s) 1318, 1557, 2155, 2155, 2873
BstV2I GAAGAC 1 cut(s) 1581
BstX2I RGATCY 3 cut(s) 559, 797, 1886
BstXI CCANNNNNNTGG 4 cut(s) 281, 1219, 2375, 2498
BstYI RGATCY 3 cut(s) 559, 797, 1886
Bsu36I CCTNAGG 1 cut(s) 1005
BsuI GTATCC 3 cut(s) 32, 659, 2212
BsuRI GGCC 5 cut(s) 302, 725, 1315, 2768, 2807
BtgI CCRYGG 2 cut(s) 1212, 1770
BtrI CACGTC 1 cut(s) 1997
BtsCI GGATG 8 cut(s) 286, 679, 842, 1814, 1895, 2027, 2036, 2364
BtsI GCAGTG 1 cut(s) 1504
BveI ACCTGC 1 cut(s) 635
Cac8I GCNNGC 3 cut(s) 1860, 2770, 2774
CaiI CAGNNNCTG 1 cut(s) 1328
CciI TCATGA 1 cut(s) 1081
Cfr13I GGNCC 2 cut(s) 300, 1955
Csp6I GTAC 3 cut(s) 1755, 1767, 2001
CviAII CATG 9 cut(s) 806, 1082, 1213, 1577, 1771, 1859, 2041, 2099, 2874
CviQI GTAC 3 cut(s) 1755, 1767, 2001
DriI GACNNNNNGTC 1 cut(s) 2368
EaeI YGGCCR 1 cut(s) 2766
Eam1104I CTCTTC 6 cut(s) 1005, 1422, 2621, 2754, 2786, 2844
Eam1105I GACNNNNNGTC 1 cut(s) 2368
EarI CTCTTC 6 cut(s) 1005, 1422, 2621, 2754, 2786, 2844
Ecl136II GAGCTC 1 cut(s) 543
Eco130I CCWWGG 5 cut(s) 1212, 1310, 1770, 2264, 2808
Eco147I AGGCCT 2 cut(s) 1315, 2807
Eco24I GRGCYC 1 cut(s) 545
Eco32I GATATC 4 cut(s) 178, 532, 888, 1296
Eco47I GGWCC 1 cut(s) 1955
Eco53kI GAGCTC 1 cut(s) 543
Eco57I CTGAAG 2 cut(s) 2228, 2825
Eco81I CCTNAGG 1 cut(s) 1005
EcoICRI GAGCTC 1 cut(s) 543
EcoNI CCTNNNNNAGG 1 cut(s) 1320
EcoO109I RGGNCCY 1 cut(s) 300
EcoRI GAATTC 1 cut(s) 634
EcoRII CCWGG 2 cut(s) 1258, 1321
EcoRV GATATC 4 cut(s) 178, 532, 888, 1296
EcoT14I CCWWGG 5 cut(s) 1212, 1310, 1770, 2264, 2808
EcoT22I ATGCAT 1 cut(s) 2875
EcoT38I GRGCYC 1 cut(s) 545
ErhI CCWWGG 5 cut(s) 1212, 1310, 1770, 2264, 2808
Esp3I CGTCTC 1 cut(s) 2756
FaeI CATG 9 cut(s) 809, 1085, 1216, 1580, 1774, 1862, 2044, 2102, 2877
FalI AAGNNNNNCTT 2 cut(s) 1898, 1930
FatI CATG 9 cut(s) 805, 1081, 1212, 1576, 1770, 1858, 2040, 2098, 2873
FbaI TGATCA 1 cut(s) 2731
Fnu4HI GCNGC 7 cut(s) 1332, 1571, 2144, 2169, 2859, 2862, 2865
FokI GGATG 8 cut(s) 293, 686, 849, 1801, 1882, 2014, 2023, 2351
FriOI GRGCYC 1 cut(s) 545
Fsp4HI GCNGC 7 cut(s) 1332, 1571, 2144, 2169, 2859, 2862, 2865
FspBI CTAG 3 cut(s) 759, 1311, 2787
GluI GCNGC 7 cut(s) 1332, 1571, 2144, 2169, 2859, 2862, 2865
GsaI CCCAGC 1 cut(s) 876
GsuI CTGGAG 2 cut(s) 897, 1989
HaeIII GGCC 5 cut(s) 302, 725, 1315, 2768, 2807
HapII CCGG 6 cut(s) 710, 1094, 1547, 1629, 1683, 2206
Hin1II CATG 9 cut(s) 809, 1085, 1216, 1580, 1774, 1862, 2044, 2102, 2877
HincII GTYRAC 2 cut(s) 1656, 2373
HindII GTYRAC 2 cut(s) 1656, 2373
HindIII AAGCTT 2 cut(s) 1849, 2774
HpaII CCGG 6 cut(s) 710, 1094, 1547, 1629, 1683, 2206
Hpy166II GTNNAC 4 cut(s) 165, 1439, 1656, 2373
Hpy8I GTNNAC 4 cut(s) 165, 1439, 1656, 2373
Hpy99I CGWCG 1 cut(s) 1998
HpyCH4III ACNGT 9 cut(s) 589, 1171, 1303, 1490, 2198, 2315, 2433, 2458, 2830
HpyCH4IV ACGT 3 cut(s) 1441, 1921, 1996
HpyCH4V TGCA 7 cut(s) 644, 816, 1573, 1862, 2143, 2171, 2873
HpyF10VI GCNNNNNNNGC 5 cut(s) 731, 1048, 2676, 2864, 2870
HpySE526I ACGT 3 cut(s) 1441, 1921, 1996
Hsp92II CATG 9 cut(s) 809, 1085, 1216, 1580, 1774, 1862, 2044, 2102, 2877
Kpn2I TCCGGA 1 cut(s) 1093
KpnI GGTACC 1 cut(s) 1770
Ksp22I TGATCA 1 cut(s) 2731
LguI GCTCTTC 2 cut(s) 1422, 2621
LmnI GCTCC 3 cut(s) 457, 540, 1934
Lsp1109I GCAGC 5 cut(s) 1318, 1557, 2155, 2155, 2873
LweI GCATC 3 cut(s) 2018, 2679, 2860
MaeI CTAG 3 cut(s) 759, 1311, 2787
MaeII ACGT 3 cut(s) 1441, 1921, 1996
MfeI CAATTG 1 cut(s) 2172
MflI RGATCY 3 cut(s) 559, 797, 1886
MhlI GDGCHC 2 cut(s) 545, 2089
MluI ACGCGT 1 cut(s) 167
MmeI TCCRAC 2 cut(s) 229, 258
Mph1103I ATGCAT 1 cut(s) 2875
MroI TCCGGA 1 cut(s) 1093
MroXI GAANNNNTTC 5 cut(s) 130, 634, 967, 1171, 2741
MseI TTAA 4 cut(s) 1116, 1403, 1929, 2633
MslI CAYNNNNRTG 2 cut(s) 947, 2709
MspA1I CMGCKG 3 cut(s) 1823, 2858, 2864
MspI CCGG 6 cut(s) 710, 1094, 1547, 1629, 1683, 2206
MspR9I CCNGG 6 cut(s) 710, 1260, 1323, 1548, 1683, 2206
MunI CAATTG 1 cut(s) 2172
Mva1269I GAATGC 1 cut(s) 130
MvaI CCWGG 2 cut(s) 1260, 1323
MvnI CGCG 1 cut(s) 169
MwoI GCNNNNNNNGC 5 cut(s) 731, 1048, 2676, 2864, 2870
NciI CCSGG 4 cut(s) 710, 1548, 1683, 2206
NcoI CCATGG 2 cut(s) 1212, 1770
NlaIII CATG 9 cut(s) 809, 1085, 1216, 1580, 1774, 1862, 2044, 2102, 2877
NlaIV GGNNCC 7 cut(s) 147, 1230, 1462, 1768, 1888, 2086, 2307
NmuCI GTSAC 8 cut(s) 56, 116, 496, 677, 925, 949, 1129, 1153
NsiI ATGCAT 1 cut(s) 2875
NspI RCATGY 3 cut(s) 1580, 1862, 2877
NspV TTCGAA 1 cut(s) 1286
PaeI GCATGC 1 cut(s) 1862
PagI TCATGA 1 cut(s) 1081
PceI AGGCCT 2 cut(s) 1315, 2807
PciI ACATGT 1 cut(s) 1576
PciSI GCTCTTC 2 cut(s) 1422, 2621
PctI GAATGC 1 cut(s) 130
PdmI GAANNNNTTC 5 cut(s) 130, 634, 967, 1171, 2741
PfeI GAWTC 8 cut(s) 88, 917, 1097, 1208, 1748, 1775, 1940, 2520
PfoI TCCNGGA 2 cut(s) 1681, 2204
PkrI GCNGC 7 cut(s) 1333, 1572, 2145, 2170, 2860, 2863, 2866
PscI ACATGT 1 cut(s) 1576
PsiI TTATAA 1 cut(s) 1641
Psp124BI GAGCTC 1 cut(s) 545
Psp6I CCWGG 2 cut(s) 1258, 1321
PspFI CCCAGC 1 cut(s) 872
PspGI CCWGG 2 cut(s) 1258, 1321
PspN4I GGNNCC 7 cut(s) 147, 1230, 1462, 1768, 1888, 2086, 2307
PspPI GGNCC 2 cut(s) 300, 1955
PstNI CAGNNNCTG 1 cut(s) 1328
PsuI RGATCY 3 cut(s) 559, 797, 1886
PvuII CAGCTG 1 cut(s) 1823
RsaI GTAC 3 cut(s) 1756, 1768, 2002
RsaNI GTAC 3 cut(s) 1755, 1767, 2001
RseI CAYNNNNRTG 2 cut(s) 947, 2709
SacI GAGCTC 1 cut(s) 545
SapI GCTCTTC 2 cut(s) 1422, 2621
SaqAI TTAA 4 cut(s) 1116, 1403, 1929, 2633
SatI GCNGC 7 cut(s) 1332, 1571, 2144, 2169, 2859, 2862, 2865
Sau96I GGNCC 2 cut(s) 300, 1955
ScaI AGTACT 1 cut(s) 1756
ScrFI CCNGG 6 cut(s) 710, 1260, 1323, 1548, 1683, 2206
SduI GDGCHC 2 cut(s) 545, 2089
SfaNI GCATC 3 cut(s) 2018, 2679, 2860
SfcI CTRYAG 4 cut(s) 135, 585, 596, 867
SfuI TTCGAA 1 cut(s) 1286
SinI GGWCC 1 cut(s) 1955
SmiMI CAYNNNNRTG 2 cut(s) 947, 2709
SmlI CTYRAG 2 cut(s) 1646, 2620
SmoI CTYRAG 2 cut(s) 1646, 2620
SphI GCATGC 1 cut(s) 1862
SseBI AGGCCT 2 cut(s) 1315, 2807
SsiI CCGC 3 cut(s) 503, 2858, 2864
SspMI CTAG 3 cut(s) 759, 1311, 2787
SstI GAGCTC 1 cut(s) 545
StuI AGGCCT 2 cut(s) 1315, 2807
StyD4I CCNGG 6 cut(s) 708, 1258, 1321, 1546, 1681, 2204
StyI CCWWGG 5 cut(s) 1212, 1310, 1770, 2264, 2808
TaaI ACNGT 9 cut(s) 589, 1171, 1303, 1490, 2198, 2315, 2433, 2458, 2830
TaiI ACGT 3 cut(s) 1444, 1924, 1999
TaqI TCGA 5 cut(s) 1286, 1945, 1993, 2135, 2781
TatI WGTACW 1 cut(s) 1754
TauI GCSGC 2 cut(s) 2861, 2867
TfiI GAWTC 8 cut(s) 88, 917, 1097, 1208, 1748, 1775, 1940, 2520
Tru1I TTAA 4 cut(s) 1116, 1403, 1929, 2633
Tru9I TTAA 4 cut(s) 1116, 1403, 1929, 2633
TseFI GTSAC 8 cut(s) 56, 116, 496, 677, 925, 949, 1129, 1153
TseI GCWGC 5 cut(s) 1331, 1570, 2143, 2168, 2861
Tsp45I GTSAC 8 cut(s) 56, 116, 496, 677, 925, 949, 1129, 1153
TspDTI ATGAA 4 cut(s) 246, 1010, 1404, 2043
VpaK11BI GGWCC 1 cut(s) 1955
XagI CCTNNNNNAGG 1 cut(s) 1320
XapI RAATTY 5 cut(s) 634, 850, 1282, 1410, 2276
XceI RCATGY 3 cut(s) 1580, 1862, 2877
XmaJI CCTAGG 1 cut(s) 1310
XmnI GAANNNNTTC 5 cut(s) 130, 634, 967, 1171, 2741
XspI CTAG 3 cut(s) 759, 1311, 2787
ZrmI AGTACT 1 cut(s) 1756
Zsp2I ATGCAT 1 cut(s) 2875
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.