pycom06g17610

Plant calmodulin-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0012620
Physical Location & Seq
Forward (+)
21535236 .. 21538472
3237 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g17610.1

Sequence Viewer

Length: 3237 bp
ATGGAAGCAGAGAACATCGGTATCCCAGTGATCCTACAAGGCCTGGACCTTCTTGGTGAAAAGGCCAGAAAGAATTCGATCCCACAGGAAGGGGAGTCCAATGGTGGTGATGTTGGCAGTGATATCAGAAGGGATTCTATCACGGAGATCCCACCGGAAATGGATCCCAAGGGTGGTGATGTCAGGGGTAACTCTGATCCAGGGGTTTCACAGGACATAGAGCCCAATGGCGGGGATAGCAGAAGGAGCTCTATCCCAGTGATCCCACAGGAGATGGATCCCAAGGGTGGTGATGTCAGAGGTAATCCGGTCATGGGGATTCCACAGGACATAGAGCCCAATAGAGGTGACATCAGAAGGAGCTCTATCGGAAATCTTGATCCTCCTTCTACCGAGAGGAACTCTATCCCAGTGGTCCTGCAGGACATGGATCTCAGTGATGATGATAGGAACTTCTTTGCAGCTGTCCCACAGGATATAGAACCCGATGGCAGTGATATCAAACAGAAATCTATCCTAGCAATCCCACAGAAAATGGAGCATGAGGGTGGTGATATCGGAAGGAAGCCTATCCTAGTGAGGCCACAGGAAATAGAGTCTAATGCCGGTGATATCAGAAGAAACTCCATAGAGAAAGTAGATCCTCCTGCTGATTTCAAAAGGAACTCTATCCCAGTGATCCCACAGGAAATGAAGCCTAATGCTGGCGATGTTAGAAGGAACTCTATTGGTGTGATCCCACAGGAATTAGAGCACAGTGGCAGTGGTATCAGAAGGAACTCTATGCCAGAGGTCCCACAGAAAATGGAACCAAAGGCTGGCGATACCAGCAGGAACTCTGTTCTGTGGATCCCGCATGAAGTGGAACCAAAGGGTGGTAGATGGATTCAGTCCCGATATCTTAGAGCTCCGATAAGTTCCTGCCATGATTATTGCAAATATGGAGTGAGAAATGCCTCTGAAGAAAATGCAACTGCTGCACTGCGAAAATCAGCTACAGAGAGGAAACGGATATCCACAACCAGTCAAAAGCCTTCTCCAGATTCAGACACCCAGAAGCTTGACAGTTCTGTCGTCACAGAGAAAAAAGTATTGTCATTGACAAAGAAAGAAATTGTTTCTGCAGAAAAAGTTCCATCTTCTTCCAAGGACATTGATGTTTCTATGGAAGATTCCGCTGGTTTGAAGGTGAAACGTGTGCAATCAGAGCCATCTTCTCTCCCAGAAGCGGCCTTAAGCCTTAGAAATTCAAAGGTGATATCCACAACTGGACCGAGGTCTGTTCCACATTCCCAAACCCGGAAACTTAATAATTATGTTGTCACGGAGAAGAAAGGTTCATCGTGGACGAAAGAAACTTCTTCAAAGAAAGTTTCATCTCCCAAGGAAATAGCTGTTTGTATGGAAGAGTTGATTGATTCAAAGATGAAGGATAAGCAATTAGATCAATCTTCTCTCCCAGGATCAGACCCAAGCTTGAGAAATTTGGAGGTTACATCAACAATCAATCCTAACCCTTCTCCAGATTCTGAGTCCCAGAGTTCTACTGCCACGGCGAAAAGAGTTTCACCTCCCTATAAGGATATAGATGTTTCTGCTGAAGATTCCACTGATTCAAAGGTGATATCAACAGTCAGTCCTAATCCTTCTCCAGATTCTGAAGCACAGGAACTTAATCATTCTGGTGGCACAGACAAAAGAGTTTTATCATCGAAAAGGAAAGAAAATGTCTCTTCAAAAACAGTTTTGACTCCCATCAAGGAGATTGATGTTTCTACGGAAGGTGACGCCTGTTCAAAGGTGAAGCCTGAGCAACTAGAGCCATCTTCTCGCACAGGACAAGGTTCAAGTAATCAAGGAAAGAGTGGAACTAGAAAGGGCAAGGAAATAAAAGAAGGTTCGGGCAGTAGTGTAAATAGAAAAATCAGAAGCAAGCTGCAAAGGACTCCTGTATTGGTTGAGAAAAAGATGTTAGAGCCTGAAACTGTTTGTTTGACTTCCAAACACCCTGTCAAGAGAGTCTCCGATGCAAATGCTGGAAGCCCTAAGAACCTTAAAGGGCTGTCTCATATGAAAGATCAAAAGTGTCCCCGAAAAGTTGAACCTGAGGGAACCTGCAATAAGGATGTTCCGGAAAAGATTTTGTATGTCGTCGAATCTAATACTGAGAATAGTACTGTCAAACCAACTCCAAATGGTGCCGATGCCCCTGAGTTATCATCATCATCACATCTATCCTCTGATGTCAAAAGCTTGAGGCAAGCAAGGAAGGGAGTTGGTACCACTCGATCACCTACATCCTCAAAGAAGAAAAACTTCAAACGCACTGTAAATGGAGGTTATGGTCGTTCACCTACTGCTCCACCCCCTGAAAACAAAGGTTTGAGGCGGAGTACTCGAAGGCCTTTATCTTCATCGTTGTCGTCATCATCATCATCATCCACGTCCGCATCCCCAGCCCAGTCCACTCATAAAAGAGAAAATGGCGCCACGTCTCAACACAGAGCAATGAAAACTGGTAATCAAAGAGAAAATTTGAAGGTGGAGAAGAGTACTAGGCCTCGAAAGTCTGAGACACATCGCTCTGAAAGTAAAAACAGCTCAGCCCGAAAGCTGACATTTAAGAGAGGAAGGGTGCTTGACATAAAGCCTGAGATCAGTACTACAAGGAGACTCAAATTCAGGCGAGTAAGATTGGCAGGGGAGATCCAAAATGGTAAAGGTGGTGTCACAGGGGGGAGCGCCGGGAGGAAAGAAGTTTGTGACAACCGTTCAGTTGGTGATGTATTAAAACAGTTGAGCCCCGGGAGGAAAGTAGCTGATCACAGTCACTCAAATGGTGCTTTCATCAAAAGGCATAGCCCCGGGACGAAAGAAGTTGATGGCAGCCAGTCAAATGGTGGTAAATTCAAAAGTGAGAAAGTTGTTCTGAGAAGCTTCGAACGAGATGTGGAAGGTTCACGAAGGAAGAGCTTGAGGAGGAAAACGGCTAGTGATAGCGGGTTGAATGGTAGTACTAGAACGGAACCTGAGAGGGTTGTGTTGAGACATCAAGATGTGAAAGGAAGGAAGGATGTGCAGAAGTTGTTAAATAATGTGATCGAAGAGACTGCCAGTAGGCTTGTTGTGTCCAGGAAGAGTAAAGTCAAGGCCTTGGTTGGTGCTTTTGAGACCGTGATTTCGCGTCAGGATACCAAATCCATGGCTTCGGAAACAGACACGGAAGATGCAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaM_binding

Protein Analysis

1079

Amino Acids

117.29

Weight (kDa)

9.65

Isoelectric Point (pI)

64.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CaM_binding PF07839 966 - 1061 2.2e-26 Plant calmodulin-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1070
Acc36I ACCTGC 1 cut(s) 2123
Acc65I GGTACC 1 cut(s) 2279
AccB1I GGYRCC 3 cut(s) 2198, 2279, 2486
AccB7I CCANNNNNTGG 2 cut(s) 818, 875
AccII CGCG 1 cut(s) 3184
AccIII TCCGGA 1 cut(s) 2131
AciI CCGC 7 cut(s) 231, 854, 1176, 1229, 2389, 2448, 3000
AcsI RAATTY 6 cut(s) 73, 1246, 1483, 2533, 2678, 2906
AcuI CTGAAG 3 cut(s) 981, 1620, 1680
AcyI GRCGYC 2 cut(s) 1788, 2487
AfaI GTAC 6 cut(s) 2176, 2281, 2395, 2554, 2662, 3016
AflII CTTAAG 1 cut(s) 1234
AflIII ACRYGT 1 cut(s) 1195
AjiI CACGTC 2 cut(s) 2445, 2493
AjnI CCWGG 4 cut(s) 42, 199, 1459, 3131
Alw21I GWGCWC 4 cut(s) 251, 365, 756, 910
Alw26I GTCTC 9 cut(s) 1735, 2026, 2070, 2499, 2567, 2665, 3040, 3101, 3164
AlwNI CAGNNNCTG 2 cut(s) 1529, 1658
Ama87I CYCGRG 2 cut(s) 2804, 2864
Aor13HI TCCGGA 1 cut(s) 2131
AoxI GGCC 7 cut(s) 40, 63, 581, 1230, 2402, 2558, 3150
ApeKI GCWGC 4 cut(s) 461, 977, 1936, 2886
ApoI RAATTY 6 cut(s) 73, 1246, 1483, 2533, 2678, 2906
ArsI GACNNNNNNTTYG 4 cut(s) 1995, 2027, 3163, 3195
Asp700I GAANNNNTTC 4 cut(s) 73, 133, 1131, 2315
Asp718I GGTACC 1 cut(s) 2279
AspLEI GCGC 2 cut(s) 2489, 2744
AspS9I GGNCC 4 cut(s) 46, 415, 793, 1271
AsuC2I CCSGG 6 cut(s) 1300, 2746, 2805, 2806, 2865, 2866
AsuII TTCGAA 1 cut(s) 2940
AvaI CYCGRG 2 cut(s) 2804, 2864
AvaII GGWCC 4 cut(s) 46, 415, 793, 1271
AxyI CCTNAGG 1 cut(s) 2106
BaeI ACNNNNGTAYC 1 cut(s) 37
BamHI GGATCC 3 cut(s) 163, 277, 849
BanI GGYRCC 3 cut(s) 2198, 2279, 2486
BanII GRGCYC 6 cut(s) 225, 251, 339, 365, 910, 2804
Bbv12I GWGCWC 4 cut(s) 251, 365, 756, 910
BbvI GCAGC 4 cut(s) 473, 964, 1923, 2898
BccI CCATC 8 cut(s) 268, 482, 876, 1144, 1219, 1763, 1831, 2876
BceAI ACGGC 2 cut(s) 1569, 3003
BcgI CGANNNNNNTGC 4 cut(s) 2015, 2049, 3092, 3126
BciT130I CCWGG 4 cut(s) 44, 201, 1461, 3133
BciVI GTATCC 2 cut(s) 32, 3184
BclI TGATCA 1 cut(s) 2821
BcnI CCSGG 6 cut(s) 1300, 2746, 2805, 2806, 2865, 2866
BcoDI GTCTC 9 cut(s) 1735, 2026, 2070, 2499, 2567, 2665, 3040, 3101, 3164
BfaI CTAG 7 cut(s) 518, 575, 1817, 1872, 2556, 2991, 3018
BfmI CTRYAG 3 cut(s) 419, 996, 1122
BfoI RGCGCY 2 cut(s) 2490, 2745
BfrI CTTAAG 1 cut(s) 1234
BfuAI ACCTGC 1 cut(s) 2123
BfuI GTATCC 2 cut(s) 32, 3184
BisI GCNGC 5 cut(s) 462, 978, 1230, 1937, 2887
BlpI GCTNAGC 1 cut(s) 2602
BlsI GCNGC 5 cut(s) 463, 979, 1231, 1938, 2888
BmcAI AGTACT 5 cut(s) 2176, 2395, 2554, 2662, 3016
Bme18I GGWCC 4 cut(s) 46, 415, 793, 1271
BmeT110I CYCGRG 2 cut(s) 2804, 2864
BmgBI CACGTC 2 cut(s) 2445, 2493
BmgT120I GGNCC 4 cut(s) 46, 415, 793, 1271
BmrI ACTGGG 5 cut(s) 20, 251, 404, 668, 2455
BmsI GCATC 4 cut(s) 2017, 2194, 2459, 3217
BmuI ACTGGG 5 cut(s) 20, 251, 404, 668, 2455
BoxI GACNNNNGTC 1 cut(s) 1276
BplI GAGNNNNNCTC 2 cut(s) 386, 418
BpmI CTGGAG 3 cut(s) 1023, 1506, 1635
Bpu10I CCTNAGC 1 cut(s) 1809
Bpu1102I GCTNAGC 1 cut(s) 2602
Bpu14I TTCGAA 1 cut(s) 2940
BpuEI CTTGAG 3 cut(s) 1498, 2275, 2995
BpuMI CCSGG 6 cut(s) 1300, 2746, 2805, 2806, 2865, 2866
BsaHI GRCGYC 2 cut(s) 1788, 2487
BsaI GGTCTC 1 cut(s) 3164
BsaWI WCCGGW 3 cut(s) 154, 307, 2131
Bse118I RCCGGY 1 cut(s) 605
Bse21I CCTNAGG 1 cut(s) 2106
Bse3DI GCAATG 1 cut(s) 2514
BseAI TCCGGA 1 cut(s) 2131
BseBI CCWGG 4 cut(s) 44, 201, 1461, 3133
BseGI GGATG 5 cut(s) 2131, 2297, 2438, 2450, 3079
BseMI GCAATG 1 cut(s) 2514
BseRI GAGGAG 1 cut(s) 2992
BseXI GCAGC 4 cut(s) 473, 964, 1923, 2898
BseYI CCCAGC 1 cut(s) 2455
BsgI GTGCAG 2 cut(s) 963, 3098
Bsh1236I CGCG 1 cut(s) 3184
BshFI GGCC 7 cut(s) 42, 65, 583, 1232, 2404, 2560, 3152
BshNI GGYRCC 3 cut(s) 2198, 2279, 2486
BsiHKAI GWGCWC 4 cut(s) 251, 365, 756, 910
BsiHKCI CYCGRG 2 cut(s) 2804, 2864
BsiSI CCGG 8 cut(s) 155, 308, 606, 1300, 2132, 2745, 2805, 2865
BslFI GGGAC 6 cut(s) 452, 779, 877, 1519, 2073, 2881
BsmAI GTCTC 9 cut(s) 1735, 2026, 2070, 2499, 2567, 2665, 3040, 3101, 3164
BsmBI CGTCTC 1 cut(s) 2499
BsmFI GGGAC 6 cut(s) 452, 779, 877, 1519, 2073, 2881
BsnI GGCC 7 cut(s) 42, 65, 583, 1232, 2404, 2560, 3152
Bso31I GGTCTC 1 cut(s) 3164
BsoBI CYCGRG 2 cut(s) 2804, 2864
Bsp119I TTCGAA 1 cut(s) 2940
Bsp1286I GDGCHC 7 cut(s) 225, 251, 339, 365, 756, 910, 2804
Bsp13I TCCGGA 1 cut(s) 2131
Bsp1720I GCTNAGC 1 cut(s) 2602
Bsp19I CCATGG 1 cut(s) 3201
BspACI CCGC 7 cut(s) 231, 854, 1176, 1229, 2389, 2448, 3000
BspANI GGCC 7 cut(s) 42, 65, 583, 1232, 2404, 2560, 3152
BspEI TCCGGA 1 cut(s) 2131
BspFNI CGCG 1 cut(s) 3184
BspMAI CTGCAG 2 cut(s) 423, 1126
BspMI ACCTGC 1 cut(s) 2123
BspQI GCTCTTC 1 cut(s) 2963
BspT104I TTCGAA 1 cut(s) 2940
BspT107I GGYRCC 3 cut(s) 2198, 2279, 2486
BspTI CTTAAG 1 cut(s) 1234
BspTNI GGTCTC 1 cut(s) 3164
BsrDI GCAATG 1 cut(s) 2514
BsrFI RCCGGY 1 cut(s) 605
BssAI RCCGGY 1 cut(s) 605
BssNI GRCGYC 2 cut(s) 1788, 2487
BssT1I CCWWGG 6 cut(s) 168, 282, 1146, 1383, 3153, 3201
Bst2UI CCWGG 4 cut(s) 44, 201, 1461, 3133
Bst6I CTCTTC 6 cut(s) 1401, 1738, 2543, 2963, 3099, 3131
BstACI GRCGYC 2 cut(s) 1788, 2487
BstAFI CTTAAG 1 cut(s) 1234
BstAPI GCANNNNNTGC 1 cut(s) 977
BstBI TTCGAA 1 cut(s) 2940
BstC8I GCNNGC 4 cut(s) 706, 820, 1934, 2262
BstDSI CCRYGG 2 cut(s) 1551, 3201
BstF5I GGATG 5 cut(s) 2131, 2297, 2438, 2450, 3079
BstFNI CGCG 1 cut(s) 3184
BstH2I RGCGCY 2 cut(s) 2490, 2745
BstHHI GCGC 2 cut(s) 2489, 2744
BstMAI GTCTC 9 cut(s) 1735, 2026, 2070, 2499, 2567, 2665, 3040, 3101, 3164
BstMWI GCNNNNNNNGC 7 cut(s) 237, 246, 828, 977, 1207, 1819, 2456
BstNI CCWGG 4 cut(s) 44, 201, 1461, 3133
BstPAI GACNNNNGTC 1 cut(s) 1276
BstSFI CTRYAG 3 cut(s) 419, 996, 1122
BstUI CGCG 1 cut(s) 3184
BstV1I GCAGC 4 cut(s) 473, 964, 1923, 2898
BstX2I RGATCY 7 cut(s) 147, 163, 277, 430, 640, 849, 2706
BstXI CCANNNNNNTGG 2 cut(s) 2897, 3202
BstYI RGATCY 7 cut(s) 147, 163, 277, 430, 640, 849, 2706
Bsu36I CCTNAGG 1 cut(s) 2106
BsuI GTATCC 2 cut(s) 32, 3184
BsuRI GGCC 7 cut(s) 42, 65, 583, 1232, 2404, 2560, 3152
BtgI CCRYGG 2 cut(s) 1551, 3201
BtgZI GCGATG 2 cut(s) 723, 2564
BtrI CACGTC 2 cut(s) 2445, 2493
BtsCI GGATG 5 cut(s) 2131, 2297, 2438, 2450, 3079
BtsI GCAGTG 4 cut(s) 124, 499, 769, 980
BveI ACCTGC 1 cut(s) 2123
Cac8I GCNNGC 4 cut(s) 706, 820, 1934, 2262
CaiI CAGNNNCTG 2 cut(s) 1529, 1658
CfoI GCGC 2 cut(s) 2489, 2744
Cfr10I RCCGGY 1 cut(s) 605
Cfr13I GGNCC 4 cut(s) 46, 415, 793, 1271
Cfr9I CCCGGG 2 cut(s) 2804, 2864
CseI GACGC 2 cut(s) 1796, 3173
Csp6I GTAC 6 cut(s) 2175, 2280, 2394, 2553, 2661, 3015
CviAII CATG 6 cut(s) 313, 427, 542, 857, 926, 3202
CviQI GTAC 6 cut(s) 2175, 2280, 2394, 2553, 2661, 3015
DinI GGCGCC 1 cut(s) 2488
DrdI GACNNNNNNGTC 1 cut(s) 1070
DseDI GACNNNNNNGTC 1 cut(s) 1070
Eam1104I CTCTTC 6 cut(s) 1401, 1738, 2543, 2963, 3099, 3131
EarI CTCTTC 6 cut(s) 1401, 1738, 2543, 2963, 3099, 3131
EciI GGCGGA 1 cut(s) 2404
Ecl136II GAGCTC 3 cut(s) 249, 363, 908
Eco130I CCWWGG 6 cut(s) 168, 282, 1146, 1383, 3153, 3201
Eco147I AGGCCT 4 cut(s) 42, 2404, 2560, 3152
Eco24I GRGCYC 6 cut(s) 225, 251, 339, 365, 910, 2804
Eco31I GGTCTC 1 cut(s) 3164
Eco32I GATATC 8 cut(s) 124, 499, 556, 613, 899, 1014, 1260, 1626
Eco47I GGWCC 4 cut(s) 46, 415, 793, 1271
Eco53kI GAGCTC 3 cut(s) 249, 363, 908
Eco57I CTGAAG 3 cut(s) 981, 1620, 1680
Eco81I CCTNAGG 1 cut(s) 2106
Eco88I CYCGRG 2 cut(s) 2804, 2864
EcoICRI GAGCTC 3 cut(s) 249, 363, 908
EcoO109I RGGNCCY 1 cut(s) 793
EcoRI GAATTC 1 cut(s) 73
EcoRII CCWGG 4 cut(s) 42, 199, 1459, 3131
EcoRV GATATC 8 cut(s) 124, 499, 556, 613, 899, 1014, 1260, 1626
EcoT14I CCWWGG 6 cut(s) 168, 282, 1146, 1383, 3153, 3201
EcoT38I GRGCYC 6 cut(s) 225, 251, 339, 365, 910, 2804
EgeI GGCGCC 1 cut(s) 2488
EheI GGCGCC 1 cut(s) 2488
ErhI CCWWGG 6 cut(s) 168, 282, 1146, 1383, 3153, 3201
Esp3I CGTCTC 1 cut(s) 2499
FaeI CATG 6 cut(s) 316, 430, 545, 860, 929, 3205
FalI AAGNNNNNCTT 2 cut(s) 2300, 2332
FaqI GGGAC 6 cut(s) 452, 779, 877, 1519, 2073, 2881
FatI CATG 6 cut(s) 312, 426, 541, 856, 925, 3201
FauI CCCGC 3 cut(s) 224, 861, 2993
FauNDI CATATG 1 cut(s) 2070
FbaI TGATCA 1 cut(s) 2821
Fnu4HI GCNGC 5 cut(s) 462, 978, 1230, 1937, 2887
FokI GGATG 5 cut(s) 2138, 2284, 2425, 2437, 3086
FriOI GRGCYC 6 cut(s) 225, 251, 339, 365, 910, 2804
Fsp4HI GCNGC 5 cut(s) 462, 978, 1230, 1937, 2887
FspBI CTAG 7 cut(s) 518, 575, 1817, 1872, 2556, 2991, 3018
GlaI GCGC 2 cut(s) 2488, 2743
GluI GCNGC 5 cut(s) 462, 978, 1230, 1937, 2887
GsaI CCCAGC 1 cut(s) 2459
GsuI CTGGAG 3 cut(s) 1023, 1506, 1635
HaeII RGCGCY 2 cut(s) 2490, 2745
HaeIII GGCC 7 cut(s) 42, 65, 583, 1232, 2404, 2560, 3152
HapII CCGG 8 cut(s) 155, 308, 606, 1300, 2132, 2745, 2805, 2865
HgaI GACGC 2 cut(s) 1796, 3173
HhaI GCGC 2 cut(s) 2489, 2744
Hin1I GRCGYC 2 cut(s) 1788, 2487
Hin1II CATG 6 cut(s) 316, 430, 545, 860, 929, 3205
Hin6I GCGC 2 cut(s) 2487, 2742
HinP1I GCGC 2 cut(s) 2487, 2742
HindIII AAGCTT 4 cut(s) 1058, 1474, 2251, 2935
HpaII CCGG 8 cut(s) 155, 308, 606, 1300, 2132, 2745, 2805, 2865
Hpy166II GTNNAC 4 cut(s) 1347, 2351, 2466, 2960
Hpy8I GTNNAC 4 cut(s) 1347, 2351, 2466, 2960
Hpy99I CGWCG 1 cut(s) 2156
HpyCH4IV ACGT 3 cut(s) 1195, 2444, 2492
HpyF10VI GCNNNNNNNGC 7 cut(s) 237, 246, 828, 977, 1207, 1819, 2456
HpySE526I ACGT 3 cut(s) 1195, 2444, 2492
Hsp92I GRCGYC 2 cut(s) 1788, 2487
Hsp92II CATG 6 cut(s) 316, 430, 545, 860, 929, 3205
HspAI GCGC 2 cut(s) 2487, 2742
KasI GGCGCC 1 cut(s) 2486
Kpn2I TCCGGA 1 cut(s) 2131
KpnI GGTACC 1 cut(s) 2283
Ksp22I TGATCA 1 cut(s) 2821
LguI GCTCTTC 1 cut(s) 2963
LmnI GCTCC 6 cut(s) 246, 360, 538, 913, 2365, 2739
Lsp1109I GCAGC 4 cut(s) 473, 964, 1923, 2898
LweI GCATC 4 cut(s) 2017, 2194, 2459, 3217
MaeI CTAG 7 cut(s) 518, 575, 1817, 1872, 2556, 2991, 3018
MaeII ACGT 3 cut(s) 1195, 2444, 2492
MaeIII GTNAC 9 cut(s) 188, 347, 1075, 1321, 1492, 1784, 2728, 2762, 2828
MflI RGATCY 7 cut(s) 147, 163, 277, 430, 640, 849, 2706
MhlI GDGCHC 7 cut(s) 225, 251, 339, 365, 756, 910, 2804
Mly113I GGCGCC 1 cut(s) 2487
MlyI GAGTC 7 cut(s) 104, 605, 1541, 1744, 1939, 2028, 2667
MroI TCCGGA 1 cut(s) 2131
MroXI GAANNNNTTC 4 cut(s) 73, 133, 1131, 2315
MseI TTAA 7 cut(s) 1235, 1308, 1674, 2055, 2622, 2789, 3089
MslI CAYNNNNRTG 4 cut(s) 546, 1683, 2835, 3054
MspA1I CMGCKG 2 cut(s) 464, 1178
MspCI CTTAAG 1 cut(s) 1234
MspI CCGG 8 cut(s) 155, 308, 606, 1300, 2132, 2745, 2805, 2865
MvaI CCWGG 4 cut(s) 44, 201, 1461, 3133
MvnI CGCG 1 cut(s) 3184
MwoI GCNNNNNNNGC 7 cut(s) 237, 246, 828, 977, 1207, 1819, 2456
NarI GGCGCC 1 cut(s) 2487
NciI CCSGG 6 cut(s) 1300, 2746, 2805, 2806, 2865, 2866
NcoI CCATGG 1 cut(s) 3201
NdeI CATATG 1 cut(s) 2070
NlaIII CATG 6 cut(s) 316, 430, 545, 860, 929, 3205
NmuCI GTSAC 7 cut(s) 347, 1075, 1321, 1784, 2728, 2762, 2828
NspV TTCGAA 1 cut(s) 2940
PceI AGGCCT 4 cut(s) 42, 2404, 2560, 3152
PciSI GCTCTTC 1 cut(s) 2963
PdmI GAANNNNTTC 4 cut(s) 73, 133, 1131, 2315
PflMI CCANNNNNTGG 2 cut(s) 818, 875
PfoI TCCNGGA 1 cut(s) 3131
PkrI GCNGC 5 cut(s) 463, 979, 1231, 1938, 2888
PleI GAGTC 7 cut(s) 103, 604, 1540, 1744, 1939, 2027, 2667
PluTI GGCGCC 1 cut(s) 2490
PpsI GAGTC 7 cut(s) 103, 604, 1540, 1744, 1939, 2027, 2667
PpuMI RGGWCCY 1 cut(s) 793
PshAI GACNNNNGTC 1 cut(s) 1276
Psp124BI GAGCTC 3 cut(s) 251, 365, 910
Psp5II RGGWCCY 1 cut(s) 793
Psp6I CCWGG 4 cut(s) 42, 199, 1459, 3131
PspFI CCCAGC 1 cut(s) 2455
PspGI CCWGG 4 cut(s) 42, 199, 1459, 3131
PspPI GGNCC 4 cut(s) 46, 415, 793, 1271
PspPPI RGGWCCY 1 cut(s) 793
PstI CTGCAG 2 cut(s) 423, 1126
PstNI CAGNNNCTG 2 cut(s) 1529, 1658
PsuI RGATCY 7 cut(s) 147, 163, 277, 430, 640, 849, 2706
PvuII CAGCTG 1 cut(s) 464
RsaI GTAC 6 cut(s) 2176, 2281, 2395, 2554, 2662, 3016
RsaNI GTAC 6 cut(s) 2175, 2280, 2394, 2553, 2661, 3015
RseI CAYNNNNRTG 4 cut(s) 546, 1683, 2835, 3054
SacI GAGCTC 3 cut(s) 251, 365, 910
SapI GCTCTTC 1 cut(s) 2963
SaqAI TTAA 7 cut(s) 1235, 1308, 1674, 2055, 2622, 2789, 3089
SatI GCNGC 5 cut(s) 462, 978, 1230, 1937, 2887
Sau96I GGNCC 4 cut(s) 46, 415, 793, 1271
SbfI CCTGCAGG 1 cut(s) 423
ScaI AGTACT 5 cut(s) 2176, 2395, 2554, 2662, 3016
SchI GAGTC 7 cut(s) 104, 605, 1541, 1744, 1939, 2028, 2667
SdaI CCTGCAGG 1 cut(s) 423
SduI GDGCHC 7 cut(s) 225, 251, 339, 365, 756, 910, 2804
SfaNI GCATC 4 cut(s) 2017, 2194, 2459, 3217
SfcI CTRYAG 3 cut(s) 419, 996, 1122
SfoI GGCGCC 1 cut(s) 2488
SfuI TTCGAA 1 cut(s) 2940
SinI GGWCC 4 cut(s) 46, 415, 793, 1271
SmaI CCCGGG 2 cut(s) 2806, 2866
SmiMI CAYNNNNRTG 4 cut(s) 546, 1683, 2835, 3054
SmlI CTYRAG 4 cut(s) 1234, 1477, 2254, 2974
SmoI CTYRAG 4 cut(s) 1234, 1477, 2254, 2974
Sse8387I CCTGCAGG 1 cut(s) 423
SseBI AGGCCT 4 cut(s) 42, 2404, 2560, 3152
SsiI CCGC 7 cut(s) 231, 854, 1176, 1229, 2389, 2448, 3000
SspDI GGCGCC 1 cut(s) 2486
SspMI CTAG 7 cut(s) 518, 575, 1817, 1872, 2556, 2991, 3018
SstI GAGCTC 3 cut(s) 251, 365, 910
StuI AGGCCT 4 cut(s) 42, 2404, 2560, 3152
StyI CCWWGG 6 cut(s) 168, 282, 1146, 1383, 3153, 3201
TaiI ACGT 3 cut(s) 1198, 2447, 2495
TaqI TCGA 8 cut(s) 77, 1712, 2154, 2287, 2398, 2563, 2940, 3102
TaqII GACCGA 1 cut(s) 1288
TatI WGTACW 5 cut(s) 2174, 2393, 2552, 2660, 3014
TauI GCSGC 1 cut(s) 1232
Tru1I TTAA 7 cut(s) 1235, 1308, 1674, 2055, 2622, 2789, 3089
Tru9I TTAA 7 cut(s) 1235, 1308, 1674, 2055, 2622, 2789, 3089
TseFI GTSAC 7 cut(s) 347, 1075, 1321, 1784, 2728, 2762, 2828
TseI GCWGC 4 cut(s) 461, 977, 1936, 2886
Tsp45I GTSAC 7 cut(s) 347, 1075, 1321, 1784, 2728, 2762, 2828
TspDTI ATGAA 9 cut(s) 707, 873, 1329, 1365, 1442, 2087, 2403, 2525, 2836
TspGWI ACGGA 6 cut(s) 158, 1024, 1340, 1793, 3038, 3236
TspMI CCCGGG 2 cut(s) 2804, 2864
Van91I CCANNNNNTGG 2 cut(s) 818, 875
Vha464I CTTAAG 1 cut(s) 1234
VpaK11BI GGWCC 4 cut(s) 46, 415, 793, 1271
XapI RAATTY 6 cut(s) 73, 1246, 1483, 2533, 2678, 2906
XcmI CCANNNNNNNNNTGG 1 cut(s) 2897
XmaI CCCGGG 2 cut(s) 2804, 2864
XmnI GAANNNNTTC 4 cut(s) 73, 133, 1131, 2315
XspI CTAG 7 cut(s) 518, 575, 1817, 1872, 2556, 2991, 3018
ZrmI AGTACT 5 cut(s) 2176, 2395, 2554, 2662, 3016
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.