Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0011050

Physical Location & Seq

Genomic Coordinates Forward (+)

34153260 .. 34154988

Gene Structure 1729 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1206700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1443 bp

ATGTCGATGGCGGAGGTCACCTTCTTCCACCTCCGCGACCCAGACGACGAGCCTCCCCACCTCACCTATCGCGCCCATCGCGACTTCGACCTCGACCTCTACTTCTCCGATCCCGATTTCCCGTCCTCCGATCGCTCCCTCCGCGCTCCCCGCATCGTCACCGTCCGCGAAGATGAATCCGACGACCCCGACGGCGACATCTTCTCCCAGTACCAATCCACCGTCCACGTCATCCGCAACGACGCCGTCTCGGGGGAGCCCAATTCCGCATCCAACCGGACCGACACATCCGGTTTGCTGAACCGCCGCGAGAACCAGGTCAATTTCGTCATGGATCTGTTCCAGCAGCGGGTCGAGCAGTCGTCTCAGGTAACCGCTCGCAGTCCCCTTTTGGTTTATGAAGATATTGACGAGGAGGATCGTAGTTTTGGGGTTATTGAGTCGAATTGCGGTGTGGGTATGGAGGGTTTGGACCTCGATTTGAGTTTAGGGTTAGGGTCGGGATTGGATTTTGGGCATTGTTTGGATGGGGACGGGATTGATGATCCCGAGGAGGAGGATTTCTTCGTGGGGAGGAGGGTTTGTAGGTCTGAATTTGGTGAGGAAACTTCCAATTTAAGTAGGGCTGATGATGATGCTTACGAGAATTGCGTGAGGCTGGTTGAGGTTGGGACCGATTCGGAGGACGATGAGAACGGGGTTATTGGGATTGATTTGAATTCCGGGGATGAGTATGGCGCGGAGGATCATGTCCATGGAGAGAATGATGTTGATACGAGCATCCCGCTGCGTTGGGATTCGCTTATTTTGGAGGACCATAGGGAAAGTAACGAAGATTTCGAGTGGGAGGAAGTTGACGGAGGGGTTGATGAGAGAGAGGTCTTCAGTATGTTTATTGCTCCCGACAATGAGGAATCAGGACCGGTGGCAGTTTCAGTTTCGGCAATGATGGCGCCGGAAGAGGAAGTGAATGTGGAGAGATATGAGCATTCGGAAAGTTTGGAATGGGAGGTTTTGTTGAATTCCAATACTTGGGACATAAATCCAGATGTTGTGCCTTATCTCGACGATGACTATATTCACACTGCAGAATATGATATGTTGTTTGGTCAATTCTCTGAGAACGAGAATGCGACAACGGGCAGGCCTCCGGCTGCCAACGCTGTGGTTGAAAGTCTCCCATCCGTAGTTTTAACTCAGGAGGATGTGGAAAAGGGTAATGCAGTCTGTGCTGTTTGCAAGGATGAGATGAAATCGGGGGAACAAGCAAAGCAGCTACCTTGCACGCATCGGTACCATGGTGACTGCATTGTGCCGTGGCTACGCATAAGGAACACATGTCCTGTTTGTCGACATGAATTGCCCTCCGATGACGCTACCTACGAGCGTAGAAGGAACCCAAGGCTTGCTCGTGGGTCGAACAATGGTCAGGCACTCTTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000902 GO:0000904 GO:0003006 GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009653 GO:0009791 GO:0009826 GO:0009888 GO:0009987 GO:0010026 GO:0010154 GO:0016043 GO:0016049 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0022412 GO:0022414 GO:0030154 GO:0032446 GO:0032501 GO:0032502 GO:0032504 GO:0032989 GO:0036211 GO:0040007 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0048316 GO:0048468 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0060560 GO:0061458 GO:0070647 GO:0071704 GO:0071840 GO:0090376 GO:0090378 GO:0090558 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

481

Amino Acids

53.99

Weight (kDa)

4.17

Isoelectric Point (pI)

44.25

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-rbx1	PF12678	408 - 451	9e-08	RING-H2 zinc finger domain
zf-RING_2	PF13639	410 - 451	7.8e-13	Ring finger domain
zf-C3HC4_2	PF13923	410 - 450	7.3e-09	Zinc finger, C3HC4 type (RING finger)
zf-RING_11	PF17123	410 - 437	8.8e-08	RING-like zinc finger
zf-C3HC4	PF00097	410 - 450	1.8e-07	Zinc finger, C3HC4 type (RING finger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011050)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G08139 AT5G08139
fragaria_vesca	FvH4_5g13270
malus_domestica	MD06G1206700.v1.1
prunus_persica	Prupe.5G211100_v2.0.a1
pyrus_communis	pycom14g18010
rosa_chinensis	RchiOBHm_Chr7g0183901
rosa_laevigata	RLG00000005013 RLG00000033786
rosa_multiflora	Rmu_sc0001926.1_g000006 Rmu_sc0004712.1_g000033 Rmu_sc0007533.1_g000005 Rmu_sc0012562.1_g000017
rosa_roxburghii	Rroxscaffold_3G00270100
rosa_rugosa	Rorug06G0461300
rosa_samantha	Rh7AG062200 Rh7BG062400 Rh7CG063800 Rh7DG062200
rosa_wichuraiana	Rw7G005140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	1293
AccB1I	GGYRCC	2 cut(s)	952, 1293
AccB7I	CCANNNNNTGG	1 cut(s)	1032
AccBSI	CCGCTC	1 cut(s)	377
AccI	GTMKAC	1 cut(s)	1351
AccII	CGCG	7 cut(s)	36, 72, 81, 144, 168, 309, 740
AclWI	GGATC	5 cut(s)	104, 342, 426, 539, 753
AcsI	RAATTY	3 cut(s)	593, 718, 1021
AcuI	CTGAAG	1 cut(s)	868
AcyI	GRCGYC	2 cut(s)	243, 953
AfaI	GTAC	2 cut(s)	212, 1295
AfiI	CCNNNNNNNGG	3 cut(s)	252, 349, 1032
AflIII	ACRYGT	1 cut(s)	1337
AgeI	ACCGGT	1 cut(s)	922
AgsI	TTSAA	3 cut(s)	718, 1021, 1172
AjiI	CACGTC	1 cut(s)	229
AjnI	CCWGG	1 cut(s)	315
AluBI	AGCT	1 cut(s)	1276
AluI	AGCT	1 cut(s)	1276
Alw26I	GTCTC	3 cut(s)	253, 369, 1181
AlwI	GGATC	5 cut(s)	104, 342, 426, 539, 753
Ama87I	CYCGRG	2 cut(s)	250, 548
AoxI	GGCC	1 cut(s)	1145
ApeKI	GCWGC	4 cut(s)	346, 787, 1154, 1273
ApoI	RAATTY	3 cut(s)	593, 718, 1021
AsiGI	ACCGGT	1 cut(s)	922
Asp718I	GGTACC	1 cut(s)	1293
AspLEI	GCGC	4 cut(s)	74, 146, 740, 955
AspS9I	GGNCC	5 cut(s)	279, 472, 672, 814, 920
AsuC2I	CCSGG	1 cut(s)	724
AsuHPI	GGTGA	5 cut(s)	10, 55, 151, 611, 1313
AvaI	CYCGRG	2 cut(s)	250, 548
AvaII	GGWCC	5 cut(s)	279, 472, 672, 814, 920
BaeI	ACNNNNGTAYC	2 cut(s)	1285, 1318
BanI	GGYRCC	2 cut(s)	952, 1293
BanII	GRGCYC	1 cut(s)	261
BauI	CACGAG	1 cut(s)	1410
BbsI	GAAGAC	1 cut(s)	874
BbvI	GCAGC	4 cut(s)	358, 774, 1141, 1285
BccI	CCATC	4 cut(s)	84, 521, 943, 1189
BceAI	ACGGC	3 cut(s)	208, 230, 1300
BciT130I	CCWGG	1 cut(s)	317
BcnI	CCSGG	1 cut(s)	724
BcoDI	GTCTC	3 cut(s)	253, 369, 1181
BfmI	CTRYAG	1 cut(s)	1086
BfoI	RGCGCY	1 cut(s)	956
BisI	GCNGC	5 cut(s)	307, 347, 788, 1155, 1274
BlsI	GCNGC	5 cut(s)	308, 348, 789, 1156, 1275
Bme1390I	CCNGG	2 cut(s)	317, 724
Bme18I	GGWCC	5 cut(s)	279, 472, 672, 814, 920
BmeT110I	CYCGRG	2 cut(s)	250, 548
BmgBI	CACGTC	1 cut(s)	229
BmgT120I	GGNCC	5 cut(s)	279, 472, 672, 814, 920
BmiI	GGNNCC	5 cut(s)	258, 673, 954, 1295, 1397
BmrFI	CCNGG	2 cut(s)	317, 724
BmrI	ACTGGG	1 cut(s)	202
BmsI	GCATC	5 cut(s)	162, 278, 625, 789, 1297
BmuI	ACTGGG	1 cut(s)	202
BpiI	GAAGAC	1 cut(s)	874
BpuMI	CCSGG	1 cut(s)	724
BsaHI	GRCGYC	2 cut(s)	243, 953
BsaJI	CCNNGG	6 cut(s)	549, 723, 754, 1297, 1316, 1400
BsaWI	WCCGGW	3 cut(s)	276, 290, 922
BsaXI	ACNNNNNCTCC	6 cut(s)	188, 218, 568, 598, 1001, 1031
Bsc4I	CCNNNNNNNGG	3 cut(s)	252, 349, 1032
Bse118I	RCCGGY	1 cut(s)	922
Bse1I	ACTGG	1 cut(s)	208
Bse3DI	GCAATG	1 cut(s)	951
BseBI	CCWGG	1 cut(s)	317
BseDI	CCNNGG	6 cut(s)	549, 723, 754, 1297, 1316, 1400
BseGI	GGATG	9 cut(s)	231, 269, 287, 532, 733, 780, 1181, 1210, 1249
BseLI	CCNNNNNNNGG	3 cut(s)	252, 349, 1032
BseMI	GCAATG	1 cut(s)	951
BseMII	CTCAG	3 cut(s)	380, 1110, 1211
BseNI	ACTGG	1 cut(s)	208
BseRI	GAGGAG	4 cut(s)	428, 566, 569, 589
BseXI	GCAGC	4 cut(s)	358, 774, 1141, 1285
Bsh1236I	CGCG	7 cut(s)	36, 72, 81, 144, 168, 309, 740
Bsh1285I	CGRYCG	1 cut(s)	133
BshFI	GGCC	1 cut(s)	1147
BshNI	GGYRCC	2 cut(s)	952, 1293
BshTI	ACCGGT	1 cut(s)	922
BsiEI	CGRYCG	1 cut(s)	133
BsiHKCI	CYCGRG	2 cut(s)	250, 548
BsiSI	CCGG	6 cut(s)	277, 291, 723, 923, 956, 1151
BslFI	GGGAC	4 cut(s)	369, 545, 685, 1049
BslI	CCNNNNNNNGG	3 cut(s)	252, 349, 1032
BsmAI	GTCTC	3 cut(s)	253, 369, 1181
BsmBI	CGTCTC	2 cut(s)	253, 369
BsmFI	GGGAC	4 cut(s)	369, 545, 685, 1049
BsmI	GAATGC	2 cut(s)	988, 1135
BsnI	GGCC	1 cut(s)	1147
BsoBI	CYCGRG	2 cut(s)	250, 548
Bsp1286I	GDGCHC	1 cut(s)	261
Bsp143I	GATC	6 cut(s)	109, 130, 334, 418, 544, 745
Bsp19I	CCATGG	2 cut(s)	754, 1297
Bsp68I	TCGCGA	1 cut(s)	81
BspANI	GGCC	1 cut(s)	1147
BspCNI	CTCAG	3 cut(s)	379, 1111, 1210
BspFNI	CGCG	7 cut(s)	36, 72, 81, 144, 168, 309, 740
BspLI	GGNNCC	5 cut(s)	258, 673, 954, 1295, 1397
BspMAI	CTGCAG	1 cut(s)	1090
BspPI	GGATC	5 cut(s)	104, 342, 426, 539, 753
BspT107I	GGYRCC	2 cut(s)	952, 1293
BsrBI	CCGCTC	1 cut(s)	377
BsrDI	GCAATG	1 cut(s)	951
BsrFI	RCCGGY	1 cut(s)	922
BsrI	ACTGG	1 cut(s)	208
BssAI	RCCGGY	1 cut(s)	922
BssECI	CCNNGG	6 cut(s)	549, 723, 754, 1297, 1316, 1400
BssMI	GATC	6 cut(s)	109, 130, 334, 418, 544, 745
BssNI	GRCGYC	2 cut(s)	243, 953
BssSI	CACGAG	1 cut(s)	1410
BssT1I	CCWWGG	3 cut(s)	754, 1297, 1400
Bst2BI	CACGAG	1 cut(s)	1410
Bst2UI	CCWGG	1 cut(s)	317
Bst4CI	ACNGT	2 cut(s)	163, 223
Bst6I	CTCTTC	1 cut(s)	954
BstACI	GRCGYC	2 cut(s)	243, 953
BstAPI	GCANNNNNTGC	1 cut(s)	1229
BstC8I	GCNNGC	4 cut(s)	379, 1145, 1286, 1407
BstDEI	CTNAG	3 cut(s)	366, 1119, 1197
BstDSI	CCRYGG	3 cut(s)	754, 1297, 1316
BstEII	GGTNACC	2 cut(s)	16, 370
BstF5I	GGATG	9 cut(s)	231, 269, 287, 532, 733, 780, 1181, 1210, 1249
BstFNI	CGCG	7 cut(s)	36, 72, 81, 144, 168, 309, 740
BstH2I	RGCGCY	1 cut(s)	956
BstHHI	GCGC	4 cut(s)	74, 146, 740, 955
BstKTI	GATC	6 cut(s)	112, 133, 337, 421, 547, 748
BstMAI	GTCTC	3 cut(s)	253, 369, 1181
BstMBI	GATC	6 cut(s)	109, 130, 334, 418, 544, 745
BstMCI	CGRYCG	1 cut(s)	133
BstMWI	GCNNNNNNNGC	7 cut(s)	78, 141, 150, 355, 950, 1160, 1229
BstNI	CCWGG	1 cut(s)	317
BstNSI	RCATGY	1 cut(s)	1341
BstPI	GGTNACC	2 cut(s)	16, 370
BstSCI	CCNGG	2 cut(s)	315, 722
BstSFI	CTRYAG	1 cut(s)	1086
BstUI	CGCG	7 cut(s)	36, 72, 81, 144, 168, 309, 740
BstV1I	GCAGC	4 cut(s)	358, 774, 1141, 1285
BstV2I	GAAGAC	1 cut(s)	874
BstX2I	RGATCY	1 cut(s)	334
BstXI	CCANNNNNNTGG	1 cut(s)	1165
BstYI	RGATCY	1 cut(s)	334
BsuRI	GGCC	1 cut(s)	1147
BtgI	CCRYGG	3 cut(s)	754, 1297, 1316
BtgZI	GCGATG	1 cut(s)	62
BtrI	CACGTC	1 cut(s)	229
BtsCI	GGATG	9 cut(s)	231, 269, 287, 532, 733, 780, 1181, 1210, 1249
BtsI	GCAGTG	1 cut(s)	1083
BtsIMutI	CAGTG	1 cut(s)	1083
BtuMI	TCGCGA	1 cut(s)	81
Cac8I	GCNNGC	4 cut(s)	379, 1145, 1286, 1407
CfoI	GCGC	4 cut(s)	74, 146, 740, 955
Cfr10I	RCCGGY	1 cut(s)	922
Cfr13I	GGNCC	5 cut(s)	279, 472, 672, 814, 920
CpoI	CGGWCCG	1 cut(s)	279
CseI	GACGC	2 cut(s)	251, 1382
CsiI	ACCWGGT	1 cut(s)	315
Csp6I	GTAC	2 cut(s)	211, 1294
CspAI	ACCGGT	1 cut(s)	922
CspI	CGGWCCG	1 cut(s)	279
CviAII	CATG	6 cut(s)	331, 749, 755, 1298, 1338, 1355
CviJI	RGCY	9 cut(s)	52, 259, 626, 658, 1147, 1154, 1276, 1321, 1405
CviKI_1	RGCY	9 cut(s)	52, 259, 626, 658, 1147, 1154, 1276, 1321, 1405
CviQI	GTAC	2 cut(s)	211, 1294
DdeI	CTNAG	3 cut(s)	366, 1119, 1197
DinI	GGCGCC	1 cut(s)	954
DpnI	GATC	6 cut(s)	111, 132, 336, 420, 546, 747
DpnII	GATC	6 cut(s)	109, 130, 334, 418, 544, 745
Eam1104I	CTCTTC	1 cut(s)	954
EarI	CTCTTC	1 cut(s)	954
EciI	GGCGGA	1 cut(s)	26
Eco130I	CCWWGG	3 cut(s)	754, 1297, 1400
Eco147I	AGGCCT	1 cut(s)	1147
Eco24I	GRGCYC	1 cut(s)	261
Eco47I	GGWCC	5 cut(s)	279, 472, 672, 814, 920
Eco57I	CTGAAG	1 cut(s)	868
Eco88I	CYCGRG	2 cut(s)	250, 548
Eco91I	GGTNACC	2 cut(s)	16, 370
EcoO65I	GGTNACC	2 cut(s)	16, 370
EcoRI	GAATTC	2 cut(s)	718, 1021
EcoRII	CCWGG	1 cut(s)	315
EcoT14I	CCWWGG	3 cut(s)	754, 1297, 1400
EcoT38I	GRGCYC	1 cut(s)	261
EgeI	GGCGCC	1 cut(s)	954
EheI	GGCGCC	1 cut(s)	954
ErhI	CCWWGG	3 cut(s)	754, 1297, 1400
Esp3I	CGTCTC	2 cut(s)	253, 369
FaeI	CATG	6 cut(s)	334, 752, 758, 1301, 1341, 1358
FaqI	GGGAC	4 cut(s)	369, 545, 685, 1049
FatI	CATG	6 cut(s)	330, 748, 754, 1297, 1337, 1354
FauI	CCCGC	3 cut(s)	158, 342, 792
FblI	GTMKAC	1 cut(s)	1351
Fnu4HI	GCNGC	5 cut(s)	307, 347, 788, 1155, 1274
FokI	GGATG	9 cut(s)	218, 256, 274, 539, 740, 767, 1168, 1217, 1256
FriOI	GRGCYC	1 cut(s)	261
Fsp4HI	GCNGC	5 cut(s)	307, 347, 788, 1155, 1274
GlaI	GCGC	4 cut(s)	73, 145, 739, 954
GluI	GCNGC	5 cut(s)	307, 347, 788, 1155, 1274
HaeII	RGCGCY	1 cut(s)	956
HaeIII	GGCC	1 cut(s)	1147
HapII	CCGG	6 cut(s)	277, 291, 723, 923, 956, 1151
HgaI	GACGC	2 cut(s)	251, 1382
HhaI	GCGC	4 cut(s)	74, 146, 740, 955
Hin1I	GRCGYC	2 cut(s)	243, 953
Hin1II	CATG	6 cut(s)	334, 752, 758, 1301, 1341, 1358
Hin6I	GCGC	4 cut(s)	72, 144, 738, 953
HinP1I	GCGC	4 cut(s)	72, 144, 738, 953
HincII	GTYRAC	2 cut(s)	856, 1352
HindII	GTYRAC	2 cut(s)	856, 1352
HinfI	GANTC	5 cut(s)	176, 440, 677, 797, 914
HpaII	CCGG	6 cut(s)	277, 291, 723, 923, 956, 1151
HphI	GGTGA	5 cut(s)	10, 55, 151, 611, 1313
Hpy166II	GTNNAC	3 cut(s)	226, 856, 1352
Hpy188I	TCNGA	8 cut(s)	109, 130, 181, 592, 682, 994, 1120, 1369
Hpy188III	TCNNGA	9 cut(s)	80, 113, 501, 548, 902, 918, 1046, 1064, 1199
Hpy8I	GTNNAC	3 cut(s)	226, 856, 1352
Hpy99I	CGWCG	5 cut(s)	50, 185, 194, 245, 1070
HpyAV	CCTTC	2 cut(s)	31, 1386
HpyCH4III	ACNGT	2 cut(s)	163, 223
HpyCH4IV	ACGT	1 cut(s)	228
HpyCH4V	TGCA	5 cut(s)	1088, 1223, 1239, 1284, 1308
HpyF10VI	GCNNNNNNNGC	7 cut(s)	78, 141, 150, 355, 950, 1160, 1229
HpyF3I	CTNAG	3 cut(s)	366, 1119, 1197
HpySE526I	ACGT	1 cut(s)	228
Hsp92I	GRCGYC	2 cut(s)	243, 953
Hsp92II	CATG	6 cut(s)	334, 752, 758, 1301, 1341, 1358
HspAI	GCGC	4 cut(s)	72, 144, 738, 953
KasI	GGCGCC	1 cut(s)	952
KpnI	GGTACC	1 cut(s)	1297
Kzo9I	GATC	6 cut(s)	109, 130, 334, 418, 544, 745
LmnI	GCTCC	4 cut(s)	140, 151, 256, 904
Lsp1109I	GCAGC	4 cut(s)	358, 774, 1141, 1285
LweI	GCATC	5 cut(s)	162, 278, 625, 789, 1297
MabI	ACCWGGT	1 cut(s)	315
MaeII	ACGT	1 cut(s)	228
MaeIII	GTNAC	5 cut(s)	16, 157, 370, 827, 1301
MalI	GATC	6 cut(s)	111, 132, 336, 420, 546, 747
MbiI	CCGCTC	1 cut(s)	377
MboI	GATC	6 cut(s)	109, 130, 334, 418, 544, 745
MboII	GAAGA	8 cut(s)	16, 182, 193, 413, 556, 845, 874, 971
MflI	RGATCY	1 cut(s)	334
MhlI	GDGCHC	1 cut(s)	261
Mly113I	GGCGCC	1 cut(s)	953
MlyI	GAGTC	1 cut(s)	449
MmeI	TCCRAC	2 cut(s)	204, 297
MseI	TTAA	2 cut(s)	617, 1193
MslI	CAYNNNNRTG	1 cut(s)	753
MspA1I	CMGCKG	2 cut(s)	349, 787
MspI	CCGG	6 cut(s)	277, 291, 723, 923, 956, 1151
MspR9I	CCNGG	2 cut(s)	317, 724
Mva1269I	GAATGC	2 cut(s)	988, 1135
MvaI	CCWGG	1 cut(s)	317
MvnI	CGCG	7 cut(s)	36, 72, 81, 144, 168, 309, 740
MwoI	GCNNNNNNNGC	7 cut(s)	78, 141, 150, 355, 950, 1160, 1229
NarI	GGCGCC	1 cut(s)	953
NciI	CCSGG	1 cut(s)	724
NcoI	CCATGG	2 cut(s)	754, 1297
NdeII	GATC	6 cut(s)	109, 130, 334, 418, 544, 745
NlaIII	CATG	6 cut(s)	334, 752, 758, 1301, 1341, 1358
NlaIV	GGNNCC	5 cut(s)	258, 673, 954, 1295, 1397
NmuCI	GTSAC	3 cut(s)	16, 157, 1301
NruI	TCGCGA	1 cut(s)	81
NspI	RCATGY	1 cut(s)	1341
PceI	AGGCCT	1 cut(s)	1147
PciI	ACATGT	1 cut(s)	1337
PcsI	WCGNNNNNNNCGW	3 cut(s)	648, 837, 1380
PctI	GAATGC	2 cut(s)	988, 1135
PfeI	GAWTC	4 cut(s)	176, 677, 797, 914
PflFI	GACNNNGTC	1 cut(s)	245
PflMI	CCANNNNNTGG	1 cut(s)	1032
PinAI	ACCGGT	1 cut(s)	922
PkrI	GCNGC	5 cut(s)	308, 348, 789, 1156, 1275
Ple19I	CGATCG	1 cut(s)	133
PleI	GAGTC	1 cut(s)	448
PluTI	GGCGCC	1 cut(s)	956
PpsI	GAGTC	1 cut(s)	448
PscI	ACATGT	1 cut(s)	1337
Psp6I	CCWGG	1 cut(s)	315
PspEI	GGTNACC	2 cut(s)	16, 370
PspGI	CCWGG	1 cut(s)	315
PspN4I	GGNNCC	5 cut(s)	258, 673, 954, 1295, 1397
PspPI	GGNCC	5 cut(s)	279, 472, 672, 814, 920
PstI	CTGCAG	1 cut(s)	1090
PsuI	RGATCY	1 cut(s)	334
PsyI	GACNNNGTC	1 cut(s)	245
PvuI	CGATCG	1 cut(s)	133
RruI	TCGCGA	1 cut(s)	81
RsaI	GTAC	2 cut(s)	212, 1295
RsaNI	GTAC	2 cut(s)	211, 1294
RseI	CAYNNNNRTG	1 cut(s)	753
Rsr2I	CGGWCCG	1 cut(s)	279
RsrII	CGGWCCG	1 cut(s)	279
SalI	GTCGAC	1 cut(s)	1350
SaqAI	TTAA	2 cut(s)	617, 1193
SatI	GCNGC	5 cut(s)	307, 347, 788, 1155, 1274
Sau3AI	GATC	6 cut(s)	109, 130, 334, 418, 544, 745
Sau96I	GGNCC	5 cut(s)	279, 472, 672, 814, 920
SchI	GAGTC	1 cut(s)	449
ScrFI	CCNGG	2 cut(s)	317, 724
SduI	GDGCHC	1 cut(s)	261
SexAI	ACCWGGT	1 cut(s)	315
SfaNI	GCATC	5 cut(s)	162, 278, 625, 789, 1297
SfcI	CTRYAG	1 cut(s)	1086
SfoI	GGCGCC	1 cut(s)	954
SinI	GGWCC	5 cut(s)	279, 472, 672, 814, 920
SmiMI	CAYNNNNRTG	1 cut(s)	753
SseBI	AGGCCT	1 cut(s)	1147
SspDI	GGCGCC	1 cut(s)	952
StuI	AGGCCT	1 cut(s)	1147
StyD4I	CCNGG	2 cut(s)	315, 722
StyI	CCWWGG	3 cut(s)	754, 1297, 1400
TaaI	ACNGT	2 cut(s)	163, 223
TaiI	ACGT	1 cut(s)	231
TaqII	GACCGA	2 cut(s)	296, 689
TauI	GCSGC	1 cut(s)	309
TfiI	GAWTC	4 cut(s)	176, 677, 797, 914
Tru1I	TTAA	2 cut(s)	617, 1193
Tru9I	TTAA	2 cut(s)	617, 1193
TscAI	CASTG	1 cut(s)	1090
TseFI	GTSAC	3 cut(s)	16, 157, 1301
TseI	GCWGC	4 cut(s)	346, 787, 1154, 1273
Tsp45I	GTSAC	3 cut(s)	16, 157, 1301
TspDTI	ATGAA	4 cut(s)	189, 414, 1265, 1371
TspGWI	ACGGA	2 cut(s)	873, 1174
TspRI	CASTG	1 cut(s)	1090
Tth111I	GACNNNGTC	1 cut(s)	245
Van91I	CCANNNNNTGG	1 cut(s)	1032
VpaK11BI	GGWCC	5 cut(s)	279, 472, 672, 814, 920
XapI	RAATTY	3 cut(s)	593, 718, 1021
XceI	RCATGY	1 cut(s)	1341
XmiI	GTMKAC	1 cut(s)	1351

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.