Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr14

Orthogroup ID

OG0011050

Physical Location & Seq

Genomic Coordinates Forward (+)

19813538 .. 19814956

Gene Structure 1419 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom14g18010.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1419 bp

ATGTCGATGGCGGAGGTCACCTTCTTCCAACTCCACGACGTCGACGACGACCCTCCCCACCTCCCCTACTGGGCACACCAGGACCTTGACCTCTACTCCTCCGATCCCGATTTCCCTCAATCCGATCGCGCCCTCCGCCCCTCCCGCATCGTCACCGTCGACGACGACGGCGACCTCTTCTCCCAGTACCAGTCCCCCGTCCACGTCATCCGCAACGACGCCGTTTTGGGGGAGCCCGATTCCGGATCCAACCTGACCAACCGATCGGATATGCTGGACCGCCGCGAGGACCAGGTGAATTTCGTGATGGATCTGTTCCAGCAGCGGGTCGAGCAGTCGTCTCAGGTAAACGCTCGCAGTCCCCTTTTGGTTTCTGAAGATATTGACGACGAGGATCGTAGTTTTGGGGTTATTGAGTCGAATTGTGATGTGGGTATGGAGGGTTTGGACCTCGATTTGAGTTTAGGGTTAGGGTCGGGATTGGATTTTGGGAATTGTTTGGATGGGGACGGGATTGATGATCCCGAGGAGGATGATTTCTTTGTGGGGAGGAGGGTGTCTGGGTCTGAATTTGGTGAGGAATCTTCCAATTTAAGTAGGGCAGATGATGATGCTTACGAGAATTGCGTGAGGCTGGTTGGGTTCGGGACCGATTCGGAGGATGATGAGAACGGGGTTATCGGGATCGATTTGAATTCCGGGGATGAGTATGGCGTGCAGGGTCGTGTCCATGGTGAGAATGATGATGATACGAGCATCCCGCTGCGTTGGGATTCGTTTATTTTGGAGGACCATAGGGAAACTAACGAAGATTTCGAGTGGGAGGAAGTTGAAGACGGGGTTGATGAGAGAGAGGTCTTCAGTACGTTTATTGATCATGACATCGAGGAATCAGGACCGGTGGCAGTTTCAGTTTTGGCAATGGTGGCACCGGAAGAGGAAATGACTGTGGAGAGATCCGAGCATGTGGATAGTTTGGAATGGGAAATTTTGTTGAATTCCAATACTTGGGAGATAAATCCAGATGTGGCGCCTTATCCGGACGATGATTATATTCACACTGCAGAATATGATTTGCTGTTTGGTCAATTCTCTGAGAATGAGAATGCAACAGCGGGCAGGCCTCCGGCTGCCAACGCTGTGGTTGAAGGTCTCCCATCCGTAGTTTTAACTCAGGAGGATGTGGAAAAGGGCAATGCACTCTGTGCCGTTTGCAAGGATGAGATGAAATCGGGGGAACAAGCAAAGCAGCTACCTTGCACACATCGGTACCATGGTGATTGCATTGTGCCGTGGCTGCGCATAAGGAACACATGTCCTGTTTGTCGACATGAATTGCCCACCGCTGACGCTGCTTACGAGCGTAGAAGGAACCAAAGGCAAGCTCGTGGGTCGAACGATGGGCGGGCAATGTTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000902 GO:0000904 GO:0003006 GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009653 GO:0009791 GO:0009826 GO:0009888 GO:0009987 GO:0010026 GO:0010154 GO:0016043 GO:0016049 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0022412 GO:0022414 GO:0030154 GO:0032446 GO:0032501 GO:0032502 GO:0032504 GO:0032989 GO:0036211 GO:0040007 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0048316 GO:0048468 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0060560 GO:0061458 GO:0070647 GO:0071704 GO:0071840 GO:0090376 GO:0090378 GO:0090558 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

473

Amino Acids

52.9

Weight (kDa)

4.08

Isoelectric Point (pI)

49.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-rbx1	PF12678	400 - 443	8.6e-08	RING-H2 zinc finger domain
zf-RING_2	PF13639	401 - 443	6.8e-13	Ring finger domain
zf-C3HC4_2	PF13923	402 - 442	1.1e-08	Zinc finger, C3HC4 type (RING finger)
zf-RING_11	PF17123	402 - 429	8.7e-08	RING-like zinc finger
zf-C3HC4	PF00097	402 - 442	1.8e-07	Zinc finger, C3HC4 type (RING finger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011050)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G08139 AT5G08139
fragaria_vesca	FvH4_5g13270
malus_domestica	MD06G1206700.v1.1
prunus_persica	Prupe.5G211100_v2.0.a1
pyrus_communis	pycom14g18010
rosa_chinensis	RchiOBHm_Chr7g0183901
rosa_laevigata	RLG00000005013 RLG00000033786
rosa_multiflora	Rmu_sc0001926.1_g000006 Rmu_sc0004712.1_g000033 Rmu_sc0007533.1_g000005 Rmu_sc0012562.1_g000017
rosa_roxburghii	Rroxscaffold_3G00270100
rosa_rugosa	Rorug06G0461300
rosa_samantha	Rh7AG062200 Rh7BG062400 Rh7CG063800 Rh7DG062200
rosa_wichuraiana	Rw7G005140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	42
Acc16I	TGCGCA	1 cut(s)	1301
Acc65I	GGTACC	1 cut(s)	1269
AccB1I	GGYRCC	3 cut(s)	928, 1030, 1269
AccB7I	CCANNNNNTGG	1 cut(s)	1008
AccI	GTMKAC	3 cut(s)	42, 159, 1327
AccII	CGCG	2 cut(s)	129, 285
AccIII	TCCGGA	2 cut(s)	242, 1039
AclWI	GGATC	8 cut(s)	98, 240, 253, 318, 402, 515, 692, 951
AcsI	RAATTY	5 cut(s)	298, 569, 694, 987, 997
AcuI	CTGAAG	2 cut(s)	396, 844
AcyI	GRCGYC	3 cut(s)	39, 219, 1031
AdeI	CACNNNGTG	1 cut(s)	1205
AfaI	GTAC	3 cut(s)	188, 865, 1271
AfiI	CCNNNNNNNGG	6 cut(s)	70, 228, 242, 286, 325, 1008
AflIII	ACRYGT	1 cut(s)	1313
AgeI	ACCGGT	1 cut(s)	898
AgsI	TTSAA	4 cut(s)	694, 833, 997, 1148
AjiI	CACGTC	1 cut(s)	205
AjnI	CCWGG	2 cut(s)	78, 291
AluBI	AGCT	2 cut(s)	1252, 1385
AluI	AGCT	2 cut(s)	1252, 1385
Alw26I	GTCTC	2 cut(s)	345, 1157
AlwI	GGATC	8 cut(s)	98, 240, 253, 318, 402, 515, 692, 951
Ama87I	CYCGRG	1 cut(s)	524
Aor13HI	TCCGGA	2 cut(s)	242, 1039
AoxI	GGCC	1 cut(s)	1121
ApeKI	GCWGC	6 cut(s)	322, 763, 1130, 1249, 1297, 1352
ApoI	RAATTY	5 cut(s)	298, 569, 694, 987, 997
AsiGI	ACCGGT	1 cut(s)	898
Asp718I	GGTACC	1 cut(s)	1269
AspLEI	GCGC	3 cut(s)	131, 1033, 1302
AspS9I	GGNCC	7 cut(s)	82, 277, 289, 448, 648, 790, 896
AsuC2I	CCSGG	1 cut(s)	700
AsuHPI	GGTGA	6 cut(s)	10, 145, 307, 587, 746, 1289
AvaI	CYCGRG	1 cut(s)	524
AvaII	GGWCC	7 cut(s)	82, 277, 289, 448, 648, 790, 896
BaeGI	GKGCMC	1 cut(s)	76
BaeI	ACNNNNGTAYC	4 cut(s)	1253, 1261, 1286, 1294
BamHI	GGATCC	1 cut(s)	245
BanI	GGYRCC	3 cut(s)	928, 1030, 1269
BanII	GRGCYC	1 cut(s)	237
BauI	CACGAG	1 cut(s)	1386
BbsI	GAAGAC	2 cut(s)	840, 850
BbvI	GCAGC	6 cut(s)	334, 750, 1117, 1261, 1284, 1339
BccI	CCATC	4 cut(s)	301, 497, 1165, 1394
BceAI	ACGGC	4 cut(s)	184, 206, 1193, 1276
BciT130I	CCWGG	2 cut(s)	80, 293
BclI	TGATCA	1 cut(s)	874
BcnI	CCSGG	1 cut(s)	700
BcoDI	GTCTC	2 cut(s)	345, 1157
BfmI	CTRYAG	1 cut(s)	1062
BfoI	RGCGCY	1 cut(s)	1034
BisI	GCNGC	7 cut(s)	283, 323, 764, 1131, 1250, 1298, 1353
BlsI	GCNGC	7 cut(s)	284, 324, 765, 1132, 1251, 1299, 1354
Bme1390I	CCNGG	3 cut(s)	80, 293, 700
Bme18I	GGWCC	7 cut(s)	82, 277, 289, 448, 648, 790, 896
BmeT110I	CYCGRG	1 cut(s)	524
BmgBI	CACGTC	1 cut(s)	205
BmgT120I	GGNCC	7 cut(s)	82, 277, 289, 448, 648, 790, 896
BmiI	GGNNCC	7 cut(s)	234, 247, 649, 930, 1032, 1271, 1373
BmrFI	CCNGG	3 cut(s)	80, 293, 700
BmrI	ACTGGG	2 cut(s)	79, 178
BmsI	GCATC	3 cut(s)	156, 601, 765
BmuI	ACTGGG	2 cut(s)	79, 178
BpiI	GAAGAC	2 cut(s)	840, 850
BpuMI	CCSGG	1 cut(s)	700
Bsa29I	ATCGAT	1 cut(s)	687
BsaHI	GRCGYC	3 cut(s)	39, 219, 1031
BsaI	GGTCTC	1 cut(s)	1157
BsaJI	CCNNGG	5 cut(s)	525, 699, 730, 1273, 1292
BsaWI	WCCGGW	4 cut(s)	242, 898, 931, 1039
BsaXI	ACNNNNNCTCC	4 cut(s)	80, 110, 164, 194
Bsc4I	CCNNNNNNNGG	6 cut(s)	70, 228, 242, 286, 325, 1008
Bse118I	RCCGGY	1 cut(s)	898
Bse1I	ACTGG	3 cut(s)	74, 184, 190
Bse3DI	GCAATG	3 cut(s)	927, 1201, 1416
BseAI	TCCGGA	2 cut(s)	242, 1039
BseBI	CCWGG	2 cut(s)	80, 293
BseCI	ATCGAT	1 cut(s)	687
BseDI	CCNNGG	5 cut(s)	525, 699, 730, 1273, 1292
BseGI	GGATG	9 cut(s)	207, 508, 538, 667, 709, 756, 1157, 1186, 1225
BseLI	CCNNNNNNNGG	6 cut(s)	70, 228, 242, 286, 325, 1008
BseMI	GCAATG	3 cut(s)	927, 1201, 1416
BseMII	CTCAG	3 cut(s)	356, 1086, 1187
BseNI	ACTGG	3 cut(s)	74, 184, 190
BseRI	GAGGAG	3 cut(s)	88, 542, 565
BseSI	GKGCMC	1 cut(s)	76
BseXI	GCAGC	6 cut(s)	334, 750, 1117, 1261, 1284, 1339
BsgI	GTGCAG	1 cut(s)	737
Bsh1236I	CGCG	2 cut(s)	129, 285
Bsh1285I	CGRYCG	2 cut(s)	127, 266
BshFI	GGCC	1 cut(s)	1123
BshNI	GGYRCC	3 cut(s)	928, 1030, 1269
BshTI	ACCGGT	1 cut(s)	898
BshVI	ATCGAT	1 cut(s)	687
BsiEI	CGRYCG	2 cut(s)	127, 266
BsiHKCI	CYCGRG	1 cut(s)	524
BsiSI	CCGG	6 cut(s)	243, 699, 899, 932, 1040, 1127
BslFI	GGGAC	4 cut(s)	178, 345, 521, 661
BslI	CCNNNNNNNGG	6 cut(s)	70, 228, 242, 286, 325, 1008
BsmAI	GTCTC	2 cut(s)	345, 1157
BsmBI	CGTCTC	1 cut(s)	345
BsmFI	GGGAC	4 cut(s)	178, 345, 521, 661
BsmI	GAATGC	1 cut(s)	1111
BsnI	GGCC	1 cut(s)	1123
Bso31I	GGTCTC	1 cut(s)	1157
BsoBI	CYCGRG	1 cut(s)	524
Bsp1286I	GDGCHC	2 cut(s)	76, 237
Bsp13I	TCCGGA	2 cut(s)	242, 1039
Bsp19I	CCATGG	2 cut(s)	730, 1273
BspANI	GGCC	1 cut(s)	1123
BspCNI	CTCAG	3 cut(s)	355, 1087, 1186
BspDI	ATCGAT	1 cut(s)	687
BspEI	TCCGGA	2 cut(s)	242, 1039
BspFNI	CGCG	2 cut(s)	129, 285
BspHI	TCATGA	1 cut(s)	877
BspLI	GGNNCC	7 cut(s)	234, 247, 649, 930, 1032, 1271, 1373
BspMAI	CTGCAG	1 cut(s)	1066
BspPI	GGATC	8 cut(s)	98, 240, 253, 318, 402, 515, 692, 951
BspT107I	GGYRCC	3 cut(s)	928, 1030, 1269
BspTNI	GGTCTC	1 cut(s)	1157
BsrDI	GCAATG	3 cut(s)	927, 1201, 1416
BsrFI	RCCGGY	1 cut(s)	898
BsrI	ACTGG	3 cut(s)	74, 184, 190
BssAI	RCCGGY	1 cut(s)	898
BssECI	CCNNGG	5 cut(s)	525, 699, 730, 1273, 1292
BssNI	GRCGYC	3 cut(s)	39, 219, 1031
BssSI	CACGAG	1 cut(s)	1386
BssT1I	CCWWGG	2 cut(s)	730, 1273
Bst2BI	CACGAG	1 cut(s)	1386
Bst2UI	CCWGG	2 cut(s)	80, 293
Bst4CI	ACNGT	2 cut(s)	157, 949
Bst6I	CTCTTC	2 cut(s)	182, 930
BstACI	GRCGYC	3 cut(s)	39, 219, 1031
BstAPI	GCANNNNNTGC	1 cut(s)	1205
BstC8I	GCNNGC	6 cut(s)	355, 716, 1117, 1121, 1383, 1407
BstDEI	CTNAG	3 cut(s)	342, 1095, 1173
BstDSI	CCRYGG	3 cut(s)	730, 1273, 1292
BstEII	GGTNACC	1 cut(s)	16
BstF5I	GGATG	9 cut(s)	207, 508, 538, 667, 709, 756, 1157, 1186, 1225
BstFNI	CGCG	2 cut(s)	129, 285
BstH2I	RGCGCY	1 cut(s)	1034
BstHHI	GCGC	3 cut(s)	131, 1033, 1302
BstMAI	GTCTC	2 cut(s)	345, 1157
BstMCI	CGRYCG	2 cut(s)	127, 266
BstMWI	GCNNNNNNNGC	8 cut(s)	135, 144, 331, 926, 1136, 1205, 1297, 1352
BstNI	CCWGG	2 cut(s)	80, 293
BstNSI	RCATGY	2 cut(s)	968, 1317
BstPI	GGTNACC	1 cut(s)	16
BstSCI	CCNGG	3 cut(s)	78, 291, 698
BstSFI	CTRYAG	1 cut(s)	1062
BstSLI	GKGCMC	1 cut(s)	76
BstUI	CGCG	2 cut(s)	129, 285
BstV1I	GCAGC	6 cut(s)	334, 750, 1117, 1261, 1284, 1339
BstV2I	GAAGAC	2 cut(s)	840, 850
BstX2I	RGATCY	3 cut(s)	245, 310, 956
BstXI	CCANNNNNNTGG	1 cut(s)	1141
BstYI	RGATCY	3 cut(s)	245, 310, 956
Bsu15I	ATCGAT	1 cut(s)	687
BsuRI	GGCC	1 cut(s)	1123
BsuTUI	ATCGAT	1 cut(s)	687
BtgI	CCRYGG	3 cut(s)	730, 1273, 1292
BtrI	CACGTC	1 cut(s)	205
BtsCI	GGATG	9 cut(s)	207, 508, 538, 667, 709, 756, 1157, 1186, 1225
BtsI	GCAGTG	1 cut(s)	1059
BtsIMutI	CAGTG	1 cut(s)	1059
Cac8I	GCNNGC	6 cut(s)	355, 716, 1117, 1121, 1383, 1407
CciI	TCATGA	1 cut(s)	877
CfoI	GCGC	3 cut(s)	131, 1033, 1302
Cfr10I	RCCGGY	1 cut(s)	898
Cfr13I	GGNCC	7 cut(s)	82, 277, 289, 448, 648, 790, 896
ClaI	ATCGAT	1 cut(s)	687
CseI	GACGC	2 cut(s)	227, 1358
CsiI	ACCWGGT	1 cut(s)	291
Csp6I	GTAC	3 cut(s)	187, 864, 1270
CspAI	ACCGGT	1 cut(s)	898
CviAII	CATG	6 cut(s)	731, 878, 965, 1274, 1314, 1331
CviJI	RGCY	7 cut(s)	235, 634, 1123, 1130, 1252, 1297, 1385
CviKI_1	RGCY	7 cut(s)	235, 634, 1123, 1130, 1252, 1297, 1385
CviQI	GTAC	3 cut(s)	187, 864, 1270
DdeI	CTNAG	3 cut(s)	342, 1095, 1173
DinI	GGCGCC	1 cut(s)	1032
DraIII	CACNNNGTG	1 cut(s)	1205
Eam1104I	CTCTTC	2 cut(s)	182, 930
EarI	CTCTTC	2 cut(s)	182, 930
EciI	GGCGGA	2 cut(s)	26, 125
Eco130I	CCWWGG	2 cut(s)	730, 1273
Eco147I	AGGCCT	1 cut(s)	1123
Eco24I	GRGCYC	1 cut(s)	237
Eco31I	GGTCTC	1 cut(s)	1157
Eco47I	GGWCC	7 cut(s)	82, 277, 289, 448, 648, 790, 896
Eco57I	CTGAAG	2 cut(s)	396, 844
Eco88I	CYCGRG	1 cut(s)	524
Eco91I	GGTNACC	1 cut(s)	16
EcoO109I	RGGNCCY	1 cut(s)	82
EcoO65I	GGTNACC	1 cut(s)	16
EcoRI	GAATTC	2 cut(s)	694, 997
EcoRII	CCWGG	2 cut(s)	78, 291
EcoT14I	CCWWGG	2 cut(s)	730, 1273
EcoT38I	GRGCYC	1 cut(s)	237
EgeI	GGCGCC	1 cut(s)	1032
EheI	GGCGCC	1 cut(s)	1032
ErhI	CCWWGG	2 cut(s)	730, 1273
Esp3I	CGTCTC	1 cut(s)	345
FaeI	CATG	6 cut(s)	734, 881, 968, 1277, 1317, 1334
FaqI	GGGAC	4 cut(s)	178, 345, 521, 661
FatI	CATG	6 cut(s)	730, 877, 964, 1273, 1313, 1330
FauI	CCCGC	5 cut(s)	152, 318, 768, 1108, 1398
FbaI	TGATCA	1 cut(s)	874
FblI	GTMKAC	3 cut(s)	42, 159, 1327
Fnu4HI	GCNGC	7 cut(s)	283, 323, 764, 1131, 1250, 1298, 1353
FokI	GGATG	9 cut(s)	194, 515, 545, 674, 716, 743, 1144, 1193, 1232
FriOI	GRGCYC	1 cut(s)	237
Fsp4HI	GCNGC	7 cut(s)	283, 323, 764, 1131, 1250, 1298, 1353
FspI	TGCGCA	1 cut(s)	1301
GlaI	GCGC	3 cut(s)	130, 1032, 1301
GluI	GCNGC	7 cut(s)	283, 323, 764, 1131, 1250, 1298, 1353
HaeII	RGCGCY	1 cut(s)	1034
HaeIII	GGCC	1 cut(s)	1123
HapII	CCGG	6 cut(s)	243, 699, 899, 932, 1040, 1127
HgaI	GACGC	2 cut(s)	227, 1358
HhaI	GCGC	3 cut(s)	131, 1033, 1302
Hin1I	GRCGYC	3 cut(s)	39, 219, 1031
Hin1II	CATG	6 cut(s)	734, 881, 968, 1277, 1317, 1334
Hin6I	GCGC	3 cut(s)	129, 1031, 1300
HinP1I	GCGC	3 cut(s)	129, 1031, 1300
HincII	GTYRAC	3 cut(s)	43, 160, 1328
HindII	GTYRAC	3 cut(s)	43, 160, 1328
HinfI	GANTC	6 cut(s)	239, 416, 581, 653, 773, 890
HpaII	CCGG	6 cut(s)	243, 699, 899, 932, 1040, 1127
HphI	GGTGA	6 cut(s)	10, 145, 307, 587, 746, 1289
Hpy166II	GTNNAC	5 cut(s)	43, 160, 202, 349, 1328
Hpy188I	TCNGA	8 cut(s)	103, 124, 268, 376, 568, 658, 961, 1096
Hpy8I	GTNNAC	5 cut(s)	43, 160, 202, 349, 1328
HpyAV	CCTTC	3 cut(s)	31, 1142, 1362
HpyCH4III	ACNGT	2 cut(s)	157, 949
HpyCH4IV	ACGT	3 cut(s)	39, 204, 866
HpyCH4V	TGCA	7 cut(s)	718, 1064, 1109, 1199, 1215, 1260, 1284
HpyF10VI	GCNNNNNNNGC	8 cut(s)	135, 144, 331, 926, 1136, 1205, 1297, 1352
HpyF3I	CTNAG	3 cut(s)	342, 1095, 1173
HpySE526I	ACGT	3 cut(s)	39, 204, 866
Hsp92I	GRCGYC	3 cut(s)	39, 219, 1031
Hsp92II	CATG	6 cut(s)	734, 881, 968, 1277, 1317, 1334
HspAI	GCGC	3 cut(s)	129, 1031, 1300
KasI	GGCGCC	1 cut(s)	1030
Kpn2I	TCCGGA	2 cut(s)	242, 1039
KpnI	GGTACC	1 cut(s)	1273
Ksp22I	TGATCA	1 cut(s)	874
LmnI	GCTCC	1 cut(s)	232
Lsp1109I	GCAGC	6 cut(s)	334, 750, 1117, 1261, 1284, 1339
LweI	GCATC	3 cut(s)	156, 601, 765
MabI	ACCWGGT	1 cut(s)	291
MaeII	ACGT	3 cut(s)	39, 204, 866
MaeIII	GTNAC	2 cut(s)	16, 151
MboII	GAAGA	8 cut(s)	16, 169, 389, 576, 821, 845, 850, 947
MflI	RGATCY	3 cut(s)	245, 310, 956
MhlI	GDGCHC	2 cut(s)	76, 237
Mly113I	GGCGCC	1 cut(s)	1031
MlyI	GAGTC	1 cut(s)	425
MmeI	TCCRAC	2 cut(s)	52, 273
MroI	TCCGGA	2 cut(s)	242, 1039
MseI	TTAA	2 cut(s)	593, 1169
MspA1I	CMGCKG	4 cut(s)	325, 763, 1115, 1346
MspI	CCGG	6 cut(s)	243, 699, 899, 932, 1040, 1127
MspR9I	CCNGG	3 cut(s)	80, 293, 700
Mva1269I	GAATGC	1 cut(s)	1111
MvaI	CCWGG	2 cut(s)	80, 293
MvnI	CGCG	2 cut(s)	129, 285
MwoI	GCNNNNNNNGC	8 cut(s)	135, 144, 331, 926, 1136, 1205, 1297, 1352
NarI	GGCGCC	1 cut(s)	1031
NciI	CCSGG	1 cut(s)	700
NcoI	CCATGG	2 cut(s)	730, 1273
NlaIII	CATG	6 cut(s)	734, 881, 968, 1277, 1317, 1334
NlaIV	GGNNCC	7 cut(s)	234, 247, 649, 930, 1032, 1271, 1373
NmuCI	GTSAC	2 cut(s)	16, 151
NsbI	TGCGCA	1 cut(s)	1301
NspI	RCATGY	2 cut(s)	968, 1317
PagI	TCATGA	1 cut(s)	877
PceI	AGGCCT	1 cut(s)	1123
PciI	ACATGT	1 cut(s)	1313
PcsI	WCGNNNNNNNCGW	7 cut(s)	42, 45, 156, 168, 624, 813, 1356
PctI	GAATGC	1 cut(s)	1111
PfeI	GAWTC	5 cut(s)	239, 581, 653, 773, 890
PflMI	CCANNNNNTGG	1 cut(s)	1008
PinAI	ACCGGT	1 cut(s)	898
PkrI	GCNGC	7 cut(s)	284, 324, 765, 1132, 1251, 1299, 1354
Ple19I	CGATCG	2 cut(s)	127, 266
PleI	GAGTC	1 cut(s)	424
PluTI	GGCGCC	1 cut(s)	1034
PpsI	GAGTC	1 cut(s)	424
PpuMI	RGGWCCY	1 cut(s)	82
PscI	ACATGT	1 cut(s)	1313
Psp5II	RGGWCCY	1 cut(s)	82
Psp6I	CCWGG	2 cut(s)	78, 291
PspEI	GGTNACC	1 cut(s)	16
PspGI	CCWGG	2 cut(s)	78, 291
PspN4I	GGNNCC	7 cut(s)	234, 247, 649, 930, 1032, 1271, 1373
PspPI	GGNCC	7 cut(s)	82, 277, 289, 448, 648, 790, 896
PspPPI	RGGWCCY	1 cut(s)	82
PstI	CTGCAG	1 cut(s)	1066
PsuI	RGATCY	3 cut(s)	245, 310, 956
PvuI	CGATCG	2 cut(s)	127, 266
RsaI	GTAC	3 cut(s)	188, 865, 1271
RsaNI	GTAC	3 cut(s)	187, 864, 1270
SalI	GTCGAC	3 cut(s)	41, 158, 1326
SaqAI	TTAA	2 cut(s)	593, 1169
SatI	GCNGC	7 cut(s)	283, 323, 764, 1131, 1250, 1298, 1353
Sau96I	GGNCC	7 cut(s)	82, 277, 289, 448, 648, 790, 896
SchI	GAGTC	1 cut(s)	425
ScrFI	CCNGG	3 cut(s)	80, 293, 700
SduI	GDGCHC	2 cut(s)	76, 237
SexAI	ACCWGGT	1 cut(s)	291
SfaNI	GCATC	3 cut(s)	156, 601, 765
SfcI	CTRYAG	1 cut(s)	1062
SfoI	GGCGCC	1 cut(s)	1032
SgrDI	CGTCGACG	2 cut(s)	41, 158
SinI	GGWCC	7 cut(s)	82, 277, 289, 448, 648, 790, 896
SseBI	AGGCCT	1 cut(s)	1123
SspDI	GGCGCC	1 cut(s)	1030
StuI	AGGCCT	1 cut(s)	1123
StyD4I	CCNGG	3 cut(s)	78, 291, 698
StyI	CCWWGG	2 cut(s)	730, 1273
TaaI	ACNGT	2 cut(s)	157, 949
TaiI	ACGT	3 cut(s)	42, 207, 869
TaqII	GACCGA	1 cut(s)	665
TauI	GCSGC	1 cut(s)	285
TfiI	GAWTC	5 cut(s)	239, 581, 653, 773, 890
Tru1I	TTAA	2 cut(s)	593, 1169
Tru9I	TTAA	2 cut(s)	593, 1169
TscAI	CASTG	1 cut(s)	1066
TseFI	GTSAC	2 cut(s)	16, 151
TseI	GCWGC	6 cut(s)	322, 763, 1130, 1249, 1297, 1352
Tsp45I	GTSAC	2 cut(s)	16, 151
TspDTI	ATGAA	2 cut(s)	1241, 1347
TspGWI	ACGGA	1 cut(s)	1150
TspRI	CASTG	1 cut(s)	1066
Van91I	CCANNNNNTGG	1 cut(s)	1008
VpaK11BI	GGWCC	7 cut(s)	82, 277, 289, 448, 648, 790, 896
XapI	RAATTY	5 cut(s)	298, 569, 694, 987, 997
XceI	RCATGY	2 cut(s)	968, 1317
XmiI	GTMKAC	3 cut(s)	42, 159, 1327
ZraI	GACGTC	1 cut(s)	40

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.