Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0006229

Physical Location & Seq

Genomic Coordinates Forward (+)

34212448 .. 34217623

Gene Structure 5176 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1208000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1794 bp

ATGCCTACCGTCAGCGTCGGCCGCGATCGTCTGTATGCAGCTCTAGGAAAAACATACACTCAGGAGGAGTTCGAGGAGTTATGCTTCGACTTCGGCATCGAGCTCGACGACGTCACAACTGAGAAAGCAATTATTCGGAAGGAGAAACATTTGGATGATGAAGGCGGCAAAGGCGACGAAGACGAAGAAGTTATTTACAAAATCGAAGTTCCGGCGAATAGATACGATTTGCTTTGCCTCGAAGGGATTGCTCAATCGCTGCGTGTTTTCAATGGGCAGGAGGACATTCCAACATATAAGTTGGCAAACATTAGTAAGGAGTCGATGATTCAAATGCATGTGAAATCAGAGACTTCTTCCATTCGCCCCTTTGTTGTCTGTGCTGTTTTAAGAGGTGTAACATTTGATGAAGCGAGTTATAACAGCTTCATCGATCTCCAAGACAAGCTCCATCAAAATATCTGCCGGCGAAGAACCCTAGTTGCCATTGGGACTCATGACTTGGACACATTACAAGCCCCTTTCACATATGAAGCTTTGCCGCCTTCAAGCATAAATTTTGTGCCTTTGAAACAGGTGAAGAACTTCAGAGCAGATGAGCTTATGGAATTTTATAAATCGGATTTGAAGCTGAAGAAGTTCTTGCACATAATAGAGAACTCGCCTGTGTTTCCTGTTTTATATGATCACAAGAGAACTGTTTTGTCTTTACCGCCCATTATTAATGGTGCACATTCAGCAATCACATTAAAGACAAAGAGTGTTTTCGTAGAATGTACAGCCACTGATTTGACAAAAGCCAAGATTGTTCTGAACACAATAGTAACGGCATTTTCAGCATATTGTGAAAAGAAATTTGAGATTGAACCAGTTGAAGTGTTATATTCTGATGGAAGCTCACATGTTTATCCTGATTTATCCATCTACAACATGGATGTTTCTCTATCGTATGTCAATAGTTATGCTGGAGTATCTTTAGAAGCGAAAGAGGTTACTGGTCTGTTGAAGAGGATGCAATTGCATGCTGAGCAATCTAAATCTGACAATATTACTGTATCTGTACCTCCAACCAGAAGTGACATTCTTCACCCTTGTGATGTTGTGGAGGATGTTGCAATTGCTTATGGATACAATAATATTCCAATGAGAAAGCATATTCCAACGAGCGAGCCTGTTTCTTTGAAGCCACTAGCCTTGAATGAGTTCACTGAACTCATCAGATCAGAGATTGCAATGAATGGGTTTACAGAGGTCTTGACATTCATATTGGGCTCGTGGAAAGAAAACTTCCCCATGTTAAGATGTAAAGATGACAAATCAAAGGCTGTGGTCATTGGTAATCCCCGCTCATCTGATTGTGAGCTTGTCAGAACGAGTCTGATGCCTGGCATTTTGAAAACTGTTAATAACAACAAAGATCATCCGAAGCCCATAAAGATTTTCGAAGTGGGTGATATAGTACTATTGGATGAGAAGACAGATGTTGGTGCAAGAAATCGTTGCCAACTAGCAGCTCTGTACTGCGGTGCTACGTCTGGGTTAGAGTTGATACATGGCCTGGTGGACAGAATTATGGAAGCGGTTGGGGCTTCTTTTGTTCCTCTTGGTGACAATACAGGATACTTCATTGAGACTTCAGATGATCCCGAGTTTCTTCCGGGGAGACAAGCCAGCATCATCTATAAAGGGAAGCGCATTGGAATTTTTGGCATTTTACACCCTGAGGTGCTAACCAACTTTGACATTCCTGATCCGTGCTCCTATGTAGAACTTAACGTTGAGAGCTTGTTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006432 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009328 GO:0009987 GO:0010467 GO:0016070 GO:0019538 GO:0019752 GO:0032991 GO:0034641 GO:0034645 GO:0034660 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:1901360 GO:1901564 GO:1901566 GO:1901576 GO:1902494

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

B3_4 B5 tRNA-synt_2d

Protein Analysis

598

Amino Acids

66.78

Weight (kDa)

5.18

Isoelectric Point (pI)

42.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PhetRS_B1	PF18262	1 - 92	1.4e-31	Phe-tRNA synthetase beta subunit B1 domain
B3_4	PF03483	139 - 285	7.2e-11	B3/4 domain
B5	PF03484	311 - 377	5.8e-23	tRNA synthetase B5 domain
tRNA_synthFbeta	PF17759	386 - 593	6.3e-46	Phenylalanyl tRNA synthetase beta chain CLM domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0006229)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G72550 AT1G72550
fragaria_vesca	FvH4_5g13020 FvH4_5g13020 FvH4_5g13020 FvH4_5g13020 FvH4_5g13020 FvH4_5g13020
malus_domestica	MD06G1208000.v1.1 MD14G1218400.v1.1
prunus_persica	Prupe.5G041000_v2.0.a1 Prupe.5G212200_v2.0.a1
pyrus_communis	pycom12g20030
rosa_chinensis	RchiOBHm_Chr7g0184111
rosa_laevigata	RLG00000004999 RLG00000026882 RLG00000028559
rosa_multiflora	Rmu_sc0004712.1_g000004 Rmu_sc0025350.1_g000009
rosa_roxburghii	Rroxscaffold_3G00269870
rosa_rugosa	Rorug06G0463100
rosa_samantha	Rh7AG082700 Rh7BG064300 Rh7CG064900 Rh7DG064500
rosa_wichuraiana	Rw0G002490 Rw7G005290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	420, 615
AatII	GACGTC	1 cut(s)	114
AccBSI	CCGCTC	1 cut(s)	1347
AccII	CGCG	1 cut(s)	24
AciI	CCGC	7 cut(s)	22, 165, 542, 713, 1345, 1524, 1580
AclI	AACGTT	1 cut(s)	1776
AclWI	GGATC	2 cut(s)	1637, 1745
AcoI	YGGCCR	1 cut(s)	19
AcsI	RAATTY	4 cut(s)	556, 608, 854, 1701
AcuI	CTGAAG	3 cut(s)	571, 653, 1620
AcyI	GRCGYC	1 cut(s)	111
AfaI	GTAC	4 cut(s)	778, 1062, 1461, 1520
AflIII	ACRYGT	1 cut(s)	901
AjnI	CCWGG	2 cut(s)	1384, 1557
AleI	CACNNNNGTG	1 cut(s)	1092
Alw21I	GWGCWC	3 cut(s)	105, 733, 1760
Alw26I	GTCTC	3 cut(s)	344, 1625, 1657
Alw44I	GTGCAC	1 cut(s)	729
AlwI	GGATC	2 cut(s)	1637, 1745
AlwNI	CAGNNNCTG	1 cut(s)	785
Ama87I	CYCGRG	1 cut(s)	1646
AoxI	GGCC	2 cut(s)	19, 1555
ApaLI	GTGCAC	1 cut(s)	729
ApeKI	GCWGC	3 cut(s)	38, 259, 1511
ApoI	RAATTY	4 cut(s)	556, 608, 854, 1701
AseI	ATTAAT	1 cut(s)	723
Asp700I	GAANNNNTTC	3 cut(s)	584, 638, 1202
AspLEI	GCGC	1 cut(s)	1695
AsuC2I	CCSGG	1 cut(s)	1659
AsuHPI	GGTGA	4 cut(s)	589, 1079, 1463, 1619
AsuII	TTCGAA	1 cut(s)	1443
AvaI	CYCGRG	1 cut(s)	1646
AxyI	CCTNAGG	1 cut(s)	1722
BaeGI	GKGCMC	1 cut(s)	733
BanII	GRGCYC	2 cut(s)	105, 1274
BauI	CACGAG	1 cut(s)	1273
BbsI	GAAGAC	2 cut(s)	186, 1481
Bbv12I	GWGCWC	3 cut(s)	105, 733, 1760
BbvI	GCAGC	3 cut(s)	50, 246, 1523
BccI	CCATC	3 cut(s)	459, 884, 929
BceAI	ACGGC	1 cut(s)	843
BcgI	CGANNNNNNTGC	6 cut(s)	85, 119, 230, 264, 1363, 1397
BciT130I	CCWGG	2 cut(s)	1386, 1559
BciVI	GTATCC	2 cut(s)	1121, 1613
BclI	TGATCA	1 cut(s)	685
BcnI	CCSGG	1 cut(s)	1659
BcoDI	GTCTC	3 cut(s)	344, 1625, 1657
BfaI	CTAG	4 cut(s)	44, 479, 1190, 1508
BfuI	GTATCC	2 cut(s)	1121, 1613
BisI	GCNGC	6 cut(s)	22, 39, 166, 260, 542, 1512
BlpI	GCTNAGC	1 cut(s)	1026
BlsI	GCNGC	6 cut(s)	23, 40, 167, 261, 543, 1513
BmcAI	AGTACT	1 cut(s)	1461
Bme1390I	CCNGG	3 cut(s)	1386, 1559, 1659
BmeT110I	CYCGRG	1 cut(s)	1646
BmrFI	CCNGG	3 cut(s)	1386, 1559, 1659
BmsI	GCATC	4 cut(s)	105, 1002, 1371, 1683
BpiI	GAAGAC	2 cut(s)	186, 1481
BpmI	CTGGAG	1 cut(s)	987
Bpu1102I	GCTNAGC	1 cut(s)	1026
Bpu14I	TTCGAA	1 cut(s)	1443
BpuMI	CCSGG	1 cut(s)	1659
Bsa29I	ATCGAT	1 cut(s)	432
BsaHI	GRCGYC	1 cut(s)	111
BsaJI	CCNNGG	1 cut(s)	1658
Bse118I	RCCGGY	1 cut(s)	465
Bse1I	ACTGG	2 cut(s)	869, 1000
Bse21I	CCTNAGG	1 cut(s)	1722
Bse3DI	GCAATG	1 cut(s)	1239
BseBI	CCWGG	2 cut(s)	1386, 1559
BseCI	ATCGAT	1 cut(s)	432
BseDI	CCNNGG	1 cut(s)	1658
BseGI	GGATG	6 cut(s)	160, 940, 1017, 1114, 1420, 1474
BseMI	GCAATG	1 cut(s)	1239
BseMII	CTCAG	4 cut(s)	74, 111, 1017, 1713
BseNI	ACTGG	2 cut(s)	869, 1000
BseRI	GAGGAG	2 cut(s)	80, 89
BseSI	GKGCMC	1 cut(s)	733
BseX3I	CGGCCG	1 cut(s)	19
BseXI	GCAGC	3 cut(s)	50, 246, 1523
Bsh1236I	CGCG	1 cut(s)	24
Bsh1285I	CGRYCG	2 cut(s)	22, 28
BshFI	GGCC	2 cut(s)	21, 1557
BshVI	ATCGAT	1 cut(s)	432
BsiEI	CGRYCG	2 cut(s)	22, 28
BsiHKAI	GWGCWC	3 cut(s)	105, 733, 1760
BsiHKCI	CYCGRG	1 cut(s)	1646
BsiSI	CCGG	3 cut(s)	212, 466, 1658
BslFI	GGGAC	1 cut(s)	505
BsmAI	GTCTC	3 cut(s)	344, 1625, 1657
BsmFI	GGGAC	1 cut(s)	505
BsnI	GGCC	2 cut(s)	21, 1557
BsoBI	CYCGRG	1 cut(s)	1646
Bsp119I	TTCGAA	1 cut(s)	1443
Bsp1286I	GDGCHC	4 cut(s)	105, 733, 1274, 1760
Bsp1407I	TGTACA	1 cut(s)	776
Bsp143I	GATC	7 cut(s)	25, 433, 685, 1220, 1417, 1642, 1750
Bsp1720I	GCTNAGC	1 cut(s)	1026
BspACI	CCGC	7 cut(s)	22, 165, 542, 713, 1345, 1524, 1580
BspANI	GGCC	2 cut(s)	21, 1557
BspCNI	CTCAG	4 cut(s)	73, 112, 1018, 1714
BspDI	ATCGAT	1 cut(s)	432
BspFNI	CGCG	1 cut(s)	24
BspHI	TCATGA	1 cut(s)	496
BspPI	GGATC	2 cut(s)	1637, 1745
BspT104I	TTCGAA	1 cut(s)	1443
BsrBI	CCGCTC	1 cut(s)	1347
BsrDI	GCAATG	1 cut(s)	1239
BsrFI	RCCGGY	1 cut(s)	465
BsrGI	TGTACA	1 cut(s)	776
BsrI	ACTGG	2 cut(s)	869, 1000
BssAI	RCCGGY	1 cut(s)	465
BssECI	CCNNGG	1 cut(s)	1658
BssMI	GATC	7 cut(s)	25, 433, 685, 1220, 1417, 1642, 1750
BssNI	GRCGYC	1 cut(s)	111
BssSI	CACGAG	1 cut(s)	1273
Bst2BI	CACGAG	1 cut(s)	1273
Bst2UI	CCWGG	2 cut(s)	1386, 1559
Bst4CI	ACNGT	4 cut(s)	10, 700, 1054, 1402
Bst6I	CTCTTC	1 cut(s)	1001
BstACI	GRCGYC	1 cut(s)	111
BstAUI	TGTACA	1 cut(s)	776
BstBI	TTCGAA	1 cut(s)	1443
BstC8I	GCNNGC	4 cut(s)	467, 1023, 1169, 1672
BstDEI	CTNAG	4 cut(s)	60, 120, 1026, 1722
BstF5I	GGATG	6 cut(s)	160, 940, 1017, 1114, 1420, 1474
BstFNI	CGCG	1 cut(s)	24
BstHHI	GCGC	1 cut(s)	1695
BstKTI	GATC	7 cut(s)	28, 436, 688, 1223, 1420, 1645, 1753
BstMAI	GTCTC	3 cut(s)	344, 1625, 1657
BstMBI	GATC	7 cut(s)	25, 433, 685, 1220, 1417, 1642, 1750
BstMCI	CGRYCG	2 cut(s)	22, 28
BstMWI	GCNNNNNNNGC	6 cut(s)	21, 171, 737, 836, 1027, 1586
BstNI	CCWGG	2 cut(s)	1386, 1559
BstNSI	RCATGY	3 cut(s)	341, 905, 1025
BstSCI	CCNGG	3 cut(s)	1384, 1557, 1657
BstSLI	GKGCMC	1 cut(s)	733
BstUI	CGCG	1 cut(s)	24
BstV1I	GCAGC	3 cut(s)	50, 246, 1523
BstV2I	GAAGAC	2 cut(s)	186, 1481
BstZI	CGGCCG	1 cut(s)	19
Bsu15I	ATCGAT	1 cut(s)	432
Bsu36I	CCTNAGG	1 cut(s)	1722
BsuI	GTATCC	2 cut(s)	1121, 1613
BsuRI	GGCC	2 cut(s)	21, 1557
BsuTUI	ATCGAT	1 cut(s)	432
BtsCI	GGATG	6 cut(s)	160, 940, 1017, 1114, 1420, 1474
BtsIMutI	CAGTG	2 cut(s)	783, 1206
Cac8I	GCNNGC	4 cut(s)	467, 1023, 1169, 1672
CaiI	CAGNNNCTG	1 cut(s)	785
CciI	TCATGA	1 cut(s)	496
CfoI	GCGC	1 cut(s)	1695
Cfr10I	RCCGGY	1 cut(s)	465
ClaI	ATCGAT	1 cut(s)	432
CseI	GACGC	1 cut(s)	4
Csp6I	GTAC	4 cut(s)	777, 1061, 1460, 1519
CspCI	CAANNNNNGTGG	4 cut(s)	1176, 1211, 1308, 1343
CviAII	CATG	7 cut(s)	338, 497, 902, 931, 1022, 1294, 1553
CviQI	GTAC	4 cut(s)	777, 1061, 1460, 1519
DdeI	CTNAG	4 cut(s)	60, 120, 1026, 1722
DpnI	GATC	7 cut(s)	27, 435, 687, 1222, 1419, 1644, 1752
DpnII	GATC	7 cut(s)	25, 433, 685, 1220, 1417, 1642, 1750
EaeI	YGGCCR	1 cut(s)	19
EagI	CGGCCG	1 cut(s)	19
Eam1104I	CTCTTC	1 cut(s)	1001
EarI	CTCTTC	1 cut(s)	1001
Ecl136II	GAGCTC	1 cut(s)	103
EclXI	CGGCCG	1 cut(s)	19
Eco24I	GRGCYC	2 cut(s)	105, 1274
Eco52I	CGGCCG	1 cut(s)	19
Eco53kI	GAGCTC	1 cut(s)	103
Eco57I	CTGAAG	3 cut(s)	571, 653, 1620
Eco81I	CCTNAGG	1 cut(s)	1722
Eco88I	CYCGRG	1 cut(s)	1646
EcoICRI	GAGCTC	1 cut(s)	103
EcoRII	CCWGG	2 cut(s)	1384, 1557
EcoT22I	ATGCAT	1 cut(s)	339
EcoT38I	GRGCYC	2 cut(s)	105, 1274
FaeI	CATG	7 cut(s)	341, 500, 905, 934, 1025, 1297, 1556
FalI	AAGNNNNNCTT	2 cut(s)	626, 658
FaqI	GGGAC	1 cut(s)	505
FatI	CATG	7 cut(s)	337, 496, 901, 930, 1021, 1293, 1552
FauI	CCCGC	1 cut(s)	1352
FauNDI	CATATG	1 cut(s)	529
FbaI	TGATCA	1 cut(s)	685
Fnu4HI	GCNGC	6 cut(s)	22, 39, 166, 260, 542, 1512
FokI	GGATG	6 cut(s)	167, 947, 1024, 1121, 1407, 1481
FriOI	GRGCYC	2 cut(s)	105, 1274
Fsp4HI	GCNGC	6 cut(s)	22, 39, 166, 260, 542, 1512
FspBI	CTAG	4 cut(s)	44, 479, 1190, 1508
GlaI	GCGC	1 cut(s)	1694
GluI	GCNGC	6 cut(s)	22, 39, 166, 260, 542, 1512
GsuI	CTGGAG	1 cut(s)	987
HaeIII	GGCC	2 cut(s)	21, 1557
HapII	CCGG	3 cut(s)	212, 466, 1658
HgaI	GACGC	1 cut(s)	4
HhaI	GCGC	1 cut(s)	1695
Hin1I	GRCGYC	1 cut(s)	111
Hin1II	CATG	7 cut(s)	341, 500, 905, 934, 1025, 1297, 1556
Hin6I	GCGC	1 cut(s)	1693
HinP1I	GCGC	1 cut(s)	1693
HindIII	AAGCTT	1 cut(s)	534
HinfI	GANTC	4 cut(s)	320, 328, 493, 1375
HpaII	CCGG	3 cut(s)	212, 466, 1658
HphI	GGTGA	4 cut(s)	589, 1079, 1463, 1619
Hpy166II	GTNNAC	4 cut(s)	731, 1206, 1245, 1564
Hpy188III	TCNNGA	6 cut(s)	62, 497, 911, 1255, 1646, 1748
Hpy8I	GTNNAC	4 cut(s)	731, 1206, 1245, 1564
Hpy99I	CGWCG	4 cut(s)	20, 110, 113, 179
HpyAV	CCTTC	4 cut(s)	133, 155, 236, 555
HpyCH4III	ACNGT	4 cut(s)	10, 700, 1054, 1402
HpyCH4IV	ACGT	3 cut(s)	111, 1532, 1776
HpyCH4V	TGCA	9 cut(s)	38, 337, 646, 731, 1015, 1021, 1115, 1232, 1490
HpyF10VI	GCNNNNNNNGC	6 cut(s)	21, 171, 737, 836, 1027, 1586
HpyF3I	CTNAG	4 cut(s)	60, 120, 1026, 1722
HpySE526I	ACGT	3 cut(s)	111, 1532, 1776
Hsp92I	GRCGYC	1 cut(s)	111
Hsp92II	CATG	7 cut(s)	341, 500, 905, 934, 1025, 1297, 1556
HspAI	GCGC	1 cut(s)	1693
KroI	GCCGGC	1 cut(s)	465
KroNI	GCCGGC	1 cut(s)	467
Ksp22I	TGATCA	1 cut(s)	685
Kzo9I	GATC	7 cut(s)	25, 433, 685, 1220, 1417, 1642, 1750
LmnI	GCTCC	2 cut(s)	453, 1763
Lsp1109I	GCAGC	3 cut(s)	50, 246, 1523
LweI	GCATC	4 cut(s)	105, 1002, 1371, 1683
MaeI	CTAG	4 cut(s)	44, 479, 1190, 1508
MaeII	ACGT	3 cut(s)	111, 1532, 1776
MaeIII	GTNAC	6 cut(s)	112, 397, 823, 991, 1076, 1607
MalI	GATC	7 cut(s)	27, 435, 687, 1222, 1419, 1644, 1752
MbiI	CCGCTC	1 cut(s)	1347
MboI	GATC	7 cut(s)	25, 433, 685, 1220, 1417, 1642, 1750
MfeI	CAATTG	2 cut(s)	1016, 1116
MhlI	GDGCHC	4 cut(s)	105, 733, 1274, 1760
MluCI	AATT	8 cut(s)	129, 556, 608, 854, 1016, 1116, 1569, 1701
MlyI	GAGTC	3 cut(s)	329, 487, 1384
MmeI	TCCRAC	3 cut(s)	314, 1091, 1184
Mph1103I	ATGCAT	1 cut(s)	339
MroNI	GCCGGC	1 cut(s)	465
MroXI	GAANNNNTTC	3 cut(s)	584, 638, 1202
MseI	TTAA	6 cut(s)	389, 723, 749, 1298, 1404, 1773
MslI	CAYNNNNRTG	2 cut(s)	153, 1092
MspI	CCGG	3 cut(s)	212, 466, 1658
MspR9I	CCNGG	3 cut(s)	1386, 1559, 1659
MunI	CAATTG	2 cut(s)	1016, 1116
MvaI	CCWGG	2 cut(s)	1386, 1559
MvnI	CGCG	1 cut(s)	24
MwoI	GCNNNNNNNGC	6 cut(s)	21, 171, 737, 836, 1027, 1586
NaeI	GCCGGC	1 cut(s)	467
NciI	CCSGG	1 cut(s)	1659
NdeI	CATATG	1 cut(s)	529
NdeII	GATC	7 cut(s)	25, 433, 685, 1220, 1417, 1642, 1750
NgoMIV	GCCGGC	1 cut(s)	465
NlaIII	CATG	7 cut(s)	341, 500, 905, 934, 1025, 1297, 1556
NmuCI	GTSAC	3 cut(s)	112, 1076, 1607
NsiI	ATGCAT	1 cut(s)	339
NspI	RCATGY	3 cut(s)	341, 905, 1025
NspV	TTCGAA	1 cut(s)	1443
OliI	CACNNNNGTG	1 cut(s)	1092
PaeI	GCATGC	1 cut(s)	1025
PagI	TCATGA	1 cut(s)	496
PciI	ACATGT	1 cut(s)	901
PcsI	WCGNNNNNNNCGW	1 cut(s)	105
PdiI	GCCGGC	1 cut(s)	467
PdmI	GAANNNNTTC	3 cut(s)	584, 638, 1202
PfeI	GAWTC	1 cut(s)	328
PflFI	GACNNNGTC	1 cut(s)	110
PkrI	GCNGC	6 cut(s)	23, 40, 167, 261, 543, 1513
Ple19I	CGATCG	1 cut(s)	28
PleI	GAGTC	3 cut(s)	328, 487, 1383
PpsI	GAGTC	3 cut(s)	328, 487, 1383
PscI	ACATGT	1 cut(s)	901
PshBI	ATTAAT	1 cut(s)	723
PsiI	TTATAA	2 cut(s)	420, 615
Psp124BI	GAGCTC	1 cut(s)	105
Psp1406I	AACGTT	1 cut(s)	1776
Psp6I	CCWGG	2 cut(s)	1384, 1557
PspGI	CCWGG	2 cut(s)	1384, 1557
PstNI	CAGNNNCTG	1 cut(s)	785
PsyI	GACNNNGTC	1 cut(s)	110
PvuI	CGATCG	1 cut(s)	28
RsaI	GTAC	4 cut(s)	778, 1062, 1461, 1520
RsaNI	GTAC	4 cut(s)	777, 1061, 1460, 1519
RseI	CAYNNNNRTG	2 cut(s)	153, 1092
SacI	GAGCTC	1 cut(s)	105
SaqAI	TTAA	6 cut(s)	389, 723, 749, 1298, 1404, 1773
SatI	GCNGC	6 cut(s)	22, 39, 166, 260, 542, 1512
Sau3AI	GATC	7 cut(s)	25, 433, 685, 1220, 1417, 1642, 1750
ScaI	AGTACT	1 cut(s)	1461
SchI	GAGTC	3 cut(s)	329, 487, 1384
ScrFI	CCNGG	3 cut(s)	1386, 1559, 1659
SduI	GDGCHC	4 cut(s)	105, 733, 1274, 1760
SfaNI	GCATC	4 cut(s)	105, 1002, 1371, 1683
SfuI	TTCGAA	1 cut(s)	1443
SmiMI	CAYNNNNRTG	2 cut(s)	153, 1092
SphI	GCATGC	1 cut(s)	1025
Sse9I	AATT	8 cut(s)	129, 556, 608, 854, 1016, 1116, 1569, 1701
SsiI	CCGC	7 cut(s)	22, 165, 542, 713, 1345, 1524, 1580
SspI	AATATT	2 cut(s)	1048, 1138
SspMI	CTAG	4 cut(s)	44, 479, 1190, 1508
SstI	GAGCTC	1 cut(s)	105
StyD4I	CCNGG	3 cut(s)	1384, 1557, 1657
TaaI	ACNGT	4 cut(s)	10, 700, 1054, 1402
TaiI	ACGT	3 cut(s)	114, 1535, 1779
TaqI	TCGA	9 cut(s)	72, 87, 99, 105, 204, 240, 323, 432, 1443
TasI	AATT	8 cut(s)	129, 556, 608, 854, 1016, 1116, 1569, 1701
TatI	WGTACW	3 cut(s)	776, 1459, 1518
TauI	GCSGC	3 cut(s)	24, 168, 544
TfiI	GAWTC	1 cut(s)	328
Tru1I	TTAA	6 cut(s)	389, 723, 749, 1298, 1404, 1773
Tru9I	TTAA	6 cut(s)	389, 723, 749, 1298, 1404, 1773
TscAI	CASTG	2 cut(s)	790, 1213
TseFI	GTSAC	3 cut(s)	112, 1076, 1607
TseI	GCWGC	3 cut(s)	38, 259, 1511
Tsp45I	GTSAC	3 cut(s)	112, 1076, 1607
TspDTI	ATGAA	7 cut(s)	174, 418, 423, 546, 1250, 1252, 1615
TspGWI	ACGGA	1 cut(s)	1743
TspRI	CASTG	2 cut(s)	790, 1213
Tth111I	GACNNNGTC	1 cut(s)	110
VneI	GTGCAC	1 cut(s)	729
VspI	ATTAAT	1 cut(s)	723
XapI	RAATTY	4 cut(s)	556, 608, 854, 1701
XceI	RCATGY	3 cut(s)	341, 905, 1025
XcmI	CCANNNNNNNNNTGG	1 cut(s)	928
XmnI	GAANNNNTTC	3 cut(s)	584, 638, 1202
XspI	CTAG	4 cut(s)	44, 479, 1190, 1508
ZraI	GACGTC	1 cut(s)	112
ZrmI	AGTACT	1 cut(s)	1461
Zsp2I	ATGCAT	1 cut(s)	339

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.