Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0006229

Physical Location & Seq

Genomic Coordinates Forward (+)

30145851 .. 30150419

Gene Structure 4569 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1218400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1788 bp

ATGCCTACCGTCAGCGTCGGCCGCGATCGTCTGTATGCAGCTCTAGGAAAAACTTACACTCAGGAGCAGTTCGAGGAGTTATGCTTCGACTTCGGCATCGAGCTCGACGACGTCACAACTGAGAAGGCAATTATTCGGAAAGAGAAGAATTTGGATGATGATGAAGGCGGCGAAGACGAAGAAGTTATTTACAAAATCGAAGTTCCGGCGAATAGATACGATTTGCTTTGCCTCGAAGGGATTGCTCAATCGCTGCGTGTTTTCAATGGCCAAGAGGATAATCCTACGTATAAGTTGGCGAACATTAGTAAGGAGTCGATGATTAAAATGCATGTAAAACCAGAGACGTCTTTGATTCGCCCTCATATTGTCTGTGCCGTTTTAAGAGGTGTAGCATTCAATGAAGCTAGTTATAACAGCTTTATTGATCTCCAAGACAAGCTCCATCAAAATATCTGCCGGCGAAGAACCCTAGTTGCCATTGGGACTCATGACTTGGATACATTACAAGCCCCCTTCACATATGAGGCTTTGCCACCTTCAAGCATAAATTTTGTGCCTCTAAAACAGGTGAAGAACTTCAGAGCAGATGAGCTCATGGAATTTTATAAATCGGATTTGAAGCTGAAGAAGTTCTTGCACATAATAGAGAACTCACCAGTGTTTCCTGTTTTATATGATCACACCAGAACTGTTTTGTCTTTACCGCCCATTATTAATGGTGCACATTCAGCAATCACATTAAAGACAAAGAATGTGTTCATAGAATGTACAGCCACTGATTTGACAAAAGCCAAAATTGTTCTGAACACAATAGTAACAGCATTTTCAGCATATTGTGAAAGGAAATTTGAGATTGAACCAGTTGAAGTGTTATATTCTGATGGAAACTCACATGTTTATCCTGATTTATTGATCTACAACATGGATGTTTCTCTATCGTATGTCAATAGTTACGCTGGAGTGTCTTTGGAAGCAAAAGAGGTTACTGATCTGTTGAAGAGGATGCAATTGAATGCTGAGCAATCTAAATCTGACAATATTACTGTATCTGTACCTCCAACCAGAAGTGACATTCTTCACCCTTGTGATGTTGTGGAGGATGTTGCAATTGCTTATGGATACAATAATATTCCAATGAGAAAGCATATTCCAACGAGCGAGCCTGTTTCTTTGAAGCCACTAGCCTTGAATGAGTTCACTGAACTCATTAGATTAGAGATTGCAATGATTGGGTTTACAGAGGTCTTGACATTCGTATTGGGCTCGTGGAAAGAAAACTTCCCAATGTTAAGATGTAAAGATGACAAATCGAAGGCTGTGGTCATTGCAAATCCTCGCTCATCTGATTTTGAGCTTGTCAGAACCAGTCTGATGCCTGGCATTTTGAAAACTGTTAATAACAACAAAGATCATCCGAAGCCCATAAAGATTTTCGAAGTGGGTGATGTAGTACTGTTGGATGAGAAGACCGATGTTGGTGCAAGAAATCATTGCCAACTAGCAGCTCTATACTGTGGTGCTACATCGGGGTTAGAGCTGATACATGGCCTGGTGGACAGAATTATGGGAGCGGTTGGGGCTTCTTGGGTTCCTATTGGTGACAATACAGGATACTTCATCGAGACTTCAGATGATCCCGAGTTTCTTCCCGGGAGACAAGCAAGCATCATCTATAAAGGGAAGCACATTGGAATTTTTGGCATTTTACACCCCGAGGTGTTAAACAACTTTGACATTCCCGATCCGTGCTCCTATGTAAAACTTAACGTTGAGAGCTTGTTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006432 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009328 GO:0009987 GO:0010467 GO:0016070 GO:0019538 GO:0019752 GO:0032991 GO:0034641 GO:0034645 GO:0034660 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:1901360 GO:1901564 GO:1901566 GO:1901576 GO:1902494

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

B3_4 B5 tRNA-synt_2d

Protein Analysis

596

Amino Acids

66.71

Weight (kDa)

5.24

Isoelectric Point (pI)

40.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PhetRS_B1	PF18262	1 - 90	1.1e-31	Phe-tRNA synthetase beta subunit B1 domain
B3_4	PF03483	136 - 285	4.1e-12	B3/4 domain
B5	PF03484	309 - 375	1.8e-23	tRNA synthetase B5 domain
tRNA_synthFbeta	PF17759	384 - 590	2e-45	Phenylalanyl tRNA synthetase beta chain CLM domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0006229)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G72550 AT1G72550
fragaria_vesca	FvH4_5g13020 FvH4_5g13020 FvH4_5g13020 FvH4_5g13020 FvH4_5g13020 FvH4_5g13020
malus_domestica	MD06G1208000.v1.1 MD14G1218400.v1.1
prunus_persica	Prupe.5G041000_v2.0.a1 Prupe.5G212200_v2.0.a1
pyrus_communis	pycom12g20030
rosa_chinensis	RchiOBHm_Chr7g0184111
rosa_laevigata	RLG00000004999 RLG00000026882 RLG00000028559
rosa_multiflora	Rmu_sc0004712.1_g000004 Rmu_sc0025350.1_g000009
rosa_roxburghii	Rroxscaffold_3G00269870
rosa_rugosa	Rorug06G0463100
rosa_samantha	Rh7AG082700 Rh7BG064300 Rh7CG064900 Rh7DG064500
rosa_wichuraiana	Rw0G002490 Rw7G005290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	414, 609
AatII	GACGTC	2 cut(s)	114, 350
AccBSI	CCGCTC	1 cut(s)	1574
AccII	CGCG	1 cut(s)	24
AciI	CCGC	4 cut(s)	22, 168, 707, 1574
AclI	AACGTT	1 cut(s)	1770
AclWI	GGATC	2 cut(s)	1631, 1739
AcoI	YGGCCR	2 cut(s)	19, 268
AcsI	RAATTY	5 cut(s)	148, 550, 602, 848, 1695
AcuI	CTGAAG	3 cut(s)	565, 647, 1614
AcyI	GRCGYC	2 cut(s)	111, 347
AfaI	GTAC	3 cut(s)	772, 1056, 1455
AflIII	ACRYGT	1 cut(s)	895
AjnI	CCWGG	2 cut(s)	1378, 1551
AleI	CACNNNNGTG	1 cut(s)	1086
Alw21I	GWGCWC	4 cut(s)	105, 597, 727, 1754
Alw26I	GTCTC	3 cut(s)	338, 1619, 1651
Alw44I	GTGCAC	1 cut(s)	723
AlwI	GGATC	2 cut(s)	1631, 1739
AlwNI	CAGNNNCTG	1 cut(s)	779
Ama87I	CYCGRG	3 cut(s)	1640, 1652, 1715
AoxI	GGCC	3 cut(s)	19, 268, 1549
ApaLI	GTGCAC	1 cut(s)	723
ApeKI	GCWGC	3 cut(s)	38, 253, 1505
ApoI	RAATTY	5 cut(s)	148, 550, 602, 848, 1695
AseI	ATTAAT	1 cut(s)	717
Asp700I	GAANNNNTTC	4 cut(s)	578, 632, 758, 1196
AsuC2I	CCSGG	2 cut(s)	1653, 1654
AsuHPI	GGTGA	5 cut(s)	583, 648, 1073, 1457, 1613
AsuII	TTCGAA	1 cut(s)	1437
AvaI	CYCGRG	3 cut(s)	1640, 1652, 1715
BaeGI	GKGCMC	1 cut(s)	727
BalI	TGGCCA	1 cut(s)	270
BanII	GRGCYC	3 cut(s)	105, 597, 1268
BauI	CACGAG	1 cut(s)	1267
BbsI	GAAGAC	2 cut(s)	180, 1475
Bbv12I	GWGCWC	4 cut(s)	105, 597, 727, 1754
BbvI	GCAGC	3 cut(s)	50, 240, 1517
BccI	CCATC	2 cut(s)	453, 878
BceAI	ACGGC	1 cut(s)	362
BcgI	CGANNNNNNTGC	6 cut(s)	85, 119, 224, 258, 1463, 1497
BciT130I	CCWGG	2 cut(s)	1380, 1553
BciVI	GTATCC	3 cut(s)	493, 1115, 1607
BclI	TGATCA	1 cut(s)	679
BcnI	CCSGG	2 cut(s)	1653, 1654
BcoDI	GTCTC	3 cut(s)	338, 1619, 1651
BfaI	CTAG	5 cut(s)	44, 408, 473, 1184, 1502
BfuI	GTATCC	3 cut(s)	493, 1115, 1607
BisI	GCNGC	5 cut(s)	22, 39, 169, 254, 1506
BlpI	GCTNAGC	1 cut(s)	1020
BlsI	GCNGC	5 cut(s)	23, 40, 170, 255, 1507
BmcAI	AGTACT	1 cut(s)	1455
Bme1390I	CCNGG	4 cut(s)	1380, 1553, 1653, 1654
BmeT110I	CYCGRG	3 cut(s)	1640, 1652, 1715
BmiI	GGNNCC	1 cut(s)	1593
BmrFI	CCNGG	4 cut(s)	1380, 1553, 1653, 1654
BmsI	GCATC	4 cut(s)	105, 996, 1365, 1677
BpiI	GAAGAC	2 cut(s)	180, 1475
BpmI	CTGGAG	1 cut(s)	981
Bpu1102I	GCTNAGC	1 cut(s)	1020
Bpu14I	TTCGAA	1 cut(s)	1437
BpuMI	CCSGG	2 cut(s)	1653, 1654
BsaAI	YACGTR	1 cut(s)	288
BsaHI	GRCGYC	2 cut(s)	111, 347
BsaJI	CCNNGG	2 cut(s)	1652, 1716
Bse118I	RCCGGY	1 cut(s)	459
Bse1I	ACTGG	3 cut(s)	659, 863, 1368
Bse3DI	GCAATG	3 cut(s)	1233, 1326, 1492
BseBI	CCWGG	2 cut(s)	1380, 1553
BseDI	CCNNGG	2 cut(s)	1652, 1716
BseGI	GGATG	6 cut(s)	160, 934, 1011, 1108, 1414, 1468
BseMI	GCAATG	3 cut(s)	1233, 1326, 1492
BseMII	CTCAG	3 cut(s)	74, 111, 1011
BseNI	ACTGG	3 cut(s)	659, 863, 1368
BseRI	GAGGAG	1 cut(s)	89
BseSI	GKGCMC	1 cut(s)	727
BseX3I	CGGCCG	1 cut(s)	19
BseXI	GCAGC	3 cut(s)	50, 240, 1517
Bsh1236I	CGCG	1 cut(s)	24
Bsh1285I	CGRYCG	2 cut(s)	22, 28
BshFI	GGCC	3 cut(s)	21, 270, 1551
BsiEI	CGRYCG	2 cut(s)	22, 28
BsiHKAI	GWGCWC	4 cut(s)	105, 597, 727, 1754
BsiHKCI	CYCGRG	3 cut(s)	1640, 1652, 1715
BsiSI	CCGG	3 cut(s)	206, 460, 1653
BslFI	GGGAC	1 cut(s)	499
BsmAI	GTCTC	3 cut(s)	338, 1619, 1651
BsmBI	CGTCTC	1 cut(s)	338
BsmFI	GGGAC	1 cut(s)	499
BsmI	GAATGC	2 cut(s)	395, 1021
BsnI	GGCC	3 cut(s)	21, 270, 1551
BsoBI	CYCGRG	3 cut(s)	1640, 1652, 1715
Bsp119I	TTCGAA	1 cut(s)	1437
Bsp1286I	GDGCHC	5 cut(s)	105, 597, 727, 1268, 1754
Bsp1407I	TGTACA	1 cut(s)	770
Bsp143I	GATC	8 cut(s)	25, 427, 679, 915, 991, 1411, 1636, 1744
Bsp1720I	GCTNAGC	1 cut(s)	1020
BspACI	CCGC	4 cut(s)	22, 168, 707, 1574
BspANI	GGCC	3 cut(s)	21, 270, 1551
BspCNI	CTCAG	3 cut(s)	73, 112, 1012
BspFNI	CGCG	1 cut(s)	24
BspHI	TCATGA	1 cut(s)	490
BspLI	GGNNCC	1 cut(s)	1593
BspPI	GGATC	2 cut(s)	1631, 1739
BspT104I	TTCGAA	1 cut(s)	1437
BsrBI	CCGCTC	1 cut(s)	1574
BsrDI	GCAATG	3 cut(s)	1233, 1326, 1492
BsrFI	RCCGGY	1 cut(s)	459
BsrGI	TGTACA	1 cut(s)	770
BsrI	ACTGG	3 cut(s)	659, 863, 1368
BssAI	RCCGGY	1 cut(s)	459
BssECI	CCNNGG	2 cut(s)	1652, 1716
BssMI	GATC	8 cut(s)	25, 427, 679, 915, 991, 1411, 1636, 1744
BssNI	GRCGYC	2 cut(s)	111, 347
BssSI	CACGAG	1 cut(s)	1267
Bst2BI	CACGAG	1 cut(s)	1267
Bst2UI	CCWGG	2 cut(s)	1380, 1553
Bst4CI	ACNGT	6 cut(s)	10, 694, 1048, 1396, 1458, 1517
Bst6I	CTCTTC	1 cut(s)	995
BstACI	GRCGYC	2 cut(s)	111, 347
BstAUI	TGTACA	1 cut(s)	770
BstBAI	YACGTR	1 cut(s)	288
BstBI	TTCGAA	1 cut(s)	1437
BstC8I	GCNNGC	3 cut(s)	461, 1163, 1666
BstDEI	CTNAG	3 cut(s)	60, 120, 1020
BstF5I	GGATG	6 cut(s)	160, 934, 1011, 1108, 1414, 1468
BstFNI	CGCG	1 cut(s)	24
BstKTI	GATC	8 cut(s)	28, 430, 682, 918, 994, 1414, 1639, 1747
BstMAI	GTCTC	3 cut(s)	338, 1619, 1651
BstMBI	GATC	8 cut(s)	25, 427, 679, 915, 991, 1411, 1636, 1744
BstMCI	CGRYCG	2 cut(s)	22, 28
BstMWI	GCNNNNNNNGC	4 cut(s)	21, 731, 830, 1580
BstNI	CCWGG	2 cut(s)	1380, 1553
BstNSI	RCATGY	2 cut(s)	335, 899
BstSCI	CCNGG	4 cut(s)	1378, 1551, 1651, 1652
BstSLI	GKGCMC	1 cut(s)	727
BstSNI	TACGTA	1 cut(s)	288
BstUI	CGCG	1 cut(s)	24
BstV1I	GCAGC	3 cut(s)	50, 240, 1517
BstV2I	GAAGAC	2 cut(s)	180, 1475
BstZI	CGGCCG	1 cut(s)	19
BsuI	GTATCC	3 cut(s)	493, 1115, 1607
BsuRI	GGCC	3 cut(s)	21, 270, 1551
BtsCI	GGATG	6 cut(s)	160, 934, 1011, 1108, 1414, 1468
BtsIMutI	CAGTG	3 cut(s)	666, 777, 1200
Cac8I	GCNNGC	3 cut(s)	461, 1163, 1666
CaiI	CAGNNNCTG	1 cut(s)	779
CciI	TCATGA	1 cut(s)	490
Cfr10I	RCCGGY	1 cut(s)	459
Cfr9I	CCCGGG	1 cut(s)	1652
CseI	GACGC	1 cut(s)	4
Csp6I	GTAC	3 cut(s)	771, 1055, 1454
CspCI	CAANNNNNGTGG	2 cut(s)	1170, 1205
CviAII	CATG	6 cut(s)	332, 491, 598, 896, 925, 1547
CviQI	GTAC	3 cut(s)	771, 1055, 1454
DdeI	CTNAG	3 cut(s)	60, 120, 1020
DpnI	GATC	8 cut(s)	27, 429, 681, 917, 993, 1413, 1638, 1746
DpnII	GATC	8 cut(s)	25, 427, 679, 915, 991, 1411, 1636, 1744
EaeI	YGGCCR	2 cut(s)	19, 268
EagI	CGGCCG	1 cut(s)	19
Eam1104I	CTCTTC	1 cut(s)	995
EarI	CTCTTC	1 cut(s)	995
Ecl136II	GAGCTC	2 cut(s)	103, 595
EclXI	CGGCCG	1 cut(s)	19
Eco105I	TACGTA	1 cut(s)	288
Eco24I	GRGCYC	3 cut(s)	105, 597, 1268
Eco52I	CGGCCG	1 cut(s)	19
Eco53kI	GAGCTC	2 cut(s)	103, 595
Eco57I	CTGAAG	3 cut(s)	565, 647, 1614
Eco88I	CYCGRG	3 cut(s)	1640, 1652, 1715
EcoICRI	GAGCTC	2 cut(s)	103, 595
EcoRII	CCWGG	2 cut(s)	1378, 1551
EcoT22I	ATGCAT	1 cut(s)	333
EcoT38I	GRGCYC	3 cut(s)	105, 597, 1268
Esp3I	CGTCTC	1 cut(s)	338
FaeI	CATG	6 cut(s)	335, 494, 601, 899, 928, 1550
FalI	AAGNNNNNCTT	2 cut(s)	620, 652
FaqI	GGGAC	1 cut(s)	499
FatI	CATG	6 cut(s)	331, 490, 597, 895, 924, 1546
FauNDI	CATATG	1 cut(s)	523
FbaI	TGATCA	1 cut(s)	679
Fnu4HI	GCNGC	5 cut(s)	22, 39, 169, 254, 1506
FokI	GGATG	6 cut(s)	167, 941, 1018, 1115, 1401, 1475
FriOI	GRGCYC	3 cut(s)	105, 597, 1268
Fsp4HI	GCNGC	5 cut(s)	22, 39, 169, 254, 1506
FspBI	CTAG	5 cut(s)	44, 408, 473, 1184, 1502
GluI	GCNGC	5 cut(s)	22, 39, 169, 254, 1506
GsuI	CTGGAG	1 cut(s)	981
HaeIII	GGCC	3 cut(s)	21, 270, 1551
HapII	CCGG	3 cut(s)	206, 460, 1653
HgaI	GACGC	1 cut(s)	4
Hin1I	GRCGYC	2 cut(s)	111, 347
Hin1II	CATG	6 cut(s)	335, 494, 601, 899, 928, 1550
HinfI	GANTC	3 cut(s)	314, 355, 487
HpaII	CCGG	3 cut(s)	206, 460, 1653
HphI	GGTGA	5 cut(s)	583, 648, 1073, 1457, 1613
Hpy166II	GTNNAC	4 cut(s)	725, 1200, 1239, 1558
Hpy188III	TCNNGA	7 cut(s)	62, 491, 905, 1249, 1624, 1640, 1742
Hpy8I	GTNNAC	4 cut(s)	725, 1200, 1239, 1558
Hpy99I	CGWCG	3 cut(s)	20, 110, 113
HpyAV	CCTTC	6 cut(s)	118, 158, 230, 526, 549, 1309
HpyCH4III	ACNGT	6 cut(s)	10, 694, 1048, 1396, 1458, 1517
HpyCH4IV	ACGT	4 cut(s)	111, 287, 347, 1770
HpyCH4V	TGCA	9 cut(s)	38, 331, 640, 725, 1009, 1109, 1226, 1331, 1484
HpyF10VI	GCNNNNNNNGC	4 cut(s)	21, 731, 830, 1580
HpyF3I	CTNAG	3 cut(s)	60, 120, 1020
HpySE526I	ACGT	4 cut(s)	111, 287, 347, 1770
Hsp92I	GRCGYC	2 cut(s)	111, 347
Hsp92II	CATG	6 cut(s)	335, 494, 601, 899, 928, 1550
KroI	GCCGGC	1 cut(s)	459
KroNI	GCCGGC	1 cut(s)	461
Ksp22I	TGATCA	1 cut(s)	679
Kzo9I	GATC	8 cut(s)	25, 427, 679, 915, 991, 1411, 1636, 1744
LmnI	GCTCC	4 cut(s)	64, 447, 1571, 1757
Lsp1109I	GCAGC	3 cut(s)	50, 240, 1517
LweI	GCATC	4 cut(s)	105, 996, 1365, 1677
MaeI	CTAG	5 cut(s)	44, 408, 473, 1184, 1502
MaeII	ACGT	4 cut(s)	111, 287, 347, 1770
MaeIII	GTNAC	6 cut(s)	112, 817, 953, 985, 1070, 1601
MalI	GATC	8 cut(s)	27, 429, 681, 917, 993, 1413, 1638, 1746
MbiI	CCGCTC	1 cut(s)	1574
MboI	GATC	8 cut(s)	25, 427, 679, 915, 991, 1411, 1636, 1744
MfeI	CAATTG	2 cut(s)	1010, 1110
MhlI	GDGCHC	5 cut(s)	105, 597, 727, 1268, 1754
MlsI	TGGCCA	1 cut(s)	270
MluNI	TGGCCA	1 cut(s)	270
MlyI	GAGTC	2 cut(s)	323, 481
MmeI	TCCRAC	3 cut(s)	1085, 1178, 1440
Mox20I	TGGCCA	1 cut(s)	270
Mph1103I	ATGCAT	1 cut(s)	333
MroNI	GCCGGC	1 cut(s)	459
MroXI	GAANNNNTTC	4 cut(s)	578, 632, 758, 1196
MscI	TGGCCA	1 cut(s)	270
MseI	TTAA	8 cut(s)	324, 383, 717, 743, 1292, 1398, 1724, 1767
MslI	CAYNNNNRTG	1 cut(s)	1086
Msp20I	TGGCCA	1 cut(s)	270
MspI	CCGG	3 cut(s)	206, 460, 1653
MspR9I	CCNGG	4 cut(s)	1380, 1553, 1653, 1654
MunI	CAATTG	2 cut(s)	1010, 1110
Mva1269I	GAATGC	2 cut(s)	395, 1021
MvaI	CCWGG	2 cut(s)	1380, 1553
MvnI	CGCG	1 cut(s)	24
MwoI	GCNNNNNNNGC	4 cut(s)	21, 731, 830, 1580
NaeI	GCCGGC	1 cut(s)	461
NciI	CCSGG	2 cut(s)	1653, 1654
NdeI	CATATG	1 cut(s)	523
NdeII	GATC	8 cut(s)	25, 427, 679, 915, 991, 1411, 1636, 1744
NgoMIV	GCCGGC	1 cut(s)	459
NlaIII	CATG	6 cut(s)	335, 494, 601, 899, 928, 1550
NlaIV	GGNNCC	1 cut(s)	1593
NmuCI	GTSAC	3 cut(s)	112, 1070, 1601
NsiI	ATGCAT	1 cut(s)	333
NspI	RCATGY	2 cut(s)	335, 899
NspV	TTCGAA	1 cut(s)	1437
OliI	CACNNNNGTG	1 cut(s)	1086
PagI	TCATGA	1 cut(s)	490
PciI	ACATGT	1 cut(s)	895
PcsI	WCGNNNNNNNCGW	1 cut(s)	105
PctI	GAATGC	2 cut(s)	395, 1021
PdiI	GCCGGC	1 cut(s)	461
PdmI	GAANNNNTTC	4 cut(s)	578, 632, 758, 1196
PfeI	GAWTC	1 cut(s)	355
PflFI	GACNNNGTC	1 cut(s)	110
PkrI	GCNGC	5 cut(s)	23, 40, 170, 255, 1507
Ple19I	CGATCG	1 cut(s)	28
PleI	GAGTC	2 cut(s)	322, 481
PpsI	GAGTC	2 cut(s)	322, 481
Ppu21I	YACGTR	1 cut(s)	288
PscI	ACATGT	1 cut(s)	895
PshBI	ATTAAT	1 cut(s)	717
PsiI	TTATAA	2 cut(s)	414, 609
Psp124BI	GAGCTC	2 cut(s)	105, 597
Psp1406I	AACGTT	1 cut(s)	1770
Psp6I	CCWGG	2 cut(s)	1378, 1551
PspGI	CCWGG	2 cut(s)	1378, 1551
PspN4I	GGNNCC	1 cut(s)	1593
PstNI	CAGNNNCTG	1 cut(s)	779
PsyI	GACNNNGTC	1 cut(s)	110
PvuI	CGATCG	1 cut(s)	28
RsaI	GTAC	3 cut(s)	772, 1056, 1455
RsaNI	GTAC	3 cut(s)	771, 1055, 1454
RseI	CAYNNNNRTG	1 cut(s)	1086
SacI	GAGCTC	2 cut(s)	105, 597
SaqAI	TTAA	8 cut(s)	324, 383, 717, 743, 1292, 1398, 1724, 1767
SatI	GCNGC	5 cut(s)	22, 39, 169, 254, 1506
Sau3AI	GATC	8 cut(s)	25, 427, 679, 915, 991, 1411, 1636, 1744
ScaI	AGTACT	1 cut(s)	1455
SchI	GAGTC	2 cut(s)	323, 481
ScrFI	CCNGG	4 cut(s)	1380, 1553, 1653, 1654
SduI	GDGCHC	5 cut(s)	105, 597, 727, 1268, 1754
SfaNI	GCATC	4 cut(s)	105, 996, 1365, 1677
SfuI	TTCGAA	1 cut(s)	1437
SmaI	CCCGGG	1 cut(s)	1654
SmiMI	CAYNNNNRTG	1 cut(s)	1086
SnaBI	TACGTA	1 cut(s)	288
SsiI	CCGC	4 cut(s)	22, 168, 707, 1574
SspI	AATATT	2 cut(s)	1042, 1132
SspMI	CTAG	5 cut(s)	44, 408, 473, 1184, 1502
SstI	GAGCTC	2 cut(s)	105, 597
StyD4I	CCNGG	4 cut(s)	1378, 1551, 1651, 1652
TaaI	ACNGT	6 cut(s)	10, 694, 1048, 1396, 1458, 1517
TaiI	ACGT	4 cut(s)	114, 290, 350, 1773
TaqII	GACCGA	1 cut(s)	1487
TatI	WGTACW	2 cut(s)	770, 1453
TauI	GCSGC	2 cut(s)	24, 171
TfiI	GAWTC	1 cut(s)	355
Tru1I	TTAA	8 cut(s)	324, 383, 717, 743, 1292, 1398, 1724, 1767
Tru9I	TTAA	8 cut(s)	324, 383, 717, 743, 1292, 1398, 1724, 1767
TscAI	CASTG	3 cut(s)	666, 784, 1207
TseFI	GTSAC	3 cut(s)	112, 1070, 1601
TseI	GCWGC	3 cut(s)	38, 253, 1505
Tsp45I	GTSAC	3 cut(s)	112, 1070, 1601
TspDTI	ATGAA	4 cut(s)	177, 417, 751, 1609
TspGWI	ACGGA	1 cut(s)	1737
TspMI	CCCGGG	1 cut(s)	1652
TspRI	CASTG	3 cut(s)	666, 784, 1207
Tth111I	GACNNNGTC	1 cut(s)	110
VneI	GTGCAC	1 cut(s)	723
VspI	ATTAAT	1 cut(s)	717
XapI	RAATTY	5 cut(s)	148, 550, 602, 848, 1695
XceI	RCATGY	2 cut(s)	335, 899
XmaI	CCCGGG	1 cut(s)	1652
XmnI	GAANNNNTTC	4 cut(s)	578, 632, 758, 1196
XspI	CTAG	5 cut(s)	44, 408, 473, 1184, 1502
ZraI	GACGTC	2 cut(s)	112, 348
ZrmI	AGTACT	1 cut(s)	1455
Zsp2I	ATGCAT	1 cut(s)	333

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.