Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0006229

Physical Location & Seq

Genomic Coordinates Reverse (-)

64284416 .. 64290435

Gene Structure 6020 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000004999

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2220 bp

ATGCCGACCGTCAGCGTAGGACGGGATCGTCTGTTCGCCGCCCTAGGCAAAAGTTACACTAAGGAAGAGCTGAAGAAGAAGGAAGTGAGTGAGAAAGCTGAGAAAGAGTTTGAAGATGAGTGCTTCCGTTTCGGAATTGAGCTAGACGACGTCACAACTGAGAAAGCAATTATAAGGAAAGAAAAGCATCTGGATGAAGAAGAAGGTGACGAAGATGAGGAAGTTATATACAAAATCGACATTCCTGCTAATAGGTACGATTTGCTTTGCCTTGAAGGGCTTTCTCAAGCTCTCCGTATTTTCGAAAAGAAAGATGAGGTTCCTCAATATAAGTTGGCAAATGTTAGCAAGGAGTCTATGATGAAAATGCACGTGAAACCAGAGACATCTTTGATTCGACCTCATGTCGTTTGTGCTGTGTTAAGAGGGATGACTTTCACCGAAGCAAGCTATAACAGCTTTATTGACCTCCAAGACAAGCTCCATCAAAATATCTGCCGGCGACGAACCCTAGTTGCCATTGGGACTCATGACTTGGACACAATTCAAGCTCCTTTCTCATATGAGGCTTTGCCACCCTCAAGCATAAGTTTTGTGCCACTGAAACAGGTGAAGAACTTCAGAGCTGATGAACTCATGGAATTTTACAAATCAGATTTGAAGCTGAAGAAGTTCTTGCATATAATAGAGAACTCCAAGGTTTTTCCTGTTATATATGATAACAACAGAACTGTTGTGTCGTTACCTCCAATTATAAATGGTGCTCATTCAGCAATCACTTTAAAGACGAAGAATGTTTTCATAGAATGTACAGCTACTGATTTGACAAAGGCCAAGATTGTTCTGAACACAATAGTGACAGCATTTTCATCATATTGTGAAAGAAAGTTTGAGATTGAACCTGTTGAAGTGTTATATCCTGATGGAAAGTCATTTGTGTATCCTGATTTATCAATCTACAACATGGACGTTTCGCTATCATACATTAATCGCAAAATTGGACTATCTTTGGGGGCTGATGAGGTCATTGATTTATTGAATAAGATGCAATTGGGGGCTAAGCAGTCTGTATCAGGCAATAATCAGGACGATATCACTGTACTTATACCTCCAACAAGAAGTGACATCCTTCACCCTTGTGATGTCATGGAGGATGTTGCAATTGCATATGGATACAACAATATTCCGCGCACGAAGCCTGCATCTTTGCCTGGATCTTTGAAGCCACTTGTCTTGAATGAGCTTAGCGATGTTCTCAGATCTGAGATTGCAATGAATGGGTATACAGAGGTGTTGAATTTCATATTGTGCTCGTGGCGAGAAAACTTTGACATGTTACGTCGTAGAGATGACAAATCAAAGGCTGTTGTTGTTGGCAATCCTCGCTCATCTGATTTTGAGCTTGTGCGAACCACTCTGATGCCTGGCATATTGAAATCCGTGGGTCACAACAAAGATCACCCAAAGCCCATAAAGGTTTTCGAAGTGGGTGATGTGACTATGTTGGATGAGAAAAAAGATGTCGGTGCAACGAACCGTCGCCAATTAGCTGCATTATACTGTGGGGCTAGCTCTGGATTTGAGTTGATTCATGGGCTGGTGGATAGAATCATGGAAGTACTTGGGGTTGCTTTTGTTCCTAGTGGTGACAATGCAGGCTACTATTTACAACATGCAGAGGAGCCTGAGTTTCTGCCTGGTGTACAAGCAAGCATCTATTATAAAGGGAAGCACATTGGAAATTTTGGTATTTTGCACCCTGAGGTCTTAAAGAACTTTGATATTCCTGATCCTTGCTCATATGTGGAAATGAACATTGAGAGTTTCTTGCGGAACGATGGAAGTGGAGCCGGTTTTTCGGGTCTGAGCGGAGCGGTCGACGGCAGTTTCGCTTTTGATGGACTAGTGACTCGGATCTCTGGTGTTAGGGGCGGTCTCGGCTCTCTGGTGGTCGTCGGCGCTCTGCGATTGGGGTTCGGCGTACATGAGTTCAATGTTGGTCGGCCTCTGCTCGCCGGCGTCGAAGCCTTCCTTCATCCTCCGGTGCTGGTTTCAGGTCCAGCTCGTCGGCGTCGAGAGCAGGAGCCTCTTGGAGGATTCCGGTACGGGTCATGTGGCTTCAAGAGTTGGGTTTGTTGGGTCGCTGGCATCCTGTCGTCCTCTGTGGTACAGGATGCTGATGATGACGAGAAATTCGATGAGGACGAAGTTGATGAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006432 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009328 GO:0009987 GO:0010467 GO:0016070 GO:0019538 GO:0019752 GO:0032991 GO:0034641 GO:0034645 GO:0034660 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:1901360 GO:1901564 GO:1901566 GO:1901576 GO:1902494

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

B3_4 B5 tRNA-synt_2d

Protein Analysis

740

Amino Acids

82.36

Weight (kDa)

5.35

Isoelectric Point (pI)

42.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PhetRS_B1	PF18262	1 - 104	6.8e-26	Phe-tRNA synthetase beta subunit B1 domain
B3_4	PF03483	150 - 298	3.4e-13	B3/4 domain
B5	PF03484	322 - 392	1.2e-20	tRNA synthetase B5 domain
tRNA_synthFbeta	PF17759	395 - 606	4e-48	Phenylalanyl tRNA synthetase beta chain CLM domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006229)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G72550 AT1G72550
fragaria_vesca	FvH4_5g13020 FvH4_5g13020 FvH4_5g13020 FvH4_5g13020 FvH4_5g13020 FvH4_5g13020
malus_domestica	MD06G1208000.v1.1 MD14G1218400.v1.1
prunus_persica	Prupe.5G041000_v2.0.a1 Prupe.5G212200_v2.0.a1
pyrus_communis	pycom12g20030
rosa_chinensis	RchiOBHm_Chr7g0184111
rosa_laevigata	RLG00000004999 RLG00000026882 RLG00000028559
rosa_multiflora	Rmu_sc0004712.1_g000004 Rmu_sc0025350.1_g000009
rosa_roxburghii	Rroxscaffold_3G00269870
rosa_rugosa	Rorug06G0463100
rosa_samantha	Rh7AG082700 Rh7BG064300 Rh7CG064900 Rh7DG064500
rosa_wichuraiana	Rw0G002490 Rw7G005290

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	173, 755, 1722
AasI	GACNNNNNNGTC	1 cut(s)	27
AatII	GACGTC	1 cut(s)	153
AccBSI	CCGCTC	2 cut(s)	1869, 1874
AccI	GTMKAC	2 cut(s)	1283, 1878
AccII	CGCG	1 cut(s)	1189
AciI	CCGC	6 cut(s)	39, 1187, 1831, 1869, 1874, 1932
AclWI	GGATC	4 cut(s)	33, 1222, 1784, 1922
AcsI	RAATTY	4 cut(s)	641, 1297, 1741, 2192
AcuI	CTGAAG	3 cut(s)	92, 604, 686
AcvI	CACGTG	1 cut(s)	373
AcyI	GRCGYC	3 cut(s)	150, 2019, 2071
AfaI	GTAC	8 cut(s)	257, 811, 1101, 1620, 1704, 1983, 2105, 2169
AfiI	CCNNNNNNNGG	1 cut(s)	2093
AflIII	ACRYGT	1 cut(s)	1332
AhdI	GACNNNNNGTC	1 cut(s)	404
AhlI	ACTAGT	1 cut(s)	1903
AjnI	CCWGG	3 cut(s)	1210, 1423, 1696
AleI	CACNNNNGTG	2 cut(s)	854, 1137
AloI	GAACNNNNNNTCC	2 cut(s)	17, 49
Alw21I	GWGCWC	2 cut(s)	766, 1313
Alw26I	GTCTC	2 cut(s)	377, 1940
AlwI	GGATC	4 cut(s)	33, 1222, 1784, 1922
AlwNI	CAGNNNCTG	1 cut(s)	818
AoxI	GGCC	2 cut(s)	831, 2003
ApeKI	GCWGC	1 cut(s)	1550
ApoI	RAATTY	4 cut(s)	641, 1297, 1741, 2192
ArsI	GACNNNNNNTTYG	4 cut(s)	1871, 1903, 2177, 2209
AseI	ATTAAT	1 cut(s)	987
Asp700I	GAANNNNTTC	3 cut(s)	617, 671, 797
AspA2I	CCTAGG	1 cut(s)	43
AspLEI	GCGC	2 cut(s)	1191, 1961
AspS9I	GGNCC	1 cut(s)	2057
AsuHPI	GGTGA	7 cut(s)	218, 430, 622, 1124, 1451, 1502, 1658
AsuII	TTCGAA	2 cut(s)	303, 1482
AsuNHI	GCTAGC	1 cut(s)	1568
AvaII	GGWCC	1 cut(s)	2057
AvrII	CCTAGG	1 cut(s)	43
AxyI	CCTNAGG	1 cut(s)	1761
BauI	CACGAG	1 cut(s)	1312
BbrPI	CACGTG	1 cut(s)	373
Bbv12I	GWGCWC	2 cut(s)	766, 1313
BbvI	GCAGC	1 cut(s)	1537
BccI	CCATC	4 cut(s)	492, 917, 1832, 1892
BceAI	ACGGC	1 cut(s)	1897
BcgI	CGANNNNNNTGC	2 cut(s)	227, 261
BciT130I	CCWGG	3 cut(s)	1212, 1425, 1698
BciVI	GTATCC	2 cut(s)	951, 1166
BcoDI	GTCTC	2 cut(s)	377, 1940
BcuI	ACTAGT	1 cut(s)	1903
BfaI	CTAG	6 cut(s)	44, 143, 512, 1569, 1641, 1904
BfoI	RGCGCY	1 cut(s)	1962
BfuI	GTATCC	2 cut(s)	951, 1166
BglII	AGATCT	1 cut(s)	1259
BisI	GCNGC	2 cut(s)	39, 1551
BlnI	CCTAGG	1 cut(s)	43
BlpI	GCTNAGC	2 cut(s)	1059, 1244
BlsI	GCNGC	2 cut(s)	40, 1552
BmcAI	AGTACT	1 cut(s)	1620
Bme1390I	CCNGG	3 cut(s)	1212, 1425, 1698
Bme18I	GGWCC	1 cut(s)	2057
BmeRI	GACNNNNNGTC	1 cut(s)	404
BmgT120I	GGNCC	1 cut(s)	2057
BmiI	GGNNCC	4 cut(s)	321, 1683, 1849, 2085
BmrFI	CCNGG	3 cut(s)	1212, 1425, 1698
BmsI	GCATC	7 cut(s)	196, 1035, 1211, 1410, 1722, 2157, 2164
BmtI	GCTAGC	1 cut(s)	1572
Bpu1102I	GCTNAGC	2 cut(s)	1059, 1244
Bpu14I	TTCGAA	2 cut(s)	303, 1482
BpuEI	CTTGAG	2 cut(s)	270, 565
BsaAI	YACGTR	1 cut(s)	373
BsaHI	GRCGYC	3 cut(s)	150, 2019, 2071
BsaI	GGTCTC	1 cut(s)	1940
BsaJI	CCNNGG	3 cut(s)	43, 696, 1440
BsaWI	WCCGGW	2 cut(s)	2041, 2100
BsaXI	ACNNNNNCTCC	2 cut(s)	1673, 1703
Bsc4I	CCNNNNNNNGG	1 cut(s)	2093
Bse118I	RCCGGY	3 cut(s)	498, 1850, 2015
Bse21I	CCTNAGG	1 cut(s)	1761
Bse3DI	GCAATG	1 cut(s)	1278
BseBI	CCWGG	3 cut(s)	1212, 1425, 1698
BseDI	CCNNGG	3 cut(s)	43, 696, 1440
BseGI	GGATG	8 cut(s)	199, 435, 1125, 1159, 1513, 2035, 2148, 2179
BseLI	CCNNNNNNNGG	1 cut(s)	2093
BseMI	GCAATG	1 cut(s)	1278
BseMII	CTCAG	7 cut(s)	90, 150, 1254, 1270, 1677, 1752, 1856
BseRI	GAGGAG	1 cut(s)	1694
BseXI	GCAGC	1 cut(s)	1537
Bsh1236I	CGCG	1 cut(s)	1189
Bsh1285I	CGRYCG	2 cut(s)	9, 1878
BshFI	GGCC	2 cut(s)	833, 2005
BsiEI	CGRYCG	2 cut(s)	9, 1878
BsiHKAI	GWGCWC	2 cut(s)	766, 1313
BsiSI	CCGG	5 cut(s)	499, 1851, 2016, 2042, 2101
BslFI	GGGAC	1 cut(s)	538
BslI	CCNNNNNNNGG	1 cut(s)	2093
BsmAI	GTCTC	2 cut(s)	377, 1940
BsmFI	GGGAC	1 cut(s)	538
BsnI	GGCC	2 cut(s)	833, 2005
Bso31I	GGTCTC	1 cut(s)	1940
Bsp119I	TTCGAA	2 cut(s)	303, 1482
Bsp1286I	GDGCHC	2 cut(s)	766, 1313
Bsp1407I	TGTACA	2 cut(s)	809, 1702
Bsp143I	GATC	6 cut(s)	25, 1214, 1259, 1456, 1789, 1914
Bsp1720I	GCTNAGC	2 cut(s)	1059, 1244
BspACI	CCGC	6 cut(s)	39, 1187, 1831, 1869, 1874, 1932
BspANI	GGCC	2 cut(s)	833, 2005
BspCNI	CTCAG	7 cut(s)	91, 151, 1255, 1269, 1678, 1753, 1857
BspFNI	CGCG	1 cut(s)	1189
BspHI	TCATGA	1 cut(s)	529
BspLI	GGNNCC	4 cut(s)	321, 1683, 1849, 2085
BspOI	GCTAGC	1 cut(s)	1572
BspPI	GGATC	4 cut(s)	33, 1222, 1784, 1922
BspQI	GCTCTTC	1 cut(s)	60
BspT104I	TTCGAA	2 cut(s)	303, 1482
BspTNI	GGTCTC	1 cut(s)	1940
BsrBI	CCGCTC	2 cut(s)	1869, 1874
BsrDI	GCAATG	1 cut(s)	1278
BsrFI	RCCGGY	3 cut(s)	498, 1850, 2015
BsrGI	TGTACA	2 cut(s)	809, 1702
BssAI	RCCGGY	3 cut(s)	498, 1850, 2015
BssECI	CCNNGG	3 cut(s)	43, 696, 1440
BssMI	GATC	6 cut(s)	25, 1214, 1259, 1456, 1789, 1914
BssNAI	GTATAC	1 cut(s)	1284
BssNI	GRCGYC	3 cut(s)	150, 2019, 2071
BssSI	CACGAG	1 cut(s)	1312
BssT1I	CCWWGG	2 cut(s)	43, 696
Bst1107I	GTATAC	1 cut(s)	1284
Bst2BI	CACGAG	1 cut(s)	1312
Bst2UI	CCWGG	3 cut(s)	1212, 1425, 1698
Bst4CI	ACNGT	5 cut(s)	10, 733, 1099, 1538, 1562
Bst6I	CTCTTC	1 cut(s)	60
BstACI	GRCGYC	3 cut(s)	150, 2019, 2071
BstAUI	TGTACA	2 cut(s)	809, 1702
BstBAI	YACGTR	1 cut(s)	373
BstBI	TTCGAA	2 cut(s)	303, 1482
BstC8I	GCNNGC	9 cut(s)	448, 500, 1200, 1570, 1657, 1711, 2013, 2017, 2146
BstDSI	CCRYGG	1 cut(s)	1440
BstENI	CCTNNNNNAGG	1 cut(s)	2091
BstF5I	GGATG	8 cut(s)	199, 435, 1125, 1159, 1513, 2035, 2148, 2179
BstFNI	CGCG	1 cut(s)	1189
BstH2I	RGCGCY	1 cut(s)	1962
BstHHI	GCGC	2 cut(s)	1191, 1961
BstKTI	GATC	6 cut(s)	28, 1217, 1262, 1459, 1792, 1917
BstMAI	GTCTC	2 cut(s)	377, 1940
BstMBI	GATC	6 cut(s)	25, 1214, 1259, 1456, 1789, 1914
BstMCI	CGRYCG	2 cut(s)	9, 1878
BstMWI	GCNNNNNNNGC	6 cut(s)	456, 770, 1195, 1383, 1938, 2077
BstNI	CCWGG	3 cut(s)	1212, 1425, 1698
BstNSI	RCATGY	2 cut(s)	1336, 1676
BstSCI	CCNGG	3 cut(s)	1210, 1423, 1696
BstUI	CGCG	1 cut(s)	1189
BstV1I	GCAGC	1 cut(s)	1537
BstX2I	RGATCY	3 cut(s)	1214, 1259, 1914
BstYI	RGATCY	3 cut(s)	1214, 1259, 1914
BstZ17I	GTATAC	1 cut(s)	1284
Bsu36I	CCTNAGG	1 cut(s)	1761
BsuI	GTATCC	2 cut(s)	951, 1166
BsuRI	GGCC	2 cut(s)	833, 2005
BtgI	CCRYGG	1 cut(s)	1440
BtgZI	GCGATG	1 cut(s)	1263
BtsCI	GGATG	8 cut(s)	199, 435, 1125, 1159, 1513, 2035, 2148, 2179
BtsIMutI	CAGTG	2 cut(s)	599, 1095
Cac8I	GCNNGC	9 cut(s)	448, 500, 1200, 1570, 1657, 1711, 2013, 2017, 2146
CaiI	CAGNNNCTG	1 cut(s)	818
CciI	TCATGA	1 cut(s)	529
CfoI	GCGC	2 cut(s)	1191, 1961
Cfr10I	RCCGGY	3 cut(s)	498, 1850, 2015
Cfr13I	GGNCC	1 cut(s)	2057
CseI	GACGC	2 cut(s)	2008, 2060
Csp6I	GTAC	8 cut(s)	256, 810, 1100, 1619, 1703, 1982, 2104, 2168
CspCI	CAANNNNNGTGG	2 cut(s)	1215, 1250
CviQI	GTAC	8 cut(s)	256, 810, 1100, 1619, 1703, 1982, 2104, 2168
DpnI	GATC	6 cut(s)	27, 1216, 1261, 1458, 1791, 1916
DpnII	GATC	6 cut(s)	25, 1214, 1259, 1456, 1789, 1914
DraI	TTTAAA	1 cut(s)	783
DrdI	GACNNNNNNGTC	1 cut(s)	27
DriI	GACNNNNNGTC	1 cut(s)	404
DseDI	GACNNNNNNGTC	1 cut(s)	27
Eam1104I	CTCTTC	1 cut(s)	60
Eam1105I	GACNNNNNGTC	1 cut(s)	404
EarI	CTCTTC	1 cut(s)	60
Eco130I	CCWWGG	2 cut(s)	43, 696
Eco31I	GGTCTC	1 cut(s)	1940
Eco32I	GATATC	1 cut(s)	1093
Eco47I	GGWCC	1 cut(s)	2057
Eco57I	CTGAAG	3 cut(s)	92, 604, 686
Eco72I	CACGTG	1 cut(s)	373
Eco81I	CCTNAGG	1 cut(s)	1761
EcoNI	CCTNNNNNAGG	1 cut(s)	2091
EcoRII	CCWGG	3 cut(s)	1210, 1423, 1696
EcoRV	GATATC	1 cut(s)	1093
EcoT14I	CCWWGG	2 cut(s)	43, 696
ErhI	CCWWGG	2 cut(s)	43, 696
FalI	AAGNNNNNCTT	4 cut(s)	659, 691, 2016, 2048
FaqI	GGGAC	1 cut(s)	538
FauNDI	CATATG	3 cut(s)	562, 1168, 1801
FblI	GTMKAC	2 cut(s)	1283, 1878
Fnu4HI	GCNGC	2 cut(s)	39, 1551
FokI	GGATG	8 cut(s)	206, 442, 1112, 1166, 1520, 2022, 2135, 2186
Fsp4HI	GCNGC	2 cut(s)	39, 1551
FspBI	CTAG	6 cut(s)	44, 143, 512, 1569, 1641, 1904
GlaI	GCGC	2 cut(s)	1190, 1960
GluI	GCNGC	2 cut(s)	39, 1551
HaeII	RGCGCY	1 cut(s)	1962
HaeIII	GGCC	2 cut(s)	833, 2005
HapII	CCGG	5 cut(s)	499, 1851, 2016, 2042, 2101
HgaI	GACGC	2 cut(s)	2008, 2060
HhaI	GCGC	2 cut(s)	1191, 1961
Hin1I	GRCGYC	3 cut(s)	150, 2019, 2071
Hin6I	GCGC	2 cut(s)	1189, 1959
HinP1I	GCGC	2 cut(s)	1189, 1959
HincII	GTYRAC	1 cut(s)	1879
HindII	GTYRAC	1 cut(s)	1879
HinfI	GANTC	7 cut(s)	353, 394, 526, 1588, 1608, 1909, 2097
HpaII	CCGG	5 cut(s)	499, 1851, 2016, 2042, 2101
HphI	GGTGA	7 cut(s)	218, 430, 622, 1124, 1451, 1502, 1658
Hpy166II	GTNNAC	3 cut(s)	1284, 1703, 1879
Hpy8I	GTNNAC	3 cut(s)	1284, 1703, 1879
Hpy99I	CGWCG	9 cut(s)	152, 507, 1344, 1542, 1883, 1958, 2024, 2070, 2076
HpyAV	CCTTC	6 cut(s)	73, 197, 269, 1139, 2038, 2042
HpyCH4III	ACNGT	5 cut(s)	10, 733, 1099, 1538, 1562
HpyCH4IV	ACGT	4 cut(s)	150, 372, 969, 1339
HpyF10VI	GCNNNNNNNGC	6 cut(s)	456, 770, 1195, 1383, 1938, 2077
HpySE526I	ACGT	4 cut(s)	150, 372, 969, 1339
Hsp92I	GRCGYC	3 cut(s)	150, 2019, 2071
HspAI	GCGC	2 cut(s)	1189, 1959
KroI	GCCGGC	2 cut(s)	498, 2015
KroNI	GCCGGC	2 cut(s)	500, 2017
Kzo9I	GATC	6 cut(s)	25, 1214, 1259, 1456, 1789, 1914
LguI	GCTCTTC	1 cut(s)	60
LmnI	GCTCC	6 cut(s)	486, 556, 1681, 1847, 1871, 2083
Lsp1109I	GCAGC	1 cut(s)	1537
LweI	GCATC	7 cut(s)	196, 1035, 1211, 1410, 1722, 2157, 2164
MaeI	CTAG	6 cut(s)	44, 143, 512, 1569, 1641, 1904
MaeII	ACGT	4 cut(s)	150, 372, 969, 1339
MalI	GATC	6 cut(s)	27, 1216, 1261, 1458, 1791, 1916
MbiI	CCGCTC	2 cut(s)	1869, 1874
MboI	GATC	6 cut(s)	25, 1214, 1259, 1456, 1789, 1914
MfeI	CAATTG	2 cut(s)	1049, 1161
MflI	RGATCY	3 cut(s)	1214, 1259, 1914
MhlI	GDGCHC	2 cut(s)	766, 1313
MlyI	GAGTC	3 cut(s)	362, 520, 1903
MmeI	TCCRAC	2 cut(s)	1136, 1485
MreI	CGCCGGCG	1 cut(s)	2015
MroNI	GCCGGC	2 cut(s)	498, 2015
MroXI	GAANNNNTTC	3 cut(s)	617, 671, 797
MseI	TTAA	4 cut(s)	422, 782, 987, 1769
MslI	CAYNNNNRTG	4 cut(s)	192, 854, 1137, 1418
MspI	CCGG	5 cut(s)	499, 1851, 2016, 2042, 2101
MspR9I	CCNGG	3 cut(s)	1212, 1425, 1698
MunI	CAATTG	2 cut(s)	1049, 1161
MvaI	CCWGG	3 cut(s)	1212, 1425, 1698
MvnI	CGCG	1 cut(s)	1189
MwoI	GCNNNNNNNGC	6 cut(s)	456, 770, 1195, 1383, 1938, 2077
NaeI	GCCGGC	2 cut(s)	500, 2017
NdeI	CATATG	3 cut(s)	562, 1168, 1801
NdeII	GATC	6 cut(s)	25, 1214, 1259, 1456, 1789, 1914
NgoMIV	GCCGGC	2 cut(s)	498, 2015
NheI	GCTAGC	1 cut(s)	1568
NlaIV	GGNNCC	4 cut(s)	321, 1683, 1849, 2085
NmeAIII	GCCGAG	1 cut(s)	1917
NmuCI	GTSAC	8 cut(s)	151, 206, 856, 1121, 1445, 1495, 1646, 1906
NspI	RCATGY	2 cut(s)	1336, 1676
NspV	TTCGAA	2 cut(s)	303, 1482
OliI	CACNNNNGTG	2 cut(s)	854, 1137
PagI	TCATGA	1 cut(s)	529
PciI	ACATGT	1 cut(s)	1332
PciSI	GCTCTTC	1 cut(s)	60
PcsI	WCGNNNNNNNCGW	4 cut(s)	2019, 2071, 2193, 2202
PdiI	GCCGGC	2 cut(s)	500, 2017
PdmI	GAANNNNTTC	3 cut(s)	617, 671, 797
PfeI	GAWTC	4 cut(s)	394, 1588, 1608, 2097
PflFI	GACNNNGTC	1 cut(s)	149
PkrI	GCNGC	2 cut(s)	40, 1552
PleI	GAGTC	3 cut(s)	361, 520, 1903
PmaCI	CACGTG	1 cut(s)	373
PmlI	CACGTG	1 cut(s)	373
PpsI	GAGTC	3 cut(s)	361, 520, 1903
Ppu21I	YACGTR	1 cut(s)	373
PscI	ACATGT	1 cut(s)	1332
PshBI	ATTAAT	1 cut(s)	987
PsiI	TTATAA	3 cut(s)	173, 755, 1722
Psp6I	CCWGG	3 cut(s)	1210, 1423, 1696
PspCI	CACGTG	1 cut(s)	373
PspGI	CCWGG	3 cut(s)	1210, 1423, 1696
PspN4I	GGNNCC	4 cut(s)	321, 1683, 1849, 2085
PspPI	GGNCC	1 cut(s)	2057
PstNI	CAGNNNCTG	1 cut(s)	818
PsuI	RGATCY	3 cut(s)	1214, 1259, 1914
PsyI	GACNNNGTC	1 cut(s)	149
RsaI	GTAC	8 cut(s)	257, 811, 1101, 1620, 1704, 1983, 2105, 2169
RsaNI	GTAC	8 cut(s)	256, 810, 1100, 1619, 1703, 1982, 2104, 2168
RseI	CAYNNNNRTG	4 cut(s)	192, 854, 1137, 1418
SalI	GTCGAC	1 cut(s)	1877
SapI	GCTCTTC	1 cut(s)	60
SaqAI	TTAA	4 cut(s)	422, 782, 987, 1769
SatI	GCNGC	2 cut(s)	39, 1551
Sau3AI	GATC	6 cut(s)	25, 1214, 1259, 1456, 1789, 1914
Sau96I	GGNCC	1 cut(s)	2057
ScaI	AGTACT	1 cut(s)	1620
SchI	GAGTC	3 cut(s)	362, 520, 1903
ScrFI	CCNGG	3 cut(s)	1212, 1425, 1698
SduI	GDGCHC	2 cut(s)	766, 1313
SfaNI	GCATC	7 cut(s)	196, 1035, 1211, 1410, 1722, 2157, 2164
SfuI	TTCGAA	2 cut(s)	303, 1482
SgrAI	CRCCGGYG	1 cut(s)	2015
SinI	GGWCC	1 cut(s)	2057
SmiMI	CAYNNNNRTG	4 cut(s)	192, 854, 1137, 1418
SmlI	CTYRAG	2 cut(s)	285, 580
SmoI	CTYRAG	2 cut(s)	285, 580
SpeI	ACTAGT	1 cut(s)	1903
SsiI	CCGC	6 cut(s)	39, 1187, 1831, 1869, 1874, 1932
SspI	AATATT	1 cut(s)	1183
SspMI	CTAG	6 cut(s)	44, 143, 512, 1569, 1641, 1904
StyD4I	CCNGG	3 cut(s)	1210, 1423, 1696
StyI	CCWWGG	2 cut(s)	43, 696
TaaI	ACNGT	5 cut(s)	10, 733, 1099, 1538, 1562
TaiI	ACGT	4 cut(s)	153, 375, 972, 1342
TaqI	TCGA	8 cut(s)	237, 303, 397, 1482, 1878, 2022, 2074, 2196
TatI	WGTACW	4 cut(s)	809, 1099, 1618, 1702
TauI	GCSGC	1 cut(s)	41
TfiI	GAWTC	4 cut(s)	394, 1588, 1608, 2097
Tru1I	TTAA	4 cut(s)	422, 782, 987, 1769
Tru9I	TTAA	4 cut(s)	422, 782, 987, 1769
TscAI	CASTG	2 cut(s)	606, 1102
TseFI	GTSAC	8 cut(s)	151, 206, 856, 1121, 1445, 1495, 1646, 1906
TseI	GCWGC	1 cut(s)	1550
Tsp45I	GTSAC	8 cut(s)	151, 206, 856, 1121, 1445, 1495, 1646, 1906
TspGWI	ACGGA	3 cut(s)	116, 284, 1429
TspRI	CASTG	2 cut(s)	606, 1102
Tth111I	GACNNNGTC	1 cut(s)	149
VpaK11BI	GGWCC	1 cut(s)	2057
VspI	ATTAAT	1 cut(s)	987
XagI	CCTNNNNNAGG	1 cut(s)	2091
XapI	RAATTY	4 cut(s)	641, 1297, 1741, 2192
XceI	RCATGY	2 cut(s)	1336, 1676
XmaJI	CCTAGG	1 cut(s)	43
XmiI	GTMKAC	2 cut(s)	1283, 1878
XmnI	GAANNNNTTC	3 cut(s)	617, 671, 797
XspI	CTAG	6 cut(s)	44, 143, 512, 1569, 1641, 1904
ZraI	GACGTC	1 cut(s)	151
ZrmI	AGTACT	1 cut(s)	1620

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.