MD06G1215200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0013424
Physical Location & Seq
Reverse (-)
34747478 .. 34748869
1392 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1215200.v1.1.491

Sequence Viewer

Length: 597 bp
ATGGCTCTAGCCATAGCCAACAGTGGAAGTTCCACCGGCGGAGCTAGAGTCCCTTACAGCAGCTCCACCACCAGACAGTACCCCTACACCAACCTCTCCTCACAACGCCAGCTCCTCTTCCCTCCCTCCTCCAGAAGAGCCCTTCACGTGGTGGCAGCCAAGAGGTTCACCTCCAGAACTGGAAGGACCGACGGCAAGAACAGGAGGAGCAACACAACCACCAGAGACCAAGAACAAGACATGATGGGGTCGCAACGAACGGCTGAGATTGAAAACGTCGGTGGTGGGGCTGTGGGAAACGTTGATGATGGGTATTTCTTGCCTAAGCTTCCCGGGGATGAACCTTGCTTTTGGGAAGGTGAAAAATGGGACTGGCTTGGATTCTTTGTTGAGTACTTGTGGGCTTTTGGTATTGGCTTTGCGCTTATTGCTTGTTTAGTTGCTGCTACTTCTTACAATGAAGGAGCAACTGATTTCAAGGAAACTCCAGTCTACAAAGATTCGTTCCAGTCTCGGGAGTTTTTGGAAGAACCGGAGGCATCCAACCCGGATGTCTTCGAATCCAACCCGACTGAAGTGGCTCCTAGTTTGGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

199

Amino Acids

21.88

Weight (kDa)

4.88

Isoelectric Point (pI)

52.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013424)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G73885
fragaria_vesca FvH4_5g12290
malus_domestica MD06G1215200.v1.1 MD14G1226000.v1.1
prunus_persica Prupe.5G220500_v2.0.a1
pyrus_communis pycom06g19180 pycom14g18750
rosa_chinensis RchiOBHm_Chr7g0185261
rosa_laevigata RLG00000004926
rosa_multiflora Rmu_co7958283.1_g000001 Rmu_sc0008496.1_g000017
rosa_roxburghii Rroxscaffold_3G00269100
rosa_rugosa Rorug06G0467400
rosa_samantha Rh7AG075600 Rh7BG071900 Rh7CG073100 Rh7DG072500
rosa_wichuraiana Rw7G006030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 492
AciI CCGC 1 cut(s) 39
AclI AACGTT 1 cut(s) 300
AcuI CTGAAG 1 cut(s) 594
AcvI CACGTG 1 cut(s) 148
AdeI CACNNNGTG 1 cut(s) 151
AfaI GTAC 2 cut(s) 80, 395
AfiI CCNNNNNNNGG 2 cut(s) 148, 514
AgsI TTSAA 2 cut(s) 272, 478
AluBI AGCT 4 cut(s) 44, 63, 112, 328
AluI AGCT 4 cut(s) 44, 63, 112, 328
Alw26I GTCTC 2 cut(s) 219, 516
Ama87I CYCGRG 2 cut(s) 332, 513
ApeKI GCWGC 3 cut(s) 60, 155, 443
AspLEI GCGC 1 cut(s) 424
AspS9I GGNCC 1 cut(s) 186
AsuC2I CCSGG 3 cut(s) 333, 334, 548
AsuHPI GGTGA 2 cut(s) 160, 371
AsuII TTCGAA 1 cut(s) 558
AvaI CYCGRG 2 cut(s) 332, 513
AvaII GGWCC 1 cut(s) 186
BanII GRGCYC 1 cut(s) 142
BbrPI CACGTG 1 cut(s) 148
BbsI GAAGAC 1 cut(s) 547
BbvI GCAGC 3 cut(s) 72, 167, 430
BccI CCATC 2 cut(s) 238, 302
BceAI ACGGC 2 cut(s) 208, 276
BcnI CCSGG 3 cut(s) 333, 334, 548
BcoDI GTCTC 2 cut(s) 219, 516
BfaI CTAG 3 cut(s) 8, 45, 585
BisI GCNGC 3 cut(s) 61, 156, 444
BlsI GCNGC 3 cut(s) 62, 157, 445
BmcAI AGTACT 1 cut(s) 395
Bme1390I CCNGG 3 cut(s) 333, 334, 548
Bme18I GGWCC 1 cut(s) 186
BmeT110I CYCGRG 2 cut(s) 332, 513
BmgT120I GGNCC 1 cut(s) 186
BmiI GGNNCC 1 cut(s) 582
BmrFI CCNGG 3 cut(s) 333, 334, 548
BmsI GCATC 1 cut(s) 548
BpiI GAAGAC 1 cut(s) 547
BpmI CTGGAG 3 cut(s) 115, 157, 471
Bpu10I CCTNAGC 1 cut(s) 324
Bpu14I TTCGAA 1 cut(s) 558
BpuMI CCSGG 3 cut(s) 333, 334, 548
BsaAI YACGTR 1 cut(s) 148
BsaI GGTCTC 1 cut(s) 219
BsaJI CCNNGG 2 cut(s) 332, 333
BsaWI WCCGGW 1 cut(s) 532
BsaXI ACNNNNNCTCC 6 cut(s) 33, 47, 63, 77, 96, 126
Bsc4I CCNNNNNNNGG 2 cut(s) 148, 514
Bse118I RCCGGY 1 cut(s) 35
Bse1I ACTGG 4 cut(s) 184, 377, 488, 508
BseDI CCNNGG 2 cut(s) 332, 333
BseGI GGATG 3 cut(s) 343, 539, 556
BseLI CCNNNNNNNGG 2 cut(s) 148, 514
BseMII CTCAG 1 cut(s) 255
BseNI ACTGG 4 cut(s) 184, 377, 488, 508
BseRI GAGGAG 4 cut(s) 88, 104, 118, 220
BseXI GCAGC 3 cut(s) 72, 167, 430
BsiHKCI CYCGRG 2 cut(s) 332, 513
BsiSI CCGG 4 cut(s) 36, 333, 533, 548
BslFI GGGAC 2 cut(s) 35, 383
BslI CCNNNNNNNGG 2 cut(s) 148, 514
BsmAI GTCTC 2 cut(s) 219, 516
BsmFI GGGAC 2 cut(s) 35, 383
Bso31I GGTCTC 1 cut(s) 219
BsoBI CYCGRG 2 cut(s) 332, 513
Bsp119I TTCGAA 1 cut(s) 558
Bsp1286I GDGCHC 1 cut(s) 142
BspACI CCGC 1 cut(s) 39
BspCNI CTCAG 1 cut(s) 256
BspLI GGNNCC 1 cut(s) 582
BspQI GCTCTTC 1 cut(s) 130
BspT104I TTCGAA 1 cut(s) 558
BspTNI GGTCTC 1 cut(s) 219
BsrFI RCCGGY 1 cut(s) 35
BsrI ACTGG 4 cut(s) 184, 377, 488, 508
BssAI RCCGGY 1 cut(s) 35
BssECI CCNNGG 2 cut(s) 332, 333
Bst4CI ACNGT 2 cut(s) 23, 78
Bst6I CTCTTC 2 cut(s) 122, 130
BstBAI YACGTR 1 cut(s) 148
BstBI TTCGAA 1 cut(s) 558
BstC8I GCNNGC 1 cut(s) 110
BstDEI CTNAG 2 cut(s) 264, 324
BstF5I GGATG 3 cut(s) 343, 539, 556
BstHHI GCGC 1 cut(s) 424
BstMAI GTCTC 2 cut(s) 219, 516
BstMWI GCNNNNNNNGC 1 cut(s) 428
BstSCI CCNGG 3 cut(s) 331, 332, 546
BstV1I GCAGC 3 cut(s) 72, 167, 430
BstV2I GAAGAC 1 cut(s) 547
BtsCI GGATG 3 cut(s) 343, 539, 556
BtsIMutI CAGTG 1 cut(s) 28
Cac8I GCNNGC 1 cut(s) 110
CfoI GCGC 1 cut(s) 424
Cfr10I RCCGGY 1 cut(s) 35
Cfr13I GGNCC 1 cut(s) 186
Cfr9I CCCGGG 1 cut(s) 332
Csp6I GTAC 2 cut(s) 79, 394
CviAII CATG 1 cut(s) 241
CviQI GTAC 2 cut(s) 79, 394
DdeI CTNAG 2 cut(s) 264, 324
DraIII CACNNNGTG 1 cut(s) 151
Eam1104I CTCTTC 2 cut(s) 122, 130
EarI CTCTTC 2 cut(s) 122, 130
EciI GGCGGA 1 cut(s) 54
Eco24I GRGCYC 1 cut(s) 142
Eco31I GGTCTC 1 cut(s) 219
Eco47I GGWCC 1 cut(s) 186
Eco57I CTGAAG 1 cut(s) 594
Eco72I CACGTG 1 cut(s) 148
Eco88I CYCGRG 2 cut(s) 332, 513
EcoT38I GRGCYC 1 cut(s) 142
FaeI CATG 1 cut(s) 244
FaiI YATR 2 cut(s) 14, 242
FaqI GGGAC 2 cut(s) 35, 383
FatI CATG 1 cut(s) 240
FblI GTMKAC 1 cut(s) 492
Fnu4HI GCNGC 3 cut(s) 61, 156, 444
FokI GGATG 3 cut(s) 350, 526, 563
FriOI GRGCYC 1 cut(s) 142
Fsp4HI GCNGC 3 cut(s) 61, 156, 444
FspBI CTAG 3 cut(s) 8, 45, 585
GlaI GCGC 1 cut(s) 423
GluI GCNGC 3 cut(s) 61, 156, 444
GsuI CTGGAG 3 cut(s) 115, 157, 471
HapII CCGG 4 cut(s) 36, 333, 533, 548
HhaI GCGC 1 cut(s) 424
Hin1II CATG 1 cut(s) 244
Hin6I GCGC 1 cut(s) 422
HinP1I GCGC 1 cut(s) 422
HindIII AAGCTT 1 cut(s) 326
HinfI GANTC 4 cut(s) 48, 381, 500, 560
HpaII CCGG 4 cut(s) 36, 333, 533, 548
HphI GGTGA 2 cut(s) 160, 371
Hpy166II GTNNAC 2 cut(s) 168, 493
Hpy188III TCNNGA 3 cut(s) 132, 174, 515
Hpy8I GTNNAC 2 cut(s) 168, 493
Hpy99I CGWCG 2 cut(s) 194, 281
HpyAV CCTTC 4 cut(s) 152, 177, 350, 455
HpyCH4III ACNGT 2 cut(s) 23, 78
HpyCH4IV ACGT 3 cut(s) 147, 276, 300
HpyF10VI GCNNNNNNNGC 1 cut(s) 428
HpyF3I CTNAG 2 cut(s) 264, 324
HpySE526I ACGT 3 cut(s) 147, 276, 300
Hsp92II CATG 1 cut(s) 244
HspAI GCGC 1 cut(s) 422
LguI GCTCTTC 1 cut(s) 130
LmnI GCTCC 6 cut(s) 41, 68, 117, 207, 464, 586
Lsp1109I GCAGC 3 cut(s) 72, 167, 430
LweI GCATC 1 cut(s) 548
MaeI CTAG 3 cut(s) 8, 45, 585
MaeII ACGT 3 cut(s) 147, 276, 300
MboII GAAGA 4 cut(s) 109, 147, 539, 547
MhlI GDGCHC 1 cut(s) 142
MlyI GAGTC 1 cut(s) 57
MmeI TCCRAC 2 cut(s) 567, 588
MspI CCGG 4 cut(s) 36, 333, 533, 548
MspR9I CCNGG 3 cut(s) 333, 334, 548
MwoI GCNNNNNNNGC 1 cut(s) 428
NciI CCSGG 3 cut(s) 333, 334, 548
NlaIII CATG 1 cut(s) 244
NlaIV GGNNCC 1 cut(s) 582
NspV TTCGAA 1 cut(s) 558
PciSI GCTCTTC 1 cut(s) 130
PfeI GAWTC 3 cut(s) 381, 500, 560
PkrI GCNGC 3 cut(s) 62, 157, 445
PleI GAGTC 1 cut(s) 56
PmaCI CACGTG 1 cut(s) 148
PmlI CACGTG 1 cut(s) 148
PpsI GAGTC 1 cut(s) 56
Ppu21I YACGTR 1 cut(s) 148
Psp1406I AACGTT 1 cut(s) 300
PspCI CACGTG 1 cut(s) 148
PspN4I GGNNCC 1 cut(s) 582
PspPI GGNCC 1 cut(s) 186
RsaI GTAC 2 cut(s) 80, 395
RsaNI GTAC 2 cut(s) 79, 394
SapI GCTCTTC 1 cut(s) 130
SatI GCNGC 3 cut(s) 61, 156, 444
Sau96I GGNCC 1 cut(s) 186
ScaI AGTACT 1 cut(s) 395
SchI GAGTC 1 cut(s) 57
ScrFI CCNGG 3 cut(s) 333, 334, 548
SduI GDGCHC 1 cut(s) 142
SfaNI GCATC 1 cut(s) 548
SfuI TTCGAA 1 cut(s) 558
SgrAI CRCCGGYG 1 cut(s) 35
SinI GGWCC 1 cut(s) 186
SmaI CCCGGG 1 cut(s) 334
SsiI CCGC 1 cut(s) 39
SspMI CTAG 3 cut(s) 8, 45, 585
StyD4I CCNGG 3 cut(s) 331, 332, 546
TaaI ACNGT 2 cut(s) 23, 78
TaiI ACGT 3 cut(s) 150, 279, 303
TaqI TCGA 1 cut(s) 558
TaqII GACCGA 1 cut(s) 203
TatI WGTACW 1 cut(s) 393
TfiI GAWTC 3 cut(s) 381, 500, 560
TscAI CASTG 1 cut(s) 28
TseI GCWGC 3 cut(s) 60, 155, 443
TspDTI ATGAA 2 cut(s) 354, 474
TspMI CCCGGG 1 cut(s) 332
TspRI CASTG 1 cut(s) 28
VpaK11BI GGWCC 1 cut(s) 186
XmaI CCCGGG 1 cut(s) 332
XmiI GTMKAC 1 cut(s) 492
XspI CTAG 3 cut(s) 8, 45, 585
ZrmI AGTACT 1 cut(s) 395
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.