MD14G1226000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0013424
Physical Location & Seq
Reverse (-)
30663624 .. 30667063
3440 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1226000.v1.1.491

Sequence Viewer

Length: 753 bp
ATGGAAACAATTCGGGTCAAGTTTATGGAAAAACTTCCATTATCATCTCCCCCACTCTCATTATCCCATCCACACATACTGAAGAGATATTTCCACCTTGCTCATTTAGCTTTGTGCTGCTACGAACAAAGAGACTCTCACTCTCCTCCCCTCATGGCTCTTACCATAGCCAACAGTGGAAGTTCCACTGGCGGAGCAAGAGTCCTCTACAGCAGCTCCACCACCAGCAGACAGTACCCCTACACCAACCTCTCCTCACAACGCCACCTCCTCTTCCCTCCCTCCTCCAGAAGAGCCCTCTACGTGGTGTCAGCCAAGAGGTTCGCGTCCAGAACTGGGAGGACCGACGGCAGGAACAGGAAAAGCACCTCAACTACAAGAGACCAAGAACAAGACATGATGGGGTCGCAACGGACGGCTGAGATTGAAAACGTAGGTGGTGGGGTTGTGGGAAACGTTGATGATCGGTATTTCTTGCCTAAGCTTCCGGGGGATGAGCCTTGCTTCTGGGAAGGTGAAAAATGGGATTGGCTTGGATTCTTTGTGGAGTACTTGTGGGCTTTTGGTATTGGCTTTGCGCTTATTGCTTGCTTAGTTGCTGCTACTTCTTACAATGAAGGAGCAACTGATTTCAAGGAGACTCCAGTCTACAAAGATTCGTTTCAGTCTCGGGAGTTTTTGGAAGAACCGGAGGCATCCAACCCGGACGTCTTCGAATCCAACCCGACTGAAGTGGCTCCTAGTTTGGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

251

Amino Acids

28.08

Weight (kDa)

5.91

Isoelectric Point (pI)

50.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013424)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G73885
fragaria_vesca FvH4_5g12290
malus_domestica MD06G1215200.v1.1 MD14G1226000.v1.1
prunus_persica Prupe.5G220500_v2.0.a1
pyrus_communis pycom06g19180 pycom14g18750
rosa_chinensis RchiOBHm_Chr7g0185261
rosa_laevigata RLG00000004926
rosa_multiflora Rmu_co7958283.1_g000001 Rmu_sc0008496.1_g000017
rosa_roxburghii Rroxscaffold_3G00269100
rosa_rugosa Rorug06G0467400
rosa_samantha Rh7AG075600 Rh7BG071900 Rh7CG073100 Rh7DG072500
rosa_wichuraiana Rw7G006030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 711
AccI GTMKAC 1 cut(s) 648
AccII CGCG 1 cut(s) 326
AciI CCGC 1 cut(s) 192
AclI AACGTT 1 cut(s) 456
AcuI CTGAAG 2 cut(s) 101, 750
AcyI GRCGYC 1 cut(s) 708
AfaI GTAC 2 cut(s) 236, 551
AfiI CCNNNNNNNGG 3 cut(s) 304, 336, 351
AgsI TTSAA 2 cut(s) 428, 634
AluBI AGCT 3 cut(s) 110, 216, 484
AluI AGCT 3 cut(s) 110, 216, 484
Alw26I GTCTC 4 cut(s) 126, 375, 632, 672
Ama87I CYCGRG 1 cut(s) 669
ApeKI GCWGC 3 cut(s) 117, 213, 599
Asp700I GAANNNNTTC 2 cut(s) 9, 33
AspLEI GCGC 1 cut(s) 580
AspS9I GGNCC 1 cut(s) 342
AsuC2I CCSGG 2 cut(s) 489, 704
AsuHPI GGTGA 1 cut(s) 527
AsuII TTCGAA 1 cut(s) 714
AvaI CYCGRG 1 cut(s) 669
AvaII GGWCC 1 cut(s) 342
BanII GRGCYC 1 cut(s) 298
BbsI GAAGAC 1 cut(s) 703
BbvI GCAGC 3 cut(s) 104, 225, 586
BccI CCATC 2 cut(s) 75, 394
BceAI ACGGC 2 cut(s) 364, 432
BcnI CCSGG 2 cut(s) 489, 704
BcoDI GTCTC 4 cut(s) 126, 375, 632, 672
BfaI CTAG 1 cut(s) 741
BfmI CTRYAG 1 cut(s) 208
BisI GCNGC 3 cut(s) 118, 214, 600
BlsI GCNGC 3 cut(s) 119, 215, 601
BmcAI AGTACT 1 cut(s) 551
Bme1390I CCNGG 2 cut(s) 489, 704
Bme18I GGWCC 1 cut(s) 342
BmeT110I CYCGRG 1 cut(s) 669
BmgT120I GGNCC 1 cut(s) 342
BmiI GGNNCC 1 cut(s) 738
BmrFI CCNGG 2 cut(s) 489, 704
BmrI ACTGGG 1 cut(s) 345
BmsI GCATC 1 cut(s) 704
BmuI ACTGGG 1 cut(s) 345
BoxI GACNNNNGTC 1 cut(s) 644
BpiI GAAGAC 1 cut(s) 703
BpmI CTGGAG 2 cut(s) 271, 627
Bpu10I CCTNAGC 1 cut(s) 480
Bpu14I TTCGAA 1 cut(s) 714
BpuMI CCSGG 2 cut(s) 489, 704
BsaAI YACGTR 1 cut(s) 304
BsaHI GRCGYC 1 cut(s) 708
BsaI GGTCTC 1 cut(s) 375
BsaJI CCNNGG 1 cut(s) 488
BsaWI WCCGGW 1 cut(s) 688
BsaXI ACNNNNNCTCC 8 cut(s) 186, 200, 216, 230, 252, 282, 539, 569
Bsc4I CCNNNNNNNGG 3 cut(s) 304, 336, 351
Bse1I ACTGG 3 cut(s) 193, 340, 644
BseDI CCNNGG 1 cut(s) 488
BseGI GGATG 3 cut(s) 67, 499, 695
BseLI CCNNNNNNNGG 3 cut(s) 304, 336, 351
BseMII CTCAG 1 cut(s) 411
BseNI ACTGG 3 cut(s) 193, 340, 644
BseRI GAGGAG 4 cut(s) 135, 244, 260, 274
BseXI GCAGC 3 cut(s) 104, 225, 586
Bsh1236I CGCG 1 cut(s) 326
BsiHKCI CYCGRG 1 cut(s) 669
BsiSI CCGG 3 cut(s) 488, 689, 704
BslI CCNNNNNNNGG 3 cut(s) 304, 336, 351
BsmAI GTCTC 4 cut(s) 126, 375, 632, 672
Bso31I GGTCTC 1 cut(s) 375
BsoBI CYCGRG 1 cut(s) 669
Bsp119I TTCGAA 1 cut(s) 714
Bsp1286I GDGCHC 1 cut(s) 298
Bsp143I GATC 1 cut(s) 463
BspACI CCGC 1 cut(s) 192
BspCNI CTCAG 1 cut(s) 412
BspFNI CGCG 1 cut(s) 326
BspLI GGNNCC 1 cut(s) 738
BspQI GCTCTTC 1 cut(s) 286
BspT104I TTCGAA 1 cut(s) 714
BspTNI GGTCTC 1 cut(s) 375
BsrI ACTGG 3 cut(s) 193, 340, 644
BssECI CCNNGG 1 cut(s) 488
BssMI GATC 1 cut(s) 463
BssNI GRCGYC 1 cut(s) 708
Bst4CI ACNGT 2 cut(s) 176, 234
Bst6I CTCTTC 3 cut(s) 77, 278, 286
BstACI GRCGYC 1 cut(s) 708
BstBAI YACGTR 1 cut(s) 304
BstBI TTCGAA 1 cut(s) 714
BstC8I GCNNGC 1 cut(s) 589
BstDEI CTNAG 3 cut(s) 420, 480, 592
BstF5I GGATG 3 cut(s) 67, 499, 695
BstFNI CGCG 1 cut(s) 326
BstHHI GCGC 1 cut(s) 580
BstKTI GATC 1 cut(s) 466
BstMAI GTCTC 4 cut(s) 126, 375, 632, 672
BstMBI GATC 1 cut(s) 463
BstMWI GCNNNNNNNGC 2 cut(s) 107, 584
BstPAI GACNNNNGTC 1 cut(s) 644
BstSCI CCNGG 2 cut(s) 487, 702
BstSFI CTRYAG 1 cut(s) 208
BstUI CGCG 1 cut(s) 326
BstV1I GCAGC 3 cut(s) 104, 225, 586
BstV2I GAAGAC 1 cut(s) 703
BtsCI GGATG 3 cut(s) 67, 499, 695
BtsIMutI CAGTG 2 cut(s) 181, 186
Cac8I GCNNGC 1 cut(s) 589
CfoI GCGC 1 cut(s) 580
Cfr13I GGNCC 1 cut(s) 342
CseI GACGC 1 cut(s) 315
Csp6I GTAC 2 cut(s) 235, 550
CviAII CATG 2 cut(s) 154, 397
CviQI GTAC 2 cut(s) 235, 550
DdeI CTNAG 3 cut(s) 420, 480, 592
DpnI GATC 1 cut(s) 465
DpnII GATC 1 cut(s) 463
Eam1104I CTCTTC 3 cut(s) 77, 278, 286
EarI CTCTTC 3 cut(s) 77, 278, 286
EciI GGCGGA 1 cut(s) 207
Eco24I GRGCYC 1 cut(s) 298
Eco31I GGTCTC 1 cut(s) 375
Eco47I GGWCC 1 cut(s) 342
Eco57I CTGAAG 2 cut(s) 101, 750
Eco88I CYCGRG 1 cut(s) 669
EcoT38I GRGCYC 1 cut(s) 298
FaeI CATG 2 cut(s) 157, 400
FaiI YATR 5 cut(s) 26, 77, 155, 167, 398
FatI CATG 2 cut(s) 153, 396
FblI GTMKAC 1 cut(s) 648
Fnu4HI GCNGC 3 cut(s) 118, 214, 600
FokI GGATG 3 cut(s) 54, 506, 682
FriOI GRGCYC 1 cut(s) 298
Fsp4HI GCNGC 3 cut(s) 118, 214, 600
FspBI CTAG 1 cut(s) 741
GlaI GCGC 1 cut(s) 579
GluI GCNGC 3 cut(s) 118, 214, 600
GsuI CTGGAG 2 cut(s) 271, 627
HapII CCGG 3 cut(s) 488, 689, 704
HgaI GACGC 1 cut(s) 315
HhaI GCGC 1 cut(s) 580
Hin1I GRCGYC 1 cut(s) 708
Hin1II CATG 2 cut(s) 157, 400
Hin6I GCGC 1 cut(s) 578
HinP1I GCGC 1 cut(s) 578
HindIII AAGCTT 1 cut(s) 482
HinfI GANTC 6 cut(s) 134, 201, 537, 640, 656, 716
HpaII CCGG 3 cut(s) 488, 689, 704
HphI GGTGA 1 cut(s) 527
Hpy166II GTNNAC 1 cut(s) 649
Hpy188III TCNNGA 3 cut(s) 288, 330, 671
Hpy8I GTNNAC 1 cut(s) 649
Hpy99I CGWCG 1 cut(s) 350
HpyAV CCTTC 2 cut(s) 506, 611
HpyCH4III ACNGT 2 cut(s) 176, 234
HpyCH4IV ACGT 4 cut(s) 303, 432, 456, 708
HpyF10VI GCNNNNNNNGC 2 cut(s) 107, 584
HpyF3I CTNAG 3 cut(s) 420, 480, 592
HpySE526I ACGT 4 cut(s) 303, 432, 456, 708
Hsp92I GRCGYC 1 cut(s) 708
Hsp92II CATG 2 cut(s) 157, 400
HspAI GCGC 1 cut(s) 578
Kzo9I GATC 1 cut(s) 463
LguI GCTCTTC 1 cut(s) 286
LmnI GCTCC 4 cut(s) 194, 221, 620, 742
Lsp1109I GCAGC 3 cut(s) 104, 225, 586
LweI GCATC 1 cut(s) 704
MaeI CTAG 1 cut(s) 741
MaeII ACGT 4 cut(s) 303, 432, 456, 708
MalI GATC 1 cut(s) 465
MboI GATC 1 cut(s) 463
MboII GAAGA 5 cut(s) 94, 265, 303, 695, 703
MhlI GDGCHC 1 cut(s) 298
MluCI AATT 1 cut(s) 9
MlyI GAGTC 3 cut(s) 128, 210, 634
MmeI TCCRAC 2 cut(s) 723, 744
MroXI GAANNNNTTC 2 cut(s) 9, 33
MspI CCGG 3 cut(s) 488, 689, 704
MspR9I CCNGG 2 cut(s) 489, 704
MvnI CGCG 1 cut(s) 326
MwoI GCNNNNNNNGC 2 cut(s) 107, 584
NciI CCSGG 2 cut(s) 489, 704
NdeII GATC 1 cut(s) 463
NlaIII CATG 2 cut(s) 157, 400
NlaIV GGNNCC 1 cut(s) 738
NspV TTCGAA 1 cut(s) 714
PciSI GCTCTTC 1 cut(s) 286
PdmI GAANNNNTTC 2 cut(s) 9, 33
PfeI GAWTC 3 cut(s) 537, 656, 716
PkrI GCNGC 3 cut(s) 119, 215, 601
PleI GAGTC 3 cut(s) 128, 209, 634
PpsI GAGTC 3 cut(s) 128, 209, 634
Ppu21I YACGTR 1 cut(s) 304
PshAI GACNNNNGTC 1 cut(s) 644
Psp1406I AACGTT 1 cut(s) 456
PspN4I GGNNCC 1 cut(s) 738
PspPI GGNCC 1 cut(s) 342
RsaI GTAC 2 cut(s) 236, 551
RsaNI GTAC 2 cut(s) 235, 550
SapI GCTCTTC 1 cut(s) 286
SatI GCNGC 3 cut(s) 118, 214, 600
Sau3AI GATC 1 cut(s) 463
Sau96I GGNCC 1 cut(s) 342
ScaI AGTACT 1 cut(s) 551
SchI GAGTC 3 cut(s) 128, 210, 634
ScrFI CCNGG 2 cut(s) 489, 704
SduI GDGCHC 1 cut(s) 298
SfaNI GCATC 1 cut(s) 704
SfcI CTRYAG 1 cut(s) 208
SfuI TTCGAA 1 cut(s) 714
SinI GGWCC 1 cut(s) 342
Sse9I AATT 1 cut(s) 9
SsiI CCGC 1 cut(s) 192
SspMI CTAG 1 cut(s) 741
StyD4I CCNGG 2 cut(s) 487, 702
TaaI ACNGT 2 cut(s) 176, 234
TaiI ACGT 4 cut(s) 306, 435, 459, 711
TaqI TCGA 1 cut(s) 714
TaqII GACCGA 1 cut(s) 359
TasI AATT 1 cut(s) 9
TatI WGTACW 1 cut(s) 549
TfiI GAWTC 3 cut(s) 537, 656, 716
TscAI CASTG 2 cut(s) 181, 193
TseI GCWGC 3 cut(s) 117, 213, 599
TspDTI ATGAA 1 cut(s) 630
TspGWI ACGGA 1 cut(s) 427
TspRI CASTG 2 cut(s) 181, 193
VpaK11BI GGWCC 1 cut(s) 342
XmiI GTMKAC 1 cut(s) 648
XmnI GAANNNNTTC 2 cut(s) 9, 33
XspI CTAG 1 cut(s) 741
ZraI GACGTC 1 cut(s) 709
ZrmI AGTACT 1 cut(s) 551
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.