Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0007165

Physical Location & Seq

Genomic Coordinates Forward (+)

34975347 .. 34980613

Gene Structure 5267 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1217700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1590 bp

ATGAGGTCGTCGTCTTCCTCCGATAGGATTAACATTGCCGCGAGTGCCCAGCGACTCGATGTCGACAATCGGATTCCTCTGCGTATCTACTTTCGGATCGCCAATAATATCCTCAAGCAGGCTGACATTTTTCGACAAGAGAAGAATATTATAGACCTATATATTATGCTACTAAGGTTTTCAAGTTTGGTCTCTGAGACAATACCGCATCATCGAGACTATAGAGCATCTATTCAAGGAGAGAAGGTTTTCTTGAAAACGAAATTGTTTGATGCTCTGAATGAGCTCGAGGAGCTCAAGCCAGCAGTGAAACAGAAGCTTAAGCCAGCCATGAAACATAAGAACGATGAATTTAACAGAATAAATGGAAAATTTAACGACGGGTGGAGCCATAATAACCAGATTGATTTTGTGGTTTCTCCTCTCAAGAAGCAATCATTGATAAGCTATGATGCAACCAAGGCAGCAAGACCAGCTGCAAGAGAACTTGTCTATCAACCTCCGAGGTCTCAACAATTTTCCTATGCCAGGCCTTTGGAGGGTCAATTTCGCAAACTATCCATAGTTCCACGTCCAAATGAGGAAACTCTTTCCAGACATTCTATTTTGGGCCCAAATGGCCTTAATGGACAGTGGCAGCCACCTAGAAGTGATATAGGGGTTCGCTATCCAAGTATATTAGACTTAACTCCTGTTGAAATTCCAAGGGAAAACAGTGTGGGACAGTCCATAGAAGATGAACATACAATTAAAAAAGACGAAAAGACAGATATAAAGGATGCTAGCGACTTGGAACCTGAAAGGTCAAGTTTGGAACCAATTCTTACCCAGACTACTGATGGTGGCCAGAAGGATTTTACAGAGGAACTTTGCTCCCTTATTTCTTTCGAATCAACAAAAACTCCTGGTCATACAAAACTTATCAGACAGCCTTCTCCTCCTCCTGTTCTTGCGGAAGTACAAGACTTGATTCCAGCAGTTTCACCTCAAGTGTCTGAGGTAGAATGTGGACTAGAGACTCTCTCATCGGATGAACTTCTTCGTGCTGAATCTCCCCTGCAATTACACATTTCAACGACAATGATGGAGCATTTCATGAAGCTGGCTAAGTCAAATACTGATAAGAGCTTGGAGACGTGTGGCATCCTAGGAGGCTCACTAAAAAACAGAAAGTTTTATGTTACAGCTCTCATCATACCTAAGCAGGAGTCAACTGCGAATTCGTGTCAGGCCACGAATGAAGAGGAAATATTTGAAGTTCAGGATAAACAATCTCTCTTTCCTCTTGGCTGGATACATACGCATCCTACGCAGTCTTGTTTCATGTCGTCGATTGATCTCCATACCCATTATTCATATCAGATTATGTTGCCGGAAGCTGTTGCAATTGTCATGGCACCACAAGGTGGCTCAAGAACCCATGGCATTTTCCGATTGACAAACCCAGGTGGTATGTCGGTCATTAGACAGTGCCAGCACCGTGGTTTTCATCCACATGATCAACCACCTGATGGTGGACCCATTTACAAGACCTGTGCAGATGTTTATATGAACCCCAATCTAAAGTTTGACGTTATTGATCTTCGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0006464 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016579 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0070536 GO:0070646 GO:0070647 GO:0071108 GO:0071704 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04144 map04144

Pfam Domains

Protein Families

JAB USP8_dimer

Protein Analysis

530

Amino Acids

59.73

Weight (kDa)

6.51

Isoelectric Point (pI)

53.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
USP8_dimer	PF08969	12 - 106	1.4e-12	USP8 dimerisation domain
JAB	PF01398	351 - 459	6.4e-18	JAB1/Mov34/MPN/PAD-1 ubiquitin protease
Prok-JAB	PF14464	369 - 451	3.6e-07	Prokaryotic homologs of the JAB domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007165)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G48790
fragaria_vesca	FvH4_5g11880 FvH4_5g11880 FvH4_5g11880 FvH4_5g11880 FvH4_5g11880 FvH4_5g11880
malus_domestica	MD06G1217700.v1.1 MD14G1227900.v1.1
prunus_persica	Prupe.5G222900_v2.0.a1
pyrus_communis	pycom06g19390 pycom14g18930
rosa_chinensis	RchiOBHm_Chr7g0185701
rosa_laevigata	RLG00000004893
rosa_multiflora	Rmu_sc0004909.1_g000002 Rmu_sc0008747.1_g000004 Rmu_sc0023648.1_g000001
rosa_roxburghii	Rroxscaffold_3G00268720
rosa_rugosa	Rorug06G0470600 Rorug06G0470700
rosa_samantha	Rh7AG071800 Rh7BG076100 Rh7CG076100 Rh7DG076100
rosa_wichuraiana	Rw7G006340

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	1396
AccB7I	CCANNNNNTGG	2 cut(s)	1406, 1511
AccI	GTMKAC	1 cut(s)	63
AccII	CGCG	1 cut(s)	41
AciI	CCGC	3 cut(s)	39, 206, 953
AclWI	GGATC	1 cut(s)	104
AcoI	YGGCCR	1 cut(s)	844
AcsI	RAATTY	4 cut(s)	350, 371, 699, 1219
AdeI	CACNNNGTG	1 cut(s)	1406
AfaI	GTAC	1 cut(s)	960
AfiI	CCNNNNNNNGG	7 cut(s)	24, 118, 528, 539, 1406, 1511, 1514
AflII	CTTAAG	1 cut(s)	320
AflIII	ACRYGT	1 cut(s)	1136
AgsI	TTSAA	6 cut(s)	183, 236, 256, 698, 1074, 1256
AhdI	GACNNNNNGTC	1 cut(s)	59
AjiI	CACGTC	2 cut(s)	572, 1137
AjnI	CCWGG	3 cut(s)	527, 904, 1444
AluBI	AGCT	9 cut(s)	286, 295, 319, 447, 476, 1102, 1128, 1187, 1379
AluI	AGCT	9 cut(s)	286, 295, 319, 447, 476, 1102, 1128, 1187, 1379
Alw21I	GWGCWC	2 cut(s)	288, 297
Alw26I	GTCTC	6 cut(s)	191, 196, 210, 513, 1010, 1127
AlwI	GGATC	1 cut(s)	104
Ama87I	CYCGRG	1 cut(s)	287
AoxI	GGCC	5 cut(s)	530, 610, 619, 844, 1230
ApaI	GGGCCC	1 cut(s)	614
ApeKI	GCWGC	3 cut(s)	464, 476, 637
ApoI	RAATTY	4 cut(s)	350, 371, 699, 1219
Asp700I	GAANNNNTTC	3 cut(s)	248, 819, 1038
AspA2I	CCTAGG	1 cut(s)	1147
AspS9I	GGNCC	3 cut(s)	610, 611, 1517
AsuHPI	GGTGA	1 cut(s)	975
AsuII	TTCGAA	1 cut(s)	888
AsuNHI	GCTAGC	1 cut(s)	782
AvaI	CYCGRG	1 cut(s)	287
AvaII	GGWCC	1 cut(s)	1517
AvrII	CCTAGG	1 cut(s)	1147
BaeGI	GKGCMC	2 cut(s)	49, 614
BalI	TGGCCA	1 cut(s)	846
BanI	GGYRCC	1 cut(s)	1396
BanII	GRGCYC	3 cut(s)	288, 297, 614
BbsI	GAAGAC	1 cut(s)	6
Bbv12I	GWGCWC	2 cut(s)	288, 297
BbvI	GCAGC	3 cut(s)	463, 476, 649
BccI	CCATC	3 cut(s)	833, 1078, 1505
BciT130I	CCWGG	3 cut(s)	529, 906, 1446
BciVI	GTATCC	1 cut(s)	1287
BclI	TGATCA	1 cut(s)	1498
BcoDI	GTCTC	6 cut(s)	191, 196, 210, 513, 1010, 1127
BfaI	CTAG	4 cut(s)	645, 783, 1013, 1148
BfmI	CTRYAG	1 cut(s)	220
BfrI	CTTAAG	1 cut(s)	320
BfuI	GTATCC	1 cut(s)	1287
BglI	GCCNNNNNGGC	1 cut(s)	618
BisI	GCNGC	4 cut(s)	39, 465, 477, 638
BlnI	CCTAGG	1 cut(s)	1147
BlsI	GCNGC	4 cut(s)	40, 466, 478, 639
Bme1390I	CCNGG	3 cut(s)	529, 906, 1446
Bme18I	GGWCC	1 cut(s)	1517
BmeRI	GACNNNNNGTC	1 cut(s)	59
BmeT110I	CYCGRG	1 cut(s)	287
BmgBI	CACGTC	2 cut(s)	572, 1137
BmgT120I	GGNCC	3 cut(s)	610, 611, 1517
BmiI	GGNNCC	6 cut(s)	389, 612, 795, 816, 1398, 1519
BmrFI	CCNGG	3 cut(s)	529, 906, 1446
BmsI	GCATC	7 cut(s)	217, 236, 262, 442, 769, 1152, 1312
BmtI	GCTAGC	1 cut(s)	786
BpiI	GAAGAC	1 cut(s)	6
BplI	GAGNNNNNCTC	2 cut(s)	1007, 1039
Bpu10I	CCTNAGC	1 cut(s)	1200
Bpu14I	TTCGAA	1 cut(s)	888
BpuEI	CTTGAG	5 cut(s)	98, 281, 410, 972, 1396
BsaI	GGTCTC	2 cut(s)	196, 513
BsaJI	CCNNGG	7 cut(s)	459, 503, 704, 1147, 1420, 1444, 1480
BsaXI	ACNNNNNCTCC	4 cut(s)	231, 261, 886, 916
Bsc4I	CCNNNNNNNGG	7 cut(s)	24, 118, 528, 539, 1406, 1511, 1514
Bse3DI	GCAATG	1 cut(s)	33
BseBI	CCWGG	3 cut(s)	529, 906, 1446
BseDI	CCNNGG	7 cut(s)	459, 503, 704, 1147, 1420, 1444, 1480
BseGI	GGATG	5 cut(s)	784, 1036, 1143, 1303, 1489
BseLI	CCNNNNNNNGG	7 cut(s)	24, 118, 528, 539, 1406, 1511, 1514
BseMI	GCAATG	1 cut(s)	33
BseMII	CTCAG	2 cut(s)	186, 987
BseRI	GAGGAG	4 cut(s)	305, 411, 927, 930
BseSI	GKGCMC	2 cut(s)	49, 614
BseXI	GCAGC	3 cut(s)	463, 476, 649
BseYI	CCCAGC	1 cut(s)	48
BsgI	GTGCAG	1 cut(s)	1557
Bsh1236I	CGCG	1 cut(s)	41
BshFI	GGCC	5 cut(s)	532, 612, 621, 846, 1232
BshNI	GGYRCC	1 cut(s)	1396
BsiHKAI	GWGCWC	2 cut(s)	288, 297
BsiHKCI	CYCGRG	1 cut(s)	287
BsiSI	CCGG	1 cut(s)	1373
BslFI	GGGAC	1 cut(s)	735
BslI	CCNNNNNNNGG	7 cut(s)	24, 118, 528, 539, 1406, 1511, 1514
BsmAI	GTCTC	6 cut(s)	191, 196, 210, 513, 1010, 1127
BsmBI	CGTCTC	1 cut(s)	1127
BsmFI	GGGAC	1 cut(s)	735
BsnI	GGCC	5 cut(s)	532, 612, 621, 846, 1232
Bso31I	GGTCTC	2 cut(s)	196, 513
BsoBI	CYCGRG	1 cut(s)	287
Bsp119I	TTCGAA	1 cut(s)	888
Bsp120I	GGGCCC	1 cut(s)	610
Bsp1286I	GDGCHC	4 cut(s)	49, 288, 297, 614
Bsp143I	GATC	4 cut(s)	96, 1336, 1498, 1579
Bsp19I	CCATGG	1 cut(s)	1420
BspACI	CCGC	3 cut(s)	39, 206, 953
BspANI	GGCC	5 cut(s)	532, 612, 621, 846, 1232
BspCNI	CTCAG	2 cut(s)	187, 988
BspFNI	CGCG	1 cut(s)	41
BspHI	TCATGA	1 cut(s)	1095
BspLI	GGNNCC	6 cut(s)	389, 612, 795, 816, 1398, 1519
BspOI	GCTAGC	1 cut(s)	786
BspPI	GGATC	1 cut(s)	104
BspT104I	TTCGAA	1 cut(s)	888
BspT107I	GGYRCC	1 cut(s)	1396
BspTI	CTTAAG	1 cut(s)	320
BspTNI	GGTCTC	2 cut(s)	196, 513
BsrDI	GCAATG	1 cut(s)	33
BssECI	CCNNGG	7 cut(s)	459, 503, 704, 1147, 1420, 1444, 1480
BssMI	GATC	4 cut(s)	96, 1336, 1498, 1579
BssT1I	CCWWGG	4 cut(s)	459, 704, 1147, 1420
Bst2UI	CCWGG	3 cut(s)	529, 906, 1446
Bst4CI	ACNGT	5 cut(s)	633, 716, 726, 1470, 1481
Bst6I	CTCTTC	1 cut(s)	1236
BstAFI	CTTAAG	1 cut(s)	320
BstBI	TTCGAA	1 cut(s)	888
BstC8I	GCNNGC	6 cut(s)	120, 303, 327, 784, 1104, 1475
BstDEI	CTNAG	5 cut(s)	173, 195, 996, 1107, 1200
BstDSI	CCRYGG	2 cut(s)	1420, 1480
BstENI	CCTNNNNNAGG	3 cut(s)	22, 116, 526
BstF5I	GGATG	5 cut(s)	784, 1036, 1143, 1303, 1489
BstFNI	CGCG	1 cut(s)	41
BstKTI	GATC	4 cut(s)	99, 1339, 1501, 1582
BstMAI	GTCTC	6 cut(s)	191, 196, 210, 513, 1010, 1127
BstMBI	GATC	4 cut(s)	96, 1336, 1498, 1579
BstMWI	GCNNNNNNNGC	6 cut(s)	44, 292, 461, 473, 618, 1309
BstNI	CCWGG	3 cut(s)	529, 906, 1446
BstSCI	CCNGG	3 cut(s)	527, 904, 1444
BstSFI	CTRYAG	1 cut(s)	220
BstSLI	GKGCMC	2 cut(s)	49, 614
BstUI	CGCG	1 cut(s)	41
BstV1I	GCAGC	3 cut(s)	463, 476, 649
BstV2I	GAAGAC	1 cut(s)	6
BstXI	CCANNNNNNTGG	2 cut(s)	535, 1481
BsuI	GTATCC	1 cut(s)	1287
BsuRI	GGCC	5 cut(s)	532, 612, 621, 846, 1232
BtgI	CCRYGG	2 cut(s)	1420, 1480
BtrI	CACGTC	2 cut(s)	572, 1137
BtsCI	GGATG	5 cut(s)	784, 1036, 1143, 1303, 1489
BtsI	GCAGTG	1 cut(s)	312
BtsIMutI	CAGTG	4 cut(s)	312, 638, 721, 1475
Cac8I	GCNNGC	6 cut(s)	120, 303, 327, 784, 1104, 1475
CciI	TCATGA	1 cut(s)	1095
Cfr13I	GGNCC	3 cut(s)	610, 611, 1517
Csp6I	GTAC	1 cut(s)	959
CviAII	CATG	6 cut(s)	331, 1096, 1324, 1393, 1421, 1496
CviQI	GTAC	1 cut(s)	959
DdeI	CTNAG	5 cut(s)	173, 195, 996, 1107, 1200
DpnI	GATC	4 cut(s)	98, 1338, 1500, 1581
DpnII	GATC	4 cut(s)	96, 1336, 1498, 1579
DraIII	CACNNNGTG	1 cut(s)	1406
DriI	GACNNNNNGTC	1 cut(s)	59
EaeI	YGGCCR	1 cut(s)	844
Eam1104I	CTCTTC	1 cut(s)	1236
Eam1105I	GACNNNNNGTC	1 cut(s)	59
EarI	CTCTTC	1 cut(s)	1236
Ecl136II	GAGCTC	2 cut(s)	286, 295
Eco130I	CCWWGG	4 cut(s)	459, 704, 1147, 1420
Eco147I	AGGCCT	1 cut(s)	532
Eco24I	GRGCYC	3 cut(s)	288, 297, 614
Eco31I	GGTCTC	2 cut(s)	196, 513
Eco47I	GGWCC	1 cut(s)	1517
Eco53kI	GAGCTC	2 cut(s)	286, 295
Eco88I	CYCGRG	1 cut(s)	287
EcoICRI	GAGCTC	2 cut(s)	286, 295
EcoNI	CCTNNNNNAGG	3 cut(s)	22, 116, 526
EcoRI	GAATTC	1 cut(s)	1219
EcoRII	CCWGG	3 cut(s)	527, 904, 1444
EcoT14I	CCWWGG	4 cut(s)	459, 704, 1147, 1420
EcoT38I	GRGCYC	3 cut(s)	288, 297, 614
ErhI	CCWWGG	4 cut(s)	459, 704, 1147, 1420
Esp3I	CGTCTC	1 cut(s)	1127
FaeI	CATG	6 cut(s)	334, 1099, 1327, 1396, 1424, 1499
FalI	AAGNNNNNCTT	2 cut(s)	236, 268
FaqI	GGGAC	1 cut(s)	735
FatI	CATG	6 cut(s)	330, 1095, 1323, 1392, 1420, 1495
FbaI	TGATCA	1 cut(s)	1498
FblI	GTMKAC	1 cut(s)	63
Fnu4HI	GCNGC	4 cut(s)	39, 465, 477, 638
FokI	GGATG	5 cut(s)	791, 1043, 1130, 1290, 1476
FriOI	GRGCYC	3 cut(s)	288, 297, 614
Fsp4HI	GCNGC	4 cut(s)	39, 465, 477, 638
FspBI	CTAG	4 cut(s)	645, 783, 1013, 1148
GluI	GCNGC	4 cut(s)	39, 465, 477, 638
GsaI	CCCAGC	1 cut(s)	52
HaeIII	GGCC	5 cut(s)	532, 612, 621, 846, 1232
HapII	CCGG	1 cut(s)	1373
Hin1II	CATG	6 cut(s)	334, 1099, 1327, 1396, 1424, 1499
HincII	GTYRAC	2 cut(s)	64, 1212
HindII	GTYRAC	2 cut(s)	64, 1212
HindIII	AAGCTT	1 cut(s)	317
HinfI	GANTC	7 cut(s)	54, 73, 890, 970, 1018, 1049, 1208
HpaII	CCGG	1 cut(s)	1373
HphI	GGTGA	1 cut(s)	975
Hpy166II	GTNNAC	4 cut(s)	64, 1010, 1212, 1517
Hpy188III	TCNNGA	7 cut(s)	215, 253, 427, 594, 1096, 1262, 1413
Hpy8I	GTNNAC	4 cut(s)	64, 1010, 1212, 1517
Hpy99I	CGWCG	3 cut(s)	13, 383, 1333
HpyAV	CCTTC	3 cut(s)	238, 844, 942
HpyCH4III	ACNGT	5 cut(s)	633, 716, 726, 1470, 1481
HpyCH4IV	ACGT	3 cut(s)	571, 1136, 1572
HpyCH4V	TGCA	5 cut(s)	455, 479, 1060, 1385, 1538
HpyF10VI	GCNNNNNNNGC	6 cut(s)	44, 292, 461, 473, 618, 1309
HpyF3I	CTNAG	5 cut(s)	173, 195, 996, 1107, 1200
HpySE526I	ACGT	3 cut(s)	571, 1136, 1572
Hsp92II	CATG	6 cut(s)	334, 1099, 1327, 1396, 1424, 1499
Ksp22I	TGATCA	1 cut(s)	1498
Kzo9I	GATC	4 cut(s)	96, 1336, 1498, 1579
LmnI	GCTCC	4 cut(s)	292, 387, 878, 1087
Lsp1109I	GCAGC	3 cut(s)	463, 476, 649
LweI	GCATC	7 cut(s)	217, 236, 262, 442, 769, 1152, 1312
MaeI	CTAG	4 cut(s)	645, 783, 1013, 1148
MaeII	ACGT	3 cut(s)	571, 1136, 1572
MaeIII	GTNAC	1 cut(s)	1180
MalI	GATC	4 cut(s)	98, 1338, 1500, 1581
MboI	GATC	4 cut(s)	96, 1336, 1498, 1579
MboII	GAAGA	6 cut(s)	6, 154, 746, 1031, 1253, 1574
MfeI	CAATTG	1 cut(s)	1386
MhlI	GDGCHC	4 cut(s)	49, 288, 297, 614
MlsI	TGGCCA	1 cut(s)	846
MluNI	TGGCCA	1 cut(s)	846
MlyI	GAGTC	3 cut(s)	48, 1012, 1217
Mox20I	TGGCCA	1 cut(s)	846
MroXI	GAANNNNTTC	3 cut(s)	248, 819, 1038
MscI	TGGCCA	1 cut(s)	846
MseI	TTAA	7 cut(s)	30, 321, 354, 375, 624, 686, 750
MslI	CAYNNNNRTG	1 cut(s)	1494
Msp20I	TGGCCA	1 cut(s)	846
MspA1I	CMGCKG	1 cut(s)	476
MspCI	CTTAAG	1 cut(s)	320
MspI	CCGG	1 cut(s)	1373
MspR9I	CCNGG	3 cut(s)	529, 906, 1446
MunI	CAATTG	1 cut(s)	1386
MvaI	CCWGG	3 cut(s)	529, 906, 1446
MvnI	CGCG	1 cut(s)	41
MwoI	GCNNNNNNNGC	6 cut(s)	44, 292, 461, 473, 618, 1309
NcoI	CCATGG	1 cut(s)	1420
NdeII	GATC	4 cut(s)	96, 1336, 1498, 1579
NheI	GCTAGC	1 cut(s)	782
NlaIII	CATG	6 cut(s)	334, 1099, 1327, 1396, 1424, 1499
NlaIV	GGNNCC	6 cut(s)	389, 612, 795, 816, 1398, 1519
NspV	TTCGAA	1 cut(s)	888
PaeR7I	CTCGAG	1 cut(s)	287
PagI	TCATGA	1 cut(s)	1095
PceI	AGGCCT	1 cut(s)	532
PdmI	GAANNNNTTC	3 cut(s)	248, 819, 1038
PfeI	GAWTC	4 cut(s)	73, 890, 970, 1049
PflMI	CCANNNNNTGG	2 cut(s)	1406, 1511
PkrI	GCNGC	4 cut(s)	40, 466, 478, 639
PleI	GAGTC	3 cut(s)	48, 1012, 1216
PpsI	GAGTC	3 cut(s)	48, 1012, 1216
Psp124BI	GAGCTC	2 cut(s)	288, 297
Psp6I	CCWGG	3 cut(s)	527, 904, 1444
PspFI	CCCAGC	1 cut(s)	48
PspGI	CCWGG	3 cut(s)	527, 904, 1444
PspN4I	GGNNCC	6 cut(s)	389, 612, 795, 816, 1398, 1519
PspOMI	GGGCCC	1 cut(s)	610
PspPI	GGNCC	3 cut(s)	610, 611, 1517
PspXI	VCTCGAGB	1 cut(s)	287
PvuII	CAGCTG	1 cut(s)	476
RsaI	GTAC	1 cut(s)	960
RsaNI	GTAC	1 cut(s)	959
RseI	CAYNNNNRTG	1 cut(s)	1494
SacI	GAGCTC	2 cut(s)	288, 297
SalI	GTCGAC	1 cut(s)	62
SaqAI	TTAA	7 cut(s)	30, 321, 354, 375, 624, 686, 750
SatI	GCNGC	4 cut(s)	39, 465, 477, 638
Sau3AI	GATC	4 cut(s)	96, 1336, 1498, 1579
Sau96I	GGNCC	3 cut(s)	610, 611, 1517
SchI	GAGTC	3 cut(s)	48, 1012, 1217
ScrFI	CCNGG	3 cut(s)	529, 906, 1446
SduI	GDGCHC	4 cut(s)	49, 288, 297, 614
SfaNI	GCATC	7 cut(s)	217, 236, 262, 442, 769, 1152, 1312
SfcI	CTRYAG	1 cut(s)	220
SfiI	GGCCNNNNNGGCC	1 cut(s)	618
Sfr274I	CTCGAG	1 cut(s)	287
SfuI	TTCGAA	1 cut(s)	888
SinI	GGWCC	1 cut(s)	1517
SlaI	CTCGAG	1 cut(s)	287
SmiMI	CAYNNNNRTG	1 cut(s)	1494
SmlI	CTYRAG	7 cut(s)	113, 287, 296, 320, 425, 987, 1411
SmoI	CTYRAG	7 cut(s)	113, 287, 296, 320, 425, 987, 1411
SseBI	AGGCCT	1 cut(s)	532
SsiI	CCGC	3 cut(s)	39, 206, 953
SspI	AATATT	2 cut(s)	148, 1251
SspMI	CTAG	4 cut(s)	645, 783, 1013, 1148
SstI	GAGCTC	2 cut(s)	288, 297
StuI	AGGCCT	1 cut(s)	532
StyD4I	CCNGG	3 cut(s)	527, 904, 1444
StyI	CCWWGG	4 cut(s)	459, 704, 1147, 1420
TaaI	ACNGT	5 cut(s)	633, 716, 726, 1470, 1481
TaiI	ACGT	3 cut(s)	574, 1139, 1575
TaqI	TCGA	8 cut(s)	57, 63, 133, 214, 288, 888, 1331, 1585
TaqII	GACCGA	1 cut(s)	1447
TatI	WGTACW	1 cut(s)	958
TauI	GCSGC	1 cut(s)	41
TfiI	GAWTC	4 cut(s)	73, 890, 970, 1049
Tru1I	TTAA	7 cut(s)	30, 321, 354, 375, 624, 686, 750
Tru9I	TTAA	7 cut(s)	30, 321, 354, 375, 624, 686, 750
TscAI	CASTG	4 cut(s)	312, 638, 721, 1475
TseI	GCWGC	3 cut(s)	464, 476, 637
TspRI	CASTG	4 cut(s)	312, 638, 721, 1475
Van91I	CCANNNNNTGG	2 cut(s)	1406, 1511
Vha464I	CTTAAG	1 cut(s)	320
VpaK11BI	GGWCC	1 cut(s)	1517
XagI	CCTNNNNNAGG	3 cut(s)	22, 116, 526
XapI	RAATTY	4 cut(s)	350, 371, 699, 1219
XcmI	CCANNNNNNNNNTGG	1 cut(s)	836
XhoI	CTCGAG	1 cut(s)	287
XmaJI	CCTAGG	1 cut(s)	1147
XmiI	GTMKAC	1 cut(s)	63
XmnI	GAANNNNTTC	3 cut(s)	248, 819, 1038
XspI	CTAG	4 cut(s)	645, 783, 1013, 1148

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.