MD06G1225900.v1.1

Protein of unknown function (DUF1118)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0013432
Physical Location & Seq
Forward (+)
35644034 .. 35644994
961 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1225900.v1.1.491

Sequence Viewer

Length: 537 bp
ATGGAGGCCGGAGCATTTTGCAGCAGCGCAGGAGGCGTGCATTTCACTTTCAGAGCGGCGGCGGCGGCAAAAGGGGGTAACTTTGCTTCTTCCCCTCCCCTCCTCATCCAATCCATGGCTACCCAGAAACCCATGCCTTCAGCTACCAAAACACTTAGCTCCAAGAAGAATTCATCTGTGAAGCTACTAACAAGGGTGGAGCAACTGAAGCTACTGAGCAAAGCGGAGAAAGCCGGCCTGCTATCCACGGCAGAGAAGTCCGGGCTCTCATTGTCGACGATAGAGAAGCTAGGGTTGCTCTCCAAAGCGGAGGAATTCGGAATCCTGTCAGCGGCCACCGACCCTGGAACTCCCGGGGCATTGCTGAGCTTAAGCTTAGGACTGCTGGTTTTGGGTCCATTTGTTGTTTATCTTGTGCCTGAGGATTCTCCCTTGGAGATAGGGTTACAAGCTGTTGTTGCTTTGGCTGCAGTAGCTGGTGGTTCAGCTGCTTTTGCTGCATCAAATCTTGTATCCAGCTTGCAGAAATCAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1118

Protein Analysis

179

Amino Acids

17.93

Weight (kDa)

9.3

Isoelectric Point (pI)

39.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1118 PF06549 65 - 177 4.7e-46 Protein of unknown function (DUF1118)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013432)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G08050
fragaria_vesca FvH4_5g13740
malus_domestica MD06G1225900.v1.1 MD14G1212800.v1.1
prunus_persica Prupe.5G206100_v2.0.a1
pyrus_communis pycom06g18030 pycom14g17640
rosa_chinensis RchiOBHm_Chr7g0183201
rosa_laevigata RLG00000005067
rosa_multiflora Rmu_sc0006717.1_g000006 Rmu_ssc0000111.1_g000019
rosa_roxburghii Rroxscaffold_3G00270660
rosa_rugosa Rorug06G0454800
rosa_samantha Rh7AG057200 Rh7BG056900 Rh7CG058000 Rh7DG056800
rosa_wichuraiana Rw7G004670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 115
AccBSI CCGCTC 1 cut(s) 56
AccI GTMKAC 1 cut(s) 275
AciI CCGC 7 cut(s) 56, 59, 62, 65, 224, 308, 332
AcoI YGGCCR 1 cut(s) 333
AcsI RAATTY 2 cut(s) 169, 314
AcuI CTGAAG 2 cut(s) 123, 227
AfiI CCNNNNNNNGG 2 cut(s) 115, 331
AflII CTTAAG 1 cut(s) 370
AjnI CCWGG 1 cut(s) 343
AlwNI CAGNNNCTG 1 cut(s) 476
Ama87I CYCGRG 1 cut(s) 353
AoxI GGCC 3 cut(s) 6, 235, 333
ApeKI GCWGC 5 cut(s) 21, 24, 467, 488, 497
ApoI RAATTY 2 cut(s) 169, 314
AspLEI GCGC 1 cut(s) 29
AspS9I GGNCC 1 cut(s) 395
AsuC2I CCSGG 3 cut(s) 262, 354, 355
AvaI CYCGRG 1 cut(s) 353
AvaII GGWCC 1 cut(s) 395
AxyI CCTNAGG 1 cut(s) 420
BanII GRGCYC 1 cut(s) 267
BarI GAAGNNNNNNTAC 2 cut(s) 70, 102
BbvI GCAGC 5 cut(s) 33, 36, 454, 475, 484
BceAI ACGGC 1 cut(s) 264
BciT130I CCWGG 1 cut(s) 345
BciVI GTATCC 1 cut(s) 523
BcnI CCSGG 3 cut(s) 262, 354, 355
BfaI CTAG 1 cut(s) 290
BfmI CTRYAG 1 cut(s) 468
BfrI CTTAAG 1 cut(s) 370
BfuI GTATCC 1 cut(s) 523
BlpI GCTNAGC 1 cut(s) 365
Bme1390I CCNGG 4 cut(s) 262, 345, 354, 355
Bme18I GGWCC 1 cut(s) 395
BmeT110I CYCGRG 1 cut(s) 353
BmgT120I GGNCC 1 cut(s) 395
BmiI GGNNCC 1 cut(s) 396
BmrFI CCNGG 4 cut(s) 262, 345, 354, 355
BmsI GCATC 1 cut(s) 509
Bpu10I CCTNAGC 1 cut(s) 376
Bpu1102I GCTNAGC 1 cut(s) 365
BpuMI CCSGG 3 cut(s) 262, 354, 355
BsaJI CCNNGG 6 cut(s) 114, 246, 343, 353, 354, 432
BsaXI ACNNNNNCTCC 2 cut(s) 428, 458
Bsc4I CCNNNNNNNGG 2 cut(s) 115, 331
Bse118I RCCGGY 1 cut(s) 233
Bse21I CCTNAGG 1 cut(s) 420
Bse3DI GCAATG 1 cut(s) 359
BseBI CCWGG 1 cut(s) 345
BseDI CCNNGG 6 cut(s) 114, 246, 343, 353, 354, 432
BseGI GGATG 1 cut(s) 105
BseLI CCNNNNNNNGG 2 cut(s) 115, 331
BseMI GCAATG 1 cut(s) 359
BseMII CTCAG 3 cut(s) 206, 356, 411
BseRI GAGGAG 1 cut(s) 92
BseXI GCAGC 5 cut(s) 33, 36, 454, 475, 484
BshFI GGCC 3 cut(s) 8, 237, 335
BsiHKCI CYCGRG 1 cut(s) 353
BsiSI CCGG 4 cut(s) 9, 234, 261, 354
BslI CCNNNNNNNGG 2 cut(s) 115, 331
BsnI GGCC 3 cut(s) 8, 237, 335
BsoBI CYCGRG 1 cut(s) 353
Bsp1286I GDGCHC 1 cut(s) 267
Bsp1720I GCTNAGC 1 cut(s) 365
Bsp19I CCATGG 1 cut(s) 114
BspACI CCGC 7 cut(s) 56, 59, 62, 65, 224, 308, 332
BspANI GGCC 3 cut(s) 8, 237, 335
BspCNI CTCAG 3 cut(s) 207, 357, 412
BspLI GGNNCC 1 cut(s) 396
BspMAI CTGCAG 1 cut(s) 472
BspTI CTTAAG 1 cut(s) 370
BsrBI CCGCTC 1 cut(s) 56
BsrDI GCAATG 1 cut(s) 359
BsrFI RCCGGY 1 cut(s) 233
BssAI RCCGGY 1 cut(s) 233
BssECI CCNNGG 6 cut(s) 114, 246, 343, 353, 354, 432
BssT1I CCWWGG 2 cut(s) 114, 432
Bst2UI CCWGG 1 cut(s) 345
BstAFI CTTAAG 1 cut(s) 370
BstC8I GCNNGC 4 cut(s) 38, 235, 239, 521
BstDEI CTNAG 5 cut(s) 155, 215, 365, 376, 420
BstDSI CCRYGG 2 cut(s) 114, 246
BstF5I GGATG 1 cut(s) 105
BstHHI GCGC 1 cut(s) 29
BstNI CCWGG 1 cut(s) 345
BstSCI CCNGG 4 cut(s) 260, 343, 352, 353
BstSFI CTRYAG 1 cut(s) 468
BstV1I GCAGC 5 cut(s) 33, 36, 454, 475, 484
Bsu36I CCTNAGG 1 cut(s) 420
BsuI GTATCC 1 cut(s) 523
BsuRI GGCC 3 cut(s) 8, 237, 335
BtgI CCRYGG 2 cut(s) 114, 246
BtsCI GGATG 1 cut(s) 105
Cac8I GCNNGC 4 cut(s) 38, 235, 239, 521
CaiI CAGNNNCTG 1 cut(s) 476
CfoI GCGC 1 cut(s) 29
Cfr10I RCCGGY 1 cut(s) 233
Cfr13I GGNCC 1 cut(s) 395
Cfr9I CCCGGG 1 cut(s) 353
CviAII CATG 2 cut(s) 115, 133
DdeI CTNAG 5 cut(s) 155, 215, 365, 376, 420
EaeI YGGCCR 1 cut(s) 333
Eco130I CCWWGG 2 cut(s) 114, 432
Eco24I GRGCYC 1 cut(s) 267
Eco47I GGWCC 1 cut(s) 395
Eco57I CTGAAG 2 cut(s) 123, 227
Eco81I CCTNAGG 1 cut(s) 420
Eco88I CYCGRG 1 cut(s) 353
EcoRI GAATTC 2 cut(s) 169, 314
EcoRII CCWGG 1 cut(s) 343
EcoT14I CCWWGG 2 cut(s) 114, 432
EcoT38I GRGCYC 1 cut(s) 267
ErhI CCWWGG 2 cut(s) 114, 432
FaeI CATG 2 cut(s) 118, 136
FaiI YATR 2 cut(s) 116, 134
FatI CATG 2 cut(s) 114, 132
FblI GTMKAC 1 cut(s) 275
FokI GGATG 1 cut(s) 92
FriOI GRGCYC 1 cut(s) 267
FspBI CTAG 1 cut(s) 290
GlaI GCGC 1 cut(s) 28
HaeIII GGCC 3 cut(s) 8, 237, 335
HapII CCGG 4 cut(s) 9, 234, 261, 354
HhaI GCGC 1 cut(s) 29
Hin1II CATG 2 cut(s) 118, 136
Hin6I GCGC 1 cut(s) 27
HinP1I GCGC 1 cut(s) 27
HincII GTYRAC 1 cut(s) 276
HindII GTYRAC 1 cut(s) 276
HindIII AAGCTT 1 cut(s) 373
HinfI GANTC 2 cut(s) 321, 425
HpaII CCGG 4 cut(s) 9, 234, 261, 354
Hpy166II GTNNAC 1 cut(s) 276
Hpy188I TCNGA 2 cut(s) 53, 320
Hpy8I GTNNAC 1 cut(s) 276
Hpy99I CGWCG 1 cut(s) 280
HpyAV CCTTC 1 cut(s) 147
HpyCH4V TGCA 5 cut(s) 21, 40, 470, 500, 523
HpyF3I CTNAG 5 cut(s) 155, 215, 365, 376, 420
Hsp92II CATG 2 cut(s) 118, 136
HspAI GCGC 1 cut(s) 27
KroI GCCGGC 1 cut(s) 233
KroNI GCCGGC 1 cut(s) 235
LmnI GCTCC 3 cut(s) 11, 164, 199
Lsp1109I GCAGC 5 cut(s) 33, 36, 454, 475, 484
LweI GCATC 1 cut(s) 509
MaeI CTAG 1 cut(s) 290
MaeIII GTNAC 2 cut(s) 77, 444
MbiI CCGCTC 1 cut(s) 56
MboII GAAGA 2 cut(s) 81, 178
MhlI GDGCHC 1 cut(s) 267
MluCI AATT 3 cut(s) 169, 314, 532
MnlI CCTC 6 cut(s) 26, 105, 110, 113, 304, 415
MroNI GCCGGC 1 cut(s) 233
MseI TTAA 1 cut(s) 371
MspA1I CMGCKG 2 cut(s) 332, 488
MspCI CTTAAG 1 cut(s) 370
MspI CCGG 4 cut(s) 9, 234, 261, 354
MspR9I CCNGG 4 cut(s) 262, 345, 354, 355
MvaI CCWGG 1 cut(s) 345
NaeI GCCGGC 1 cut(s) 235
NciI CCSGG 3 cut(s) 262, 354, 355
NcoI CCATGG 1 cut(s) 114
NgoMIV GCCGGC 1 cut(s) 233
NlaIII CATG 2 cut(s) 118, 136
NlaIV GGNNCC 1 cut(s) 396
PdiI GCCGGC 1 cut(s) 235
PfeI GAWTC 2 cut(s) 321, 425
PflMI CCANNNNNTGG 1 cut(s) 115
Psp6I CCWGG 1 cut(s) 343
PspGI CCWGG 1 cut(s) 343
PspN4I GGNNCC 1 cut(s) 396
PspPI GGNCC 1 cut(s) 395
PstI CTGCAG 1 cut(s) 472
PstNI CAGNNNCTG 1 cut(s) 476
PvuII CAGCTG 1 cut(s) 488
SalI GTCGAC 1 cut(s) 274
SaqAI TTAA 1 cut(s) 371
Sau96I GGNCC 1 cut(s) 395
ScrFI CCNGG 4 cut(s) 262, 345, 354, 355
SduI GDGCHC 1 cut(s) 267
SfaNI GCATC 1 cut(s) 509
SfcI CTRYAG 1 cut(s) 468
SinI GGWCC 1 cut(s) 395
SmaI CCCGGG 1 cut(s) 355
SmlI CTYRAG 1 cut(s) 370
SmoI CTYRAG 1 cut(s) 370
Sse9I AATT 3 cut(s) 169, 314, 532
SsiI CCGC 7 cut(s) 56, 59, 62, 65, 224, 308, 332
SspMI CTAG 1 cut(s) 290
StyD4I CCNGG 4 cut(s) 260, 343, 352, 353
StyI CCWWGG 2 cut(s) 114, 432
TaqI TCGA 1 cut(s) 275
TasI AATT 3 cut(s) 169, 314, 532
TauI GCSGC 5 cut(s) 59, 62, 65, 68, 335
TfiI GAWTC 2 cut(s) 321, 425
Tru1I TTAA 1 cut(s) 371
Tru9I TTAA 1 cut(s) 371
TseI GCWGC 5 cut(s) 21, 24, 467, 488, 497
TspDTI ATGAA 1 cut(s) 162
TspMI CCCGGG 1 cut(s) 353
Van91I CCANNNNNTGG 1 cut(s) 115
Vha464I CTTAAG 1 cut(s) 370
VpaK11BI GGWCC 1 cut(s) 395
XapI RAATTY 2 cut(s) 169, 314
XmaI CCCGGG 1 cut(s) 353
XmiI GTMKAC 1 cut(s) 275
XspI CTAG 1 cut(s) 290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.