Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0012623

Physical Location & Seq

Genomic Coordinates Forward (+)

35853138 .. 35855925

Gene Structure 2788 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1228700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2463 bp

ATGGCGAAGATGGGGCGGAATAACGCCAACAGAAGCCTCGAGAGTCTGTTGGACATGGACAAATCAGTTGCTACGAAAGTCTCTGGCCCTCCTCGACGCCTGAAGATGATTCCGGGCCATGGACTTGAGTTCAATAACCTTTCGTATAGTGTAATGAAGAAGCTGAAAAAAGACGGGGTTTGGATCGAAAAAGAAGCGTATCTTCTCAATGACATCTCTGGCCAGGCAGTGAGGGGTGAGATCATGGCCATCTTGGGACCTAGTGGTGCTGGGAAATCCACATTTCTTGATGCCTTGGCAGGACGGATTGCACAAGGGAGTTTGGAAGGCTCTGTTAGAATTGATGGAAGAACGGTTACTACAAGCTACATGAAGATGGTGTCGTCTTACGTGATGCAGGACGACCAGCTCTTTCCTATGCTGACTGTTTTCGAGACGTTCATGTTTGCAGCTGAGGTTAGGCTTCCTCCCTCCATTTCGAGGAATGAAAAGAGAAGGAGAGTCTATGAACTCCTTGAACAACTCGGCTTGCAGAGTGCAACACATACTTACATCGGAGATGAAGGGAGGAGAGGAGTGTCGGGAGGAGAAAGGCGGAGGGTGTCAATTGGAATTGACATCATCCACAAACCTTCACTGCTGTTTCTTGATGAACCAACTTCTGGTCTTGACTCCACAAGCGCTTTCAGTGTCGTGGAGAAGGTAAAGGAGATTGCTCGAGGTGGTAGCATCGTGATGATGACCATACATCAACCTTCATATCGTATTCAAATGCTCTTGGACCGCATGACAATCCTTGCAAGAGGAAGACTCGTATATTTGGGAAGCCCGCACGGTCTGTCTGCTCACCTTTCTGGATTTGGAAGGCCAGTTCCGGATGGCGAGAATGGCCTCGAGTATCTCCTGGACGTGATTAAGGAATATGATGAATCAACTGTGGGACTAGACCCGTTGGTGATGTACCAACGTGACGGGATCAAACCTGATCAGGTGGCCAGAACCCCAGTTTTAAAAACACCAAGGAGAACTCCTTACAATAAGACCCCTGCATCCAAGCACGCAATCAGCCTCCGTAGTCAAGCCTATTCCGTGGGAAACAGGACACCTGGACCAGGCCAATCTGGTCGGTTTGATTACGAGGATGCTGATGAGGACGTTGAGGATTTCGACAACTCCCTTGAAAGGAAATCAGTTCATATGACACCAAAGCATCATCGGAGCAGTGGTGTGTATAACCCTCGTCTAGCATCGCAGTTCTACAAGGACTTCCCTGTCTGGATATACCATGGAGTCAAAGGAACCCCTCGCCGCCCACCATCATGGACTCCAGTTAGAACTCCAGGAAAGACACCTGGACTGACGCCACTCAGAGTCTCGGGTATGAAGAATCAAGTTTCAAATCAGTATCCAACACCTCAACTCATACTGCAGCCACCAAGACGCGGTAGTACTAAGACCCCTGCACTCTTCAGCCCTTCCATTGACTCCTCCAATGCACCTTCTTACGAAGAATTTGAAATAGAAGACGAGCTTGATGAGCCTGACCTCGGGCCTAAATTTGCAAACCCGTGGCTCCGTGAGGTTGCAGTCCTCTCGTGGCGCACAGCGCTCAATGTGATTCGCACTCCTGAACTCTTCCTCTCGCGTGAGATTGTGTTGGCAGTCATGGCACTAATTCTGTCTTCCCTTTTCCGAAACTTGGGTGGTGATACTTTCACAGACATAAACCGTCTTCTCAACTTCTACATCTTTGCAGTCTGCCTGGTTTTCTTTTCGTCCAATGACGCTGTCCCGACTTTCATCCAAGAAAGGTTCATTTTCATCAGGGAAACTTCTCACAATGCTTATCGTGCTTCTTCCTACGTCATCTCTTCCCTCATTGTTTATCTCCCATTCTTTGCCATCCAAGGTTACACATTTGCTGCCATTACCAAATACATTCTTCGCCTCAAAAGCAATGTTTTCAACTTCTGGATCATCCTCTATGCCTCACTCATCACCACCAACGCTTACGTGATGCTTGTGAGCGCCCTTGTGCCGAGCTACATCACAGGGTATGCTGTTGTGATAGCTACCACAGCTCTTTTCTTTCTGACTTGCGGCTTTTTCTTGAAGCGCTCGAAGATACCGGAATACTGGAGGTGGGTGCATTACATCTCTGCAATCAAGTACCCATTTGAGGCAATGCTATCAAATGAATTCAAAGGAATACGTTGCTACCATGGAACGCCGGCAGATCTTTCCCCCGGACCTCTAGGAGATTTGAAGATCAGTAACCTGCACTTAACATCCCCATTAGTGGATAAGAACTGTGCGATCATCGGACAAGACGTACTCTCCACGATGGATATCAACAGAGATAACATCTGGTACAATGTCTTAATCTTGCTAGCTTGGGGTGTTCTCTACCGGATCTTATTCTACATGGTCCTCAGATTCTACTCCAAGAACCAGAGAAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005775 GO:0005886 GO:0006810 GO:0007034 GO:0007275 GO:0008150 GO:0009555 GO:0009653 GO:0009987 GO:0010208 GO:0010927 GO:0015399 GO:0015405 GO:0015893 GO:0016020 GO:0016021 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022607 GO:0022804 GO:0022857 GO:0030198 GO:0031224 GO:0031974 GO:0032501 GO:0032502 GO:0032989 GO:0042221 GO:0042493 GO:0042623 GO:0042626 GO:0042891 GO:0043062 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043492 GO:0044085 GO:0044422 GO:0044424 GO:0044425 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0045229 GO:0048229 GO:0048646 GO:0048856 GO:0048869 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0070013 GO:0071840 GO:0071944 GO:0085029

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ABC2_membrane ABC_tran

Protein Analysis

821

Amino Acids

92.31

Weight (kDa)

9.36

Isoelectric Point (pI)

43.25

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ABC_tran	PF00005	69 - 221	2.2e-29	ABC transporter
ABC2_membrane	PF01061	527 - 734	3.5e-39	ABC-2 type transporter

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012623)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_5g17530
malus_domestica	MD06G1228700.v1.1 MD14G1237900.v1.1
prunus_persica	Prupe.5G235100_v2.0.a1
pyrus_communis	pycom06g20500 pycom14g19900
rosa_chinensis	RchiOBHm_Chr3g0493261 RchiOBHm_Chr7g0178121
rosa_laevigata	RLG00000005469
rosa_multiflora	Rmu_sc0000425.1_g000011 Rmu_sc0005324.1_g000001
rosa_roxburghii	Rroxscaffold_6G00391680
rosa_rugosa	Rorug03G0260000 Rorug06G0413700
rosa_samantha	Rh3DG344200 Rh7CG012900 Rh7DG013200
rosa_wichuraiana	Rw3G027160 Rw7G001030

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1271
Acc36I	ACCTGC	1 cut(s)	2286
AccB7I	CCANNNNNTGG	1 cut(s)	662
AccII	CGCG	2 cut(s)	1443, 1645
AccIII	TCCGGA	1 cut(s)	874
AciI	CCGC	7 cut(s)	16, 595, 784, 830, 1309, 1443, 2100
AclWI	GGATC	4 cut(s)	191, 983, 1982, 2420
AcoI	YGGCCR	3 cut(s)	220, 246, 993
AcsI	RAATTY	3 cut(s)	1511, 1556, 2198
AcuI	CTGAAG	2 cut(s)	122, 1453
AcyI	GRCGYC	2 cut(s)	97, 1361
AfaI	GTAC	5 cut(s)	962, 1450, 2171, 2334, 2372
AfeI	AGCGCT	3 cut(s)	682, 1608, 2117
AjiI	CACGTC	1 cut(s)	910
AjnI	CCWGG	7 cut(s)	222, 903, 1105, 1111, 1339, 1351, 1761
AloI	GAACNNNNNNTCC	2 cut(s)	855, 887
AluBI	AGCT	9 cut(s)	163, 366, 409, 452, 1531, 2043, 2072, 2081, 2393
AluI	AGCT	9 cut(s)	163, 366, 409, 452, 1531, 2043, 2072, 2081, 2393
Alw26I	GTCTC	3 cut(s)	85, 428, 1378
AlwI	GGATC	4 cut(s)	191, 983, 1982, 2420
Ama87I	CYCGRG	5 cut(s)	38, 717, 893, 1375, 1547
Aor13HI	TCCGGA	1 cut(s)	874
Aor51HI	AGCGCT	3 cut(s)	682, 1608, 2117
AoxI	GGCC	9 cut(s)	85, 115, 220, 246, 866, 889, 993, 1114, 1550
ApeKI	GCWGC	3 cut(s)	449, 1429, 1922
ApoI	RAATTY	3 cut(s)	1511, 1556, 2198
AspLEI	GCGC	5 cut(s)	683, 1602, 1609, 2030, 2118
AspS9I	GGNCC	8 cut(s)	86, 115, 257, 781, 1109, 1550, 2249, 2428
AsuC2I	CCSGG	2 cut(s)	114, 2247
AsuHPI	GGTGA	5 cut(s)	248, 839, 967, 1718, 1990
AsuNHI	GCTAGC	1 cut(s)	2389
AvaI	CYCGRG	5 cut(s)	38, 717, 893, 1375, 1547
AvaII	GGWCC	5 cut(s)	257, 781, 1109, 2249, 2428
BalI	TGGCCA	3 cut(s)	222, 248, 995
BarI	GAAGNNNNNNTAC	2 cut(s)	340, 372
BauI	CACGAG	1 cut(s)	1594
BbsI	GAAGAC	4 cut(s)	814, 1530, 1674, 1724
BbvCI	CCTCAGC	1 cut(s)	453
BbvI	GCAGC	3 cut(s)	461, 1441, 1909
BccI	CCATC	8 cut(s)	4, 257, 338, 370, 872, 1324, 1910, 2338
BcgI	CGANNNNNNTGC	2 cut(s)	1575, 1609
BciT130I	CCWGG	7 cut(s)	224, 905, 1107, 1113, 1341, 1353, 1763
BciVI	GTATCC	1 cut(s)	1416
BclI	TGATCA	1 cut(s)	985
BcnI	CCSGG	2 cut(s)	114, 2247
BcoDI	GTCTC	3 cut(s)	85, 428, 1378
BfaI	CTAG	5 cut(s)	261, 944, 1244, 2255, 2390
BfmI	CTRYAG	1 cut(s)	1427
BfoI	RGCGCY	4 cut(s)	684, 1610, 2031, 2119
BfuAI	ACCTGC	1 cut(s)	2286
BfuI	GTATCC	1 cut(s)	1416
BglII	AGATCT	1 cut(s)	2236
BisI	GCNGC	5 cut(s)	450, 1309, 1430, 1923, 2101
BlsI	GCNGC	5 cut(s)	451, 1310, 1431, 1924, 2102
BmcAI	AGTACT	1 cut(s)	1450
Bme1390I	CCNGG	9 cut(s)	114, 224, 905, 1107, 1113, 1341, 1353, 1763, 2247
Bme18I	GGWCC	5 cut(s)	257, 781, 1109, 2249, 2428
BmeT110I	CYCGRG	5 cut(s)	38, 717, 893, 1375, 1547
BmgBI	CACGTC	1 cut(s)	910
BmgT120I	GGNCC	8 cut(s)	86, 115, 257, 781, 1109, 1550, 2249, 2428
BmiI	GGNNCC	3 cut(s)	258, 1300, 1574
BmrFI	CCNGG	9 cut(s)	114, 224, 905, 1107, 1113, 1341, 1353, 1763, 2247
BmrI	ACTGGG	1 cut(s)	998
BmsI	GCATC	8 cut(s)	280, 384, 738, 1058, 1132, 1219, 1256, 2007
BmtI	GCTAGC	1 cut(s)	2393
BmuI	ACTGGG	1 cut(s)	998
BpiI	GAAGAC	4 cut(s)	814, 1530, 1674, 1724
BplI	GAGNNNNNCTC	2 cut(s)	795, 827
BpmI	CTGGAG	3 cut(s)	1311, 1323, 2158
Bpu10I	CCTNAGC	1 cut(s)	453
BpuEI	CTTGAG	1 cut(s)	146
BpuMI	CCSGG	2 cut(s)	114, 2247
BsaAI	YACGTR	2 cut(s)	391, 2014
BsaBI	GATNNNNATC	1 cut(s)	2348
BsaHI	GRCGYC	2 cut(s)	97, 1361
BsaWI	WCCGGW	3 cut(s)	874, 2128, 2409
BsaXI	ACNNNNNCTCC	4 cut(s)	1210, 1240, 2321, 2351
Bse118I	RCCGGY	1 cut(s)	2230
Bse1I	ACTGG	4 cut(s)	869, 1004, 1328, 2141
Bse3DI	GCAATG	2 cut(s)	1963, 2190
Bse8I	GATNNNNATC	1 cut(s)	2348
BseAI	TCCGGA	1 cut(s)	874
BseBI	CCWGG	7 cut(s)	224, 905, 1107, 1113, 1341, 1353, 1763
BseGI	GGATG	8 cut(s)	621, 883, 1049, 1147, 1800, 1902, 1977, 2288
BseJI	GATNNNNATC	1 cut(s)	2348
BseMI	GCAATG	2 cut(s)	1963, 2190
BseMII	CTCAG	3 cut(s)	444, 1381, 2446
BseNI	ACTGG	4 cut(s)	869, 1004, 1328, 2141
BseRI	GAGGAG	5 cut(s)	81, 583, 588, 600, 1477
BseXI	GCAGC	3 cut(s)	461, 1441, 1909
BseYI	CCCAGC	1 cut(s)	269
BsgI	GTGCAG	2 cut(s)	1446, 2264
Bsh1236I	CGCG	2 cut(s)	1443, 1645
BshFI	GGCC	9 cut(s)	87, 117, 222, 248, 868, 891, 995, 1116, 1552
BsiHKCI	CYCGRG	5 cut(s)	38, 717, 893, 1375, 1547
BsiSI	CCGG	6 cut(s)	113, 875, 2129, 2231, 2247, 2410
BslFI	GGGAC	3 cut(s)	270, 954, 1775
BsmAI	GTCTC	3 cut(s)	85, 428, 1378
BsmBI	CGTCTC	1 cut(s)	428
BsmFI	GGGAC	3 cut(s)	270, 954, 1775
BsnI	GGCC	9 cut(s)	87, 117, 222, 248, 868, 891, 995, 1116, 1552
BsoBI	CYCGRG	5 cut(s)	38, 717, 893, 1375, 1547
Bsp13I	TCCGGA	1 cut(s)	874
Bsp143I	GATC	9 cut(s)	183, 240, 975, 985, 1974, 2236, 2268, 2316, 2412
Bsp19I	CCATGG	3 cut(s)	118, 1285, 2221
BspACI	CCGC	7 cut(s)	16, 595, 784, 830, 1309, 1443, 2100
BspANI	GGCC	9 cut(s)	87, 117, 222, 248, 868, 891, 995, 1116, 1552
BspCNI	CTCAG	3 cut(s)	445, 1380, 2445
BspEI	TCCGGA	1 cut(s)	874
BspFNI	CGCG	2 cut(s)	1443, 1645
BspLI	GGNNCC	3 cut(s)	258, 1300, 1574
BspMAI	CTGCAG	1 cut(s)	1431
BspMI	ACCTGC	1 cut(s)	2286
BspOI	GCTAGC	1 cut(s)	2393
BspPI	GGATC	4 cut(s)	191, 983, 1982, 2420
BsrDI	GCAATG	2 cut(s)	1963, 2190
BsrFI	RCCGGY	1 cut(s)	2230
BsrI	ACTGG	4 cut(s)	869, 1004, 1328, 2141
BssAI	RCCGGY	1 cut(s)	2230
BssMI	GATC	9 cut(s)	183, 240, 975, 985, 1974, 2236, 2268, 2316, 2412
BssNI	GRCGYC	2 cut(s)	97, 1361
BssSI	CACGAG	1 cut(s)	1594
BssT1I	CCWWGG	6 cut(s)	118, 294, 1019, 1285, 1906, 2221
Bst2BI	CACGAG	1 cut(s)	1594
Bst2UI	CCWGG	7 cut(s)	224, 905, 1107, 1113, 1341, 1353, 1763
Bst4CI	ACNGT	6 cut(s)	355, 427, 836, 937, 1730, 2312
Bst6I	CTCTTC	3 cut(s)	1472, 1640, 1876
BstACI	GRCGYC	2 cut(s)	97, 1361
BstBAI	YACGTR	2 cut(s)	391, 2014
BstC8I	GCNNGC	5 cut(s)	530, 830, 1059, 2232, 2391
BstDEI	CTNAG	4 cut(s)	453, 1367, 1452, 2432
BstDSI	CCRYGG	5 cut(s)	118, 1089, 1285, 1568, 2221
BstENI	CCTNNNNNAGG	1 cut(s)	1110
BstF5I	GGATG	8 cut(s)	621, 883, 1049, 1147, 1800, 1902, 1977, 2288
BstFNI	CGCG	2 cut(s)	1443, 1645
BstH2I	RGCGCY	4 cut(s)	684, 1610, 2031, 2119
BstHHI	GCGC	5 cut(s)	683, 1602, 1609, 2030, 2118
BstKTI	GATC	9 cut(s)	186, 243, 978, 988, 1977, 2239, 2271, 2319, 2415
BstMAI	GTCTC	3 cut(s)	85, 428, 1378
BstMBI	GATC	9 cut(s)	183, 240, 975, 985, 1974, 2236, 2268, 2316, 2412
BstMWI	GCNNNNNNNGC	7 cut(s)	888, 1537, 1606, 1667, 1850, 1953, 2078
BstNI	CCWGG	7 cut(s)	224, 905, 1107, 1113, 1341, 1353, 1763
BstSCI	CCNGG	9 cut(s)	112, 222, 903, 1105, 1111, 1339, 1351, 1761, 2245
BstSFI	CTRYAG	1 cut(s)	1427
BstUI	CGCG	2 cut(s)	1443, 1645
BstV1I	GCAGC	3 cut(s)	461, 1441, 1909
BstV2I	GAAGAC	4 cut(s)	814, 1530, 1674, 1724
BstX2I	RGATCY	2 cut(s)	2236, 2412
BstXI	CCANNNNNNTGG	1 cut(s)	1320
BstYI	RGATCY	2 cut(s)	2236, 2412
BsuI	GTATCC	1 cut(s)	1416
BsuRI	GGCC	9 cut(s)	87, 117, 222, 248, 868, 891, 995, 1116, 1552
BtgI	CCRYGG	5 cut(s)	118, 1089, 1285, 1568, 2221
BtgZI	GCGATG	1 cut(s)	1233
BtrI	CACGTC	1 cut(s)	910
BtsCI	GGATG	8 cut(s)	621, 883, 1049, 1147, 1800, 1902, 1977, 2288
BtsI	GCAGTG	3 cut(s)	234, 635, 1228
BtsIMutI	CAGTG	4 cut(s)	234, 635, 694, 1228
BveI	ACCTGC	1 cut(s)	2286
Cac8I	GCNNGC	5 cut(s)	530, 830, 1059, 2232, 2391
CfoI	GCGC	5 cut(s)	683, 1602, 1609, 2030, 2118
Cfr10I	RCCGGY	1 cut(s)	2230
Cfr13I	GGNCC	8 cut(s)	86, 115, 257, 781, 1109, 1550, 2249, 2428
CseI	GACGC	4 cut(s)	105, 1369, 1449, 1793
Csp6I	GTAC	5 cut(s)	961, 1449, 2170, 2333, 2371
CspCI	CAANNNNNGTGG	2 cut(s)	268, 303
CviQI	GTAC	5 cut(s)	961, 1449, 2170, 2333, 2371
DdeI	CTNAG	4 cut(s)	453, 1367, 1452, 2432
DpnI	GATC	9 cut(s)	185, 242, 977, 987, 1976, 2238, 2270, 2318, 2414
DpnII	GATC	9 cut(s)	183, 240, 975, 985, 1974, 2236, 2268, 2316, 2412
DraI	TTTAAA	1 cut(s)	1011
DrdI	GACNNNNNNGTC	1 cut(s)	1271
DseDI	GACNNNNNNGTC	1 cut(s)	1271
EaeI	YGGCCR	3 cut(s)	220, 246, 993
Eam1104I	CTCTTC	3 cut(s)	1472, 1640, 1876
EarI	CTCTTC	3 cut(s)	1472, 1640, 1876
EciI	GGCGGA	2 cut(s)	31, 610
Eco130I	CCWWGG	6 cut(s)	118, 294, 1019, 1285, 1906, 2221
Eco32I	GATATC	1 cut(s)	2350
Eco47I	GGWCC	5 cut(s)	257, 781, 1109, 2249, 2428
Eco47III	AGCGCT	3 cut(s)	682, 1608, 2117
Eco57I	CTGAAG	2 cut(s)	122, 1453
Eco88I	CYCGRG	5 cut(s)	38, 717, 893, 1375, 1547
EcoNI	CCTNNNNNAGG	1 cut(s)	1110
EcoO109I	RGGNCCY	1 cut(s)	257
EcoRI	GAATTC	1 cut(s)	2198
EcoRII	CCWGG	7 cut(s)	222, 903, 1105, 1111, 1339, 1351, 1761
EcoRV	GATATC	1 cut(s)	2350
EcoT14I	CCWWGG	6 cut(s)	118, 294, 1019, 1285, 1906, 2221
ErhI	CCWWGG	6 cut(s)	118, 294, 1019, 1285, 1906, 2221
Esp3I	CGTCTC	1 cut(s)	428
FalI	AAGNNNNNCTT	4 cut(s)	186, 218, 1515, 1547
FaqI	GGGAC	3 cut(s)	270, 954, 1775
FauI	CCCGC	1 cut(s)	837
FauNDI	CATATG	1 cut(s)	1197
FbaI	TGATCA	1 cut(s)	985
Fnu4HI	GCNGC	5 cut(s)	450, 1309, 1430, 1923, 2101
FokI	GGATG	8 cut(s)	608, 890, 1036, 1154, 1787, 1889, 1964, 2275
Fsp4HI	GCNGC	5 cut(s)	450, 1309, 1430, 1923, 2101
FspBI	CTAG	5 cut(s)	261, 944, 1244, 2255, 2390
GlaI	GCGC	5 cut(s)	682, 1601, 1608, 2029, 2117
GluI	GCNGC	5 cut(s)	450, 1309, 1430, 1923, 2101
GsaI	CCCAGC	1 cut(s)	273
GsuI	CTGGAG	3 cut(s)	1311, 1323, 2158
HaeII	RGCGCY	4 cut(s)	684, 1610, 2031, 2119
HaeIII	GGCC	9 cut(s)	87, 117, 222, 248, 868, 891, 995, 1116, 1552
HapII	CCGG	6 cut(s)	113, 875, 2129, 2231, 2247, 2410
HgaI	GACGC	4 cut(s)	105, 1369, 1449, 1793
HhaI	GCGC	5 cut(s)	683, 1602, 1609, 2030, 2118
Hin1I	GRCGYC	2 cut(s)	97, 1361
Hin6I	GCGC	5 cut(s)	681, 1600, 1607, 2028, 2116
HinP1I	GCGC	5 cut(s)	681, 1600, 1607, 2028, 2116
HpaII	CCGG	6 cut(s)	113, 875, 2129, 2231, 2247, 2410
HphI	GGTGA	5 cut(s)	248, 839, 967, 1718, 1990
Hpy188I	TCNGA	7 cut(s)	557, 1218, 1370, 1694, 2094, 2324, 2435
Hpy99I	CGWCG	1 cut(s)	99
HpyAV	CCTTC	9 cut(s)	320, 489, 557, 642, 694, 765, 858, 1485, 1509
HpyCH4III	ACNGT	6 cut(s)	355, 427, 836, 937, 1730, 2312
HpyCH4IV	ACGT	9 cut(s)	390, 437, 909, 967, 1155, 1863, 2013, 2212, 2331
HpyF10VI	GCNNNNNNNGC	7 cut(s)	888, 1537, 1606, 1667, 1850, 1953, 2078
HpyF3I	CTNAG	4 cut(s)	453, 1367, 1452, 2432
HpySE526I	ACGT	9 cut(s)	390, 437, 909, 967, 1155, 1863, 2013, 2212, 2331
Hsp92I	GRCGYC	2 cut(s)	97, 1361
HspAI	GCGC	5 cut(s)	681, 1600, 1607, 2028, 2116
Kpn2I	TCCGGA	1 cut(s)	874
KroI	GCCGGC	1 cut(s)	2230
KroNI	GCCGGC	1 cut(s)	2232
Ksp22I	TGATCA	1 cut(s)	985
Kzo9I	GATC	9 cut(s)	183, 240, 975, 985, 1974, 2236, 2268, 2316, 2412
LmnI	GCTCC	2 cut(s)	1218, 1578
Lsp1109I	GCAGC	3 cut(s)	461, 1441, 1909
LweI	GCATC	8 cut(s)	280, 384, 738, 1058, 1132, 1219, 1256, 2007
MaeI	CTAG	5 cut(s)	261, 944, 1244, 2255, 2390
MaeII	ACGT	9 cut(s)	390, 437, 909, 967, 1155, 1863, 2013, 2212, 2331
MaeIII	GTNAC	4 cut(s)	355, 968, 1910, 2273
MalI	GATC	9 cut(s)	185, 242, 977, 987, 1976, 2238, 2270, 2318, 2414
MboI	GATC	9 cut(s)	183, 240, 975, 985, 1974, 2236, 2268, 2316, 2412
MfeI	CAATTG	1 cut(s)	606
MflI	RGATCY	2 cut(s)	2236, 2412
MlsI	TGGCCA	3 cut(s)	222, 248, 995
MluCI	AATT	7 cut(s)	339, 606, 612, 1511, 1556, 1674, 2198
MluNI	TGGCCA	3 cut(s)	222, 248, 995
MlyI	GAGTC	8 cut(s)	52, 510, 665, 804, 1299, 1318, 1380, 1478
MmeI	TCCRAC	2 cut(s)	30, 1433
Mox20I	TGGCCA	3 cut(s)	222, 248, 995
MroI	TCCGGA	1 cut(s)	874
MroNI	GCCGGC	1 cut(s)	2230
MscI	TGGCCA	3 cut(s)	222, 248, 995
MseI	TTAA	4 cut(s)	915, 1010, 2285, 2381
MslI	CAYNNNNRTG	3 cut(s)	374, 734, 1318
Msp20I	TGGCCA	3 cut(s)	222, 248, 995
MspA1I	CMGCKG	1 cut(s)	452
MspI	CCGG	6 cut(s)	113, 875, 2129, 2231, 2247, 2410
MspR9I	CCNGG	9 cut(s)	114, 224, 905, 1107, 1113, 1341, 1353, 1763, 2247
MunI	CAATTG	1 cut(s)	606
MvaI	CCWGG	7 cut(s)	224, 905, 1107, 1113, 1341, 1353, 1763
MvnI	CGCG	2 cut(s)	1443, 1645
MwoI	GCNNNNNNNGC	7 cut(s)	888, 1537, 1606, 1667, 1850, 1953, 2078
NaeI	GCCGGC	1 cut(s)	2232
NciI	CCSGG	2 cut(s)	114, 2247
NcoI	CCATGG	3 cut(s)	118, 1285, 2221
NdeI	CATATG	1 cut(s)	1197
NdeII	GATC	9 cut(s)	183, 240, 975, 985, 1974, 2236, 2268, 2316, 2412
NgoMIV	GCCGGC	1 cut(s)	2230
NheI	GCTAGC	1 cut(s)	2389
NlaIV	GGNNCC	3 cut(s)	258, 1300, 1574
NmeAIII	GCCGAG	2 cut(s)	504, 2064
NmuCI	GTSAC	1 cut(s)	968
PaeR7I	CTCGAG	3 cut(s)	38, 717, 893
PcsI	WCGNNNNNNNCGW	1 cut(s)	2328
PdiI	GCCGGC	1 cut(s)	2232
PfeI	GAWTC	5 cut(s)	109, 929, 1387, 1618, 2436
PflFI	GACNNNGTC	1 cut(s)	1787
PflMI	CCANNNNNTGG	1 cut(s)	662
PfoI	TCCNGGA	2 cut(s)	903, 1339
PkrI	GCNGC	5 cut(s)	451, 1310, 1431, 1924, 2102
PleI	GAGTC	8 cut(s)	51, 509, 665, 804, 1298, 1318, 1379, 1478
PpsI	GAGTC	8 cut(s)	51, 509, 665, 804, 1298, 1318, 1379, 1478
Ppu21I	YACGTR	2 cut(s)	391, 2014
PpuMI	RGGWCCY	1 cut(s)	257
Psp5II	RGGWCCY	1 cut(s)	257
Psp6I	CCWGG	7 cut(s)	222, 903, 1105, 1111, 1339, 1351, 1761
PspFI	CCCAGC	1 cut(s)	269
PspGI	CCWGG	7 cut(s)	222, 903, 1105, 1111, 1339, 1351, 1761
PspN4I	GGNNCC	3 cut(s)	258, 1300, 1574
PspPI	GGNCC	8 cut(s)	86, 115, 257, 781, 1109, 1550, 2249, 2428
PspPPI	RGGWCCY	1 cut(s)	257
PspXI	VCTCGAGB	2 cut(s)	717, 893
PsrI	GAACNNNNNNTAC	2 cut(s)	343, 375
PstI	CTGCAG	1 cut(s)	1431
PsuI	RGATCY	2 cut(s)	2236, 2412
PsyI	GACNNNGTC	1 cut(s)	1787
PvuII	CAGCTG	1 cut(s)	452
RsaI	GTAC	5 cut(s)	962, 1450, 2171, 2334, 2372
RsaNI	GTAC	5 cut(s)	961, 1449, 2170, 2333, 2371
RseI	CAYNNNNRTG	3 cut(s)	374, 734, 1318
SaqAI	TTAA	4 cut(s)	915, 1010, 2285, 2381
SatI	GCNGC	5 cut(s)	450, 1309, 1430, 1923, 2101
Sau3AI	GATC	9 cut(s)	183, 240, 975, 985, 1974, 2236, 2268, 2316, 2412
Sau96I	GGNCC	8 cut(s)	86, 115, 257, 781, 1109, 1550, 2249, 2428
ScaI	AGTACT	1 cut(s)	1450
SchI	GAGTC	8 cut(s)	52, 510, 665, 804, 1299, 1318, 1380, 1478
ScrFI	CCNGG	9 cut(s)	114, 224, 905, 1107, 1113, 1341, 1353, 1763, 2247
SfaNI	GCATC	8 cut(s)	280, 384, 738, 1058, 1132, 1219, 1256, 2007
SfcI	CTRYAG	1 cut(s)	1427
Sfr274I	CTCGAG	3 cut(s)	38, 717, 893
SinI	GGWCC	5 cut(s)	257, 781, 1109, 2249, 2428
SlaI	CTCGAG	3 cut(s)	38, 717, 893
SmiMI	CAYNNNNRTG	3 cut(s)	374, 734, 1318
SmlI	CTYRAG	4 cut(s)	38, 125, 717, 893
SmoI	CTYRAG	4 cut(s)	38, 125, 717, 893
Sse9I	AATT	7 cut(s)	339, 606, 612, 1511, 1556, 1674, 2198
SsiI	CCGC	7 cut(s)	16, 595, 784, 830, 1309, 1443, 2100
SspMI	CTAG	5 cut(s)	261, 944, 1244, 2255, 2390
StyD4I	CCNGG	9 cut(s)	112, 222, 903, 1105, 1111, 1339, 1351, 1761, 2245
StyI	CCWWGG	6 cut(s)	118, 294, 1019, 1285, 1906, 2221
TaaI	ACNGT	6 cut(s)	355, 427, 836, 937, 1730, 2312
TaiI	ACGT	9 cut(s)	393, 440, 912, 970, 1158, 1866, 2016, 2215, 2334
TaqI	TCGA	9 cut(s)	39, 94, 186, 432, 479, 718, 894, 1167, 2120
TasI	AATT	7 cut(s)	339, 606, 612, 1511, 1556, 1674, 2198
TatI	WGTACW	1 cut(s)	1448
TauI	GCSGC	2 cut(s)	1311, 2103
TfiI	GAWTC	5 cut(s)	109, 929, 1387, 1618, 2436
Tru1I	TTAA	4 cut(s)	915, 1010, 2285, 2381
Tru9I	TTAA	4 cut(s)	915, 1010, 2285, 2381
TscAI	CASTG	4 cut(s)	234, 642, 694, 1228
TseFI	GTSAC	1 cut(s)	968
TseI	GCWGC	3 cut(s)	449, 1429, 1922
Tsp45I	GTSAC	1 cut(s)	968
TspGWI	ACGGA	4 cut(s)	319, 1061, 1078, 1565
TspRI	CASTG	4 cut(s)	234, 642, 694, 1228
Tth111I	GACNNNGTC	1 cut(s)	1787
Van91I	CCANNNNNTGG	1 cut(s)	662
VpaK11BI	GGWCC	5 cut(s)	257, 781, 1109, 2249, 2428
XagI	CCTNNNNNAGG	1 cut(s)	1110
XapI	RAATTY	3 cut(s)	1511, 1556, 2198
XhoI	CTCGAG	3 cut(s)	38, 717, 893
XspI	CTAG	5 cut(s)	261, 944, 1244, 2255, 2390
ZrmI	AGTACT	1 cut(s)	1450

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.