MD06G1230500.v1.1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0010425
Physical Location & Seq
Forward (+)
36010564 .. 36017735
7172 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1230500.v1.1.491

Sequence Viewer

Length: 3090 bp
ATGAAGAACCTTATTAAGAAACTGCATAACATGTCCAACCAATCAAAAGATTCAGAAGGGTCTGCAGCTTCATCAAGGGGCAGTAAGTCCATTAGCAAGTCGTCCTCTGAAAAGGAAAGGCTTTTGCACTCAAGGTCCCATCAGGGTTCTGAGCATAAACCCTTTTCGGGGATTTCGGGTTGGTTGAATTCAGTTGGCAATAGGCATACCCCTAGTCCCCCATCATCTTCAAATGTCAATAGGGCCGAGAGAATGGAGCAGCCACCTGATGCAGCTGTTAGTGGGAGTGGTTTAGATGTTGTTTCCGATACAGGTAAGCGCGACTCGGGGTCTAGTACCTCAAGGGATGCTGATATAACAGAAGAGTATCAGATTCAATTGGCTTTGGAGTTGAGTGCTAGGGAAGATCCAGAGGCAGTTCAGATTGAGGCTGTTAAGCAGATTAGCTTGGGGTCTAGTGCTCCTGATAATACTCCAGCTGAAGTTATAGCATACCGCTATTGGAATTACAATGCTCTTAGCTATGATGACAAGGTCGTGGATGGGTTCTATGATCTGTATGGAATATTGACGGAGTCAACCTCAGAAAGAATGCCTTCCCTTGTTGATCTGCAAGGAGCACCCTTGTCAGATAGTGTCACCTGGGAAGCAATTTTGGTCAATCGAGCTGCCGATGCAAATTTGTTGAAACTTGAACAGGTGGCCCTAGAAATGGCTGTAAAGTCAAGTTCAGATCCTCGGGTTTTTCTAAACAGCAATCTGGTGCAAAAACTTGCTCTTTTAGTTGCTGACTACATGGGTGGACCAGTTGTGAATCCTGAAAACATGTTGAGAGAATGGCAAAATCTTAGTTACAGTTTGAAAGCAAACAATGGTAGCATGGTTTTGCCACTGGGTTCTCTGAGGATTGGATTAGCTCGACATCGTGCATTGTTGTTCAAGGTTTTGGCTGATAGTGTGAGCATCCCATGCCGGTTGGTGAAAGGACAACAATACACGGGGTCCAGTGATGTTGCAATGAACTTTGTAAAAATTTATGATAGGGAGTATATTGTTGATCTGATGGCAGACCCCGGCACACTTATTCCATCTGATGCAGCTGGATCACATATAGAATATGACGAATCTTACTTTTCCGCCAGTCCTTTTTCTAGAGACATTGATTCCTCTCATGTTGCTTCTTCCAGTAGTGGAGTTGGGAGTTCGTATGAAGAACATTCGGACTTTGGAAAATTAGAGAAGAAATCCAGGTTAAGAAGTTTTGCTTCTTCCACAAGGGATTCCGAAGAGAGAGAAAAACCAATATCTCATGCAAATTTACCCAGACCAACTGAATGCGAGGAACAACCCAAGATGCCTTCAGATGAATTTAGATATGCCTCTGACATAGAAAAGATGCTGGTGCAGGAACTTCCAGGAAAGCCAAATTATCCTTTTGCGCATGCAAGATCCCCTTCATGGACTGAAGGTGTTAGCTCTCCAGCCGTTCATAAAAAGAAGGTTAAGGACGTTTCCAAATACATGATTGTGGCTGCCAAGGAGAATCCAAATCTAGCTCAAAAGCTTCATGATGTTTTACTTGAAAGTGGAGTTGTTGCTCCCCCAAACCTGTTTAGGGAAATATATCCAGAGGAGTTAGATGTGTCAACAGTTGAGACCAAGCGCCAGGCAGAAGATAAGAGCGAACATAAAGAAAGGTTTGGAACACAAAAAGCTAAAAGTCAAGATGATAAAAGGCCAGCTCACTCTCTGCCCCCTTTGCCTCAGCAAAGGGTGAATCTTAAAACTAGTACTTCTTGTCAACCGGAGATTCTTAAGCCTGTGGAAGGTTTAGGGGTCAGCCTTCCACTTGATACGAGGGAAGTAACTGGCCAGAATATCTCATCACAGTCTGAAGCATCTCCAGTTAAACATACAAAGAATGTCCCTGTTGCTGCAGCAGCAGCGGCCGCAGCAGCTGTTGTTGCATCTTCTATGGTAGTAGCTGCAGCAAAGTCAAGTACCGACCCAACTCTTGAACTTCCTGTGGCAGCTGCTGCCACTGCTACTGCTGCAGCTGTGGTTGCAACAACTGCAGCTGTCAATAAGCAATGTGATCAGGGTATGAGAAGTGATGGAGATGCAGAAAGTGGTGGTTTTGAACCAAGGGGCAGTGGTGGCCGTGAACATGATGCTTTGGGAGTTAGTTCAGAAGGCGAGCGAACATCAGATCGATCAGCAGGTAATGAAAGCACTAGATCTGACGTCACAATTGATGATGTTGCAGACTGCGAGATTCCTTTTGAGGATATCACCTTGGGTGAACGTATTGGACTTGGATCGTATGGGGAGGTCTATCATGGAGACTGGCATGGGACTGAGGTTGCTGTGAAGAGGTTTCTAGACCAAAACTTTTTGGGCGAATCACTTGATGAATTCAGAAGTGAGGTTCGGATAATGAAAAGACTCAGGCATCCTAATGTTGTTCTCTTCATGGGTGCAATAACTCGCCCTCCAAATCTTTCCATTGTTACAGAATTTCTTCCAAGAGGTAGTTTATACAGGTTATTACACCGGCCTAACAATCAATTAGATGAGCGAAGGCGCTTGAGGATGGCTCTTGATGCGGCTCGCGGAATGAATTACTTGCATAACTGCACTCCAGTAATTGTACACCGTGATTTGAAGTCTCCAAATCTTCTTGTCGACAAGAATTGGGTTGTGAAGGTCTGCGATTTTGGGTTATCACGAATGAAGAACAGCACATTTCTCTCATCAAGGTCGACTGCAGGGACGGCTGAATGGATGGCTCCGGAAGTGCTACGAAATGAACCTTCCGATGAAAAATGCGATGTTTATAGCTACGGGGTCATATTATGGGAGCTCTCTACGATGCAACAGCCATGGGGGGGAATGAACCCAATGCAAGTTGTTGGTGCAGTTGGATTTCAACATCGCCGTCTTGATATTCCAGATAATATAGATCCTGCAGTTGCAGATCTCATTAGAAAATGCTGGCAAACAGATCCAAAATTGAGACCGTCGTTTGCTGAGATCATGGCTACTCTGAAACCGTTGCAGAAGCCGATGAGCAGAAGTGCACAGACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009651 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0035556 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EDR1 Pkinase_Tyr

Protein Analysis

1030

Amino Acids

112.78

Weight (kDa)

5.83

Isoelectric Point (pI)

50.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EDR1_CTR1_ARMC3_pept PF14381 160 - 362 1.4e-74 EDR1/CTR1/ARMC3, peptidase-like
PK_Tyr_Ser-Thr PF07714 764 - 1016 1.3e-66 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 766 - 1014 7.5e-53 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 2247
Acc16I TGCGCA 1 cut(s) 1440
Acc36I ACCTGC 1 cut(s) 2210
AccI GTMKAC 2 cut(s) 2685, 2762
AccII CGCG 2 cut(s) 321, 2613
AccIII TCCGGA 1 cut(s) 2791
AciI CCGC 6 cut(s) 496, 1137, 1946, 1950, 2606, 2613
AclWI GGATC 7 cut(s) 401, 728, 1111, 1443, 2326, 2957, 2999
AcoI YGGCCR 3 cut(s) 1870, 1947, 2158
AcsI RAATTY 7 cut(s) 187, 679, 1032, 1315, 1367, 2414, 2516
AcuI CTGAAG 4 cut(s) 501, 1344, 1485, 1914
AcyI GRCGYC 1 cut(s) 2244
AfaI GTAC 4 cut(s) 337, 1792, 2002, 2652
AfiI CCNNNNNNNGG 7 cut(s) 167, 168, 712, 1458, 1615, 1826, 2888
AflII CTTAAG 1 cut(s) 1814
AflIII ACRYGT 2 cut(s) 30, 825
AhdI GACNNNNNGTC 1 cut(s) 328
AhlI ACTAGT 1 cut(s) 1787
AjnI CCWGG 4 cut(s) 641, 1247, 1414, 1665
Alw21I GWGCWC 4 cut(s) 463, 622, 2865, 3082
Alw26I GTCTC 5 cut(s) 1149, 1649, 2337, 2673, 3010
Alw44I GTGCAC 1 cut(s) 3078
AlwI GGATC 7 cut(s) 401, 728, 1111, 1443, 2326, 2957, 2999
AlwNI CAGNNNCTG 2 cut(s) 1958, 2036
Ama87I CYCGRG 2 cut(s) 325, 738
Aor13HI TCCGGA 1 cut(s) 2791
AoxI GGCC 7 cut(s) 243, 702, 1736, 1870, 1947, 2158, 2555
ApaLI GTGCAC 1 cut(s) 3078
ApoI RAATTY 7 cut(s) 187, 679, 1032, 1315, 1367, 2414, 2516
Asp700I GAANNNNTTC 2 cut(s) 595, 2520
AspLEI GCGC 4 cut(s) 321, 1441, 1665, 2586
AspS9I GGNCC 5 cut(s) 135, 243, 703, 803, 1002
AsuC2I CCSGG 1 cut(s) 1074
AsuHPI GGTGA 5 cut(s) 631, 991, 1786, 2284, 2312
AvaI CYCGRG 2 cut(s) 325, 738
AvaII GGWCC 3 cut(s) 135, 803, 1002
BaeGI GKGCMC 1 cut(s) 3082
BalI TGGCCA 1 cut(s) 1872
BanII GRGCYC 1 cut(s) 2865
Bbv12I GWGCWC 4 cut(s) 463, 622, 2865, 3082
BbvCI CCTCAGC 1 cut(s) 1764
BccI CCATC 8 cut(s) 147, 229, 536, 1057, 1096, 2108, 2587, 2779
BceAI ACGGC 4 cut(s) 1469, 2145, 2790, 2922
BciT130I CCWGG 4 cut(s) 643, 1249, 1416, 1667
BclI TGATCA 1 cut(s) 2095
BcnI CCSGG 1 cut(s) 1074
BcoDI GTCTC 5 cut(s) 1149, 1649, 2337, 2673, 3010
BcuI ACTAGT 1 cut(s) 1787
BfmI CTRYAG 7 cut(s) 63, 1935, 1986, 2052, 2073, 2766, 2967
BfoI RGCGCY 2 cut(s) 1666, 2587
BfrI CTTAAG 1 cut(s) 1814
BfuAI ACCTGC 1 cut(s) 2210
BglII AGATCT 2 cut(s) 2237, 2977
BmcAI AGTACT 1 cut(s) 1792
Bme1390I CCNGG 5 cut(s) 643, 1074, 1249, 1416, 1667
Bme18I GGWCC 3 cut(s) 135, 803, 1002
BmeRI GACNNNNNGTC 1 cut(s) 328
BmeT110I CYCGRG 2 cut(s) 325, 738
BmgT120I GGNCC 5 cut(s) 135, 243, 703, 803, 1002
BmiI GGNNCC 3 cut(s) 137, 1003, 2790
BmrFI CCNGG 5 cut(s) 643, 1074, 1249, 1416, 1667
BmrI ACTGGG 1 cut(s) 902
BmuI ACTGGG 1 cut(s) 902
BplI GAGNNNNNCTC 4 cut(s) 566, 598, 2581, 2613
BpmI CTGGAG 4 cut(s) 459, 1464, 1887, 2625
Bpu10I CCTNAGC 1 cut(s) 1764
BpuEI CTTGAG 3 cut(s) 115, 325, 2608
BpuMI CCSGG 1 cut(s) 1074
Bsa29I ATCGAT 1 cut(s) 2212
BsaHI GRCGYC 1 cut(s) 2244
BsaI GGTCTC 2 cut(s) 1649, 3010
BsaJI CCNNGG 7 cut(s) 642, 737, 1072, 1536, 2144, 2295, 2882
BsaWI WCCGGW 2 cut(s) 1804, 2791
BsaXI ACNNNNNCTCC 2 cut(s) 2476, 2506
Bsc4I CCNNNNNNNGG 7 cut(s) 167, 168, 712, 1458, 1615, 1826, 2888
Bse118I RCCGGY 2 cut(s) 972, 2553
Bse1I ACTGG 9 cut(s) 806, 897, 1005, 1140, 1185, 1873, 1904, 2351, 2642
Bse3DI GCAATG 2 cut(s) 1023, 2096
BseAI TCCGGA 1 cut(s) 2791
BseBI CCWGG 4 cut(s) 643, 1249, 1416, 1667
BseCI ATCGAT 1 cut(s) 2212
BseDI CCNNGG 7 cut(s) 642, 737, 1072, 1536, 2144, 2295, 2882
BseGI GGATG 6 cut(s) 352, 547, 963, 2452, 2598, 2790
BseLI CCNNNNNNNGG 7 cut(s) 167, 168, 712, 1458, 1615, 1826, 2888
BseMI GCAATG 2 cut(s) 1023, 2096
BseMII CTCAG 7 cut(s) 141, 597, 893, 1778, 2349, 2461, 3021
BseNI ACTGG 9 cut(s) 806, 897, 1005, 1140, 1185, 1873, 1904, 2351, 2642
BseRI GAGGAG 1 cut(s) 1646
BseSI GKGCMC 1 cut(s) 3082
BseX3I CGGCCG 1 cut(s) 1947
BsgI GTGCAG 3 cut(s) 1424, 2620, 2937
Bsh1236I CGCG 2 cut(s) 321, 2613
Bsh1285I CGRYCG 1 cut(s) 1950
BshFI GGCC 7 cut(s) 245, 704, 1738, 1872, 1949, 2160, 2557
BshVI ATCGAT 1 cut(s) 2212
BsiEI CGRYCG 1 cut(s) 1950
BsiHKAI GWGCWC 4 cut(s) 463, 622, 2865, 3082
BsiHKCI CYCGRG 2 cut(s) 325, 738
BsiSI CCGG 5 cut(s) 973, 1074, 1805, 2554, 2792
BslFI GGGAC 5 cut(s) 121, 201, 1910, 2368, 2785
BslI CCNNNNNNNGG 7 cut(s) 167, 168, 712, 1458, 1615, 1826, 2888
BsmAI GTCTC 5 cut(s) 1149, 1649, 2337, 2673, 3010
BsmFI GGGAC 5 cut(s) 121, 201, 1910, 2368, 2785
BsmI GAATGC 2 cut(s) 597, 1340
BsnI GGCC 7 cut(s) 245, 704, 1738, 1872, 1949, 2160, 2557
Bso31I GGTCTC 2 cut(s) 1649, 3010
BsoBI CYCGRG 2 cut(s) 325, 738
Bsp1286I GDGCHC 4 cut(s) 463, 622, 2865, 3082
Bsp13I TCCGGA 1 cut(s) 2791
Bsp1407I TGTACA 1 cut(s) 2650
Bsp19I CCATGG 1 cut(s) 2882
BspACI CCGC 6 cut(s) 496, 1137, 1946, 1950, 2606, 2613
BspANI GGCC 7 cut(s) 245, 704, 1738, 1872, 1949, 2160, 2557
BspCNI CTCAG 7 cut(s) 142, 596, 894, 1777, 2350, 2460, 3022
BspDI ATCGAT 1 cut(s) 2212
BspEI TCCGGA 1 cut(s) 2791
BspFNI CGCG 2 cut(s) 321, 2613
BspHI TCATGA 1 cut(s) 1567
BspLI GGNNCC 3 cut(s) 137, 1003, 2790
BspMAI CTGCAG 7 cut(s) 67, 1939, 1990, 2056, 2077, 2770, 2971
BspMI ACCTGC 1 cut(s) 2210
BspPI GGATC 7 cut(s) 401, 728, 1111, 1443, 2326, 2957, 2999
BspTI CTTAAG 1 cut(s) 1814
BspTNI GGTCTC 2 cut(s) 1649, 3010
BsrDI GCAATG 2 cut(s) 1023, 2096
BsrFI RCCGGY 2 cut(s) 972, 2553
BsrGI TGTACA 1 cut(s) 2650
BsrI ACTGG 9 cut(s) 806, 897, 1005, 1140, 1185, 1873, 1904, 2351, 2642
BssAI RCCGGY 2 cut(s) 972, 2553
BssECI CCNNGG 7 cut(s) 642, 737, 1072, 1536, 2144, 2295, 2882
BssNI GRCGYC 1 cut(s) 2244
BssT1I CCWWGG 4 cut(s) 1536, 2144, 2295, 2882
Bst2UI CCWGG 4 cut(s) 643, 1249, 1416, 1667
Bst4CI ACNGT 6 cut(s) 857, 1651, 1890, 2657, 3021, 3054
Bst6I CTCTTC 4 cut(s) 357, 1281, 2366, 2474
BstACI GRCGYC 1 cut(s) 2244
BstAFI CTTAAG 1 cut(s) 1814
BstAPI GCANNNNNTGC 3 cut(s) 969, 2036, 2072
BstAUI TGTACA 1 cut(s) 2650
BstC8I GCNNGC 5 cut(s) 1443, 1740, 2198, 2611, 2996
BstDEI CTNAG 9 cut(s) 150, 518, 583, 848, 902, 1764, 2358, 2447, 3030
BstDSI CCRYGG 1 cut(s) 2882
BstENI CCTNNNNNAGG 1 cut(s) 1824
BstF5I GGATG 6 cut(s) 352, 547, 963, 2452, 2598, 2790
BstFNI CGCG 2 cut(s) 321, 2613
BstH2I RGCGCY 2 cut(s) 1666, 2587
BstHHI GCGC 4 cut(s) 321, 1441, 1665, 2586
BstMAI GTCTC 5 cut(s) 1149, 1649, 2337, 2673, 3010
BstMCI CGRYCG 1 cut(s) 1950
BstNI CCWGG 4 cut(s) 643, 1249, 1416, 1667
BstNSI RCATGY 3 cut(s) 34, 829, 1445
BstSCI CCNGG 5 cut(s) 641, 1072, 1247, 1414, 1665
BstSFI CTRYAG 7 cut(s) 63, 1935, 1986, 2052, 2073, 2766, 2967
BstSLI GKGCMC 1 cut(s) 3082
BstUI CGCG 2 cut(s) 321, 2613
BstX2I RGATCY 7 cut(s) 406, 733, 1448, 2237, 2962, 2977, 3004
BstYI RGATCY 7 cut(s) 406, 733, 1448, 2237, 2962, 2977, 3004
BstZI CGGCCG 1 cut(s) 1947
Bsu15I ATCGAT 1 cut(s) 2212
BsuRI GGCC 7 cut(s) 245, 704, 1738, 1872, 1949, 2160, 2557
BsuTUI ATCGAT 1 cut(s) 2212
BtgI CCRYGG 1 cut(s) 2882
BtgZI GCGATG 2 cut(s) 2844, 2918
BtsCI GGATG 6 cut(s) 352, 547, 963, 2452, 2598, 2790
BtsI GCAGTG 2 cut(s) 2040, 2158
BtsIMutI CAGTG 4 cut(s) 890, 1012, 2040, 2158
BveI ACCTGC 1 cut(s) 2210
Cac8I GCNNGC 5 cut(s) 1443, 1740, 2198, 2611, 2996
CaiI CAGNNNCTG 2 cut(s) 1958, 2036
CciI TCATGA 1 cut(s) 1567
CciNI GCGGCCGC 1 cut(s) 1947
CfoI GCGC 4 cut(s) 321, 1441, 1665, 2586
Cfr10I RCCGGY 2 cut(s) 972, 2553
Cfr13I GGNCC 5 cut(s) 135, 243, 703, 803, 1002
ClaI ATCGAT 1 cut(s) 2212
Csp6I GTAC 4 cut(s) 336, 1791, 2001, 2651
CviQI GTAC 4 cut(s) 336, 1791, 2001, 2651
DdeI CTNAG 9 cut(s) 150, 518, 583, 848, 902, 1764, 2358, 2447, 3030
DriI GACNNNNNGTC 1 cut(s) 328
EaeI YGGCCR 3 cut(s) 1870, 1947, 2158
EagI CGGCCG 1 cut(s) 1947
Eam1104I CTCTTC 4 cut(s) 357, 1281, 2366, 2474
Eam1105I GACNNNNNGTC 1 cut(s) 328
EarI CTCTTC 4 cut(s) 357, 1281, 2366, 2474
EciI GGCGGA 1 cut(s) 1126
Ecl136II GAGCTC 1 cut(s) 2863
EclXI CGGCCG 1 cut(s) 1947
Eco130I CCWWGG 4 cut(s) 1536, 2144, 2295, 2882
Eco24I GRGCYC 1 cut(s) 2865
Eco31I GGTCTC 2 cut(s) 1649, 3010
Eco32I GATATC 1 cut(s) 2290
Eco47I GGWCC 3 cut(s) 135, 803, 1002
Eco52I CGGCCG 1 cut(s) 1947
Eco53kI GAGCTC 1 cut(s) 2863
Eco57I CTGAAG 4 cut(s) 501, 1344, 1485, 1914
Eco88I CYCGRG 2 cut(s) 325, 738
EcoICRI GAGCTC 1 cut(s) 2863
EcoNI CCTNNNNNAGG 1 cut(s) 1824
EcoO109I RGGNCCY 1 cut(s) 135
EcoRI GAATTC 2 cut(s) 187, 2414
EcoRII CCWGG 4 cut(s) 641, 1247, 1414, 1665
EcoRV GATATC 1 cut(s) 2290
EcoT14I CCWWGG 4 cut(s) 1536, 2144, 2295, 2882
EcoT38I GRGCYC 1 cut(s) 2865
ErhI CCWWGG 4 cut(s) 1536, 2144, 2295, 2882
FalI AAGNNNNNCTT 6 cut(s) 580, 612, 1249, 1281, 1438, 1470
FaqI GGGAC 5 cut(s) 121, 201, 1910, 2368, 2785
FbaI TGATCA 1 cut(s) 2095
FblI GTMKAC 2 cut(s) 2685, 2762
FokI GGATG 6 cut(s) 359, 554, 950, 2439, 2605, 2797
FriOI GRGCYC 1 cut(s) 2865
FspI TGCGCA 1 cut(s) 1440
GlaI GCGC 4 cut(s) 320, 1440, 1664, 2585
GsuI CTGGAG 4 cut(s) 459, 1464, 1887, 2625
HaeII RGCGCY 2 cut(s) 1666, 2587
HaeIII GGCC 7 cut(s) 245, 704, 1738, 1872, 1949, 2160, 2557
HapII CCGG 5 cut(s) 973, 1074, 1805, 2554, 2792
HhaI GCGC 4 cut(s) 321, 1441, 1665, 2586
Hin1I GRCGYC 1 cut(s) 2244
Hin6I GCGC 4 cut(s) 319, 1439, 1663, 2584
HinP1I GCGC 4 cut(s) 319, 1439, 1663, 2584
HincII GTYRAC 5 cut(s) 579, 1647, 1802, 2686, 2763
HindII GTYRAC 5 cut(s) 579, 1647, 1802, 2686, 2763
HindIII AAGCTT 1 cut(s) 1562
HpaII CCGG 5 cut(s) 973, 1074, 1805, 2554, 2792
HphI GGTGA 5 cut(s) 631, 991, 1786, 2284, 2312
Hpy99I CGWCG 1 cut(s) 3025
HpyCH4III ACNGT 6 cut(s) 857, 1651, 1890, 2657, 3021, 3054
HpyCH4IV ACGT 3 cut(s) 1509, 2244, 2305
HpyF3I CTNAG 9 cut(s) 150, 518, 583, 848, 902, 1764, 2358, 2447, 3030
HpySE526I ACGT 3 cut(s) 1509, 2244, 2305
Hsp92I GRCGYC 1 cut(s) 2244
HspAI GCGC 4 cut(s) 319, 1439, 1663, 2584
Kpn2I TCCGGA 1 cut(s) 2791
Ksp22I TGATCA 1 cut(s) 2095
LmnI GCTCC 6 cut(s) 256, 466, 617, 1603, 2794, 2860
MaeII ACGT 3 cut(s) 1509, 2244, 2305
MaeIII GTNAC 5 cut(s) 637, 851, 1864, 2245, 2509
MfeI CAATTG 2 cut(s) 377, 2250
MflI RGATCY 7 cut(s) 406, 733, 1448, 2237, 2962, 2977, 3004
MhlI GDGCHC 4 cut(s) 463, 622, 2865, 3082
MlsI TGGCCA 1 cut(s) 1872
MluNI TGGCCA 1 cut(s) 1872
MlyI GAGTC 3 cut(s) 317, 584, 2439
MmeI TCCRAC 2 cut(s) 60, 2902
Mox20I TGGCCA 1 cut(s) 1872
MroI TCCGGA 1 cut(s) 2791
MroXI GAANNNNTTC 2 cut(s) 595, 2520
MscI TGGCCA 1 cut(s) 1872
MseI TTAA 7 cut(s) 15, 435, 1253, 1503, 1782, 1815, 1908
MslI CAYNNNNRTG 2 cut(s) 1526, 2457
Msp20I TGGCCA 1 cut(s) 1872
MspA1I CMGCKG 8 cut(s) 275, 479, 1100, 1946, 1958, 2033, 2057, 2078
MspCI CTTAAG 1 cut(s) 1814
MspI CCGG 5 cut(s) 973, 1074, 1805, 2554, 2792
MspR9I CCNGG 5 cut(s) 643, 1074, 1249, 1416, 1667
MunI CAATTG 2 cut(s) 377, 2250
Mva1269I GAATGC 2 cut(s) 597, 1340
MvaI CCWGG 4 cut(s) 643, 1249, 1416, 1667
MvnI CGCG 2 cut(s) 321, 2613
NciI CCSGG 1 cut(s) 1074
NcoI CCATGG 1 cut(s) 2882
NlaIV GGNNCC 3 cut(s) 137, 1003, 2790
NmeAIII GCCGAG 1 cut(s) 271
NmuCI GTSAC 2 cut(s) 637, 2245
NotI GCGGCCGC 1 cut(s) 1947
NsbI TGCGCA 1 cut(s) 1440
NspI RCATGY 3 cut(s) 34, 829, 1445
PaeI GCATGC 1 cut(s) 1445
PagI TCATGA 1 cut(s) 1567
PciI ACATGT 2 cut(s) 30, 825
PctI GAATGC 2 cut(s) 597, 1340
PdmI GAANNNNTTC 2 cut(s) 595, 2520
PflFI GACNNNGTC 2 cut(s) 533, 574
PfoI TCCNGGA 1 cut(s) 1414
PleI GAGTC 3 cut(s) 317, 583, 2439
PpsI GAGTC 3 cut(s) 317, 583, 2439
PpuMI RGGWCCY 1 cut(s) 135
PscI ACATGT 2 cut(s) 30, 825
Psp124BI GAGCTC 1 cut(s) 2865
Psp5II RGGWCCY 1 cut(s) 135
Psp6I CCWGG 4 cut(s) 641, 1247, 1414, 1665
PspGI CCWGG 4 cut(s) 641, 1247, 1414, 1665
PspN4I GGNNCC 3 cut(s) 137, 1003, 2790
PspPI GGNCC 5 cut(s) 135, 243, 703, 803, 1002
PspPPI RGGWCCY 1 cut(s) 135
PstI CTGCAG 7 cut(s) 67, 1939, 1990, 2056, 2077, 2770, 2971
PstNI CAGNNNCTG 2 cut(s) 1958, 2036
PsuI RGATCY 7 cut(s) 406, 733, 1448, 2237, 2962, 2977, 3004
PsyI GACNNNGTC 2 cut(s) 533, 574
PvuII CAGCTG 7 cut(s) 275, 479, 1100, 1958, 2033, 2057, 2078
RsaI GTAC 4 cut(s) 337, 1792, 2002, 2652
RsaNI GTAC 4 cut(s) 336, 1791, 2001, 2651
RseI CAYNNNNRTG 2 cut(s) 1526, 2457
SacI GAGCTC 1 cut(s) 2865
SalI GTCGAC 2 cut(s) 2684, 2761
SaqAI TTAA 7 cut(s) 15, 435, 1253, 1503, 1782, 1815, 1908
Sau96I GGNCC 5 cut(s) 135, 243, 703, 803, 1002
ScaI AGTACT 1 cut(s) 1792
SchI GAGTC 3 cut(s) 317, 584, 2439
ScrFI CCNGG 5 cut(s) 643, 1074, 1249, 1416, 1667
SduI GDGCHC 4 cut(s) 463, 622, 2865, 3082
SfcI CTRYAG 7 cut(s) 63, 1935, 1986, 2052, 2073, 2766, 2967
SinI GGWCC 3 cut(s) 135, 803, 1002
SmiMI CAYNNNNRTG 2 cut(s) 1526, 2457
SmlI CTYRAG 4 cut(s) 130, 340, 1814, 2587
SmoI CTYRAG 4 cut(s) 130, 340, 1814, 2587
SpeI ACTAGT 1 cut(s) 1787
SphI GCATGC 1 cut(s) 1445
SsiI CCGC 6 cut(s) 496, 1137, 1946, 1950, 2606, 2613
SspI AATATT 1 cut(s) 567
SstI GAGCTC 1 cut(s) 2865
StyD4I CCNGG 5 cut(s) 641, 1072, 1247, 1414, 1665
StyI CCWWGG 4 cut(s) 1536, 2144, 2295, 2882
TaaI ACNGT 6 cut(s) 857, 1651, 1890, 2657, 3021, 3054
TaiI ACGT 3 cut(s) 1512, 2247, 2308
TaqI TCGA 5 cut(s) 664, 919, 2212, 2685, 2762
TatI WGTACW 2 cut(s) 1790, 2650
TauI GCSGC 3 cut(s) 1949, 1952, 2609
Tru1I TTAA 7 cut(s) 15, 435, 1253, 1503, 1782, 1815, 1908
Tru9I TTAA 7 cut(s) 15, 435, 1253, 1503, 1782, 1815, 1908
TscAI CASTG 4 cut(s) 897, 1012, 2047, 2158
TseFI GTSAC 2 cut(s) 637, 2245
Tsp45I GTSAC 2 cut(s) 637, 2245
TspGWI ACGGA 1 cut(s) 587
TspRI CASTG 4 cut(s) 897, 1012, 2047, 2158
Tth111I GACNNNGTC 2 cut(s) 533, 574
Vha464I CTTAAG 1 cut(s) 1814
VneI GTGCAC 1 cut(s) 3078
VpaK11BI GGWCC 3 cut(s) 135, 803, 1002
XagI CCTNNNNNAGG 1 cut(s) 1824
XapI RAATTY 7 cut(s) 187, 679, 1032, 1315, 1367, 2414, 2516
XbaI TCTAGA 2 cut(s) 1151, 2380
XceI RCATGY 3 cut(s) 34, 829, 1445
XmiI GTMKAC 2 cut(s) 2685, 2762
XmnI GAANNNNTTC 2 cut(s) 595, 2520
ZraI GACGTC 1 cut(s) 2245
ZrmI AGTACT 1 cut(s) 1792
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.