pycom14g19610

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0010425
Physical Location & Seq
Forward (+)
20879769 .. 20885593
5825 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g19610.12

Sequence Viewer

Length: 3036 bp
ATGAAGAACCTTCTTAAGAAACTGCATATCATGTCCAACCAATCAGAAGGTTCAGAAGGGTCTGCAGCTTCATCAAGGGGCAGTAAGTCCATTAGCAAGTCATCCCCTGAAACCGAAAGGCTTTTGCACTCGAGGTCCCATCAGGGTTCTGAGCATAAACCCTTCTCGGGGATTTCGGGTTGGTTGAATTCAGTTGCCAATAAGCATGGCCCTAGTCCCCCATCATCTTCGAATGTCAATAGGGCAGCGAGAGTGGAGCAGCCACCTGATGCAGCTGTTAGTGGGAGTGGTTTAGATGTTGTTTCGGATACAGGTAGGCGCGATTCGTGGTCTAGTACCTCGAGGGATGCTGATATAGCAGAAGAGTGTCAGATTCAATTGGCTTTGGAATTGAGTGCTAGGGAGGATCCAGAGGCAGTTCAGATTGAGGCTGTTAAGCAGATTAGCTTGGGGTCTTGTGCTCCTGATAATACTCCAGCTGAAGTTATAGCATACCGCTACTGGAATTACAATGCTCTTAGCTATGATGACAAGGTCATGGATGGTTTCTATGATCTGTATGGAATATTGATGGAGTCAACCTCTGAAAGAATGCCTTCCCTTGTTGATCTGCAAGGAACACCTTTGTCAGATAGTGTCACCTGGGAAGCAGTTTTGGTCAATAGAGCTGCCGATGCGAATTTGTTGAAACTTGAACAGGTGGCCCTAGAAATGGCTGTAAAGTCAAATTCTGATCCTCTGGCTTTTCTAAACAGCAATCTGGTGCGAAAACTTGCTCTTTTAGTTGCTGACCGTATGGGTGGACCAGTTGCGAATCCTGAAAACATGTTGAGAGAATGGAAAAATCTTAGTTACAGTTTGAAAGGAACCATTGGTAGCATGGTTTTGCCACTCGGTTCTCTGACAATTGGATTAGCTCGACATCGTGCATTGTTGTTCAAGGTTTTGGCTGATAGTGTGAGCATCCCATGCCGGTTGGTGAAAGGACAACAATACACCGGGTCCAGTGATATGGCAATGAACTTTGTAAAAGTTGATGATAGGGAGTATATTGTTGATCTGATGGCCGACCCTGGCACACTAATTCCATCTGATGCAGCTGGATCACATATAGAATGTGATGACTCTTACTTTTCTGCTAGTCCTCTGTCTAGAGACATTGATTCCTCTCATGTTGCTTCTTCCAGTAGTGGAGTTGGGAGTTCGTTTGAAGATCATTCAGACTTTGGAACATTAGAGAAGAAATCCAGGTTAAGAAATTTTGCTTCTTCCACAAGGGATTCCGAAGAGAGAGAAGAACCAGCTTCTCGTGATCTACCCAGACCAACTGAATTCGAGGAACAACCCAAGATGCCTTCAGATGAATTTAGATATGCCTCTGACGTAGAAAAGATGCTGGTGCAGGAACTTCCAGAAAAGCCAAATTATCCTTTTGCACATGCGAGATCCCCTTCATGGACTCAAGGTGTTAGTTCTCCAGCCGTTAATAAAAAGCAAGTTAAGGATGTTTCTAAATACATGATTGTTGCTGCCAAGGAGAATCCAAATCTAGCTCAAAAGCTTCATGATGTTTTACGCGAGAGTGGTGTTGATGCTCCCCCGAACTTGTTTAGGGAAATATATCCAGAGGAGTTAGATGTGTCAACAGTCGAGACCAAACGCCGGGCAGAAGATAAGAACGAAAATAAAGAAAGGTTTGGAACACAAAAATTTAAAAGTCAAGATGATAAAAGGCCAGCTCACTTTCTGCCTCCTTTGCCTCAGCAAAGGGTGAATCTTAAAGCTAGTACTTCCGGTCAACCGGAGATTCTTAAGCCTGTGGAAGGTTTAAGGTTCAGCCTTCCACTTGATACGAGGGACGTAACTGGCCAGAATATCTCATCACAGTCCGAAGCATCTCCAGTTAAATATACAAATAATGTCCCTGTTGCTGCAGCAGCGGCAGCCGCAGCAGCTGTTGTTGCATCGTCTATGGTAGTCGCCGCAGCAAAAACAAGTACTGACACAAGTCTCGAACTTCCTGTGGCAGCTGCTGCCACTGCTACTGCTGCAGCTGTGGTTGCAACAACTGTAGCTGTCAGTAAGCAATATGAGCCGGGCATGAGAAGTGATGGAGATTCAGAAAGTGGTGGTTTTGAACCAAGGGGCAGTGGTGGCCGTGAGCATGATGCTTCGGGAGTTAATTCCGAAGGCGAGCAAATATCAGATCGATCAGCAGGTAATGAAAGCACTAAATCTGATGTCACAATTGATGATGTTGCAGACTGCGAGATTCCCTGGGAGGACATCACCTTGGGTGAACGATCGTATGGGGAGGTCTATCATGGAGACTGGCATGGGACTGAGGTTGCTGTGAAGAAGTTTCTAGACCAAGACTTATTGGGCGAATCACTTGATGAATTCAGAAGTGAGGTTCGGATGATGAAAAGACTCAGGCATCCTAATGTTGTTCTCTTCATGGGTGCAATAACTCGCGCTCCAAATCTTTCCATTGTTACAGAATTTCTTCCAAGAGGTAGTTTATACAGGTTATTACACCGGCCTAACAATCAATTAGATGAGCGGAGGCGCTTGAGGATGGCTCTTGATGCTGCTCGCGGAATGAATTACTTGCATAACTGCACTCCAGTAATTGTACACCGTGATTTGAAGTCTCCAAATCTTCTTGTCGATAAGAACTGGGTTGTGAAGGTCTGTGACTTTGGATTATCACGAATGAAGAACAGCACATTTCTCTCCTCGAGGTCGACTGCAGGGACGGCTGAATGGATGGCTCCGGAAGTGCTAAGAAATGAACCTTCGGATGAAAAATGCGATGTTTATAGCTACGGGGTCATTTTATGGGAGCTCTCTACGATGCAACAGCCATGGGGAGGAATGAACCCAATGCAAGTTGTTGGTGCAGTTGGATTTCAACATCGCCGTCTTGATATTCCAGATAATATAGATCCTGGAATTGTATCGATCTCATTCGAAAATGCTGGCAAACATCCAAAATTGAGACCGTCGTTTACTGATCGATCATGGCAATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009651 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0035556 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EDR1 Pkinase_Tyr

Protein Analysis

1012

Amino Acids

110.92

Weight (kDa)

5.42

Isoelectric Point (pI)

49.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 2 cut(s) 340, 2740
Acc36I ACCTGC 1 cut(s) 2207
AccBSI CCGCTC 1 cut(s) 2563
AccI GTMKAC 1 cut(s) 2747
AccII CGCG 4 cut(s) 321, 1578, 2475, 2598
AccIII TCCGGA 1 cut(s) 2776
AciI CCGC 6 cut(s) 496, 1940, 1947, 1983, 2563, 2598
AclWI GGATC 6 cut(s) 401, 414, 728, 1111, 1440, 2942
AcoI YGGCCR 3 cut(s) 1065, 1867, 2155
AcsI RAATTY 9 cut(s) 187, 679, 727, 1258, 1331, 1364, 1709, 2399, 2501
AcuI CTGAAG 2 cut(s) 501, 1341
AfaI GTAC 4 cut(s) 337, 1789, 1999, 2637
AfiI CCNNNNNNNGG 7 cut(s) 167, 168, 712, 1455, 1662, 1823, 2873
AflII CTTAAG 2 cut(s) 14, 1811
AflIII ACRYGT 1 cut(s) 825
AjnI CCWGG 5 cut(s) 641, 1072, 1247, 2276, 2950
Alw21I GWGCWC 2 cut(s) 463, 2850
Alw26I GTCTC 6 cut(s) 1149, 1646, 2015, 2322, 2658, 2995
AlwI GGATC 6 cut(s) 401, 414, 728, 1111, 1440, 2942
AlwNI CAGNNNCTG 2 cut(s) 1955, 2033
Ama87I CYCGRG 4 cut(s) 130, 166, 340, 2740
Aor13HI TCCGGA 1 cut(s) 2776
AoxI GGCC 7 cut(s) 208, 702, 1065, 1733, 1867, 2155, 2540
ApoI RAATTY 9 cut(s) 187, 679, 727, 1258, 1331, 1364, 1709, 2399, 2501
Asp700I GAANNNNTTC 2 cut(s) 595, 2505
AspLEI GCGC 3 cut(s) 321, 2477, 2571
AspS9I GGNCC 5 cut(s) 135, 209, 703, 803, 1002
AsuC2I CCSGG 3 cut(s) 1000, 1664, 2097
AsuHPI GGTGA 5 cut(s) 631, 991, 1783, 2281, 2309
AsuII TTCGAA 2 cut(s) 230, 2973
AvaI CYCGRG 4 cut(s) 130, 166, 340, 2740
AvaII GGWCC 3 cut(s) 135, 803, 1002
BalI TGGCCA 1 cut(s) 1869
BamHI GGATCC 1 cut(s) 406
BanII GRGCYC 1 cut(s) 2850
BauI CACGAG 1 cut(s) 1308
Bbv12I GWGCWC 2 cut(s) 463, 2850
BbvCI CCTCAGC 1 cut(s) 1761
BccI CCATC 9 cut(s) 147, 229, 536, 565, 1057, 1096, 2105, 2572, 2764
BceAI ACGGC 4 cut(s) 1466, 2142, 2775, 2907
BciT130I CCWGG 5 cut(s) 643, 1074, 1249, 2278, 2952
BciVI GTATCC 1 cut(s) 301
BcnI CCSGG 3 cut(s) 1000, 1664, 2097
BcoDI GTCTC 6 cut(s) 1149, 1646, 2015, 2322, 2658, 2995
BfaI CTAG 9 cut(s) 213, 333, 399, 707, 1140, 1152, 1550, 1785, 2366
BfmI CTRYAG 5 cut(s) 63, 1932, 2049, 2070, 2751
BfoI RGCGCY 1 cut(s) 2572
BfrI CTTAAG 2 cut(s) 14, 1811
BfuAI ACCTGC 1 cut(s) 2207
BfuI GTATCC 1 cut(s) 301
BmcAI AGTACT 2 cut(s) 1789, 1999
Bme1390I CCNGG 8 cut(s) 643, 1000, 1074, 1249, 1664, 2097, 2278, 2952
Bme18I GGWCC 3 cut(s) 135, 803, 1002
BmeT110I CYCGRG 4 cut(s) 130, 166, 340, 2740
BmgT120I GGNCC 5 cut(s) 135, 209, 703, 803, 1002
BmiI GGNNCC 5 cut(s) 137, 408, 868, 1003, 2775
BmrFI CCNGG 8 cut(s) 643, 1000, 1074, 1249, 1664, 2097, 2278, 2952
BmrI ACTGGG 1 cut(s) 2689
BmuI ACTGGG 1 cut(s) 2689
BoxI GACNNNNGTC 1 cut(s) 2007
BplI GAGNNNNNCTC 4 cut(s) 566, 598, 2566, 2598
BpmI CTGGAG 4 cut(s) 459, 1461, 1884, 2610
Bpu10I CCTNAGC 1 cut(s) 1761
Bpu14I TTCGAA 2 cut(s) 230, 2973
BpuEI CTTGAG 2 cut(s) 1446, 2593
BpuMI CCSGG 3 cut(s) 1000, 1664, 2097
Bsa29I ATCGAT 3 cut(s) 2209, 2963, 3019
BsaI GGTCTC 2 cut(s) 1646, 2995
BsaJI CCNNGG 8 cut(s) 642, 1072, 1533, 2141, 2276, 2277, 2292, 2867
BsaWI WCCGGW 3 cut(s) 1793, 1801, 2776
BsaXI ACNNNNNCTCC 2 cut(s) 2461, 2491
Bsc4I CCNNNNNNNGG 7 cut(s) 167, 168, 712, 1455, 1662, 1823, 2873
Bse118I RCCGGY 2 cut(s) 972, 2538
Bse1I ACTGG 9 cut(s) 506, 806, 1005, 1185, 1870, 1901, 2336, 2627, 2684
Bse3DI GCAATG 1 cut(s) 1023
BseAI TCCGGA 1 cut(s) 2776
BseBI CCWGG 5 cut(s) 643, 1074, 1249, 2278, 2952
BseCI ATCGAT 3 cut(s) 2209, 2963, 3019
BseDI CCNNGG 8 cut(s) 642, 1072, 1533, 2141, 2276, 2277, 2292, 2867
BseLI CCNNNNNNNGG 7 cut(s) 167, 168, 712, 1455, 1662, 1823, 2873
BseMI GCAATG 1 cut(s) 1023
BseMII CTCAG 4 cut(s) 141, 1775, 2334, 2446
BseNI ACTGG 9 cut(s) 506, 806, 1005, 1185, 1870, 1901, 2336, 2627, 2684
BseRI GAGGAG 2 cut(s) 1643, 2728
BsgI GTGCAG 3 cut(s) 1421, 2605, 2922
Bsh1236I CGCG 4 cut(s) 321, 1578, 2475, 2598
Bsh1285I CGRYCG 1 cut(s) 2306
BshFI GGCC 7 cut(s) 210, 704, 1067, 1735, 1869, 2157, 2542
BshVI ATCGAT 3 cut(s) 2209, 2963, 3019
BsiEI CGRYCG 1 cut(s) 2306
BsiHKAI GWGCWC 2 cut(s) 463, 2850
BsiHKCI CYCGRG 4 cut(s) 130, 166, 340, 2740
BsiSI CCGG 8 cut(s) 973, 999, 1663, 1794, 1802, 2096, 2539, 2777
BslFI GGGAC 6 cut(s) 121, 201, 1871, 1907, 2353, 2770
BslI CCNNNNNNNGG 7 cut(s) 167, 168, 712, 1455, 1662, 1823, 2873
BsmAI GTCTC 6 cut(s) 1149, 1646, 2015, 2322, 2658, 2995
BsmFI GGGAC 6 cut(s) 121, 201, 1871, 1907, 2353, 2770
BsmI GAATGC 1 cut(s) 597
BsnI GGCC 7 cut(s) 210, 704, 1067, 1735, 1869, 2157, 2542
Bso31I GGTCTC 2 cut(s) 1646, 2995
BsoBI CYCGRG 4 cut(s) 130, 166, 340, 2740
Bsp119I TTCGAA 2 cut(s) 230, 2973
Bsp1286I GDGCHC 2 cut(s) 463, 2850
Bsp13I TCCGGA 1 cut(s) 2776
Bsp1407I TGTACA 1 cut(s) 2635
Bsp19I CCATGG 1 cut(s) 2867
BspACI CCGC 6 cut(s) 496, 1940, 1947, 1983, 2563, 2598
BspANI GGCC 7 cut(s) 210, 704, 1067, 1735, 1869, 2157, 2542
BspCNI CTCAG 4 cut(s) 142, 1774, 2335, 2445
BspDI ATCGAT 3 cut(s) 2209, 2963, 3019
BspEI TCCGGA 1 cut(s) 2776
BspFNI CGCG 4 cut(s) 321, 1578, 2475, 2598
BspHI TCATGA 1 cut(s) 1564
BspLI GGNNCC 5 cut(s) 137, 408, 868, 1003, 2775
BspMAI CTGCAG 4 cut(s) 67, 1936, 2053, 2755
BspMI ACCTGC 1 cut(s) 2207
BspPI GGATC 6 cut(s) 401, 414, 728, 1111, 1440, 2942
BspT104I TTCGAA 2 cut(s) 230, 2973
BspTI CTTAAG 2 cut(s) 14, 1811
BspTNI GGTCTC 2 cut(s) 1646, 2995
BsrBI CCGCTC 1 cut(s) 2563
BsrDI GCAATG 1 cut(s) 1023
BsrFI RCCGGY 2 cut(s) 972, 2538
BsrGI TGTACA 1 cut(s) 2635
BsrI ACTGG 9 cut(s) 506, 806, 1005, 1185, 1870, 1901, 2336, 2627, 2684
BssAI RCCGGY 2 cut(s) 972, 2538
BssECI CCNNGG 8 cut(s) 642, 1072, 1533, 2141, 2276, 2277, 2292, 2867
BssSI CACGAG 1 cut(s) 1308
BssT1I CCWWGG 4 cut(s) 1533, 2141, 2292, 2867
Bst2BI CACGAG 1 cut(s) 1308
Bst2UI CCWGG 5 cut(s) 643, 1074, 1249, 2278, 2952
Bst4CI ACNGT 7 cut(s) 794, 857, 1648, 1887, 2071, 2642, 3006
Bst6I CTCTTC 3 cut(s) 357, 1281, 2459
BstAFI CTTAAG 2 cut(s) 14, 1811
BstAPI GCANNNNNTGC 2 cut(s) 969, 2033
BstAUI TGTACA 1 cut(s) 2635
BstBI TTCGAA 2 cut(s) 230, 2973
BstC8I GCNNGC 4 cut(s) 1737, 2195, 2596, 2983
BstDEI CTNAG 7 cut(s) 150, 518, 848, 1761, 2343, 2432, 2786
BstDSI CCRYGG 1 cut(s) 2867
BstENI CCTNNNNNAGG 1 cut(s) 1821
BstFNI CGCG 4 cut(s) 321, 1578, 2475, 2598
BstH2I RGCGCY 1 cut(s) 2572
BstHHI GCGC 3 cut(s) 321, 2477, 2571
BstMAI GTCTC 6 cut(s) 1149, 1646, 2015, 2322, 2658, 2995
BstMCI CGRYCG 1 cut(s) 2306
BstNI CCWGG 5 cut(s) 643, 1074, 1249, 2278, 2952
BstNSI RCATGY 2 cut(s) 829, 1442
BstPAI GACNNNNGTC 1 cut(s) 2007
BstSCI CCNGG 8 cut(s) 641, 998, 1072, 1247, 1662, 2095, 2276, 2950
BstSFI CTRYAG 5 cut(s) 63, 1932, 2049, 2070, 2751
BstUI CGCG 4 cut(s) 321, 1578, 2475, 2598
BstX2I RGATCY 3 cut(s) 406, 1445, 2947
BstXI CCANNNNNNTGG 1 cut(s) 1012
BstYI RGATCY 3 cut(s) 406, 1445, 2947
Bsu15I ATCGAT 3 cut(s) 2209, 2963, 3019
BsuI GTATCC 1 cut(s) 301
BsuRI GGCC 7 cut(s) 210, 704, 1067, 1735, 1869, 2157, 2542
BsuTUI ATCGAT 3 cut(s) 2209, 2963, 3019
BtgI CCRYGG 1 cut(s) 2867
BtgZI GCGATG 2 cut(s) 2829, 2903
BtsI GCAGTG 2 cut(s) 2037, 2155
BtsIMutI CAGTG 3 cut(s) 1012, 2037, 2155
BveI ACCTGC 1 cut(s) 2207
Cac8I GCNNGC 4 cut(s) 1737, 2195, 2596, 2983
CaiI CAGNNNCTG 2 cut(s) 1955, 2033
CciI TCATGA 1 cut(s) 1564
CfoI GCGC 3 cut(s) 321, 2477, 2571
Cfr10I RCCGGY 2 cut(s) 972, 2538
Cfr13I GGNCC 5 cut(s) 135, 209, 703, 803, 1002
ClaI ATCGAT 3 cut(s) 2209, 2963, 3019
Csp6I GTAC 4 cut(s) 336, 1788, 1998, 2636
CviQI GTAC 4 cut(s) 336, 1788, 1998, 2636
DdeI CTNAG 7 cut(s) 150, 518, 848, 1761, 2343, 2432, 2786
DraI TTTAAA 1 cut(s) 1714
EaeI YGGCCR 3 cut(s) 1065, 1867, 2155
Eam1104I CTCTTC 3 cut(s) 357, 1281, 2459
EarI CTCTTC 3 cut(s) 357, 1281, 2459
Ecl136II GAGCTC 1 cut(s) 2848
Eco130I CCWWGG 4 cut(s) 1533, 2141, 2292, 2867
Eco24I GRGCYC 1 cut(s) 2850
Eco31I GGTCTC 2 cut(s) 1646, 2995
Eco47I GGWCC 3 cut(s) 135, 803, 1002
Eco53kI GAGCTC 1 cut(s) 2848
Eco57I CTGAAG 2 cut(s) 501, 1341
Eco88I CYCGRG 4 cut(s) 130, 166, 340, 2740
EcoICRI GAGCTC 1 cut(s) 2848
EcoNI CCTNNNNNAGG 1 cut(s) 1821
EcoO109I RGGNCCY 1 cut(s) 135
EcoRI GAATTC 3 cut(s) 187, 1331, 2399
EcoRII CCWGG 5 cut(s) 641, 1072, 1247, 2276, 2950
EcoT14I CCWWGG 4 cut(s) 1533, 2141, 2292, 2867
EcoT38I GRGCYC 1 cut(s) 2850
ErhI CCWWGG 4 cut(s) 1533, 2141, 2292, 2867
FalI AAGNNNNNCTT 2 cut(s) 580, 612
FaqI GGGAC 6 cut(s) 121, 201, 1871, 1907, 2353, 2770
FblI GTMKAC 1 cut(s) 2747
FriOI GRGCYC 1 cut(s) 2850
FspBI CTAG 9 cut(s) 213, 333, 399, 707, 1140, 1152, 1550, 1785, 2366
GlaI GCGC 3 cut(s) 320, 2476, 2570
GsuI CTGGAG 4 cut(s) 459, 1461, 1884, 2610
HaeII RGCGCY 1 cut(s) 2572
HaeIII GGCC 7 cut(s) 210, 704, 1067, 1735, 1869, 2157, 2542
HapII CCGG 8 cut(s) 973, 999, 1663, 1794, 1802, 2096, 2539, 2777
HhaI GCGC 3 cut(s) 321, 2477, 2571
Hin6I GCGC 3 cut(s) 319, 2475, 2569
HinP1I GCGC 3 cut(s) 319, 2475, 2569
HincII GTYRAC 4 cut(s) 579, 1644, 1799, 2748
HindII GTYRAC 4 cut(s) 579, 1644, 1799, 2748
HindIII AAGCTT 1 cut(s) 1559
HpaII CCGG 8 cut(s) 973, 999, 1663, 1794, 1802, 2096, 2539, 2777
HphI GGTGA 5 cut(s) 631, 991, 1783, 2281, 2309
Hpy166II GTNNAC 8 cut(s) 579, 803, 1644, 1799, 2300, 2638, 2748, 3012
Hpy8I GTNNAC 8 cut(s) 579, 803, 1644, 1799, 2300, 2638, 2748, 3012
Hpy99I CGWCG 1 cut(s) 3010
HpyCH4III ACNGT 7 cut(s) 794, 857, 1648, 1887, 2071, 2642, 3006
HpyCH4IV ACGT 2 cut(s) 1383, 1860
HpyF3I CTNAG 7 cut(s) 150, 518, 848, 1761, 2343, 2432, 2786
HpySE526I ACGT 2 cut(s) 1383, 1860
HspAI GCGC 3 cut(s) 319, 2475, 2569
Kpn2I TCCGGA 1 cut(s) 2776
LmnI GCTCC 6 cut(s) 256, 466, 1600, 2482, 2779, 2845
MaeI CTAG 9 cut(s) 213, 333, 399, 707, 1140, 1152, 1550, 1785, 2366
MaeII ACGT 2 cut(s) 1383, 1860
MaeIII GTNAC 6 cut(s) 637, 851, 1861, 2242, 2494, 2696
MbiI CCGCTC 1 cut(s) 2563
MfeI CAATTG 3 cut(s) 377, 906, 2247
MflI RGATCY 3 cut(s) 406, 1445, 2947
MhlI GDGCHC 2 cut(s) 463, 2850
MlsI TGGCCA 1 cut(s) 1869
MluNI TGGCCA 1 cut(s) 1869
MlyI GAGTC 4 cut(s) 584, 1118, 1453, 2424
MmeI TCCRAC 2 cut(s) 60, 2887
Mox20I TGGCCA 1 cut(s) 1869
MroI TCCGGA 1 cut(s) 2776
MroXI GAANNNNTTC 2 cut(s) 595, 2505
MscI TGGCCA 1 cut(s) 1869
MslI CAYNNNNRTG 1 cut(s) 2442
Msp20I TGGCCA 1 cut(s) 1869
MspA1I CMGCKG 7 cut(s) 275, 479, 1100, 1940, 1955, 2030, 2054
MspCI CTTAAG 2 cut(s) 14, 1811
MspI CCGG 8 cut(s) 973, 999, 1663, 1794, 1802, 2096, 2539, 2777
MspR9I CCNGG 8 cut(s) 643, 1000, 1074, 1249, 1664, 2097, 2278, 2952
MunI CAATTG 3 cut(s) 377, 906, 2247
Mva1269I GAATGC 1 cut(s) 597
MvaI CCWGG 5 cut(s) 643, 1074, 1249, 2278, 2952
MvnI CGCG 4 cut(s) 321, 1578, 2475, 2598
NciI CCSGG 3 cut(s) 1000, 1664, 2097
NcoI CCATGG 1 cut(s) 2867
NlaIV GGNNCC 5 cut(s) 137, 408, 868, 1003, 2775
NmuCI GTSAC 3 cut(s) 637, 2242, 2696
NspI RCATGY 2 cut(s) 829, 1442
NspV TTCGAA 2 cut(s) 230, 2973
PaeR7I CTCGAG 3 cut(s) 130, 340, 2740
PagI TCATGA 1 cut(s) 1564
PasI CCCWGGG 1 cut(s) 2277
PciI ACATGT 1 cut(s) 825
PctI GAATGC 1 cut(s) 597
PdmI GAANNNNTTC 2 cut(s) 595, 2505
PflFI GACNNNGTC 1 cut(s) 533
PfoI TCCNGGA 1 cut(s) 2950
Ple19I CGATCG 1 cut(s) 2306
PleI GAGTC 4 cut(s) 583, 1118, 1453, 2424
PpsI GAGTC 4 cut(s) 583, 1118, 1453, 2424
PpuMI RGGWCCY 1 cut(s) 135
PscI ACATGT 1 cut(s) 825
PshAI GACNNNNGTC 1 cut(s) 2007
Psp124BI GAGCTC 1 cut(s) 2850
Psp5II RGGWCCY 1 cut(s) 135
Psp6I CCWGG 5 cut(s) 641, 1072, 1247, 2276, 2950
PspGI CCWGG 5 cut(s) 641, 1072, 1247, 2276, 2950
PspN4I GGNNCC 5 cut(s) 137, 408, 868, 1003, 2775
PspPI GGNCC 5 cut(s) 135, 209, 703, 803, 1002
PspPPI RGGWCCY 1 cut(s) 135
PspXI VCTCGAGB 3 cut(s) 130, 340, 2740
PstI CTGCAG 4 cut(s) 67, 1936, 2053, 2755
PstNI CAGNNNCTG 2 cut(s) 1955, 2033
PsuI RGATCY 3 cut(s) 406, 1445, 2947
PsyI GACNNNGTC 1 cut(s) 533
PvuI CGATCG 1 cut(s) 2306
PvuII CAGCTG 6 cut(s) 275, 479, 1100, 1955, 2030, 2054
RsaI GTAC 4 cut(s) 337, 1789, 1999, 2637
RsaNI GTAC 4 cut(s) 336, 1788, 1998, 2636
RseI CAYNNNNRTG 1 cut(s) 2442
SacI GAGCTC 1 cut(s) 2850
SalI GTCGAC 1 cut(s) 2746
Sau96I GGNCC 5 cut(s) 135, 209, 703, 803, 1002
ScaI AGTACT 2 cut(s) 1789, 1999
SchI GAGTC 4 cut(s) 584, 1118, 1453, 2424
ScrFI CCNGG 8 cut(s) 643, 1000, 1074, 1249, 1664, 2097, 2278, 2952
SduI GDGCHC 2 cut(s) 463, 2850
SfcI CTRYAG 5 cut(s) 63, 1932, 2049, 2070, 2751
Sfr274I CTCGAG 3 cut(s) 130, 340, 2740
SfuI TTCGAA 2 cut(s) 230, 2973
SinI GGWCC 3 cut(s) 135, 803, 1002
SlaI CTCGAG 3 cut(s) 130, 340, 2740
SmiMI CAYNNNNRTG 1 cut(s) 2442
SmlI CTYRAG 7 cut(s) 14, 130, 340, 1461, 1811, 2572, 2740
SmoI CTYRAG 7 cut(s) 14, 130, 340, 1461, 1811, 2572, 2740
SsiI CCGC 6 cut(s) 496, 1940, 1947, 1983, 2563, 2598
SspI AATATT 1 cut(s) 567
SspMI CTAG 9 cut(s) 213, 333, 399, 707, 1140, 1152, 1550, 1785, 2366
SstI GAGCTC 1 cut(s) 2850
StyD4I CCNGG 8 cut(s) 641, 998, 1072, 1247, 1662, 2095, 2276, 2950
StyI CCWWGG 4 cut(s) 1533, 2141, 2292, 2867
TaaI ACNGT 7 cut(s) 794, 857, 1648, 1887, 2071, 2642, 3006
TaiI ACGT 2 cut(s) 1386, 1863
TatI WGTACW 3 cut(s) 1787, 1997, 2635
TauI GCSGC 3 cut(s) 1943, 1949, 1985
TscAI CASTG 3 cut(s) 1012, 2044, 2155
TseFI GTSAC 3 cut(s) 637, 2242, 2696
Tsp45I GTSAC 3 cut(s) 637, 2242, 2696
TspRI CASTG 3 cut(s) 1012, 2044, 2155
Tth111I GACNNNGTC 1 cut(s) 533
Vha464I CTTAAG 2 cut(s) 14, 1811
VpaK11BI GGWCC 3 cut(s) 135, 803, 1002
XagI CCTNNNNNAGG 1 cut(s) 1821
XapI RAATTY 9 cut(s) 187, 679, 727, 1258, 1331, 1364, 1709, 2399, 2501
XbaI TCTAGA 2 cut(s) 1151, 2365
XceI RCATGY 2 cut(s) 829, 1442
XcmI CCANNNNNNNNNTGG 1 cut(s) 877
XhoI CTCGAG 3 cut(s) 130, 340, 2740
XmiI GTMKAC 1 cut(s) 2747
XmnI GAANNNNTTC 2 cut(s) 595, 2505
XspI CTAG 9 cut(s) 213, 333, 399, 707, 1140, 1152, 1550, 1785, 2366
ZrmI AGTACT 2 cut(s) 1789, 1999
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.